• Conditioned medium from adipose derived stem cells (ADSC-CM) stimulates both collagen synthesis and migration of fibroblasts, and accelerates wound healing in vivo. (indexindex.com)
  • After ADSC-CM addition, collagen type I, type III, HAS and hyaluronic acid (HA) expressions on human dermal fibroblasts (HDFs) were evaluated. (indexindex.com)
  • Besides endothelial cells and fibroblasts, sodium also affects innate and adaptive immunometabolism, immune cell function, and influences certain microbes and microbiota-derived metabolites. (bvsalud.org)
  • iPS were generated via a platelet lysate or fetal bovine serum-based culturing protocol from matched dermal, buccal and gingival human fibroblasts, isolated from healthy donors ( n = 2) after informed consent, via episomal plasmid transfection. (biomedcentral.com)
  • Furthermore, oral fibroblasts generally required more attempts for successful iPS generation as opposed to dermal fibroblasts. (biomedcentral.com)
  • The oral fibroblasts generally proved to be more difficult to reprogram than dermal fibroblasts. (biomedcentral.com)
  • Xeno-free dermal, buccal and gingival fibroblasts can successfully generate iPS with a comparable genotype/phenotype to their xenogenic counterparts. (biomedcentral.com)
  • Moreover, proliferating cell nuclear antigen (PCNA) and CD31 showed co-localization with α-SMA, suggesting the differentiation of hBMSCs into epithelial cells and myofibroblasts/fibroblasts. (ijbs.com)
  • Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. (thermofisher.com)
  • Subsequently, the scaffold was bioconjugated with apelin-13 using the EDC-NHS linker and acellular scaffolds were recellularized using fibroblasts, endothelial cells, and smooth muscle cells. (biomedcentral.com)
  • Herein, we introduce a cardiac-mimetic cell-culture system that resembles the microenvironment in the heart and provides interactions with cardiomyocytes and electrical cues to the cultured fibroblasts for direct cardiac reprogramming. (thno.org)
  • The human neonatal dermal fibroblasts containing cardiac transcription factors were plated on the membrane and cultured with the murine cardiomyocyte in the presence of the electric stimulus. (thno.org)
  • In vitro experiments withcardiac fibroblasts and H9c2 cells confirmed the exceptional biocompatibilityof BC-Ppy composites. (ibecbarcelona.eu)
  • Applying MPTR to dermal fibroblasts from middle-aged donors, we found that cells temporarily lose and then reacquire their fibroblast identity, possibly as a result of epigenetic memory at enhancers and/or persistent expression of some fibroblast genes. (elifesciences.org)
  • In the present study, we show that let-7c miRNA is decreased in heat-denatured fibroblasts and that inhibiting let-7c expression leads to the increased proliferation and migration of dermal fibroblasts, whereas the overexpression of let-7c exerts an opposite effect. (molcells.org)
  • 6. Mogami H, Keller PW, Shi H, Word RA (2014) Effect of Thrombin on Human Amnion Mesenchymal Cells, Mouse Fetal Membranes, and Preterm Birth. (yoproteins.eu)
  • We previously demonstrated that mesenchymal stem cells (MSCs) ameliorated experimental autoimmune uveoretinitis (EAU) in rats. (nature.com)
  • Mesenchymal stem cell (MSC)-based therapy for the treatment of autoimmune diseases has demonstrated a particular promise after its successful applications in both animal models and patients. (nature.com)
  • We further discuss how injuries alter tissue environments and how this primes mononuclear phagocytes to enforce this particular environment, for example, to support host defense and pathogen clearance, to support the resolution of inflammation, to support epithelial and mesenchymal healing, and to support the resolution of fibrosis to the smallest possible scar. (hindawi.com)
  • Mesenchymal stem/stromal cells (MSCs)‐based therapy brings the reassuring capability to regenerative medicine through their self‐renewal and multilineage potency. (biomedcentral.com)
  • After that, Kaplan firstly proposes the term "mesenchymal stem cells," which are cells isolated from fully developed bone marrow (BM) that can usually differentiate into several types of mesenchymal origin cells [ 4 ]. (biomedcentral.com)
  • Mesenchymal stromal cells (MSCs) expanded in vitro have been proposed as a potential therapy for congenital or acquired skin defects in pediatrics. (biomedcentral.com)
  • The aim of this pre-clinical study was to investigate the effects of intradermal injections of MSC in experimental cutaneous wound repair comparing allogeneic and autologous adipose stem cells (ASCs) and autologous bone marrow-mesenchymal stromal cells (BM-MSCs). (biomedcentral.com)
  • Mesenchymal stromal cells were in vitro expanded from adipose and BM tissues of young female New Zealand rabbits. (biomedcentral.com)
  • Fracture Healing in Diabetes Fracture repair entails recruitment of mesenchymal stem cells after which differentiation to chondrocytes that type cartilage (see Chapter 12). (dnahelix.com)
  • While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
  • There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
