Differentiation of pluripotent embryonic stem cells. Medical search. Frequent questions

and Induced Pluripotent Stem Cells (iPSCs) at the site of injury during stem cell therapy. (nih.gov)
Human embryonic stem cells (ESCs) and human induce pluripotent stem cells (iPSCs) can provide theoretically unlimited supplies of human cardiac myocytes(CMs) for research and potentially clinical applications. (nih.gov)
All cell types being present in a human body may be most probably differentiated from patient-specific iPSCs to provide a cellular panel. (medscape.com)
Moreover, the application of iPSCs would allow personalized testing and therapy during embryonic development. (medscape.com)
2. Integrated analysis of hematopoietic differentiation outcomes and molecular characterization reveals unbiased differentiation capacity and minor transcriptional memory in HPC/HSC-iPSCs. (nih.gov)
The recovery of physiological functionality, which is commonly seen in tissue mimetic three-dimensional (3D) cellular aggregates (organoids, spheroids, acini, etc.), has been observed in cells of many origins (primary tissues, embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and immortal cell lines). (mdpi.com)
We have uncovered several novel transcriptional and post-transcriptional mechanisms that govern the pluripotent state (Figure 3), and are continuing to investigate the function of these regulators in embryonic stem cells, iPSCs, adult stem cells, and mouse development. (nih.gov)
Our goals are to elucidate gene regulatory networks that guide differentiation of photoreceptor subtypes in the mouse and human retina and in retinal organoids derived from embryonic or induced pluripotent stem cells (iPSCs). (nih.gov)
Additionally, they should possess competency in reprogramming PMEFs into induced Pluripotent Stem Cells (iPSCs), and familiarity with the characteristics of teratoma and teratocarcinoma formation and expansion in nude mice. (nih.gov)
Derivation of induced pluripotent stem cells (iPSCs) with transgene-free approaches (i.e., sendai virus or episomal vectors). (ibecbarcelona.eu)
Culture and expansion of human pluripotent stem cells (including human iPSCs and human embryonic stem cells) for further applications in genome editing and differentiation. (ibecbarcelona.eu)
But today, adult, mature cells - such as skin or blood cells - can be "reprogrammed" to generate induced pluripotent stem cells (iPSCs. (bayer.com)
Advanced culturing methods can make it possible to direct the differentiation of the iPSCs to a certain type of cell, like specific brain cells or heart cells. (bayer.com)
These categories include embryonic, peri-natal (obtained from the umbilical cord or from amniotic tissue), somatic adult, or induced pluripotent stem cells (iPSCs). (hindawi.com)
iPSCs are not adult stem cells in origin but mature adult cells that are modified resulting in cell pluripotency and the characteristics of embryonic cells [ 4 , 5 ]. (hindawi.com)
Signs of aging were erased and the iPSCs obtained can produce functional cells, of any type, with an increased proliferation capacity and longevity," explains Jean-Marc Lemaitre who directs the Inserm AVENIR team. (eurekalert.org)
Those reprogramed stem cells are called induced pluripotent stem cells (iPSCs). (nih.gov)
Human pluripotent stem cells (hPSCs), such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), are very attractive cell sources for the treatment of diabetes mellitus, because numerous cells can be obtained using their infinite proliferation potential to overcome the paucity of donor islets. (ehu.eus)
There are reports that suspension culture and spheroid formation promote differentiation in various cell types, including hPSCs, but, to our knowledge, there are no reports comparing gene expression patterns between suspension and adherent cultured human iPSCs (hiPSCs) during definitive endoderm (DE) differentiation. (ehu.eus)
The Guo Lab applies cutting-edge stem cell technologies including iPSCs, organoids and transdifferentiation to study the etiology of neurodevelopmental (e.g. schizophrenia and autism) and neurodegenerative (e.g. (cincinnatichildrens.org)
Various pluripotent stem cells (PSCs), such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), are used in various biomedical fields due to their capacity of unlimited self-renewal and the ability to differentiate into multiple cell types [ 12 ]. (springer.com)

They must have experience working with mouse Embryonic Stem Cells (mESCs) and Primary Mouse Embryonic Fibroblasts (PMEFs), along with demonstrated skill in in vitro differentiation of mESCs. (nih.gov)
In the present study, in addition to conventional viability assessments, we evaluated the gene expression using RNA-seq to investigate whether the developed bioprinter influenced cell integrity in mouse embryonic stem cells. (nature.com)
Transgenic reporter mouse embryonic stem cells were used in this study. (siu.edu)
To promote adhesion of mouse embryonic stem cells and to initiate integrin-based mechanical signaling, the extracellular matrix protein fibronectin was used. (siu.edu)
In the presence of fibronectin and a small molecule called leukemia inhibitory factor (LIF), which is known to maintain pluripotency and self-renewal, mouse embryonic stem cells responded by exhibiting expressions of both GFP and DsRed. (siu.edu)
Increased apoptosis in differentiating p27-deficient mouse embryonic stem cells. (muni.cz)
The reverse tetracycline-controlled transactivator rtTA2s-S2 is toxic in mouse embryonic stem cells. (muni.cz)

The primary focus of the FOA is to promote in vivo studies of stem cells in animal models and in humans (if applicable) to better understand how stem cells function within developing or damaged tissues. (nih.gov)
Furthermore, it is expected that replicating developmental mechanisms would ameliorate the safety concerns associated with incomplete differentiation and improper integration of cells in damaged or diseased tissues during stem cell therapy. (nih.gov)
After 25 minutes in a 37 degree Celsius water bath, stop the reaction with one milliliter of PBS plus FBS and use a one milliliter pipette tip to triturate the tissues 20 to 30 times until a single cell suspension is obtained. (jove.com)
If these mechanisms can be harnessed to obtain cells specifically required for therapy, diagnosis or drug discovery, it may be possible to restore function to tissues and organ systems that have been compromised by congenital disorders, developmental malfunction, age, injury, disease or drug exposure. (nih.gov)
Although animal studies demonstrate that stem or progenitor cells can be derived from a variety of tissues and from hosts of different ages, the requirements and potential for differentiation of each type of pluripotent cell appear to be unique. (nih.gov)
Biomaterials that control stem cell function - While natural tissues are three dimensional structures with complex biochemical and biophysical properties, cell culture systems typically involve a two dimensional, rock hard surface with liquid on top. (berkeley.edu)
Few subjects in biomedical science have captured the imagination of both the scientific community and the public as has the use of stem cells for the repair of damaged tissues. (jci.org)
The use of telomerase to promote regeneration of human tissues,2 and the new discovery that adult stem cells may be far more versatile than was once thought,3 offer the promise that embryonic stem cells may simply be irrelevant to future medical progress. (usccb.org)
This discovery, from a study led by researchers from Brigham and Women's Hospital and Harvard Medical School (HMS), suggests that faulty embryonic stem cells have a built-in mechanism to ensure that they are destroyed before they can compromise the functioning of future cells and tissues. (genengnews.com)
Instead of looking at genes one by one, we looked at thousands of genetic alterations at the same time to determine how they affect the proliferation of embryonic stem cells, and, subsequently, the development of the three germ layers that serve as the raw material for human tissues. (genengnews.com)
This research paves the way for the therapeutic use of iPS, insofar as an ideal source of adult cells is provided, which are tolerated by the immune system and can repair organs or tissues in elderly patients. (eurekalert.org)
Due to the heterogeneity of tissues and cell types involved in early pregnancy establishment (i.e., gametes, embryo, uterus, immune cells, endocrine cells, endothelial cells, stromal cells, etc.), use of 'omics approaches at the single-cell level is particularly encouraged. (nih.gov)
To name a few, tissues derived from the neural crest include pigment cells in the skin, the entire peripheral nervous system, the bones of the face and neck, parts of the heart, and several hormone-producing cell types - in practice, cell types whose differentiation from a single germ layer should be impossible. (nih.gov)
The inner cell mass will ultimately develop into the specialized cell types, tissues, and organs of the entire body of the organism. (nih.gov)
Adult stem cells have been identified in many organs and tissues and are generally associated with specific anatomical locations. (nih.gov)
These stem cells may remain quiescent (non-dividing) for long periods of time until they are activated by a normal need for more cells to maintain and repair tissues. (nih.gov)
Stem cells have unique abilities to self-renew and to recreate functional tissues. (nih.gov)
Stem cells have the ability to recreate functional tissues. (nih.gov)
Leveraging information from organogenesis in animal models we are establishing new methods to direct the differentiation PSCs into 3-dimensional organ tissues called organoids. (cincinnatichildrens.org)

