AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation Science
Cell DifferentiationLeukemiaLeukemia, MyeloidLeukemia, Myeloid, AcuteLeukemia, LymphoidGene Expression Regulation, LeukemicLeukemia, ExperimentalHL-60 CellsTumor Cells, CulturedCell DivisionCells, CulturedCell LineLeukemic InfiltrationBone MarrowHematopoietic Stem CellsTretinoinTranscription FactorsK562 CellsRNA, MessengerSignal TransductionLeukemia, Promyelocytic, AcuteStem CellsCell ProliferationApoptosisLeukemia, Monocytic, AcuteBone Marrow CellsFlow CytometryLeukemia, T-CellGranulocytesPrecursor Cell Lymphoblastic Leukemia-LymphomaGene Expression Regulation, DevelopmentalMolecular Sequence DataBase SequenceCell LineageGene Expression RegulationSex DifferentiationDNA-Binding ProteinsLeukemia-Lymphoma, Adult T-CellLeukemia, Erythroblastic, AcutePhenotypeU937 CellsLeukemia, Myelogenous, Chronic, BCR-ABL PositiveEmbryonic Stem CellsNeoplastic Stem CellsCell SurvivalReverse Transcriptase Polymerase Chain ReactionAntigens, DifferentiationNeoplasm ProteinsCytarabineClone CellsCell Line, TumorBlast CrisisGene ExpressionLeukemia, Lymphocytic, Chronic, B-CellCell CycleArabinofuranosylcytosine TriphosphateCell Transformation, NeoplasticDimethyl SulfoxideMice, Inbred C57BLAntigens, CDHomeodomain ProteinsTranscription, GeneticHematopoiesisB-LymphocytesOncogene Proteins, FusionProto-Oncogene ProteinsImmunophenotypingTransfectionT-LymphocytesAntineoplastic AgentsLeukemia L1210Leukemia, B-CellTime FactorsFusion Proteins, bcr-ablTetradecanoylphorbol AcetateMice, TransgenicColony-Forming Units AssayAcute DiseaseImmunohistochemistryDown-RegulationAntigens, SurfaceBlotting, WesternOsteogenesisLeukemia, Myelomonocytic, AcuteMesenchymal Stromal CellsOsteoblastsMonocytesMice, KnockoutTranslocation, GeneticGene Expression ProfilingMutationAntigens, CD34Antibodies, MonoclonalTrans-ActivatorsGranulocyte-Macrophage Colony-Stimulating FactorNuclear ProteinsLymphocytesAdipogenesisInterleukin-3Cell Culture Techniques
ApoptosisSelf-renewalProliferation and differentiationCytokinesMutationsGrowth and differentiationInduces differentiationVitroProgressionGranulocyticAcuteMonocyticLeukemiaMonocytesInhibitionMyeloid cellMalignantEmbryonic StemInductionMesenchymal stemPathwaysPhysiologicStemnessFLT3AberrantAntigenAbnormalInfiltrationBoneTherapiesMyelomonocyticTumor cellAssaysMechanismsStem Cell TransplantInhibitorRetinoic acidTherapyMolecular BiologyInterestinglyProgenitorsMarkersVivoFlow cytometrySurvivalGeneAbstractProcessesPlasmacytoid dendritic celImmatureOutcomesMechanisticFate
Apoptosis10
  • Induction of apoptosis, cell-cycle analysis, levels of specific membrane differentiation markers were evaluated by flow cytometry. (unibo.it)
  • More than 15 years ago, cholesterol decrement was first shown to inhibit tumor cell growth, metastasis of tumor cells, and induction of apoptosis (7). (cdc.gov)
  • Several years later, Kerr and co-workers 2 defined the morphological changes in cells undergoing this process, also known as apoptosis. (nature.com)
  • We discuss here the contribution of glycan-lectin interactions to the initiation, execution and resolution of apoptosis and their emerging roles in other cell death programs including autophagy. (nature.com)
  • A successful therapy must therefore eliminate these cells known to be highly resistant to apoptosis. (hindawi.com)
  • In this paper, we describe the metabolic changes as well as the mechanisms of resistance to apoptosis occurring in cancer cells and cancer stem cells, underlying the connection between these two processes. (hindawi.com)
  • Chronic myeloid leukemia (CML) results from BCR-ABL oncogene, which blocks CML cells differentiation and protects these cells from apoptosis. (jcancer.org)
  • Here, we determined the effect of Jiyuan oridonin A (JOA), an ent -kaurene diterpenoid compound, on the differentiation blockade in imatinib-sensitive, particularly, imatinib-resistant CML cells with BCR-ABL-T315I mutation by cell proliferation assay, apoptosis analysis, cell differentiation analysis, cell cycle analysis and colony formation assay. (jcancer.org)
  • Abnormal proliferation, clonal expansion, aberrant differentiation, and diminished apoptosis (programmed cell death) lead to replacement of normal blood elements with malignant cells. (msdmanuals.com)
  • Second, the rapid proliferation of these cells, along with a reduction in their ability to undergo programmed cell death (apoptosis), results in their accumulation in the bone marrow, the blood, and, frequently, the spleen and liver. (medscape.com)
