- The genetic and protein experiments proved the obtention of DKCs that passed through the chronological stages of embryonic kidney development. (biomedcentral.com)
- Our results indicated that we obtained DKCs from mPSCs, which passed through the chronological stages of embryonic kidney development. (biomedcentral.com)
- WD develops in the early stages of embryonic development, and it has a pivotal role in the genitourinary system formation. (esur2023.com)
- Strategies include the rearrangement of pre-existing tissue, the use of adult somatic stem cells and the dedifferentiation and/or transdifferentiation of cells, and more than one mode can operate in different tissues of the same animal. (wikipedia.org)
- The mutation of MET plays a crucial role in the initiation of cancer, while the Hedgehog (Hh) pathway also plays a significant role in cell differentiation and the maintenance of tumor stem cells. (bvsalud.org)
- Conventional chemotherapy drugs are primarily designed to target the majority of cell populations within tumors rather than tumor stem cells. (bvsalud.org)
- Skeletal muscle stem cells (MuSC) are crucial for tissue homoeostasis and repair after injury. (bvsalud.org)
- therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells. (biomedcentral.com)
- In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. (biomedcentral.com)
- Consequently, because of the increased rate of RF in terminal stage and few alternative treatments, new investigations focused on regenerative medicine have been developed, including the use of stem cells (SCs) [ 7 ]. (biomedcentral.com)
- According to their potentiality, embryonic stem cells (ESCs) are classified as totipotent and pluripotent, both of them can differentiate into the three germ lines. (biomedcentral.com)
- Unidirectional regeneration in the basal chordate Ciona intestinalis involves the proliferation of adult stem cells residing in the branchial sac vasculature and the migration of progenitor cells to the site of distal injury. (biomedcentral.com)
- However, after the Ciona body is bisected, regeneration occurs in the proximal but not in the distal fragments, even if the latter include a part of the branchial sac with stem cells. (biomedcentral.com)
- The upregulation of HSP70 chaperone genes was verified and their expression confirmed in BS vasculature cells previously identified as stem and progenitor cells. (biomedcentral.com)
- This study demonstrates the importance of a stress response for stem cell activation and regeneration in a basal chordate, which may have implications for understanding the limited regenerative activities in other animals, including vertebrates. (biomedcentral.com)
- In biology, regeneration is the process of renewal, restoration, and tissue growth that makes genomes, cells, organisms, and ecosystems resilient to natural fluctuations or events that cause disturbance or damage. (wikipedia.org)
- At its most elementary level, regeneration is mediated by the molecular processes of gene regulation and involves the cellular processes of cell proliferation, morphogenesis and cell differentiation. (wikipedia.org)
- Cells in the primordia of zebrafish fins, for example, express four genes from the homeobox msx family during development and regeneration. (wikipedia.org)
- Dedifferentiation of cells means that they lose their tissue-specific characteristics as tissues remodel during the regeneration process. (wikipedia.org)
- This should not be confused with the transdifferentiation of cells which is when they lose their tissue-specific characteristics during the regeneration process, and then re-differentiate to a different kind of cell. (wikipedia.org)
- In recent years, interest in the possible molecular regulators of cell proliferation and differentiation in a wide range of regeneration models has grown significantly, but the cell kinetics of this process remain largely a mystery. (mdpi.com)
- It is well-established that early embryonic signaling pathways are critical for growth and patterning of new tissue during regeneration. (frontiersin.org)
- The regeneration of a tissue intuitively recapitulates aspects of its embryonic development. (frontiersin.org)
- Epimorphic regeneration is the process by which complete regeneration of a complex structure such as a limb occurs through production of a proliferating blastema. (biomedcentral.com)
- Furthermore, the underlying mesenchyme remains rounded and does not expand to form a cone shaped blastema, a normal feature of successful regeneration. (biomedcentral.com)
- Gene Ontology analysis showed that genes involved in embryonic development and growth are significantly over-represented in regenerating early hindlimb buds and that successful regeneration in the Xenopus hindlimb correlates with the induction of stress response pathways. (biomedcentral.com)
- Epimorphic regeneration can be thought of as occurring in two phases: wound healing and cell proliferation. (biomedcentral.com)
- We have developed a heat shock inducible transgenic line ( N1 ) of Xenopus in which the BMP antagonist Noggin can be induced at a specific time during either development or regeneration, repressing BMP signalling [ 10 , 12 ]. (biomedcentral.com)
- We have used this line to show that BMP function is not only required for appendage regeneration but that it is specifically needed to generate a proliferating blastema while being dispensable for wound healing [ 12 ]. (biomedcentral.com)
- Histological studies focusing on the early cephalic tentacle regeneration in P. canaliculata, have demonstrated that wound closure and blastema formation took place within 24 h post amputation (hpa). (unimore.it)
- siRNA-mediated gene knockdown showed that hsp70 and dnaJb4, but not bag3, are required for progenitor cell targeting and distal regeneration. (biomedcentral.com)
- Heat shock treatment of distal body fragments activated hsp70 and dnaJb4 expression indicative of a stress response, induced cell proliferation in branchial sac vasculature cells, and promoted distal regeneration. (biomedcentral.com)
