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  • precursors
  • show that early innate lymphoid progenitors (EILPs) are a developmental intermediate between all-lymphoid progenitors (ALPs) and ILC precursors (ILCps) and identify requirements for their generation and further differentiation. (rupress.org)
  • These precursors include cells with robust muscle-forming activity, which when transplanted into animals with injured or defective skeletal musculature, are able to reconstitute both muscle fibers and regenerative satellite cells, improving muscle function and providing enduring muscle regenerative function. (harvard.edu)
  • However, counting CD34+ mononuclear cells may overestimate myeloid blasts in bone marrow smears due to hematogones (B lymphocyte precursors) and CD34+ megakaryocytes. (wikipedia.org)
  • Currently Early Lymphoid Progenitors (ELP) are proposed to settle the thymus and are likely the precursors of at least some thymocytes. (wikipedia.org)
  • G-CSF also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils. (wikipedia.org)
  • human hematopoietic stem
  • Models of acute or chronic leukemia established using cancer cells collected from patients (A complete list of publications is available here) A highly sensitive platform for studying epithelial and cancer stem cells Establishing a functional, humanized immune system from engrafted human hematopoietic stem cells and progenitors Humanized models for studying human-specific infectious diseases like HIV, Epstein Barr virus, malaria, and Dengue fever. (wikipedia.org)
  • cytokine
  • NK cells in the periphery expressing inhibitory receptors that can bind "self" MHC-I are considered licensed with cytokine-producing and cytotoxic functions. (frontiersin.org)
  • Stimulation of CD117 by its ligand, the cytokine stem cell factor (SCF), is important for stem cell survival and maintance. (miltenyibiotec.com)
  • Muse cells are able to generate cells representative of all three germ layers from a single cell both spontaneously and under cytokine induction. (wikipedia.org)
  • CD117 is a cytokine receptor expressed on the surface of hematopoietic stem cells as well as other cell types. (wikipedia.org)
  • citation needed] IL-7 is a cytokine important for B and T cell development. (wikipedia.org)
  • This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. (wikipedia.org)
  • This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRß) during early T cell development. (wikipedia.org)
  • This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. (wikipedia.org)
  • A human study of IL-7 in patients with cancer demonstrated that administration of this cytokine can transiently disrupt the homeostasis of both CD8+ and CD4+ T cells with a commensurate decrease in the percentage of CD4+CD25+Foxp3+ T regulatory cells. (wikipedia.org)
  • citation needed] In order to harvest stem cells from the circulating peripheral blood, blood donors are injected with a cytokine, such as granulocyte-colony stimulating factor (G-CSF), that induces cells to leave the bone marrow and circulate in the blood vessels. (wikipedia.org)
  • maturation
  • Silver and colleagues show that germinal center flexibility permits nonspecific B cells to achieve de novo antigen recognition and antibody affinity maturation. (rupress.org)
  • Enforced expression of the ABCG2 cDNA resulted in a robust SP phenotype and a reduction in hematopoietic maturation. (wiley.com)
  • citation needed] It is important for proliferation during certain stages of B-cell maturation, T and NK cell survival, development and homeostasis. (wikipedia.org)
  • One example is the transformation of iris cells to lens cells in the process of maturation and transformation of retinal pigment epithelium cells into the neural retina during regeneration in adult newt eyes. (wikipedia.org)
  • The fact that transdetermination (change of the path of differentiation) often occurs for a group of cells rather than single cells shows that it is induced rather than part of maturation. (wikipedia.org)
  • Certain cytokines such as IL-3, IL-6, IL-11, LIF, erythropoietin, and thrombopoietin all stimulate the maturation of megakaryocytic progenitor cells. (wikipedia.org)
  • vitro
  • 14 Additional alternatives of lineage branching based on these results have been proposed, 15 , 16 but differences in markers chosen for cell isolation and the use of different in vitro assays to test lineage restriction make these studies difficult to reconcile. (bloodjournal.org)
  • 8 DESIGN AND METHODS Stem Cell Isolation and manipulation Analyses on stem cells before and after manipulation in vitro RESULTS AND DISCUSSIONS. (docplayer.net)
