The aim of this paper is to complement this knowledge by investigating the worm's response to a Staphylococcus aureus infection through a 2-dimensional differential proteomics approach. (biomedcentral.com)
Methodology and Principal Findings: Using a proteomics differential approach with two-Dimensional Differential in-Gel Electrophoresis (2D-DIGE), we defined the protein modulations in the midgut of Aedes aegypti that were triggered seven days after an oral infection (7 DPI) with dengue 2 (DENV-2) and chikungunya (CHIKV) viruses. (hal.science)
The aim of the present work is to study the various trends in proteomics especially 2- Dimensional, Differential Gel Electrophoresis and Mass spectroscopy. (ajphs.com)
Proteomics approach (one-dimensional (1D) and two-dimensional (2D) gel electrophoresis) compared differential protein abundance between controls and the osmotic stressed maize plants. (openbiotechnologyjournal.com)
Two-dimensional differential gel electrophoresis (2D-DIGE) and mass spectrometry (MS) based quantitative proteomics were used to study differential protein abundance in various B cell types during B cell maturation. (lu.se)
We've got examined the biomechanism of zebrafish caudal fin regeneration in various moment items according to differential proteomics strategies. (kinasepathway.com)
The array regarding proteome modifications brought on by renewal have been assessed among settings (2 l) and 1, A dozen, Twenty-four, Forty eight, and Seventy two postamputation concerning quantitative differential proteomics investigation according to two-dimensional carbamide peroxide gel electrophoresis matrix-assisted lazer desorption/ionization along with differential in-gel electrophoresis Orbitrap evaluation. (kinasepathway.com)
DIGE14
This study was undertaken to determine if two-dimensional fluorescence difference gel electrophoresis (2D DIGE) analysis of proteins in synaptic membrane lipid rafts revealed age-dependent alterations in levels of raft proteins. (ku.edu)
Of 1046 matched spots on DIGE gels, 94 showed statistically significant differences in levels between old and young rafts, and 87 of these were decreased in aged rafts. (ku.edu)
We have begun evaluation of BND skin cell cultures exposed to varying concentrations of salinity using two-dimensional differential in-gel electrophoresis (DIGE) and cDNA microarray analysis, respectively. (vin.com)
Design We first developed and validated a workflow-including extraction of microbial communities, two-dimensional difference gel electrophoresis (2D-DIGE), and LC-MS/MS-to discover protein signals from CD-associated gut microbial communities. (bmj.com)
Differential regulated proteins were quantified using 2 dimensional difference gel electrophoresis (2D-DIGE) and differentially regulated proteins were identified using matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-ToF MS). Data shows the NHBE showed a dose dependent response to 5-FU treatment. (dcu.ie)
Through 2D-DIGE and mass spectrometry of granulocyte protein from 20 MPN samples, showed differential expression of HSP70 in PV and ET besides other 60 proteins. (biomedcentral.com)
In an initial discovery stage, we conducted a proteomic approach using two-dimensional differential gel electrophoresis (2D-DIGE). (bvsalud.org)
MudPIT) and Fluorescence two-dimensional differential in-gel electrophoresis (2D-DIGE) to identify potential biomarkers of PTL and SPTB. (elsevierpure.com)
To resolve this issue, I developed an experimental approach that combines the sensitivity and specificity of Click Chemistry with the high-throughput capabilities of two-dimensional differential gel electrophoresis (DIGE). (unr.edu)
In this approach, proteins in different samples are metabolically labeled with azido-substrate analogs, the azido-labeled proteins are marked with custom-synthesized Cy3- or Cy5-alkyne probes via Click Chemistry, and then differences in the post-translational modifications between the samples are visualized by multiplexing the fluorescent samples and electrophoresing them in two-dimensional gels using the established DIGE format. (unr.edu)
In the 2D-DIGE method three protein samples differentially labeled with Cy3-, Cy5- and Cy2-dyes can be separated on the same gel. (lu.se)
Unsupervised classification grouped the 45 samples according to their histological classification based on the protein expression profiles created by laser microdissection and two-dimensional difference gel electrophoresis (2D-DIGE). (elsevierpure.com)
This study has successfully profiled the total osmotic stress responsive proteins and revealed the efficiency of proteomic tools in the qualitative detection of differential proteins from maize. (openbiotechnologyjournal.com)
In order to identify miR-663a-regulated genes, we evaluated proteomic changes in miR-663a-overexpressing cells by two-dimensional differential in-gel electrophoresis coupled to mass spectrometric identification of the differentially represented proteins. (unisa.it)
The most common method is two-dimensional polyacrylamide gel electhrophoresis (2D-PAGE). (hindawi.com)
Large-Format Two-Dimensional Polyacrylamide Gel Electrophoresis - Henry Brzeski, Stephen Russell, Tony G. Sullivan, Richard Somiari & Craig Shriver 14. (nhbs.com)