Self-renewal10
  • Autophagy is an intracellular degradation system that ensures a dynamic recycling of a variety of building blocks required for self-renewal, homeostasis, and cell survival under stress. (hindawi.com)
  • It is a heterogeneous disease, cellularly and molecularly, with an overarching theme of ectopic self-renewal and arrested differentiation potential at multiple levels of the hematopoietic tree ( 1 ). (frontiersin.org)
  • Akin to the normal hematopoietic system, leukemias are sustained by a small number of leukemia stem-like cells (LSC), which can be distinct from the normal hematopoietic stem cells (HSC) but also exhibit functional characteristics of self-renewal and (abnormal or hindered) differentiation, and are often quiescent ( 2 - 4 ). (frontiersin.org)
  • Emergence of LSC is dependent on individual or combined genetic mutations that broadly determine the cellular affiliation of the leukemia, and permit or impose ectopic self-renewal and a restricted differentiation potential into what constitutes the proliferative bulk of the leukemia ( 5 , 6 ). (frontiersin.org)
  • Hematopoiesis depends on a tightly controlled balance of self-renewal, proliferation, cell death and differentiation. (nature.com)
  • Acute myeloid leukemia (AML) is generally regarded as a stem cell disease, and it originates from a hierarchy of leukemic stem cell classes that differ in self-renewal capacity [ 1 , 2 ]. (researchsquare.com)
  • Melinda is highly proficient in the use of in vitro and in vivo assays for normal and malignant hematopoietic stem and progenitor cells, determination of self-renewal, expansion and lineage specific differentiation capabilities, and the use of induced pluripotent stem cells for the investigation of hematopoietic disorders and leukemic progression and the testing of targeted therapeutics. (edu.au)
  • Research has shown that lysine-specific histone demethylase 1 (LSD1) plays an essential role in the self-renewal of these "leukemic stem cells," Incyte principal investigator Sang Hyun Lee, Ph.D., tells GEN . Building on this finding, Dr. Lee and colleagues developed an LSD1 inhibitor called INCB059872, which spurs myeloid cell differentiation. (genengnews.com)
  • It is widely accepted at this time that leukemia is a disease of hematopoietic cells, occurring when these cells become capable of independent self-renewal irrespective of physiologic needs. (pickpdfs.com)
  • Malignant transformation usually occurs at the pluripotent stem cell level, although it sometimes involves a committed stem cell with more limited capacity for self-renewal. (msdmanuals.com)
Proliferation and differentiation3
  • Furthermore, the study examined the effects of cooperating oncoproteins such as Ras or Bcr/Abl, which allowed for both factor-independent proliferation and differentiation, or Bcl-2, which permitted factor-independent survival but not proliferation. (nih.gov)
  • It maintains normal hematopoietic function by interacting with hematopoietic stem cells (HSCs) to control their proliferation and differentiation ( Morrison and Scadden, 2014 ). (techscience.com)
  • 1 Neural stem/progenitor cell proliferation and differentiation: role of sonic hedgehog and wingless/int-1 proteins. (vinastemcelllab.com)
Cytokines7
  • A major limitation in approaching this problem has been the lack of suitable cell lines that exhibit normal, terminal, and synchronous differentiation in the absence of endogenous oncoproteins and in response to physiologic cytokines, and whose differentiation can be arrested by ectopically expressed human oncoproteins. (nih.gov)
  • Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
  • Indeed, natural killer (NK) and lymphocyte-activated killer (LAK) cell activation has been demonstrated in vivo and in vitro, and recent genetic studies reveal increased expression of key immune cytokines in response to treatment with PSK. (nih.gov)
  • PSK has also been shown to cause differentiation of leukemic cells in vitro, and this effect has been attributed to induction of differentiation cytokines. (nih.gov)
  • The bone marrow microenvironment (BMME) is a complex network system primarily comprising stromal cells such as mesenchymal stem cells (MSCs), osteoprogenitor cells, vascular endothelial cells, monocytes, macrophages, and cytokines. (techscience.com)
  • Co-Treatment of Prostate Cancer Cells with Mscs Expressing Trail and Iap Inhibitors Reduce Trail Resistance and Production of Trail-Induced Cytokines. (essex.ac.uk)