  • They showed that opposing gradients of bone morphogenetic protein (BMP) and Nodal, two transforming growth factor family members that act as morphogens, are sufficient to induce molecular and cellular mechanisms required to organize, in vivo or in vitro, uncommitted cells of the zebrafish blastula animal pole into a well-developed embryo. (wikipedia.org)
  • These properties can be illustrated with relative ease in vitro, using methods such as clonogenic assays, where the progeny of a single cell is characterized. (wikipedia.org)
  • It can be induced by modifying the growth medium when stem cells are cultured in vitro or transplanting them to an organ of the body different from the one they were originally isolated from. (wikipedia.org)
  • Attempts to create organs in vitro started with one of the first dissociation-reaggregation experiment where Henry Van Peters Wilson demonstrated that mechanically dissociated sponge cells can reaggregate and self-organize to generate a whole organism. (wikipedia.org)
  • gene
  • Furthermore, gene therapy and organ transplantation have been performed using bone marrow cells (BMCs) [ 4 - 8 ]. (omicsonline.org)
  • Our ongoing studies are aimed at further defining cell lineage relationships in the differentiation of muscle stem and progenitor cells, enhancing the efficiency with which these cells can be transplanted and regenerate skeletal muscle, as well as identifying signaling pathways and gene expression programs important for maintaining the regenerative potential of these muscle-resident stem cells throughout life and preventing their malignant transformation in the context of myogenic sarcomas. (harvard.edu)
  • Mast/stem cell growth factor receptor (SCFR), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a receptor tyrosine kinase protein that in humans is encoded by the KIT gene. (wikipedia.org)
  • Activating mutations in this gene are associated with gastrointestinal stromal tumors, testicular seminoma, mast cell disease, melanoma, acute myeloid leukemia, while inactivating mutations are associated with the genetic defect piebaldism. (wikipedia.org)
  • The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells. (wikipedia.org)
  • Multiple mutations on GATA2 gene have been recently implicated as the cause of primary immunodeficiency in patients with MonoMAC Syndrome, and cases of dendritic cell, monocyte, B NK lymphoid deficiency and leukemia. (wikipedia.org)
  • Unlike most genes, which have a stable sequence in each cell which expresses them, the T cell receptor is made up of a series of alternative gene fragments. (wikipedia.org)
  • The outcome of this process is that each T cell receptor has a different sequence, due to different choice of gene fragments and the errors introduced during the cutting and joining process (see section on V(D)J recombination for more information on TCR rearrangement). (wikipedia.org)
  • Cluster of differentiation antigen 135 (CD135) also known as fms like tyrosine kinase 3 (FLT-3), receptor-type tyrosine-protein kinase FLT3, or fetal liver kinase-2 (Flk2) is a protein that in humans is encoded by the FLT3 gene. (wikipedia.org)
  • Blood and Marrow Transplantation
  • In recognition of his research accomplishments and leadership in moving basic science discoveries in stem cell biology into the clinic, he was elected President of the International Society of Cellular Therapy (1995-1997), Treasurer of the Foundation for the Accreditation of Cellular Therapy (1995-2002) and President of the American Society of Blood and Marrow Transplantation (1999-2000). (wikipedia.org)
  • regenerative
  • Types of stem cells used for cardiac regenerative therapy. (hindawi.com)
  • To develop equally robust cell therapies for treating non-blood-cell-related disease, cells with equivalent regenerative function for non-blood tissues must be identified. (harvard.edu)
  • Marrow nucleated cells are used every day in regenerative orthopedics. (wikipedia.org)
  • In 2006 Eaves retired as required by provincial law at that time, becoming Professor Emeritus of Hematology and spending more time on several companies he founded to further the fields of cellular therapy and regenerative medicine - STEMCELL Technologies, STEMSOFT Software Inc. and Malachite Management Inc. These companies continue to grow under his leadership and by 2016, STEMCELL had over 900 employees making Canada's largest biotechnology company. (wikipedia.org)
  • Some of the biomedical approaches within the field of regenerative medicine may involve the use of stem cells. (wikipedia.org)
  • reconstitution
  • 11,Recipients of T-cell-depleted bone marrow allografts have a decreased risk of developing graft-vs-host disease,[17,but experience delayed immune reconstitution and have an increased risk of infections, graft failure, and relapse. (cancernetwork.com)
