Differentiation murine leukemia cell line. Medical search. Frequent questions

Chronic myeloid leukemia (CML) results from BCR-ABL oncogene, which blocks CML cells differentiation and protects these cells from apoptosis. (jcancer.org)
Here, we determined the effect of Jiyuan oridonin A (JOA), an ent -kaurene diterpenoid compound, on the differentiation blockade in imatinib-sensitive, particularly, imatinib-resistant CML cells with BCR-ABL-T315I mutation by cell proliferation assay, apoptosis analysis, cell differentiation analysis, cell cycle analysis and colony formation assay. (jcancer.org)
CXCR7 agonist stimulated the migration of LTS derived fibroblasts significantly in vitro, with no significant influence on the cell proliferation and apoptosis. (bvsalud.org)
Yet another group of genes associated with cancer formation regulates programmed cell death, or apoptosis. (the-scientist.com)
The importance of apoptosis is exemplified by the vertebrate adaptive immune system, in which B lymphocytes, as they differentiate from their precursor cells, rearrange the DNA of immunoglobulin genes to generate a vast number of potential antibodies. (the-scientist.com)
Combined stimulation with the T helper cell type 2 cytokines interleukin (IL)-4 and IL-10 induces mouse mast cell apoptosis. (southernbiotech.com)
VSV infection triggered rapid differentiation of blood monocytes into immature dendritic cells as well as their apoptosis, which depended on caspase 3/7 activation. (karger.com)
The hypoxia-induced apoptosis, cell survival, and cell paracrine function of aged hBM-MSCs were investigated in vitro. (biomedcentral.com)
Through lentivirus-mediated upregulation of miR-10a and downregulation of KLF4 in aged hBM-MSCs in vitro, we revealed that miR-10a decreased hypoxia-induced cell apoptosis and increased cell survival of aged hBM-MSCs by repressing the KLF4-BAX/BCL2 pathway. (biomedcentral.com)
They also found that downregulation of miR10a/10b in clonal cells interfered with cell proliferation and enhanced cell apoptosis by activating the NF-κB-dependent p53 pathway [ 13 ]. (biomedcentral.com)
overexpression of these genes decreased proliferation or induced apoptosis of human MLL-AF4 + ALL cell lines, respectively. (ashpublications.org)
The degree of methylation affects the expression of target genes and has a significant impact on cell proliferation, apoptosis, cytokine secretion, and cell activity ( 6 , 7 ). (frontiersin.org)
Calcitriol elicits anti-tumor effects mainly through the induction of cancer cell apoptosis, cell cycle arrest, differentiation, angiogenesis and the inhibition of cell invasiveness by a number of mechanisms. (jcancer.org)
The broad spectrum anti-tumor effects of calcitriol and analogs are mostly based on inhibition of cancer cell proliferation and invasiveness, induction of differentiation and apoptosis, and promotion of angiogenesis. (jcancer.org)

Chronic myeloid leukemia (CML) is a clonal expansion of the progenitor hematopoietic stem cells arising from the existence of the fusion BCR-ABL oncogene, that represents 15%-20% of the newly diagnosed cases of leukemia patients [ 1 - 2 ]. (jcancer.org)
MDS is a clonal disorder of myeloid stem cells. (medscape.com)
1 The BCR-ABL1 fusion protein is a constitutively active tyrosine kinase and triggers a cascade of aberrant downstream signaling pathways leading to clonal outgrowth of CML cells and subsequent disease manifestation. (haematologica.org)
Myelodysplastic syndrome (MDS) in childhood encompasses a diverse group of bone marrow disorders that share a common clonal defect of stem cells and that result in ineffective hematopoiesis with dysplastic changes in the marrow. (medscape.com)
We discuss the rationale of various anti-fibrogenic treatment strategies targeting the clonal hematopoietic stem/progenitor cell, aberrant signaling pathways, fibrogenic cytokines, and the tumor microenvironment. (haematologica.org)
The tumor cells are clonal and characterized by an increased expression of several cellular oncogenes. (biomedcentral.com)
The transformed B cell population consists of CD19 + pre-BCR + CD127 + CD43 + CD93 + precursor B cells and are clonal. (biomedcentral.com)

Transgenic reporter mouse embryonic stem cells were used in this study. (siu.edu)
To promote adhesion of mouse embryonic stem cells and to initiate integrin-based mechanical signaling, the extracellular matrix protein fibronectin was used. (siu.edu)
In the presence of fibronectin and a small molecule called leukemia inhibitory factor (LIF), which is known to maintain pluripotency and self-renewal, mouse embryonic stem cells responded by exhibiting expressions of both GFP and DsRed. (siu.edu)
We have used RT-PCR to screen pluripotent murine embryonic stem cells to identify receptor tyrosine kinases (RTKs) potentially involved in the determination or differentiation of cell lineages during early mouse development. (silverchair.com)
The potential use of embryonic stem cells (ESCs) for cell replacement therapies is limited by ethical concerns and the technical hurdles associated with their isolation from human embryos. (biomedcentral.com)

