Differentiation into myotubes regulates satellite cell differentiation skeletal muscle proliferation and differentiation. Medical search. Frequent questions

Skeletal muscle regeneration is regulated by coordinated activation of multiple signaling pathways. (elifesciences.org)
However, the role of individual arms of the UPR in skeletal muscle regeneration remain less understood. (elifesciences.org)
Myofiber-specific ablation of IRE1α or XBP1 in mice diminishes skeletal muscle regeneration that is accompanied with reduced number of satellite cells. (elifesciences.org)
Finally, targeted ablation of IRE1α also reduces Notch signaling, abundance of satellite cells, and skeletal muscle regeneration in the mdx mice, a model of Duchenne muscular dystrophy. (elifesciences.org)
Collectively, our experiments suggest that the IRE1α-mediated signaling promotes muscle regeneration through augmenting the proliferation of satellite cells in a cell non-autonomous manner. (elifesciences.org)
The findings shed new light on mechanisms of regeneration of healthy myofibers after severe tissue injury and suggest interplay between these fibers and muscle satellite cells though mechanisms remain to be elucidated. (elifesciences.org)
However, the mechanisms of muscle regeneration are not yet fully understood. (elifesciences.org)
These cells represent the oldest known adult stem cell niche, and are involved in the normal growth of muscle, as well as regeneration following injury or disease. (wikipedia.org)
The process of muscle regeneration involves considerable remodeling of extracellular matrix and, where extensive damage occurs, is incomplete. (wikipedia.org)
Remarkably type 2 cytokine signaling is also required in FAPs but not myeloid cells for quick clearance of necrotic debris a process that is necessary for timely and total regeneration of BV-6 cells. (scienceexhibitions.org)
2008 In addition to satellite cells recent studies have recognized an important part for fibro/adipogenic progenitors (FAPs) in muscle mass regeneration and its fatty degeneration (Joe et al. (scienceexhibitions.org)
In a number of species cells regeneration is associated with the presence of the molecular signature for type 2 innate immune response such as alternatively triggered (M2) macrophages and eosinophils (Allen and Wynn 2011 Palm et al. (scienceexhibitions.org)
Muscle satellite cells (MuSCs), myogenic stem cells in skeletal muscles, play an essential role in muscle regeneration. (bvsalud.org)
Our genetic engineering approach in mice revealed that PIEZO1 is functionally expressed in MuSCs and that Piezo1 deletion in these cells delays myofibre regeneration after injury. (bvsalud.org)
Since one of the mechanisms of muscle wasting in cachexia is defective muscle regeneration due to impaired myogenic differentiation, we examined the effect of IL11 on the differentiation of C2C12 mouse myoblasts. (bvsalud.org)
Our team is working on the molecular and cellular actors involved in human muscle regeneration , in muscle ageing and in muscular dystrophies including oculopharyngeal muscular dystrophy (OPMD) and Duchenne muscular dystrophy (DMD). (institut-myologie.org)
More precisely we are working on RNA metabolism , muscle regeneration , muscle stem cells , and fibrosis , with the final aim of developing innovative therapeutic approaches. (institut-myologie.org)
Low-intensity pulsed ultrasound (LIPUS, a form of mechanical stimulation) can promote skeletal muscle functional repair, but a lack of mechanistic understanding of its relationship and tissue regeneration limits progress in this field. (ijbs.com)
We investigated the hypothesis that specific energy levels of LIPUS mediates skeletal muscle regeneration by modulating the inflammatory microenvironment. (ijbs.com)
LIPUS at intensity of 60mW/cm 2 could significantly promoted skeletal muscle regeneration through shifting macrophage phenotype from M1 to M2. (ijbs.com)
Skeletal muscle growth and regeneration involves the activity of resident adult stem cells, namely satellite cells (SC). (sdbonline.org)
Collectively, this study provides the first evidence that transient levels of the evolutionarily conserved factor RACK1 are critical for adult SC activation and proper skeletal muscle regeneration, favoring the efficient progression of SC from a committed to a fully differentiated state. (sdbonline.org)
In 2009 beginning of 2010 we have focused on investigating what factors human embryonic stem cells (hESCs) may produce that enhance regeneration and if those factors have any effects by themselves on regeneration. (ca.gov)
Our current running hypothesis is that the positive growth factors produced by hESCs trigger injured muscle to initiate and maintain regeneration, the TGF-beta inhibitors produced by hESCs reduce the TGF-beta signaling, and the combination assures the robust regeneration of muscle. (ca.gov)
Preliminary data indicates the effects on regeneration of old muscle look very promising. (ca.gov)
Specifically, our data suggest that several other ligands of MAPK pathway secreted by hESCs are likely to enhance and rejuvenate the regeneration of old muscle tissue. (ca.gov)
We have submitted the several manuscripts on topics of enhanced tissue regeneration and we are preparing the manuscript that identifies hESC-based novel strategies for restoring high regenerative capacity to old muscle. (ca.gov)
Finally, our data suggest that muscle stem cells either do not accumulate DNA damage with age or can efficiently repair such damage, when activated for tissue regeneration. (ca.gov)
In particular, her work has been focusing on the molecular mechanisms involved in the modulation of the peripheral synaptic transmission, skeletal muscle adaptation and regeneration in physiological and pathological conditions. (units.it)
The research activity of the group is focused on the identification of new strategies to improve skeletal muscle regeneration. (units.it)
Among Ca 2+ -sensitive pathways, calcineurin is essential for myoblast differentiation and muscle regeneration. (biologists.com)
Defective muscle regeneration can severely affect re-innervation by motor axons, and neuromuscular junctions (NMJs) development, ultimately leading to skeletal muscle atrophy. (biomed.news)
In particular, in skeletal muscle, specific semaphorin molecules are involved in the restoration and remodeling of the nerve-muscle connections, thus emphasizing their plausible role to ensure the success of muscle regeneration. (biomed.news)
This review article aims to discuss the impact of aging on skeletal muscle regeneration and NMJs remodeling and will highlight the most recent insights about the role of semaphorins in this context. (biomed.news)
Epimorphic regeneration can be thought of as occurring in two phases: wound healing and cell proliferation. (biomedcentral.com)
Therefore, promotion of myoblast proliferation and differentiation and induction of myotube hypertrophy should be beneficial for muscle regeneration and control of muscle mass. (kosfaj.org)

