• pointed out that Mage-D1 may participate in the proliferation of bone marrow mesenchymal stem cells (BMSCs) and the differentiation of odontoblasts through the NF-κB signalling pathway 21 . (researchsquare.com)
  • However, enhanced maintenance and proliferation of iPS-like cells occurred in the FBS medium. (bvsalud.org)
  • WAT is characterised by its capacity to adapt and expand in response to surplus energy through processes of adipocyte hypertrophy and/or recruitment and proliferation of precursor cells in combination with vascular and extracellular matrix remodelling. (springer.com)
  • Failure of mammalian cells to regulate their proliferation cycle leads to cancer. (nih.gov)
  • An aberrant miRNA expression could contribute to cancer development and progression [ 6 , 7 ] and could affect their target genes that are involved in many biological processes, such as cell differentiation, proliferation, apoptosis, metabolism, and development [ 8 ]. (hindawi.com)
  • AMs are partially depleted at the peak of viral-induced inflammation, followed by the regeneration of AM pools by AM proliferation and monocyte differentiation into new AMs (Li et al. (biomedcentral.com)
  • These cells are the original precursors of various cells in a tumor, whereas other intratumoral cells are limited in terms of their potential for proliferation and pluripotent differentiation. (biomedcentral.com)
  • PANX1 channel inhibition using probenecid (PBN) or carbenoxolone (CBX) reduced the proliferation of our panel of high-risk NB cell lines. (jcancer.org)
  • We show that expression of the Y10F PANX1 mutant, which cannot be phosphorylated on tyrosine 10 and acts in a dominant-negative manner, curtailed NB cell proliferation. (jcancer.org)
  • The high proliferation and differentiation capacity of fetal MSCs and the role of fetal pancreatic-derived MSCs (FPMSCs) in islet generation make them good candidates for diabetes treatment. (biomedcentral.com)
  • A side-by-side comparison was made to evaluate the effect of each serum on proliferation rate, cell cycle, senescence, multi-lineage differentiation capacity, immunophenotype, and tumorigenesis of FPMSCs. (biomedcentral.com)
  • Mesenchymal stem cells (MSCs) have received more attention due to their proliferation and differentiation capacity and low immunogenicity. (biomedcentral.com)
  • In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)
  • They are usually distinguished from progenitor cells, which cannot divide indefinitely, and precursor or blast cells, which are usually committed to differentiating into one cell type. (wikipedia.org)
  • they are vastly outnumbered by the progenitor cells and terminally differentiated cells that they differentiate into. (wikipedia.org)
  • Recently, stage-specific embryonic kidney progenitor tissue was used to regenerate nephrons. (tau.ac.il)
  • Through fluorescence-activated cell sorting, microarray analysis, in vitro differentiation assays, mixed lymphocyte reaction, and a model of ischemic kidney injury, this study sought to identify and characterize multipotent organ stem/progenitor cells in the adult kidney. (tau.ac.il)
  • We were interested in the developmental potential of single cardiac progenitor cell into cardiomyocytes, smooth muscle cells, and endothelial cells. (stanford.edu)
  • Recent Accomplishments - Starting in 2013, we incorporated Fluidigm-based multiplex single cell PCR equipment HD Biomark to analyze the differentiation of single cardiac progenitor cells in vitro and found that Nkx2.5 expression marks a subpopulation of committed endocardial precursor cells in the mouse heart (Li et al, Development 2015). (stanford.edu)
  • These cells subsequently give rise to neural stem and progenitor cells which undergo further differentiation to neurons and glia [2]. (liveconscience.com)
  • Intradermal adipocyte progenitor cells are activated toward the end of the telogen phase to proliferate and differentiate into mature adipocytes. (jcadonline.com)
  • At present it remains unclear whether FRCs originate from a common embryonic mesenchymal progenitor or AS 602801 (Bentamapimod) if different lineages of mesenchymal cells generate the FRC network. (technologybooksindustrialprojectreports.com)
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
  • Adult stem cells can be used to accelerate bone or tendon healing , and they can induce cartilage progenitor cells to produce a better matrix and repair cartilage damage . (thefutureofthings.com)
  • Errors arising in stem and progenitor cells have a much greater influence on the tissue in which they are found than errors arising in postmitotic differentiated cells. (biomedcentral.com)
  • Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
  • To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)
  • A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
  • In vivo, they eventually differentiate into all of the body's cell types (making them pluripotent). (wikipedia.org)
  • These were termed induced pluripotent stem cells (iPSCs). (wikipedia.org)
  • 1. Development of a ML/AI pipeline for the prediction of subtypes and differentiation stage of developing mouse cardiac cells from mesoderm to birth and from in vitro differentiation of human induced pluripotent stem cells (hiPSCs). (stanford.edu)
  • One of the challenges in studying early differentiation of human embryonic stem cells (hESCs) is being able to discriminate the initial differentiated cells from the original pluripotent stem cells and their committed progenies. (liveconscience.com)
  • Our results demonstrate that expression of the pluripotent factor Oct4 is usually progressively downregulated and is accompanied by the gradual upregulation of neural genes whereas the pluripotent factor Sox2 is usually consistently expressed at high levels indicating that these pluripotent factors may play different roles in the regulation of neural differentiation. (liveconscience.com)
  • Introduction The developmental processes of many organs and tissues in an embryo originate from the pluripotent cells of the inner cell mass (ICM) in the blastocyst. (liveconscience.com)
  • Induced pluripotent stem (iPS) cells are generated from somatic cells and can differentiate into various cell types. (bvsalud.org)
  • Since rodent models cannot recapitulate many of the human disease features, human induced pluripotent stem cells derived from Parkinson's patients have been used to generate brain organoids, greatly contributing to our understanding of the disease pathophysiology. (nature.com)
