Differentiation of human cellular differentiation of embryonic. Medical search. Frequent questions

The findings reveal the widespread impact of both nicotine and non-nicotine components of tobacco smoke on early embryonic development, according to Bernstein. (ucsf.edu)
The basis of in vitro differentiations are cellular and molecular processes occurring during early embryonic development. (helmholtz-munich.de)
Homeobox proteins direct the formation of body structures during early embryonic development. (medlineplus.gov)

It thoroughly investigated the unique ability of mouse embryonic stem cells to develop into cells of any organ ("pluripotency"), created new tools for functional genomic studies and thus provided key knowledge to understanding the commitment of cells to differentiate into cells of a particular germ layer. (europa.eu)
First using mouse embryonic stem cells (mESCs) then using human induced pluripotent stem (iPSCs) cells Dr. Burtscher and his team generated numerous fluorescent reporter lines. (helmholtz-munich.de)

Gene therapy and monoclonal antibodies didn't arrive with even a fraction of the hype surrounding embryonic stem cell research. (latimes.com)
As a result, induced pluripotent stem cells do not offer an easy solution to the difficult ethical questions surrounding embryonic stem cell research," he says. (sciencedaily.com)

Rab11 proteins, members of the Rab GTPase family, are known to regulate vesicular recycling during embryonic development. (researchsquare.com)

At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo . (nottingham.ac.uk)
He, then, has translated some of this knowledge in the stem cell research field contributing to develop protocols and methods for generating sub-type specific telencephalic neurons through in vitro differentiation of embryonic and neural stem cells. (michaeljfox.org)
This discovery allows for the straightforward production of a homogenous source of human functional dopaminegic neurons amenable for a cellular replacement therapy in Parkinson's disease and its in vitro modeling. (michaeljfox.org)
The need to control differentiation of embryonic stem cells in vitro presents another set of challenges. (ddw-online.com)
WDR5, also known as BIG-3, is expressed in the developing growth plate, accelerates chondrocyte and osteoblast differentiation in vitro, and regulates osteoblast differentiation during embryonic bone development. (thermofisher.com)
These findings reveal a set of cellular changes that may represent the earliest markers of in vitro hESC transition to an epithelial phenotype, before the induction of gene expression networks that guide hESC differentiation. (northwestern.edu)
These findings we translate in vitro into the culture dish using induced pluripotent human stem cells (hiPSC). (helmholtz-munich.de)
These old cells were reprogrammed in vitro to induced pluripotent stem cells (iPSC) and to rejuvenated and human embryonic stem cells (hESC): cells of all types can again be differentiated after this genuine "rejuvenation" therapy. (eurekalert.org)

It is important for osteogenic and chondrogenic differentiation of mesenchymal stem cells, and mutations in ROR2 are associated with the skeletal disorders brachydactyly type B and Robinow syndrome. (rndsystems.com)

Developmentally regulated EZH2 levels are one of the factors that can determine the higher order chromatin structure and expression pattern of the INK4b-ARF-INK4a locus, coupling human progenitor cell differentiation to proliferation control. (biomedcentral.com)
Development and homeostasis require the coordinate regulation of cell proliferation and differentiation. (biomedcentral.com)
Rap 1 is a member of a group of proteins involved in the regulation of cell proliferation and differentiation. (medscape.com)
Researchers first multiplied skin cells (fibroblasts) from a 74 year-old donor to obtain the senescence characterized by the end of cellular proliferation. (eurekalert.org)
Specifically, the protein controls the activity of genes that regulate cell growth and division (proliferation), cell maturation and specialization (differentiation), cell movement (migration), and cell survival. (medlineplus.gov)

The FUNGENES project aimed to achieve a detailed basic understanding of stem cell self-renewal and differentiation. (europa.eu)
The self-renewal and differentiation capacities of human pluripotent stem cells (hPSCs) make them a promising source of material for cell transplantation therapy, drug development, and studies of cellular differentiation and development. (ca.gov)
Promotes self-renewal and maintains pluripotency of human embryonic stem cells and mouse Epi-stem cells when used in combination with CHIR99021 (Kim et al. (stemcell.com)
In particular, they recovered their capacity for self-renewal and their former differentiation potential, and do not preserve any traces of previous aging. (eurekalert.org)
Furthermore, pathways controlling the self-renewal capacity of normal tissue SCs are frequently deregulated in human cancer. (medscape.com)