  • Mariano García Arranz has the following conflict of interest: MGA is inventor on 2 patents entitled "Identification and isolation of multipotent cells from non-osteochondral mesenchymal tissue" (10157355957US) and "Use of adipose tissue-derived stromal stem cells in treating fistula" (US11/167061). (wjgnet.com)
  • 6,7 Mesenchymal stem cells (MSCs) have also been isolated from other sources, such as bone marrow, 8 the placenta, 9 muscle, 10 or blood. (jcadonline.com)
  • 4 HFSCs are located in the bulge region of the follicle and they interact with mesenchymal SCs (MSCs) located in the dermal papilla (DP). (benitonovas.com)
  • Loss of tissue is then repaired in a complex process that involves tissue-resident immune, stromal, and epithelial cells, as well as infiltrating immune cells [ 2 ]. (biomedcentral.com)
  • CD326 is expressed on the majority of epithelial cells, and is considered a pan-carcinoma antigen. (thermofisher.com)
  • Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. (thermofisher.com)
  • Recent investigations have shown that the RON receptor is expressed in various cell types including epithelial cells (23) and that MSP-mediated effects on epithelial cells are integrin-dependent (23, 24). (123dok.org)
  • Our group recently reported that CD73 on the cell membrane also contributes to the immunomodulatory capacity of MSCs 3 , suggesting that MSCs exert their immunomodulatory properties in a multifactorial manner. (nature.com)
  • Although there is no particular quantitative assay to provide MSCs identification in mixed cells population [ 8 ], the International Society for Cellular Therapy (ISCT) has provided minimum principles to determine MSCs. (biomedcentral.com)
  • However, natural MSCs in vivo survival and their biological effects on tissue recovery decrease with long-term cultivation called aging and also injected cells demonstrate poor targeted migration [ 21 ]. (biomedcentral.com)
  • MSCs were characterized for plastic adhesion, surface markers, proliferation and differentiation capacity. (biomedcentral.com)
  • When an adequate number of cells (ASCs 10 × 10 6 and BM-MSCs 3 × 10 6 , because of their low rate of proliferation) was reached, two skin wounds were surgically induced in each animal. (biomedcentral.com)
  • MSCs have been isolated from various tissues such as bone marrow (BM), umbilical cord blood, skeletal muscle and brain. (biomedcentral.com)
  • For instance ASCs show a higher proliferative capacity and retain differentiation potential for a longer period in culture compared with human BM-MSCs. (biomedcentral.com)
  • Moreover it has been demonstrated that human ASCs support hematopoiesis both in vitro and in vivo more efficiently than human BM-MSCs, on the other hand it has been suggested that BM-MSCs evoke less inflammation and thrombogenesis than ASC [ 16 ]. (biomedcentral.com)
  • The public clinical trials database http://clinicaltrials.gov shows 123 clinical trials using MSCs for a very wide range of therapeutic applications (Figure 1 ), the majority of which are in Phase I (safety studies), Phase II (proof of concept for efficacy in human patients), or a mixture of PhaseI/II studies (Figure 2 ). (biomedcentral.com)
  • This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
  • The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
  • 11 However, the greater the abundance of adipose tissue in the human body, the significantly higher the yield of MSCs is compared to other tissues. (jcadonline.com)
  • 24 - 26 ECs may provide signals for BMP receptor activation in DPCs similar to those signals that promote survival of MSCs in human embryoid bodies composed of multipotent cells. (benitonovas.com)
  • Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD. (springer.com)
  • In order to eliminate the interference from the transplanted cells themselves, fetal stem cells were used for transplantation because the proliferation and differentiation potentials of fetal stem cells are superior to the adult ones ( 8 ). (ijbs.com)
  • Therefore, we aimed to compare the efficiency of using humanized culture conditions, specifically human platelet lysate to fetal bovine serum, for iPS generation from different sources, and to evaluate their stemness. (biomedcentral.com)
  • A classification that sufficiently covers their phenotypic heterogeneity and plasticity during homeostasis and disease does not yet exist because cell culture-based phenotypes often do not match those found in vivo . (hindawi.com)
  • In this Chapter, we dissect the proof-of-principle studies that demonstrated that cellular reprogramming to pluripotency can be induced in vivo, in spite of unfavorable pro-differentiation signals present within the tissues. (springer.com)
  • The links of in vivo reprogramming to pluripotency with tumorigenesis and teratoma formation, and the cross-talk with cellular senesce and tissue injury are also discussed. (springer.com)
  • Her research interests include the preparation and design of materials and scaffolds for in vitro and in vivo fundamental studies, and a further focus is the provision of useful tools to assess mechanisms that govern cell behavior in regenerative medicine. (ibecbarcelona.eu)
  • Pre-clinical studies are however necessary to validate the best skin regeneration technique, which could be used in pediatric surgical translational research. (biomedcentral.com)