To elucidate cellular events underlying the pluripotency of human embryonic stem cells (hESCs), we performed parallel quantitative proteomic and phosphoproteomic analyses of hESCs during differentiation initiated by a diacylglycerol analog or transfer to media that had not been conditioned by feeder cells. (nih.gov)
These data are a resource for studies of the events associated with the maintenance of hESC pluripotency and those accompanying their differentiation. (nih.gov)
We found a specific interaction of DNMTs during early differentiation with the PAF1 (polymerase-associated factor 1) transcriptional elongation complex, which binds to promoters of the pluripotency and known DNMT target genes encoding OCT4 and NANOG, thereby providing a possible molecular link for the silencing of these genes during differentiation. (nih.gov)
The cells show normal diploid karyotype and continue to express all pluripotency markers. (amrita.edu)
To fully realize these potentials, however, it is important to understand the molecular basis of the defining characteristic of the pluripotent cells: pluripotency. (nih.gov)
They maintain pluripotency, and they keep apoptosis, or programmed cell death, on a hair trigger. (genengnews.com)
Joint analysis of all screens revealed that genetic alterations that broadly inhibit differentiation across multiple germ layers drive proliferation and survival under pluripotency-maintaining conditions and coincide with known cancer drivers. (genengnews.com)
Pluripotency means that the cells can differentiate into any cell type of the human body. (bayer.com)
Embryonic stem cell (ESC) culture conditions are important for maintaining long-term self-renewal, and they influence cellular pluripotency state. (nih.gov)
However, this masks variable expression of pluripotency genes in serum cells and homogeneous expression in 2i and a2i cells. (nih.gov)
The reporter cell line shows the cellular endogenous activity of pluripotency gene Oct3/4 with green fluorescent protein (GFP) while also reporting mesoderm-specific gene, Brachyury, activity with DsRed fluorescent protein. (siu.edu)
The potential of stem cell therapies is based on this pluripotency of early embryonic stem cells. (nih.gov)
Gastrulation is a crucial stage in embryonic development where this pluripotency is lost when the germ layers of the embryo (ectoderm, mesoderm and endoderm) and the three body axes of the developing body (anterior and posterior, dorsal and ventral, left and right) are formed. (nih.gov)

Disease model systems can be obtained from reprogrammed disease-specific somatic cells, normal PSCs genetically modified to mimic genetic variations related to a specific disease or ESCs derived from an embryo with a known genetic mutation (Figure 2). (medscape.com)
The common stress responses of the blastocyst-derived stem cells, pluripotent embryonic and multipotent placental trophoblast stem cells (ESCs and TSCs), are decreased growth and potency, and increased, imbalanced and irreversible differentiation. (nih.gov)
The outcome of p53 activation is to induce the differentiation of ESCs into other cell types that they can go into a senescent state or induces apoptosis to preserve genome stability. (aging-us.com)
This study investigated the molecular and epigenetic mechanisms that lead to the formation of muscle and neuronal cells from embryonic stem cells (ESCs) at the single-cell level. (nih.gov)
This study identified the underlying mechanisms that regulate the differentiation of ESCs into muscle and neuronal cells. (nih.gov)
This paper provides valuable data for understanding how muscle cells are specified and committed, and for generating muscle progenitors starting from ESCs or induced pluripotent stem cells. (nih.gov)
Transcriptomics, regulatory syntax, and enhancer identification in mesoderm-induced ESCs at single-cell resolution. (nih.gov)

The latter are of great interest as diverse cytokines have been shown to regulate the differentiation of mature blood cells from adult HSC. (haematologica.org)
In this regard, a major effort is being made to generate mature, adult phenotypes of the differentiated progenies and to harvest pure and homogenous populations of the desired cell types on a large scale. (medscape.com)
7. In vitro conversion of adult murine endothelial cells to hematopoietic stem cells. (nih.gov)
Pluripotent cells have the ability to develop into all cells of the adult body, and therefore show great promises in both basic and translational research. (nih.gov)
Stem cells, defined by their abilities to self-renew (divide in an immature state) and differentiate into specialized cells, play critical roles in organismal development and adult tissue function. (berkeley.edu)
However, advances in this field will hinge upon gaining a deeper basic understanding of how stem cell function is regulated, and our group works with adult neural stem cells and human pluripotent stem cells to investigate this problem. (berkeley.edu)
Adult neurogenesis, the generation of new neurons from stem cells in the adult brain, modulates learning and memory and can decrease during neurodegeneration. (berkeley.edu)
a) Neural stem cells undergoing proliferation in the hippocampus (BrdU, blue) of the adult brain can later undergo differentiation into granule neurons (NeuN, green) or astrocytes (GFAP, red). (berkeley.edu)
For example, neural stem cells within the adult hippocampus are influenced by a repertoire of molecules representative of each of these classes, several of which our lab has discovered. (berkeley.edu)
Human embryonic stem cells offer the promise of a new regenerative medicine in which damaged adult cells can be replaced with new cells. (jci.org)
At the December 2 hearing, responding to our proposed list of promising alternatives to embryonic stem cell research, National Institutes of Health director Harold Varmus said that while adult stem cells can be obtained from bone marrow, cord blood and so on, they are of limited use because they cannot form other kinds of tissue such as nerve and skin. (usccb.org)
We will develop advanced 3D skeletal muscle-motoneuron microtissues from human pluripotent stem cells that accurately mimic human muscle development from embryonic to adult stages. (mskcc.org)
Dental stem cells are adult stem cells, one of the two major divisions of stem cell research. (sciencedaily.com)
Adult stem cells have the ability to regenerate many different types of cells, promising great therapeutic potential, especially for diseases such as Huntington's and Parkinson's. (sciencedaily.com)
By showing dental pulp stem cells are capable of stimulating growth of neurons, our study demonstrates the specific therapeutic potential of dental pulp stem cells and the broader potential for adult stem cells," says Chan, who also is assistant professor of human genetics in Emory School of Medicine. (sciencedaily.com)
However, the differentiation process of hPSCs is much more complex than adult stem cells, and the unknown effect of material addition at different stages hamper the establish of step-wise induction systems for these human pluripotent stem cells (hPSCs). (researchsquare.com)
While there is some overlap, the most common categories of adult stem cells are mesenchymal stem cells (MSCs) and hematopoietic stem cells which are both defined based upon specific stem cell surface markers [ 3 ]. (hindawi.com)
Adult stem cell research also holds much promise, as is endlessly pointed out by embryonic stem cell foes. (wheelchairkamikaze.com)
Many of the much heralded advances in adult stem cell research involve perfecting ways to get adult stem cells to behave like embryonic stem cells. (wheelchairkamikaze.com)
For now, I am in favor of adult stem cell research and hope the answer to incurable diseases will be found here. (wheelchairkamikaze.com)
They can divide and form all types of differentiated adult cells in the body (neurones, cardiac cells, skin cells, liver cells, etc. (eurekalert.org)
Since 2007, a handful of research teams across the world have been capable of reprogramming human adult cells into induced pluripotent cells (iPSC), which have similar characteristics and potential to human embryonic stem cells (hESC). (eurekalert.org)
The rejuvenated iPSC cells were again differentiated to adult cells and compared to the original old cells, as well as to those obtained using human embryonic pluripotetent stem cells (hESC). (eurekalert.org)
There are several main categories: the "pluripotent" stem cells (embryonic stem cells and induced pluripotent stem cells) and nonembryonic or somatic stem cells (commonly called "adult" stem cells). (nih.gov)
Pluripotent stem cells have the ability to differentiate into all of the cells of the adult body. (nih.gov)
Adult stem cells are found in a tissue or organ and can differentiate to yield the specialized cell types of that tissue or organ. (nih.gov)
In 2006, researchers identified conditions that would allow some mature human adult cells to be reprogrammed into an embryonic stem cell-like state. (nih.gov)
Throughout the life of the organism, populations of adult stem cells serve as an internal repair system that generates replacements for cells that are lost through normal wear and tear, injury, or disease. (nih.gov)
On the other hand, adult stem cells differentiate to yield the specialized cell types of the tissue or organ in which they reside, and may have defining morphological features and patterns of gene expression reflective of that tissue. (nih.gov)
The adult corneal epithelium is continuously regenerated from stem cells (SC) located at the basal layer of the limbal epithelium. (molvis.org)
We discuss the characteristics and limitations of various types of human embryonic and adult stem cells, and their utility for bone tissue engineering. (biomedcentral.com)