  • Relating to Lu AF21934 these sights, many so-called differentiated cells possess the capability for wide identification transitions, which raises the relevant question of exactly what does it mean to get a cell Lu AF21934 to become pluripotent. (cancercurehere.com)
  • This cells can go through differentiation to create both shoots and origins, and therefore it was recommended that callus cells are inside a pluripotent condition (Ikeuchi et al. (cancercurehere.com)
  • 2011). During wounding of tree barks, callus can be Lu AF21934 shaped from multiple vasculature-associated cells and may generate a number of fresh ones, suggesting it offers some pluripotent potential (Stobbe et al. (cancercurehere.com)
  • It's possible that one cell types, just like the pericycle, are primed and may quickly acquire pluripotency currently, while cells from additional tissues have to go through a competence acquisition stage before their pluripotent potential turns into apparent. (cancercurehere.com)
  • These cells are considered pluripotent . (thefutureofthings.com)
  • In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)
  • 3 Embrionic stem cells (ESCs) are derived from the inner cell mass of the blastocyst and form all cell types, derived from the three germ layers, and are therefore pluripotent. (bvsalud.org)
  • In addition, these cells are enriched for β1-integrin, are cytokeratin negative, and show minimal expression of surface markers that typically are found on bone marrow-derived mesenchymal stem cells. (tau.ac.il)
  • 6,7 Mesenchymal stem cells (MSCs) have also been isolated from other sources, such as bone marrow, 8 the placenta, 9 muscle, 10 or blood. (jcadonline.com)
  • Mesenchymal stem cells (MSCs) have been suggested as an appropriate source for diabetes cell-based therapies. (biomedcentral.com)
  • Mesenchymal stem cells (MSCs) are ASCs, and were first described in 1966 by Friedenstein et al. (bvsalud.org)
  • To pursue Aim1 we will use in situ hybridization, immunostaining, prospective isolation of candidate NC cells by FACS and in vitro differentiation analysis to determine the vitro fates of candidate human NC cells. (ca.gov)
  • However, the in vitro differentiation conditions for all potential human NC cell fates are unknown. (ca.gov)
  • But without the support of extra-embryonic stem cells that go on to form the placenta and yolk sac, development stalls. (bioworld.com)
  • Now, two groups of researchers, led by scientists at the Weizmann Institute in Israel and Cambridge University, U.K., have taken the in vitro development of whole mouse embryos further, by adding or inducing the differentiation of trophoblast cells and extra-embryonic endoderm stem cells. (bioworld.com)
  • The early mammalian embryo consists of the extra-embryonic cell layers-the trophoblast and a body of cells called the inner cell mass (ICM), which eventually become the embryo proper. (thefutureofthings.com)
  • Li et al, Development 2019) as well as the developing cardiac conduction system at embryonic day 16.5 of mouse development (Goodyer et al, Circulation Research 2019). (stanford.edu)
  • 2000). ECE in avian cardiac conduction system differentiation and Valnoctamide maturation. (biotech-angels.com)
  • In this study, we aimed to derive iPS cells from cat embryonic fibroblasts retrovirally transfected with mouse Oct3/4, Klf4, Sox2, and c-Myc. (bvsalud.org)
  • After transfection, embryonic fibroblasts were reseeded onto inactivated SNL 76/7 and cultured in a medium supplemented with basic fibroblast growth factor. (bvsalud.org)
  • In this setting, IPF is defined as a chronic progressive fibrotic ILD, with unknown aetiology and a pattern of usual interstitial pneumonia (UIP), which is represented by heterogeneous lesions at different stages of evolution with foci of proliferative fibroblasts. (ersjournals.com)
  • 2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)
  • 2011 Thus a full understanding of the ontogeny and function of the stromal microenvironment still requires that we uncover the genetic and transcriptional programs underlying mesenchymal cell differentiation and elucidate the molecular repertoire that characterize each stromal subsets during normal and pathological conditions. (technologybooksindustrialprojectreports.com)
  • We demonstrate here that GSK-3 maintains the MLL leukemia stem cell transcriptional program by promoting the conditional association of CREB and its coactivators TORC and CBP with homedomain protein MEIS1, a critical component of the MLL-subordinate program, which in turn facilitates HOX-mediated transcription and transformation. (stanford.edu)
  • In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)
  • In summary, these data reveal specific complexities in the transcriptional programming of antigen-specific ASC populations. (biotech-angels.com)
  • The aim of this thesis work is to interrogate the pro- and anti- tumorigenic roles myeloid cells play in primary and metastatic disease, and how these myeloid populations interact with cells of the adaptive immune system to influence anti-tumor immunity. (ubc.ca)
  • TIME possesses distinct populations of myeloid cells and lymphocytes to influence the immune escape of cancer, the response to immunotherapy, and the survival of patients. (biomedcentral.com)
  • Among the components of TME, distinct populations of innate and adaptive immune cells consist of tumor immune microenvironment (TIME). (biomedcentral.com)
  • Because of discrepancies in the expression of results and their application to populations with varying risk potentials, DNA ploidy and LOH assays cannot be directly compared. (biomedcentral.com)
  • Abstract Osteogenic differentiation of various adult stem cell populations such as neural crest-derived stem cells is of great interest in the context of bone regeneration. (uni-bielefeld.de)
  • Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
  • Yet another group of genes associated with cancer formation regulates programmed cell death, or apoptosis. (the-scientist.com)
  • The importance of apoptosis is exemplified by the vertebrate adaptive immune system, in which B lymphocytes, as they differentiate from their precursor cells, rearrange the DNA of immunoglobulin genes to generate a vast number of potential antibodies. (the-scientist.com)