  • Indeed, cytokines released by leukemic B cells impair osteoblast differentiation and enhance osteoclast formation in vitro. (monocyte.eu)
Mutations6
  • and it is generally not aggressive until the tumor cells sustain mutations that block differentiation. (science20.com)
  • However, cancer cells overcome these controls, in particular by acquiring genetic mutations leading to the activation of oncogenes (pten, myc) or loss of tumor suppressors (p53) [ 1 ]. (hindawi.com)
  • The precise pathogenetic events leading to the development of ALL are still unknown, but evidence supports the hypothesis of driver mutations followed by secondary events, that can occur in subclones of the original leukemic cell following different evolution patterns ( 5 , 6 ). (frontiersin.org)
  • Acute myeloid leukemia results from the accumulation of mutations that deregulate survival and differentiation of hematopoietic cells, and drive leukemia. (alexslemonade.org)
  • The Paralkar Lab is interested in gaining a detailed mechanistic understanding of how chromatin proteins regulate the stemness-differentiation balance, and how mutations in them produce malignancy. (upenn.edu)
  • First, we screened 513 natural compounds on primary AML cells and identified a novel diterpene (H4) that preferentially induced differentiation of FLT3 wild-type AMLs, while FLT3-ITD/mutations conferred resistance. (lu.se)
Growth and differentiation1
  • SOS-1 is a guanine nucleotide-exchange factor that functions in the transduction of signals that control cell growth and differentiation 9,10 . (bvsalud.org)
Induces differentiation2
  • This substance induces differentiation and function of leukemic and other malignant cells. (elsevierpure.com)
  • By combinatorial screening using H4 and molecules with defined targets, we demonstrated that H4 induces differentiation by the activation of protein kinase C (PKC) signaling pathway, and in line with this, activates PKC phosphorylation and translocation of PKC to the cell membrane. (lu.se)
Vitro2
  • Transfection of MDS-MSCs with the miR-103-3p mimic improved osteogenic differentiation and decreased adipogenic differentiation in vitro, while inhibition of miR-103-3p showed the opposite results in NOR-MSCs. (techscience.com)
  • In a drop of blood we can identify all major blood cells and characterize their pathological changes in several disease conditions in vitro and in patient samples. (elifesciences.org)
Progression7
  • RESULTS: Starting from the concentration of 0.31 mg/ml, Hemidesmus indicus induced cytotoxicity and altered cell-cycle progression, through a block in the G0/G1 phase. (unibo.it)
  • While the importance of autophagy for cell homeostasis and survival has long been appreciated, its role in tumorigenesis and cancer progression is still developing [ 10 , 11 ]. (hindawi.com)
  • Bone marrow loss and a high proclivity to leukemic progression are the characteristics of myelodysplastic syndrome (MDS). (techscience.com)
  • Accumulating evidence shows that cancer stem cells are key drivers of tumor formation, progression, and recurrence. (hindawi.com)
  • Leukemia stem cells (LSCs) in acute myeloid leukemia (AML) played important roles in development of leukemia, chemotherapeutic drug resistance, and disease relapse and progression. (researchsquare.com)
  • Some persons with clonal hematopoiesis are at increased risk for the development of myeloid cancers such as acute myeloid leukemia or myelodysplastic syndromes, a risk that increases as the hematopoietic clone expands in size.16 Stopping this expansion may delay or avert leukemic progression, and therapeutic approaches to this end are being developed and tested. (cdc.gov)
  • Importantly, the proteomic make-up of blood stem cells and progenitors can regulate their cell fate and susceptibility to initiation as well as progression of leukemia. (lu.se)
Granulocytic3
  • Clones were identified that proliferated as immortalized blasts in the presence of estrogen, and that exhibited granulocytic, monocytic, or bipotential (granulocytic and monocytic) differentiation on estrogen withdrawal. (nih.gov)
  • Our data clearly suggest that granulocytic AML differentiation relies on noncanonical autophagy pathways and that restoring autophagic activity might be beneficial in differentiation therapies. (hindawi.com)
  • Myeloid sarcoma (MS), also known as granulocytic sarcoma or chloroma, is a rare extramedullary tumor composed of immature myeloid cells at different stages of differentiation which can involve any site of the body. (haematologica.org)
