Diffusion in agar. Medical search. Frequent questions

Mueller Hinton agar is a type of growth medium used in microbiology to culture bacterial isolates and test their susceptibility to antibiotics. (wikipedia.org)
However, Mueller Hinton agar is made up of a couple of components, including beef extract, acid hydrolysate of casein, and starch, as well as agar to solidify the mixture. (wikipedia.org)
Meanwhile, The composition of Mueller Hinton agar can vary depending on the manufacturer and the intended use, but the medium is generally nutrient-rich and free of inhibitors that could interfere with bacterial growth. (wikipedia.org)
Mueller Hinton agar is commonly used in the disk diffusion method, which is a simple and widely used method for testing the susceptibility of bacterial isolates to antibiotics. (wikipedia.org)
Mueller Hinton agar is particularly useful for testing a wide range of antibiotics, as it has a low content of calcium and magnesium ions, which can interfere with the activity of certain antibiotics. (wikipedia.org)
The composition of Mueller Hinton agar can affect the growth characteristics of bacterial isolates, as well as their response to antibiotics. (wikipedia.org)
More so, careful selection and preparation of Mueller Hinton agar is important for accurate microbiological assays. (wikipedia.org)
The use of Mueller Hinton agar has been critical in the development of antibiotics and in the study of antibiotic resistance. (wikipedia.org)
Mueller-Hinton agar is a microbiological growth medium that is commonly used for antibiotic susceptibility testing, specifically disk diffusion tests. (wikipedia.org)
Mueller-Hinton agar was co-developed by a microbiologist John Howard Mueller and a veterinary scientist Jane Hinton at Harvard University as a culture for gonococcus and meningococcus. (wikipedia.org)
Åhman J, Matuschek E, Kahlmeter G. Evaluation of ten brands of pre-poured Mueller-Hinton agar plates for EUCAST disc diffusion testing. (wikipedia.org)
Use of Mueller-Hinton broth and agar in the germ tube test. (wikipedia.org)
Fernández-Mazarrasa C, Mazarrasa O, Calvo J, del Arco A, Martínez-Martínez L. High concentrations of manganese in Mueller-Hinton agar increase MICs of tigecycline determined by Etest. (wikipedia.org)
silica gel 60-120 mm mesh size, oxalic acid, TLC silica gel coated plate for detection of spots, CDCl 3 for recording NMR spectra, and DMSO for sample preparation for antibacterial susceptibility test, standard antibiotic drug (gentamycin and chlotrimazole), Mueller-Hinton agar, nutrient agar, and saline solution were used as a culture medium during the antibacterial and antifungal test, and Sephadex LH-20 was also used for further purification. (hindawi.com)

How would the appearance of Escherichia coli colonies on nutrient agar differ from colonies of the same species of MacConkey agar? (answerbag.com)
The inoculated broth medium (from day 2) was then sub-cultured every day on blood agar and MacConkey s agar subsequently for 3 days and incubated for another 24 h. (scialert.net)
are readily transmitted via food, water, environmental contacts, pets and from Culture and identification of strains person to person, with morbidity rates in Frozen Shigella and Salmonella strains developing countries 3-to-6-fold higher were subcultured on MacConkey agar than in developed countries [ 1 ]. (who.int)
Urine specimens were cultured on blood and MacConkey agar. (bvsalud.org)

Precipitins were detected using Ouchterlony's agar gel double diffusion assay. (cdc.gov)
Antibacterial and antifungal activity was measured using agar well diffusion assay and broth microdilution methods, respectively against different pathogenic bacteria and fungi. (ijpsonline.com)
Extracts were assayed for antibacterial activity using the agar well diffusion and micro plate dilution assay. (biomedcentral.com)
This concentration, called the minimal bactericidal concentration (MBC), is determined by taking a small sample (0.01 or 0.1 mL) from the tubes used for the MIC assay and spreading it over the surface of a blood agar plate. (medscape.com)

In-vitro antimicrobial activity was performed by disc diffusion method in MH agar medium and MIC test. (amrita.edu)
disc diffusion method and antioxidant activity by Fenton reaction was undertaken. (researchgate.net)
The antibiotic sensitivity test was done by disc diffusion method. (scialert.net)
The disc diffusion method was used for antimicrobial susceptibility testing. (bvsalud.org)

The antibiotic can be diluted in agar or broth, which is then inoculated with the organism. (msdmanuals.com)