Retinal organoids generated from human pluripotent stem cells exhibit considerable variability during differentiation. (nih.gov)

We identified KDR+/CD34high/CD144+/CD43-/CD45- hemato-endothelial progenitor cells (HEPs) forming organized, vasculature-like structures and giving rise to CD34low/CD144-/CD43+/CD45+ hematopoietic progenitor cells. (haematologica.org)
Thus, our data suggest an important role for IL-3 in early human hematopoiesis by supporting the endothelial to hematopoietic transition of hemato-endothelial progenitor cells and highlight the potential of a hemanoid-based model to study human hematopoietic development. (haematologica.org)
Different cell-intrinsic and extrinsic factors direct the specification of early mesodermal progenitor cells towards hematopoietic stem cells (HSC), which arise in the aorta-gonad-mesonephros (AGM) region, mature in the fetal liver and finally home and reside in the bone marrow. (haematologica.org)
1. Derivation of Hematopoietic Stem and Progenitor Cells from Human Pluripotent Stem Cells. (nih.gov)
19. Respecifying human iPSC-derived blood cells into highly engraftable hematopoietic stem and progenitor cells with a single factor. (nih.gov)
In animal studies, multipotent progenitor cells from many different sources have been reported to generate cells with neuronal or glial properties, raising expectations that they could be used to replace lost neurons and glia, repair defective circuits, and restore functions compromised by abnormal development, age, physical damage, disease or drug addiction. (nih.gov)
We first generated retinal progenitor cells (RPCs) from hESCs then promoted the Notch signaling pathway using Notch ligands, including Delta-like ligand 4 and Jagged-1. (frontiersin.org)
Using AMSBIO's revolutionary Skeletal Muscle Differentiation kit allows researchers to generate muscle from human pluripotent stem cells in 3 easy steps, via satellite-like or progenitor cells and myoblasts that then fuse to multinucleated myotubes in the third step. (onenucleus.com)
The higher numbers of BM-residing VSELs in these animals also correlated with higher numbers of hematopoietic stem progenitor cells (HSPCs) in BM. (biomedcentral.com)

We propose to sponsor a CIRM Research Training Program in stem cell and regenerative medicine, which will provide six postdoctoral Ph.D. or M.D. (Type III) trainees per year with state-of-the-art stem cell-related research experience and coursework in a rich scientific environment. (ca.gov)
The goal is to prepare trainees for productive, independent research careers in stem cell and regenerative medicine, with an emphasis on training individuals from scientifically and demographically diverse backgrounds. (ca.gov)
Trainees will also be invited to participate in other courses and related activities at the Institute, including weekly laboratory meetings, a weekly journal club in stem cell and regenerative medicine, a special lecture series titled Seminars in Stem Cell Biology, professional development workshops, and a Summer Scholars Program for high school and college students, whom trainees will participate in mentoring. (ca.gov)
Findings suggest dental pulp stem cells show promise for use in cell therapy and regenerative medicine, particularly therapies associated with the central nervous system. (sciencedaily.com)
Oct. 10, 2023 Generating specific cell lineages from induced pluripotent stem cells and embryonic stem cells is the holy grail of regenerative medicine. (sciencedaily.com)
The latter exhibit better abilities in both long-term self-renewal and specialized differentiation, becoming an outstanding cell source in the field of regenerative medicine 3 , 4 . (researchsquare.com)
Regenerative medicine and cell therapy become more and more favorite [ 1 ] day by day to overcome the adverse effects of drug. (omicsonline.org)
With so many deaths around the world being due to cardiovascular disease, there is great demand for an unlimited supply of cells for application in regenerative medicine. (siu.edu)
The results represent significant progress for research into iPSC cells and a further step forwards for regenerative medicine. (eurekalert.org)
These findings have the potential to provide important insights into muscle regenerative medicine through "in-a-dish" modeling of muscle disease, drug screening, and cell therapeutics. (nih.gov)
Deciphering the fundamental mechanisms controlling cardiac specification is critical for our understanding of how heart formation is initiated during embryonic development and for applying stem cell biology to regenerative medicine and disease modeling. (xenbase.org)

Comparative neurotoxicity screening in human iPSC-derived neural stem cells, neurons and astrocytes. (nih.gov)
Of high priority are studies to develop methods for identifying, isolating and characterizing specific precursor populations at intermediate stages of differentiation into neurons and glia, and their relationship to tumor- generating cells. (nih.gov)
c) They can also undergo differentiation into astrocytes (GFAP, red) and (at least in vitro) oligodendrocytes (O4, green) upon addition of FBS, or d) into neurons (NF200, green) upon addition of forskolin and retinoic acid. (berkeley.edu)
Research is needed to determine the most viable stem cell lines and reliable ways to promote the differentiation of pluripotent stem cells into specific cell types (neurons, muscle cells, etc. (jci.org)
Eventually a stem cell becomes known as a "progenitor" or "precursor" cell, committed to producing one or a few terminally differentiated cells such as neurons or muscle cells. (jci.org)
The dental pulp stem cells stimulated growth of new neural cells, and many of these formed neurons. (sciencedaily.com)
During his Ph.D. studies, he identified a novel genetic pathway controlling neurogenesis during the development of neural stem cells to midbrain dopaminergic neurons. (nih.gov)
These complex multi-lineage organoids contain a diversity of epithelial and mesencymal cell types similar to the normal GI tract and can be reconstituted with enteric neurons. (cincinnatichildrens.org)

They looked at eight types of cells in various stages of commitment, including very early blood stem cells that had yet to differentiate into red and white blood cells. (hopkinsmedicine.org)
That's why we wanted to know what was happening generally to the levels of DNA methylation as cells differentiate. (hopkinsmedicine.org)
One of the surprising finds was how widely DNA methylation patterns vary in cells as they differentiate. (hopkinsmedicine.org)
Both cell lines differentiate to derivatives of all three germ layers in vitro. (amrita.edu)
Despite their important contribution to retinal homeostasis, a specific way to differentiate retinal glial cells from human pluripotent stem cells has not yet been described. (frontiersin.org)
Here, we report a method to differentiate retinal glial cells from human embryonic stem cells (hESCs) through promoting the Notch signaling pathway. (frontiersin.org)
Some of the partially differentiated cells may de-differentiate to become pluripotent cells. (nih.gov)
Notably, during the in vivo embryonic development, mesoderm and ectoderm cells differentiate toward mesenchymal cells, which further differentiate into osteogenic precursor cells and osteoblasts 10 , 11 . (researchsquare.com)
AMSBIO offers a Skeletal Muscle Differentiation kit that enables you to differentiate human pluripotent stem cells to skeletal muscle myotubes with high yields, without cell sorting or genetic manipulation. (onenucleus.com)
MSCs are defined by three characteristics: (i) an ability to adhere to plastic, (ii) presentation of stem cell specific antigens, and (iii) the potential to form multipotent mesenchymal cells which can differentiate into a number of cell lines interesting to musculoskeletal medicine such as osteocytes, chondrocytes, and adipocytes [ 7 - 9 ]. (hindawi.com)
Appropriate induction medium was used to differentiate these cells into adipocytes, osteocytes, and chondrocytes. (molvis.org)
Early passaged cells when induced were able to differentiate into adipocytes, osteocytes and chondrocytes. (molvis.org)
In intramembanous ossification, cells of the condensed mesenchymal tissue differentiate into osteoblasts and directly form bone. (biomedcentral.com)

ferentiation potential of normal Bombay hiPSCs (B-hiPSCs) and compared results to human embryonic stem cell (hESC) lines. (1library.org)
Many in the international scientific community believe that the promise of stem cell-based studies or therapies will be realized only if we can derive new human embryonic stem cell (hESC) lines. (jci.org)
These old cells were reprogrammed in vitro to induced pluripotent stem cells (iPSC) and to rejuvenated and human embryonic stem cells (hESC): cells of all types can again be differentiated after this genuine "rejuvenation" therapy. (eurekalert.org)
Human embryonic stem cells (hESC) are undifferentiated multiple-function cells. (eurekalert.org)