More specifically, upon activation, satellite cells can re-enter the cell cycle to proliferate and differentiate into myoblasts. (wikipedia.org)
Activated satellite cells initially proliferate as skeletal myoblasts before undergoing myogenic differentiation. (wikipedia.org)
Time-lapse live imaging reveals that during early craniofacial development, myoblasts condense into round clusters corresponding to future muscle groups. (bvsalud.org)
Skeletal muscle progenitors (satellite cells), murine cell line and immortalized human myoblasts are studied in vitro to identify, in a controlled environment, the pathways regulating the regenerative potential of the skeletal muscle. (units.it)
Ensure high yields when passaging and culturing your primary human skeletal muscle progenitor cells (myoblasts) by using MyoCult™-SF Attachment Substrate, which is optimized to support myoblast attachment. (stemcell.com)
human pluripotent stem cell (hPSC)-derived myoblasts may be cultured on cultureware coated with Corning® Matrigel® hESC-Qualified Matrix. (stemcell.com)
Myoblasts cultured using MyoCult™-SF Expansion Medium can be differentiated into multinucleated myotubes using MyoCult™ Differentiation Kit (Human) . (stemcell.com)
Using synchronized cultures of C2C12 myoblasts to model these divergent programs, we show that p8 (also known as Nupr1 ), a G1-induced gene, negatively regulates the cell cycle and promotes myogenic differentiation. (biologists.com)
These monocyte myoblasts proliferate and differentiate, following which, they fuse with existing muscle fibers to form multinucleated myotubes and myofibers. (kosfaj.org)

There is a longstanding belief that a given nucleus controls a defined volume of cytoplasm, so when a muscle grows (hypertrophy) or shrinks (atrophy), the number of myonuclei change accordingly. (frontiersin.org)
Recently, two independent models, one from rodents and the other from insects, have demonstrated that nuclei are not lost from skeletal muscle fibers when they undergo either atrophy or programmed cell death. (frontiersin.org)
Obestatin signalling counteracts glucocorticoid-induced skeletal muscle atrophy via NEDD4/KLF15 axis. (institut-myologie.org)
This assumption long seemed valid, with many researchers reporting the presence of disintegrating nuclei in muscle tissue during atrophy induced by inactivity, injury or paralysis. (robert-gorter.info)
Muscles get damaged during extreme exercise, and often have to weather changes in food availability and other environmental factors that lead to atrophy. (robert-gorter.info)
The effect of deer antler extract on muscle differentiation and muscle atrophy were evaluated to minimize muscle loss following aging. (kosfaj.org)
In addition, the expression levels of genes related to muscle differentiation and atrophy were confirmed through qRT-PCR. (kosfaj.org)
Gene expression of AMP-activated protein kinase (AMPK), MyoD1, and myogenin, along with the muscle atrophy factors muscle RING finger-1 (MuRF-1) and forkhead box O3a (FoxO3a) upon addition of deer antler extracts to muscle-atrophied C2C12 cells was determined by qRT-PCR after treatment with AICAR. (kosfaj.org)
In addition, gene expression of MyoD1 and myogenin in the muscle atrophy cell model was significantly increased compared that into the CON. (kosfaj.org)
Therefore, our findings indicate that antler extract can increase the expression of MyoD1, Myf5 and myogenin, inhibit muscle atrophy, and promote muscle differentiation. (kosfaj.org)
Muscle atrophy with aging is accompanied by muscle damage caused by oxidative damage and malnutrition caused by lack of muscle metabolism. (kosfaj.org)

The purpose of this brief review is to discuss whether or not ambulatory exercise elicits leg muscle hypertrophy in older adults. (researchgate.net)
In addition, slow-walk training with a combination of leg muscle blood flow restriction elicits muscle hypertrophy only in the blood flow restricted leg muscles. (researchgate.net)
Competitive marathon running and regular high intensity distance running in young and middle-aged adults may not produce leg muscle hypertrophy due to insufficient recovery from the damaging running bout, although there have been no studies that have investigated the effects of running on leg muscle morphology in older subjects. (researchgate.net)
It is clear that skeletal muscle hypertrophy can occur independently of exercise mode and load. (researchgate.net)
There is a general agreement that hypertrophy is accompanied by the addition of new nuclei from stem cells to help the muscles meet the enhanced synthetic demands of a larger cell. (frontiersin.org)
High levels are associated with muscle hypertrophy, whereas low levels are epidemiologically associated with metabolic syndrome and diabetes, which negatively impact muscle functions. (frontiersin.org)

