• This study was therefore aimed at establishing the dose-response effect of EPO stimulation on rat BMSCs and investigating the effects of mechanical stretch combined with EPO on the proliferation and osteogenic differentiation of BMSCs. (hindawi.com)
  • The proliferation and osteogenic differentiation of rat BMSCs were examined and compared using EPO with different concentrations. (hindawi.com)
  • The osteogenic differentiation of BMSCs was detected by alkaline phosphatase (ALP) staining, and the mRNA expression levels of ALP, OCN, COL, and Runx2 were detected by qPCR. (hindawi.com)
  • Mechanical stretch combined with 20 IU/ml EPO promoted proliferation and osteogenic differentiation of BMSCs. (hindawi.com)
  • EPO was able to promote the proliferation and osteogenic differentiation of BMSCs, and these effects were enhanced when combined with mechanical stretch. (hindawi.com)
  • Notably, it promotes the osteogenic differentiation of bone mesenchymal stem cells (BMSCs) [ 15 - 17 ]. (hindawi.com)
  • This study was therefore aimed at addressing three key issues: First, is there a dose-dependent effect of EPO on the proliferation and osteogenic differentiation of BMSCs? (hindawi.com)
  • In this study, we verified that exosomes derived from osteogenically differentiated ADSCs can promote osteogenic differentiation of ADSCs. (hindawi.com)
  • Moreover, we found that these differentially expressed exosomal miRNAs connect osteogenic differentiation to processes such as axon guidance, MAPK signaling, and Wnt signaling. (hindawi.com)
  • This study confirms that alterations in the expression of exosomal miRNAs can promote osteogenic differentiation of ADSCs, which also provides the foundation for further research on the regulatory functions of exosomal miRNAs in the context of ADSC osteogenesis. (hindawi.com)
  • Zhou JQ, Wan HY, Wang ZX, Jiang N. Stimulating factors for regulation of osteogenic and chondrogenic differentiation of mesenchymal stem cells. (wjgnet.com)
  • Recently, miRNA-messenger RNA (mRNA) regulatory relationships have been confirmed during biological processes, including osteogenic differentiation. (frontiersin.org)
  • This study aimed to find out more candidate miRNA-mRNA pairs involved in the osteogenic differentiation of MC3T3-E1 cells. (frontiersin.org)
  • The MC3T3-E1 cells were cultured and incubated in the osteogenic differentiation medium for 14 days. (frontiersin.org)
  • There were 7 overlapped miRNA-mRNA pairs identified during osteogenic differentiation of MC3T3-E1 cells, including mmu-miR-204-5p-Arhgap11a, mmu-miR-211-5p-Arhgap11a, mmu-miR-24-3p-H2afx, mmu-miR-3470b-Chek2, mmu-miR-3470b-Dlgap5, mmu-miR-466b-3p-Chek1, and mmu-miR-466c-3p-Chek1. (frontiersin.org)
  • The Arhgap11a, H2afx, Chek2, Dlgap5, and Chek1 were hub genes downregulated in MC3T3-E1 cells after osteogenic differentiation, verified by RT-qPCR results. (frontiersin.org)
  • Our study characterizes miR-211-5p targeting Arhgap11a promotes the osteogenic differentiation of MC3T3-E1 cells, which provides novel targets to promote the osteogenesis process during bone repair. (frontiersin.org)
  • Such cells from different sources can proliferate and differentiate into different lineages (e.g. osteogenic, chondrogenic and adipogenic) after suitable stimulation. (scirp.org)
  • Successful and reproducible adipogenic and osteogenic differentiation of MSCs in the device was demonstrated, verifying that an adequate long-term microfluidic cultivation environment was obtained. (rsc.org)
  • Parathyroid Hormone Secretion and Receptor Expression Determine the Age-Related Degree of Osteogenic Differentiation in Dental Pulp Stem Cells. (bvsalud.org)
  • To demonstrate the levels of parathyroid hormone secretion and genetic expressions of parathyroid hormone (PTH) and PTH1 receptor (PTH1R) genes in the dental pulp stem cells (DPSCs) from different age groups before and after induction of osteogenic differentiation. (bvsalud.org)
  • In addition, we also wanted to check their correlation with the degree of osteogenic differentiation. (bvsalud.org)
  • DPSCs were subjected to osteogenic differentiation and functional staining . (bvsalud.org)
  • After osteogenic induction, SHEDs were superior in the secretion of OPG, and the secretions of ALPL and PTH and the number of PTH1R positive cells were relatively low in the oDPSCs. (bvsalud.org)