My research interests focus mainly on stem cells (embryonic, 'adult' and fetal origins) and their applications in tissue engineering, particularly the osteoblast and bone tissue. (nottingham.ac.uk)
Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells Biophysical Journal. (nottingham.ac.uk)
Similarly, when the fertilized egg divides from two cells into four cells, each of these four cells has the potential to individually form a human fetus. (jcpa.org)
Most researchers obtain embryonic stem cells from the inner mass of a blastocyst, an embryonic stage when a fertilized egg has divided into 128 cells. (jcpa.org)
The predominant bioethical concern arising from this technology is that the blastocyt-stage embryo must be destroyed in the process of isolating and separating the embryonic stem cells from the inner mass region of the pre-embryo. (jcpa.org)
The destruction of the pre-embryo has been the critical issue in the U.S. behind imposing limits on federal government-sponsored research in embryonic stem cells. (jcpa.org)
This policy is similar to that of other countries, including Israel, where scientists are funded by Government to study embryonic stem cells despite the aforementioned bioethical issue. (jcpa.org)
Contrary to popular belief, stem cells are present in the human body throughout life and are found in many adult organs. (jcpa.org)
A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
For example, RAD18 plays a vital role in regulating the DNA stability of embryonic stem cells and cellular homeostasis in highly prolific cells [ 6 ]. (nature.com)
Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. (ecampus.com)
6 Preparation of Mouse Embryonic Fibroblasts for Culture of Human Embryonic Stem Cells ( Meng-Jiao Shi, Maria Borowski, and Kimberly Stencel ). (ecampus.com)
8 Thawing, Seeding, and Changing the Medium of Human Embryonic Stem Cells ( Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski ). (ecampus.com)
9 Passaging of Human Embryonic Stem Cells on Inactivated Mouse Embryonic Fibroblast Plates ( Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski ). (ecampus.com)
10 Harvesting Human Embryonic Stem Cells for Cryopreservation ( Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski ). (ecampus.com)
13 Characterization of Human Embryonic Stem Cells by Immunofluorescence Microscopy ( Shirwin M. Pockwinse and Prachi N. Ghule ). (ecampus.com)
α-Ketoglutarate Accelerates the Initial Differentiation of Primed Human Pluripotent Stem Cells. (neurotree.org)
Lipchina I , Elkabetz Y , Hafner M , Sheridan R, Mihailovic A, Tuschl T , Sander C, Studer L , Betel D. Genome-wide identification of microRNA targets in human ES cells reveals a role for miR-302 in modulating BMP response. (neurotree.org)
Kim H, Lee G , Ganat Y , Papapetrou EP , Lipchina I , Socci ND, Sadelain M , Studer L . miR-371-3 expression predicts neural differentiation propensity in human pluripotent stem cells. (neurotree.org)
The Massachusetts-based Minerva Biotechnologies announced on 10/4 the discovery that the protein MUC1* functions as a growth receptor on human embryonic stem cells that can stimulate cellular differentiation, produce all the different cell types of an organism without producing a complete organism, and prevent spontaneous differentiation. (independent.com)
In particular, FUNGENES identified genes controlling the development of pluripotent embryonic stem (ES) cells into heart cells (cardiomyocytes), nerve cells (neurons), smooth muscle cells, vascular endothelial cells, fat cells (adipocytes), liver cells (hepatocytes) and insulin-producing cells of the pancreas. (europa.eu)
FUNGENES identified the gene subsets that are active in self-renewing cells, during the exit from pluripotency and at specific stages of mouse ES cell differentiation. (europa.eu)
Understanding the genetic pathways underlying differentiation of ES cells to somatic cells would contribute to future therapeutic strategies for degenerative diseases such as heart disease, diabetes and Parkinson's. (europa.eu)
Increased risk of genetic and epigenetic instability in human embryonic stem cells associated with specific culture conditions. (ca.gov)
We discovered that long term culture of human ESCs and iPSCs often results in selection for cells in which the TP53 gene is deleted. (ca.gov)
Efforts to produce insulin-secreting replacement cells to treat diabetes require reliable tools to assess islet cellular identity. (unige.ch)
Here, we conduct a thorough single-cell transcriptomics meta-analysis to identify robustly expressed markers used to build genesets describing the identity of human α-, β-, γ- and δ-cells. (unige.ch)
We show their efficacy to outline cell identity changes and unravel some of their underlying genetic mechanisms, whether during embryonic pancreas development or in experimental setups aiming at developing glucose-responsive insulin-secreting cells, such as pluripotent stem-cell differentiation or in adult islet cell reprogramming protocols. (unige.ch)
Critical to the hypothesis is the experimental fact that human endometrial-derived stem cells (hEnSCs) as multipotent accessible source of cells are known as useful cell candidates in the field of nerve tissue engineering. (scirp.org)
These results show that for the first time, human endometrial stem cells can be differentiated into Schwann cells in 2D and 3D culture. (scirp.org)
Human neural stem cells (NSCs) are inherently tumor---tropic, localizing to tumor foci following intracerebral or intravenous administration in preclinical models of brain tumors, breast carcinoma, and neuroblastoma. (selectbiosciences.com)
Embryonic stem cells are intriguing because they can differentiate into any of the specialized tissue cells of the body. (latimes.com)
This may allow scientists to culture healthy tissue cells in the lab to replace damaged ones in the human body. (latimes.com)