The Stem Cell Biology Group aims to understand the regulation of the pluripotent state in cells in culture and during early embryonic development. (nih.gov)
A new discovery by researchers challenges our current understanding of gastrulation, the most important stage of early embryonic development. (nih.gov)
Our findings shed new light on the chain of events in early embryonic development. (nih.gov)

7 Among others, interleukin-3 (IL-3), formerly known as multi colony stimulating factor, has been implicated in survival, proliferation, and differentiation of HSC and hematopoietic progenitor populations. (haematologica.org)
Using signaling molecules conjugated to polymers in controlled fashions, akin to beads on a string, we have shown that nanoscale organization in the presentation of numerous ligands (e.g. ephrins, Shh, FGF) results in 10-100 fold higher potency in inducing the proliferation or differentiation of neural stem cells and human embryonic and induced pluripotent stem cells. (berkeley.edu)
The researchers used genome-wide genetic screening to both overexpress and inactivate ("knock out") tens of thousands of genes that govern embryonic stem cell proliferation and differentiation into the three germ layers. (genengnews.com)
Researchers first multiplied skin cells (fibroblasts) from a 74 year-old donor to obtain the senescence characterized by the end of cellular proliferation. (eurekalert.org)
Aryl Hydrocarbon Receptor-Activating Polychlorinated Biphenyls and Their Hydroxylated Metabolites Induce Cell Proliferation in Contact-Inhibited Rat Liver Epithelial Cells. (muni.cz)

This change is found in one of the building blocks of DNA, is remembered by a cell when it divides, and often is associated with turning off genes. (hopkinsmedicine.org)
Genes themselves aren't going to tell us what's really responsible for the great diversity in cell types in a complex organism like ourselves," Feinberg says. (hopkinsmedicine.org)
The data shows that when all is said and done, the lymphocytes had many more methylated genes than myeloid cells. (hopkinsmedicine.org)
However, the extension of these successes to most human diseases is impeded by a number of shortcomings of the viral vehicles used to carry therapeutic genes into cells, which are not surprising since nature never "intended" viruses to be used for human therapy. (berkeley.edu)
Details of the work appeared October 28 in the journal Genes and Development , in an article titled, " Integrated loss- and gain-of-function screens define a core network governing human embryonic stem cell behavior . (genengnews.com)
Finally, the researchers observed that many of the genes that regulate the formation of the three germ layers also are known contributors to the growth of cancers when they are over- or underexpressed in somatic cells. (genengnews.com)
Moreover, the expression of marker genes relating to germ layers and epithelial-mesenchymal transition (EMT) was investigated at day 7, aiming to study the impact of BFP1 peptide on the lineage differentiation and osteogenic induction process of hESCs and hiPSCs. (researchsquare.com)
Additionally, genes related to the cell cycle are more variably expressed in the 2i and a2i conditions. (nih.gov)
The findings indicate that the expression stage of stem cell genes needed for the production of pluripotent stem cells continues throughout the ectoderm almost until the completion of the neural tube, a precursor to the central nervous system. (nih.gov)
Previously, the research group developed this technique for the simultaneous analysis of the expression of several genes, which they modified further for this study to enable them to monitor the location of individual stem cells in the ectoderm at different stages of development following gastrulation. (nih.gov)
The report identifies regions of the genome called enhancers that control genes critical for muscle and neuronal cell differentiation. (nih.gov)
SSEA, Oct-4, Nanog), are a population of early-development stem cells that, due to epigenetic changes in certain paternally imprinted genes (e.g. (biomedcentral.com)
However, VSELS have the potential to become specified into more-differentiated tissue-committed stem cells (TCSCs) after reversing expression of imprinted genes to the somatic type [ 5 ]. (biomedcentral.com)
Our results demonstrate that expressions of these marker genes are faster and more strongly induced in suspension culture than in adherent culture during the DE differentiation process, indicating that suspension culture favors DE differentiation. (ehu.eus)

This work has shed light on the mechanisms behind the neurogenic-to-gliogenic switch in human neural stem cells and enables the rapid generation of astrocytes for the use in human disease modeling. (cincinnatichildrens.org)

Using this new "cocktail" of six factors, the senescent cells, programmed into functional iPSC cells, re-acquired the characteristics of embryonic pluripotent stem cells. (eurekalert.org)

some variations in the eficiency of hematopoietic differentiation between iPSC and ES cells, the pattern of differentiation was similar among all three tested lines. (1library.org)
Here, we used induced pluripotent stem cell (iPSC) technology and established a 3-dimensional, organoid-like differentiation system (hemanoid) maintaining the structural cellular integrity to evaluate the effect of cytokines on embryonic hematopoietic development. (haematologica.org)
Germano established a basic science laboratory within the neurosurgery department to focus on brain tumors translational research with particular emphasis on gene therapy, stem cells, and induced pluripotent stem cells (iPSC). (wikipedia.org)
One apparent application of this work might be to employ these same techniques to assess how completely an induced pluripotent stem cell (iPSC) has been reprogrammed. (hopkinsmedicine.org)
The researchers proved that the iPSC cells thus obtained then had the capacity to reform all types of human cells. (eurekalert.org)
The Gu Lab utilizes patient-specific iPSC derived endothelial and smooth muscle cells, as well as vessel organoids to explore the role of vascular deficiency in the etiology of heart, lung, and brain diseases. (cincinnatichildrens.org)

The derivation and maintenance of human pluripotent stem cells (hPSCs) in stable naïve pluripotent states has a wide impact in human developmental biology. (nih.gov)
Guang Hu, Ph.D., leads the Stem Cell Biology Group within the Epigenetics and Stem Cell Biology Laboratory, and holds a secondary appointment in the NIEHS Reproductive and Developmental Biology Laboratory . (nih.gov)
We use a combination of functional genetics, genomics, cell biology, and developmental biology tools to systematically identify and dissect the function of regulators in pluripotent cells (Figure 2). (nih.gov)
Our research program melds biology and engineering principles to investigate stem cells and therapeutic gene delivery, i.e. cell replacement and gene replacement approaches to treat human disease. (berkeley.edu)
To engage in this debate, it is important to have an overview of stem cell biology. (jci.org)
In its effort to find that federal funding of embryonic stem cell research is consistent with congressional intent, HHS has overlooked some obvious facts, and created its own arbitrary definition of a human embryo that has no basis in biology or federal law. (usccb.org)
The aims proposed will provide valuable human stem cell methodologies to an emerging area of muscle biology, new insights to actin regulation during muscle differentiation, and new avenues to understand and potentially treat a muscle myopathy for which there are no therapies. (mskcc.org)
During the current reporting period, the scientific training program has provided support for postdoctoral fellows to receive instruction and engage in research related to stem cell biology. (ca.gov)
Genetic screens present a wonderful opportunity to probe how genetic networks contribute to interrelated cellular behaviors like growth, differentiation, and survival," added Naxerova, who is now an assistant professor in the Center for Systems Biology at Massachusetts General Hospital. (genengnews.com)
Jason D Doles, Ph.D, an assistant Professor of Biochemistry and Molecular Biology and head of the Doles Lab in Rochester, Minnesota commented "The myotube differentiation kit from AMSBIO is simple to use and it has produced consistent results every time. (onenucleus.com)
Dr Doles and the research team in his Skeletal Muscle Wasting and Progenitor Cell Biology group are currently studying muscle regeneration, wasting disorders and stem cell activation using diverse experimental model systems and cutting-edge cell and molecular biology tools and technologies. (onenucleus.com)
The Hofmann Laboratory studies the biology of male germ line stem cells, which are precursors of sperm cells. (mdanderson.org)
Other research in the lab is focused on the pathogenesis and biology of thyroid cancer cells and cancer stem cells. (mdanderson.org)
Before joining NCATS in May 2018, Jovanovic obtained his doctorate in physiology and cell biology from Ben-Gurion University of the Negev in Israel. (nih.gov)

