• A good example would be a mesenchymal stem cell which can develop into bone, muscle and cartilage cells. (progencell.com)
  • This review provides a brief history of mesenchymal stem cell (MSCs) and provides an updated view of the many stem/progenitor cell populations contributing to fracture repair. (researchgate.net)
  • Specifically, about the paracrine activities of amniotic fluid stem cells, renal stem cells, embryonic stem cells, mesenchymal stem cell, induced pluripotent stem cells as well as other stem cells. (biomedcentral.com)
  • Among these, adipose-derived stem cells (ADSCs) are a type of mesenchymal stem cell isolated from adipose tissue, which has the advantages of abundant storage in vivo , easy acquisition, and expansion [ 13 - 15 ]. (hindawi.com)
  • The aims of this article are to review the current knowledge regarding therapeutic mechanisms of mesenchymal stem cells in acute liver failure, to discuss recent advancements in preclinical and clinical studies in the treatment of mesenchymal stem cells, and to summarize the methodological improvement of mesenchymal stem cell transplantation in treating liver failure. (springer.com)
  • WAT is characterised by its capacity to adapt and expand in response to surplus energy through processes of adipocyte hypertrophy and/or recruitment and proliferation of precursor cells in combination with vascular and extracellular matrix remodelling. (springer.com)
  • There is a growing body of evidence which demonstrates that following ACS, microRNAs might inhibit fibroblast proliferation and scarring, as well as harmful apoptosis of cardiomyocytes, and stimulate fibroblast reprogramming into induced cardiac progenitor cells. (archivesofmedicalscience.com)
  • Most of these paracrine secretions include soluble factors and exosomes, which regulate the repair and regeneration processes at sites of damage by affecting cell proliferation, migration, and differentiation [ 22 , 23 ]. (hindawi.com)
  • We are now using the iPS cell technology after generating iPS cell lines for some of these patients and performing studies of patient specific disease modeling on the study of cardiomyocyte formation and most importantly, proliferation. (unl.pt)
  • The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
  • All- trans retinoic acid regulates proliferation, migration, differentiation, and extracellular matrix turnover of human arterial smooth muscle cells. (zju.edu.cn)
  • Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli. (zju.edu.cn)
  • Likewise, they have the ability to activate the angiogenesis, proliferation, migration, and differentiation of the main cell types involved in skin regeneration. (frontiersin.org)
  • Since mesenchymal stem cells (MSCs) have effective immune-modulatory features, inhibit T-lymphocyte proliferation, and have a high regenerative capacity, they can play a vital role in the reconstruction of injured tissues, including bronchioles, in pulmonary diseases. (biomedcentral.com)
  • The latter findings point to the participation of Nogo-A/NgR1 signaling in the regulation of other aspects of growth, such as tissue expansion or turnover by cell proliferation. (jneurosci.org)
  • In both processes, rapid proliferation gives rise to new tissue, cell fate has to be specified within that tissue, and distinct positional identities have to be established to generate a properly patterned structure. (frontiersin.org)
  • BMP9 inhibits the proliferation and invasiveness of breast cancer cells MDA-MB-231. (uchicago.edu)
  • Mesenchymal stem cells (MSCs) are multipotent cells usually isolated from bone marrow, endometrium, adipose tissues, skin, and dental pulp. (techscience.com)
  • MSCs played a crucial role in regenerative therapy and have been introduced as an interdisciplinary field between cell biology and material science. (techscience.com)
  • However, MSCs have gained considerable attention for tissue regeneration owing to their differentiation ability with immunomodulatory effects. (techscience.com)
  • Mesenchymal stem cells (MSCs) are multipotent stromal cells capable of self-renewal and exhibit multilineage differentiation properties. (techscience.com)
  • The Mesenchymal and Tissue Stem Cell Committee, which belongs to the International Society for Cellular Therapy, stated that plastic-adherent properties are the minimum criteria for determining MSCs. (techscience.com)
  • The most commonly used type of stem cells, mesenchymal stem cells (MSCs), can be easily isolated from bone marrow or synovium and cultured in vitro . (openorthopaedicsjournal.com)
  • These osteoconductive three dimensional constructs seeded with MSCs are highly porous, biodegradable and biomechanically stable scaffolds which do not evoke an immunogenic host cell response. (openorthopaedicsjournal.com)
  • Research has shown the importance of growth factors in guiding and modulating the differentiation of MSCs in order to obtain the required cell type. (openorthopaedicsjournal.com)
  • 6,7 Mesenchymal stem cells (MSCs) have also been isolated from other sources, such as bone marrow, 8 the placenta, 9 muscle, 10 or blood. (jcadonline.com)
  • Over the last decades, mesenchymal stem cells (MSCs) have attracted extensive attention in the field of bone regeneration [ 4 - 7 ]. (hindawi.com)
  • MSCs are a population of nonhematopoietic adult stem cells that have the property of self-renewal and can differentiate into multiple lineages [ 8 - 10 ]. (hindawi.com)
  • With the in-depth researches of MSCs, an increasing number of studies have indicated that the therapeutic effects of MSCs can be attributed not only to their differentiation capacity but also to their paracrine action [ 21 ]. (hindawi.com)
  • Adipose tissue (AT) represents a commonly used source of mesenchymal stem/stromal cells (MSCs) whose proregenerative potential has been widely investigated in multiple clinical trials worldwide. (mdpi.com)
  • Over the last few decades numerous studies have reported the beneficial effects of mesenchymal stem cells (MSCs) on tissue repair and regeneration. (biomedcentral.com)
  • MSCs can not only differentiate into lung alveolar epithelial cells and secrete anti-inflammatory factors, but they also deliver some vehicles for gene therapy in repairing the injured lung, which provides new ideas for managing RILI. (biomedcentral.com)
  • The mechanisms of radiation-induced lung injury (RILI) and the mechanisms by which mesenchymal stem cells (MSCs) alleviate it. (biomedcentral.com)