  • Furthermore a previous report using stromal-feeder based neural differentiation protocol. (liveconscience.com)
  • Adipose tissue (AT) represents a commonly used source of mesenchymal stem/stromal cells (MSCs) whose proregenerative potential has been widely investigated in multiple clinical trials worldwide. (mdpi.com)
  • In addition to tumor cells, there are also important stromal components in tumor niche. (biomedcentral.com)
  • In this review, we discuss the potential influence of stromal vascular fraction from adipose tissue in the management of alopecia as well as its involvement in preclinical and clinical trials. (jcadonline.com)
  • however, a variety of other cell types are found as well, collectively labeled as stromal vascular fraction (SVF). (jcadonline.com)
  • But it is where researchers from Immatics SA and the University of Pennsylvania have found a target that was expressed on stromal cells in a number of different solid tumors, but very rare in normal tissues. (bioworld.com)
  • lymphoid tissue stromal organizer cells. (technologybooksindustrialprojectreports.com)
  • Additionally it is unidentified at what stage during lineage diversification mesenchymal cells become completely committed toward a specific fate and whether distinct stromal cell subsets arise from single multipotent progenitors or if different precursors exists for each stromal cell type. (technologybooksindustrialprojectreports.com)
  • Background: Stromal cells are a functionally important component of human carcinomas. (immune-source.com)
  • Western blot analysis showed that stromal cells of lung tumours were characterised by a statistically considerably lower phrase level of the p16 proteins as likened with that in regular lung stromal cells. (immune-source.com)
  • Stromal tumors of the ovary include germ-cell tumors, sex-cord stromal tumors, and other more rare types. (medscape.com)
  • abstract = "Tissue engineering and cell therapy approaches aim to take advantage of the repopulating ability and plasticity of multipotent stem cells to regenerate lost or diseased tissue. (tau.ac.il)
  • However, when they are isolated and cultured in vitro, they can be kept in the stem-cell stage and are known as embryonic stem cells (ESCs). (wikipedia.org)
  • Although differentiation of ESCs may not fully recapitulate the development of the embryo increasing evidence demonstrates that their lineage-specific differentiation nonetheless reflects the developmental progression of that cell type 9 days) (Physique 1B). (liveconscience.com)
  • 1,2 Regarding the origin, they can be classified as embryonic stem cells (ESCs) and adult stem cells (ASCs). (bvsalud.org)
  • The use of ASCs in regenerative medicine and tissue engineering research has important advantages in comparison with ESCs, since there are no ethical complications and the process of differentiation of these cells is better controlled. (bvsalud.org)
  • Astrocytes are the most abundant glial cells in the mammalian central nervous system and they serve essential functions in brain development and homoeostasis. (nature.com)
  • Research over the past decade has shown embryonic stem cells can undergo many disparate aspects of mammalian embryogenesis in vitro. (bioworld.com)
  • Our current research now focuses on two basic, interrelated questions: (1) How do mammalian cells decide where and when to initiate DNA replication? (nih.gov)
  • In the past, our research focused on viral genomes as models for DNA replication in mammalian cell nuclei. (nih.gov)
  • L-type Ca 2+ channels (LTCCs) drive the bulk of voltage-gated Ca 2+ entry in vertebrate inner ear hair cells (HCs) and are essential for mammalian auditory processing. (jneurosci.org)
  • Astrocytes have essential functions in brain homeostasis that are established late in differentiation, but the mechanisms underlying the functional maturation of astrocytes are not well understood. (nature.com)
  • Investigating astrocyte maturation in a cell culture model revealed that in vitro-differentiated astrocytes lack expression of many mature astrocyte-specific genes, including genes for the transcription factors Rorb, Dbx2, Lhx2 and Fezf2. (nature.com)
  • During a subsequent phase of maturation in the first few postnatal weeks, immature astrocytes exit the cell cycle and aquire a fully mature phenotype 1 . (nature.com)
  • To gain insights into the mechanisms driving astrocyte maturation, we have characterised acutely isolated murine astrocytes at both postnatal and adult stages, as well as in vitro differentiated astrocytes. (nature.com)
  • Transcription factors such as PPAR-γ, ERG2, and BACH2 helps in maturation and differentiation of fetal monocytes that seeds alveolar space during embryonic stage. (biomedcentral.com)
  • 11 However, the greater the abundance of adipose tissue in the human body, the significantly higher the yield of MSCs is compared to other tissues. (jcadonline.com)
  • Flow cytometry analysis and three-lineage differentiation ability demonstrated that fibroblast-like cells obtained from primary culture had MSCs' characteristics. (biomedcentral.com)
  • It has been suggested that tissue-matched MSCs may increase the efficacy of their regenerative effects [ 7 ]. (biomedcentral.com)
  • It is suggested that MSCs isolated from each tissue may be a more appropriate source for regenerating the same tissue. (biomedcentral.com)
  • MSCs can be isolated from different locations, such as bone marrow, umbilical cord, placenta, adipose and dental tissues. (bvsalud.org)
  • White adipose tissue (WAT) has key metabolic and endocrine functions and plays a role in regulating energy homeostasis and insulin sensitivity. (springer.com)
  • Conversely, brown adipose tissue (BAT) and browning of WAT represent potential therapeutic approaches, since dysfunctional white adipocyte-induced lipid overspill can be halted by BAT/browning-mediated oxidative anti-lipotoxic effects. (springer.com)
  • Here, we summarise the mechanisms contributing to adipose tissue (AT) plasticity and function including characteristics and cellular complexity of the various adipose depots and we discuss recent insights into AT origins, identification of adipose precursors, pathophysiological regulation of adipogenesis and its relation to WAT/BAT expandability in obesity and its associated comorbidities. (springer.com)
  • White adipose tissue (WAT) plays a key homeostatic role, not only by ensuring efficient energy storage but also by its quick mobilisation (lipids) to ensure peripheral demands. (springer.com)