B lymphoma Moloney murine leukemia virus insertion region 1 homolog (BMI-1) is an oncogene which functions by regulating P16 and P19 cell cycle inhibitor genes and is also associated with erythroplakia and tongue cancer. (cancertools.org)
Some of the first iPSCs were generated with Moloney murine leukemia virus (MMLV)-derived retroviruses. (addgene.org)

Monoclonal antibodies reactive with myeloid precursor cells in the bone marrow or cells undergoing early myeloid differentiation are capable of detecting antigens that may then appear at discrete stages of myeloid maturation or on cells from some patients with myeloid and/ or monocytic leukemia. (duke.edu)
8. Lui O, Mak N, Leung K. Conjugated linoleic acid induces monocytic differentiation of murine myeloid leukemia cells. (southernbiotech.com)
In addition to TAMs, solid tumors are also infiltrated by immunosuppressive, immature myeloid progenitor cells, commonly referred to as monocytic or polymorphonuclear myeloid-derived suppressor cells (M/PMN-MDSC) ( 11 - 13 ). (frontiersin.org)
Furthermore, a pioneering research by Ferrari (31) demonstrated that in microglia and monocytic Piroxicam (Feldene) IC50 cells LPS induces the discharge of ATP, recommending its participation in LPS-driven IL-1 secretion. (conferencedequebec.org)

EVs were isolated from the serum of acute myeloid leukemia (AML), acute lymphoid leukemia (ALL), chronic lymphoid leukemia (CLL) patients, and healthy volunteers. (nature.com)
Altered expression of this gene or a change in the subcellular localization of its protein is associated with some cases of acute myeloid leukemia and colorectal cancer. (wikipedia.org)
Overexpression of HOXB6, along with the addition of MEIS1 protein, has been implicated in the development of acute myeloid leukemia (AML). (wikipedia.org)
Acute myeloid leukemia is a cancer of the blood cells, specifically the leukocytes. (wikipedia.org)
MDS disorders have been referred to as "preleukemias" because of their tendency to transform into acute myeloid leukemia (AML). (medscape.com)
By studying murine transgenic leukemia models developed in our laboratory, we are currently exploring the relationships between hematopoietic progenitor cell identities and the development and progression of acute leukemia. (lu.se)
The production of distinct blood cell types can be selectively adapted to meet an individual's acute needs, such as for instance the rapid and enhanced generation of innate immune cells following infection. (lu.se)
7 In CML and acute myeloid leukemia (AML), we and others have shown that IL-1 is a positive regulator of LSC, and blocking IL-1 signaling inhibits the LSC. (haematologica.org)
1 Mixed lineage leukemia (MLL) gene fusions are the molecular hallmark of infant acute lymphoblastic leukemia (ALL) and are present in tumor cells in up to 80% of patients. (ashpublications.org)
DNA (cytosine-5)-methyltransferase 3A (DNMT3A)-mutated acute myeloid leukemia (AML) has a poor prognosis, but the exact mechanism is still unclear. (frontiersin.org)
Acute myeloid leukemia (AML) is a common, heterogeneous, and aggressive hematopoietic malignancy, characterized by apparent genetic abnormalities ( 1 ). (frontiersin.org)
Acute Myeloid Leukemia (AML) is an aggressive hematological malignancy that relies on highly heterogeneous cytogenetic alterations. (mdpi.com)

This gene has also been shown to encourage the growth of granulocytes and monocytes, but at the cost of other blood cells. (wikipedia.org)
Generated to the HL60 promyelocytic cell line, it can react with all hematopoietic myeloid cells, granulocytes and normal monocytes, as well as leukemic cells from some patients with AML, AMML, and ALL. (duke.edu)
However, these immunotherapeutic approaches are hampered by the tumor-promoting nature of myeloid cells, including monocytes, macrophages, and neutrophils. (frontiersin.org)
Myeloid innate immune cells, such as granulocytes, monocytes, macrophages and dendritic cells (DCs), play an important role in cancer-cell recognition, initiation of inflammation and antitumor responses ( 2 ). (frontiersin.org)
Osteoclasts can be obtained by isolating primary bone marrow monocytes or by using macrophage cell lines. (sciforschenonline.org)

Our group found that the proliferation and differentiation ability of aged human bone marrow mesenchymal stem cells (hBM-MSCs) was decreased whereas cell senescence was increased [ 7 ]. (biomedcentral.com)
Different conditions can affect survival, proliferation, and differentiation of RAW264.7 cells. (sciforschenonline.org)
Cyclic GMP is important for normal cell proliferation and differentiation. (livonlabs.com)