- The stress response is absent from distal fragments, but can be induced by a heat shock, which activates cell division in the branchial sac vasculature and promotes distal regeneration. (biomedcentral.com)
- Using Affymetrix Gene Chip analysis, we have identified genes linked to regenerative success downstream of BMP signalling, including the BMP inhibitor Gremlin and the stress protein Hsp60 ( no blastema in zebrafish). (biomedcentral.com)
- Amputation-induced proliferation occurred predominantly within the epidermal and intestinal epithelium, as well as wound-adjacent muscle fibers, where clusters of cells at the same stage of the cell cycle were found. (mdpi.com)
- N1 transgenic hindlimbs, which do not regenerate, do not form an apical epithelial cap or cone shaped blastema following amputation. (biomedcentral.com)
- After amputation, granular hemocytes infiltrate the blastema of regenerating cephalic tentacles of the freshwater snail Pomacea canaliculata. (unimore.it)
- Once the wound is healed, the cells of the stump must mobilise under the wound epidermis and begin the process of replacing lost tissues, by forming a proliferating blastema. (biomedcentral.com)
- Advances in tissue engineering technology have led to the production of novel human skin equivalents and organoids that reproduce cell-cell interactions with tissue-scale tensional homeostasis, and enable us to evaluate skin tissue morphology, functionality, drug response and wound healing. (mdpi.com)
- Embryonic or adult SCs by definition are characterized by their self-renewal and potentiality, peculiarities that allow them to give rise to more SCs and differentiate into various cell lineages under appropriate conditions. (biomedcentral.com)
- The metanephric blastema forms glomeruli, proximal and distal tubules. (esur2023.com)
- 873 During the developmental process, genes are activated that serve to modify the properties of cell as they differentiate into different tissues. (wikipedia.org)
- After the limb or tail has been autotomized, cells move into action and the tissues will regenerate. (wikipedia.org)
- Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)
- In the presence of a Y chromosome and the SRY gene, müllerian‐inhibiting substance produced by Sertoli cells causes regression of the MD. Meanwhile, testosterone produced by the Leydig cells induces differentiation of the WD into the efferent ducts that communicate with the rete testis, followed by the epididymis, ductus deferens, seminal vesicle, and ejaculatory duct. (esur2023.com)
- Neural cells, for example, express growth-associated proteins, such as GAP-43, tubulin, actin, an array of novel neuropeptides, and cytokines that induce a cellular physiological response to regenerate from the damage. (wikipedia.org)
- mitotically active cells of intact segments do not significantly contribute to the blastemal cellular sources. (mdpi.com)
- In both processes, rapid proliferation gives rise to new tissue, cell fate has to be specified within that tissue, and distinct positional identities have to be established to generate a properly patterned structure. (frontiersin.org)
- The ureteric bud grows laterally and invades the center of the metanephric blastema, the primordial renal tissue [4]. (esur2023.com)
- In males, the establishment of internal reproductive system involves two key events: the formation of the testis and the maintenance and differentiation of the WD. (esur2023.com)
- Pattern formation in the morphogenesis of an animal is regulated by genetic induction factors that put cells to work after damage has occurred. (wikipedia.org)
- Although not essential for initial WD formation, the later differentiation of this embryological structure relies on androgens derived from the Leydig cells. (esur2023.com)
- ESCs are obtained from inner cell mass, characterized by unlimited self-renewal and pluripotentiality, which allow them to differentiate into various specialized cells types in a morphologically and functionally way, characteristics that make them attractive for developing differentiated kidney cells (DKCs) [ 9 ]. (biomedcentral.com)
- Finally, the principal components analysis obtained from FTIR spectra allowed to characterize chemical and structurally mPSCs and their differentiation process to DKCs in a rapid and non-invasive way. (biomedcentral.com)
- Moreover, FTIR spectroscopy resulted in a non-invasive, rapid and precise technic that together with principal component analysis allows to characterize chemical and structurally both kind of cells and also discriminate and determine different stages along the cell differentiation process. (biomedcentral.com)
- After the injection of clodronate liposomes, a specific computer-assisted image analysis protocol still evidenced the presence of granular hemocytes in the tentacle blastema. (unimore.it)
- Regarding vibrational spectroscopy analysis by FTIR, bands related with biomolecules were shown on mPSCs and DKCs spectra, observing distinct differences between cell lineages and maturation stages. (biomedcentral.com)
- In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)
- The kidney is a complex organ composed of several cell types, and it is conformed approximately by 11,000 nephrons. (biomedcentral.com)
- Thousands of copies of the circular mtDNA are present in most cell types that are packaged by TFAM into higher-order structures called nucleoids1. (regenerativemedicine.net)
- Moreover, the exclusive targeting of tumor stemness cell disregards the potential for other tumor cells to regain stemness and acquire drug resistance. (bvsalud.org)
- In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)
- Within a target cell, RA signaling is activated by the binding of all- trans RA, the biologically active metabolite of vitamin A, to heterodimers of two nuclear receptors, the retinoic acid receptor (RAR) and the retinoid X receptor (RXR). (mdpi.com)