State-of-the art technologies are used to investigate global molecular changes that arise during neuronal fate specification and differentiation from pluripotent embryonic stem (ES) cells. (yale.edu)
Exploring the genetic, molecular and biological features of neural differentiation process from embryonic and patient-specific stem cells may offer an unprecedented chance to understand normal and abnormal human brain development and diseases, with implications for drug development and potential cellular replacement therapy. (yale.edu)
Initially, he established genetic cell reprogramming for generating iPS cells with the aim to model human diseases like Alzheimer's and Parkinson's disease and atypical Rett syndrome. (michaeljfox.org)
In this study, we investigated how higher-order chromatin structure modulates differential expression of the human INK4b-ARF-INK4a locus during progenitor cell differentiation, cellular ageing and senescence of cancer cells. (biomedcentral.com)
We found that INK4b and INK4a , but not ARF , are upregulated following the differentiation of haematopoietic progenitor cells, in ageing fibroblasts and in senescing malignant rhabdoid tumour cells. (biomedcentral.com)
The researchers cultured mouse C2C12 cells and human primary mesenchymal (HUCPVC) cells in the presence or absence of rhBMP-2 with increasing doses (0-100 μM) of the bisphosphonate zoledronate (ZOL). (jcda.ca)
Stem cells, and in particular human embryonic stem (ES) cells, have earned a reputation for being labour intensive and difficult to grow and control in culture. (ddw-online.com)
F urther complicating matters, human ES cells are typically co-cultured with feeder layers of mouse fibroblast cells. (ddw-online.com)
Several growth factors have been identified that promote growth of human ES cells in culture, most notably basic fibroblast growth factor (bFGF). (ddw-online.com)
However, the use of bFGF for human ES cell culture, particularly at the high levels used by some for 'feeder-free' culture, must be considered carefully, as bFGF may drive ES cells towards differentiation. (ddw-online.com)
Xeno-free media that do not rely upon high levels of bFGF have been developed that contain only humanised or synthetic components and are engineered to support the undifferentiated growth and expansion of human ES cells. (ddw-online.com)
Use of progenitor cell lines can eliminate the need to culture challenging human ES cells. (ddw-online.com)
Progenitor cells are similar to embryonic stem cells in their capacity to differentiate into various cell types. (ddw-online.com)
For example, neural progenitor cells derived from a human ES cell line are easily propagated and require less handling than human ES cells. (ddw-online.com)
The human sex chromosomes, called X and Y, are structures in human cells made up of tightly bound deoxyribonucleic acid, or DNA, and proteins. (asu.edu)
The global Human Embryonic Stem Cells market size was valued at USD 494.7 million in 2015. (grandviewresearch.com)
Controlled differentiation that can be achieved in tissue committed cells through the co-culturing with basic fibroblast growth factor is expected to induce growth. (grandviewresearch.com)
Human embryonic stem cells (hESCs) and their derived products offer great promise for targeted therapies and drug screening, however, the hESC differentiation process of mature neurons is a lengthy process. (northumbria.ac.uk)
Statement of significance: Human embryonic stem cells (hESC) are used for investigating the complex mechanisms involved in the development of specialized biological cells and organs. (northumbria.ac.uk)
Accelerating the hESCs' differentiation process can considerably benefit the clinical utilization of these cells. (northumbria.ac.uk)
Our results show that acoustic dose alters the extracellular matrix and physiological activity-related gene expression, which indicates that the acoustic stimulation is an important tool for regulating the stem cells' differentiation processes in cell therapy. (northumbria.ac.uk)
Here we show the bioenergetics profile of human stem cells of embryonic origin differentiating along the hepatic lineage. (ku.dk)
Our study reveals especially the transition between hepatic specification and hepatic maturation as dependent on mitochondrial respiration and demonstrates that even though differentiating cells are primarily dependent on glycolysis until induction of hepatocyte maturation, oxidative phosphorylation is essential at all stages of differentiation. (ku.dk)
Defined culture of human embryonic stem cells and xeno-free derivation of retinal pigmented epithelial cells on a novel, synthetic substrate. (ca.gov)
Human embryonic stem cells (hESCs) can generate an unlimited source of RPE for cellular therapies, and clinical trials have been initiated. (ca.gov)
Most of the tumors represent hamartomas and, in many organs, resemble embryonic cells, suggesting that the defect occurs at an early stage in life. (medscape.com)
The UCSF-led team made its discovery by studying the impact of smoke on human embryonic stem cells as they differentiated, or specialized into various cell types, in the culture dish. (ucsf.edu)
They also established that at least some of the impact was mediated through several molecular pathways known to play a role in differentiation.In one of the pathways, the toxins dramatically increased the activity of a key gene that keeps embryonic stem cells in an undifferentiated state, suggesting that its disruption might be responsible for much of the delay seen in embryonic development. (ucsf.edu)
First, they extended the analysis of gene activity in umbilical cord stem cells previously examined by their University of Connecticut co-authors, determining that tobacco smoke increased the activity of genes that delay the development of mesoderm - the layer of tissue that gives rise to blood, musculoskeletal and cardiovascular systems - as well endoderm and ectoderm, the two other layers of embryonic cells that give rise to the tissues of the body. (ucsf.edu)