Acute7
  • We used primary acute myeloid leukemia (AML) samples and human AML cell lines to investigate the regulatory mechanisms of autophagy and its role in AML differentiation. (hindawi.com)
  • The groups study present evidence of myeloid neoplasms derived from patients with acute leukemias which encompass a spectrum of PDC differentiation which result in outcomes that do not fit into the categories of MPD MN or BPDCN. (news-medical.net)
  • The group concluded by stating that acute leukemia with PDC differentiation and typical cases of acute myeloid leukemia, NOS, or acute myelomonocytic leukemia are indistinguishable. (news-medical.net)
  • Myeloproliferative diseases are a heterogeneous group of disorders characterized by cellular proliferation of one or more hematologic cell lines in the peripheral blood, distinct from acute leukemia. (medscape.com)
  • High-throughput sequencing (HTS) of the immunoglobulin heavy chain ( IgH ) locus is a recent very efficient technique to monitor minimal residual disease of B-cell precursor acute lymphoblastic leukemia (BCP-ALL). (frontiersin.org)
  • B-cell precursor acute lymphoblastic leukemia (BCP-ALL) is the most common pediatric neoplasm ( 1 , 2 ). (frontiersin.org)
  • In acute myeloid leukemia (AML), malignant transformation and uncontrolled proliferation of an abnormally differentiated, long-lived myeloid progenitor cell results in high circulating numbers of immature blood cells and replacement of normal marrow by malignant cells. (msdmanuals.com)
Monocytic4
  • PDC progenitors are in common with monocytic and myeloid cell progenitors. (news-medical.net)
  • Beck's team further determined that the differentiation of PDC's may occur in the presence of monocytic or myeloid DC differentiation, or as a differentiation lineage possessing a predominant myoblast population. (news-medical.net)
  • This molecule is expressed by monocytic/myeloid lineage cells. (fishersci.com)
  • Furthermore, the combinatorial screening identified a bromo- and extra-terminal domain (BET) inhibitor that could further improve H4-dependent leukemic differentiation in FLT3 wild-type monocytic AML. (lu.se)
Leukemia13
  • The aim of this study was to investigate the cytodifferentiative, cytostatic and cytotoxic potential of a decoction of Hemidesmus indicus's roots (0.31-3 mg/mL) on a human promyelocytic leukemia cell line (HL-60). (unibo.it)
  • Therefore, our results suggest that CD45 dim CD34 + CD38 - CD133 + cells in AML might have the potential of leukemia stem cells. (researchsquare.com)
  • Chronic myeloid leukemia (CML) is a clonal expansion of the progenitor hematopoietic stem cells arising from the existence of the fusion BCR-ABL oncogene, that represents 15%-20% of the newly diagnosed cases of leukemia patients [ 1 - 2 ]. (jcancer.org)
  • Hematopoietic stem cell transplantation can be considered in young patients with chronic myelogenous leukemia in chronic phase if a human leukocyte antigen (HLA)-matched donor is available. (medscape.com)
  • That helps to eradicate the cells that are at the root of the leukemia, something conventional therapies can't do, Dr. Lee says. (genengnews.com)
  • In response to DNA damage, myeloid leukemia cells activate a poorly understood terminal differentiation process. (psu.edu)
  • Here, we show that the NFκB pathway directly activates expression of the proliferation inhibitor p21 in response to DNA damage in myeloid leukemia cells. (psu.edu)
  • show that this approach can detect characteristic changes that affect blood cells as a result of malaria, spherocytosis, bacterial and viral infections, and leukemia. (elifesciences.org)
  • In this application we are proposing studies in mice and leukemic cells to test the role of BIM inhibition as a target for leukemia, and to test RAS inhibitors as candidate therapies for these leukemias. (alexslemonade.org)
  • Research in the Paralkar Lab spans the spectrum from human patient sample studies and mouse models to cutting-edge molecular biology tools, high-throughput sequencing approaches, and novel computational algorithms, all with the goal of gaining insight into how the transcription of coding genes and noncoding ribosomal DNA genes is regulated in hematopoietic stem cells, myeloid progenitors, and in leukemia. (upenn.edu)