  • Recent advances in stem cell therapy for tissue engineering approaches have provided promising data on wound repair and tissue regeneration. (biomedcentral.com)
  • She was awarded with a postdoc fellow from the Ministry of Science at the Technical University of Catalonia in Biomaterials for bone tissue regeneration and since 2010 has been professor at this university. (ibecbarcelona.eu)
  • The Sonic hedgehog (SHH) signaling pathway is essential for embryonic development and tissue regeneration. (regenmedres-journal.org)
  • The advance in understanding the SHH signaling pathway will give us more clues to the mechanisms of tissue repair and regeneration, as well as the development of new treatment for diseases related to dysregulation of SHH signaling pathway. (regenmedres-journal.org)
  • 11 , 12 With the emergence of stem cells (SCs), many mechanisms that lead to tissue regeneration have been discovered. (benitonovas.com)
  • Embryonic stem cells (ESC) can be expanded indefinitely without undergoing replicative senescence or aging due to their high telomerase expression. (biomedcentral.com)
  • An immunologically identical cardiac tissue can be produced by direct reprogramming from the patients' tissue without the potential risk of tumorigenesis that occurs with induced pluripotent stem cells [ 2 ] and the ethical issue using embryonic stem cells. (thno.org)
  • the EST, which assesses the effects of compounds on the differentiation of mouse embryonic stem cells into contracting cardiomyocytes, can be used to rank the potency of chemicals within a series of alkoxyacetic acid metabolites formed from The implementation of the European REACH (Registration, glycol ethers. (cdc.gov)
  • 10. Dumevska B, Chami O, McKernan R, Divya Goel Uli Schmidt (2016) Derivation of Genea052 human embryonic stem cell line. (yoproteins.eu)
  • Stem Cell Research. (yoproteins.eu)
  • Stem cell therapy has been the subject of many studies for its potential to cure many disorders. (biomedcentral.com)
  • Stem cell transplantation is a promising strategy for delayed wound healing caused by chemotherapy. (ijbs.com)
  • Nevertheless, myelosuppression can be reversed by autologous stem cell transplantation (SCT), even without additional toxicity ( 4 , 5 ). (ijbs.com)
  • The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
  • There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
  • The stem cell field witnessed a genuine breakthrough when a combination of solely four transcription factors ( Oct3 / 4 , Sox2 , Klf4 and c-Myc, OSKM ) proved enough to revert, in vitro, the differentiated status of a variety of cell types back to pluripotency, giving rise to so-called induced pluripotent stem (iPS) cells. (springer.com)
  • Embryonic stem cell lines derived from human blastocysts. (springer.com)
  • Evans MD, Kelley J. US attitudes toward human embryonic stem cell research. (springer.com)
  • Mora MV, Ibán MAR, Heredia JD, Laakso RB, Cuéllar R, Arranz MG. Stem cell therapy in the management of shoulder rotator cuff disorders. (wjgnet.com)
  • In this review, we analyze the therapeutic agents that have been used for AGA and emphasize the potential of new therapies based on advances in stem cell technologies and regenerative medicine. (benitonovas.com)
  • As greater understanding of the growth factors involved in wound healing emerges, future patient care may include scarless wound healing and transplant of tissues engineered from stem cell progenitors. (medscape.com)
  • In vitro toxicity data of these metabolites derived in the development of validated and accepted in vitro and in silico embryonic stem cell test were used as input in the PBK model to extrapolate in vitro concentration-response curves to predicted approaches is urgently needed. (cdc.gov)
  • This approach could therefore micromass test, and the embryonic stem cell test (EST) provide a means to reduce the need for animal testing in human risk assessment practices. (cdc.gov)
  • physiologically based animals because a mouse embryonic stem cell line is used. (cdc.gov)
  • They also can inspire angiogenesis by direct differentiation, cell-to-cell interaction, or paracrine effects. (biomedcentral.com)
  • In addition to activation of fibrin, thrombin activated by the coagulation cascades facilitates migration of inflammatory cells to the site of injury by increasing vascular permeability. (medscape.com)
  • The in vitro effects of MSC-Exo on immune cell migration and responder T cell proliferation were examined by chemotactic assays and lymphocyte proliferation assays, respectively. (nature.com)
  • These results suggest that MSC-Exo effectively ameliorate EAU by inhibiting the migration of inflammatory cells, indicating a potential novel therapy of MSC-Exo for uveitis. (nature.com)
  • Also, following transplantation and migration to target tissue, they encounter a harsh milieu accompanied by death signals because of the lack of proper tensegrity structure between the cells and matrix. (biomedcentral.com)
  • Treatment for this condition remains difficult and largely ineffective, because the growth of cells in the wound is inhibited ( 2 ) and chemotherapy usually leads to myelosuppression, the latter of which further compromises the migration of multipotent cells from the bone marrow ( 3 ). (ijbs.com)