We had earlier reported the derivation and characterization of two new sibling human embryonic stem cell lines BJNhem19 and BJNhem20, from discarded grade III embryos of Indian origin. (amrita.edu)
In this regard, many kinds of growth factors have been applied to establish robust and efficient induction methods for the derivation of osteoblast like cells from hPSCs 8 , 9 . (researchsquare.com)
Derivation and characterization of new human embryonic stem cell lines in Czech Republic. (muni.cz)

Characterization of fenofibrate-mediated anti-proliferative pro-apoptotic effects on high-grade glioma and anti-invasive effects on glioma stem cells. (wikipedia.org)
15. Generation and characterization of integration-free induced pluripotent stem cells from patients with autoimmune disease. (nih.gov)
CHARACTERIZATION, BEHAVIOR AND PLASTICITY OF PLURIPOTENT STEM CELLS RELEASE DATE: May 4, 2004 PA NUMBER: PA-04-101 The R01 portion of this funding opportunity has been replaced by PA-07-201 , which now uses the electronic SF424 (R&R) application for February 5, 2007 submission dates and beyond. (nih.gov)

ac-companied by endothelial cells differentiation. (1library.org)
3-5 The emergence of hematopoietic cells from the HE (also referred to as endothelial to hematopoietic transition [EHT]), is controlled by distinct cell-intrinsic signals mediated by transcription factor (TF) activation, as well as important cell-extrinsic factors such as cell-cell interactions, extracellular matrix molecules, and cytokines. (haematologica.org)
16. Preferential Hematopoietic Differentiation in Induced Pluripotent Stem Cells Derived From Human Umbilical Cord Arterial Endothelial Cells. (nih.gov)
We combine index sorting of single cells from the embryonic aorta-gonad-mesonephros region with endothelial cell co-culture and transplantation to characterize the phenotypic properties and engraftment potential of single hematopoietic precursors. (jove.com)
Negative stem cell markers indicate other cell lineages such as hematopoietic and endothelial cells and include CD 14, 31, 34, and 45 [ 14 - 17 ]. (hindawi.com)
It consists primarily of three layers: an outer layer of epithelium, a middle stromal layer of collagen-rich extracellular matrix (ECM) interspersed with keratocytes and an inner layer of endothelial cells. (molvis.org)
The Takebe lab has pioneered the development of complex liver organoids made by combining hPSC-derived hepatoblasts, endothelial cells and mesenchyme. (cincinnatichildrens.org)

4 Retinal Degeneration Lab and National Stem Cell Bank - Valencia Node, Research Center Principe Felipe, Valencia, Spain. (nih.gov)
The studies in the Swaroop laboratory have focused primarily on 1) genetic and epigenetic regulation of photoreceptor development and aging, 2) genetic defects and mechanisms of photoreceptor dysfunction in retinal neurodegeneration, 3) genetics of age-related macular degeneration, and 4) design of new therapeutic paradigms using cell, gene or small molecule-based approaches. (nih.gov)
Significant progress has been made in cell replacement therapy for neural retinal diseases using retinal cells differentiated from human pluripotent stem cells. (biomedcentral.com)
Here, we attempted to improve the yield of photoreceptor precursor cells in three-dimensional retinal organoids from human embryonic stem cells (hESCs). (biomedcentral.com)
CRX-positive cells can be spatiotemporally tracked by tdTomato without affecting retinalization during retinal organoid differentiation. (biomedcentral.com)
Tracing with the CRX-reporter system showed that in retinal organoids derived from human pluripotent stem cells, COCO increased the differentiation efficiency of photoreceptor precursors and cones. (biomedcentral.com)
The majority of retinal cell types can be formed by in vitro differentiation of pluripotent stem cells (PSCs). (biomedcentral.com)
Compared with the single cell type obtained from most 2D differentiation, the multiple types of retinal cells obtained in a 3D differentiation system form well-laminated and properly stratified structures similar to the real retina, which are obviously closer to the physiological state [ 10 , 11 ]. (biomedcentral.com)
However, increasing the ratio of a certain type of cell is challenging in 3D culturing because of existing inherent cellular programs that form retinal organoids with self-organization. (biomedcentral.com)
Dysfunction of retinal glial cells, particularly Müller cells, has been implicated in several retinal diseases. (frontiersin.org)
This retinal glial differentiation method will help advance the generation of stem cell disease models to study the pathogenesis of retinal diseases associated with glial dysfunction such as macular telangiectasia type 2. (frontiersin.org)
This method may also be useful for the development of future therapeutics such as drug screening and gene editing using patient-derived retinal glial cells. (frontiersin.org)
Retinal glial cells consist of astrocytes, Müller cells and microglia. (frontiersin.org)
To date, there is no report of a differentiation method in adherent culture that specifically produces retinal glial cells. (frontiersin.org)

Genetic lineage tracing confirmed that these mesenchymal cells arose, in part, through a process of epithelial-to-mesenchymal transitioning (EMT). (diabetesjournals.org)

The application of PSCs and their derivatives offer new possibilities to recognize as yet unknown drug targets, reduce the need for experimental animal models and allow high-throughput human cell-based screenings. (medscape.com)
Disease-specific PSCs can be expanded almost infinitely and differentiated into cells with the disease-affected tissue phenotype to assure reproducible large quantities of relevant cell types for pharmacological and toxicological screenings. (medscape.com)
PSCs are excellent model systems to screen for cytokines, chemokines, growth factors and morphogenes that govern tissue development and cell differentiation. (medscape.com)
When it comes to the development of innovative cell therapy treatments, the development of pluripotent stem cells (PSCs) constitutes a quantum leap. (bayer.com)
Human pluripotent stem cells (PSCs) can be grown indefinitely in a petri dish and have the potential to form any cell or tissue type in the body. (cincinnatichildrens.org)

Fluorescent labeling of target cells during in vitro differentiation has played a huge role in organoid research [ 14 ]. (biomedcentral.com)
However, the current in vitro differentiation protocols are inefficient, resulting in a mixture of undifferentiated, partially differentiated and fully differentiated cells. (nih.gov)

Fluorescence intensity of organoids, which turned out highly consistent with flow cytometry measurement, allowed us to determine the differentiation efficiency of precursors during organoid culturing directly. (biomedcentral.com)
Nonetheless, several successful cases have been reported by artificially transforming the cell tendency in the natural state of organoids through external signal pathway intervention. (biomedcentral.com)
However, these organoids consist of heterogenous cell types in suspension culture, which limits the downstream analysis assays that can be performed. (frontiersin.org)
We apply the knowledge learned from animal models to direct the differentiation of human pluripotent stem cells (hPSCs)into organoids. (cincinnatichildrens.org)
With CuSTOM, the Tchieu Lab's focus is on using stem cell-derived glial cells and innovative brain organoids to study early human neurodevelopment and apply this knowledge in understanding how neurological diseases manifest. (cincinnatichildrens.org)

This approach may lead to deciphering the underlying mechanisms of certain diseases and permit the development of new drugs targeting cellular differentiation and morphogenesis at the embryonic stage. (medscape.com)
Current models of bone formation in vitro are based on the paradigm that cellular differentiation and function can be modulated by the same factors known to play a role during embryonic development [ 8 ]. (biomedcentral.com)

The presence of multipotent human limbal stromal cells resembling mesenchymal stromal cells (MSC) provides new insights to the characteristic of these cells and its therapeutic potential. (molvis.org)
The phenotypes and multipotent differentiation potential of these cells were also evaluated. (molvis.org)
The expanded limbal stromal cells showed features of multipotent MSC. (molvis.org)

The in vitro development of blastocyst-derived embryonic stem cell lines:formation of visceral yolk sac, blood islands and myocardium. (nih.gov)
Blastocyst-Derived Stem Cell Populations under Stress: Impact of Nutrition and Metabolism on Stem Cell Potency Loss and Miscarriage. (nih.gov)