  • The Paralkar Lab has identified that key hematopoietic and leukemic transcription factors bind to rDNA and regulate rRNA transcription, and we are interested in understanding how the binding of cell-type-specific transcription factors regulates the activity of Polymerase I and the transcription of rRNA in normal hematopoiesis, and how this regulation is co-opted in leukemia to drive abundant ribosome biogenesis. (upenn.edu)
  • A High Percentage of CD16+ Monocytes Correlates with the Extent of Bone Erosion in Chronic Lymphocytic Leukemia Patients: The Impact of Leukemic B Cells in Monocyte Differentiation and Osteoclast Maturation. (monocyte.eu)
  • This enabled identification of TFs that can be uniquely associated to the tumor cells of chronic lymphocytic leukemia (CLL), diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL), hairy cell leukemia (HCL), and mantle cell lymphoma (MCL). (lu.se)
Monocytes4
  • Leukemic myoblast differentiation into PDCs occurs as a result of sharing a common hematopoietic precursor to dendritic cells, monocytes, and myeloid cells. (news-medical.net)
  • It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation. (fishersci.com)
  • CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. (thermofisher.com)
  • Interestingly, the evidence that monocytes, polarized toward the M2 phenotype, were characterized by high CD16 expression and showed a striking propensity to differentiate toward osteoclasts may provide further explanations for the enhanced levels of bone erosion detected, in agreement with the high number of immunosuppressive-M2 cells present in these patients. (monocyte.eu)
Inhibition2
  • We found a significantly lower expression of key autophagy- (ATG-) related genes in primary AML as compared to healthy granulocytes, an increased autophagic activity during all- trans retinoic acid- (ATRA-) induced neutrophil differentiation, and an impaired AML differentiation upon inhibition of ATG3, ATG4D, and ATG5. (hindawi.com)
  • Mechanistically, the cell differentiation mediated by JOA may be originated from the inhibition of BCR-ABL/c-MYC signaling in CML cells expressing wild-type BCR-ABL and BCR-ABL-T315I. (jcancer.org)
Myeloid cell2
  • This report describes clonal, granulocyte-macrophage colony-stimulating factor-dependent myeloid cell lines that exhibit these properties. (nih.gov)
  • These myeloid cell lines provide tools for examining the biochemical and genetic pathways that accompany normal differentiation as well as a system in which to dissect how other leukemic oncoproteins interfere with these pathways. (nih.gov)
Malignant3
  • The Paralkar Lab has developed customized genomes and computational pipelines to map datasets to rDNA, and we are interested in developing advanced tools to map and interpret the genetic and epigenetic profiles of rDNA in normal and malignant cells. (upenn.edu)
  • 20 Malignant gliomas: treatment using genetically-modified neural stem cells. (vinastemcelllab.com)
  • POT1 mutation carriers had a range of benign and malignant neoplasms involving epithelial, mesenchymal, and neuronal tissues in addition to B- and T-cell lymphoma and myeloid cancers. (cdc.gov)
Embryonic Stem1
Induction2
  • More rarely, myeloid sarcoma could also be observed in the setting of myelodysplastic syndrome or myeloproliferative neoplasms, or as an isolated myeloid sarcoma (also designated as de novo , non-leukemic or primary MS). 1 Common practice suggests that patients with isolated myeloid sarcoma should receive AML-like induction chemotherapy. (haematologica.org)
  • Treatment includes induction chemotherapy to achieve remission and postremission chemotherapy (with or without stem cell transplantation) to avoid relapse. (msdmanuals.com)
Mesenchymal stem7
Pathways5
  • The molecular pathways of normal myeloid differentiation, as well as the mechanisms by which oncogenes disrupt this process, remain poorly understood. (nih.gov)
  • 10 Galectins can function inside the cells by modulating signaling pathways, 11 although they also act extracellularly by establishing multivalent interactions with cell surface glycans and delivering signals that lead to disruption of cellular homeostasis. (nature.com)
  • Lipids, amino acids, and nucleotides necessary for the biosynthesis of the daughter cells are mostly provided by intermediate metabolites of these pathways. (hindawi.com)
  • To prevent aberrant cell proliferation, these pathways are tightly regulated. (hindawi.com)
  • Stimulation of T cells through the T-cell receptor results in the activation of a series of signaling pathways that leads to the secretion of interleukin (IL)-2 and cell proliferation. (cdc.gov)
