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  • strain
  • The 16S rDNA analysis revealed 99% nucleotide similarity to that of the type strain of R.rhodochrous (Fig. 2) ( 10 ). (kenyon.edu)
  • oxygenase
  • 2. Role of oxygen in the metabolic regulation : Besides the well known … More role of oxygen in the energy metabolism, oxygen has been shown to play an important physiological role in metabolic regulation through oxygenase-catalyzed reactions. (nii.ac.jp)
  • For the elucidation of the active site structure of another well characterized oxygenase, catechol 2, 3 - dioxygense, we used o-nitrophenol, a competitive inhibitor, and found it a useful active site probe for this enzyme. (nii.ac.jp)
  • iron enzymes
  • His group is also working towards designing functional models for iron enzymes and capturing, observing, and categorizing highly active metal-based intermediates. (wikipedia.org)
  • catalytic
  • Particle size is also an important consideration: smaller particles have been observed to yield catalytic properties more closely approximating those obtained with soluble enzymes. (clicktocurecancer.info)
  • Catalytic oxidation of 3,5-di- t -butyl-catechol (3,5-DTBC) into quinone occurred without the need of an additional base when the metal complex was confined in the porous solid. (rsc.org)
  • 6-9 In particular, the manganese-dependent dioxygenase (MndD) is a system that has attracted much attention since its catalytic mechanism is still under discussion. (rsc.org)
  • The enzyme resembles a boomerang in shape, and can therefore be clearly divided into three domains: two catalytic domains residing at each end of the "boomerang" and a linker domain at the center. (wikipedia.org)
  • The catalytic mechanism of catechol 1,2-dioxygenase was elucidated using a combination of O18 labeling experiments and crystallography. (wikipedia.org)
  • The most widely observed cofactor involved in dioxygenation reactions is iron, but the catalytic scheme employed by these iron-containing enzymes is highly diverse. (wikipedia.org)
  • Microorganisms
  • 2. The method of claim 1, wherein the liquid is exposed to siderophores by passing the liquid over at least one surface on which are immobilized microorganisms that produce and secrete the siderophores. (patentsencyclopedia.com)
  • 9. The method of claim 2, wherein the microorganisms are halophilic bacteria. (patentsencyclopedia.com)
  • biosynthesis
  • Recently, oxygenases have been shown to be involved in the activation of peptide hormones in mammals and in the biosynthesis of nitric oxide (NO), an endothelium derived relaxation factor, therefore, much attention has been paid to this type of enzymes from the stand point of signal transduction in the cells. (nii.ac.jp)
  • XylE
  • In 2010, the Lethbridge iGEM team confirmed that xylE when introduce to E. coli hydrolyzes catechol to 2-HMS demonstrating and that this critical step can successfully be performed to convert toxic chemicals to metabolic intermediates 7 . (igem.org)
  • crude cell
  • This hypothesis was supported by the results from enzyme assays of the crude cell extract of the consortium revealing catechol 1,2-dioxygenase activity which converted catechol and 4-chlorocatechol to cis,cis-muconic acid and 3-chloro-cis,cis-muconic acid respectively. (kent.ac.uk)
  • bacteria
  • 3. The composition of claim 1 wherein the host organism comprises bacteria of the Bacillus or Clostridia genera. (patents.com)
  • nitrogen
  • strain JS42 is able to utilize 2-nitrotoluene (2NT) as its sole carbon, nitrogen, and energy source. (nih.gov)
  • All heteroatoms (N3O) of the ligand are coordinated to the iron center in complex 1 with two pyridine nitrogen atoms on the axial bonds, while one of the pyridyl arms of the ligand is left uncoordinated in complex 2. (diva-portal.org)
  • plasmid
  • The genes coding for these enzymes are located on pVI150, an IncP-2 degradative mega plasmid of this strain. (diva-portal.org)
  • Restriction enzyme digest fragment patterns of plasmid DNA generated with Xhol showed isolates 7/167 and 8/46 contain different sized plasmids, 61 kb and 95 kb, respectively. (waikato.ac.nz)
  • carbon-sulf
  • Glyoxalase I is classified as a carbon-sulfur lyase although, strictly speaking, the enzyme does not form or break a carbon-sulfur bond. (wikipedia.org)