  • Mesenchymal stem cells (MSCs) are ASCs, and were first described in 1966 by Friedenstein et al. (bvsalud.org)
  • Mesenchymal stem/stromal cells (MSCs) are increasingly used as an intravenously applied cellular therapeutic. (stemcellslab.net)
  • Later, Arnold Caplan's group described mesenchymal stem/stromal cells (MSCs) as multipotent mesenchymal cell populations which can differentiate into several tissue types, and demonstrated roles for MSCs in the regeneration of bone, cartilage or ligaments in animal and clinical studies [2-4]. (stemcellslab.net)
  • Pivotal studies by the group of Horwitz in children with osteogenesis imperfecta, an inherited enzyme deficiency of collagen synthesis by mesenchymal cells in bone, opened the field for intravenous use of MSCs. (stemcellslab.net)
  • The tissue source of the MSCs was in most cases not decisive, and cells from various tissue sources were explored. (stemcellslab.net)
  • and signals from the injected cells were found early after administration of the MSCs at the highest frequencies in the lungs, followed by liver and spleen. (stemcellslab.net)
  • MSCs have the capacity of multiple differentiations and self-renewal and have been proven to be able to differentiate into a series of cell lineages, such as adipocytes, osteoblasts, chondroblasts, and hepatocyte-like cells (HLCs). (springer.com)
  • Different groups have established several protocols to induce the differentiation of MSCs into HLCs in vitro . (springer.com)
  • In practice, stem cells are identified by whether they can regenerate tissue and not according to their potency. (progencell.com)
  • The most common and scientifically accepted type of stem cells used with therapeutic purposes are adult stem cells (also known as somatic stem cells or tissue stem cells) which can be pluripotent, multipotent, oligopotent and/or unipotent stem cells. (progencell.com)
  • 2017, Z. around the emergence of patterns and tissue organization, and information regarding the events occurring at the level of individual cells is only now beginning to emerge. (cancercurehere.com)
  • Nevertheless, research in tissue tradition have shown that whenever induced by exterior hormone software, callus originates particularly from specific pericycle-like cells discovered throughout the vegetable (Atta et al. (cancercurehere.com)
  • Enhanced expression of IL-6, IGF-1, TGF-β, and VEGF has also been observed in LC treated BMSCc+ groups, suggested the cardiac differentiation of BMSCc+, and can be utilized in tissue engineering for cardiac cell therapy. (techscience.com)
  • However, it can be challenging to differentiate when they are connected with wounded tissue, opposed to when they instruct tissue-specific progenitor cells responsible for the redevelopment of damaged tissue. (techscience.com)
  • The heart is the first organ to form and has a complex structure consisting of many specialized cell types populating the heart muscle, blood vessels, and connective tissue. (tum.de)
  • As cells cultured in 2D fail to recapitulate certain features of their in vivo counterparts, part of our work is dedicated to developing 3D cardiac models that better mimic the physiology of heart tissue. (tum.de)
  • We routinely produce engineered heart tissue based on hiPSC-derived cardiac cells seeded on decellularized ventricular matrix from pigs or non-human primates, which we cultivate in a biomimetic chamber system promoting tissue maturation by continuous mechanical and electrical stimulation. (tum.de)
  • Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
  • Stem cell therapy is an exciting and upcoming branch of tissue engineering with application in the field of orthopaedics. (openorthopaedicsjournal.com)
  • Initial studies indicate an immense potential for cell based strategies to enhance current orthopaedic approaches in skeletal tissue reconstruction. (openorthopaedicsjournal.com)
  • Nevertheless, stem cell based tissue engineering in orthopaedics shows a promising future. (openorthopaedicsjournal.com)
  • Amidst this, tissue engineering using stem cells has emerged as a hot topic of research and is a promising alternative to the traditional techniques of bone grafting. (openorthopaedicsjournal.com)
  • In this review, we discuss the potential influence of stromal vascular fraction from adipose tissue in the management of alopecia as well as its involvement in preclinical and clinical trials. (jcadonline.com)
  • Adipose tissue is a loose connective tissue composed of cells supported by an intracellular matrix as well as by vascular, lymphatic, and neural networks. (jcadonline.com)
  • Recently, a number of studies have confirmed that ADSCs possess the ability to differentiate into adipocytes, osteoblasts, and chondrocytes [ 16 - 18 ], suggesting that a broader source of stem cells is available for application in tissue engineering. (hindawi.com)
  • Mariano García Arranz has the following conflict of interest: MGA is inventor on 2 patents entitled "Identification and isolation of multipotent cells from non-osteochondral mesenchymal tissue" (10157355957US) and "Use of adipose tissue-derived stromal stem cells in treating fistula" (US11/167061). (wjgnet.com)
  • The precise control of tissue-specificity of gene expression is essential for successful development and depends on distal cis -regulatory elements such as enhancers which interact with promoter elements in physical space. (amegroups.org)
  • Validate the potential of CCBE1 in the formation of new vessels and in the remodeling/repair of injured cardiac tissue both by using 3D cell-based models as well as using mouse models of myocardial infarction. (unl.pt)
  • CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
  • may serve as a potent agent for functional smooth muscle cell differentiation in tissue engineering. (zju.edu.cn)
  • When lung tissue is irradiated by x-ray cells are injured by the direct or indirect action of radiation. (biomedcentral.com)
  • Due to the ability to self-renew and to differentiate into cells that are found throughout the body, there is a great interest in using stem cells for the regeneration of injured tissues as well as to develop tissue-engineered implants and bio-hybrid organs, in order to restore tissue function. (bvsalud.org)
  • The use of ASCs in regenerative medicine and tissue engineering research has important advantages in comparison with ESCs, since there are no ethical complications and the process of differentiation of these cells is better controlled. (bvsalud.org)