Physiologic2
  • Herein, we review the role of glycans and glycan-binding proteins as essential components of the cell death machinery during physiologic and pathologic settings. (nature.com)
  • Is there a hallmark 'glycosylation signature' that characterizes the initiation, execution and resolution of cell death programs in physiologic and pathologic settings? (nature.com)
Stemness1
FLT31
  • Both AG-221 and 5-Azacytidine cooperated with a FLT3 inhibitor to reverse aberrant hypermethylation and suppress leukemic stem cells. (aacrjournals.org)
Aberrant1
  • In a related study, Shih, Meydan, and colleagues showed that AG-221 reversed aberrant DNA hypermethylation and induced differentiation in IDH2 R140Q AML cells, and the DNA hypomethylating agent 5-Azacytidine had similar effects in TET2 -mutant AML. (aacrjournals.org)
Antigen2
  • PDCs are a class of specialized dendritic cells that play a role in immunomodulatory functions and antigen presentation. (news-medical.net)
  • [ 2 ] Options for second-line therapy in patients with relapsed/refractory disease include chemotherapy-free regimens with biologic targeted agents such as covalent Bruton tyrosine kinase (BTK) inhibitors, lenalidomide,venetoclax, and chimeric antigen receptor (CAR) T-cell therapy. (medscape.com)
Abnormal1
  • Toward this end, an insertional transgenic mouse mutant has been isolated that results in abnormal germ-cell development. (shengsci.com)
Infiltration2
  • symptoms of extramedullary leukemic infiltration are present in only about 5% of patients (often as skin manifestations). (msdmanuals.com)
  • Patients with AML present with symptoms resulting from bone marrow failure, symptoms resulting from organ infiltration with leukemic cells, or both. (medscape.com)
Bone12
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • The group analyzed seven MN patients who presented with PDC proliferations comprising 5-26% of that blood cell or bone marrow. (news-medical.net)
  • The bone marrow stromal cell (BMSC) population, which makes up a large part of the hematopoietic microenvironment, encompasses a variety of adherent cell types. (techscience.com)
  • We examined the CD45 dim CD34 + CD38 - CD133 + cells on bone marrow samples of hematologic malignancies and healthy controls using four-color flow cytometry experiments. (researchsquare.com)
  • Interestingly, the CD45 dim CD34 + CD38 - CD133 + cells were highly expressed in bone marrow of patients with AML compared to that of healthy controls (HC). (researchsquare.com)
  • In this study, we focused on measuring LSCs easily in the bone marrow cells from AML patients by developing a four-color flow cytometric analysis. (researchsquare.com)
  • Establish stromal cell layers and culture hematopoietic progenitors in "Dexter-type" long-term bone marrow cultures. (stemcell.com)
  • Normal hematopoiesis requires an intricate balance in the bone marrow between the ability of stem cells to maintain themselves for decades of life while producing billions of mature blood cells every day. (upenn.edu)
  • Moreover, the partial colonization of the bone with reactive bone marrow we documented via PET-FDG imaging suggests that neoplastic cell overgrowth contributes to bone derangement. (monocyte.eu)
  • Following treatment, serum calcium and phosphate levels were measured, bone structure and mineral density were measured by microcomputed tomography, and bone cells measured by static and dynamic histomorphometry. (bvsalud.org)
  • Diabetic mice were transplanted with HSCs and insulin-producing cells (IPCs) via the tail vein after destroying the bone marrow. (vinastemcelllab.com)
  • The underlying pathophysiology in AML consists of a maturational arrest of bone marrow cells in the earliest stages of development. (medscape.com)
Therapies2
  • hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
  • 21 The cancer stem cell hypothesis and its impact on the design of new cancer therapies. (vinastemcelllab.com)
Myelomonocytic3
  • Siglec F is expressed mostly on eosinophils and alveolar macrophages and lower levels of this receptor have also been reported on immature myelomonocytic cells. (fishersci.com)
  • Probably controls the inflammatory response in myelomonocytic cells at the level of the cytoskeleton organization. (lu.se)
  • Expression increases throughout myelocyte differentiation and peaks in the mature myelomonocytic cells. (lu.se)
Tumor cell3
  • Studies have suggested that statins may inhibit tumor cell growth and possibly prevent carcinogenesis. (cdc.gov)