  • This study emphasizes the criterion of aging , which affects the secretion of PTH by these cells , which in turn attenuates their osteogenic potential. (bvsalud.org)
  • In this work, for the first time, we adopt a multidisciplinary approach to characterize the morphological, biochemical, and molecular changes underlying the response of human bone marrow stromal cells to long-term simulated microgravity exposure during osteogenic differentiation. (lu.se)
  • Our results show that osteogenic differentiation is reduced while energy metabolism is promoted. (lu.se)
  • The investigation of cell proteome highlighted how simulated microgravity affects a relatively low number of proteins compared to time and/or osteogenic factors and has allowed us to reconstruct a hypothetical pipeline for cell response to simulated microgravity. (lu.se)
  • Activation of mTOR-dependent pathways regulates the specification and differentiation of CD4 + T effector cell subsets. (jci.org)
  • It is usually considered that the differentiation process of MSCs depends on specialized external stimulating factors, including cell signaling pathways, cytokines, and other physical stimuli. (wjgnet.com)
  • Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. (tocris.com)
  • Methods Pathways governing airway mucus cell differentiation include SRY (sex determining region Y)-box 2 (SOX2), Notch, forkhead box A3(FOXA3)/SAM pointed domain containing ETS transcription factor (SPDEF), epidermal growth factor (EGF) and the EGF-related neuregulins NRG1α and NRG1β. (bmj.com)
  • In this work, pathways governing mucus cell differentiation were investigated in lung tissues from patients with IPF and other chronic pulmonary disorders. (bmj.com)
  • ES cells have been an invaluable resource for advancing fundamental understanding of global transcriptional and epigenetic regulations, signaling pathways, and noncoding RNA in mammalian systems. (utexas.edu)
  • In the current study, we investigated the effect of prenatal exposure to DES on thymocyte differentiation involving apoptotic pathways. (aspetjournals.org)
  • The successful generation of beta cells in vitro and regeneration of pancreatic beta cells in vivo will require a thorough understanding of the pathways that direct the normal development and function of these cells. (lu.se)
  • We found novel proteins were dysregulated under simulated microgravity, including CSC1-like protein, involved in the mechanotransduction of pressure signals, and PTPN11, SLC44A1 and MME which are involved in osteoblast differentiation pathways and which may become the focus of future translational projects. (lu.se)
  • Our approach is to first understand how hESC interact with extracellular matrix materials by analyzing candidate adhesive substrate proteins and integrin receptors that mediate attachment, survival, proliferation and differentiation. (ca.gov)
  • However, details regarding the regulation of MSC/MPC proliferation and differentiation status are still unclear and it is likely that regulation involves both biological and mechanical influences in the different environments. (scirp.org)
  • An MC3T3-E1-based microarray dataset (accessioned as GSE46400) downloaded from the Gene Expression Omnibus included MC3T3-E1 cells with or without 14-day osteoblast differentiation osteoblast induction. (frontiersin.org)
  • Runx2 belongs to the Run family and is an essential upstream transcription factor during osteoblast differentiation. (frontiersin.org)
  • Osterix (OSX) is a transcription factor in the SP family, which is essential for osteoblast differentiation, and its expression is closely related to Runx2 ( 9 ). (frontiersin.org)
  • Over the last decades, mesenchymal stem cells (MSCs) have attracted extensive attention in the field of bone regeneration [ 4 - 7 ]. (hindawi.com)
  • MSCs are a population of nonhematopoietic adult stem cells that have the property of self-renewal and can differentiate into multiple lineages [ 8 - 10 ]. (hindawi.com)
  • With the in-depth researches of MSCs, an increasing number of studies have indicated that the therapeutic effects of MSCs can be attributed not only to their differentiation capacity but also to their paracrine action [ 21 ]. (hindawi.com)
  • Mesenchymal stem cells (MSCs), distributed in many tissues in the human body, are multipotent cells capable of differentiating in specific directions. (wjgnet.com)
  • Recent findings have revealed other underrated roles in the differentiation process of MSCs, such as material morphology and exosomes. (wjgnet.com)