  • Integrin-based effects on growth factor re-ceptors include enhancement of cell migration (11, 12), survival (13), and proliferation (14 -17). (123dok.org)
  • The timeline for cell migration in a normal wound healing process is predictable. (medscape.com)
  • These multipotent cells with innate self-renewal capacity can be in vitro expanded without losing their differentiation potential. (biomedcentral.com)
  • As summarized below and detailed in Chapter 3, health effects that have been associated with exposure to PCBs in humans and/or animals include liver, thyroid, dermal and ocular changes, immunological alterations, neurodevelop- mental changes, reduced birth weight, reproductive toxicity, and cancer. (cdc.gov)
  • Animal studies have shown that PCBs induce effects in monkeys at lower doses than in other species, and that immunological, dermal/ocular, and neurobehavioral changes are particularly sensitive indicators of toxicity in monkeys exposed either as adults, or during pre- or postnatal periods. (cdc.gov)
  • 43 Therefore, cell sheets with enhanced thickness and mechanical properties obtained through appropriate cyclic mechanical stimulation possess great potential for bone tissue engineering applications. (researchgate.net)
  • After implantation into the critical-sized calvarial defects of mice, the mechanically conditioned cell sheets significantly promoted new bone formation. (researchgate.net)
  • Findings from this study reveal that thermo-responsive elastomer, together with mechanical conditioning, can potentially be applied to prepare high-quality cell sheets for bone tissue engineering. (researchgate.net)
  • Hu G, Liu P, Feng J, Jin Y. Transplantation with Bone Marrow Stromal Cells Promotes Wound Healing Under Chemotherapy through Altering Phenotypes. (ijbs.com)
  • Herein we characterized human fetal bone marrow stromal cells (hBMSCs) during wound healing in mice treated with cyclophosphamide (CTX). (ijbs.com)
  • A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
  • Despite improved surgical techniques, the tendon-to-bone healing rate is unsatisfactory due to difficulties in restoring the delicate transitional tissue between bone and tendon. (wjgnet.com)
  • The epidermis is derived primarily from surface ectoderm but is colonized by pigment-containing melanocytes of neural crest origin, antigen-processing Langerhans cells of bone marrow origin, and pressure-sensing Merkel cells of neural crest origin. (medscape.com)
  • Transplantation of living nuclei from blastula cells into enucleated frogs' eggs. (springer.com)
  • Continued development of new biotechnologies also will allow farm animals to serve as sources of both biopharmaceuticals for human medicine and organs for transplantation. (nationalacademies.org)
  • Dulbecco's Phosphate Buffered Saline (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. (sigmaaldrich.com)
  • DPBS buffer is used to irrigate, wash, and dilute mammalian cells while maintaining osmotic balance. (sigmaaldrich.com)
  • The mammalian skin consists of three main layers: the epidermis, the dermis, and the subcutaneous tissue, including the fascia (Fig. 1 ). (biomedcentral.com)
  • Viable offspring derived from fetal and adult mammalian cells. (springer.com)
  • It has been found that some of the SVF cell types possess regenerative and anti-inflammatory potentials in damaged tissues due to their ability to secrete growth factors and anti-inflammatory molecules. (jcadonline.com)
  • After a short period, capillary vasodilatation occurs secondary to local histamine release, and the cells of inflammation are able to migrate to the wound bed. (medscape.com)
  • A more tissue-centric view of these processes, claiming that the tissues define phenotype and function of resident and infiltrating immune cells to meet tissues needs during homeostasis and disease, seems provocative [ 4 , 5 ]. (hindawi.com)
  • Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. (thermofisher.com)
  • Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)
  • Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
  • In HL-60 leukaemia cells, di-GA activated caspase 3 and dose-dependently induced apoptosis. (nature.com)
  • These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
  • Although most cells have vitamin D receptors, several key genes that encode proteins and peptides are modulated in part by vitamin D and thus affect musculoskeletal functions, mitochondrial respiration, cell growth, proliferation, differentiation, and apoptosis [13]. (vitamindwiki.com)
  • In turn, the plant produces GA, which seems to combat the improper growth signals and re-establishes cell-cycle control. (nature.com)
  • In the heart, cardiomyocytes interact with neighboring cells through direct cell-cell interactions, various secreted cytokines, and electric signals (Figure 1 A) [ 24 ]. (thno.org)
  • 15 , 16 Some of these signals stimulate differentiation of SCs to keratinocytes which are important for HF growth. (benitonovas.com)
  • 17 Other signals can stimulate dermal papilla cells (DPCs) that promote SC proliferation in the HF. (benitonovas.com)
  • 19 - 21 Other signals, such as those from endothelial cells (ECs) located at the DP, are also essential for HF maintenance. (benitonovas.com)
