Differentiation substrate cardiomyocytes. Medical search. Frequent questions

Prof. Mummery has pioneered studies on cardiomyocytes from human embryonic stem cells (hPSC) and was among the first to inject them in mouse heart after myocardial infarction. (wikipedia.org)
At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo . (nottingham.ac.uk)
Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells Biophysical Journal. (nottingham.ac.uk)
Electrical stimulation of human embryonic stem cells: Cardiac differentiation and the generation of reactive oxygen species. (unipd.it)
Laminin-221 (LN221) was identified as the most likely expressed cardiac laminin and the authors show that this protein promotes differentiation of pluripotent human embryonic stem cells (hESCs) toward cardiomyocyte lineage and downregulates pluripotency and teratoma-associated genes. (biolamina.com)
While adult stem cells have yet failed to convincingly regenerate myocardial tissue, human embryonic stem cells (hESCs) have proven to be a potential and unlimited source for cardiomyocyte regeneration. (ca.gov)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)

To recapitulate the phenotype of an adult cardiomyocyte, differentiating progenitors need to upregulate mitochondrial glucose and fatty acid oxidation. (ox.ac.uk)
During stem cell cardiac differentiation, a distinct swish in the expression profile of laminin subunits has been reported with laminin-411/421 pre-dominantly expressed early in progenitors and laminin-211/221 expressed later in cardiomyocytes (Ja, 2015). (biolamina.com)
A highly reproducible, chemically defined, xeno-free laminin-based differentiation protocol to generate stem cell-derived cardiovascular progenitors (CVPs) was reported in 2019 (Yap, 2019). (biolamina.com)
We have started pre-clinical studies of transplanting cardiovascular progenitors (at different stages of development) and mature cardiomyocytes in animal models. (ca.gov)
Furthermore, we have shown that transplantation of immature cardiovascular progenitors may generate cardiomyocytes in addition to supporting cells such as vascular endothelial cells and fibroblasts. (ca.gov)

Researchers at Michigan Tech and Harvard Medical School have developed a new way to create cardiomyocytes from induced pluripotent stem cells (iPSCs), for improved cardiac cell therapy. (medgadget.com)
Induced pluripotent stem cell-derived cardiomyocytes beating in substrate. (medgadget.com)
Producing mature and functional cardiomyocytes (CMs) by in vitro differentiation of induced pluripotent stem cells (iPSCs) using only biochemical cues is challenging. (sharif.edu)
Pluripotent stem cell-derived cardiomyocytes (hPSC-CM) have great importance for predicting safety parameters for pharmaceutical compounds and models of healthy versus disease states of the human heart. (edu.au)
This method allows us to visualise how individual proteins can affect cells' behaviour in a larger array whilst highlighting how specific combinations can precondition pluripotent cells towards a cardiomyocyte lineage. (edu.au)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
Curi Bio released its new proprietary ComboMat platform, a human induced pluripotent stem cell cardiomyocyte maturation technology with applications in. (scienceboard.net)
Gibco media, supplements, substrates, growth factors and cytokines provide you with an easy-to-use, flexible set of tools for targeted differentiation of induced pluripotent stem cells. (thermofisher.com)

Ghasemi-Mobarakeh L, Prabhakaran MP, Tian L, Shamirzaei-Jeshvaghani E, Dehghani L, Ramakrishna S. Structural properties of scaffolds: Crucial parameters towards stem cells differentiation. (wjgnet.com)
In 1981, she continued with a Postdoctoral fellowship at the Hubrecht Institute (KNAW), Utrecht, working on neuroblastoma and embryonal carcinoma cells as screens for teratogens before being appointed tenured staff scientist working on developmental biology, differentiation and stem cells in 1985. (wikipedia.org)
Passier, Robert, "Improved cardiomyocyte differentiation", published 31 May 2004 Mummery co-authored "Stem Cells, 2nd edition scientific facts and Fiction" (Elsevier 2014). (wikipedia.org)
Moreover, it seems that paracrine action of skeletal myoblasts facilitate neighboring cardiomyocytes to maintain their replicative potential and/or stimulate differentiation of native cardiac stem cells (8,9). (escardio.org)
The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
September 29, 2020 -- Fujifilm Irvine Scientific said it has become the exclusive worldwide distributor of cellnest, a recombinant peptide attachment substrate that's designed to provide optimal adhesion and proliferation of stem cells for cell and gene therapy research. (scienceboard.net)
Cellnest, which was designed and manufactured by Fujifilm Irvine Scientific's parent company Fujifilm, mimics the extracellular matrix and enables the adhesion, expansion, and potential differentiation of stem cells, according to the vendor. (scienceboard.net)