  • In explant method, the dental tissue is placed on a plastic surface and the cells migrate out from the tissue fragment adhering to culture flasks or dishes ( Figure 1 ). (bvsalud.org)
  • The acute-phase HGF-treated group exhibited vascularization, diverse anti-inflammatory effects, and activation of endogenous neural stem cells after SCI, which collectively contributed to tissue preservation. (biomedcentral.com)
  • It is well-established that early embryonic signaling pathways are critical for growth and patterning of new tissue during regeneration. (frontiersin.org)
  • The regeneration of a tissue intuitively recapitulates aspects of its embryonic development. (frontiersin.org)
  • Connective tissue growth factor (CTGF) is regulated by Wnt and bone morphogenetic proteins signaling in osteoblast differentiation of mesenchymal stem cells. (uchicago.edu)
  • Stem cells are known to work via a variety of methods to heal tissue depending on where the stem cells come from and into which tissue it is being placed. (drcremers.com)
  • Here is a general list of all the Ways Stem Cells heal tissues or create new tissue. (drcremers.com)
  • 1. Stem cells have long been known to have the ability to differentiate (ie become/transform) into "good/normal" tissue. (drcremers.com)
  • Embryonic stem cells have been fraught with difficulties in coaxing them into "good/normal" tissue and there are major ethical issues. (drcremers.com)
  • Here, I review the historical and current ideas of cell identification and identification transitions, and discuss how new tools and sights may instruct the near future knowledge of differentiation and vegetable regeneration. (cancercurehere.com)
  • 1958). This means that that as the pericycle, using its putative specific properties, may be the primary contributor to cells culture-based regeneration, pluripotency could be wide-spread amongst vegetable cells. (cancercurehere.com)
  • Recent advances in developmental and stem cell biology have paved the way for a wide spectrum of novel cardiovascular therapies, from the transplantation of stem cell-derived cells to the stimulation of endogenous regeneration processes. (tum.de)
  • These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
  • The expression of Prx1 has been used as a marker to define the skeletal stem cells (SSCs) populations found within the bone marrow and periosteum that contribute to bone regeneration. (researchgate.net)
  • This review considers the genetic and epigenetic control of nephrogenesis, together with the epigenetic mechanisms that accompany kidney development and recent advances in induced reprogramming and kidney cell regeneration in the context of DN. (emjreviews.com)
  • Make noticeable progress in pluripotent stem cell-derived cardiomyocyte maturity toward a more clinically relevant model for cardiac regeneration. (unl.pt)
  • These cells have been studied for the treatment of periodontitis, bone repair, regeneration of the pulp after necrosis as well as the development of new teeth. (bvsalud.org)
  • Following cell transplantation into a favorable environment, the transplanted NS/PCs survived well, facilitating remyelination and neuronal regeneration in host tissues. (biomedcentral.com)
  • however, a variety of other cell types are found as well, collectively labeled as stromal vascular fraction (SVF). (jcadonline.com)
  • The mesenchymal stromal cells (MSC) have immunomodulatory properties and a high regenerative capacity. (frontiersin.org)
  • This concept started from the observation that bone marrow transplantation can provide stromal cells able to synthesize intact collagen type I, replacing deficient patient cell function and ameliorating disease symptoms [5]. (stemcellslab.net)
  • Both ES cells and iPS cells are pluripotent stem cells with capabilities of indefinite self-renewal and can be differentiated into almost all cell types of the body, which make them valuable for studying early developmental biology, for modeling and as therapy for human diseases. (biomedcentral.com)
  • The self-renewal and proliferative nature of stem cells raised the hope to fight against various diseases. (biomedcentral.com)
  • Stem cells (SCs) are undifferentiated cells with self-renewal ability and capacity to differentiate into specialized cell types. (bvsalud.org)
  • To explore VHL as a regulator defining nephron progenitor self-renewal versus differentiation, we bred Six2-TGC tg mice with VHL lox/lox mice to generate mice with a conditional deletion of VHL from Six2 + nephron progenitors. (lww.com)
  • One of our areas of interest is the role of transcription factors such as Isl1, which during heart development is expressed in so-called second heart field (SHF) cardiovascular progenitors that later mainly contribute to the right ventricle, the outflow tract and portions of the atria. (tum.de)
  • Whole - mount immunohistological staining of an Isl1Cre/+;R26mTmG/+ embryo at embryonic day 9.5 show ing Isl1-Cre mediated mG (green) marking of SHF progenitors and their derivatives. (tum.de)
  • Besides its role in coronary vasculature formation, we demonstrated that CCBE1 is required for cardiac mesoderm formation and multipotent cardiovascular progenitors' differentiation in mouse ESCs. (unl.pt)
  • Nephron progenitors, the self-renewing cells that give rise to nephrons, are particularly metabolically active, relying primarily on glycolysis for energy generation early in development. (lww.com)
  • As development progresses, nephron progenitors switch from glycolysis to mitochondrial respiration for energy-mediated by an unknown mechanism-and undergo differentiation. (lww.com)
  • In addition to identifying VHL as a critical regulator of nephron progenitors' metabolic switching, the authors' findings demonstrate that this switch also plays a large role in the differentiation process, and suggest that VHL is required for normal kidney development. (lww.com)
  • Nephron progenitors, the cell population that give rise to the functional unit of the kidney, are metabolically active and self-renew under glycolytic conditions. (lww.com)
  • By embryonic day 15.5, kidneys of nephron progenitor cell-specific VHL knockout mice begin to exhibit reduced maturation of nephron progenitors. (lww.com)
  • BMP-9-induced osteogenic differentiation of mesenchymal progenitors requires functional canonical Wnt/beta-catenin signalling. (uchicago.edu)
