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  • lineage
  • Exhausted T cells are a discrete cell lineage that have become important immunotherapy targets for chronic infection and cancer,' said senior author John Wherry, PhD , a professor of Microbiology and director of the Institute for Immunology. (news-medical.net)
  • Here we found that the helper T cell fate was not fixed and that mature, antigen-stimulated CD4(+) T cells terminated expression of the gene encoding ThPOK and reactivated genes of the CD8 lineage. (nih.gov)
  • Clear cell carcinoma is an IL-6 driven tumour and lineage experiments on bronchial epithelium have shown that IL-6 is an essential pathway in maintaining the population of ciliated cells. (ubc.ca)
  • Taken together we hypothesize that endometrioid carcinomas are derived from cells of secretory cell lineage whereas clear cell carcinomas are derived from cells of endometrial origin that share features with a ciliated cell lineage. (ubc.ca)
  • To be called an HSC clone, the clone signature (lentivirus insertion locus) had to be present in one short-lived and one long-lived mature blood cell lineage and present at more than a single time period of analysis, one of which had to be later than 1 year after transplant. (sciencemag.org)
  • Abstract
  • Department of Anatomical Pathology, Vancouver General Hospital, Vancouver, BC, Canada Running Title: Distinct cellular origins for endometrioid and clear cell cancers Abstract Endometrial epithelium is the presumed tissue of origin for both eutopic and endometriosis-derived clear cell and endometrioid carcinomas. (ubc.ca)
  • subset
  • The majority of cells within the CD11c + CD11b dull/− subset express high levels of CD8α and DEC-205, markers that are expressed on lymphoid-related DC subset in mice ( 17 - 21 ). (pnas.org)
  • Cells within the CD11c + CD11b bright subset do not express CD8α or DEC-205 but do express F4-80, 33D-1, and other myeloid-related markers, suggesting that they might be of myeloid origin ( 15 , 16 ). (pnas.org)
  • Unexpectedly, TM invariably induced Cre activity in a subset of cells associated with the trabecular bone surface of long bones. (duke.edu)
  • Because T cells consist of a variety of subpopulations, numerous studies have attempted to associate an impaired balance between each T cell subset and periodontal tissue destruction in periodontitis patients. (springer.com)
  • All three types of T cell-stromal cell interactions occur after seeding of the intrathymic precursor cell subset and before development of mature medullary-type T cells. (biomedsearch.com)
  • Here, using in vivo time-lapse imaging of scl isoform-specific reporter transgenic zebrafish lines, we show that prior to EHT scl-β is selectively expressed in hemogenic endothelial cells, a unique subset of VAE cells possessing hemogenic potential, whereas scl-α is expressed later in nascent HSCs as they egress from VAE cells. (biologists.org)
  • mice
  • We used novel, conditionally mutant Tlr4(fl/fl) mice to define the relative contributions of AEC and hematopoietic cell Tlr4 expression to LPS- and allergen-induced airway inflammation. (sigmaaldrich.com)
  • a ) Frequency of GFP positive cells among gated CD45 + TCRβ + lymphocytes isolated from the spleen and mLN of naïve ThPOK -gfp reporter mice. (nih.gov)
  • b ) Frequency of GFP positive cells among gated CD45 + TCRβ + lymphocytes isolated from the small intestine epithelium of naïve ThPOK -gfp reporter mice. (nih.gov)
  • Values in plots indicate percent positivity ( f ) MFI for Granzyme B expression by CD4+, CD8α + CD4 + or CD8αα + T cells in gated CD45 + TCRβ + CD8β − lymphocytes from different tissues of naïve wild type B6 mice. (nih.gov)
  • Seeding of distinct intrathymic microenvironments defined by direct thymocyte-stromal cell interactions was correlated with T cell development in situ using radiation and nonradiation chimeras of Thy-1.1/1.2 congenic mice. (biomedsearch.com)
  • During pneumococcal pneumonia, Miwi2-deficient mice exhibited increased expression of inflammatory mediators and increased immune cell recruitment, leading to enhanced bacterial clearance. (jci.org)
  • B ) Quantitative real-time PCR of RNA isolated from FACS-sorted cells from mice infected i.t. with S . pneumoniae . (jci.org)
  • vitro
  • These cells, when genetically marked and cultured in vitro, were capable of producing bone nodules. (duke.edu)
  • The heterogeneity of arterial SMCs has been established in vitro by isolating cell populations from different locations or from the same location in animals of different ages. (ahajournals.org)