Interestingly, contradictory data exist for transformation of bone marrow derived progenitor cells (BMPC) into new cardiomyocytes and alternative mechanisms as enhanced neovascularization, enhanced scar tissue formation due to augmented inflammatory response and decreased apoptosis have been suggested for the benefical effects of these cells on myocardial function after myocardial infarction (4). (escardio.org)

In the second part of this thesis, I demonstrate a novel bioreactor that attenuates a magnetic field to dynamically modulate the stiffness of magnetoactive hydrogel to look at how biomimetic dynamic stiffening of a substrate can influence cardiomyocyte lineage specification. (edu.au)
Non-invasive electromagnetic signals affect substrate stiffness when combined with magnetic particles and magnetic fibres and how this can help direct cell orientation and accompanying lineage specification Finally, I investigate how variability in cell phenotypes and expression patterns are influenced by biomimetic cues and how these variabilities could be utilised in future safety assessment protocols and cell therapy treatments for cardiovascular disease. (edu.au)
Hence, upon differentiation of hESCs towards cardiac lineage, a distinct fluorescent color is expressed sequentially at each stage of cardiovascular development. (ca.gov)

Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)

These lab-fabricated, fluorescently stained cardiomyocytes-heart muscle cells-exhibit the maturation and functionality of heart cells grown within a heart. (medgadget.com)
We are using biochemical and biomechanical cues to enhance the differentiation and maturation. (medgadget.com)
To mimic the biophysical and biomechanical complexity of the native in vivo environment during the differentiation and maturation process, polydimethylsiloxane substrates with 3D topography at the micrometer and sub-micrometer levels are developed and used as cell-culture substrates. (sharif.edu)
The results show that while cylindrical patterns on the substrates resembling mature CMs enhance the maturation of iPSC-derived CMs, sub-micrometer-level topographical features derived by imprinting primary human CMs further accelerate both the. (sharif.edu)

mEScell growth and differentiation on PLDLA-co-TMC membrane, demonstrated the cyto compatibility of this polymer, and provided evidence of minimal to no toxicity on the cells, allowing adhesion and cell proliferation, as well as differentiation into cardiomyocytes. (jscimedcentral.com)
Growing the cells in a three-dimensional substrate that mimics the natural heart environment produces better cells. (medgadget.com)
The mechanical properties of substrates play an important role in the cell behavior because the mechanical cues that cells sense in the actual (heart) environment is unique," said Abadi. (medgadget.com)
This results in more robust, mature and functional cardiomyocyte-like cells. (medgadget.com)
As the cells mature, they begin to beat, and more closely resemble mature, native cardiomyocytes. (medgadget.com)
Biochemicalanalyses revealed that H9c2 cells showed different cardiomyocyte phenotypesand distinct levels of maturity depending on the amount of Ppy inthe substrate used. (ibecbarcelona.eu)
Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)
Myocardial infarction (MI) is one of the clusters of numerous other cardiovascular diseases and occurs due to the blockage of the coronary artery delivering blood (ischemia) to the ventricle with consequent oxygen shortage to the contractile cells (cardiomyocytes) [ 1 ] . (encyclopedia.pub)
In the earliest stages of development, the physical properties of the microenvironment can direct cell differentiation, and initiate the coordinated movement of groups of cells to establish the patterns that will define how the body is arranged. (mechanobio.info)
Collagen IV promoted proliferation of CDCs whereas fibronectin primed cells for differentiation towards a cardiac phenotype. (ox.ac.uk)
Laminin-521 supports increased differentiation potential without altering the traditional Pax7/MyoD paradigm and the results are translational across several mouse backgrounds, human cells, and disease states. (biolamina.com)
It is possible that the transplanted cells fail to physiologically couple with the host tissue, or they may modify the substrate such that a pro-arrhythmic focus is created. (ca.gov)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
In the heart, cardiomyocytes interact with neighboring cells through direct cell-cell interactions, various secreted cytokines, and electric signals (Figure 1 A) [ 24 ]. (thno.org)
TLRs are membrane-anchored proteins present in several cell types ranging from macrophages and T and B cells to nonimmune cells such as cardiomyocytes [ 20 , 21 ]. (hindawi.com)
Protocols for hepatocyte differentiation from iPS cells have been studied. (spandidos-publications.com)
Our complete portfolio of stem cell research products and services provides optimized tools for each step of the stem cell workflow: from reprogramming somatic cells to iPSC expansion, validation, and differentiation. (thermofisher.com)
Our differentiation portfolio allows optimal growth, expansion and storage of differentiated cells-enabling faster, more efficient systems. (thermofisher.com)
These cells have been shown to differentiate into cardiomyocytes, neural cells and hepatocytes. (cellsystems.eu)