In this method, small disks impregnated with different antibiotics are placed on the surface of the agar, and the zone of inhibition around each disk is measured to determine the susceptibility of the bacterial isolate to that antibiotic. (wikipedia.org)
For example, MH agar may be used in the laboratory for the rapid presumptive identification of C. albicans, as an alternative method for germ tube test (Mattie. (wikipedia.org)
Disk Diffusion agar test is a method of choice because it is cost effective, simple, and now routinely used for detection of antibiotic susceptibility. (ac.ir)
A rapid increase in antibiotic resistance in our region made the authors to design a study to compare this traditional method with two different disk diffusion agar tests. (ac.ir)
The extract was also screened against Candida albicans, Escherichia coli, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus (MRSA), in the agar diffusion method. (nih.gov)
These isolates were screened for production of antimicrobial compounds using agar well diffusion method. (ajol.info)
The commonly used disk diffusion method (also known as the Kirby-Bauer test) is appropriate for rapidly growing organisms. (msdmanuals.com)
The disk diffusion method is a second method of determining antibiotic sensitivity (see image below). (medscape.com)
Disk diffusion method. (medscape.com)

1966) disc diffusion technique on Muller Hinton agar using the readymade antibiotics discs ( Table 1 ). (scialert.net)

The aim of this study was to evaluate the antimicrobial activity of repair materials of endodontic use MTA/Bio Angelus®, white Portland cement, white MTA Angelus® and white MTA ProRoot® on Enterococcus faecalis, Escherichia coli and Staphylococcus aureus by the agar diffusion test and the direct contact test. (bvsalud.org)

The extract showed a remarkable inhibition zone against the pathogen in agar well diffusion assays carried out in Petri plates. (ac.be)

The results showed that MTA/Bio Angelus® presented superior antimicrobial activity against all bacteria in the agar diffusion test and in the direct contact test. (bvsalud.org)
Led by Wilhelm Boland, the team of researchers cultivated Enterococcus bacteria for so-called agar diffusion tests. (mpg.de)

With this technique, disks impregnated with various antibiotics are placed on the surface of an agar plate that has been inoculated with the organism isolated from the patient. (medscape.com)

Not all organisms grow sufficiently rapidly for agar gel diffusion techniques. (vin.com)

Both the agar dilution and disk diffusion methods were performed for imipenem, meropenem and ertapenem. (scielo.org.ar)

A total of 180 composite disks were fabricated for eluted component, disk agar diffusion (DAD), and biofilm inhibition tests. (thejcdp.com)
A better diffusion leads to a truer zone of inhibition. (wikipedia.org)
Kirby-Bauer disk diffusion revealed no zones of inhibition for thanaka against the tested microorganisms. (scirp.org)

Kirby-Bauer disk diffusion results: (a) E. coli and (b) S. aureus on MHA with clindamycin and buffer controls and varying concentrations of thanaka disks (values listed in mg/ml) (c) and (d) P. acnes on Brucella blood agar with the clindamycin and buffer controls and varying concentrations of thanaka disks (values listed in mg/ml, c = control, Cl = clindamycin). (scirp.org)
Antibiotic susceptibility testing to selected antibiotics was done using Kirby-Bauer disk diffusion technique. (who.int)

The antimicrobial activity was performed by agar well diffusion methods. (nepjol.info)
The following phenotypic methods were assayed: the double disk synergy test, using boronic acid or clavulanic acid as inhibitors, "combined" disks of carbapenem plus inhibitor (boronic acid, clavulanic acid and both boronic plus clavulanic acid), by using a pre-diffusion technique and the modified Hodge test. (scielo.org.ar)

The aim of the present work was to study in vitro antifungal activity of the essential oil from C. officinalis flowers, as determined by agar disk diffusion, on 23 clinical fungal strains. (scielo.br)
The simulation of combined disks by our pre-diffusion technique detected all KPCpositive strains for all 3 carbapenems when using boronic acid as inhibitor, clavulanic acid was less susceptible and specific as compared with boronic acid. (scielo.org.ar)

This allows for better diffusion of the antibiotics than most other plates. (wikipedia.org)

Sedighi I, Solgi A, Alikhani M Y, Emad Momtaz H, Mihani F. Comparison of Two Different Disk Diffusion Agar Tests in Determination of Antibiotic Susceptibility for E-Coli Isolated from Urinary Tract Infection in Pediatrics. (ac.ir)