  • The 'adipose tissue expandability model' identifies the limited capacity and dysfunctionality of WAT, preventing its expansion and accommodation of surplus of energy, as key determinants for the onset and progression of obesity-associated metabolopathologies as a result of ectopic deposition of toxic lipid species in metabolic organs (i.e. muscle or liver [also known as lipotoxic insult]) [ 7 ]. (springer.com)
  • There is also evidence that the deleterious effects mediated by dysfunctional white adipocyte-induced lipid overspill can be halted by the pro-oxidative anti-lipotoxic effects mediated by brown adipose tissue (BAT) activation. (springer.com)
  • Adipose cells organized in small clusters under the reticular dermis closely interact with hair follicular cells and regulate the hair cycle. (jcadonline.com)
  • Adipose tissue is a loose connective tissue composed of cells supported by an intracellular matrix as well as by vascular, lymphatic, and neural networks. (jcadonline.com)
  • Mechanisms of cellular motility are highly conserved and regulate many physiological processes including embryonic development, wound healing, and angiogenesis. (ubc.ca)
  • Our research focuses on developmental pathways that regulate hematopoietic cell growth and differentiation and are disrupted in the course of neoplastic transformation, particularly in leukemias and lymphomas. (stanford.edu)
  • Normal function is to regulate cell division. (the-scientist.com)
  • 2017, Z. around the emergence of patterns and tissue organization, and information regarding the events occurring at the level of individual cells is only now beginning to emerge. (cancercurehere.com)
  • 2017-Present Istinye University Stem Cell and Tissue Engineering RD Manager. (livhospital.com)
  • Further studies confirmed that p75NTR participates in the regulation of tooth development maybe by changing the activity of the key factor distal-less homeobox/msh homeobox (Dlx/Msx), and melanoma-associated antigen D1 (Mage-D1) seems to be play a role in the differentiation and mineralization of EMSCs 5 , 6 . (researchsquare.com)
  • Mage-D1, also known as Dlxin-1 or NRAGE, was first cloned and identified as a new member of the type II melanoma-associated antigen gene family by Pold et al. (researchsquare.com)
  • The process of isolating these cells has been controversial, because it typically results in the destruction of the embryo. (wikipedia.org)
  • Somatic cell nuclear transfer is a cloning method that can be used to create a cloned embryo for the use of its embryonic stem cells in stem cell therapy. (wikipedia.org)
  • In Aim2 we will determine the in vivo fates of candidate human NC cells by transplanting genetically labeled hES-NPCs and their subpopulations into the early chick embryo and following their fates during chick development. (ca.gov)
  • Therefore they provide not only a potential source of specialized cells for regenerative therapies but also a valuable model to study early human development particularly as the direct study of early human embryo development is usually severely hampered by the inability to obtain adequate amounts of tissues at all developmental stages. (liveconscience.com)
  • 3,4 The zygote and cells derived from the first two cellular divisions constitute the most primitive cells (totipotent cells) that are capable of forming the embryo and the embryonic annexes (e.g. placenta, amniotic membranes etc). (bvsalud.org)
  • This process starts with the differentiation into the three germ layers - the ectoderm, mesoderm and endoderm - at the gastrulation stage. (wikipedia.org)
  • The ICM continues to differentiate into three germ layers-ectoderm, mesoderm and endoderm, each of which follows a specific developmental destiny that takes them along an ever-specifying path at which end the daughter cells will make up the different organs of the human body. (thefutureofthings.com)
  • Since 1998 however, it has been possible to culture and differentiate human embryonic stem cells (in stem-cell lines). (wikipedia.org)
  • By 1998, human embryonic stem cells were first isolated by American biologist James Thomson, which made it possible to have new transplantation methods or various cell types for testing new treatments. (wikipedia.org)
  • We also use human stem cells as a test-tube model to study the process of heart formation and to create new human heart tissues and organs from these stem cells. (stanford.edu)
  • Abnormalities in NC cells involved in numerous human pathologies including various skeletal syndromes (e.g. (ca.gov)
  • Human NC cells have never been isolated and characterized on cellular and molecular levels. (ca.gov)
  • The PI laboratory has recently developed an efficient procedure for the rapid differentiation of human ES cells into uniform neural precursors (hES-NPCs), which was hitherto unachievable. (ca.gov)
  • Our hypothesis is that human NC cells are present in hES-NPCs, established in our laboratory. (ca.gov)
  • If our hypothesis is correct, hES cell-derived neural precursors will be a rich source for neural crest cells, thus allowing for the first time the extensive characterization of these rare human cells and the development of strategies of NC cell-based protocols in clinics. (ca.gov)
  • If our hypothesis is correct, hES cell-derived neural precursors will be a rich source for NC cells, and subsequently for an array of human NC derivatives such as bones, cartilage, muscles, peripheral and enteric neurons and melanocytes. (ca.gov)
  • Human embryonic stem cells (hESCs) derived from the ICM of blastocysts are capable of self-renewal in culture indefinitely and meanwhile retain the developmental pluripotency of the embryonic founder cells having the potential to differentiate into all the cells and tissues in a human body [3]. (liveconscience.com)
  • Renal cell carcinoma (RCC) accounts for approximately 3% of human malignancies, and its incidence appears to be increasing globally [ 1 ]. (hindawi.com)
  • In a comparative analysis between the human microRNA datasets and the chimpanzee, gorilla, orangutan, macaque, mouse, fruit fly, and mouse genomes, Dr. Rigoutsos and colleagues discovered that almost 95% of the newly unveiled microRNAs were primate-specific, and over 56% of them were found only in humans. (pharmaceuticalintelligence.com)