Next, they exposed human embryonic stem cells in the culture dish either to tobacco smoke or nicotine at concentrations found in fetal blood. (ucsf.edu)
They discovered that both nicotine and non-nicotine components of tobacco smoke increased the activity of genes that hold embryonic stem cells in a pluripotent, or undifferentiated, state. (ucsf.edu)
They also highlight the power of human embryonic stem cells as a model of human development. (ucsf.edu)
This iPSC line upon pancreatic b-cell differentiation serves for an easy readout, allowing us to determine Alpha and Beta cell fates in live cells at earliest time points during differentiation, sort these populations and analyse their fate, plasticity and the use drugs to influence this fate decisions. (helmholtz-munich.de)
In essence, the simple cells of the embryo will transform into more complex cells, allowing for the formation of human tissues as well as other cell types. (infertilityivfhouston.com)
New research says studying both adult and embryonic stem cells can benefit medical science, but banning the study of either type could harm studies of the other. (sciencedaily.com)
The researchers analyzed more than 2,000 scientific papers and found adult stem cells are not replacing human embryonic stems cells in the laboratory. (sciencedaily.com)
He notes the science behind adult stem cells that can be "reprogrammed," called human induced pluripotent stem cells (hiPSCs), is still in its infancy, having become widely available in 2007. (sciencedaily.com)
Pluripotent stem cells are those capable of differentiating into any type of tissue, hence the attractiveness of embryonic stem cells, or hESCs, also called ES cells, which are also pluripotent. (sciencedaily.com)
They found the proportion of papers using human adult and human embryonic stem cells together is growing faster than those using adult stem cells alone. (sciencedaily.com)
In 2008, only 15 or 5.1 percent of all papers examined in the study reported using adult stem cells, and only three of those papers combined the use of human adult and human embryonic stem cells. (sciencedaily.com)
ROR2 (Receptor Tyrosine Kinase-like Orphan Receptor 2) is broadly expressed during embryonic development and can be found in cells of all three germ layers as well as in most organ tissues. (rndsystems.com)
Promotes differentiation of cardiomyocytes from human pluripotent stem cells (PSCs) that have been induced to mesoderm by addition of Activin A and/or BMP4 (Ren et al. (stemcell.com)
Induces the differentiation of human PSC-derived alveolar epithelial type II (AETII) to AETI cells (Ghaedi et al. (stemcell.com)
Human embryonic stem cells (hESC) are undifferentiated multiple-function cells. (eurekalert.org)
Since 2007, a handful of research teams across the world have been capable of reprogramming human adult cells into induced pluripotent cells (iPSC), which have similar characteristics and potential to human embryonic stem cells (hESC). (eurekalert.org)
This kind of reprogramming makes it possible to reform all human cell types without the ethical restrictions related to using embryonic stem cells. (eurekalert.org)
The researchers have successfully rejuvenated cells from elderly donors, some over 100 years old, thus demonstrating the reversibility of the cellular aging process. (eurekalert.org)
The researchers proved that the iPSC cells thus obtained then had the capacity to reform all types of human cells. (eurekalert.org)
They have the physiological characteristics of "young" cells, both from the perspective of their proliferative capacity and their cellular metabolisms. (eurekalert.org)
Using this new "cocktail" of six factors, the senescent cells, programmed into functional iPSC cells, re-acquired the characteristics of embryonic pluripotent stem cells. (eurekalert.org)
The rejuvenated iPSC cells were again differentiated to adult cells and compared to the original old cells, as well as to those obtained using human embryonic pluripotetent stem cells (hESC). (eurekalert.org)
Therefore, our aims are to develop cellular models of human neuronal aging and degeneration, to investigate underlying mechanisms responsible for dysfunction in neurons and neural stem cells in the aging and diseased brain and to dissect the regulation of normal neurogenesis. (lu.se)
We subsequently added Eagle minimum essential medium supplemented with 2% fetal calf serum and incubated the cells at 37°C. Postinfection cytopathic effects were observed by using an inverted light microscope (Nikon, Melville, NY, USA), and cellular morphologic changes were recorded. (cdc.gov)
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
Although possible, these strategies are often complicated to transfer to human cells due to technical issues (9 ), and only a few successful cases have been described ( 10, 11 ). (lu.se)
Here we describe how this system can be used for human embry- onic stem cells. (lu.se)
Sozzi E, Nilsson F, Kajtez J, Parmar M, Fiorenzano A. Generation of Human Ventral Midbrain Organoids Derived from Pluripotent Stem Cells . (lu.se)
Based on their origin, there are two main types of stem cells - embryonic stem cells (ES cells) and postnatal or adult stem cells (AS cells). (bvsalud.org)
Embryonic stem cells are stem cells derived from the inner cell mass of an early, preimplantation stage embryo known as a blastocyst. (bvsalud.org)
The possible donor-host rejection of human ES cells is another concern 3 . (bvsalud.org)
To date, four types of human dental stem cells have been isolated and characterized: (i) dental pulp stem cells (DPSCs) 12 (ii) stem cells from exfoliated deciduous teeth (SHED) 21 (iii) stem cells from apical papilla (SCAP) 31 (iv) periodontal ligament stem cells (PDLSCs) 28 . (bvsalud.org)