Isolates of E. coli (ATCC 25922), S. aureus (ATCC 25923), and P. acnes (clinical isolates identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry) were isolated on 5% sheep-blood agar (Becton Dickinson, Cat# 221261). (scirp.org)
Based on these results, the use of 2× hop gradient agar plates (0-58 BU) is recommended for routinely assessing bacterial hop resistance, with highly hop-resistant isolates requiring a 4× gradient plate (0-116 BU). (asbcnet.org)
The double disk diffusion test using boronic acid could detect all KPC-positive isolates, but adjustment of disk distance was necessary for achieving such performance. (scielo.org.ar)

Antibiogram detection was performed by disk diffusion agar test with two different kits as Padtan-Teb (made in Iran) and Mast (made in the U.K.) for Co-trimoxazol, Amikacin, Ceftriaxone, Nalidixic Acid, Cefixime, and Nitrofurantoin. (ac.ir)

It places antibiotic-impregnated disks on agar plates inoculated with the test organism. (msdmanuals.com)

According to our study the results of antibiotic susceptibility were more compatible with other non national Disk diffusion agar test and thus we recommend that our manufactures in Iran should increase the quality of their products. (ac.ir)
A novel version of this test involves a quantitative diffusion gradient, or Epsilometer (E-test), and uses an absorbent strip with a known gradient of antibiotic concentrations along its length. (medscape.com)

Susceptibility testing of Salmonella using disk diffusion" (PDF). (wikipedia.org)

A major factor for bacterial growth in beer is hop resistance, which we have previously shown can be assessed with hop extract (iso-alpha-acids) infused agar (HGA) plates with a bitterness gradient ranging from 0 to 29 BU. (asbcnet.org)
To make hop agar gradient plates more applicable to a brewery setting, we assessed various parameters, including the steepness of the BU gradient, effect of the presence of ethanol, and stability of hop extract over time. (asbcnet.org)
Diffusion from the bottom agar layer creates a hops gradient at the agar surface. (asbcnet.org)

Lactose-Electrolyte Deficient (CLED) and Blood agar plates, and incubated aerobically at 37oC for 24 hours. (who.int)

This study tested in vitro activity of the essential oil from flowers of Calendula officinalis using disk-diffusion techniques. (scielo.br)
Disk diffusion may not be the best modality for definitive analysis of the antimicrobial activity of thanaka. (scirp.org)

It typically contains: 2.0 g beef extract 17.5 g casein hydrolysate 1.5 g starch 17.0 g agar 1 liter of distilled water. (wikipedia.org)

Pre-diffusion technique. (scielo.org.ar)

HGA plating consists of pouring a dual layer agar plate such that the bottom layer containing the maximum BU concentration forms a 10.5 degree wedge of hop-infused agar, which is then covered by a top layer of hop-free agar. (asbcnet.org)
When the strip is placed on the surface of an agar plate seeded with a microbe to be tested, antibiotic diffuses into the medium. (medscape.com)

Thirty-six fluids from non-keratinizing jaw cysts have been examined together with autologous sera by immunoelectrophoresis and double diffusion in agar or agrose gels. (nih.gov)

The effect of ethanol on hop resistance can be assessed by including ethanol in both agar layers. (asbcnet.org)

L'extrait a montré une zone d'inhibition remarquable contre l'agent pathogène dans des essais de diffusion en gélose. (ac.be)

agar well diffusion and growth curve. (bvsalud.org)

Anatomy14

Organisms18

Diseases5

Chemicals and Drugs43

Analytical, Diagnostic and Therapeutic Techniques and Equipment35

Phenomena and Processes37

Disciplines and Occupations5

Technology, Industry, Agriculture5

Information Science9

Health Care8

Mueller-Hinton14

MacConkey4

Assay4

Disc diffusion method4

Broth1

Method9

Hinton1

Escherichia1

Assays1

Bacteria2

Inoculated with the organism1

Organisms1

Dilution1

Inhibition3

Kirby-Bauer2

Methods2

Strains2

Antibiotics1

Determination1

Isolates3

Antibiogram1

Disks1

Test2

Susceptibility1

Gradient3

Blood1

Activity2

Extract1

Technique1

Plate2

Double1

Resistance1

Zone1

Growth1