An oncoprotein can cause the transformation of a normal cell into a tumor cell. (wikipedia.org)
Similar to MLL-AF4 + human ALL, pMIRH-128a - and pMIRH-130b -transplanted mice showed significant leptomeningeal tumor cell infiltration. (ashpublications.org)
Clonogenic assays were performed on leukemic and tumor cell lines to evaluate the in vitro antineoplastic activity of DAC. (biomedcentral.com)
Increasing the DAC concentration and duration of exposure produced a greater loss of clonogenicity for both human leukemic and tumor cell lines. (biomedcentral.com)
We have observed that DAC reduced the in vitro clonogenic potential in a dose-dependent manner of both human and murine leukemia and tumor cell lines. (biomedcentral.com)

3. Radu DL, Weksler ME, Bona CA. Maintenance of size and function of influenza virus hemagglutinin specific transgenic T-cell clone during life. (southernbiotech.com)
Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)

HOXB6 is a structural protein that has been shown to influence the growth and differentiation of the different blood lineages. (wikipedia.org)
Aberration occurs in a stem cell that can give rise to multiple lineages. (medscape.com)
This event explains the presence of multiple derangements observed in the bone marrow that involve several cell lineages. (medscape.com)

Risk-adapted therapies for AML, ALL, and CLL patients consist of chemotherapy with/without stem cell transplantation (SCT), but the rate of early failures and relapses is still unsatisfying. (nature.com)
For pediatric patients with refractory cytopenia, certain cytogenetic abnormalities, or malignant transformation, hematopoietic stem cell transplantation (HSCT) from a matched related or unrelated donor early in the course of the disease is the treatment of choice. (medscape.com)
We have established an influence of both compromised genomic maintenance (nuclear and mitochondrial), and alterations in external growth control to these processes, which also have direct relevance for the stability and potential reversibility of the aging stem cell state. (lu.se)
Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm caused by an acquired 9;22-chromosomal translocation in a hematopoietic stem cell (HSC) resulting in the expression of the BCR-ABL1 fusion protein. (haematologica.org)
The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell reprogramming factors and wait for cells to de-differentiate. (addgene.org)
In vivo, transplantation of miR-10a-overexpressed aged hBM-MSCs promoted implanted stem cell survival and improved cardiac function after MI. (biomedcentral.com)
The age of stem cell donors might significantly impact the recipient's endogenous responses [ 6 ]. (biomedcentral.com)
Effective methods to rejuvenate aged hBM-MSCs to improve their regenerative capability may be required to maximize the beneficial effects of stem cell therapy. (biomedcentral.com)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)
The nature of the stem cell substates and their relationship to commitment to differ- entiate and lineage selection can be elucidated in terms of a landscape picture in which stable states can be defined mathematically as attractors. (lu.se)
MDS is rare in childhood and may have a rapidly progressive course with an extremely poor prognosis without hematopoietic stem cell transplantation (HSCT). (medscape.com)
Hematopoietic stem cell transplantation remains the only curative therapeutic approach that reliably results in resolution of bone marrow fibrosis in patients with myelofibrosis. (haematologica.org)
3 Myelofibrosis (MF) refers to the Philadelphia chromosome ( BCR-ABL1 )-negative myeloproliferative neoplasm (MPN) originating at the level of the multipotent hematopoietic stem cell. (haematologica.org)
The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)

To evaluate the leukemogenic role of RUNX1-MT in ASXL1 -mutated cells, we co-expressed RUNX1 -MT (R135T) and ASXL1 -MT (R693X) in different cell lines and performed immunoblot, co-immunoprecipitation, gene expression microarray, quantitative RT-PCR, cell proliferation, differentiation, and clonogenic assays for in vitro functional analyses. (biomedcentral.com)
Primary Human bronchial epithelial cells when grown in vitro have a limited lifespan and begin to deviate both in phenotype and morphology, losing the plasticity required around passage 4 or 5, for air-liquid interface (ALI) differentiation. (cancertools.org)
The in vivo antineoplastic activity of DAC administered as an i.v. infusion was evaluated in mice with murine L1210 leukemia by measurement of survival time, and in mice bearing murine EMT6 mammary tumor by excision of tumor after chemotherapy for an in vitro clonogenic assay. (biomedcentral.com)
The in vitro antineoplastic action of DAC correlated well with its inhibition of DNA methylation and reactivation of TSG in human neoplastic cells. (biomedcentral.com)
We also observed a correlation between in vitro and in vivo concentrations needed to produce optimal responses in mice with L1210 leukemia or with EMT6 tumor. (biomedcentral.com)
The DNMT3A-mutated AML cells attenuated M1 macrophage polarization and resisted its killing effect in vitro and in vivo . (frontiersin.org)
Proliferative/expansion potential, phenotypical characteristics and functionality of the propagated CD19CAR T cells were analyzed in vitro and in vivo after 17-21 day ex vivo expansion. (biomedcentral.com)
We found that Akt inhibition did not compromise CD19CAR T cell proliferation and expansion in vitro, independent of the T cell subsets, as comparable CD19CAR T cell expansion was observed after culturing in the presence or absence of Akt inhibitor. (biomedcentral.com)
In vitro , the sprouting and migratory properties of primary endothelial cell cultures were reduced by inhibiting EZH2 through up-regulation of miR-101, siRNA-mediated knockdown of EZH2, or treatment with 3-Deazaneplanocin-A (DZNep), a small molecule inhibitor of EZH2 methyltransferase activity. (plos.org)