Treatment-specific diagnostic tests developed especially for compatibility screenings may require large quantities of easily accessible patient-specific cells with diverse tissue phenotypes. (medscape.com)
In PSC-based applications not only the disease-affected tissue-specific cells but also the early stages of the disease-specific embryonic development can be studied. (medscape.com)
Having charted the occurrence of a common chemical change that takes place while stem cells decide their fates and progress from precursor to progeny, a Johns Hopkins-led team of scientists has produced the first-ever epigenetic landscape map for tissue differentiation. (hopkinsmedicine.org)
Looking into the future: Using induced pluripotent stem cells to build two and three dimensional ocular tissue for cell therapy and disease modeling. (nih.gov)
Furthermore, because they are a renewable source of differentiated cells, these cells are being broadly explored for tissue regeneration. (berkeley.edu)
Spatiotemporal variation in cues that regulate of stem cell function - Stem cells reside within structurally complex tissue microenvironments or niches that present them with numerous control signals including soluble factors, extracellular matrix, and contact-dependent factors from neighboring cells. (berkeley.edu)
In particular, spatial and temporal variation of signals, modulation of tissue mechanical properties, and play critical roles in controlling stem cell function. (berkeley.edu)
We have been applying advances in materials science and bioconjugate chemistry to create synthetic materials that can emulate solid phase properties of natural tissue and - employing an "analysis by synthesis" approach - enable investigations of their effects on stem cell function. (berkeley.edu)
Because of the lack of tissue available for islet transplantation, new sources of β-cells have been sought for the treatment of type 1 diabetes. (diabetesjournals.org)
The in vitro osteogenic differentiation of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) have been one of the hot topics in bone tissue engineering. (researchsquare.com)
Stem cells are defined as a population of cells that can self-renew through symmetrical mitotic division, form daughter cell lines, and generate a broad range of tissue lineages through terminal differentiation [ 1 , 2 ]. (hindawi.com)
In order to be useful for stem cell research, these cells cannot have differentiated into specific types of tissue, such as bone, cardiac, skin, or nerve. (wheelchairkamikaze.com)
With this precise and effective technique, cell profiles can be identified on site in the original tissue. (nih.gov)
What controls the balance between these types of divisions to maintain stem cells at an appropriate level within a given tissue is not yet well known. (nih.gov)
Due to the inaccessibility of primary human tissue, the Tchieu Lab develops strategies to derive these support cells from human pluripotent stem cells. (cincinnatichildrens.org)

2010 . Efficient generation of germ line transmitting chimeras from C57BL/6N ES cells by aggregation with outbred host embryos. (nih.gov)
At the present time, the production of new cell lines involves destruction of preimplantation embryos at the 100-200 cell (blastocyst) stage. (jci.org)
I am grateful for the opportunity to present the Catholic bishops' concerns about efforts to justify the use of cells from deliberately destroyed human embryos in federally funded research. (usccb.org)
I said then that the goals some wish to serve by killing human embryos for their stem cells can be achieved in other, morally acceptable ways. (usccb.org)
After being cultured, some of these stem cells may have a tendency to come back together and develop as an early embryo.4 Whether the formation of early embryos takes place in such a stem cell culture, and whether it can be prevented, is a scientific question. (usccb.org)
But the embryos in question here are zygotes, clumps of at most a few dozen cells. (wheelchairkamikaze.com)
Early mammalian embryos at the blastocyst stage contain two types of cells - cells of the inner cell mass, and cells of the trophectoderm. (nih.gov)
Previous work with mouse embryos led to the development of a method in 1998 to derive stem cells from the inner cell mass of preimplantation human embryos and to grow human embryonic stem cells (hESCs) in the laboratory. (nih.gov)
Using systematic and unbiased functional screening approaches, we discovered that the Id family of helix-loop-helix proteins is both necessary and sufficient to direct cardiac mesoderm formation in frog embryos and human embryonic stem cells. (xenbase.org)
P-W′) Endogenous expression of Id1, Grrp1, Evx1, and Mesp1 in embryonic day 6.5 (E6.5) and E7.25 mouse embryos by in situ hybridization. (xenbase.org)

This work has revealed that not only the overall dosage but also the temporal manner in which these cues are presented impacts cell behavior, results whose underlying molecular mechanisms are being elucidated. (berkeley.edu)
Proficiency in various PCR techniques, including RT-PCR, PCR-RFLP, and molecular cloning, is necessary, along with familiarity with bacterial cell plasmid transformation and transfection methods. (nih.gov)
Multimodal, multiscale imaging modalities will be developed and integrated to understand how molecular level events of key enzymes and protein network are connected to cellular and multi-cellular functions through intra-cellular organization and interactions of the key machineries in the cell. (stanford.edu)

3 Stem Cell Therapies for Neurodegenerative Diseases Lab and National Stem Cell Bank - Valencia Node, Research Center Principe Felipe, Valencia, Spain. (nih.gov)
Development of an inducible caspase-9 safety switch for pluripotent stem cell-based therapies. (nih.gov)
To create next-generation cell therapies, we are also incorporating gene editing with an aim to increase potency and persistence, further reduce immunogenicity, or build safeguard mechanisms into the cells, making cell therapy an even more powerful tool to help patients suffering from intractable diseases. (bayer.com)
Until recently methods of studying muscular disease and potential therapies were dependent on invasive muscle biopsies to produce limited batches of primary cells. (onenucleus.com)
Stem cells are an attractive option in developing cell-based therapies to improve tendon healing. (hindawi.com)
In addition, we are studying the effects of environmental toxicants and targeted cancer therapies on these cells, which may influence patient fertility. (mdanderson.org)

Footprint-free" human induced Pluripotent Stem Cell-derived astrocytes for in vivo cell-based therapy. (wikipedia.org)
12. In Vivo Generation of Engraftable Murine Hematopoietic Stem Cells by Gfi1b, c-Fos, and Gata2 Overexpression within Teratoma. (nih.gov)
Data from in vitro and in vivo models suggest that malnutrition and stress trigger adaptive responses, leading to small for gestational age (SGA) blastocysts with fewer cell numbers. (nih.gov)
Myriad stress inputs for the stem cells in the embryo can occur in vitro during in vitro fertilization/assisted reproductive technology (IVF/ART) or in vivo. (nih.gov)
We studied the expression of SSEA-4 surface protein and the various ESC and MSC markers in the ex vivo cultured limbal stromal cells. (molvis.org)
Participants mentioned the elucidation of factors governing satellite or stem cell survival, distribution, integration, and differentiation in vivo as promising basic research topics. (nih.gov)
The aspiration to utilize such in vitro reprogrammed pluripotent and somatic cells for therapeutic purposes necessitates understanding of the mechanisms of reprogramming and differentiation and elucidating the extent of equivalence of the in vitro derived cells to their in vivo counterparts. (uctv.tv)

In the near future, patient-specific somatic cells could be directly oriented by transdifferentiation into the required cell types. (medscape.com)
On the other hand, genetic abnormalities pre-existing in the somatic cells used for reprogramming or caused by the reprogramming process itself may increase the risk of benign or malignant tumors. (nih.gov)

Eliminating the need for cell sorting or transfection of myogenic transcription factors, the Skeletal Muscle Differentiation kit protocol generates a highly pure population of approximately 70 per cent skeletal muscle myotubes in a reproducible fashion. (onenucleus.com)
For further information on the new Skeletal Muscle Differentiation Kit please visit http://www.amsbio.com/skeletal-muscle-differentiation-kits.aspx or contact AMSBIO on +44-1235-828200 / +1-617-945-5033 / [email protected] . (onenucleus.com)

The areas of emphasis would include systematically profiling and cataloging changes at genetic and epigenetic levels that take place in stem cells and their microenvironment. (nih.gov)
In addition, disease-specific cell line banks, reflecting the genetic and epigenetic variation of the population could be established. (medscape.com)
An understanding of intrinsic and extrinsic signals, especially those involved in the stem cell niche, age-dependent processes and genetic factors that govern the activities of pluripotent cells is crucial in order to utilize them to develop safe and effective treatments for the restoration of function, or to prevent their transformation into tumor-generating cells. (nih.gov)
This cell contains a different set of genetic instructions (resulting in an alternative pattern of gene expression) and is characterized by a reduced proliferative capacity and more restricted developmental potential than its parent. (jci.org)
To achieve this, they used an adapted strategy that consisted of reprogramming cells using a specific "cocktail" of six genetic factors, while erasing signs of aging. (eurekalert.org)
CuSTOM investigators use animal models and state-of-the art single cell genomics to elucidate the cell signaling networks and genetic programs controlling normal embryonic organ development. (cincinnatichildrens.org)