  • In rodents, and even in some preliminary trials in humans, human embryonic stem cells have been shown to bridge gaps in spinal cord injuries , allowing restoration of motor functions. (thefutureofthings.com)
  • To elucidate the osteoinductive potential of porous substrates with different pore diameters (30 nm, 100 nm), human neural crest-derived stem cells isolated from the inferior nasal turbinate were cultivated on the surface of nanoporous titanium covered membranes without additional chemical or biological osteoinductive cues. (uni-bielefeld.de)
  • Cultivation of human neural crest-derived stem cells on 30 nm pores resulted in osteogenic differentiation as demonstrated by alkaline phosphatase activity after seven days as well as by calcium deposition after 3 weeks of cultivation. (uni-bielefeld.de)
  • In our recent study, we examined the impact of the three-dimensional co-culture of FPMSCs on human embryonic stem cell-derived pancreatic progenitors' development. (biomedcentral.com)
  • Extracellular vesicle DNA from human melanoma tissues contains cancer-specific mutations. (gu.se)
  • with four transmembrane domains that are indicated in human cells. (biotech-angels.com)
  • 8,9 Because dental stem cells (DSCs) are easy to obtain and present a great potential of differentiation, there has been a growing interest in their use in regenerative medicine for treatment of various human diseases. (bvsalud.org)
  • In human postnatal dental tissues, five main sources of DSCs have been identified: dental pulp stem cells (DPSCs), 11 stem cells from human exfoliated deciduous teeth (SHEDs), 12 periodontal ligament stem cells (PDLSCs), 13 dental follicle stem cells (DFSCs) 14 and stem cells from apical papilla (SCAPs). (bvsalud.org)
  • A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. (worthington-biochem.com)
  • Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics. (worthington-biochem.com)
  • 1999. Transactivation activity of human, zebrafish, and rainbow trout aryl hydrocarbon receptors expressed in COS-7 cells: Greater insight into species differences in toxic potency of polychlorinated dibenzo- p -dioxin, dibenzofuran, and biphenyl congeners. (cdc.gov)
  • Neurologic dysfunction probably results from an autoimmune reaction directed against onconeural antigens in the human nervous system. (medscape.com)
  • DBS is a widely used FDA-approved treatment and stem cell-derived dopamine neuron replacement has now evolved to the first in-human clinical trials. (lu.se)
  • In Chapter 2, I examined how proteins secreted by cancer cells can promote a tumor-permissive microenvironment through the recruitment of suppressive innate and adaptive immune cells to the lungs. (ubc.ca)
  • I demonstrated that tumor cells secreted proteins that drove the recruitment and/or expansion of pulmonary TAMs, Tregs, and granulocytic-MDSCs (G-MDSCs), resulting in increased primary lung tumor growth and pulmonary metastasis of mammary carcinoma cells, respectively. (ubc.ca)
  • Targeting tumor-secreted proteins, or the suppressive immune cells that are expanded in response to said proteins, led to a decrease in primary and metastatic pulmonary disease. (ubc.ca)
  • This study aimed to evaluate the expression levels of miR-34a and 11 of its bioinformatically selected target genes and proteins to test their potential dysregulation in RCC. (hindawi.com)
  • The normal function of the proteins expressed by these genes is to recognize superfluous, damaged, aged, or aberrant cells that must be eliminated. (the-scientist.com)
  • As part of its charge, the committee was asked to prepare a subreport evaluating methods for detecting potential unintended compositional changes across the spectrum of messenger ribonucleic acid (mRNA), proteins, metabolites and nutrients that may occur in food derived from cloned animals that have not been genetically modified via genetic engineering methods. (nationalacademies.org)
  • The neuronal proteins are homologous to the embryonic lethal abnormal visual (ELAV) protein in Drosophila species. (medscape.com)
  • These cellular components can include cells of the innate immune system, such as myeloid-derived suppressor cells (MDSCs), tumor-associated macrophages (TAMs), and eosinophils, as well as cells of the adaptive immune system, including CD4⁺ T regulatory cells (Tregs) and cytotoxic CD8⁺ T cells. (ubc.ca)
  • In Chapter 4, I profiled the dynamic myeloid compartment following antigen specific CD8⁺ T cell-mediated tumor regression and recurrence. (ubc.ca)
  • I demonstrated that using glucose restriction of CD8⁺ T cells in vitro led to improved tumor regression in vivo. (ubc.ca)
  • I identified several potentially suppressive myeloid cell subsets, including TAMs and arginase-1ʰⁱ monocytic-MDSCs (M-MDSCs) that expand during CD8⁺ T cell-mediated tumor regression and subsequent recurrence, which may play a role in tumor regrowth. (ubc.ca)
  • This thesis work aims to examine the diverse phenotype and function of myeloid cells in primary and metastatic disease, ultimately contributing to an improved understanding of the interplay between the innate and adaptive immune system in the solid tumor microenvironment. (ubc.ca)
  • This study aimed to activate the antitumor immune response by manipulating the TME by transfecting genes encoding relevant cytokines into tumor cells using a synthetic vehicle, which is designed to target tumor cells and promote the expression of transfected genes. (bvsalud.org)
  • Upon intravenous injection of the green fluorescent protein-coding plasmid encapsulated in the vehicle, 14.2% tumor-specific expression was observed. (bvsalud.org)
  • As a result, tumor cell-specific transfection of these cytokine genes by the synthetic vehicle significantly promotes antitumor immune responses in the TME, a key aim for visceral tumor therapy. (bvsalud.org)
  • and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)
  • Abnormal cell division is a hallmark of tumor cells. (the-scientist.com)
  • According to the hypothesis, only a small group of stem cell-like tumor cells can form new tumors. (biomedcentral.com)
  • In addition, normal adult stem cells (ASCs) self-renew, proliferate infinitely, have a multidirectional differentiation potential, similar to that of tumor cells, and can transform more easily into tumor stem cells than non-stem cells [ 20 , 21 ]. (biomedcentral.com)
  • and 5) DNA index or tumor cell ploidy [ 4 , 5 ]. (jcancer.org)
  • Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. (worthington-biochem.com)