The innate immune system includes macrophages, dendritic cells (DC), and natural killer (NK) cells, which respond quickly to an immunological threat. (nature.com)
Aberrant high expression of B lymphocyte chemokine (BLC/CXCL13) by C11b + CD11c + dendritic cells in murine lupus and preferential chemotaxis of B1 cells towards BLC. (southernbiotech.com)
Splenic endothelial cell lines support development of dendritic cells from bone marrow. (southernbiotech.com)
that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)

The research has shown that HOX B6 is not expressed in hematopoietic stem cells located in the red bone marrow, which are the precursor cells to all types of blood cells, or primordial germ cells (PGCs), the precursor to cells passed on in each generation. (wikipedia.org)
Mice transduced with both ASXL1 and RUNX1 mutations enhanced inhibitor of DNA binding 1 (ID1) expression in the spleen, liver, and bone marrow cells. (biomedcentral.com)
As the affected cell lines continue to divide and to provide the marrow with dysplastic cells, bone marrow dysfunction becomes apparent. (medscape.com)
Aberrantly expressed cytokines in the bone marrow (BM) niche are increasingly recognized as critical mediators of survival and expansion of leukemic stem cells. (haematologica.org)
2 1 There is growing evidence to suggest that primitive CML cells affect the bone marrow (BM) niche, contributing to deregulated cytokine levels. (haematologica.org)
Aging is one of the key factors that regulate the function of human bone marrow mesenchymal stem cells (hBM-MSCs) and related changes in microRNA (miRNA) expression. (biomedcentral.com)
Previous studies proved that bone marrow (BM) stem cells play an important role in improving heart function and delaying cardiac remodeling [ 1 , 2 ] after ischemic damage through reducing fibrosis and increasing angiogenesis. (biomedcentral.com)
Establish stromal cell layers and culture hematopoietic progenitors in "Dexter-type" long-term bone marrow cultures. (stemcell.com)
Osteoclasts are multinucleated cells that are essential for bone resorption and regulate bone remodeling. (sciforschenonline.org)
Although bone marrow fibrosis is seen in a variety of malignant and non-malignant disease states, the deposition of reticulin and collagen fibrosis in the bone marrow of patients with myelofibrosis is believed to be mediated by the myelofibrosis hematopoietic stem/progenitor cell, contributing to an impaired microenvironment favoring malignant over normal hematopoiesis. (haematologica.org)
15]. The percentage of bone common of which are trisomy 8, mono- marrow blast cells for estimation of the Myelodysplastic syndrome (MDS) is somy 7 and 5q- [11]. (who.int)

A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)

However, studies have shown that the sustained activity of Akt progressively drives T cells toward terminal differentiation and diminished anti-tumor activity [ 11 ]. (biomedcentral.com)
We therefore hypothesized that manipulation of the magnitude of Akt activation may prevent terminal differentiation of CAR T cells as they are expanded to a therapeutic dose. (biomedcentral.com)

BMI-1 can therefore be used to delay cell senescence. (cancertools.org)
We demonstrated previously that miR-10a is significantly decreased in aged hBM-MSCs and restoration of the miR-10a level attenuated cell senescence and increased the differentiation capacity of aged hBM-MSCs by repressing Krüpple-like factor 4 (KLF4). (biomedcentral.com)
We also found that miR-10a increased differentiation and decrease senescence in old hBM-MSCs through the target gene Krüpple-like factor 4 (KLF4) [ 7 ]. (biomedcentral.com)
However, other studies have suggested that miR-10a may have additional effects other than regulating cell senescence and differentiation. (biomedcentral.com)

We found that JOA at lower concentration significantly inhibited the proliferation of CML cells expressing mutant BCR-ABL (T315I mutation included) and wild-type BCR-ABL, which was due to that JOA induced the cell differentiation and the cell cycle arrest at G0/G1 phase. (jcancer.org)
BMI-1 is thought to repress, p16(Ink4a), a cyclin-dependent kinase inhibitor and tumor suppressor that induces cell cycle arrest at the Gap 1 phase. (cancertools.org)