A proteome analysis of mammalian glomeruli was performed in mouse using two-dimensional-gel electrophoresis with separate Coomassie and silver staining and subsequent mass spectrometric identifications. (researchgate.net)
The particular obtained differential proteome and the immediate affiliation of the various healthy proteins could trigger a whole new knowledge of the regeneration mechanism. (kinasepathway.com)
Differentially3
Three months meats were recognized as differentially governed regarding renewal depending on differential in-gel electrophoresis evaluation relating to the manage as well as rejuvenating flesh. (kinasepathway.com)
Our preliminary study using 2D isolates were recovered from liq- new cases throughout the world cu- gel electrophoresis in drug sensitive/ uid nitrogen, and sub-cultured in taneous leishmaniasis (CL) remains resistant strains of L. tropica showed RPMI1640 medium (Gibco/BRL) a serious public health problem in that some proteins were differentially supplemented with 10% fetal bovine numerous countries [1,2]. (who.int)
In order to gain a more comprehensive perspective on the physiology and regulation of the AlTR, we compared differential gene expression and protein content of cells adapted at pH 9.5 and un-adapted cells (pH 7.0) using complementary DNA (cDNA) microarray and two-dimensional (2D) gel electrophoresis, (combined with mass spectrometry) respectively. (essex.ac.uk)
The observed variability between results of cDNA arrays and 2D gel electrophoresis may be accounted for by posttranslational modifications. (essex.ac.uk)
Gene Expression3
Techniques such as microarrays, expressed tag sequencing (EST), serial analysis of gene expression (SAGE), subtractive cloning and differential display (DD), and two-dimensional electrophoresis gel have been extensively used to screen and analyze parallel gene expression. (ejbiotechnology.info)
Differential display analysis of alteration in gene expression / Yong-Jing Cho and Peng Liang -- 4. (edu.au)
9. Analysis of gene expression by two-dimensional gel electrophoresis / A. M. Tolkovsky -- 10. (edu.au)
Centrifugation1
Preparative centrifugation (differential and density gradient). (uc3m.es)
Statistical analysis and mass spectrometry identified 26 proteins with differential expression, of which 14 were functionally linked to c-Myc, AP-1, HIF1A, hepatocyte nuclear factor 4 alpha, or the Ras superfamily (RhoA, CDC42, and Rac1). (elsevierpure.com)
Protein separation was performed by isoelectrofocusing on 18-cm pH 31NL ImmobilineTM Drystrips (IPG strips, GE Healthcare, Chicago, IL, USA) within the first dimension and SDS-PAGE on twelve diverse 8 to 18 acrylamide gels in the second dimension. (esiservizi.com)
Analysis3
Differential levels of representative identified proteins were confirmed by immunoblot analysis. (ku.edu)
Preparation of protein extracts from recalcitrant plant tissues: An evaluation of different methods for two-dimensional gel electrophoresis analysis. (org.in)
These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. (nhbs.com)
Serum or Plasma Sample Preparation for Two-Dimensional Gel Electrophoresis - Anthony G. Sullivan, Stephen Russell, Henry Brzeski, Richard I. Somiari and Craig D. Shriver 7. (nhbs.com)
Protein expression1
Differential protein expression was studied in eight human cell lines representing various maturation stages from early pre-B cell until terminally differentiated plasma cell (see Supplementary Table 1 ). (lu.se)
Samples2
Preparation of Mammalian Tissue Samples for Two-Dimensional Electrophoresis - Frank Witzmann 5. (nhbs.com)
Triple image overlays enable easy visual comparison between the two samples (labeled with Cy3 and Cy5) in relative to internal standard (Stnd) labeled with Cy2 and run on the same gel. (lu.se)
Spectrometry1
Protein spots were resolved from three subcellular fractions, using two-dimensional differential-in-gel-electrophoresis, revealing 31 spots that changed significantly and were identified by liquid-chromatography tandem mass-spectrometry. (montana.edu)
Spots1
Using 2D gel electrophoresis, a total of seven and eight protein spots were revealed in experimental plants under 5% (w/v) and 10% (w/v) PEG treatment respectively while the control plants only expressed one protein. (openbiotechnologyjournal.com)
Expression1
its differential expression was shown in several disorders affecting the CNS, such as Alzheimer's disease, autism spectrum disorder, and schizophrenia. (elte.hu)
Patterns2
The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. (nhbs.com)
Molecular evaluation by 1D gel electrophoresis revealed that the separated protein patterns were highly expressed in the experiments than the controls. (openbiotechnologyjournal.com)
Cells2
Differential Detergent Fractionation of Eukaryotic Cells - Melinda L. Ramsby and Gregory S. Makowski 6. (nhbs.com)
2021) Cell Surface Protein mRNAs Show Differential Transcription in Pyramidal and Fast-Spiking Cells as Revealed by Single-Cell Sequencing. (elte.hu)
Statistical1
Cy2, Cy3, and Cy5 elements of each gel had been individually imaged as described previously [89].Statistical analysisVelos mass spectrometer (ThermoFisher Scientific, Waltham, MA, USA). (esiservizi.com)