  • 22 23 Epithelioid and spindle-shaped clones can be recovered, albeit in different proportions, from both the normal media and the IT 15 days after endothelial injury, 21 thus providing evidence that in normal conditions, the arterial media contains cells capable of developing in vitro the 2 phenotypes. (ahajournals.org)
  • Because nearly all results suggesting SMC heterogeneity have been obtained in vitro, we wondered whether the distinct SMC phenotypes are permanent or are temporarily preserved by culture conditions. (ahajournals.org)
  • stromal cells
  • In in situ hybridization of the kidney, N-myc autoradiographic grains were primarily located over epithelially differentiating mesenchyme while most of the mesenchymal stromal cells showed only a background signal with the N-myc probe. (rupress.org)
  • Cytoplasm
  • Pathologically, all TCEB1-mutated tumors shared characteristic features including thick fibromuscular bands transecting the tumor, pure clear cell cytology frequently with cells showing voluminous cytoplasm, and clear cell renal cell carcinoma-like acinar areas associated with infolding tubular and focally papillary architecture. (nih.gov)
  • The presence of voluminous cytoplasm, absence of luminal polarization of tumor nuclei, and lack of extensive cup-like distribution of carbonic anhydrase-IX expression distinguish it from clear cell papillary carcinoma. (nih.gov)
  • genes
  • However, the apparent coexpression of the IGF-II and N-myc genes in immature kidneys occurs largely in distinct cell types. (rupress.org)
  • We have shown previously that miR-15a and miR-16 - 1 are located at chromosome 13q14.3 within a 30-kb region of loss in CLL cells and that both genes are deleted and/or down-regulated in the majority of the analyzed CLL cell samples ( 4 ). (pnas.org)
  • Also shown is the strategy to express wide type (WT) or K63R ubiquitin genes fused to ribosomal subunits (L40 and S27a) in the U2OS-shUb cells. (nih.gov)
  • B ) U2OS-shUb-Ub(WT) (lanes 1-4) or U2OS-shUb-Ub(K63R)(lanes 5-8) cells were treated with tetracycline (1μg/ml) for 4 days, then total RNA was isolated for RT-PCR analyses of endogenous Ub genes or exogenous Ub transgene (Ub r ) expression. (nih.gov)
  • Mechanisms
  • Mechanisms of cell interaction with fibronectin have been studied with proteolytic fibronectin fragments that have well-defined ligand binding properties. (rupress.org)
  • Applying this type of assessment to exhausted T cells in the context of immunotherapy clinical trials might identify patients more likely to benefit from specific types of combination immunotherapies and may point to underlying mechanisms in the specific types of exhausted T cells responding to an infection or cancer. (news-medical.net)
  • A ubiquitin replacement strategy in human cells reveals distinct mechanisms of IKK activation by TNFalpha and IL-1beta. (nih.gov)
  • These results indicate that distinct ubiquitin-dependent mechanisms are employed for IKK activation by different pathways. (nih.gov)
  • Understanding the developmental pathway through which HSCs arise and the associated molecular mechanisms would therefore provide new insights into stem cell biology and HSC-based regenerative therapies. (biologists.org)
  • myeloid
  • The hematopoietic stem cell (HSC) derived from adult bone marrow (BM) is commonly thought to have multilineage potential, meaning that the HSC is considered capable of reconstituting all lymphoid, myeloid, and erythroid lineages of the immune system ( 1 , 2 ). (pnas.org)
  • lymphocytes
  • For many years, Dr. Ohashi's team has been studying tumor-infiltrating lymphocytes (TILs), which are the cell type known to kill cancer cells. (eurekalert.org)
  • In the mouse, most (or all) complement-receptor lymphocytes and theta-bearing lymphocytes are part of nonoverlapping populations of cells. (sciencemag.org)
  • Transcriptional reprogramming of mature CD4⁺ helper T cells generates distinct MHC class II-restricted cytotoxic T lymphocytes. (nih.gov)
  • TCRαβ thymocytes differentiate into either CD8αβ(+) cytotoxic T lymphocytes or CD4(+) helper T cells. (nih.gov)
  • natural killer cells (CD16+) and B lymphocytes (CD19+) were purified by using magnetic beads (Dynabeads, Unipath, Milan) and specific mAbs (Becton Dickinson). (pnas.org)
  • demonstrate
  • Studies here finally demonstrate that individual HSCs sorted from adult bone marrow and transferred to lethally irradiated recipients clearly give rise to B-2, MZ B, and B-1b, but does not detectably reconstitute B-1a cells. (pnas.org)