The expression of RUNX1 mutant in ASXL1 -mutated myeloid cells augmented proliferation, blocked differentiation, and increased self-renewal activity. (biomedcentral.com)
This invention is a murine monoclonal antibody used to detect cell membrane antigens specific for human myeloid cells. (duke.edu)
Despite the arsenal of defense strategies against foreign invaders, myeloid cells succumb to the instructions of an established tumor. (frontiersin.org)
Interestingly, the most primordial defense responses employed by myeloid cells against pathogens, such as complement activation, antibody-dependent cell cytotoxicity and phagocytosis, actually seem to favor cancer progression. (frontiersin.org)
In this review, we discuss how rudimentary defense mechanisms deployed by myeloid cells can promote tumor progression. (frontiersin.org)
Hence, myeloid cells play a dual role in cancer as they can initiate antitumor responses and communicate with cells of the adaptive immune system, but also promote local inflammation leading to chronic cancer-associated inflammation ( 5 , 6 ). (frontiersin.org)
Nevertheless, the majority of scientific discoveries points toward a more tumor-supporting role for myeloid cells in the TME. (frontiersin.org)
The innate immune response by myeloid cells occurs as a succession of events starting at signaling through cytosolic or surface PRRs, followed by effector responses including the release of cytokines, reactive oxygen species (ROS), reactive nitrogen species (RNS), antibacterial peptides and degranulation ( Figure 1 ). (frontiersin.org)
PRR on myeloid cells can be triggered by pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs), but also indirectly by secondary mechanisms such as complement activation and circulating antibodies (Abs), resulting in cytolytic and phagocytic effector mechanisms. (frontiersin.org)

In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)

Mechanistically, the cell differentiation mediated by JOA may be originated from the inhibition of BCR-ABL/c-MYC signaling in CML cells expressing wild-type BCR-ABL and BCR-ABL-T315I. (jcancer.org)
Knockdown of miR-128a and/or miR-130b significantly impaired proliferation of human MLL-AF4 + cell lines, and inhibition of miR-130a significantly impaired disease initiation by the pediatric MLL-AF4 + SEM ALL cell line in immunodeficient NOD- scid IL2Rgamma null (NSG) mice. (ashpublications.org)
The reactivation of TSGs and inhibition of DNA methylation by DAC were investigated by reverse transcriptase-PCR and Line-1 assays. (biomedcentral.com)
The reactivation of the TSGs ( p57KIP2 in HL-60 leukemic cells and p16CDKN2A in Calu-6 lung carcinoma cells) and the inhibition of global DNA methylation in HL-60 leukemic cells increased with DAC concentration. (biomedcentral.com)
We now investigate whether inhibition of Akt signaling during ex vivo expansion of CAR T cells can promote the generation of CAR T cells with enhanced antitumor activity following adoptive therapy in a murine leukemia xenograft model. (biomedcentral.com)
Functionally, Akt inhibition did not dampen cell-mediated effector function, while Th1 cytokine production increased. (biomedcentral.com)
Inhibition of Akt signaling during ex vivo priming and expansion gives rise to CD19CAR T cell populations that display comparatively higher antitumor activity. (biomedcentral.com)

HOX B6 gene is only expressed in erythoid progenitor cells, which are the precursor to red blood cells used for transport of oxygen and carbon dioxide throughout the body. (wikipedia.org)
Moreover, the RUNX1 mutant protein was more stable than WT and increased HIF1-α and its target ID1 gene expression in ASXL1 mutant cells. (biomedcentral.com)
The reporter cell line shows the cellular endogenous activity of pluripotency gene Oct3/4 with green fluorescent protein (GFP) while also reporting mesoderm-specific gene, Brachyury, activity with DsRed fluorescent protein. (siu.edu)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)
Manufacturing CAR T cells is a process that involves activation and ex vivo culture with IL-2 to facilitate CAR gene transfer and to achieve a therapeutic number of T cells. (biomedcentral.com)

In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)
Genotoxic damage causes robust alterations to pathways associated with B cell activation and increased proliferation, suggesting that genotoxic damage initiates not only the normal B cell maturation processes but also mimics activated B cell response to antigenic agents. (biomedcentral.com)
The RAW264.7 cell line is most commonly used to study osteoclast differentiation and its signaling pathways. (sciforschenonline.org)

Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)

Leukemia and lymphoma are blood malignancies that affect people of all ages and result in approximately 23,000 deaths in the United States per year [ 1 ]. (nature.com)
Here we describe the phenotypic and molecular characteristics of the B cell leukemia/lymphoma in the Tg mice. (biomedcentral.com)
Expression of B cell-stimulatory cytokines IL-1β, IL-6, IL-10, IL-12 p40 , IL-13 and TNFα and HIV proteins p17, gp120 and nef were elevated in the Tg mice with lymphoma. (biomedcentral.com)
Increased expression of HIV proteins and the B-cell stimulatory factors is consistent with the interpretation that one or more of these factors play a role in lymphoma development. (biomedcentral.com)
Human Immunodeficiency Virus Type I (HIV-1) infection is associated with an elevated incidence of B-cell non-Hodgkin's lymphoma (NHL) and in recent years also with Hodgkin's lymphoma [ 1 ]. (biomedcentral.com)
the most common are diffuse large B-cell lymphoma and Burkitt's lymphoma [ 1 ]. (biomedcentral.com)
Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)
genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)