  • TME consists of cell component, the extracellular matrix (ECM) and abundant soluble signaling molecules. (biomedcentral.com)
  • These cells, surrounding the hair follicles, secrete signaling molecules that control the progression of the hair cycle. (jcadonline.com)
  • 2001 thus indicating that different signaling molecules and cell types may be required for proper FRC differentiation in different lymphoid organs. (technologybooksindustrialprojectreports.com)
  • showed that knocking out Mage-D1 can induce the expression of autophagy-related genes by enhancing the activity and differentiation of osteoclasts, thereby accelerating the process of periodontitis 23 . (researchsquare.com)
  • Despite focusing on functional studies of correlatively pathogenic genes, less research has been done to investigate possibilities of their counter-regulatory genes as potential modulators of brain morphogenesis and therapeutic targets of malformation. (frontiersin.org)
  • Whether miR-7, one of the microcephaly-pathogenic genes, could act as a potential modulator to remedy macrocephaly remains obscure. (frontiersin.org)
  • This mechanism also applies to hematopoietic cells transformed by other HOX genes, including CDX2, which is highly expressed in a majority of acute myeloid leukemias, thus providing a molecular approach based on GSK-3 inhibitory strategies to target HOX-associated transcription in a broad spectrum of leukemias. (stanford.edu)
  • In fact, amplification of genes by over replication of certain regions of DNA is one of the primary mechanisms by which cancer cells become resistant to drug therapy. (nih.gov)
  • explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)
  • In conclusion, this is the first study to establish and characterize cat iPS-like cells, which can differentiate into different cell types depending on the basic fibroblast growth factor. (bvsalud.org)
  • These adult stem cells are considered multipotent, having the ability to differentiate into different cell types, albeit with a more limited repertoire than embryonic stem cells. (thefutureofthings.com)
  • This is largely due to the lack of adequate tissues from the early developmental stages. (liveconscience.com)
  • In mammals, roughly 50-150 cells make up the inner cell mass during the blastocyst stage of embryonic development, around days 5-14. (wikipedia.org)
  • ECM is a macromolecular substance secreted by cells into the extracellular space and constitutes a complex network that supports tissue structure and the physiological activities of cells, including collagen, elastin fibrils, proteinases, proteoglycans (PGs), glycoproteins and glycosaminoglycans (GAGs). (biomedcentral.com)
  • Dendritic cells-based therapeutic approach may be a potential strategy for SARS-CoV-2 infection. (medsci.org)
  • Basis of treatment based dendritic cells to combat coronavirus infections is summarized. (medsci.org)
  • Immune status analysis revealed that the density of dendritic cells significantly increased in tumors, particularly after GM-CSF- and CD40L-gene transfection, while that of regulatory T cells significantly decreased. (bvsalud.org)
  • and the innate immune cells contain natural killer (NK) cells and dendritic cells (DCs) (Fig. 1 ). (biomedcentral.com)
  • Dendritic cell as sentinel of the immune system plays an irreplaceable role. (medsci.org)
  • CITE-seq) for immune cell phenotyping, and multiplexing samples from different sources using custom-made antibodies or lipid binding dye (i.e. (stanford.edu)
  • Recently, we have begun to address the pathogenic immune response in patients with myocarditis from treatment with immune checkpoint inhibitors (ICIs) for cancer therapy using single cell immunology tools from 10x Genomics. (stanford.edu)
  • Cancer is not a chaotic malignant cell mass, but a delicate "hostile" organ, where many other cells are recruited and domesticated to become "accomplices", thereby protecting themselves from recognition and attack by the immune system [ 1 ]. (biomedcentral.com)
  • TIME primarily consists of myeloid cells, lymphocytes and some other innate immune cells. (biomedcentral.com)
  • Substantial cells are divided into immune cells and non-immune cells. (biomedcentral.com)
  • The infiltrating immune cells in the TME constitute the main body of TIME. (biomedcentral.com)
  • Supplementary lymphoid tissues such as lymph nodes are essential for the interactions between antigen presenting cells and lymphocytes that result in adaptive immune responses that protect the host against invading pathogens. (technologybooksindustrialprojectreports.com)
  • To understand the multifaced aspects of Parkinson's disease, it may be desirable to expand the complexity of these models, to include different brain regions, vasculature, immune cells as well as additional diverse organ-specific organoids such as gut and intestine. (nature.com)
  • But nonviral tumors, which are composed of aberrant host cells, do not provide such targets, and the immune response is suppressed by defenses against autoimmune reactions. (the-scientist.com)
  • While tissue-resident macrophages act as sentinels of the alveolar space and play an important role in maintaining homeostasis and immune regulation, recruited macrophages accumulate in the respiratory tract after acute viral infections. (biomedcentral.com)
  • 2021 ). Not only are macrophages among the first immune cells to encounter viral particles in the lung, but they also maintain lung function by engulfing small debris and regulating surfactant turnover (Aegerter et al. (biomedcentral.com)
  • Although the aetiology of these disorders remains unknown, in this review we analyse the pathogenic mechanisms by cell of interest (fibroblast, fibrocyte, myofibroblast, endothelial and alveolar epithelial cells and immune competent cells). (ersjournals.com)
  • They are the earliest type of cell in a cell lineage. (wikipedia.org)
  • This population of small cells includes a CD45-negative fraction that lacks hematopoietic stem cell and lineage markers and resides in the renal interstitial space. (tau.ac.il)
  • It is widely accepted that the cell origin for NB arises from the sympathoadrenal lineage of the neural crest during development [ 3 ]. (jcancer.org)
  • The nature of the stem cell substates and their relationship to commitment to differ- entiate and lineage selection can be elucidated in terms of a landscape picture in which stable states can be defined mathematically as attractors. (lu.se)