7 Thawing and Seeding of Frozen Mouse Embryonic Fibroblasts ( Meng-Jiao Shi, Maria Borowski, and Kimberly Stencel ). (ecampus.com)
To define better cellular events that are specifically vulnerable to WRN deficiency, I used RNA interference (RNAi) to silence the expression of Wrn gene and other RecQ helicases, linked to different clinical phenotypes, in primary human fibroblasts. (yale.edu)
Lately, his group employed lineage-specific transcription factors for reprogramming cellular identity and generating therapeutic relevant neuronal subtypes from conversion of skin fibroblasts. (michaeljfox.org)
Then, his group identified a minimal combination of three transcription factors (Mash1, Nurr1 and Lmx1a) able to directly convert model and human fibroblasts into functional dopaminergic neurons. (michaeljfox.org)
B) INK4b and INK4a , but not ARF , are upregulated in ageing human diploid fibroblasts (HDFs). (biomedcentral.com)

Our goal is to improve beta cell differentiation and function, provide platforms for disease modeling and drug screening and, as ultimate goal, help to overcome remaining hurdles for stem cell-derived beta cell replacement therapies in diabetic patients. (helmholtz-munich.de)
To use this knowledge to improve beta cell differentiation protocols. (lu.se)

We have performed a combinatorial study on both hESCs and hiPSCs to compare the effects of enzymatic vs. mechanical passaging, and feeder-free vs. mouse embryonic fibroblast feeder substrate, on the genetic and epigenetic stability and the phenotypic characteristics of hPSCs. (ca.gov)
Faculty in our genetics and cell biology group investigate the genetic basis for animal growth, disease resistance, and nutrient efficiency, and the mechanisms underlying nutrient uptake, cellular differentiation, and lipid secretion. (umd.edu)
My research interest focuses on how genes and genetic mechanisms contribute to childhood developmental or late-onset disorders of the human nervous system. (yale.edu)
The discovery of the structure of DNA also made future research possible into the molecular basis of embryonic development, genetic disorders, and gene manipulation. (asu.edu)
Our strength is the generation genetic mouse and human stem cell models including gene knock out models but also fluorescent reporter lines. (helmholtz-munich.de)
In the first day of embryonic development, the sperm fertilizes an egg, fusing the separate genetic material. (infertilityivfhouston.com)