  • Wound healing is a complex physiological process necessitating the coordinated action of various cell types, signals and microRNAs (miRNAs). (molcells.org)
  • Stem cells and regenerative medicine - future perspectives. (springer.com)
  • Stem cells in regenerative medicine: introduction. (springer.com)
  • DIBP can be absorbed via oral ingestion and dermal exposure. (wikipedia.org)
  • Due to the increase in human exposure to phthalates, in 1999 the European Union restricted the use of some of them in children's toys. (wikipedia.org)
  • Human PCB exposure has also been attributed to inhalation of indoor air, especially at locations that still use electrical equipment containing PCBs. (cdc.gov)
  • An important issue related to evaluating health effects of PCBs in humans is exposure assessment. (cdc.gov)
  • Exposure to PCBs has been assessed by measuring PCBs in blood, breast milk, and adipose tissue. (cdc.gov)
  • The preponderance of the biomedical data from human and laboratory mammal studies provide strong evidence of the toxic potential of exposure to PCBs. (cdc.gov)
  • The human studies of the Yusho and Yu-Cheng poisoning incidents, contaminated fish consumption, and general populations are complicated by the mixture nature of PCB exposure and possible interactions between the congeneric components and other chemicals (see Chapter 3 for additional information). (cdc.gov)
  • Following in vitro exposure to CTX, hBMSCs showed decreased cell growth in a dose- and time-dependent manner, accompanied by increased expressions of collagen-I/III, and CD31. (ijbs.com)
  • Exposure to a potential fetal health hazard can be estimated through ecological assessment (e.g., testing the community water supply), questionnaires (e.g., classifying residents accord- ing to whether they drink bottled or tap water), or biological markers (e.g., testing for exposures to chemicals or solvents found in tap water). (cdc.gov)
  • Specific Treg then provide active tolerance to the harmless food proteins at all mucosal surfaces, either via cell contact or via their secretion of immunosuppressive factors that both prevent the induction of adaptive T helper (Th) lymphocytes in response to antigenic re-exposure ( 7 ). (frontiersin.org)
  • Public health officials and others concerned with appropriate actions to take at hazardous waste sites may want information on levels of exposure associated with more subtle effects in humans or animals (LOAEL) or exposure levels below which no adverse effects (NOAELs) have been observed. (cdc.gov)
  • Estimates of exposure levels posing minimal risk to humans (Minimal Risk Levels or MRLs) have been made for di-n-butyl phthalate. (cdc.gov)
  • An MRL is defined as an estimate of daily human exposure to a substance that is likely to be without an appreciable risk of adverse effects (noncarcinogenic) over a specified duration of exposure. (cdc.gov)
  • Appropriate methodology does not exist to develop MRLs for dermal exposure. (cdc.gov)
  • As these kinds of health effects data become available and methods to assess levels of significant human exposure improve, these MRLs will be revised. (cdc.gov)
  • Nevertheless, over exposure to sunlight, does not cause hypervitaminosis D but can cause damage to skin cells. (vitamindwiki.com)
  • Most of the vitamin D requirement in humans can be and, is supposed to be generated following exposure to sunlight. (vitamindwiki.com)
  • It serves as a protective barrier that prevents internal tissues from exposure to trauma, ultraviolet (UV) radiation, temperature extremes, toxins, and bacteria. (medscape.com)
  • The Hh gene family encodes a secreted protein that plays a critical regulatory role in the process of embryogenesis and environmental stabilization in adult tissues of invertebrates and vertebrates [ 44 ]. (regenmedres-journal.org)
  • Advances in tissue engineering technology have led to the production of novel human skin equivalents and organoids that reproduce cell-cell interactions with tissue-scale tensional homeostasis, and enable us to evaluate skin tissue morphology, functionality, drug response and wound healing. (mdpi.com)
  • Wound healing is a natural restorative response to tissue injury. (medscape.com)
  • Meanwhile, granulation tissue forms and the wound begins to contract. (medscape.com)
  • Vasodilation is an important means by which the wound can be exposed to increased blood flow, accompanied by the necessary inflammatory cells and factors that fight infection and débride the wound of devitalized tissue. (medscape.com)
  • Alterations in pH (secondary to tissue and bacterial degradation), swelling, and tissue hypoxemia at the injury site contribute to the sensation of wound pain. (medscape.com)
  • Neutrophils are the predominant cell type for the first 48 hours after injury but do not appear essential to the wound-healing process. (medscape.com)
  • Macrophages are essential to wound healing and perhaps are the most important cells in the early phase of wound healing. (medscape.com)
  • Mononuclear phagocytes contributions to tissue pathologies relate to their central roles in orchestrating all stages of host defense and wound healing, which often become maladaptive processes, especially in sterile and/or diffuse tissue injuries. (hindawi.com)