For example, the differentiation of human pluripotent stem cells to cardiomyocytes has been highly inefficient, but here we will described a novel approach using extracellular matrix in combination with growth factors to produce in high yield and purity CMs. (nih.gov)
2007 . Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes. (nih.gov)
Differentiation of pluripotent embryonic stem cells into cardiomyocytes. (bvsalud.org)

For example, it is reported that enamel matrix derivatives (EMD) added at the early stage promote the differentiation of hPSCs into osteoblasts, but it inhibited cell maturation and mineralization at the late stage 12 . (researchsquare.com)

The Tchieu Lab research is focused on investigating how the support cells in our brain (such as astrocytes and microglia) are impacted in neurological disorders. (cincinnatichildrens.org)

Discovering the mechanism behind self-renewal may make it possible to understand how cell fate (stem vs. non-stem) is regulated during normal embryonic development and post-natally, or misregulated as during aging, or even in the development of cancer. (nih.gov)

The MC-813-70 antibody reacts with stage-specific embryonic antigen-4 (SSEA-4), a glycolipid carbohydrate antigen expressed on the surface of human embryonal carcinoma (EC), embryonic germ (EG), undifferentiated embryonic stem (ES) and induced pluripotent stem (iPS) cells, a subset of mesenchymal stem cells, and rhesus monkey ES cell lines. (stemcell.com)
Retinoic acid-induced neural differentiation of P19 embryonal carcinoma cells is potentiated by leukemia inhibitory factor. (muni.cz)
Neural differentiation of pluripotent mouse embryonal carcinoma cells by retinoic acid - inhibitory effect of serum. (muni.cz)

Here in this work, the synergistic effect of chemical and mechanical signaling in driving cells towards mesoderm germ-layer was investigated. (siu.edu)

As a postdoctoral fellow at NCATS, Jovanovic aims to optimize the protocols for neuronal differentiation of human induced pluripotent/embryonic stem cells and to translate his findings into a better understanding and treatment of neurodegenerative diseases. (nih.gov)
Neuronal cell death causes a plethora of neurodegenerative diseases, including age-related loss of memory and dementias (such as Alzheimer's disease), Parkinson's disease, and amyotrophic lateral sclerosis (ALS). (springer.com)

Thus, there is an emphasis on the need for better treatment methods, including hematopoietic cell re-placement strategies as an alternative source for blood cells. (1library.org)
8. Hematopoietic cell differentiation from embryonic and induced pluripotent stem cells. (nih.gov)
Hematopoietic cell transplantation is a curative option. (bvsalud.org)
OBJECTIVES: We sought to evaluate multilineage engraftment and immune reconstitution after conditioning with CD45-antibody drug conjugate (CD45-ADC) as a single agent in hypomorphic mice with Rag1 mutation treated with congenic and haploidentical hematopoietic cell transplantation. (bvsalud.org)

Here we present methodology for the clonal analysis of hematopoietic stem cell precursors during murine embryonic development. (jove.com)

3. Activated HoxB4-induced hematopoietic stem cells from murine pluripotent stem cells via long-term programming. (nih.gov)
The overall goal of this method is to identify the phenotypic properties and engraftment potential of clonal Hematopoietic Stem Cell or HSC precursors during murine embryonic development. (jove.com)
2005 . The murine Cyp1a1 gene is expressed in a restricted spatial and temporal pattern during embryonic development. (nih.gov)

5. Genome-wide gene expression analyses reveal unique cellular characteristics related to the amenability of HPC/HSCs into high-quality induced pluripotent stem cells. (nih.gov)
Signature gene expression during organoid differentiation were assessed by qPCR and immunocytochemistry after COCO supplementation. (biomedcentral.com)
Ubiquitin is a small protein modifier that is ubiquitously produced in the cells and takes part in the regulation of a wide range of cellular activities such as gene transcription and protein turnover. (stanford.edu)
Gene networks in human embryonic stem cells (hESCs) have been found to serve two purposes at once. (genengnews.com)
To address these problems, we used zinc finger nucleases to target a suicide gene, HSV1-TK, into the Oct4 locus, whose promoter is active in undifferentiated cells only. (nih.gov)
According to the results, the developed inkjet bioprinter exhibited cell-friendly dispensing performance, which was similar to the manual dispensing operation, based not only on cell viability but also on gene expression levels. (nature.com)
After differentiation for varying days (0, 7, 14, 21 and 28), we investigated the expression of related gene/protein markers and the differentiation efficiency by RT-PCR, immunofluorescence and alizarin red staining assay. (researchsquare.com)
Moreover, peptide addition could apparently up-regulate the expression of marker gene/protein in hESCs, especially the differentiation efficiency was improved by determining the optimal treatment periods. (researchsquare.com)
Gene therapy (GT) to modify autologous CD34+ cells is an emerging alternative treatment with advantages over standard allogeneic hematopoietic stem cell transplant for patients who lack well-matched donors, avoiding graft-versus-host-disease. (bvsalud.org)

As a prelude to understanding the complexities associated with synaptic wiring, we are especially focusing on photoreceptor pre-synapse morphogenesis and connectivity to horizontal and bipolar cells. (nih.gov)
COCO, a multifunctional antagonist of the Wnt, TGF-β, and BMP pathways, was employed to 3D organoid differentiation schemes for enhanced photoreceptor precursor cells. (biomedcentral.com)
Using COCO as an auxiliary supplement, rather than alone, can yield an increased number of photoreceptor precursors in the early stage of organoid differentiation. (biomedcentral.com)
Therefore, improving the differentiation efficiency of photoreceptor precursor cells during retinalization to supply an unlimited source for cell transplantation will undoubtedly provide valuable applications in the future. (biomedcentral.com)
However, it is unknown whether an earlier stage of intervention during stem cell differentiation would achieve more effective production of photoreceptor precursor cells. (biomedcentral.com)

Found nearly exclusively in pluripotent cells, this protein can also serve as a transcriptional repressor. (nih.gov)

Projects that address comparisons between different classes of human stem cells and between human and non-human stem cells would also be directly relevant to this PA. (nih.gov)
Recently, a bone forming peptide-1 (BFP-1) that derived from bone morphogenetic protein-7 (BMP-7) has confirmed its excellent performance in promoting the osteogenic induction of human stem cells mainly human mesenchymal stem cells. (researchsquare.com)

hiPSC: Human induced pluripotent stem cell. (medscape.com)
Human Induced Pluripotent Stem Cells (hiPSC) are very similar to human embryonic stem cells and have great potential for clinical applications. (nih.gov)