  • Mesenchymal Stem Cells (MSCs) are a heterogeneous subset of stromal stem cells that originate from the mesoderm layer of the fetus. (tocris.com)
  • Osteoblast mainly originates from mesenchymal stem cells (MSCs), and it is involved in bone formation, remodeling, and reconstruction via the formation of a bone matrix and regulation of bone resorption ( 6 ). (frontiersin.org)
  • This study demonstrates the use of a microfluidic platform for long-term cultivation (3 weeks) of human mesenchymal stem-like cells (MSCs), a cell population of high interest for tissue engineering. (rsc.org)
  • The typical high motility of the MSCs required a strategy for preventing cells from inhabiting the feeding channels and thus interfere with a steady perfusion of medium to the cell cultivation chamber. (rsc.org)
  • There is a critical need for new technologies to facilitate growth and differentiation of human embryonic stem cells (hESC) using clinically acceptable, animal-free reagents. (ca.gov)
  • Differentiate human pluripotent stem cells (hPSCs) to cells of the endoderm lineage with our specialized cell culture media. (stemcell.com)
  • Differentiation of human and murine induced pluripotent stem cells to microglia-like cells. (alzforum.org)
  • Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids. (tocris.com)
  • In previous studies, induced pluripotent stem cells (iPSCs) differentiated into imDCs, and sinomenine (SN) was used to inhibit the maturation of imDCs. (newswise.com)
  • This study focused on exploring sinomenine (SN) to promote differentiation of induced pluripotent stem cells (iPSCs) into imDCs (SN-iPSCs-imDCs), and found SN-iPSCs-imDCs with worse donor-specific T-cell stimulatory function, and higher regulatory T-cell proliferative function in vitro and in vivo to induce high immune tolerance. (newswise.com)
  • Mouse embryonic stem (ES) cells are pluripotent cells, meaning that they can give rise to all tissues in the body. (utexas.edu)
  • Finally, we used this approach to encapsulate human Neural Stem Cells (hNSC) derived from human Induced Pluripotent Stem Cells (hIPSC), which were further differentiated into neurons within the capsules with negligible loss of viability. (rsc.org)
  • To ensure researchers have success working with our human induced pluripotent stem cell lines in their lab, our Angel Nelson highlights nuanced techniques and helpful tips while demonstrating our cardiac differentiation protocol for the WTC parental line and our gene-edited cells. (alleninstitute.org)
  • Generating specific cell lineages from induced pluripotent stem cells and embryonic stem cells is the holy grail of regenerative medicine. (shinshu-u.ac.jp)
  • By demonstrating the generation of cardiomyocytes from murine pluripotent stem cells, their concept shows promise as a regenerative therapy. (shinshu-u.ac.jp)
  • An anti-nucleolin DNA aptamer, iSN04, regulates cardiomyogenesis of murine pluripotent stem cells. (shinshu-u.ac.jp)
  • Self-renewal and pluripotency-the capacity to form any cell lineage-are inherent characteristics of induced pluripotent stem cells (iPSCs). (shinshu-u.ac.jp)
  • We had previously found that iSN04 promoted myogenic precursor cells (myoblasts) to differentiate into skeletal muscle cells and had hypothesized that the aptamer also enhanced differentiation of pluripotent stem cells. (shinshu-u.ac.jp)
  • However, when these pluripotent stem cells were treated after experiencing differentiating conditions for five days, specific marker genes were upregulated, and the cells committed to forming beating cardiomyocytes. (shinshu-u.ac.jp)
  • Differentiation of pluripotent embryonic stem cells into cardiomyocytes. (bvsalud.org)
  • Furthermore, mechanical stretch may induce the differentiation of BMSCs into mature osteoblasts and enhance the deposition of the bone matrix [ 18 - 20 ]. (hindawi.com)
  • Kit-derived Mature Mouse Dendritic Cells Induce Proliferation of Allogeneic T Cells. (rndsystems.com)
  • These results revealed that NDRG1 is functional in prostate cancer cells and able to induce expression of differentiation factors through p53 independent pathway. (scirp.org)
  • An odontogenic medium modified by the addition of recombinant human bone morphogenetic protein 2 was used in the experimental groups to induce differentiation. (scielo.br)
  • Consistent with previous observations of repressive role of Rbpj, Rbpj serves as a repressor of ES cell core factors in the absence of Notch signaling pathway. (utexas.edu)