My PhD focussed on investigating the distributions and possible functions of nitric oxide/nitric oxide synthases in the physiology and pathology of a variety of tissues and organs and I was fortunate enough to publish some of the first papers on the involvement of these molecules in human diseases, notably in the cardiovascular system. (nottingham.ac.uk)
Applicability for inducing differentiation into a wide range of tissues is attributive to the largest share of regenerative medicine. (grandviewresearch.com)
Extracellular vesicle DNA from human melanoma tissues contains cancer-specific mutations. (gu.se)
It has been a long-standing challenge to decipher the mechanisms by which thyroid hormone regulates such a wide range of cellular processes in so many different tissues. (jci.org)
The use of olfactory progenitors as a cell-based therapy for Parkinson's disease (PD) would allow harvest without invasive surgery, provide an autologous cell population, eliminate need for immunosuppression and avoid the ethical concerns associated with embryonic tissues. (scirp.org)
According to the cancer stem cell (CSC) concept, the cellular hierarchy in normal tissues is preserved in a number of hematological and solid malignancies. (medscape.com)

Retinoic acid plays a critical role in development, cellular growth, and differentiation. (wikipedia.org)
This gene may play a role in embryonic development and epithelial cell differentiation. (wikipedia.org)
We then analyzed the temporal and spatial expression of rab11a gene in embryonic development by RT-PCR and whole mount in situ hybridization. (researchsquare.com)
It may also give researchers a method of studying disease processes at the cellular level, yielding insights that could contribute to the development of other therapies. (latimes.com)
As such, the Department's research programs extend beyond traditional areas of animal science and include investigation into the molecular, cellular and biochemical basis of animal reproduction and development. (umd.edu)
Vania Broccoli, PhD, is a developmental neurobiologist who has been investigating some crucial molecular mechanisms shaping brain development and neuronal differentiation. (michaeljfox.org)
Variation in the policies across the globe is expected to impact the global human embryonic stem cell market development. (grandviewresearch.com)
provide evidence that thyroid hormone receptors are essential for the formation of a population of parvalbuminergic neurons in the anterior hypothalamus, linking, for the first time, impaired thyroid hormone signaling during development to cellular deficits in the hypothalamus. (jci.org)
Transcription factors (nurr1, pitx3 and lmx1a) that promote embryonic mouse or chicken dopaminergic development were employed to determine if they would modulate lineage restriction of these adult human progenitors. (scirp.org)
Experiments in human cell lines, mice, Drosophila, and Caenorhabditis elegans have shown that endonuclease G (ENDOG) plays a protective role against the development of TSC. (medscape.com)
Scientists have gained insight into how second-hand tobacco smoke damages the earliest stages of human embryonic development. (ucsf.edu)
We hope the findings will be a launching pad for further investigations on the impact on fetal development at the cellular level. (ucsf.edu)
We'd like to take a moment to consider what an embryo is and why timing is crucial when it comes to embryonic development and the IVF process. (infertilityivfhouston.com)
By the forth day of embryonic development, differentiation tends to occur. (infertilityivfhouston.com)
By the fifth or sixth day of embryonic development, a blastocyst will have formed. (infertilityivfhouston.com)
Each phase in human development has different susceptibilities to the effects of environmental toxicants. (cdc.gov)
Understanding the mechanisms underlying embryonic beta cell development will be a prerequisite for the development of a universally available cell replacement therapy in diabetes. (lu.se)

Hamartin and tuberin are thought to act synergistically to regulate cellular growth and differentiation. (medscape.com)
The practice of dentistry is likely to be revolutionized by biological therapies based on growth and differentiation factors that accelerate and/or induce a natural biological regeneration. (bvsalud.org)

The molecular events leading to human embryonic stem cell (hESC) differentiation are the subject of considerable scrutiny. (northwestern.edu)