Hematopoietic development is spatiotemporally tightly regulated by defined cell-intrinsic and extrinsic modifiers. (haematologica.org)
1 While embryonic macrophages and nucleated red blood cells are generated early in development in the first wave of primitive hematopoiesis, lymphoid cells, definitive erythro-myeloid progenitors and long-term repopulating HSC are only generated at later stages by the definitive hematopoietic program. (haematologica.org)
An important additional potential in these approaches is to make medicine more personalized by using truly individual human cell preparations in the drug development and toxicity screens. (medscape.com)
The team chose the blood cell system as its model because it's well-understood in terms of cellular development. (hopkinsmedicine.org)
Finally, they looked at older cells that were close to their ultimate fates to get more complete pictures of the precursor-progeny relationships - for example, at white blood cells that had gone fairly far in T-cell lymphocyte development. (hopkinsmedicine.org)
Instead, we saw these waves of change during the development of these cell types. (hopkinsmedicine.org)
The myeloid cells, on the other hand, undergo a wave of increased methylation early in development and then erase that methylation later in development. (hopkinsmedicine.org)
Because the data seem to indicate discreet stages of cell differentiation characterized by waves of changes in one direction and subsequent waves in another, cell types conceivably could be redefined according to epigenetic marks that will provide new insights into both normal development and disease processes. (hopkinsmedicine.org)
This method can help answer key questions in the field of developmental hematopoiesis about the unique lineage contributions of clonal HSC precursors during embryonic development. (jove.com)
While skeletal muscle plays a critical role in human health, the direct study of human skeletal muscle development has been hindered due to the lack of suitable cell-based platforms. (mskcc.org)
Chan and his research team next plan to determine if dental pulp stem cells from monkeys with Huntington's disease can enhance brain cell development in the same way dental pulp stem cells from healthy monkeys do. (sciencedaily.com)
In the field of drug discovery, high-throughput screening of candidate drugs using cell microarrays 6 is expected to contribute to the reduction of costs for the development of new drugs. (nature.com)
It also answers the long-standing question posed by developmental biologists on how the neural crest, which originates from the ectoderm during embryonic development, has such an exceptionally high stem cell potential. (nih.gov)
The findings also indicate a much higher plasticity in the cell fate determination process that occurs in the ectoderm than previously thought," says Dr. Laura Kerosuo , principal investigator of the research group, a former Academy researcher at the University of Helsinki, and currently the Chief of the Neural Crest Development and Disease Unit at the NIH. (nih.gov)
We are particularly interested in the influence of the cellular microenvironment on the fate of these cells during development and disease. (mdanderson.org)
Conversely, specific deletion of RUNX1c dramatically reduced the generation of hematopoietic cells from HEPs, indicating that RUNX1c is a master regulator of human hematopoietic development. (researcher-app.com)
Investigating the mechanisms of self-organizing hepatogenesis at the single cell level is providing new insight into human liver development. (cincinnatichildrens.org)
Abnormal development of mouse embryoid bodies lacking p27Kip1 cell cycle regulator. (muni.cz)

In addition to identifying previously unknown phosphorylation sites on factors associated with differentiation, such as kinases and transcription factors, we observed dynamic phosphorylation of DNA methyltransferases (DNMTs). (nih.gov)
4. Generating autologous hematopoietic cells from human-induced pluripotent stem cells through ectopic expression of transcription factors. (nih.gov)
We propose to use innovative technologies and approaches to define the spatial organization regulating autoimmune T cell differentiation, and test whether modulation of transcription factors associated with the stem-like T cell state disrupts stemness and differentiation, thereby preventing type 1 diabetes. (mskcc.org)
After passaged, a subpopulation of these cells showed low expression of SSEA-4 but were negative for other important ESC surface markers such as Tra-1-60, Tra-1-81, and transcription factors like octamer-binding transcription factor 4 (Oct4), SRY(sex determining region Y)-box 2 (Sox2), and Nanog. (molvis.org)

Because of lacking ABO blood group expression in B-hiPSCs, it has been highlighted as a valuable source to produce any cell type in vitro . (1library.org)
10. Challenges in Cell Fate Acquisition to Scid-Repopulating Activity from Hemogenic Endothelium of hiPSCs Derived from AML Patients Using Forced Transcription Factor Expression. (nih.gov)
Biocompatible BFP1 peptide could remarkably promote the generation of mesoderm cells and mesenchymal-like cells from hiPSCs through the enhanced epithelial-mesenchymal transition, but the effect was not found in hESCs because of cell line difference. (researchsquare.com)
ALS patient-derived human-induced pluripotent stem cells (ALS hiPSCs, harboring the SOD1 AV4 mutation), were differentiated to MNs (ALS-MNs). (springer.com)

Type 1 diabetes results from the breakdown of tolerance mechanisms in β-cell-specific T cells, but many aspects remain enigmatic, including where and how autoimmune β-cell-specific T cells arise and are maintained. (mskcc.org)

Of these markers, we found the greatest increase in expression of the pan glial cell marker, GFAP. (frontiersin.org)
Expression of SSEA-4 is down-regulated following differentiation of human EC, ES, and iPS cells. (stemcell.com)
In contrast, the differentiation of mouse EC, ES, or iPS cells may be accompanied by an increase in SSEA-4 expression. (stemcell.com)
However, little is known about the expression of stage-specific embryonic antigen 4 (SSEA-4) and the embryonic stem cell (ESC)-like properties of these cells. (molvis.org)
Isolated cells were expanded and reanalyzed for their expression of SSEA-4. (molvis.org)
Expression of MSC and ESC markers on these cells were also analyzed by FACS. (molvis.org)
Our study confirmed the expression of SSEA-4 by a subpopulation of cultured limbal stromal cells. (molvis.org)
However, despite the expression of SSEA-4, these cells did not express any other markers of ESC. (molvis.org)
L-O)Temporal expression profiles of Id1, Grrp1, Evx1, and Mesp1 in response to siAcvr1b or siControl from day 3 to day 6 of differentiation. (xenbase.org)
Constitutive expression of RUNX1c in human embryonic stem cells enhanced the appearance of HEPs, including hemogenic (CD43+) HEPs and promoted subsequent differentiation into blood cells. (researcher-app.com)
Expression and potential role of fibroblast growth factor 2 and its receptors in human embryonic stem cells. (muni.cz)
Increased expression of fibroblast growth factor receptor 3 in CD34+ BCR-ABL+ cells from patients with chronic myeloid leukemia. (muni.cz)

Induction of MSCs into specific cell lineages such as tenocytes is determined by culturing processes as well as growth and media conditions (Figure 1 ). (hindawi.com)

The final section will describe a unique cardiac progenitor cell population that we have isolated using the SRF-cardiac specific enhancer which may prove useful in cardiac repair. (nih.gov)
Well, seems a patient heart attacked by any disease and his cardiac cells going to be dysfunctional. (omicsonline.org)
However, the control of directing pluripotent cells into mesoderm lineage which gives rise to cardiac muscle cells remains poor. (siu.edu)
Future studies with human pluripotent stem cells may transduce cells towards mesoderm and finally towards cardiac fate. (siu.edu)
Mechanistically, Id proteins specify cardiac cell fate by repressing two inhibitors of cardiogenic mesoderm formation- Tcf3 and Foxa2 -and activating inducers Evx1 , Grrp1, and Mesp1 . (xenbase.org)

In order to engineer an environment supporting bone formation, combinations of biochemical and biophysical signals need to be presented to the cells in a three-dimensional setting in a way that allows cellular interactions with the surrounding cells and extracellular matrix (Figure 1 , left). (biomedcentral.com)
Particularly, we are uncovering the importance of extracellular matrix molecules, and of neuroimmune cells in repair and regeneration processes after injury to the central nervous system. (uw.edu)
We are also interested the effects of these two important factors (neuroimmune cells and extracellular matrix) on the differentiation of both embryonic and induced pluripotent stem cells. (uw.edu)

Anatomy54

Organisms10

Diseases5

Chemicals and Drugs75

Analytical, Diagnostic and Therapeutic Techniques and Equipment47

Phenomena and Processes66

Disciplines and Occupations5

Technology, Industry, Agriculture1

Information Science3

IPSCs21

Mouse embryonic stem7

Tissues19

Pluripotency13

ESCs7

Adult31

Generated from human pluripotent stem1

Progenitor cells9

Regenerative medicine11

Neurons8

Differentiate13

HESC4

Early embryonic development3

Proliferation5

Genes14

Neural stem1

Characteristics of embryonic1

IPSC6

Biology14

Derivation3

Characterization3

Endothelial7

Retinal14

Mesenchymal cells1

PSCs5

Vitro differentiation2

Organoids5

Cellular differentiation2

Multipotent3

Blastocyst-derived2

Tissue15

Embryos10

Molecular3

Therapies6

Vivo7

Somatic cells2

Skeletal Muscle Differentiation2

Genetic6

Cardiomyocytes3

Promote the differentiation1

Astrocytes1

Normal embryonic1

Embryonal carcinoma3

Germ-layer1

Neuronal2

Hematopoietic cell4

Analysis of hematopoietic1

Murine3

Gene9

Photoreceptor5

Transcriptional1

Human stem cells2

HiPSC2

Development18

Transcription Factors4

HiPSCs4

Mechanisms1

Expression12

Lineages1

Cardiac5

Extracellular3