  • Conditional immortalization establishes a repertoire of mouse melanocyte progenitors with distinct melanogenic differentiation potential. (uchicago.edu)
  • citation needed] Since iPSCs can be derived directly from adult tissues, they not only bypass the need for embryos, but can be made in a patient-matched manner, which means that each individual could have their own pluripotent stem cell line. (wikipedia.org)
  • It can give rise to embryonic as well as extraembryonic tissues. (progencell.com)
  • It's possible that one cell types, just like the pericycle, are primed and may quickly acquire pluripotency currently, while cells from additional tissues have to go through a competence acquisition stage before their pluripotent potential turns into apparent. (cancercurehere.com)
  • hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
  • Prx1 expression also connotes calvaria and appendicular tissues during embryonic develop. (researchgate.net)
  • It has been found that some of the SVF cell types possess regenerative and anti-inflammatory potentials in damaged tissues due to their ability to secrete growth factors and anti-inflammatory molecules. (jcadonline.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • In human postnatal dental tissues, five main sources of DSCs have been identified: dental pulp stem cells (DPSCs), 11 stem cells from human exfoliated deciduous teeth (SHEDs), 12 periodontal ligament stem cells (PDLSCs), 13 dental follicle stem cells (DFSCs) 14 and stem cells from apical papilla (SCAPs). (bvsalud.org)
  • CD140b is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. (thermofisher.com)
  • Pluripotent stem cells hold promise in the field of regenerative medicine. (wikipedia.org)
  • Stem Cells Applications in Regenerative Medicine and Disease Therapeutics. (progencell.com)
  • We are particularly interested in how these processes are disrupted in congenital heart defects and how we can use knowledge from development to develop new regenerative approaches in adults. (tum.de)
  • Cartilage heals very poorly, owing to the lack of intrinsic regenerative capacity of the chondrocytes and its poor vascular supply. (openorthopaedicsjournal.com)
  • Understanding their mechanisms may lead to the development of microRNA cocktails that can potentially be used in regenerative cardiology. (archivesofmedicalscience.com)
  • It includes the possible stem cell therapy mechanisms involved and outcomes recorded so far, the limitations of using these regenerative medicines, and the progressive improvement in stem cell therapy by adopting approaches like PiggyBac, Sleeping Beauty, and the Sendai virus. (biomedcentral.com)
  • As a stem cell and developmental molecular biologist, Dr. Fame is dedicated to broad collaboration focused on translating an understanding of neural development and CSF biology into regenerative strategies for the treatment of neurodevelopmental disease. (stanford.edu)
  • bone marrow-derived mesenchymal stem cells are multipotent stem cells, an attractive resource for regenerative medicine. (zju.edu.cn)
  • Therefore, research on exosomes produced by MSC has been intensified for use in cell-free regenerative medicine. (frontiersin.org)
  • Conclusion: stem cells from dental origin are an interesting alternative for research and application in regenerative therapies in Dentistry. (bvsalud.org)
  • 8,9 Because dental stem cells (DSCs) are easy to obtain and present a great potential of differentiation, there has been a growing interest in their use in regenerative medicine for treatment of various human diseases. (bvsalud.org)
  • A totipotent stem cell has the ability to differentiate into any type of cell in an organism. (progencell.com)
  • Oligopotent stem cells have the ability to differentiate into a few cell types. (progencell.com)
  • Mouse ES cells have been widely utilized as an in vitro model to study cardiogenesis, as cardiomyocytes were found to spontaneously differentiate from ES cells after withdrawal of LIF (leukemia inhibitory factor), which functions to maintain the pluripotency of undifferentiated mouse ES cells [ 2 - 4 ]. (biomedcentral.com)
  • During early cardiac development, cells must multiply rapidly but also specify, i.e. limit their developmental potential and determine into which cell type they will later differentiate. (tum.de)
  • Our group investigates how different cardiovascular progenitor populations arise, specify, and differentiate, notably by dissecting lineage trajectories via single-cell transcriptomics and chromatin accessibility profiling. (tum.de)
  • Intradermal adipocyte progenitor cells are activated toward the end of the telogen phase to proliferate and differentiate into mature adipocytes. (jcadonline.com)
  • Mesenchymal progenitor cells differentiate into an endothelial phenotype, enhance vascular density, and improve heart function in a rat cellular cardiomyoplasty model. (zju.edu.cn)
  • The differentiated ES cell cultures are heterogeneous and contain undifferentiated ES cells, which could result in teratoma formation after transplantation into the host. (biomedcentral.com)
  • His research focus is on haematopoietic stem cell transplantation (HSCT) and translational research of normal and malignant stem cells. (edu.au)
  • Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
  • Human induced pluripotent stem cell-derived neural stem/progenitor cell (hiPSC-NS/PC)-based cell transplantation has emerged as a groundbreaking method for replacing damaged neural cells and stimulating functional recovery, but its efficacy is strongly influenced by the state of the injured spinal microenvironment. (biomedcentral.com)
  • Therefore, the development of a strategy that improves the environment of the injured spinal cord to enhance the efficacy of NS/PC transplantation is crucial. (biomedcentral.com)
  • In these studies, however, transplanted cells were followed, if at all, at the site of transplantation, and biodistribution was not an issue. (stemcellslab.net)
  • Transduced bovine articular chondrocytes affect the metabolism of cocultured nucleus pulposus cells in vitro: implications for chondrocyte transplantation into the intervertebral disc. (uchicago.edu)
  • The most efficient method to expand limbal stem cells (LSCs) in vitro for clinical transplantation is to culture single LSCs directly on growth-arrested mouse fibroblast 3T3 cells. (drcremers.com)
  • Surfactant is produced by alveolar type II cells which can be differentiated in vitro from patient specific induced pluripotent stem cell (iPSC)-derived lung organoids. (nature.com)