  • Reconstitution of the hematopoietic system during immune responses and immunological and neoplastic diseases or upon transplantation depends on the emergent differentiation of hematopoietic stem/progenitor cells within the bone marrow. (hindawi.com)
  • Total body irradiation (TBI) is part of the preconditioning regimen for allogeneic bone marrow transplantation (alloBMT) and the procedure is associated with treatment-related toxicity and delayed immune reconstitution. (frontiersin.org)
  • We thus undertook a study to assess the effect of TBI on the reconstitution of class I MHC-specific Ly49 NK cell receptors in a rat model of alloBMT. (frontiersin.org)
  • Associated with antiretroviral therapy, IL-7 administration decreased local and systemic inflammations in patients that had incomplete T-cell reconstitution. (wikipedia.org)
  • They suggested that the less mature tissues, or organ buds, generated through the self-organized condensation principle might be the most efficient approach toward the reconstitution of mature organ functions after transplantation, rather than condensates generated from cells of a more advanced stage. (wikipedia.org)
  • fetal
  • Fetal liver kinase-1 (Flk-1) is a cell surface receptor protein that is commonly used as a marker for ESCs and EPCs. (wikipedia.org)
  • cytokines
  • The differentiation and proliferation of CFU-GEMM are promoted by growth factors, such as interleukins and cytokines. (wikipedia.org)
  • The stem cell will follow a specific lineage depending on the presence of certain growth factors and cytokines. (wikipedia.org)
  • The results of studies conducted by Carow, Hangoc, and Broxmeyer in 1993 reveal that the CFU-GEMM can be classified as a stem cell due to its high replating efficiency in the presence of certain growth factors and cytokines. (wikipedia.org)
  • Cells that have the capacity to release soluble factors such as cytokines, chemokines, and growth factors which act in a paracrine or endocrine manner. (wikipedia.org)
  • TGFβ The TGFβ family of cytokines regulate the stemness of both normal and cancer stem cells. (wikipedia.org)
  • Two cytokines that down modulate FLT3 activity (& block FLT3-induced hematopoietic activity) are: TNF-Alpha (Tumor necrosis factor-alpha) TGF-Beta (Transforming growth factor-beta) TGF-Beta especially, decreases FLT3 protein levels and reverses the FLT3L-induced decrease in the time that hematopoietic progenitors spend in the G1-phase of the cell cycle. (wikipedia.org)
  • somatic
  • Hela cells and human fibroblast-derived iPS cells showed high telomerase activity while Muse was at nearly the same level as that in somatic cells such as fibroblasts (these data are shown without running control for the telomerase activity, the comparison is not scientific thought). (wikipedia.org)
  • In contrast, genes related to cell cycle progression and tumorigenicity in Muse cells were at the same level as those in somatic cells, while the same genes were very high in ES and iPS cells. (wikipedia.org)
  • Progenitors are obtained by so-called direct reprogramming or directed differentiation and are also called induced somatic stem cells. (wikipedia.org)
  • Induced totipotent cells can be obtained by reprogramming somatic cells with somatic-cell nuclear transfer (SCNT). (wikipedia.org)
  • The process involves sucking out the nucleus of a somatic (body) cell and injecting it into an oocyte that has had its nucleus removed Using an approach based on the protocol outlined by Tachibana et al. (wikipedia.org)
  • This model has received support from studies showing subpopulations of blood cells harboring initiating but not late somatic mutations in patients with chronic lymphocytic leukemia (CLL), hairy cell leukemia (HCL), and AML. (wikipedia.org)
  • antigen
  • We studied 113 consecutive patients with hematologic malignancies who received non-T-cell-depleted BMT from human leukocyte antigen (HLA)-matched siblings. (cancernetwork.com)
  • survival
  • We characterized the effect of transplanted donor mononuclear cells on relapse- and event-free survival after allogeneic bone marrow transplantation (BMT). (cancernetwork.com)
  • Recipients of larger numbers of DC2 cells had significantly lower event-free survival, a lower incidence of chronic graft-vs-host disease, and an increased incidence of relapse. (cancernetwork.com)
  • 1-A number of factors, including patient disease status, age, conditioning regimen, and (for allogeneic recipients) type of graft-vs-host disease prophylaxis, influence the probability of survival after transplantation. (cancernetwork.com)
  • Nevertheless, all methods aforementioned have been associated with limited cardiac function improvements and limited cell survival once implanted in the fibrous myocardium. (wikipedia.org)