  • Second, it results in the modulation of the Wnt signaling pathway that governs cell differentiation. (shinshu-u.ac.jp)
  • Most of these paracrine secretions include soluble factors and exosomes, which regulate the repair and regeneration processes at sites of damage by affecting cell proliferation, migration, and differentiation [ 22 , 23 ]. (hindawi.com)
  • T. Napso, N. Azzam, A. Lerner and F. Fares, " N-myc Downstream Regulated Gene 1 Increases Differentiation Factors Level in Human Prostate Cancer Cells without Affecting Cell Proliferation and Cell Cycle Profiles," Journal of Cancer Therapy , Vol. 4 No. 2, 2013, pp. 568-574. (scirp.org)
  • We have examined NGF-induced signal transduction events and neuronal differentiation in MAH cells, a neuronal progenitor cell line, in which the expression of the two NGF receptors, p140^(trk) (Trk) and p75^(LNGFR) (p75), has been independently manipulated. (caltech.edu)
  • These data provide evidence that p75 can modulate signal transduction through Trk in a neuronal progenitor cell context and that such modulation has functional consequences for the neuronal differentiation pathway induced by NGF. (caltech.edu)
  • This work will develop new cell lines for cultivated seafood production, provide insights into the relative merits of embryonic and adult stem cells as starting materials, and improve our ability to control the myogenic and adipogenic differentiation processes. (gfi.org)
  • Dr. Goswami, principal scientist at the Indian Council of Agricultural Research, is studying in vitro differentiation of carp embryonic, myogenic, and adipogenic stem cells in collaboration with the Ovissipour Lab at Virginia Tech. (gfi.org)
  • The effects of iPSCs-imDCs and SN-iPSCs-imDCs on T-cell stimulatory function, and regulatory T (Treg) cell proliferative function in vitro were analyzed by mixed lymphocyte reaction. (newswise.com)
  • Neuregulin1α, which drives mucus cell differentiation in vitro, was expressed in normal airway submucosal glands and in lungs from patients with IPF. (bmj.com)
  • The odontogenic differentiation of human dental pulp stem cells on nanofibrous poly(L-lactic acid) scaffolds in vitro and in vivo. (scielo.br)
  • This study was designed to determine the in vivo performance of three different materials as scaffolds for dental pulp stem cells (DPSC) undergoing induced odontogenic differentiation. (scielo.br)
  • Dental pulp stem cells (DPSC) seem to be the most appropriate cell group for the regenerative approach. (scielo.br)
  • Dental pulp stem cells: state of the art and suggestions for a true translation of research into therapy. (scielo.br)
  • Odontogenic capability: bone marrow stromal stem cells versus dental pulp stem cells. (scielo.br)
  • The therapeutic quality of dental pulp stem cells is largely based on their ability to retain their stemness characteristics. (bvsalud.org)
  • It regulates the serum cholesterol level by influencing the level of cholesterol uptake into cells. (cdc.gov)
  • It regulates the receptor for cholesterol, takes it out of the blood stream and puts it into the cells. (cdc.gov)
  • The results of these analyses revealed that the differentially expressed exosomal miRNAs participate in multiple biological processes, such as gene expression, synthesis of biomolecules, cell development, differentiation, and signal transduction, among others. (hindawi.com)
  • Li, L. and Jiang, J. (2011) Regulatory Factors of Mesenchymal Stem Cell Migration into Injured Tissues and Their Signal Transduction Mechanisms. (scirp.org)
  • Beck's team further determined that the differentiation of PDC's may occur in the presence of monocytic or myeloid DC differentiation, or as a differentiation lineage possessing a predominant myoblast population. (news-medical.net)
  • They can differentiate into cells of the mesodermal lineage, such as adipocytes, and osteocytes. (tocris.com)
  • Differentiation is a complex process involving actions of ES cell core factors, lineage specific regulators, epigenetic modifications, and chromatin remodelers. (utexas.edu)
  • Is Adipocyte Differentiation the Default Lineage for Mesenchymal Stem/Progenitor Cells after Loss of Mechanical Loading? (scirp.org)
  • The origins and basis for these fat deposits are largely unknown, but there is a possibility that the altered mechanical and biological environments lead to dysregulation of MSC/MPC and progression to preferential differentiation towards the adipocyte lineage. (scirp.org)