  • Gene therapy has been utilized successfully to repair or inactivate mutations in animal models of monogenic human diseases 19 as well as human in vitro cells 12 . (nature.com)
  • Un rôle majeur des prostaglandines dans l'hématopoïèse, ainsi mis en évidence, a pu être étendu à la souris chez qui un traitement par la prostaglandine PGE2 provoque la multiplication des CSH in vivo et in vitro, un résultat qui génerera très probablement des applications cliniques. (hypotheses.org)
  • In the research projects outlined below, we combine 2D and 3D in vitro models based on human pluripotent stem cells with animal models such as the mouse and pig. (tum.de)
  • They have the capacity to self-renew indefinitely and to give rise to any cell type of the body in vitro , making them an exceptionally powerful tool for studying human disease in patient-specific backgrounds. (tum.de)
  • A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
  • Subsequent in vitro and in vivo studies have demonstrated the initial steps of cancer cell invasion and dissemination by disrupting the integrity of the myoepithelial belt that encircles the LECs [ 7 , 8 , 9 , 10 , 11 ]. (biomedcentral.com)
  • To reduce possible xenobiotic contamination from 3T3s, primary human adipose-derived stem cells (ASCs) were examined as feeder cells to support the expansion of LSCs in vitro . (drcremers.com)
  • dubious - discuss] In his Nobel seminar, Yamanaka cited the earlier seminal work of Harold Weintraub on the role of myoblast determination protein 1 (MyoD) in reprogramming cell fate to a muscle lineage as an important precursor to the discovery of iPSCs. (wikipedia.org)
  • A unipotent stem cell differentiates along a single lineage of cells. (progencell.com)
  • Also making up a part of the hematopoietic system are short-term repopulating progenitor cells, which give rise to lineage-specific cell types. (stemcell.com)
  • Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
  • Over the past decades, luminal epithelial cell lineage has gained considerable attraction as the functionally milk-secreting units and as the most fruitful acreage for breast cancer launching. (biomedcentral.com)
  • Recognition of the effective involvement of the myoepithelial cells in mammary gland development and in hampering tumorigenesis has renewed the interest in investigating the biological roles of this second main mammary lineage. (biomedcentral.com)
  • RNA interference-mediated gene silencing of vascular endothelial growth factor in colon cancer cells. (uchicago.edu)
  • Her research program encompasses the early neural stem cell niche, neural tube closure, cerebrospinal fluid (CSF), metabolism, and cortical neuronal development. (stanford.edu)
  • Our research program encompasses the early neural stem cell niche, neural tube closure, CSF, metabolism, and cortical neuronal development. (stanford.edu)
  • The different expression of isoforms H, M and L of synemin in the nervous system raises several questions about the regulation of synemin gene expression during the determination of glial and neuronal cell lineages in the central and the peripheral nervous system (CNS and PNS). (biomedcentral.com)
  • You can record from neuronal or cardiac cultures, stem cells, or brain or cardiac slices. (multichannelsystems.com)
  • 3 Embrionic stem cells (ESCs) are derived from the inner cell mass of the blastocyst and form all cell types, derived from the three germ layers, and are therefore pluripotent. (bvsalud.org)
  • In this study, we verified that exosomes derived from osteogenically differentiated ADSCs can promote osteogenic differentiation of ADSCs. (hindawi.com)
  • Moreover, we found that these differentially expressed exosomal miRNAs connect osteogenic differentiation to processes such as axon guidance, MAPK signaling, and Wnt signaling. (hindawi.com)
  • This study confirms that alterations in the expression of exosomal miRNAs can promote osteogenic differentiation of ADSCs, which also provides the foundation for further research on the regulatory functions of exosomal miRNAs in the context of ADSC osteogenesis. (hindawi.com)
  • Hey1 basic helix-loop-helix protein plays an important role in mediating BMP9-induced osteogenic differentiation of mesenchymal progenitor cells. (uchicago.edu)
  • Retinoic acids potentiate BMP9-induced osteogenic differentiation of mesenchymal progenitor cells. (uchicago.edu)
  • Distinct roles of bone morphogenetic proteins in osteogenic differentiation of mesenchymal stem cells. (uchicago.edu)
  • Insulin-like growth factor 2 (IGF-2) potentiates BMP-9-induced osteogenic differentiation and bone formation. (uchicago.edu)
  • Biphasic effects of TGFß1 on BMP9-induced osteogenic differentiation of mesenchymal stem cells. (uchicago.edu)
  • BMP-9 induced osteogenic differentiation of mesenchymal stem cells: molecular mechanism and therapeutic potential. (uchicago.edu)
  • A comprehensive analysis of the dual roles of BMPs in regulating adipogenic and osteogenic differentiation of mesenchymal progenitor cells. (uchicago.edu)
  • However, since the generation of embryonic stem cells involves destruction (or at least manipulation) of the pre-implantation stage embryo, there has been much controversy surrounding their use. (wikipedia.org)
  • We have also established an ex vivo embryo culture system that allows us to study the function of human cardiac cells in human-mouse or human-pig chimeras. (tum.de)
  • In quick succession, the preimplantational mouse embryo undergoes a series of cellular divisions involving two critical cell fate decisions. (zeiss.com)
  • During the transition from the naive epiblast to a pluripotent state, over 100 cells are organized into an approximately 60-80 µm diameter pre-implantation mouse embryo. (zeiss.com)
  • In the developing embryo, TFs regulating the assembly/disassembly of transcriptional complexes and ultimately gene expression, are directed by complex extrinsic signalling processes which connect all cells within a multi-cellular organism to their environment. (amegroups.org)
  • 3,4 The zygote and cells derived from the first two cellular divisions constitute the most primitive cells (totipotent cells) that are capable of forming the embryo and the embryonic annexes (e.g. placenta, amniotic membranes etc). (bvsalud.org)