  • This requirement strongly limits the number of solutions or entiation and lineage-specification, programmed cell death, and ``states'' for the system. (lu.se)
  • Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
  • We are studying the role that normal chromatin structure plays in gene regulation in hematopoietic cells and how its disruption leads to altered development and cancer. (stanford.edu)
  • The induction from the AP2-like transcription element gene causes the creation of callus from epidermal cells (Iwase et al. (cancercurehere.com)
  • It has been found that some of the SVF cell types possess regenerative and anti-inflammatory potentials in damaged tissues due to their ability to secrete growth factors and anti-inflammatory molecules. (jcadonline.com)
  • As a result, SVF has attracted substantial attention for its potential use in regenerative medicine in various fields, including internal medicine, 13-15 orthopedics, 16 plastic and general surgery, 17-19 and wound healing. (jcadonline.com)
  • In order to address the shortcomings related to current treatment modalities, many groups have diverted their attention to utilizing tissue engineering and regenerative medicine approaches. (biomedcentral.com)
  • In order to address the limits associated with current therapies, many research groups have investigated various regenerative medicine strategies utilizing different stem cell sources to engineer artificial salivary tissues that can mitigate the effects of xerostomia and hyposalivation. (biomedcentral.com)
  • Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
  • 2014-Present Professor Liv Hospital (Ulus) Regenerative Medicine Stem Cell Production Center Istanbul- Turkey. (livhospital.com)
  • Conclusion: stem cells from dental origin are an interesting alternative for research and application in regenerative therapies in Dentistry. (bvsalud.org)
  • For example the development of the central nervous system is initiated following gastrulation by the induction of the neuroectoderm a process by which embryonic cells acquire a neural fate to form a single layer of neuroepithelial cells [1]. (liveconscience.com)
  • This multi-step cell fate determination that occurs during embryonic neurogenesis is usually delicately orchestrated by ARQ 197 many signalling pathways and transcription factors. (liveconscience.com)
  • The cells of the ICM are no longer omnipotent, because they no longer share the fate of the trophoblast, and they have committed themselves to an embryonic fate with the ability to become any cell in the body (but not the trophoblast). (thefutureofthings.com)
  • Here we show that cortical deletion of Gli3 results in enlarged brain and folding structures in the cortical midline at the postnatal stage, which is mainly caused by the increased percentage of intermediate progenitors (IPs) and newborn neurons. (frontiersin.org)
  • Specific properties, such as proliferative and differentiation potential, are slightly different among DSCs. (bvsalud.org)
  • Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
  • although it is derived from cells of the renal tubular epithelium, it has several histological subtypes which differ in their clinical outcome and biological features. (hindawi.com)
  • We will specifically assay the subsets of cells using specific NC-associated markers to enrich for candidate NC lineages. (ca.gov)
  • New insights into the complex cellular contributions and interactions will be provided, comparing the role of cell subsets in the pathogenesis of IPF and systemic sclerosis. (ersjournals.com)
  • As of 2016[update], the only established medical therapy using stem cells is hematopoietic stem cell transplantation, first performed in 1958 by French oncologist Georges Mathé. (wikipedia.org)
  • in first stages of epidermis differentiation offers detected stochastic manifestation of the transcription element that didn't always match morphological identification transitions (Costa 2016). (cancercurehere.com)
  • 2016). Nevertheless, transcriptome-level data of cell identification transitions are scant still, and the type of the hypothetical transition condition remains to become elucidated. (cancercurehere.com)
  • The purpose of this study was to investigate the expression pattern of Mage-D1 in rat tooth germ development and its differential mineralization ability to ectomesenchymal stem cells (EMSCs), and to explore its potential mechanism. (researchsquare.com)
  • Results showed that the expression of Mage-D1 during rat tooth germ development was temporally and spatially specific. (researchsquare.com)
  • These iPS-like cells expressed embryonic stem cell markers, had normal karyotypes, proliferated beyond 45 passages, and differentiated into all three germ layers in vitro. (bvsalud.org)
  • We subsequently became interested in developing a developing heart expression atlas in order to identify the cell type, developmental stage, and anatomical location of each single cardiac cells during mouse heart development using a random forest-based computational algorithm that we termed ATLAS-seq. (stanford.edu)
  • Recent studies have shown that Mage-D1 is closely related to tooth development, but its specific regulatory mechanism is unclear. (researchsquare.com)
  • Ectomesenchymal stem cells (EMSCs) derived from the cranial neural crest are significant in tooth development and dental mineralization. (researchsquare.com)
  • The development of teeth is initiated by epithelial-mesenchymal interactions, which form dental papilla cells and dental sac cells, subsequently forming pulp, dentin, cementum, periodontal ligament and proper alveolar bone, except enamel 1 - 4 . (researchsquare.com)
  • As development ARQ 197 proceeds these cells gradually acquire specialized traits becoming committed to specific fates and losing their potential to differentiate into other cell types. (liveconscience.com)
  • Tissue engineering is defined as the application of life sciences and materials engineering toward the development of tissue substitutes that are capable of mimicking the structure and function of their natural analogues within the body. (biomedcentral.com)
  • The general underlying strategy behind the development of tissue engineered organ substitutes is the utilization of a combination of cells, biomaterials, and biochemical cues intended to recreate the natural organ environment. (biomedcentral.com)