  • Hart, D. (2014) Is Adipocyte Differentiation the Default Lineage for Mesenchymal Stem/Progenitor Cells after Loss of Mechanical Loading? (scirp.org)
  • an anti-nucleolin DNA aptamer, iSN04, can determine a cell's lineage during differentiation. (shinshu-u.ac.jp)
  • iSN04 treatment under differentiating conditions inhibited stem cell commitment to the cardiac lineage. (shinshu-u.ac.jp)
  • Ultimately, terminal cell differentiation commits to the cardiomyocyte lineage. (shinshu-u.ac.jp)
  • Even though its sequence along with its expressional pattern in cancer cell lines are evident, the functional aspects concerning cell proliferation, viability, differentiation and cell cycle regulation of NDRG1 remains vague. (scirp.org)
  • Strengths of the experimental protocol include ease of fabrication and maintenance (gravity driven), good cell performance (viability/differentiation), as well as the possibility of exposing the culture to heterogeneous laminar flow for experimental purposes. (rsc.org)
  • In addition, we examined whether these substrates will support differentiation of retinal pigmented epithelial cells, an important, clinically relevant cell type that is currently in clinical trials for eye disease. (ca.gov)
  • Lukas Mager, MD, PhD: "Alternative splicing in epithelial cells. (meduniwien.ac.at)
  • NRG1α was detected in serous cells within normal submucosal glands and in epithelial cells lining honeycombing areas in IPF, and was not detected in other patients. (bmj.com)
  • Conclusion Expression of SOX2 and MUC5B and lack of SPDEF in atypically differentiated cells of bronchiolised distal airspaces are consistent with abnormal programming of airway epithelial cells in IPF. (bmj.com)
  • Here we examine the success rate of methods and data for differentiation by testing the ability of nine computational methods (CellNet, GarNet, EBseq, AME, DREME, HOMER, KMAC, diffTF and DeepAccess) to discover and rank candidate factors for eight target cell types with known reprogramming solutions. (nature.com)
  • Among the chromatin accessibility methods, complex methods DeepAccess and diffTF have higher correlation with the ranked significance of transcription factor candidates within reprogramming protocols for differentiation. (nature.com)
  • Normalized area under rank recall curve values for all methods are available in Supplementary Tables 2 - 6 and for epigenomic marks of liver cells in Extended Source Data Fig. 3 . (nature.com)
  • A Novel Gene Which Is Up-Regulated during Colon Epithelial Cell Differentiation and Down-Regulated in Colorectal Neoplasms," Laboratory Investigation: A Journal of Technical Methods and Pathology, Vol. 77, No. 1, 1997, pp. 85-92. (scirp.org)
  • Learn about the Allen Institute for Cell Science and access more information detailing our scientific methods and standard operating procedures . (alleninstitute.org)
  • Here, we develop a full generative model for probabilistically reconstructing trees of cellular differentiation from single-cell RNA-seq data. (arxiv.org)
  • While this work is motivated by cellular differentiation, we derive a tractable model that provides flexible densities for any data (coupled with an appropriate noise model) that arise from continuous evolution along a latent nonparametric tree. (arxiv.org)
  • N-myc downstream regulated gene 1 (NDRG1), also known as differentiation related gene 1, was previously identified as an up - regulated gene upon cellular differentiation. (scirp.org)
  • By integrating technologies, approaches, models and data into a common standardized framework, the Allen Institute for Cell Science is creating dynamic, visual models of how genetic information is transformed into cellular behavior, and how the molecules and organelles within each cell interact and function as systems. (alleninstitute.org)
  • To understand the molecular and cellular mechanisms that control beta cell maturation and function in the embryonic and adult pancreas. (lu.se)
  • Consequently, PLDL and PDL seemed to be promising scaffold candidates for odontogenic regeneration at least as HA-TCP, when they were applied with the DPSC induced for odontogenic differentiation. (scielo.br)
  • Although different stem cells, morphogens and scaffolds can be combined to simulate natural odontogenic regeneration, it is crucial to determine which options most closely replicate the characteristics of human dental tissues. (scielo.br)