Rates of complete remission (CR), prognosis and survival depend on the grade of anemia, thrombocytopenia, white blood cell expansion and karyotypes. (nature.com)
The mouse cardiac function of cardiac angiogenesis was measured and cell survival of aged hBM-MSCs was investigated. (biomedcentral.com)
There is increasing evidence that T cells are able to control tumor growth and survival in cancer patients, both in early and late stages of the disease. (aacrjournals.org)
VEGF, produced in large amounts by cancer cells during tumor growth, interacts with its receptors VEGFR1 and VEGFR2 thereby causing endothelial cell survival, proliferation, and sprouting [7] . (plos.org)

HOXB6 has the ability to cause the indefinite proliferation of murine marrow cells, as well as expand hematopoietic stem cells. (wikipedia.org)
13 ] found that miR-10a and miR-10b were significantly increased in CD34 + marrow cells from 28 patients with myelodysplastic syndrome compared with healthy donors. (biomedcentral.com)

Monocyte differentiation required infectious VSV, but loss of CD14+ cells was also associated with the presence of a cytokine/chemokine milieu produced in response to VSV infection. (karger.com)
Since then it has become an important cell line to study monocyte differentiation. (sciforschenonline.org)

Likewise, bispecific antibodies for engagement of other cytotoxic immune cells were constructed, for instance, targeting Fcγ RI/CD64 on macrophages and Her2/neu or EGFR on tumor cells. (aacrjournals.org)
Therefore, we co-cultured AML cells with macrophages. (frontiersin.org)
Macrophages are the most abundant white blood cells in the immune microenvironment and have a high degree of heterogeneity. (frontiersin.org)
Although these protocols provide a successful method to culture osteoclasts from RAW264.7 cells, they don't express the issue of having multinucleated cells such as macrophages mixing with osteoclasts in the population. (sciforschenonline.org)
Cell-mediated response refers to the activation of macrophages, natural killer cells, and antigen-specific T-lymphocytes that attack anything perceived as a foreign agent. (livonlabs.com)

They can inhibit the growth of tumor cells, kill them via phagocytosis, and promote anti-tumor immunity. (frontiersin.org)
Anti-tumor activity was evaluated after adoptive transfer of the CD19CAR T cells into CD19+ tumor-bearing immunodeficient mice. (biomedcentral.com)

These Human bronchial epithelial cells expressing BMI-1 retain both viability and differentiation potential of wild-type human bronchial epithelium while importantly not demonstrating changes in cell karyotype. (cancertools.org)
Normal human bronchial epithelial cells were grown in a growth factor-supplemented medium and differentiated at ALI in bronchial epithelial differentiation medium. (cancertools.org)
Expression of polycomb protein BMI-1 maintains the plasticity of basal bronchial epithelial cells. (cancertools.org)

A leading contributing factor to metastasis and treatment resistance is the heterogeneity and plasticity of the cells within tumors 6 . (nature.com)
But nonviral tumors, which are composed of aberrant host cells, do not provide such targets, and the immune response is suppressed by defenses against autoimmune reactions. (the-scientist.com)
The role of two members of the ETS (E26 avian leukemia oncogene) family of transcription factors, ETS-1 and ETS-2, has been investigated in many cancers but has not yet been studied in ocular tumors. (molvis.org)
Targeted delivery of tumor necrosis factor-α to tumor vessels induces a therapeutic T cell-mediated immune response that protects the host against syngeneic tumors of different histologic origin. (southernbiotech.com)
Immune cells abundantly infiltrate tumors, creating a complex environment mediated by repetitive cycles of antitumor responses and immune evasion ( 1 ). (frontiersin.org)
In mice with L1210 leukemia and in mice bearing EMT6 tumors, the antineoplastic action of DAC also increased with the dose. (biomedcentral.com)
Here we report a phenotypic and molecular characterization of B cell tumors that develop in Tg26 mice. (biomedcentral.com)
Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)

Human Immunodeficiency Virus Type I (HIV-1) infection is associated with a high incidence of B-cell lymphomas. (biomedcentral.com)
1] Siegel B, "Enhanced interferon response to murine leukemia virus by ascorbic acid" Infection and Immunity 1974 10(2):409-410. (livonlabs.com)

Additional sex combs-like 1 ( ASXL1 ) mutations have been described in all forms of myeloid neoplasms including chronic myelomonocytic leukemia (CMML) and associated with inferior outcomes, yet the molecular pathogenesis of ASXL1 mutations ( ASXL1 -MT) remains poorly understood. (biomedcentral.com)
Previously, we observed that transcription factor RUNX1 mutations ( RUNX1 -MT) coexisted with ASXL1 -MT in CMML and at myeloid blast phase of chronic myeloid leukemia. (biomedcentral.com)
The contribution of RUNX1 mutations in the pathogenesis of myeloid transformation in ASXL1 -mutated leukemia, however, remains unclear. (biomedcentral.com)
Co-expression of two mutant genes increased myeloid stem cells in animal model, suggesting that cooperation of RUNX1 and ASXL1 mutations played a critical role in leukemia transformation. (biomedcentral.com)

A broader interest of our laboratory is to understand the molecular regulation of HSCs both from the perspectives of self-renewal and differentiation, and how these features become altered upon disease-causing or disease-associated perturbations. (lu.se)
To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)