  • My laboratory has developed new technologies and applied them towards understanding the molecular biology and enzymology of DNA replication in animal cells and viruses (SV40, polyomavirus, papillomavirus, and herpes simplex virus), and at the beginning of animal development (mouse preimplantation embryos and frog eggs). (nih.gov)
  • These fresh views of cell differentiation and identity are undergoing rapid development and so are more likely to change. (cancercurehere.com)
  • Increases in the amount of PAPP-A mRNA in granulosa cells during follicular development occurs in some but not all species, indicating that other proteases or protease inhibitors may be involved in IGFBP degradation. (bioone.org)
  • When evaluating with microsatellite steady tumors, there is normally some proof for Kitty least partialC immunological development control in MSI malignancies, like (I) the thick regional lymphocytic infiltration (Crohn's-like lymphocytic response), (II) the elevated apoptotic growth cell amount, and (3) the low amount of isolated metastases that (4) network marketing leads to an improved general individual success [1]C[3]. (immune-source.com)
  • This remark is certainly a stunning point in favor of a significant Refametinib contribution to growth development control by FSP-specific Testosterone levels cells in vivo , producing those peptides extremely interesting applicants for the advancement of targeted vaccination strategies. (immune-source.com)
  • These cells have been studied for the treatment of periodontitis, bone repair, regeneration of the pulp after necrosis as well as the development of new teeth. (bvsalud.org)
  • As a graduate student with Julius Adler, I identified the basal body of the bacterial flagellum, develop methods for its purification, and elucidate its fine structure and specific attachments to the bacterial cell envelope. (nih.gov)
  • Intriguingly, our preliminary data show that many markers associated with NC cells are upregulated in hES-NPCs or during their derivation process. (ca.gov)
  • Our analysis will unequivocally determine the in vitro and in vivo fates of hES cell-derived cells with the neural crest cell markers present in our cultures. (ca.gov)
  • Basic patterns of hormones & early embryonic stages There have been few markers for ovulation & the first 10 post-ovulatory days. (slideserve.com)
  • Can precancerous stem cells be risk markers for malignant transformation in the oral mucosa? (biomedcentral.com)
  • Cancer cells can exploit normal mechanisms of cellular motility to facilitate invasion and metastasis, the process by which cancer cells disseminate to distant organs. (ubc.ca)
  • In addition these CD86 findings also showed that PDGFRβ+ perivascular cells from non-lymphoid organs have the capacity to differentiate into FDCs and in vivo thus suggesting that this cell population may be the source of AS 602801 (Bentamapimod) FDC in tertiary lymphoid organ formation (Krautler et al. (technologybooksindustrialprojectreports.com)
  • Central to these results is the idea that connections between lymphoid and mesenchymal cells are necessary for the introduction of supplementary lymphoid organs. (technologybooksindustrialprojectreports.com)
  • Due to the ability to self-renew and to differentiate into cells that are found throughout the body, there is a great interest in using stem cells for the regeneration of injured tissues as well as to develop tissue-engineered implants and bio-hybrid organs, in order to restore tissue function. (bvsalud.org)
  • Teeth, composed of three mineralized tissues (dentine, cementum, and enamel), constitute important models for gaining insight into the general processes of biological mineralization. (researchsquare.com)
  • Pulmonary macrophages, such as tissue-resident alveolar and interstitial macrophages and recruited monocyte-derived macrophages, are the major macrophages present in the lungs during homeostasis and diseased conditions. (biomedcentral.com)
  • As callus was considered to occur from mature cells, it had been assumed that cells must dedifferentiate if they type callus to be able to acquire pluripotency. (cancercurehere.com)
  • 2010). In this full case, no such pluripotency acquisition, or dedifferentiation, stage is necessary as these specialised cells may currently be in an extremely competent condition (Sugimoto et al. (cancercurehere.com)
  • 1958). This means that that as the pericycle, using its putative specific properties, may be the primary contributor to cells culture-based regeneration, pluripotency could be wide-spread amongst vegetable cells. (cancercurehere.com)
  • Notably, expression of exogenous Oct3/4, Klf4, and Sox2 was silenced in these cells. (bvsalud.org)
  • Ideally, exogenous differentiation should mimic an endogenous differentiation process, which is partly mediated by topological cues. (uni-bielefeld.de)
  • Both cell-derived microstructures and extracellular matrix components play critical roles in the preorganization and oriented deposition of calcium phosphate and serve as passive supports in dentine and enamel. (researchsquare.com)
  • During normal cell migration, cells use focal adhesions to facilitate cycles of cell adhesion to, and release from, the extracellular matrix (ECM). (ubc.ca)
  • Thus, it is urgent to develop therapeutic strategies to face the SARS-CoV-2 viral infection at the severe stage. (medsci.org)
  • With the ban on sub-therapeutic antibiotic usage in Europe and the increasingly strictness of the European legislation on food hygiene, passive immunization by oral administration of pathogen-specific hen egg yolk antibody (IgY) may be a useful and attractive alternative. (ac.be)
  • In the presence of bacterial toxins, it is involved in the cytokine storm and inflammatory signaling in monocytes and macrophages, becoming a potential therapeutic target against the infection. (bioworld.com)
  • Because miRNAs are easy to synthesize and deliver, miR-7 could be a potential therapeutic means to macrocephaly caused by Gli3-deficiency. (frontiersin.org)
  • Recently, the potential therapeutic use of miRNAs has been evaluated due to their dynamic and reversible properties. (hindawi.com)
  • A number of large biotech companies and scientists are looking toward stem cells as the basis for a therapeutic solution to cure such illnesses as blindness, diabetes and spinal cord injuries. (thefutureofthings.com)