The use of a muscle creatine kinase germline knockout of the gene encoding Ire1/Ern1 shifts the focus from traditional studies of tissue repair toward the regenerating myofibers. (elifesciences.org)
There is also evidence suggesting that these cells are capable of fusing with existing myofibers to facilitate growth and repair. (wikipedia.org)
Satellite cells proliferate following muscle trauma and form new myofibers through a process similar to fetal muscle development. (wikipedia.org)
Intro The regenerative response of skeletal muscle mass to ICAM4 injury is dependent within the quiescent populace of skeletal muscle mass stem cells termed the satellite cells which reside beneath the basal lamina of each myofiber (Brack and Rando 2012 Wang and Rudnicki 2012 Upon injury these quiescent satellite cells become triggered and undergo proliferation providing rise to myogenic progenitors (MPs) that ultimately differentiate into mature myofibers. (scienceexhibitions.org)

Activation of IL-4/IL-13 signaling promotes proliferation of FAPs to support myogenesis while inhibiting their differentiation into adipocytes. (scienceexhibitions.org)
MicroRNA-1/206(miRNA-1/206) is specifically expressed in skeletal muscle and play a critical role in myogenesis. (biomedcentral.com)
Cell culture experiments indicated that Gln could promote cell proliferation in a dose dependent manner, but expression of myogenesis regulatory genes was not altered. (nature.com)
In more detail, the team is studying the role of electrical activity and local trophic factors in the microenvironment of satellite cell niche and in the post-mitotic myogenesis. (units.it)
Adult regenerative myogenesis is dependent on progenitor cells. (biologists.com)

Myosatellite cells are located between the basement membrane and the sarcolemma of muscle fibers, and can lie in grooves either parallel or transversely to the longitudinal axis of the fibre. (wikipedia.org)
Satellite cells are able to differentiate and fuse to augment existing muscle fibers and to form new fibers. (wikipedia.org)
The close association of FAPs with regenerating muscle mass fibers along with their manifestation of factors that influence myogenic differentiation such as IL-6 and IGF-1 suggests that these stromal cells may perform a supportive part in myogenic differentiation (Joe et BV-6 al. (scienceexhibitions.org)
Until recently, scientists thought this meant that nuclei - the cell control centers that build and maintain muscle fibers - are also lost to sloth. (robert-gorter.info)
Two independent studies - one in rodents and the other in insects - have demonstrated that nuclei are not lost from atrophying muscle fibers, and even remain after muscle death has been initiated. (robert-gorter.info)
RACK1 was expressed transiently in the skeletal muscle of post-natal mice, being abundant in the early phase of muscle growth and almost disappearing in adult mature fibers. (sdbonline.org)
After acute injury in muscle of both mouse and the fruit fly Drosophila melanogaster (used as alternative in vivo model) this study found that RACK1 accumulated in regenerating fibers while it declined with the progression of repair process. (sdbonline.org)
Diprotin A stimulation of CXCR4, however, significantly increased the number of canine dystrophin-positive muscle fibers and canine-derived satellite cells by enhancing donor cell proliferation. (biomedcentral.com)

The expression level of the SFRP1 was significantly higher in the embryonic skeletal compared with postnatal skeletal muscle, whereas miR-206 showed the inverse pattern of expression. (biomedcentral.com)
Our work is at the stage of understanding the molecular mechanisms by which the aging of the regenerative potential of organ stem cells can be reversed by particular human embryonic factors that are capable of neutralizing the affects of aged niches on tissue regenerative capacity. (ca.gov)
In this project, the group intends to investigate the nature and the role of adenosine and AR signalling pathways on the two isoforms of muscle AChRs: the embryonic (g-AChR) regulating synaptogenesis and muscle development and the adult (e-AChR) with a role in nerve-muscle communication. (units.it)
Embryonic myogenic progenitor cells express CXCR4, G-protein coupled cell surface receptor, and migrate towards regions of SDF-1 expression during limb muscle development, suggesting that CXCR4/SDF-1 plays a role in muscle cell homing. (biomedcentral.com)
Electrical stimulation of human embryonic stem cells: Cardiac differentiation and the generation of reactive oxygen species. (unipd.it)