  • Cytotoxicity of extracts and compounds isolated from Croton echioides in animal tumor cell (HTC). (scielo.br)
  • However, most GEP studies have typically been performed on whole tissue samples, containing varying degrees of tumor cell content, which results in uncertainties in data analysis. (lu.se)
Assays1
  • Differentiation was normal and terminal as evidenced by morphology, cell surface markers, gene expression, and functional assays. (nih.gov)
Mechanisms2
  • Two decades later, pioneering studies suggested that lectin-like molecules constitutively expressed on the surface of macrophages can selectively recognize changes on glycans decorating the surface of apoptotic thymocytes, 4 , 5 although these studies likewise did not provide substantial insight into the mechanisms by which lectin-glycan interactions regulate cell death. (nature.com)
  • Based on the pathophysiology of diabetes mellitus, stem cell therapy targets two mechanisms, namely cell replacement and immune correction. (vinastemcelllab.com)
Stem Cell Transplant3
  • Recently, we and others have shown that allogeneic hematopoietic stem cell transplantation (allo-HSCT) may represent a valid treatment option in leukemic myeloid sarcoma. (haematologica.org)
  • 25 Severe combined immunodefieciency patients: immune recovery after stem cell transplantation. (vinastemcelllab.com)
  • Treatment selection takes into account patient age, fitness, and whether autologous stem cell transplantation (ASCT) is planned. (medscape.com)
Inhibitor2
  • Yen and colleagues generated a potent allosteric inhibitor of IDH2 R140Q , AG-221, which reduced 2HG production, promoted differentiation of AML blasts, and extended survival in AML xenografts. (aacrjournals.org)
  • Preclinical tests show that the inhibitor can change the microenvironment of a tumor by increasing populations of antitumor immune cells in the area. (genengnews.com)
Retinoic acid1
  • In the presence of retinoic acid, RARA is activated and terminal differentiation of promyelocytes occurs. (medscape.com)
Therapy7
  • This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)
  • JOA displayed the activity of inhibiting the BCR-ABL and promoted differentiation of not only imatinib -sensitive but also imatinib -resistant cells with BCR-ABL mutation, which could become a potent lead compound to overcome the imatinib -resistant induced by inhibitors of BCR-ABL tyrosine kinase in CML therapy. (jcancer.org)
  • Trang chủ Công trình KHCN Sách/Chương sách Improving the Efficacy of Diabetes Mellitus Treatment by Combining Cell Replacement Therapy. (vinastemcelllab.com)
  • Stem cell therapy has the potential to overcome these limitations and may offer the best outcomes for treating diabetes mellitus. (vinastemcelllab.com)
  • The aim of this series of studies was to evaluate the efficiency of diabetic treatment by combining cell replacement therapy with immune correction therapy. (vinastemcelllab.com)
  • These findings provide hope for a new strategy that can improve the outcomes of stem cell-based therapy for diabetes in humans. (vinastemcelllab.com)
  • 18 Adipose-derived stem cells: therapy through paracrine actions. (vinastemcelllab.com)
Molecular Biology1
  • Indeed, with modern cellular and molecular biology techniques, a better understanding of the specific molecular effects of PSK on tumor cells as well as leukocytes may be determined. (nih.gov)
Interestingly1
  • Interestingly, studies have also shown that PSK may actually inhibit carcinogenesis by inhibiting the action of various carcinogens on vulnerable cell lines. (nih.gov)
Progenitors3
  • The cell lines were established by conditional immortalization of primary murine marrow progenitors with an estrogen-regulated E2a/Pbx1-estrogen receptor fusion protein. (nih.gov)
  • In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)
  • We have examined the effects of a stromal cell-derived cytokine designated interleukin 11 (IL-11) on the proliferation of murine hemopoietic progenitors in methylcellulose culture. (shengsci.com)
Markers2
  • They examined multiple markers of cell identity (CD22, CD123, CD303, and CD304) to determine the subgroups of PDCs. (news-medical.net)
  • 17 Human hair follical stem cells: markers, selection and perspective clinic application. (vinastemcelllab.com)
Vivo1
Flow cytometry3
  • Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. (bdbiosciences.com)
  • GEP analyses were performed on highly purified, flow-cytometry sorted tumor-cells from eight subgroups of BCLs. (lu.se)