We previously showed that VEGF signaling in primary endothelial cell cultures caused overexpression of VEGFR2 in a positive feed-forward loop, which is at least partly regulated by loss of miRNA-mediated control of VEGF receptor degradation. (plos.org)

The assessment of cell proliferation and migration was conducted using EdU proliferation, wound healing, and transwell assays. (bvsalud.org)

They don't often exclusively differentiate into osteoclast but also into other multinucleated cells as well including macrophage polykaryons. (sciforschenonline.org)
The murine macrophage cell line RAW264.7 was first established almost four decades ago [5,6]. (sciforschenonline.org)
It has been shown that multinucleated foreign body giant cells also express tartrateresistant acid phosphatase (TRAP) [14], hence, classical TRAP staining is unable to distinguish between macrophage polykaryons and osteoclasts. (sciforschenonline.org)

Our goal is to understand, at cellular and molecular levels, what controls HSCs and the fate of other immature blood cells. (lu.se)
This offers significant advantages for studying tumor pathogenesis and molecular mechanisms of oncogenesis caused by a single initiating oncogenic event introduced through the mouse germ line. (molvis.org)

For example, normal stroma, the connective material that supports the cells of a tissue, appears to inhibit cancer growth. (the-scientist.com)

Both miRNAs increased the clonogenic B-cell potential of Mll-AF4 + cells. (ashpublications.org)

Out of the 313 unique human cytokines evaluated, 11 were found to expand cell numbers ≥2-fold in a 7-day culture. (haematologica.org)
Cytokines are essential for the function and maintenance of cells, and altered cytokine levels influence not only leukemic cells, but also the normal HSC within the BM. (haematologica.org)
Pathogen clearance is mediated by mechanisms such as phagocytosis, respiratory burst with the production of ROS and RNS and release of bacteriostatic peptides, but also through the cell-extrinsic initiation of inflammation via the release of proinflammatory cytokines and chemokines ( 24 ). (frontiersin.org)
Cytokines are communication proteins released by certain white blood cells that transmit information to other cells, promoting the immune response. (livonlabs.com)

Incomplete silencing of transgenes is a concern as well, and reactivation of Myc or other oncogenes after differentiation has been linked to tumor formation in iPSC-derived and iPSC-transplanted mice. (addgene.org)

These are capable of connecting any kind of cytotoxic T cell to a cancer cell, independently of T-cell receptor specificity, costimulation, or peptide antigen presentation. (aacrjournals.org)
Insufficient persistence and effector function of chimeric antigen receptor (CAR)-redirected T cells have been challenging issues for adoptive T cell therapy. (biomedcentral.com)
Upon engagement of cell surface receptors such as the T cell receptor, co-stimulatory molecules, and cytokine receptors, the PI3K-Akt pathway is activated, resulting in downstream responses via phosphorylating a range of intracellular proteins. (biomedcentral.com)

Regularly, anergy of T cells or other immune cells can be regularly reverted to anti-leukemic functionality. (nature.com)
A pro-inflammatory environment is thought to provide a selective advantage for the leukemic stem cells (LSC). (haematologica.org)

Since it is a transcriptional factor, HOX B6 regulates erythpoigenesis (red blood cell formation) using mRNA as the basis for certain protein productions. (wikipedia.org)
Comparing these transcriptional responses provides a greater understanding of the mechanisms cells use in the differentiation between types of DNA damage and the potential consequences of different sources of damage. (biomedcentral.com)
Altogether, by identifying a pro-angiogenic VEGF/miR-101/EZH2 axis in endothelial cells we provide evidence for a functional link between growth factor-mediated signaling, post-transcriptional silencing, and histone-methylation in the angiogenesis process. (plos.org)

With so many deaths around the world being due to cardiovascular disease, there is great demand for an unlimited supply of cells for application in regenerative medicine. (siu.edu)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
Derivation of autologous induced pluripotent stem cells (iPSCs) through direct reprogramming of easily accessible somatic cells holds the potential to transform the field of regenerative medicine. (biomedcentral.com)

Passage 2 heterogeneous cells were plated in a 6-well plate at 5 x 10 4 cells per well and grown overnight. (cancertools.org)
Random integration also creates heterogeneous iPSC cell lines, which can complicate comparisons made between lines. (addgene.org)
AML is a highly heterogeneous hematological disease and is the second most common form of leukemia. (mdpi.com)

JOA displayed the activity of inhibiting the BCR-ABL and promoted differentiation of not only imatinib -sensitive but also imatinib -resistant cells with BCR-ABL mutation, which could become a potent lead compound to overcome the imatinib -resistant induced by inhibitors of BCR-ABL tyrosine kinase in CML therapy. (jcancer.org)
The DNMT3A mutation can cause significant changes in epigenetic modifications and is one of the essential regulatory factors for the occurrence and development of leukemia ( 4 ). (frontiersin.org)

The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)

Aberrant regulation of blood cell formation underlies a wide range of hematological disorders, including leukemia. (lu.se)

