• It also discusses the effects of ooplasmic transfer on oocytes and embryos, and mtDNA. (fertiligy.com)
  • Ooplasm donation is a technique where donor oocytes are used to make embryos. (fertiligy.com)
  • Herein, we aimed to investigate whether age-associated accumulation of advanced glycation end-products (AGEs) in oocytes impairs the recovery of embryos from cryopreservation-induced mitochondrial dysfunction/damage. (bvsalud.org)
  • Mouse eight-cell stage embryos developed in vitro were vitrified and warmed and incubated up to the blastocyst stage. (bvsalud.org)
  • The direct transfer methods of frozen-thawed, in vivo- derived, and in vitro- produced (IVF) bovine embryos using 1.5 M ethylene glycol (EG) with or without sucrose (SUC) are used widely under on-farm conditions, not only in Japan but also globally. (go.jp)
  • The pregnancy rate with direct transfer of frozen-thawed bovine embryos in either EG or GLY-SUC has been found to not differ from conventional freezing with GLY and traditional dilution techniques. (go.jp)
  • We performed mitochondrial DNA Next Generation Sequencing and determined that one mtDNA variant harbored by EPCs was present in oocytes, preimplantation embryos and somatic tissues over three generations in our mini-pig model indicating the potential germline origin of EPCs. (oncotarget.com)
  • iii) We are examining how the three-dimensional organization of the genome changes during differentiation and reprogramming processes, how transcriptional networks and lncRNAs regulate 3D genome organization, and how 3D genome organization in turn influences gene expression, chromatin states, and lncRNA function. (ucla.edu)
  • The quality of mitochondria and the numbers of copies of its genome, mitochondrial DNA (mtDNA), in oocytes are beginning to be considered, along with other factors, as indicators of oocyte quality, especially in the context of aging [ 5 - 11 ]. (oncotarget.com)
  • The oocyte has two mtDNA haploplasmy states - mitochondrial heteroplasmy in the donor, and mitochondrial homoplasmy in the recipient oocyte. (fertiligy.com)
  • PACRG is a protein found in the mitochondrial sheath of the oocyte prior to fertilization and at the tail of the sperm after exposure to oocyte extract. (fertiligy.com)
  • During ovulation, a newly fertilized oocyte carries mtDNA from both parents. (fertiligy.com)
  • Fetal germ cells (FGCs) are embryonic precursors of mature gametes, i.e. sperm or oocytes. (nature.com)
  • Male-factor infertility can be overcome by a technique called intracytoplasmic sperm injection (ICSI), which directly injects a sperm into an oocyte, and is one of the most widely used treatments in the fertility clinic [ 3 ]. (oncotarget.com)
  • They showed that opposing gradients of bone morphogenetic protein (BMP) and Nodal, two transforming growth factor family members that act as morphogens, are sufficient to induce molecular and cellular mechanisms required to organize, in vivo or in vitro, uncommitted cells of the zebrafish blastula animal pole into a well-developed embryo. (wikipedia.org)
  • Actin networks are responsible for driving ooplasm segregation in zebrafish oocytes. (fertiligy.com)
  • To address these questions, we cultured 7-week male FGCs from testis in vitro with or without its antagonist LDN-193189 2HCl (LDN), and analyzed the transcriptional and epigenetic differences at single-cell level between these two conditions. (nature.com)
  • Because ooplasms are genetically diverse, the oocytes can impose epigenetic modifications on the parental genomes. (fertiligy.com)
  • The Plath lab focuses on the epigenetic mechanisms that maintain pluripotency and control differentiation and reprogramming processes. (ucla.edu)
  • Progenitors are obtained by so-called direct reprogramming or directed differentiation and are also called induced somatic stem cells. (wikipedia.org)
  • The process involves sucking out the nucleus of a somatic (body) cell and injecting it into an oocyte that has had its nucleus removed Using an approach based on the protocol outlined by Tachibana et al. (wikipedia.org)
  • They form characteristic cell clusters in suspension culture that express a set of genes associated with pluripotency and can differentiate into endodermal, ectodermal and mesodermal cells both in vitro and in vivo. (wikipedia.org)
  • This meant that the cells can change their differentiation pathway. (wikipedia.org)
  • Finally, using an in vitro culture system of human FGCs, we showed that the BMP signaling pathway promoted the cell proliferation of FGCs, and regulated the WNT signaling pathway by orchestrating the chromatin accessibility of its ligand genes. (nature.com)
  • We establish a pH dependant oligomerisation pathway forming tetrameric DEC-205 using solution-based techniques and ultimately solved the 4.9 Å cryo-EM structure of the DEC-205 tetramer to identify the unfurling of the second lectin ring which enables tetramer formation. (uci.edu)
  • On the other hand, treatment for female-factor infertility is often restricted to in vitro fertilization, since ICSI does not appear to be an effective treatment for poor quality oocytes [ 4 ]. (oncotarget.com)
  • Similarly, the oocytes' actin networks generate pulling forces near the ooplasm-yellow granule interface. (fertiligy.com)
  • To demonstrate the power of this technology, we will generate a molecular disease fingerprint allowing differentiation between three clinically indistinguishable yet biochemically distinct disease pathways underlying the deadly brain cancer glioblastoma multiforme. (nih.gov)
  • Using in vitro and cellular techniques, we present a mVP40 assembly model highlighting two distinct oligomerization interfaces: the (N-terminal domain (NTD) and C-terminal domain (CTD)) in mVP40. (uci.edu)
  • Ooplasm controls many processes during the oocyte's maturation and activation. (fertiligy.com)
  • Within this context, we are studying six fundamental problems: (i) How do long-noncoding (lnc) RNAs build nuclear compartments and regulate chromatin changes during differentiation and reprogramming? (ucla.edu)
  • iv) We are aiming to understand how nutrients that vary with diet can regulate stem cell states and cell fate decisions, particularly those during development, with the ultimate goal to define dietary alterations that enable more accurate models of differentiation in the culture dish or lead to new treatments of pregnancy complications and diseases. (ucla.edu)
  • An increasing number of women are delaying childbirth, and, since oocyte quality declines dramatically after 35 years of age [ 1 , 2 ], more women are requiring assisted reproductive treatments. (oncotarget.com)
  • Expression of the pluripotent markers Sox2 and Oct4 , and the primitive germ cell markers Blimp1 and Stella were not detected. (oncotarget.com)
  • Gene expression profiles of adult renal corpusle isolated using sieving techniques. (nih.gov)
  • Without ooplasmic regulators, oocytes can be rendered of low quality. (fertiligy.com)
  • Female-factor infertility can be caused by poor oocyte quality and depleted ovarian reserves. (oncotarget.com)
  • The presence of Zp1 , Zp2 , Zp3 and Nobox were not detected, indicating that porcine EPCs are not indicative of mature primordial oocytes. (oncotarget.com)
  • Embryo freezing techniques have been developed over the last 40 years, allowing practical availability, and have become essential for cattle reproduction management under field conditions. (go.jp)
  • Embryo transfer entails many procedures and techniques, of which embryo freezing is an important component in bovine embryo transfer. (go.jp)