Here, we demonstrate that the developing skeleton instructs the directional outgrowth of skeletal muscle and other soft tissues during limb and facial morphogenesis in zebrafish and mouse. (bvsalud.org)
The expression level of the SFRP1 was highly varied across numerous pig tissues and it was down-regulated during porcine skeletal muscle development. (biomedcentral.com)
Feeding with amino acids can stimulate protein synthesis in all tissues, especially in skeletal muscle as reviewed by Davis et al. (nature.com)
What was surprising is that administering these inhibitors to the whole animal appears to reduce TGF-beta levels in the whole animal, suggesting some kind of feed-back and perhaps effects on other tissues as well as muscle. (ca.gov)
The poor performance of stem cells in an aged organ, such as skeletal muscle, is caused by the changes in regulatory pathways such as Notch, MAPK and TGF‐β, where old differentiated tissues and blood circulation inhibit the regenerative performance of organ stem cells. (ca.gov)
Jyotsna Dhawan ABSTRACT Adult stem cells persist in mammalian tissues by entering a state of reversible quiescence, referred to as G 0 , which is associated with low levels of transcription. (biologists.com)
The lab is also pursuing the role of physical activity/exercise as a time cue for skeletal muscle and other tissues. (ufl.edu)
Originally discovered as axon guidance factors, they have been implicated in cancer progression, embryonal organogenesis, skeletal muscle innervation, and other physiological and developmental functions in different tissues. (biomed.news)
Once the wound is healed, the cells of the stump must mobilise under the wound epidermis and begin the process of replacing lost tissues, by forming a proliferating blastema. (biomedcentral.com)
A central aspect is then the ability to optimize the functional properties with high spatial resolution, creating materials that are able to control the interaction with the biological surrounding at the nanoscale thus guiding the responses of biomolecules, cells and tissues. (european-mrs.com)
Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers. (u-paris.fr)

Immortalized Canine Dystrophic Myoblast Cell Lines for Development of Peptide-Conjugated Splice-Switching Oligonucleotides. (institut-myologie.org)
Stephane Koenig ABSTRACT Ca 2+ signaling plays a key role during human myoblast differentiation. (biologists.com)
Our results showed that miR-195 and miR-497 were upregulated during muscle development and myoblast differentiation. (biologists.com)

Myofiber-specific ablation of IRE1α dampens Notch signaling and canonical NF-κB pathway in skeletal muscle of adult mice. (elifesciences.org)
The dynamics of RACK1 levels in isolated adult SC of mice, i.e., progressively high during differentiation and low compared to proliferating conditions, and RACK1 silencing indicated that RACK1 promotes both the formation of myotubes and the accretion of nascent myotubes. (sdbonline.org)
In 2010 beginning of 2011, we have approached the identification and characterization of the proteins that are produced by hESCs and have the rejuvenating and pro-regenerative activity on adult muscle. (ca.gov)
In murine-to-murine transplantation experiments, CXCR4 expression marks a population of adult murine satellite cells with robust engraftment potential in mdx mice, and CXCR4-positive murine muscle-derived SP cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice. (biomedcentral.com)
Adult stem cells play key roles in homeostasis and tissue repair. (biomed.news)
We observed that, as skeletal muscle progressed from perinatal to postnatal and adult developmental stages, MSY3 protein became gradually dephosphorylated and accumulated in the nucleus. (biomedcentral.com)
Furthermore, forced expression of Akt in adult skeletal muscle induced MSY3 phosphorylation and myogenin derepression. (biomedcentral.com)
Given the significance of myogenin regulation in adult muscle, the Akt/MSY3/myogenin regulatory circuit is a potential therapeutic target to counteract muscle degenerative disease. (biomedcentral.com)

Cancer-associated cachexia (CAC) is a multifactorial wasting syndrome characterized by loss of skeletal muscle. (bvsalud.org)
Sarcopenia, the age-related loss of skeletal muscle mass and function, is becoming more prevalent as the lifespan continues to increase in most populations. (frontiersin.org)
The term sarcopenia was first proposed in 1989 by Irwin Rosenberg to describe a multifactorial syndrome that occurs with age and results in loss of skeletal muscle mass and function ( 3 , 4 ). (frontiersin.org)