  • These cells were implanted into the surgical wound dermis in the suprapubic region before skin synthesis. (rbcp.org.br)
  • The subsequent wound healing processes aim to restore dermal and epidermal integrity and functionality. (biomedcentral.com)
  • In this context, regulatory T cells (Tregs) hold a key role in balancing immune homeostasis and mediating cutaneous wound healing. (biomedcentral.com)
  • The maintenance of tissue homeostasis and proper wound healing is, therefore, a critical process that can be disturbed by a plethora of external and internal factors. (biomedcentral.com)
  • Specifically, the timely orchestration of pro- and anti-inflammatory responses, based on the activation and inhibition of various cell types, such as tissue-resident cells and different types of immune cells, can significantly impact wound repair [ 7 ]. (biomedcentral.com)
  • While researchers agree on the abundance of Tregs in distinct dermal layers, the exact involvement of Tregs in cutaneous wound healing remains to be elucidated [ 18 ]. (biomedcentral.com)
  • Understanding how the body repairs damaged tissue and what factors influence the wound healing process helps the surgeon ensure an acceptable outcome from surgery. (medscape.com)
  • Wound healing in any tissue follows a predictable sequence of events. (medscape.com)
  • Although seemingly basic in concept, advances in molecular science have allowed modern medicine to gain a true appreciation of the complex interplay between the cells involved in the phases of wound healing. (medscape.com)
  • After injury to tissue occurs, the cell membranes, damaged from the wound formation, release thromboxane A2 and prostaglandin 2-alpha, potent vasoconstrictors. (medscape.com)
  • Cells involved in wound healing. (medscape.com)
  • The cells appearing in a wound are depicted in sequence from left to right, and the color bars represent the range of days each cell type is in the wound. (medscape.com)
  • An immune-centric view assumes that a particular priming of phagocytes then causes a particular type of pathology in target tissues, conceptually similar to antigen-specific T-cell priming. (hindawi.com)
  • It functions by slowing or blocking cell growth and compromising the immune system's response. (ijbs.com)
  • To this end, multiple tissue-resident cells and recruited immune cells cooperate to efficiently repair the injured tissue. (biomedcentral.com)
  • This function of the cutaneous system is mediated by a myriad of highly specialized immune cells. (biomedcentral.com)
  • In interaction with other non-immune cells, such immune cell subsets ensure the functionality and integrity of the skin layers [ 1 ]. (biomedcentral.com)
  • To this end and within their broad functionality and operationality, Tregs are able to adopt unique features of skin cells and contribute to the skin's role as an immune barrier. (biomedcentral.com)
  • Biologic factors that include signaling from stem cells, dermal papilla cells, and platelet-rich plasma are some of the current therapeutic agents being studied for hair restoration with milder side effects. (benitonovas.com)
  • Intradermal adipocyte progenitor cells are activated toward the end of the telogen phase to proliferate and differentiate into mature adipocytes. (jcadonline.com)
  • 26,27 Dermal adipocytes are organized in small clusters under the reticular dermis and interact with the hair follicular cells regulating the hair cycle. (jcadonline.com)
  • showed that the differentiation assay of benchmark dose. (cdc.gov)
  • Nano-thin and nano-porous membranes and heart like electric stimulus were used in the cardiac-mimetic cell-culture system. (thno.org)
  • Recently, the production and secretion of growth factors has been identified as an essential function of adipose-derived stem cells (ADSCs). (indexindex.com)
  • The main property that sets ESC apart from other cells is their pluripotent nature, meaning they can give rise/differentiate to cells of the three primary germ layers (mesoderm, endoderm and ectoderm) [ 13 ]. (biomedcentral.com)
  • During the fourth week of embryologic development, the single cell thick ectoderm and underlying mesoderm begin to proliferate and differentiate. (medscape.com)
  • Exposed subendothelium, including collagen and tissue factor, activates platelet aggregation and leads to clot formation (primary plug). (medscape.com)
  • Chemokines released by platelet activation attract inflammatory cells to the area, leading to the next phase in the healing process. (medscape.com)
  • Collagen type I was used in cell substrates to various stiffness. (yoproteins.eu)
  • HeLa cell are detached by non-enzymatic dissociation buffer and reseeded onto 96-well polystyrene plate coated with 40 ug/ml collagen I (CC050, Millipore) for 30 mins. (yoproteins.eu)
  • 7. Duruksu G and Aciksari A (2018) Guiding the Differentiation Direction of Pancreatic Islet-Derived Stem Cells by Glycated Collagen. (yoproteins.eu)
  • Y-27632 eye drops (or vehicle only) were administered to eyes after a superficial keratectomy, and the tissue was examined by immunohistochemistry for α-SMA, collagen types I, II, and III, and keratan sulfate. (molvis.org)