  • lymphoid
  • Transferring adult BM into lethally irradiated recipients readily reconstitutes B-2 and MZ, which represent the majority of the B cells in spleen and other lymphoid organs but only poorly reconstitute B-1 cells in the same recipients. (pnas.org)
  • Lymphoid-Like Structures with Distinct B Cell Areas in Kidney Allografts are not Predictive for Graft Rejection. (sigmaaldrich.com)
  • In addition, it is probable that, although receptors for complement and membrane-bound immunoglobulins coexist on the same lymphoid cells, these markers are located at separate sites on the membrane. (sciencemag.org)
  • thymic
  • Seeding of thymic microenvironments defined by distinct thymocyte-stromal cell interactions is developmentally controlled. (biomedsearch.com)
  • Despite similar capacities to acquire MHC-peptide complexes, thymic CD8α + cDC elicited increased T cell stimulation relative to SIRPα + cDC. (jimmunol.org)
  • APC in the thymic medulla play an essential role in establishing T cell self-tolerance. (jimmunol.org)
  • carcinomas
  • Integrated sequencing analysis identified a group of tumors among clear cell renal cell carcinomas characterized by hotspot mutations in TCEB1 (a gene that contributes to the VHL complex to ubiquitinate hypoxia-inducible factor). (nih.gov)
  • Clear Cell and Endometrioid Carcinomas : are their differences attributable to distinct cells of origin? (ubc.ca)
  • Clear Cell and Endometrioid Carcinomas : are their. (ubc.ca)
  • Endometrial epithelium is the presumed tissue of origin for both eutopic and endometriosis-derived clear cell and endometrioid carcinomas. (ubc.ca)
  • human
  • Here we used cell collapse and stripe assays, showing contrasting effects of human ephrinA5 binding to EphA2 and EphA4. (nih.gov)
  • The N-myc proto-oncogene and IGF-II growth factor mRNAs are expressed by distinct cells in human fetal kidney and brain. (rupress.org)
  • The detection by immunofluorescence of distinct cell populations producing interleukin-1 alpha and interleukin-1 beta in activated human peripheral blood. (ox.ac.uk)
  • An immunofluorescent staining method using specific monoclonal antibodies was used to detect IL-1 alpha and IL-1 beta in individual cells in stimulated human peripheral blood. (ox.ac.uk)
  • This study demonstrates a method for the detection of individual IL-1 alpha- and IL-1 beta- producing cells and suggests that in activated human peripheral blood IL-1 alpha and IL-1 beta are produced by separate populations of cells. (ox.ac.uk)
  • Design Whole-cell patch clamp recordings were obtained from intact human colonic crypts. (bmj.com)
  • Scientists have for the first time grown embryos that contain a combination of pig and human stem cells, in a step toward one day growing transplantable organs, a study said on Thursday. (stem-cells-news.com)
  • We now report the genomewide expression profiling of miRNAs in human B cell chronic lymphocytic leukemia (CLL) by using a microarray containing hundreds of human precursor and mature miRNA oligonucleotide probes. (pnas.org)
  • Here, we devise a tetracycline-inducible RNAi strategy to replace endogenous ubiquitin with a K63R mutant in a human cell line. (nih.gov)
  • Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Cell Biology, Southern Medical University, Guangzhou 510515, P.R. China. (biologists.org)
  • HSCs
  • These differences in B-cell reconstitution capabilities of adult versus neonatal BM underlie the idea that B-1 and B-2 belong to distinct developmental lineages derived from distinct HSCs ( 13 ). (pnas.org)
  • pathogenesis
  • Understanding of the molecular pathogenesis of B cell chronic lymphocytic leukemia (CLL), the most common adult leukemia in the Western world, is incomplete. (pnas.org)
  • Treg
  • T cell central tolerance entails both clonal deletion of thymocytes expressing TCR with increased affinity for self-antigen, and development of Foxp3-expressing regulatory CD4 + T cells (Foxp3 + Treg) in the medulla of the thymus. (jimmunol.org)
  • multilineage
  • Thus, at least with respect to B cells, the multilineage potential of the HSC population in adults is more limited than the multilineage potential of the HSC population in neonates ( 5 , 7 - 12 ). (pnas.org)
  • Multilineage engraftment is exclusively driven by CD34 + CD45RA − CD90 + cell fractions. (sciencemag.org)
  • functional
  • This functional dichotomy is controlled by key transcription factors, including the helper T cell master regulator ThPOK, which suppresses the cytolytic program in major histocompatibility complex (MHC) class II-restricted CD4(+) thymocytes. (nih.gov)