  • Here we show the differentiation of patient specific iPSCs derived from a patient with SFTPB deficiency into lung organoids with mesenchymal and epithelial cell populations from both the proximal and distal portions of the human lung. (nature.com)
  • Lung development is a complicated process that involves the specialization of multiple bronchiolar and alveolar epithelial populations. (nature.com)
  • Although many differentiation protocols in the literature have been successful in mimicking lung development from stem cells, there has not been an examination of how a specific mutation impacts the differentiation process including its effects on the early endoderm, as well as the proximal and distal lung epithelial cell populations in the lung organoids. (nature.com)
  • Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
  • In the 1970s, Friedenstein and colleagues [1] first reported that locally applied culture-expanded populations of bone marrow stroma-derived fibroblastic cells remained at their injection sites under the kidney capsule, where an ectopic hematopoiesis was initiated. (stemcellslab.net)
  • We have examined the expression profile of synemin isoforms in mouse pluripotent ES cells and during their neural differentiation induced by retinoic acid. (biomedcentral.com)
  • Synemin H was produced only in neural precursors when neural differentiation started, concurrently with synemin M, nestin and glial fibrillary acidic protein. (biomedcentral.com)
  • However, both synemin H and M were restricted to the progenitor line during the neural differentiation program. (biomedcentral.com)
  • Our study shows a developmental stage specific regulation of synemin isoforms in ES cells and its neural derivatives. (biomedcentral.com)
  • These findings represent the first evidence that synemins could potentially be useful markers for distinguishing multipotent ES cells from undifferentiated neural stem cells and more committed progenitor cells. (biomedcentral.com)
  • Alveolar type II (ATII) epithelial cells synthesize, secrete and recycle all components of surfactant and dysfunction in surfactant metabolism can result in a variety of pediatric lung diseases including respiratory distress syndrome and interstitial lung disease 3 . (nature.com)
  • Alveolar epithelial cells participate in the pathogenesis of fibrosis, producing pro-inflammatory mediators, and undergoing epithelial-to-mesenchymal transition (EMT). (biomedcentral.com)
  • The ductal system is coated by a chain of luminal epithelial cells (LECs) situated on a layer of myoepithelial cells (MECs) and encompassed by a distinguished basement membrane. (biomedcentral.com)
  • Two major cell types cooperate to build up the ductal units: inner polarized luminal epithelial cells (LECs) embraced by a layer of myoepithelial cells (MECs), both cell types are rimmed by a cohesive basement membrane (BM) [ 2 ]. (biomedcentral.com)
  • Therefore, damaging the MECs layer resulted in the release of various factors (such as SDF1/CXCL12, CXCL14, MMP, and tenascin) [ 11 , 12 ] with a potential to modify the tumour microenvironment and facilitate the paracrine communication between the tumour epithelial cells and the enclosed stroma enhancing the tumour aggressiveness [ 11 , 13 ]. (biomedcentral.com)
  • He has delineated the function of these molecules in diverse set of biological processes including: 1) gut and kidney formation, 2) vascular permeability, 3) mucosal inflammatory disease, 4) stem cell homing and migration, and 5) epithelial tumor progression. (ubc.ca)
  • Crosstalk between NF-kappaB and beta-catenin pathways in bacterial-colonized intestinal epithelial cells. (uchicago.edu)
  • To optimize the ASC-supported culture, freshly isolated limbal epithelial cells in the form of single cells (SC-ASC) or cell clusters (CC-ASC) were cultured using three different methods: LSCs seeded directly on feeder cells, a 3-dimensional (3D) culture system and a 3D culture system with fibrin (fibrin 3D). (drcremers.com)
  • CC-ASC generated epithelial cells with undifferentiated morphology in all culture methods, among which CC-ASC in 3D culture supported the highest cell doubling (cells doubled 9.0 times compared to cells doubled 4.9 times in control) while maintained the percentage of putative limbal stem/progenitor cells compared to the control. (drcremers.com)
  • Commensurate with the known truth that HuR improved the great quantity of adhesion substances VLA-4 on Th17 cells, knockout of HuR impaired splenic Th17 cell migration towards the central anxious program and abolished the condition. (globaltechbiz.com)
  • Through them, the cells modify their microenvironment and the behavior of neighboring cells. (frontiersin.org)
  • His laboratory has followed two primary interests: 1) the transcription factor networks that regulate fate determination in various cells that make blood, and 2) the cell surface proteins expressed by hematopoietic stem cells that and allow them to communicate with their microenvironment. (ubc.ca)
  • 4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
  • Stem Cell Research Advances , edn. (edu.au)
  • All- trans retinoic acid promotes smooth muscle cell differentiation of rabbit bone marrow-derived mesenchymal stem cells[J]. Journal of Zhejiang University Science B, 2010, 11(7): 489-496. (zju.edu.cn)
  • Embryonic stem cells isolation primarily requires the consent of donors and can include the killing of fertilized eggs. (techscience.com)
  • Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
  • This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)
  • Vasculogenic mimicry (VM) is a blood supply modality that occurs independently of endothelial cell angiogenesis. (oncotarget.com)
  • Understanding how embryonic cells biomolecularly restrict their early developmental potential and promote specific cell commitment helps to provide key insights on cancer plasticity and could improve fertilization protocols. (zeiss.com)
  • However, all developmental processes originate from pluripotent precursor cell types. (amegroups.org)
  • How developmental trajectories are encoded in the genome and how the interplay between intrinsic and extrinsic processes regulates development is one of the major questions in developmental biology. (amegroups.org)
  • We also highlight recent studies identifying the cis -regulatory elements such as enhancers at the global level and explain how their developmental activity is regulated by the cooperation of cell-type specific and ubiquitous transcription factors with extrinsic signals. (amegroups.org)