  • Interestingly, bundles of aligned and uniformly spaced collagen fibrils were more prevalent in keratocytes in Y-27632-treated corneas, which is reminiscent of fibripositor-like structures that have been proposed as a mechanism of matrix deposition in embryonic connective tissues. (molvis.org)
  • 48,49 In addition, cyclic mechanical strain enhances the function and development of engineered tissues by improving the production of collagen and elastin. (researchgate.net)
  • These epidermal and connective tissue layers protect against mechanical and chemical harm and repel pathogenic invaders [ 19 ]. (biomedcentral.com)
  • Adipose tissue is a loose connective tissue composed of cells supported by an intracellular matrix as well as by vascular, lymphatic, and neural networks. (jcadonline.com)
  • In this review, we discuss the potential influence of stromal vascular fraction from adipose tissue in the management of alopecia as well as its involvement in preclinical and clinical trials. (jcadonline.com)
  • however, a variety of other cell types are found as well, collectively labeled as stromal vascular fraction (SVF). (jcadonline.com)
  • Thus, the secretion of gall wasps stimulates plant cell growth and overrules homeostasis of the affected leaf area - this is similar to tumour outgrowth. (nature.com)
  • A tissue-centric view assumes that changing tissue microenvironments shape the phenotypes of their resident and infiltrating mononuclear phagocytes to fulfill the tissue's need to maintain or regain homeostasis. (hindawi.com)
  • Thus, organ- and disease phase-specific microenvironments determine macrophage and dendritic cell heterogeneity in a temporal and spatial manner, which assures their support to maintain and regain homeostasis in whatever condition. (hindawi.com)
  • We examine tissue needs to maintain homeostasis and how to regain homeostasis upon tissue injury. (hindawi.com)
  • These cells provide several important physiological functions during homeostasis (Figure 1 ). (hindawi.com)
  • Diseases associated with defects in adipocyte homeostasis, such as lipodystrophy and focal dermal hypoplasia, lead to alopecia. (jcadonline.com)
  • CD326 mediates calcium-independent, homophilic, cell-cell adhesion and may function as a growth factor receptor. (thermofisher.com)
  • Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. (thermofisher.com)
  • In conclusion, our results provide a platform in which xenograft vessels are decellularized properly in a short time, and the recellularization process is significantly improved after the bioconjugation of the acellular scaffold with apelin-13 in terms of cell adhesion and viability within the scaffold. (biomedcentral.com)
  • The pathways involved in cellular differentiation or transformation are Smad, Rho proteins, and PI3-kinase. (molvis.org)
  • These omics techniques, previously mostly used on bulk tissue and individually, are increasingly available at a single cellular level and can be combined with each other. (bvsalud.org)
  • These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
  • The effect of PNIPAAm grafting yields on cell behaviours was investigated to optimize surfaces suitable for cell sheet culturing and harvesting. (researchgate.net)
  • Tissue engineering of native vessels is an alternative approach for patients with vascular disease who lack sufficient saphenous vein or other suitable conduits for autologous vascular graft. (biomedcentral.com)
  • The immunohistochemical (IHC) staining of CD31, α-SMA, and vimentin markers suggested placement of seeded cells in the suitable sites and considerable elevation of cell attachment within the scaffolds bioconjugated with apelin-13 compared to the non-bioconjugated, and decellularized groups. (biomedcentral.com)
  • For the prevention of hypokalaemia, doses of potassium chloride 2вЂ"4 g (approximately 25вЂ"50 mmol) daily by mouth are suitable in most sufferers consuming a standard food plan arrhythmia research technology stock [url=https://pmsttc.edu.pk/buy/Norvasc/]discount norvasc 2.5 mg with amex[/url]. (ehd.org)
  • 27 Together, dermal blood vessels and DPCs orchestrate a suitable microenvironment for the growth and survival of HFSCs. (benitonovas.com)
  • The adult stem cells used were autologous and were extracted from infraumbilical adipose tissue prior to abdominoplasty. (rbcp.org.br)
  • Mononuclear phagocytes are a group of phenotypic distinct members, often referred to as either macrophages or dendritic cells (DC), that derive from myeloid precursors and that contribute to the functions of peripheral tissues [ 1 ]. (hindawi.com)
  • Biochemicalanalyses revealed that H9c2 cells showed different cardiomyocyte phenotypesand distinct levels of maturity depending on the amount of Ppy inthe substrate used. (ibecbarcelona.eu)
  • We found that MSC-Exo greatly reduced the intensity of ongoing EAU as their parent cells by reducing the infiltration of T cell subsets, and other inflammatory cells, in the eyes. (nature.com)
  • Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3^+ cells. (thermofisher.com)
  • Placental abnormalities cause impaired fetal growth and poor pregnancy outcome (e.g. preeclampsia [PE]) with long-lasting consequences for the mother and offspring. (bvsalud.org)