  • differences
  • This lack of genomic differences led us to an alternative hypothesis that these cancers arise from distinct cells of origin within endometrial tissue, and it is the cellular context that accounts for their differences. (ubc.ca)
  • therapies
  • For patients, down the road Dr. Ohashi envisions a new era of combined therapies to simultaneously target and kill these suppressive cells while augmenting the immune response against cancer. (eurekalert.org)
  • A recent report published in the medical journal Stem Cell Reports, sheds light on breakthrough research regarding the use of thyroid cells derived from stem cells for new therapies. (stem-cells-news.com)
  • We want to be able to select and tailor immune therapies according to a patient's exhausted T cell pool and its individual characteristics,' Wherry said. (news-medical.net)
  • determinant
  • Chimeric receptors showed that the ectodomain is a major determinant of cell response. (nih.gov)
  • 309:30-33) representing a cell-binding determinant in the 75-kD fragment, and (b) an antibody raised against the 33-kD fragment that binds specifically to that fragment. (rupress.org)
  • receptors
  • b) Crystal lattice assemblies that are compatible with clustering of receptors on the same cell (in cis ) are shown as surface representations for the four crystal structures presented (side and top views). (nih.gov)
  • Therefore, it appears that (a) both PNS and CNS cells have receptors for each cell-binding domain of fibronectin, (b) the receptor(s) for the two domains are distinct, with attachment to the 33-kD fragment being independent of RGDS, and (c) the relative importance of each domain to cell interaction with intact fibronectin is different for CNS and PNS cells. (rupress.org)
  • differentiate
  • In the present study domain-specific reagents were used in inhibition assays to further differentiate cell surface interactions with the two fibronectin domains, and to define the significance of these domains to cell interactions with the intact fibronectin molecule. (rupress.org)
  • different
  • Initial cell attachment to, and neurite extension upon, fibronectin and the two different fragments was evaluated in the presence and absence of the two reagents. (rupress.org)
  • Rodent models have shown that an optimal balance between functionally different T cells is essential in the protection against periodontal tissue destruction. (springer.com)
  • expression
  • Tamoxifen-inducible gene deletion reveals a distinct cell type associated with trabecular bone, and direct regulation of PTHrP expression and chondrocyte morphology by Ihh in growth region cartilage. (duke.edu)
  • Expression of the Col2-Cre transgene in these cells likely reflected the endogenous Colalpha1(II) promoter activity as similar cells were found to express the IIA isoform of Colalpha1(II) mRNA endogenously. (duke.edu)
  • In summary, the present study has not only provided evidence that Ihh signaling directly controls PTHrP expression and chondrocyte morphology in the growth region cartilage, but has also uncovered a distinct cell type associated with the trabecular bone that appears to possess osteogenic potential. (duke.edu)
  • Some mature CD4 T cells do not maintain ThPOK expression in the periphery. (nih.gov)
  • Little else is known about miRNA expression patterns or function in normal or neoplastic cells. (pnas.org)
  • All tumors lacked the clear cell renal cell carcinoma signature 3p loss and contained distinct gene expression signatures. (nih.gov)
  • A ) Cell surface expression of CD45 versus CD34, CD45RA versus CD117, and CD90 versus CD123 on pig-tailed macaque ssBM-derived white blood cells. (sciencemag.org)
  • intermediate
  • N-myc mRNA was detectable throughout the developing brain with a slight accentuation in the intermediate zone cells in between the subependymal and cortical layers. (rupress.org)
  • One (73% of cells) had whole-cell currents dominated by intermediate conductance (IK) K + channels under resting conditions, which developed large CFTR-mediated currents in response to increasing intracellular cAMP. (bmj.com)
  • Centre
  • TORONTO, Canada - Feb. 6, 2017) - Princess Margaret Cancer Centre scientists have discovered a distinct cell population in tumours that inhibits the body's immune response to fight cancer. (eurekalert.org)
  • population
  • Yet, whether EHT initiates stochastically from the uniform endothelial cells or deterministically from a specialized hemogenic endothelial population is not known. (biologists.org)