iPSCs show significant potential for cardiac regenerative medicine, as researchers can use biochemical cues to "program" them into a cardiomyocyte-like cell, which shows regenerative efficacy in damaged cardiac muscle. (medgadget.com)
With the use of cardiac muscle-specific laminin-211, human iPSCs were effectively differentiated into cardiomyocytes using small molecules (Minami, 2012). (biolamina.com)
iPSCs were efficiently expanded and differentiated to cardiomyocytes on laminin-521 (Kuroda, 2015). (biolamina.com)
iPSCs are engineered to analyze disease pathways, better understand gene and protein expression, and study differentiation. (thermofisher.com)

Here we cultured and differentiated CDCs using protocols aimed to maintain stemness or to promote differentiation, including triggering fatty acid oxidation using an agonist of peroxisome proliferator-activated receptor alpha (PPARα). (ox.ac.uk)
This combinatorial approach led to the identification of several unique matrices that promote differentiation, which will aid efforts at producing therapeutically useful cell types with greater efficiency. (edu.au)

Using photolithography to shape a polydimethylsiloxane substrate and micropatterning to alter the substrate surface, the researchers developed 3D molds that mimic the natural forces and pressures that cardiomyocytes experience in the heart. (medgadget.com)

Cardiovascular diseases often cause substantial heart damage and even heart failure due to the limited regenerative capacity of adult cardiomyocytes. (thno.org)

Dr. Joseph Wu´s lab also showed that laminin-521 is an optimal matrix for chemically defined differentiation of human iPSC to cardiomyocytes (Burridge, 2014). (biolamina.com)
Biolaminin 521 is an optimal matrix for chemically defined differentiation of human iPSC to cardiomyocytes. (biolamina.com)

The myocardial nicheplays a critical role in directing the function and fate of cardiomyocytes;therefore, engineering a biomimetic scaffold holds excellent promise.We produced an electroconductive cardiac patch of bacterial nanocellulose(BC) with polypyrrole nanoparticles (Ppy NPs) to mimic the naturalmyocardial microenvironment. (ibecbarcelona.eu)
Herein, we introduce a cardiac-mimetic cell-culture system that resembles the microenvironment in the heart and provides interactions with cardiomyocytes and electrical cues to the cultured fibroblasts for direct cardiac reprogramming. (thno.org)

Laminin-521 supports increased proliferation during expansion and superior differentiation with myotube hypertrophy, larger myotubes and higher amounts of nuclei per myotube. (biolamina.com)

The HSM was modified into a hepatocyte differentiation inducer (HDI). (spandidos-publications.com)

The final result is that, after MI, billions of cardiomyocytes (CMs) with limited proliferation capacity are lost and substituted by heterogeneous collagen-rich fibrotic scar tissue [ 2 ] [ 3 ] . (encyclopedia.pub)

Inflammatory signaling in cardiomyocytes usually occurs as an early response to myocardial injury and entails cytosolic and mainly mitochondrial reactive oxygen species (ROS) overproduction [ 10 , 11 ]. (hindawi.com)
Expression of genes that regulate mitochondrial biogenesis, substrate utilization, and oxidative phosphorylation (OXPHOS) were determined. (omicsdi.org)

ISSCR Public Service Award 2023 Elected member Academia Europaea (2015) Hans Bloemendaal Medal 2014 for innovative interdisciplinary research Hugo van de Poelgeest Prize 2014 for Animal Alternatives Elected member of the Royal Netherlands Academy of Arts and Sciences (KNAW) (elected 2010) Radcliffe Fellowship, Harvard University (2007) Royal Society Fellowship, UK (1978, 1979) Prof. Mummery is part of five different patents on differentiation of cardiomyocytes and hESC. (wikipedia.org)

The human neonatal dermal fibroblasts containing cardiac transcription factors were plated on the membrane and cultured with the murine cardiomyocyte in the presence of the electric stimulus. (thno.org)

