Cryoprotectant mixtures have been used for vitrification (i.e. solidification without crystal ice formation). (wikipedia.org)
Dimethyl sulfoxide (DMSO) is also regarded as a conventional cryoprotectant. (wikipedia.org)
In cryobiology DMSO has been used as a cryoprotectant and is still an important constituent of cryoprotectant vitrification mixtures used to preserve organs, tissues, and cell suspensions. (brumer.com)
The present study was designed to determine if honey can be used as a nonpermeating cryoprotectant in vitrification of bovine oocytes. (bvsalud.org)
Vitrification, an alternative cryopreservation tool, has recently been shown to prevent ice formation and involves the use of a high concentration of CPAs and extremely high cooling rate. (molcells.org)
This review presents some of the most noticeable aspects related with the oocyte cryopreservation procedures, emphasizing their evolution in the bovine, which points towards the critical points determining the reduced survival rates of female gametes to freezing and vitrification. (camkkinases.com)
Oocytes2
Glycerol and DMSO have been used for decades by cryobiologists to reduce ice formation in sperm, oocytes, and embryos that are cold-preserved in liquid nitrogen. (wikipedia.org)
Levels of maturation-promoting factor (MPF) in oocytes decline after vitrification, and this decline has been suggested as one of the main causes of low developmental competence resulting from cryoinjury. (molcells.org)
Cryoprotectants2
Mixtures of cryoprotectants have less toxicity and are more effective than single-agent cryoprotectants. (wikipedia.org)
There is a demand for DMSO-free cryoprotectants that maintain cell viability and function after thaw. (nature.com)
Organs1
Vitrification has important applications in preserving embryos, biological tissues and organs for transplant. (wikipedia.org)
Mammalian1
In this study, six DESs were explored for their cryoprotective capacity towards mammalian cells, which included various ratios of mixtures of galactose (Gal) or glycerol (Gly) with either choline chloride (ChCl), betaine (Bet), or proline (Prol) [4]. (tomorrow.bio)
Embryos3
Another drawback of oocyte vitrification is diminished developmental potential because of the decreased quality of embryos after warming. (molcells.org)
Embryos were exposed to 10% EG and 10% DMSO for 1 min and 30 s, and then to 20% EG, 20% DMSO and 0.5 M sucrose for 30 s, always at room temperature. (gsk-3inhibitors.com)
Hematopoietic2
It is particularly important in the freezing and long-term storage of embryonic stem cells and hematopoietic stem cell, which are often frozen in a mixture of 10% DMSO and 90% fetal calf serum. (brumer.com)
As part of an autologous bone marrow transplant the DMSO is re-infused along with the patient's own hematopoietic stem cell. (brumer.com)
Viability1
Results demonstrated that the combination of proline and glycerol (Prol-Gly) was almost as effective as DMSO without a significant reduction in cellular viability post-thaw, even with an extended incubation prior to freezing [4]. (tomorrow.bio)
Kidney1
Twenty-First Century Medicine has vitrified a rabbit kidney to -135 °C with their proprietary vitrification cocktail. (wikipedia.org)
Preservation1
This practice adds to the complexity of the workflow associated with preservation of cells using DMSO. (nature.com)
Solution3
Sugars are commonly supplemented into vitrification solution to dehydrate cells in order to reduce the formation of fatal intracellular ice crystals. (bvsalud.org)
In the second experiment, the immature cumulus-oocyte complexes (COCs) were vitrified in an EG/DMSO-based vitrification solution containing honey (0.5, 1 or 1.5 M) or sucrose (0.5 M) as a gold standard. (bvsalud.org)
For toxin extraction, the filtered solution was poured into glass column contained with 2.5 g florisil, later consecutively poured with 5 ml chloroform, 20 ml methanol and the mixture of acetonitril and distilled water (ration 49:1). (cipav.org.co)
Cells1
Nutrient Mixture F-10 Ham supports the clonal growth of a wide variety of cells like clones of Chinese hamster ovary [CHO] cells and a clone of HeLa and mouse L-cells. (sigmaaldrich.com)
Groups1
The methyl groups of DMSO are somewhat acidic in character (pKa=35) due to the stabilization of the resultant anions by the sulfoxide group. (brumer.com)
Lead1
They protect against ice formation and lead to vitrification , which is the transformation of a substance into a glass-like amorphous state. (tomorrow.bio)
Patients1
Sixty-seven patients Dibutyryl-cAMP solubility dmso (71 procedures, including 4 revisions) underwent the EETSA with bilateral nasoseptal flap elevation. (camkkinases.com)