  • They behave as wild cells, so trying to control them for human therapy has been a safety challenge. (progencell.com)
  • We are specialists in different treatments such as stem cell therapy for MS in Mexico , for COPD, diabetes, stem cell therapy for rheumatoid arthritis , Alzheimer, and more. (progencell.com)
  • In addition, we summarize the recent direct reprogramming of cardiomyocytes from fibroblast cells, which provides another method for potential heart disease therapy. (biomedcentral.com)
  • Another important facet of our work is the use of CRISPR/Cas9 gene editing for many applications including the generation of reporter cell lines and the design of novel gene therapy approaches. (tum.de)
  • Mora MV, Ibán MAR, Heredia JD, Laakso RB, Cuéllar R, Arranz MG. Stem cell therapy in the management of shoulder rotator cuff disorders. (wjgnet.com)
  • However, the standardization of the manufacturing process of MSC-based cell therapy medicinal products in compliance with the requirements of the local authorities is obligatory and will allow us to obtain the necessary permits for product administration according to its intended use. (mdpi.com)
  • Improved understanding of the role that each cell component plays U-93631 towards cell mechanics may be beneficial for diagnosis and therapy of diseases [2]. (globaltechbiz.com)
  • Cell therapy using articular chondrocytes overexpressing BMP-7 or BMP-10 in a rabbit disc organ culture model. (uchicago.edu)
  • In this review, we describe the cardiac differentiation from ES cells, iPS cells, and the current progress of using iPS cell-derived cardiomyocytes for heart disease modeling and for the development of therapeutic strategies. (biomedcentral.com)
  • The neomycin-resistant gene driven by the cardiac α-myosin heavy chain promoter was stably transfected into ES cells. (biomedcentral.com)
  • Live immunofluorescence imaging of a 3D cardiac spheroid derived from a double reporter stem cell line expressing eGFP and mCherry in the endogenous loci of the cardiac transcription factors NKX2.5 and TBX5 , respectively. (tum.de)
  • Reagents and instruments for immunology, cell biology and molecular biology. (biotrend.com)
  • The most prominent example is the changes in cell biology and morphology when it evolves from a healthy to a cancerous state [1, 3]. (globaltechbiz.com)
  • Prior studies of acute phosphate restriction during the endochondral phase of fracture healing showed delayed chondrocyte differentiation was mechanistically linked to decreased bone morphogenetic protein signaling. (researchgate.net)
  • Currently, there is no consensus whether there is a single or multiple postnatal stem cell population(s) that contribute to skeletal homeostasis and postnatal bone formation. (researchgate.net)
  • A known population of cells that express Prx1 contributes to postnatal bone formation. (researchgate.net)
  • NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
  • increased the expression of myocardin, caldesmon, 22-kDa smooth muscle cell-specific protein (SM22α), and SM-myosin heavy chains in rabbit bone marrow-derived mesenchymal stem cells , as detected by reverse transcription polymerase chain reaction (PCR). (zju.edu.cn)
  • In addition, more than 47% of bone marrow-derived mesenchymal stem cells demonstrated the contractile phenotype of smooth muscle cells . (zju.edu.cn)
  • Inhibitor of DNA binding/differentiation helix-loop-helix proteins mediate bone morphogenetic protein-induced osteoblast differentiation of mesenchymal stem cells. (uchicago.edu)
  • Comparative effects of bone morphogenetic proteins and Sox9 overexpression on matrix accumulation by bovine anulus fibrosus cells: implications for anular repair. (uchicago.edu)
  • The development of the hematopoietic system has long served as model to understand how changes in gene regulatory networks drive the differentiation of the various blood cell types. (amegroups.org)
  • HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
  • Collectively, these are referred to as hematopoietic stem and progenitor cells (HSPCs). (stemcell.com)
  • There his work focused on transcriptional control of hematopoietic stem cell maturation and cell fate. (ubc.ca)
  • In addition, he identified a number of novel hematopoietic stem cell surface proteins and began analyzing their function. (ubc.ca)
  • In this regard, his lab has identified a novel family of hematopoietic cell surface proteins, called the CD34 family, and shown that these are essential for a number of developmentally important processes. (ubc.ca)
  • The volumetric analysis of cellular expression patterns provides evidence for molecular mechanisms regulating the development of pre-implantation mouse embryos. (zeiss.com)
  • This simplified approach towards understanding the essence of the mechanisms, underlying the processes determining life and death of a cell has undoubtedly provided scientists with enormous amount of knowledge. (intechopen.com)
  • Herein in this review, we aimed to address the roles delivered by MECs in breast cancer progression and decipher the molecular mechanisms regulating proper MECs' physiology, integrity, and terminal differentiation. (biomedcentral.com)
  • A switch from glycolysis to mitochondrial respiration drives these cells toward differentiation, but the mechanisms that control this switch are poorly defined. (lww.com)
  • We found that the overexpression of HIF-2α and VM were correlated with poor tumor differentiation, late clinical stage and lymph node metastasis, and a poor prognosis in pancreatic cancer. (oncotarget.com)
  • Osteogenic BMPs promote tumor growth of human osteosarcomas that harbor differentiation defects. (uchicago.edu)
  • Expression of the Arf tumor suppressor gene is controlled by Tgfbeta2 during development. (uchicago.edu)
  • Specifically, human iPS cells could be utilized to generate patient-specific lineages for a variety of translational research. (biomedcentral.com)
  • CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
  • The iPSC technology was pioneered by Shinya Yamanaka and Kazutoshi Takahashi in Kyoto, Japan, who together showed in 2006 that the introduction of four specific genes (named Myc, Oct3/4, Sox2 and Klf4), collectively known as Yamanaka factors, encoding transcription factors could convert somatic cells into pluripotent stem cells. (wikipedia.org)
  • Embryonic stem (ES) cells are naturally derived from early stage embryos and induced pluripotent stem (iPS) cells are reprogrammed from somatic cells with overexpression of four reprogramming factors, Oct4, Sox2, Klf4 and c-Myc. (biomedcentral.com)