Skeletal muscle, the most abundant tissue of the body, has remarkable regenerative capacity mainly due to its resident muscle stem cells, also known as satellite cells. (elifesciences.org)
Fibroblasts within the muscle deposit scar tissue, which can impair muscle function, and is a significant part of the pathology of muscular dystrophies. (wikipedia.org)
2010 However reflecting their adipogenic potential FAPs can also give rise to ectopic adipocytes that accumulate in degenerating muscle tissue (Uezumi et al. (scienceexhibitions.org)
Muscle injury results in quick activation of the innate immune system which exerts pleiotropic effects on regenerating muscle mass (Brunelli and Rovere-Querini 2008 Tidball and Villalta 2010 Within minutes of injury neutrophils infiltrate hurt skeletal muscle mass and launch tissue-damaging reactive molecules which exacerbate muscle mass damage (Tidball 1995 This initial burst of security damage caused by the innate immune system is followed by a wave of reparative macrophages. (scienceexhibitions.org)
However, since there are many different non-muscle cell populations that reside within the tissue, these experiments cannot easily distinguish true myonuclei from those of neighboring mononuclear cells. (frontiersin.org)
Skeletal muscle is the largest tissue in the body. (frontiersin.org)
More proliferation was observed in muscle tissue of LBW-GLN than LBW-ALA piglets at 5 dpn, but there was no clear effect of supplementation on related gene expression. (nature.com)
Overall, Gln supplementation stimulated cell proliferation in muscle tissue and in vitro in myogenic cell culture, whereas muscle growth regulatory genes were barely altered. (nature.com)
Glutamine is regarded as the most abundant amino acid in the body, is mainly synthesized in skeletal muscle 10 , and muscle tissue is the most important site for Gln storage 11 . (nature.com)
Skeletal muscle is a target tissue for anabolic steroids. (frontiersin.org)
It's actually a special type of tissue in your body, where cells are fused together extra close - so close, that they behave a like a giant single cell. (robert-gorter.info)
In this paper, we discuss the current available information about the role that different phenotypes of macrophages plays after injury and during the remodelling phase in different tissue types, with particular attention to the skeletal muscle. (hindawi.com)
The levels of catalase (CAT) and glutathione (GSH) were measured in PC12 cells and Drosophila brain tissue. (sdbonline.org)
GSOs also mitigated the deleterious effects of GLU on the mitochondrial membrane potential and Cyt C release, thus alleviating mitochondrial dysfunction, and increased GSH levels and CAT activity in both cells and Drosophila brain tissue. (sdbonline.org)
Thus, the use of hESC-produced pro-regenerative factors for boosting the regenerative capacity of organ stem cells is likely to yield healthy, young tissue. (ca.gov)
Although functional organ stem cells persist in the old, tissue damage invariably overwhelms tissue repair, ultimately causing the demise of an organism. (ca.gov)
She has been reviewer for international Journals ( FASEB J , Cell & Tissue Res , Toxicon, Exp Cell Res ), for national grant agencies (MIUR) and international Foundations (AFM, Association Francaise contre les Myopathies). (units.it)
With ageing, skeletal muscle undergoes a severe reduction in tissue mass, leading to a decrease in strength (sarcopenia). (units.it)
In recent years, he has been studying cell-cell interaction by single-cell analysis to clarify the factors that disrupt tissue homeostasis. (tmd.ac.jp)
The compromised tissue repair program is attributable to the gradual depletion of stem cells and to altered regulatory signals. (biomed.news)

Our research goals are to define the transcriptional networks and downstream mechanisms that link the molecular clock with proper skeletal muscle function and phenotype. (ufl.edu)

In vivo , the identification of macrophage phenotype is complicated since macrophages are obviously exposed to a microenvironment that is more complex respect to cell culture conditions, and they display characteristics that do not conform to the in vitro defined phenotypic categories. (hindawi.com)

However, the impact of IL11 on muscle metabolism remains to be determined. (bvsalud.org)
High-fat, low-carbohydrate (CHO) diets increase whole-body rates of fat oxidation and down-regulate CHO metabolism. (edu.au)

The main interest of her research activity is the nerve-muscle communication and the skeletal muscle physiology, including muscle plasticity and impact of ageing on the neuromuscular system. (units.it)
The results of the proposed research will advance the understanding of important aspects of the neuromuscular physiology such as the modulation of the neuromuscular transmission and the skeletal muscle plasticity. (units.it)

2009 potentially reflecting the reduced secretion of myogenic growth element IGF-1 by these cells (Wynes and Riches 2003 While these studies demonstrate a facilitative part of innate immune cells in muscle mass regrowth after injury a direct molecular link between the innate immune system and muscle mass progenitor biology remains to be founded. (scienceexhibitions.org)
For more information on protocols for the derivation and expansion of human skeletal muscle progenitor cells with MyoCult™, please explore the Product Information Sheet . (stemcell.com)

Transplantation of myogenic stem cells possesses great potential for long-term repair of dystrophic muscle. (biomedcentral.com)

Quantifying the exact expression levels of the mRNA isoforms encoding these myogenic transcription factors is therefore key to understanding how MuSCs switch between cell states. (biomed.news)
We demonstrate that mdPCR can give exact molecule counts per cell for mRNA isoforms encoding the myogenic transcription factor Pax3. (biomed.news)

they neither differentiate nor undergo cell division. (wikipedia.org)
When muscle cells undergo injury, quiescent satellite cells are released from beneath the basement membrane. (wikipedia.org)
After several cell divisions, the satellite cells begin to fuse with the damaged myotubes and undergo further differentiations and maturation, with peripheral nuclei as in hallmark. (wikipedia.org)

Therefore, we complemented our investigations into the cellular development of the skeletal muscle of neonatal piglets with in vitro studies using a primary porcine myogenic cell culture model with Gln supplementation. (nature.com)
Then, we comprehensively assessed the functional and histological parameters of skeletal muscle injury in mice and the phenotype shifting in macrophages through molecular biological methods and immunofluorescence analysis both in vivo and in vitro . (ijbs.com)
Skeletal muscle and myogenic C2C12 cells were used to study the effects of MSY3 phosphorylation in vivo and in vitro on its sub-cellular localization and activity, by blocking the IGF1/PI3K/Akt pathway, by Akt depletion and over-expression, and by mutating potential MSY3 phosphorylation sites. (biomedcentral.com)

Thus, we provide the first concrete evidence that PIEZO1, a bona fide mechanosensitive ion channel, promotes proliferation and regenerative functions of MuSCs through precise control of cell division. (bvsalud.org)