This process is initiated by RAG endonucleases while the lymphocytes are in the G1 phase of the cell cycle. (biomedcentral.com)
T-lymphocytes are a type of white blood cell. (livonlabs.com)
Natural killer cells are lymphocytes that can directly attack cells, like tumor cells, and kill them. (livonlabs.com)

Classification of adult MDS is based upon the French-American-British (FAB) classification of MDS (1982) and consists of five categories: (1) refractory anemia, (2) refractory anemia with ring sideroblasts (RARS), (3) RAEB, (4) RAEB-T, and (5) and myelomonocytic leukemia. (medscape.com)
Monosomy 7 is most often associated with juvenile myelomonocytic leukemia (JMML), and as many as 30% of children with JMML have a deletion of all or part of chromosome 7. (medscape.com)

To further promote differentiation and to increase mechanical signaling, Notch signaling was activated by presenting cells with fibronectin and DLL1 protein. (siu.edu)
The differentiation was found to have a pronounced effect in the presence of fibronectin and DLL1 protein together with the withdrawal of LIF. (siu.edu)
3 Detection of the MLL-AF4 fusion protein in CD34 + /CD19 − fetal hematopoietic stem or progenitor cells or in fetal cells before hematologic specification suggests that there is a window of opportunity during development for the MLL-AF4 fusion protein to immortalize hematopoietic stem and progenitor cells (HSPCs). (ashpublications.org)
In an earlier study, Barrett et al used a conditional invertor mouse line for cell-specific expression of an Mll-AF4 fusion protein. (ashpublications.org)

However, the control of directing pluripotent cells into mesoderm lineage which gives rise to cardiac muscle cells remains poor. (siu.edu)
This is a proof-of-concept that mechanical signaling together with synergistic chemical signaling can drive pluripotent cells towards mesoderm lineage. (siu.edu)
This requirement strongly limits the number of solutions or entiation and lineage-specification, programmed cell death, and ``states'' for the system. (lu.se)
2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)
To address the role of these miRNAs in transformation, the investigators overexpressed them in lineage-marker-depleted Sca1 + Kit + (LSK) fetal liver cells from targeted Mll-AF4 invertor mice (see figure). (ashpublications.org)

Additionally, lingering expression or re-expression of viral transgenes in iPSC-derived cells can interfere with their differentiation potential. (addgene.org)

Extracellular Vesicles (EVs) are membranous vesicles produced by all cells under physiological and pathological conditions. (nature.com)

Effective immune surveillance of patients with hematologic malignancies such as leukemia is mediated by cellular and noncellular arms of the innate and adaptive immune system. (nature.com)
Furthermore, other cells at the interface of the innate and the adaptive immune system (e.g cytokine-induced killer cells (CIK) or invariant natural killer T-cells (iNKT)) are important mediators in antitumor-, autoimmune-, and antimicrobial responses and tumor surveillance. (nature.com)
Over the past decade, cancer immunotherapy has been steering immune responses toward cancer cell eradication. (frontiersin.org)
However, tumor-specific T-cell responses are difficult to mount and sustain in cancer patients, and are limited by numerous immune escape mechanisms of tumor cells selected during immunoediting. (aacrjournals.org)
Vitamin C supports the cell-mediated immune system response. (livonlabs.com)
They are an integral part of the cell-mediated immune defense system. (livonlabs.com)
As mentioned in #7, these cells are essential to cell-mediated immune responses, and Vitamin C helps them to multiply in number. (livonlabs.com)
These white blood cells make antibodies as part of the antibody-mediated immune response. (livonlabs.com)
Some of the primary cells in the immune system concentrate Vitamin C as much as 80 times higher than the level in plasma. (livonlabs.com)

Anatomy8

Organisms13

Diseases19

Chemicals and Drugs13

Analytical, Diagnostic and Therapeutic Techniques and Equipment5

Phenomena and Processes16

Information Science3

Apoptosis14

Clonal7

Embryonic stem5

Moloney2

Monocytic4

Acute12

Monocytes5

Proliferation and differentiation3

Tumor cell5

Transgenic2

Lineages3

Stem cell16

Vitro9

Dendritic4

Bone11

Progenitor cell1

Terminal differentiation2

Senescence4

Cycle arrest2

Myeloid cells9

Assay1

Inhibition7

Gene6

Pathways3

Hematopoiesis1

Lymphoma8

Survival4

Marrow cells2

Monocyte2

Macrophages5

Anti-tumor2

Epithelial cells3

Tumors8

Infection2

Mutations4

Self-renewal2

Overexpression1

Assays1

Macrophage3

Molecular2

Inhibit1

Clonogenic1

Cytokines4

Oncogenes1

Receptor3

Leukemic2

Transcriptional3

Regenerative Medicine3

Heterogeneous3

Mutation2

Neonatal1

Hematological1

Lymphocytes3

Myelomonocytic2

Protein4

Lineage5

IPSC-derived1

Physiological1

Immune9