Acceleration mechanisms for neural differentiation have been explored by bulk RNA sequencing, quantitative polymerase chain reaction (qPCR) and immunostaining. (northumbria.ac.uk)
if human embryonic, only NIH approved human embryonic stem cell lines may be used) as a source for making pancreatic islets, and to determine the basic mechanisms underlying beta cell regeneration in the adult as a basis for producing new cellular therapies for diabetes. (nih.gov)
The mechanisms of cellular energy sensing and AMPK-mediated mTORC1 inhibition are not fully delineated. (medscape.com)
The multidisciplinary consortium modelled and quantified the dynamics and mechanisms of epigenetic modulation at the cellular and organismal level. (ucl.ac.uk)
To understand the molecular and cellular mechanisms that control beta cell maturation and function in the embryonic and adult pancreas. (lu.se)

Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
This study suggests that specific transcription factors and treatment with morphogens can restrict human adult olfactory-derived progenitors to a dopaminergic neuronal lineage. (scirp.org)

Neural differentiation of the hESCs is greatly accelerated after application of the acoustic stimulations. (northumbria.ac.uk)
This study develops a highly effective acoustic stimulator working at ∼20 MHz to investigate what roles do acousto-mechanical stimuli play in the differentiation of hESCs. (northumbria.ac.uk)
In this study, we investigated the maintenance of hESCs and their differentiation into RPE using Synthemax II-SC, which is a novel, synthetic animal-derived component-free, RGD peptide-containing copolymer compliant with good manufacturing practices designed for xeno-free stem cell culture. (ca.gov)
This report demonstrates that Synthemax II-SC supports long-term culture of H9 and H14 hESC lines and permits efficient differentiation of hESCs into functional RPE. (ca.gov)

Finally, they assessed gene expression in three stem cell differentiation pathways, known as Notch, canonical Wnt and TGF-β. (ucsf.edu)
Proteomics is emerging as a powerful tool to identify the signaling complexes and pathways that control multi- and pluri-potency and SC differentiation. (medscape.com)

He is Executive Editor of Journal of Cellular Biochemistry , Journal of Cellular Physiology , Critical Reviews in Eukaryotic Gene expression , and Molecular Biology Reports , and has served on numerous Editorial Boards. (ecampus.com)

Triple-negative breast cancer (TNBC), which lacks the expression of the estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2), accounts for 10-15% of breast cancers [ 2 ]. (nature.com)
Expression of the INK4b-ARF-INKa locus during cellular ageing and differentiation . (biomedcentral.com)
Thyroid hormone acts through the intracellular thyroid hormone receptor (TR), which belongs to the nuclear receptor family and acts as a ligand-regulated transcription factor ( 4 , 5 ), eliciting cellular responses by binding to and regulating the expression of target genes. (jci.org)

Stem cell research is, in part, a quest to understand cellular differentiation, the process by which a human being develops from one fertilized cell into a multicellular organism composed of over 200 different cell types - for example muscle, nerve, blood cell, or kidney. (jcpa.org)
Cellular differentiation begins with the fertilized egg which serves as the identifying characteristic of an embryonic stem cell. (jcpa.org)
In 2009, in a major reversal of U.S. policy, President Obama signed an executive order pledging to "vigorously support" embryonic stem cell research. (jcpa.org)
We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)
Additionally, RAD18 functions beyond DNA repair and may regulate many other key biological processes, such as chromatin strengthening, cell survival/death, stemness, and differentiation. (nature.com)
11 Human Embryonic Stem Cell Culture on BD Matrigel™with mTeSR®1 Medium ( Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski ). (ecampus.com)
Single-molecule analysis reveals changes in the DNA replication program for the POU5F1 locus upon human embryonic stem cell differentiation. (neurotree.org)
3. developed new embryonic stem cell-based approaches to screening of small candidate molecules for therapeutic applications in human diseases. (europa.eu)
Generation of human islet cell ty. (unige.ch)
The primary thrust of the Genetics and Cell Biology Group is to illuminate the molecular and cellular basis of complex biological systems using a multi-organismal and multi-faceted approach. (umd.edu)
During progenitor cell differentiation and ageing, PcG silencer EZH2 attenuates, causing loss of PRC binding and transcriptional activation of INK4b and INK4a . (biomedcentral.com)
Particularly, pathological and toxicology modeling can be performed by the provision of physiological models for different human cell types. (grandviewresearch.com)
Single-Cell Transcriptomics Reveals that Differentiation and Spatial Signatures Shape Epidermal and Hair Follicle Heterogeneity. (crossref.org)
The Human Cell Atlas. (crossref.org)
The acoustic stimulator fabricated by using the PCB is a promising tool in regulation of stem cell differentiation process applied in cell therapy. (northumbria.ac.uk)
WD repeat domain 5 (WDR5) is a member of the WD repeat protein family, which is involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. (thermofisher.com)
We defined this cell population as "specified," as it is committed toward differentiation. (northwestern.edu)
How does the same hormone stimulate the differentiation of an embryonic neuroblast but trigger an entirely different response in an adult liver cell? (jci.org)
Anderson, L. and Caldwell, M.A. (2007) Human neural progenitor cell transplants into the subthalamic nucleus lead to functional recovery in a rat model of Parkinson's disease. (scirp.org)
By 2010, some 161 of 574 or 28 percent of papers reported on studies of both cell technologies, and 62.1 percent of those papers paired adult and embryonic cell lines. (sciencedaily.com)
Because use of the two cell types has become so intertwined, any federal policy that would deny funding for embryonic stem cell research "would derail work with a nascent and exciting technology," says Owen-Smith. (sciencedaily.com)
If federal funding stops for human embryonic stem cell research, it would have a serious negative impact on adult stem cell research, says Stanford University bioethicist Christopher Scott, one of the paper's co-authors. (sciencedaily.com)
Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)