As well, many older individuals suffer from sarcopenia, a protracted muscle wasting disorder that typically begins after the age of 50 and involves a loss of about 1% of muscle mass per year ( Woo, 2017 ). (frontiersin.org)
Significant loss of muscle mass may occur in cachexia and sarcopenia, which are major causes of mortality and disability. (mdpi.com)
Testosterone concentrations decline as age increase, suggesting that low plasma testosterone levels can cause or accelerate muscle- and age-related diseases, as sarcopenia. (frontiersin.org)
Currently, there is increasing interest on the anabolic properties of testosterone for therapeutic use in muscle diseases including sarcopenia. (frontiersin.org)
This age-related decline in skeletal muscle mass and strength generation, the primary function of skeletal muscle mass, is known as sarcopenia ( 2 , 3 ). (frontiersin.org)
Indeed, most of the intrinsic as well as extrinsic (systemic) muscle changes that occur with age are believed to be involved in the development of sarcopenia ( 5 , 6 ). (frontiersin.org)
However, during aging, extensive injury, or pathological conditions, the complete regenerative program is severely affected, with a progressive loss of muscle mass and function, a condition known as sarcopenia. (biomed.news)

However, the mechanisms that drive myocyte orientation and fusion to control muscle directionality in adults remain enigmatic. (bvsalud.org)
These cell lines are shared in collaboration with many international teams, either to study physiological or pathophysiological mechanisms, or to test innovative therapeutic approaches. (institut-myologie.org)
However, the pathophysiological mechanisms underlying this muscle syndrome and its relationship with plasma level of androgens are not completely understood. (frontiersin.org)
Additionally, our data in progress suggest that muscle and brain age by similar molecular mechanisms and thus, therapeutic strategies for rejuvenating muscle repair might be applicable to the restoration of neurogenesis in aged brain. (ca.gov)
Laboratory work has centered on the mechanisms of how cells acquire specific fates during growth and development, providing insights into normal and abnormal mechanisms of growth control. (stanford.edu)
Possible MSY3 mechanisms of action have been studied in cell culture studies. (biomedcentral.com)

In addition, IL11 treatment reduced the protein and mRNA levels of myosin heavy chain (MHC), a marker of myogenic differentiation. (bvsalud.org)
The miRNA-mRNA profiles and bioinformatics study suggested that the SFRP1 gene was potentially regulated by miRNA-1/206 during porcine skeletal muscle development. (biomedcentral.com)
In this study, we examined whether AdipoR mRNA expression in human myotubes correlates with in vivo measures of insulin sensitivity. (diabetesjournals.org)
Myotubes from 40 metabolically characterized donors expressed 1.8-fold more AdipoR1 than AdipoR2 mRNA (588 ± 35 vs. 321 ± 39 fg/μg total RNA). (diabetesjournals.org)
Panobinostat treatment drastically repressed mRNA for DNMT1 and DNMT3a in the two cell lines whilst no adjustments have been observed in DNMT3b levels. (ilreceptor.com)

The field of satellite cell biology suffers from the same technical difficulties as other stem cell fields. (wikipedia.org)
KIT is a receptor tyrosine kinase type III, which binds to stem cell factor. (inhibitorkit.com)
Therefore, these results establish the efficacy of diprotin A in stimulating muscle cell engraftment, and highlight the pre-clinical utility of a xenotransplantation model in assessing the relative efficacy of muscle stem cell populations. (biomedcentral.com)
We used a clinically acceptable regimen of hematopoietic stem cell transplantation to establish mixed donor/host blood cell chimerism and immune tolerance in a canine model of Duchenne muscular dystrophy ( cxmd ) [ 9 ]. (biomedcentral.com)
Hiroyuki Koike is an Associate Professor in the Department of Medical Biochemistry, Tokyo Medical and Dental University, specializing in stem cell biology and developmental biology. (tmd.ac.jp)

CD34 and Myf5 markers specifically define the majority of quiescent satellite cells. (wikipedia.org)

The SFRPs inhibit Wnt receptor binding to down-regulate pathway signaling during development [ 8 ]. (biomedcentral.com)

The intent of vaccination is to induce a combined antibody and T-cell anti-HER-2 immune response, which is intended to target HER-2-expressing tumor cells, and may induce tumor regression or slow progression of disease. (stanford.edu)

Such lack of clarity can be attributed to difficulty in isolating the individual events responsible for alterations in skeletal muscle, most of which occur simultaneously, among the multiple age-associated changes and co-morbidities associated with advanced age. (frontiersin.org)

Our results indicate that the SFRP1 gene is regulated by miR-1/206 and potentially affects skeletal muscle development. (biomedcentral.com)
Animals with a polymorphism in the myostatin gene that renders it less active have hypertrophied muscles. (silverchair.com)
In the presence of antler extracts, the length and thickness of myotubes and myogenin differentiation 1 (MyoD1) and myogenic factor 5 (Myf5) gene expression were increased compared to those in the control group (CON). (kosfaj.org)

Myosatellite cells, also known as satellite cells, muscle stem cells or MuSCs, are small multipotent cells with very little cytoplasm found in mature muscle. (wikipedia.org)
For example, muscle stem cells (MuSCs), located beneath the basal lamina, exist in the quiescent state but can transition to an activated, proliferative state upon injury. (biomed.news)
Primary MuSCs isolated from individual skeletal muscles (gastrocnemius and masseter) are lysed, and their RNA is reverse-transcribed into cDNA and copied into double-stranded DNA. (biomed.news)