  • Human induced pluripotent stem cells (hiPSCs) are stem cells that are derived from the somatic cells (e.g. blood or skin cells) of healthy individuals or patients. (tum.de)
  • These cells, surrounding the hair follicles, secrete signaling molecules that control the progression of the hair cycle. (jcadonline.com)
  • The cells secrete extracellular vesicles (EV) that may have an endosomal origin, or from evaginations of the plasma membrane. (frontiersin.org)
  • 2. Stem cells secrete growth factors which can help heal damage cells. (drcremers.com)
  • Finally, we shed the light on the key molecular signalling pathways that are involved in the regulation of MECs' development and functions. (biomedcentral.com)
  • Once a therapeutic target has been identified, we also use hiPSC-derived cells as a platform for testing novel therapies, as we have previously done for Duchenne muscular dystrophy (DMD). (tum.de)
  • In order to find more promising treatments to stimulate renal repair, stem cell-based technology has been proposed as a potentially therapeutic option. (biomedcentral.com)
  • So as a new therapeutic approach, stem cells are under research with a focus on reducing the burden of several kidney diseases. (biomedcentral.com)
  • Replacing dying or diseased kidney cells with new nephrons is an attractive strategy. (emjreviews.com)
  • Our findings identify a novel role for VHL in mediating nephron progenitor differentiation through metabolic regulation, and suggest that VHL is required for normal kidney development. (lww.com)
  • To study the potential role in this metabolic shift of von Hippel-Lindau (VHL), a protein component of a ubiquitin ligase complex, the authors generated nephron progenitor cell-specific VHL knockout mice. (lww.com)
  • They are 3-dimensional, complex, multicellular structures that can be derived from patient specific human induced pluripotent stem cells (hiPSCs). (nature.com)
  • The human body contains many different cells, which carry out specific functions. (progencell.com)
  • This is a special kind of stem cell, called a zygote and has the potential to grow into a human being. (progencell.com)
  • It has the ability to form any type of cell in a human being and second, it possesses the capability of producing an unlimited number of cells without losing its total potency. (progencell.com)
  • Mature human red blood cell (RBC) is perhaps among the simplest cells to model, lacking nucleus and internal cytoskeleton. (globaltechbiz.com)
  • The tumorigenicity of human embryonic stem cells. (zju.edu.cn)
  • The immune response of human the body can amplify the process, increasing the local inflammation response and giving rise to the development of interstitial pneumonia. (biomedcentral.com)
  • Mei H, González S, Nakatsu MN, Baclagon ER, Chen FV, Deng SX (2017) Human adipose-derived stem cells support the growth of limbal stem/progenitor cells. (drcremers.com)
  • Maduro, M. F. Structure and evolution of the C. elegans embryonic endomesoderm network. (nature.com)
  • Acute liver failure is a life-threatening clinical syndrome characterized by rapid development of hepatocellular necrosis leading to high mortality and resource costs. (springer.com)
  • The standard method to culture LSCs on 3T3 feeder cells that have been used in clinical study is cultivating single LSC directly on top of the growth-arrested 3T3 feeder cells [ 14 ]. (drcremers.com)
  • The results of these analyses revealed that the differentially expressed exosomal miRNAs participate in multiple biological processes, such as gene expression, synthesis of biomolecules, cell development, differentiation, and signal transduction, among others. (hindawi.com)
  • The stimulus for the change of cell fate originates from extrinsic signals which set a cascade of intracellular processes in motion that eventually terminate at the genome leading to changes in gene expression and the development of alternate gene regulatory networks. (amegroups.org)
  • This selectivity suggests that the commitment of CNS precursor cells to form glia or neuron involves the direct regulation of the single synemin gene. (biomedcentral.com)
  • As the development phase continues, a series of signals or instructions gradually limit each cell's potential. (progencell.com)
  • Out of all the stem cells, it has the lowest differentiation potential. (progencell.com)
  • 2011). During wounding of tree barks, callus can be Lu AF21934 shaped from multiple vasculature-associated cells and may generate a number of fresh ones, suggesting it offers some pluripotent potential (Stobbe et al. (cancercurehere.com)
  • Specific properties, such as proliferative and differentiation potential, are slightly different among DSCs. (bvsalud.org)
  • Because they can propagate indefinitely, as well as give rise to every other cell type in the body (such as neurons, heart, pancreatic, and liver cells), they represent a single source of cells that could be used to replace those lost to damage or disease. (wikipedia.org)
  • Pluripotent stem cells have a widespread use in the treatment of various diseases and conditions like heart disease, diabetes, burn victims and even neurological disorders like Parkinson's disease. (progencell.com)
  • Because in order to avoid renal injury, the recovery of affected cells is as significant as preventing its progression towards end stage renal disease [ 1 ]. (biomedcentral.com)
  • Introduction Cell mechanics has proved to be a widely used label-free biomarker to discern phenotypes, detect pathologies and more importantly, monitor existence or progression of a disease [1C3]. (globaltechbiz.com)
  • Secondly, they can improve our understanding of the adjustments that occur during disease progression which, in turn, might uncover reasons for corresponding alterations occurring in cell mechanics [10, 11]. (globaltechbiz.com)
  • We used histologic, immunofluorescence, RNA sequencing, and metabolic assays to characterize kidneys from these mice and controls during development and up to postnatal day 21. (lww.com)
  • Infection of iPSCs with lentiviral inserts is a highly efficient process since stem cells grow quickly, remain undifferentiated in specific cell culture conditions and can establish fully infected clones within 2-3 passages. (nature.com)
  • For the first few divisions, all the cells remain undifferentiated. (progencell.com)