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  • mRNA
  • Myc mRNA contains an IRES (internal ribosome entry site) that allows the RNA to be translated into protein when 5' cap -dependent translation is inhibited, such as during viral infection. (wikipedia.org)
  • Effects of extremely low frequency electromagnetic field and cisplatin on mRNA levels of some DNA repair genes. (cancerindex.org)
  • The effects of different exposure patterns of 50-Hz EMF and co-treatment of EMF plus cisplatin (CDDP) on mRNA levels of seven genes involved in DNA repair pathways (GADD45A, XRCC1, XRCC4, Ku70, Ku80, DNA-PKcs and LIG4) were evaluated. (cancerindex.org)
  • GADD45A mRNA levels in MCF-7 and SH-SY5Y cells co-treated with CDDP+EMF were increased and at the same time the mRNA levels of XRCC4, Ku80, Ku70 and DNA-PKcs were down-regulated. (cancerindex.org)
  • DNA-activated protein kinase functions in a newly observed S phase checkpoint that links histone mRNA abundance with DNA replication. (sheffield.ac.uk)
  • Eukaryotic initiation factor 4E (eIF4E) binding proteins (4E-BPs) regulate the assembly of initiation complexes required for cap-dependent mRNA translation. (asm.org)
  • The principal control point in mRNA translation is the initiation process, which is largely regulated through changes in the states of phosphorylation of proteins (eukaryotic initiation factors [eIFs] and other components) involved in this process (for reviews see references 11 , 31 , 34 ). (asm.org)
  • 4E-BP1 undergoes phosphorylation at five well-characterized S/T-P sites (Thr36, -45, and -69 and Ser64 and -82 [numbering according to the rat sequence]) in response to agents that activate mRNA translation, e.g., insulin (reviewed in references 11 , 21 ). (asm.org)
  • this was accompanied by increased expression of MSH2 mRNA and protein. (omicsonline.org)
  • Antigen
  • Gottliebanya M, Jackson Stephen P. The DNA-Dependent Protein Kinase: Requirement for DNA Ends and Association with Ku Antigen. (springer.com)
  • The reduction in Ku70 expression correlated significantly with decreased serum prostate-specific antigen (PSA) levels after castration, suggesting that androgen receptor activity regulates Ku70 protein levels in prostate cancer tissue. (aacrjournals.org)
  • 1992
  • He returned to the UK as Principle Investigator at the MRC Protein Phosphorylation Unit in 1992, and subsequently moved to Sheffield to take up a Chair in Cell Biology in the Department of Biomedical Science, serving as Head of Department from 2003 until 2006. (sheffield.ac.uk)
  • Ku70
  • Furthermore, a significant correlation between the reductions of Ku70 after castration versus changes induced of castration of γ-H2AX foci could be seen implicating a functional linkage of decreased Ku70 levels and impaired DNA repair. (aacrjournals.org)
  • Castration therapy results in decreased levels of the Ku70 protein in prostate cancer cells. (aacrjournals.org)
  • Because the Ku70 protein is essential for the NHEJ repair of DSBs and its downregulation impairs DNA repair, this offers a possible explanation for the increased radiosensitivity of prostate cancer cells following castration. (aacrjournals.org)
  • M059J
  • METHODS AND MATERIALS: A DNA-PK activity-deficient human glioblastoma cell line M059J, ataxia telangiectasia cell lines AT3BISV and AT5BIVA, and control cell lines were used. (biomedsearch.com)
  • M059K cells express normal levels of DNA-dependent protein kinase while M059J cells lack DNA-dependent protein kinase activity. (atcc.org)
  • eukaryotic
  • Because A-T cells exhibit a delay in ionizing radiation-induced RPA phosphorylation, our results indicate a functional similarity between MEC1 and ATM, and suggest that RPA phosphorylation is involved in a conserved eukaryotic DNA damage-response pathway defective in A-T. (pnas.org)
  • breaks
  • Gorczyca, Gong J, Darzynkiewicz Z. Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. (springer.com)
  • DNA-PK normally coordinates the repair of simultaneous breaks in both strands of molecules that comprise DNA. (sparkpeople.com)
  • Nevertheless, the scientists discovered, the DNA breaks during HIV integration surprisingly activate DNA-PK, which then performs an unusually destructive role: eliciting a signal that causes the CD4+ T cell to die. (sparkpeople.com)
  • terminus
  • Upon cleavage, the C-terminus of Myc (containing the DNA binding domain ) is degraded, while Myc-nick, the N-terminal segment 298-residue segment remains in the cytoplasm . (wikipedia.org)
  • However, a chimeric protein that was generated by replacing the N terminus of 4E-BP3 with the N-terminal sequence of 4E-BP1 (containing this RAIP motif) underwent a higher degree of phosphorylation and was released from eIF4E. (asm.org)
  • binds
  • Isolation of cDNAs encoding a human protein that binds selectively to DNA modified by the anticancer drug cis-diamminedichloroplatinum(II). (springer.com)
  • Characterization of a DNA damage-recognition protein from mammalian cells that binds to intrastrand d(GpG) and d(ApG) DNA adducts of the anticancer drug cisplatin. (springer.com)
  • eIF4E also interacts with the scaffold protein eIF4G, which in turn binds RNA helicase eIF4A to form the eIF4F complex ( 32 ). (asm.org)
  • JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain. (springermedizin.de)
  • dual-specificity
  • Carl was a co- discoverer of the mechanism of glycogen biogenesis, the identification of a novel class of dual specificity protein phosphatases and has made significant contributions to our understanding of how cell cycle checkpoints are regulated. (sheffield.ac.uk)
  • interacts
  • Through its bHLH DNA-binding motif, Myc interacts with DNA , while the leucine zipper TF-binding motif allows the dimerization with its partner Max, another bHLH transcription factor. (wikipedia.org)
  • activity
  • As the N-terminal Y177 and CC domains from BCR encode the constitutive activation of the ABL1 kinase, these regions are targeted in therapies to downregulate BCR-ABL kinase activity. (wikipedia.org)
  • NHEJ
  • Einzelstrangbrüche der DNA werden durch die Basenexzision repariert und hochmutagene Doppelstrangbrüche schließlich durch eine Reihe komplexer Signalwege auf Grundlage der homologen Rekombination (HR) mit dem Schwesterchromatid (in der S- oder G2-Phase des Zellzyklus) oder der nicht-homologen Endverknüpfung (non-homologous end-joining, NHEJ). (antikoerper-online.de)
  • Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. (abcam.com)
  • induces
  • This is due to the replacement of the myristoylated cap region, which when present induces a conformational change rendering the kinase domain inactive, with a truncated portion of the BCR protein. (wikipedia.org)