Additionally, pretreatment with PD98059 and U0126 resulted in improved myotube formation and reduced BrdU staining in IL11-treated cells. (bvsalud.org)
Additionally we are working in mouse and human models to determine the potential for time of day lifestyle interventions as a therapeutic approach to enhance molecular clock function and attenuate conditions of muscle weakness and wasting seen with aging and chronic diseases. (ufl.edu)

Treatment of C2C12 cells with recombinant mouse IL11 resulted in decreased myotube formation. (bvsalud.org)
IL11 treatment increased the number of BrdU-positive cells and the level of phosphorylated retinoblastoma (Rb) protein, while the levels of p21Waf1 and p27Kip1 were reduced by IL11 treatment in differentiating C2C12 cells, suggesting that IL11 interferes with cell cycle exit during the early stages of myogenic differentiation. (bvsalud.org)
IL11 treatment resulted in an activation of ERK, STAT3, and AKT in differentiating C2C12 cells. (bvsalud.org)
In C2C12 myogenic cells, blocking the IGF1/PI3K/Akt pathway using LY294002 inhibitor reduced MSY3 phosphorylation levels resulting in its accumulation in the nuclei. (biomedcentral.com)

Ex vivo cultures of myofiber explants demonstrate that ablation of IRE1α reduces the proliferative capacity of myofiber-associated satellite cells. (elifesciences.org)
Studies targeted at establishing such effects at cellular level and their correlations with in vivo models, will broaden our understanding of the roles played by androgens on skeletal muscle function in elderly. (frontiersin.org)
The comprehensive functional tests and histological analysis in vivo indirectly and directly proved the effectiveness of LIPUS for muscle recovery. (ijbs.com)
Depletion of macrophages in vivo eliminated the therapeutic effects of LIPUS, indicating that improvement in muscle function was the result of M2-shifted macrophage polarization. (ijbs.com)
However, diprotin A, a positive modulator of CXCR4-SDF-1 binding, significantly enhanced engraftment and stimulated sustained proliferation of donor cells in vivo . (biomedcentral.com)

Indeed, CXCR4-positive muscle-derived side population (SP) cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice [ 10 ]. (biomedcentral.com)

Overall, this work outlines a biomechanical guidance mechanism that is potentially useful for engineering functional skeletal muscle. (bvsalud.org)
We reported that LIPUS therapy at intensity of 60mW/cm 2 exhibited the most significant differences in functional recovery of contusion-injured muscle in mice. (ijbs.com)
Several types of adenosine receptors have been detected in developing and differentiated skeletal muscle cells, but little is known about the functional outcome of AR modulation on muscle AChRs. (units.it)

J Cell Sci (2022) 135 (15): jcs259789. (biologists.com)

We have also shown that time of exercise can significantly alter the clock mechanism in skeletal muscle independent of lighting and the central clock in the brain. (ufl.edu)

For instance it has been proposed that classically triggered (M1) macrophages infiltrate early to facilitate the clearance of necrotic debris whereas alternatively triggered (M2) macrophages infiltrate later on to assist with muscle growth (Arnold et al. (scienceexhibitions.org)
Alternatively activated M2 macrophages comprise cells exposed to low concentrations of M-CSF in the presence of IL-4, IL-13, or IL-10. (hindawi.com)

The Wnt signaling pathway is involved in the control of cell proliferation and differentiation during skeletal muscle development. (biomedcentral.com)
dGLYAT was found to regulate Gadd45 -mediated JNK pathway activation and cell invasion. (sdbonline.org)
Firstly, loss of dGLYAT suppressed scrib depletion- or Egr overexpression-induced JNK pathway activation and invasive cell migration. (sdbonline.org)
This study highlights a previously undescribed Akt-mediated signaling pathway involved in the repression of myogenin expression in myogenic cells and in mature muscle. (biomedcentral.com)

Our previous studies showed that Gln had the potential to increase muscle fiber size in piglets 16 , but the mechanism was not clear. (nature.com)
This study aims to understand the function and mechanism of Drosophila Glycine N-acyltransferase (GLYAT) in cell invasion. (sdbonline.org)
My lab has pioneered research on the role of circadian rhythms and the molecular clock mechanism in skeletal muscle homeostasis and health. (ufl.edu)

In this study, we used a canine-to-murine xenotransplantation model to quantitatively compare canine muscle cell engraftment, and test the most effective cell population and modulating factor in a canine model of DMD using allogeneic transplantation experiments. (biomedcentral.com)
Furthermore, the canine-to-murine xenotransplantation model accurately predicted results in canine-to-canine muscle cell transplantation. (biomedcentral.com)
Yet, it remains unclear from murine transplantation experiments which cell population most effectively engrafts into diseased skeletal muscle. (biomedcentral.com)

With this context of injury and repair a number of factors have been recognized that promote proliferation and differentiation of MPs (Kuang et al. (scienceexhibitions.org)

Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)

Skeletal muscle is fairly unique in that the mature cells are syncytial and can contain hundreds of nuclei. (frontiersin.org)
But according to a review published in Frontiers in Physiology, modern lab techniques now allow us to see that nuclei gained during training persist even when muscle cells shrink due to disuse or start to break down. (robert-gorter.info)
Muscle growth is accompanied by the addition of new nuclei from stem cells to help meet the enhanced synthetic demands of larger muscle cells," explains Schwartz. (robert-gorter.info)
But modern cell-type-specific dyes and genetic markers have shown that the dying nuclei other researchers had detected were in fact inflammatory and other cells recruited to atrophic muscle. (robert-gorter.info)