This feature occurs because a shortage of the ALX4 transcription factor caused by deletion of the gene disrupts several cellular processes and impairs proper bone formation (ossification). (medlineplus.gov)

Murine Rab11a and Rab11b were found to localize in distinct cellular compartment, suggesting functional differentiation [9]. (researchsquare.com)
LINE-1 retrotransposons drive human neuronal transcriptome complexity and functional diversification. (lu.se)

This study optimized conditions for the differentiation of hONPs towards dopaminergic neurons. (scirp.org)

Cellular Physiology and Biochemistry (2018) 46 (2): 546-560. (karger.com)

These experiments suggest that Synthemax II-SC is a suitable, defined substrate for hESC culture and the xeno-free derivation of RPE for cellular therapies. (ca.gov)

Rapid advancements in this vertical include protocols for directed differentiation, defined culture systems, demonstration of applications in drug screening, the establishment of various disease models, and evaluation of therapeutic potential in treating incurable diseases. (grandviewresearch.com)

The RNA Helicase DDX6 Controls Cellular Plasticity by Modulating P-Body Homeostasis. (neurotree.org)

A) Organization of the human INK4b-ARF-INK4a locus (not drawn to scale), encoding three distinct proteins, p15 INK4b , p14 ARF and p16 INK4a . (biomedcentral.com)

The findings reinforce the importance of screening iPSCs for potentially harmful DNA mutations before using them for therapeutic purposes, said lead investigators Ali Torkamani, Ph.D., director of genome informatics at STSI, and Kristin Baldwin Ph.D., the study's co-lead investigators and associate professor of molecular and cellular neuroscience at the Dorris Neuroscience Center at TSRI. (sciencedaily.com)

Here, we highlight the pre---clinical characterization, safety and therapeutic efficacy study results that led to permission from the U.S. Food and Drug Administration to initiate a first---in---human phase I study using genetically modified NSCs for tumor selective enzyme/prodrug therapy in recurrent glioma patients (clinicaltrials.gov ID # NCT01172964). (selectbiosciences.com)

The Burroughs Wellcome Fund serves and strengthens society by nurturing a diverse group of leaders in biomedical sciences to improve human health through education and powering discovery in frontiers of greatest need. (bwfund.org)

Anatomy57

Organisms8

Diseases8

Chemicals and Drugs85

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Phenomena and Processes68

Disciplines and Occupations2

Information Science4

Early embryonic development3

Mouse embryonic stem2

Surrounding embryonic stem cel2

Recycling during embryonic deve1

Vitro8

Mesenchymal stem1

Proliferation5

Self-renewal5

Cells99

Fibroblasts5

Improve beta cell differentiation2

Genetic6

Tissues6

Development17

Growth and differentiation2

HESC differentiation1

Mechanisms5

Lineage2

HESCs4

Pathways2

Gene expression1

Expression3

Cell23

Processes1

Functional2

Neurons1

Biochemistry1

Therapies1

Protocols1

Plasticity1

Locus1

Neuroscience1

Characterization1

Biomedical1