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  • vitro
  • Currently, the in vitro manipulation and culture of these cells is still in its infancy, yet muscle stem cells may be the most promising route toward the therapy of muscle diseases such as muscular dystrophies. (frontiersin.org)
  • Pax7
  • Pax7 expressing muscle stem cells accompany all skeletal muscles in the body and in healthy individuals, efficiently repair muscle after injury. (frontiersin.org)
  • To shed light on this, we traced the emergence of head muscle stem cells in the key vertebrate models for myogenesis, chicken, mouse, frog and zebrafish, using Pax7 as key marker. (frontiersin.org)
  • Unlike trunk muscle stem cells, head muscle stem cells do not have a previous history of Pax7 expression, instead Pax7 expression emerges de-novo . (frontiersin.org)
  • muscle
  • This is due to the fact that the timing and mechanisms underlying head muscle stem cell development are still obscure. (frontiersin.org)
  • Consequently, it is not clear at which time point one should compare the properties of head mesodermal cells and head muscle stem cells. (frontiersin.org)
  • Our study reveals a common theme of head muscle stem cell development that is quite different from the trunk. (frontiersin.org)
  • We propose that this unique mechanism of muscle stem cell development is a legacy of the evolutionary history of the chordate head mesoderm. (frontiersin.org)
  • However, in a number of diseases including muscular dystrophies, cancer and HIV/Aids, the ability of muscle stem cells to repair muscle is compromised. (frontiersin.org)
  • Stem Cell
  • The latter concept of stimulating the cell cycle, is particularly appealing with the current interest in stem cell therapy for myocardial infarction and heart failure. (ahajournals.org)
  • TRIM32 Regulates Skeletal Muscle Stem Cell Differentiation and Is Nece" by Sarah Nicklas, Anthony Otto et al. (purdue.edu)
  • Here we demonstrate for the first time that TRIM32 is expressed in the skeletal muscle stem cell lineage of adult mice, and that in the absence of TRIM32, myogenic differentiation is disrupted. (purdue.edu)
  • The broad interest of the WAGERS LAB is to identify and analyze tissue-specific stem cell populations in adult animals. (harvard.edu)
  • Importantly, failure or inefficiency in any one of these stages of transplantation can cause failure of the stem cell graft, and so, understanding the molecular and cellular processes that permit these stem cell functions is essential for improving transplant outcomes. (harvard.edu)
  • To this end, we are pursuing both genetic and cell biological approaches to defining genes and gene products that control stem cell migration, expansion, differentiation and survival, in order to devise better engraftment strategies that limit transplant-associated complications. (harvard.edu)
  • In addition, in collaboration with an NIH-funded consortium of investigators interested in applying stem cell transplant-based therapeutics to the treatment of single gene disorders, we also are developing new, robust approaches for precise and directed gene-modification of stem cells, to support the effective use of gene-corrected stem cells for the treatment of sickle cell disease and related disorders. (harvard.edu)
  • Currently, blood-forming stem cells are the only adult stem cell population that has been purified and used for the treatment of human disease. (harvard.edu)
  • Short-term calorie restriction enhances skeletal muscle stem cell function. (harvard.edu)
  • Taking into account the advances in the isolation of new subpopulation of stem cells and in the creation of artificial stem cell niches, we discuss how these emerging technologies offer great promises for therapeutic approaches to muscle diseases and muscle wasting associated with aging. (frontiersin.org)
  • These include resident endothelial cells that restore capillary bed function during sprouting angiogenesis ( 5 ), as well as other stem cell populations from the bone marrow compartment ( 57 ). (physiology.org)
  • This review summarizes STAT3 function and regulation, its role in stem cell and cancer stem cell properties and highlights recent reports about its relationship to p63. (springer.com)
  • Myoendothelial cells also showed no propensity to form tumors, a concern with other stem cell therapies. (pitt.edu)
  • However, because satellite cells are known to be heterogeneous, clonal analysis is required to demonstrate stem cell function. (nih.gov)
  • In addition, we show using in vivo bioluminescence imaging that the dynamics of muscle stem cell behaviour during muscle repair can be followed in a manner not possible using traditional retrospective histological analyses. (nih.gov)
  • Telocytes have been localized in the stem cell niche of different organs such as the heart, lung, skeletal muscle, and skin. (springer.com)
  • In this review, the distribution of telocytes in different stem cell niches will be explored, highlighting the intimate relationship between the two types of cells and their possible functional relationship. (springer.com)
  • The stem-cell niche as an entity of action. (springer.com)
  • Further aims were to characterize both the graft and the epithelial surface of the mouse and rat cornea using putative stem cell markers (P63 and Telomerase) and marker of cell differentiation (14-3-3 sigma). (ebscohost.com)
  • The article offers information on the new MilliTrace stem cell lines expressing green fluorescent protein (GFP) from Millipore Corp. (ebscohost.com)
  • ii) to evaluate which molecular markers which had been described for satellite cell and stem cell identification in different cell states (quiescence, activated or differentiated) are the most useful for studies on human skeletal muscle. (diva-portal.org)
  • This is due to the fact that the timing and mechanisms underlying head muscle stem cell development are still obscure. (frontiersin.org)
  • Our study reveals a common theme of head muscle stem cell development that is quite different from the trunk. (frontiersin.org)
  • We propose that this unique mechanism of muscle stem cell development is a legacy of the evolutionary history of the chordate head mesoderm. (frontiersin.org)
  • This has been ascribed to inflammatory responses, changes to the stem cell niche and changes to the stem cells themselves. (frontiersin.org)
  • gene expression
  • Of note, the inhibition of the myogenic program, by transfected SOD1 G93A gene expression, impinged also the identity of myogenic cells. (hindawi.com)