Current thinking is that most satellite cells express PAX7 and PAX3. (wikipedia.org)
Satellite cells in the head musculature have a unique developmental program, and are Pax3-negative. (wikipedia.org)

Together, our results suggest that IL11 inhibits myogenic differentiation through delayed cell cycle exit in an ERK-dependent manner. (bvsalud.org)

As such, the findings have important implications beyond understanding muscle biology. (robert-gorter.info)
These findings had been corroborated by westernblot analysis displaying a powerful reduction of DNMT1 and DNMT3a protein in each cell lines but not of DNMT3b. (ilreceptor.com)

Satellite cells are precursors to skeletal muscle cells, able to give rise to satellite cells or differentiated skeletal muscle cells. (wikipedia.org)
Our team has demonstrated some important differences between man and mouse, in the regulation of the proliferation of myogenic precursors. (institut-myologie.org)
Therefore, in collaboration with W. Wright, we have developed an approach for the immortalisation of human muscle precursors that allows them to retain their ability to differentiate: this has enabled the generation of immortal cell lines derived from patient cells. (institut-myologie.org)

They have proinflammatory functions: they produce effector molecules (reactive oxygen and nitrogen intermediates) and inflammatory cytokines (IL-1 β , TNF α , and IL-6) and participate as inducer and effector cells in polarized Th1 responses. (hindawi.com)
IL-2/IL2R also promotes the differentiation of T cells into effector T cells. (ilreceptor.com)

It is well documented in the field of exercise physiology that it is far easier to reacquire a certain level of muscle fitness through exercise than it was to achieve it the first place, even if there has been a long intervening period of detraining. (robert-gorter.info)

These results also suggest the coupling of RACK1 to muscle unfolded protein response during SC activation. (sdbonline.org)
We studied the involvement of protein kinases A and C, phospho inositol 3 kinase, extracellu lar regulated kinases , vesicular release of dopamine, and changes in intracellular Ca2 concentra tions in the actions of estrogens. (inhibitorkit.com)
Right here, only a transient decrease in protein levels was observed right after 24 to 48 h in both cell lines. (ilreceptor.com)
Mammalian hibernation presents a unique scenario, where animals are hypocaloric and in torpor, but the extent of muscle protein loss is minimized. (silverchair.com)
however, priority needs to be given to resistance exercise training, and secondarily to adequate protein intake should be part of this dietary regime to mitigate losing muscle mass. (biomed.news)
The Y-box protein MSY3/Csda represses myogenin transcription in skeletal muscle by binding a highly conserved cis-acting DNA element located just upstream of the myogenin minimal promoter (myogHCE). (biomedcentral.com)

in these cells physiological lev els of E2 and low levels of xenoestrogens can rapidly reverse actions of the DAT. (inhibitorkit.com)
Skeletal muscle is characterized by a remarkable capacity to rearrange after physiological changes and efficiently regenerate. (biomed.news)

Knocking down Akt expression increased the amount of dephosphorylated MSY3 and reduced myogenin expression and muscle differentiation. (biomedcentral.com)
While Akt over-expression rescued myogenin expression in MSY3 overexpressing myogenic cells, ablation of the Akt substrate, (Ser126 located in the MSY3 cold shock domain) promoted MSY3 accumulation in the nucleus and abolished this rescue. (biomedcentral.com)
These results support the hypothesis that MSY3 phosphorylation by Akt interferes with MSY3 repression of myogenin circuit activity during muscle development. (biomedcentral.com)
We have previously shown that MSY3/Csda binds a highly conserved cis-acting element located upstream of the myogenin promoter (myogHCE) and regulates the postnatal down-regulation of myogenin [ 16 ]. (biomedcentral.com)

These cells are located beneath the basal lamina of myofiber in a dormant state. (elifesciences.org)

Anatomy61

Organisms16

Diseases12

Chemicals and Drugs84

Analytical, Diagnostic and Therapeutic Techniques and Equipment33

Phenomena and Processes52

Disciplines and Occupations2

Information Science4

Health Care1

Regeneration36

Myoblasts9

Atrophy11

Hypertrophy6

Myofibers4

Myogenesis5

Fibers8

Embryonic5

Tissues11

Myoblast3

Adult8

Loss of skeletal muscle3

Tissue20

Proper skeletal muscle1

Microenvironment1

Metabolism2

Plasticity2

Progenitor2

Myogenic stem cells1

Isoforms2

Undergo3

Vitro3

Promotes proliferation1

Sarcopenia7

Mechanisms6

MRNA5

Stem cell5

Quiescent satellite cells1

Receptor1

Progression1

Occur1

Gene3

MuSCs3

Additionally2

C2C124

Vivo5

Dystrophic1

Functional3

20221

Significantly1

Macrophages2

Pathway4

Mechanism3

Murine3

Kuang1

Apoptosis1

Nuclei4

PAX32

Inhibits1

Findings2

Precursors3

Effector2

Physiology1

Protein6

Physiological2

Myogenin4

Located beneath the basal lamina1