Human heart changes its energetic substrates from lactate and glucose to fatty acids during the neonatal period. (techscience.com)
Addition of a PPARα agonist during differentiation increased both glucose and fatty acid oxidation and expression of cardiac genes. (ox.ac.uk)
DCM entails the damage of the myocardium through fibrosis, steatosis, apoptosis, and hypertrophy [ 3 ] and results from the switch of substrate supply to free fatty acids (FFA) that follows the reduced levels of insulin, glucose transporters, and glucose consumption [ 4 , 5 ]. (hindawi.com)
It was hypothesized that the addition of glucose and galactose promoted hepatocyte differentiation of iPS cell. (spandidos-publications.com)

Mature cardiomyocytes have passed the stage of self-renewal and may pose problems due to lack of proper integration. (ca.gov)
In some cases, "master" genes have been described which control differentiation versus self-renewal. (justia.com)

We conclude that oxidative metabolism and cell differentiation act in partnership with increases in one driving an increase in the other. (ox.ac.uk)

Myoblasts retain skeletal muscle properties and couple only sporadically to resident cardiomyocytes precluding synchronized contraction as mechanism of benefit (8). (escardio.org)
This in accordance with recent data presented by researchers at Icagen Inc., demonstrating that the laminin-521 cell culture matrix maintains the differentiation potential of mouse and human satellite cell-derived myoblasts, even during long-term culture expansion (Penton, 2016). (biolamina.com)

and Therapy, Cardiovascular Research, Molecular Therapy & Differentiation. (wikipedia.org)

Cardiomyocytes are the chief cell type in the heart and their coordinated contraction as a mass is responsible for the pumping of blood around the developing embryo. (mechanobio.info)

The proximal events of TLR-mediated intracellular signaling are initiated by interactions with cytosolic adapters, mainly myeloid differentiation primary response protein 88 (MyD88) [ 25 ]. (hindawi.com)

iPSC differentiation requires standardized culture methods to ensure reproducible and reliable results. (thermofisher.com)

Laminin-211 has also been used for the culture of adult human cardiomyocytes (Kuroda, 2015). (biolamina.com)

The same researchers later published another article with Shinya Yamanaka as co-author, where they again used laminin-211 for cardiac differentiation of human iPSC (Hirata, 2014). (biolamina.com)

After two days of culture in HSM, the expression levels of α-fetoprotein (AFP) and albumin, which are hepatocyte markers, were upregulated, suggesting that hepatocyte differentiation was initiated ( 19 ). (spandidos-publications.com)

Reactive PEG reagents are used to derivatize, functionalize, conjugate or crosslink a variety of substrate molecules, particles and surfaces. (creativepegworks.com)

Cell therapy is currently emerging as a potential new treatment for post MI patients with the assumption that recolonization of the areas of scarred myocardium with exogenously supplied surrogates or precursors of cardiomyocytes can restore function and ultimately affect clinical outcomes. (escardio.org)
From feeder-based and feeder-free media to optimized passaging reagents and substrates to media for stem cell therapy research, Gibco products enable you to culture with confidence. (thermofisher.com)

Reversible alteration of calcium dynamics in cardiomyocytes during acute hypoxia transient in a microfluidic platform. (unipd.it)

The team developed a culture mold that mimics the physiological conditions under which cardiomyocytes grow. (medgadget.com)
show that laminin-521 supports more consistent and reliable differentiation over long-term culture and is the only substrate facilitating high-level fusion following long-term culture. (biolamina.com)
Nano-thin and nano-porous membranes in the culture system facilitated interactions between fibroblasts and cardiomyocytes in coculture. (thno.org)
The cardiac-mimetic culture system may serve as an effective tool for producing a feasible number of reprogrammed cardiomyocytes from fibroblasts. (thno.org)

Anatomy50

Organisms12

Diseases9

Chemicals and Drugs81

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes69

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science4

Embryonic9

Progenitors5

Pluripotent8

Stem cells12

Progenitor cells1

Lineage3

Proliferation and differentiation1

Maturation4

Cells21

IPSCs4

Promote differentiation2

Polydimethylsiloxane1

Regenerative1

Optimal2

Microenvironment2

Myotubes1

Inducer1

Tissue1

Mitochondrial2

20231

Murine1

Glucose4

Self-renewal2

Oxidative1

Myoblasts2

Cardiovascular1

Contraction1

Protein1

Methods1

Adult1

Human1

Markers1

Particles1

Therapy2

Dynamics1

Culture4