  • In this study, highly purified, flow-cytometry sorted, classified in relation to normal B-cell differentiation [1]. (lu.se)
Survival5
  • Basal macroautophagy (thereafter referred to as autophagy), a catabolic recycling system in cells, is key to maintaining cellular homeostasis and survival. (hindawi.com)
  • Furthermore, activation of autophagy allows to extend cell survival when exposed to different types of stressors such as starvation or cytotoxic drugs. (hindawi.com)
  • Understanding the function of lectin-glycan recognition systems in cell death will facilitate the implementation of novel therapeutic strategies aimed at controlling unbalanced cell proliferation and survival in several pathologic conditions. (nature.com)
  • Additionally, the high levels of the CD45 dim CD34 + CD38 - CD133 + cells in AML patients were an independently significant poor risk factor for overall survival and event free survivals. (researchsquare.com)
  • Also, these cell populations directly correlated with poor survival [ 2 , 5 ]. (researchsquare.com)
Gene4
  • It is mainly expressed in hematopoietic cells and has an important role in regulating gene expression. (medscape.com)
  • 2 Sensitivity of hematopoietic and leukemic stem cells to hoxa gene levels. (vinastemcelllab.com)
  • Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repres- sion and transcriptional activation. (lu.se)
  • Increasing evidence emphasize that a substantial variance of protein expression is conveyed at the levels of protein synthesis and degradation, and that post-transcriptional control of gene expression plays a critical role in multiple aspects of cell biology, including coordination of cellular processes and rapid alteration of cellular phenotype. (lu.se)
Abstract1
  • abstract = "DNA damage exposure is a major modifier of cell fate in both normal and cancer tissues. (psu.edu)
Processes5
  • These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
  • Although one typically thinks of carbohydrates as associated with cell growth and viability, glycosylation also has an integral role in many processes leading to cell death. (nature.com)
  • Glycans, either alone or complexed with glycan-binding proteins, can deliver intracellular signals or control extracellular processes that promote initiation, execution and resolution of cell death programs. (nature.com)
  • Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. (thermofisher.com)
  • Most leukemic processes are believed to be initiated at the level of stem cells. (pickpdfs.com)
Plasmacytoid dendritic cel1
  • This work builds on existing knowledge that PDCs can be divided into two categories: mature PDC proliferations which result in myeloid neoplasms (MPDMN) and blastic plasmacytoid dendritic cell neoplasm (BPDCN). (news-medical.net)
Immature2
  • This disproves the scientific principle that fully formed, mature nerves cannot multiply like young, immature cells, Dyer said. (science20.com)
  • This family of related genes is critical to the ability of an immature cell to stop dividing and begin to differentiate so it acquires certain specific characteristics required to do its job in the body. (science20.com)
Outcomes3
  • Comparison of outcomes between isolated myeloid sarcoma and leukemic myeloid sarcoma. (haematologica.org)
  • Overall results and comparison of outcomes between isolated and leukemic myeloid sarcoma are given in Table 2 . (haematologica.org)
  • 8 As outcomes of isolated and leukemic myeloid sarcoma were found to be similar, data for the two groups were pooled to perform univariate and multivariate analyses. (haematologica.org)
Mechanistic1
  • Future work will include detailed proteomic profiling of ontogeny-specific leukemic features, investigation of the mechanistic details of hematopoietic stem cell plasticity, as well as specific types of post-translational modifications of particular importance in early hematopoiesis. (lu.se)
Fate4
  • In normal cells, the fate of pyruvate depends on many factors, one of which is oxygen availability. (hindawi.com)
  • DNA damage exposure is a major modifier of cell fate in both normal and cancer tissues. (psu.edu)
  • Our results uncover a p53-independent pathway for p21 activation involved in controlling hematopoietic cell fate. (psu.edu)
  • The rate of ribosome production has far-reaching influence on the fate of the cell, and dictates its size, proliferation, and ability to translate global or specific mRNAs. (upenn.edu)