  • Metaanalyses have proven a lower threat for vertebral fracture compared with hip fracture, which can be partly as a outcome of underreporting of vertebral fractures in lots of research. (dnahelix.com)
  • It is essential for the physician to have knowledge of the healing process in order to handle the tissues correctly and obtain an optimal outcome. (rbcp.org.br)
  • More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)
  • Extracellular matrix scaffolds derived from porcine small intestinal submucosa (SIS-ECM) have been shown to promote the formation of site-specific tissue in a number of preclinical animal studies. (researchgate.net)
  • Degenerated human intervertebral discs contain autoantibodies against extracellular matrix proteins. (yoproteins.eu)
  • However, efficient harvest and handling of cell sheets remain challenging, including insufficient extracellular matrix content and poor mechanical strength. (researchgate.net)
  • Mechanical loading has been widely used to enhance extracellular matrix production in a variety of cell types. (researchgate.net)
  • We found that the extracellular matrix content and thickness of cell sheet were markedly elevated upon appropriate mechanical conditioning. (researchgate.net)
  • Our results proposed 16-h perfusion of 0.25% sodium dodecyl sulfate (SDS) + 0.5% Triton X-100 combination to the vessel as an optimal decellularization protocol in terms of cell elimination as well as extracellular matrix preservation. (biomedcentral.com)
  • 8 nm).Ppy NPs effectively augment the conductivity, surface roughness, andthickness of BC composites despite reducing scaffolds' transparency.BC-Ppy composites were flexible (up to 10 mM Ppy), maintained theirintricate 3D extracellular matrix-like mesh structure in all Ppy concentrationstested, and displayed electrical conductivities in the range of nativecardiac tissue. (ibecbarcelona.eu)
  • It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
  • Despite the cited progress in this area of research, the molecular mechanisms underlying growth factor receptor activation by integrins remain to be defined. (123dok.org)
  • However, most of the mechanisms exerted by these factors in hair restoration are still being researched. (benitonovas.com)
  • Comparing and contrasting the molecular mechanisms controlling BAT growth and development in mice and humans will increase our understanding or how human BAT is developed and may identify potential therapeutic targets to increase EE. (biomedcentral.com)
  • MicroRNAs are molecular mechanisms involved in mouse BAT development however, little is known about the miRNA profile in human BAT. (biomedcentral.com)
  • Understanding the mechanisms controling BAT differentiation may identify potential therapeutic targets to enhance BAT-controlled metabolism and to increase EE. (biomedcentral.com)
  • Feature papers represent the most advanced research with significant potential for high impact in the field. (mdpi.com)
  • Introduction The research of Hatch (1) has greatly increased our understand- ing of the use and potential limitations of biological markers for adverse reproductive effects. (cdc.gov)
  • Meanwhile, the cross-reactivity of CD8+ and CD4+ T cells response to Dengue and Zika viruses provide important clues for further development of potential treatments. (frontiersin.org)
  • From this sub-set bioinformatics analysis suggested a potential role of miR-20a in the control of cell fate and this warrants further investigation. (biomedcentral.com)
  • MiRNA screenings and bioinformatics analyses have identified potential gene targets and biological functions controlled by certain miRNAs in tissues and diseases. (biomedcentral.com)
  • Adipose cells organized in small clusters under the reticular dermis closely interact with hair follicular cells and regulate the hair cycle. (jcadonline.com)
  • Denatured dermis caused by deep skin burns is strongly associated with functional impairment, cell metabolism disorders and pathologically morphological changes. (molcells.org)
  • Induced pluripotent stem cells (iPS) can be generated from various somatic cells and can subsequently be differentiated to multiple cell types of the body. (biomedcentral.com)
  • At the same time, the first three trials using cells derived from pluripotent cells have begun. (biomedcentral.com)
  • Martin GR. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. (springer.com)
  • Pluripotent teratocarcinoma-thymus somatic cell hybrids. (springer.com)
  • Human placenta. (yoproteins.eu)
  • Galectin-1 (gal-1), a highly expressed glycan-binding protein at the maternal-fetal interface, orchestrates the maternal adaptation to pregnancy and placenta development. (bvsalud.org)
  • Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)
  • Most tissues harbor resident mononuclear phagocytes, that is, dendritic cells and macrophages. (hindawi.com)
  • In this paper we apply the tissue-centric perspective to discuss the role of resident and infiltrating macrophages and dendritic cells in different organs. (hindawi.com)
  • The establishment of oral tolerance is dependent on the controlled uptake of food proteins through the intestinal epithelial barrier and its delivery to local dendritic cells (DC) that matured in the intestinal pro-tolerogenic environment. (frontiersin.org)