  • Vole TS-like cells are similar to murine TS cells with respect to their morphology, transcription factor gene expression and differentiation in vitro into derivatives of the trophectoderm lineage, and with respect to their ability to invade and erode host tissues, forming haemorrhagic tumours after subcutaneous injection into nude mice. (jove.com)
  • lineage
  • the DLL1-induced Notch signaling is mediated through an intercellular communication that regulates cell lineage, cell specification, cell patterning and morphogenesis through effects on differentiation and proliferation (PubMed:11581320). (nih.gov)
  • Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor (PubMed:11581320). (nih.gov)
  • These multi-lineage stem cells include side-population cells, muscle-derived stem cells (MDSCs), and mesoangioblasts. (pitt.edu)
  • Moreover, vole TS-like cells carry an inactive paternal X chromosome, indicating that they have undergone imprinted X inactivation, which is characteristic of the trophoblast lineage. (jove.com)
  • 1998. The ability of TS cells to preserve the trophoblast specific property and their expression of stage- and cell type-specific markers after proper stimulation provides a valuable model system to investigate trophoblast lineage development whereby recapitulating early placentation events. (jove.com)
  • transfection
  • Non-viral transfection methods are typically preferred to deliver the transposon components into the target cells. (bioscirep.org)
  • Typically, non-viral transfection requires the introduction of plasmids harboring the transgene expression cassettes into the desired target cells, in the absence of any viral elements. (bioscirep.org)
  • migration
  • A Travelling Fellowship from Journal of Cell Science allowed her to spend time in Prof Maddy Parson's lab at King's College London, learning new cell migration assays and analysing fibroblasts cultured from individuals with Parkinson's. (biologists.org)
  • In regenerating muscle, however, the number of myogenic precursors exceeds that of resident satellite cells, implying migration or recruitment of undifferentiated progenitors from other sources. (sciencemag.org)
  • Extracellular matrix remodeling after proatherosclerotic injury involves an increase in hyaluronan (HA) that is coupled with vascular smooth muscle cell (SMC) migration, proliferation, and with neointima formation. (biomedsearch.com)
  • mechanism
  • The development of diploid stem cells into polyploid trophoblast cells in culture makes this ex vivo system an excellent tool for elucidating the regulatory mechanism of genome replication and instability in health and disease. (jove.com)
  • hematopoietic
  • The success of these transplants depends critically on the surprising ability of intravenously infused hematopoietic stem cells, which normally reside predominantly in the bone marrow, to accurately and efficiently migrate from the blood to the marrow of transplant recipients, whose own blood system has been compromised by radiation and/or chemotherapy. (harvard.edu)
  • The transcription factor EGR1 controls both the proliferation and localization of hematopoietic stem cells. (harvard.edu)
  • Hematopoietic and CD45 − /VEGFR2 + cells (known as endothelial progenitor cells or circulating angiogenic cells) have been reported to contribute to angiogenesis through the paracrine secretion of proangiogenic factors, as well as incorporate into new vessels, although luminal incorporation appears to be a relatively rare event ( 48 , 55 ). (physiology.org)
  • A GFP reporter system to assess gene transfer and expression in human hematopoietic progenitor cells. (ebscohost.com)
  • Hematopoietic stem cells are widely recognized as attractive targets for gene therapy but current protocols to transduce these cells using recombinant retroviral vectors are inefficient. (ebscohost.com)
  • To evaluate optimization of retroviral transduction of hematopoietic stem cells and stability of gene. (ebscohost.com)
  • proliferate
  • In response to muscle injury or exercise, satellite cells are activated and proliferate and differentiate into mature fibers [ 3 ]. (hindawi.com)
  • They proliferate long term, retain a normal karyotype, are not tumorigenic and survive better under oxidative stress than CD56+ myogenic cells. (pitt.edu)
  • By imaging luciferase activity, real-time quantitative and kinetic analyses show that donor-derived muscle stem cells proliferate and engraft rapidly after injection until homeostasis is reached. (nih.gov)
  • vascular
  • Based on these differences, we have proposed that pre-invasive lobular tumors may progress to invasive lesions through " in situ malignant transformation ", in which the entire myoepithelial cell layer within a given lobule or lobular clusters undergoes extensive degeneration and disruptions, which allows the entire epithelial cell population associated with these myoepithelial cell layers directly invade the stroma or vascular structures. (jcancer.org)
  • In contrast, pre-invasive ductal tumors may invade the stroma or vascular structures through " progenitor-mediated cell budding ", in which focal myoepithelial cell degeneration-induced aberrant leukocyte infiltration causes focal disruptions in the tumor capsules, which selectively favor monoclonal proliferation of the overlying tumor stem cells or a biologically more aggressive cell clone. (jcancer.org)
  • Development of a tissue-engineered vascular graft combining a biodegradable scaffold, muscle-derived stem cells and a rotational vacuum seeding technique. (pitt.edu)
  • muscle stem cells
  • Our studies implicate that the loss of TRIM32 results in dysfunctional muscle stem cells which could contribute to the development of LGMD2H. (purdue.edu)
  • Highly efficient, functional engraftment of skeletal muscle stem cells in dystrophic muscles. (harvard.edu)
  • Currently, the in vitro manipulation and culture of these cells is still in its infancy, yet muscle stem cells may be the most promising route toward the therapy of muscle diseases such as muscular dystrophies. (frontiersin.org)
  • Consequently, it is not clear at which time point one should compare the properties of head mesodermal cells and head muscle stem cells. (frontiersin.org)
  • However, in a number of diseases including muscular dystrophies, cancer and HIV/Aids, the ability of muscle stem cells to repair muscle is compromised. (frontiersin.org)