  • In this context, in the undifferentiated state, the entropy would be large since fewer constraints exist on the gene expression programmes of the cell. (lu.se)
  • In order to assess these predictions, we compute the Shannon entropy for time- resolved single-cell gene expression data in two different experimental set-ups of haematopoietic differentiation. (lu.se)
  • Thanks to technological advances, we can now profile gene expression across thousands or millions of individual cells in parallel. (arxiv.org)
  • 2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
  • Transcription factor over-expression is a proven method for reprogramming cells to a desired cell type for regenerative medicine and therapeutic discovery. (nature.com)
  • In both analysis types, all PDCs showed partial to moderate expression of the cell marker CD34 indicating premature PDCs. (news-medical.net)
  • In a mouse experimental arthritis model, NKT17 cells are increased as the disease progresses, while NKT1 numbers negatively correlates with disease severity, with this protective effect of NKT1 linked to their IFN-γ expression. (nih.gov)
  • Upon activation, immature dendritic cells mature and increase the expression of class II MHC and co-stimulatory molecules important for effective antigen presentation to naïve T cells. (rndsystems.com)
  • Immature dendritic cells have the phenotype of CD11c + , with intermediate expression of B7-2/CD86, MHC class II, and little to no CD40. (rndsystems.com)
  • Mature dendritic cells have the phenotype of CD11c + , with increased expression of B7-2/CD86, MHC class II, and CD40. (rndsystems.com)
  • Until recently, transcriptomics was limited to bulk RNA sequencing, obscuring the underlying expression patterns of individual cells in favor of a global average. (arxiv.org)
  • MiR-211-5p upregulation by its mimic increased Arhgap11a expression in MC3T3-E1 cells. (frontiersin.org)
  • Activation of abnormal respiratory epithelial differentiation programs may contribute to the expression of MUC5B and bronchiolisation of the distal lung, a salient feature of IPF. (bmj.com)
  • Furthermore, human cDNA of NDRG1 from normal placenta was cloned into a eukaryotic expression vector and transfected into the three cancer cell lines. (scirp.org)
  • This resulted in over-expression of NDRG1, which in turn markedly up regulated two differentiation markers of the prostate tissue, p21 and cytokeratin 8/18. (scirp.org)
  • Unpredictably, cell cycle progression, cell proliferation and DNA synthesis were unaffected fol lowing NDRG1 expression. (scirp.org)
  • N. van Belzen, W. N. Dinjens, B. H. Eussen and F. T. Bosman, "Expression of Differentiation-Related Genes in Colorectal Cancer: Possible Implications for Prognosis," Histology and Histopathology, Vol. 13, No. 4, 1998, pp. 1233-1242. (scirp.org)
  • Draining lymph node CD4+ cells showed significantly increased miRNA 210 expression following a single exposure to TDI. (cdc.gov)
  • Hart, D.A. (2014) Why Mesenchymal Stem Cell/Progenitor Cell Heterogeneity in Specific Environments? (scirp.org)
  • Here, we utilized microarrayed patterns to provide a controlled biochemical and biomechanical microenvironment for mouse liver progenitor cell differentiation. (elifesciences.org)
  • IL-2 is secreted by T-cells upon stimulation of their T-cell receptor (TCR). (weizmann.ac.il)
  • Erythropoietin is a glycoprotein hormone that stimulates red blood cell (RBC) production in bone marrow via binding to the cell-surface receptor on hematopoietic progenitor cells, and it has been widely used for treating anemia [ 13 ]. (hindawi.com)
  • When DES-induced transcription factors were analyzed, estrogen receptor element (ERE), nuclear factor κB (NF-κB), nuclear factor of activated T cells (NF-AT), and activator protein-1 motifs on the Fas promoter, as well as ERE, NF-κB, and NF-AT motifs on the FasL promoter, showed binding affinity with the transcription factors. (aspetjournals.org)
  • [ 2 ] Options for second-line therapy in patients with relapsed/refractory disease include chemotherapy-free regimens with biologic targeted agents such as covalent Bruton tyrosine kinase (BTK) inhibitors, lenalidomide,venetoclax, and chimeric antigen receptor (CAR) T-cell therapy. (medscape.com)
  • Recently, a number of studies have confirmed that ADSCs possess the ability to differentiate into adipocytes, osteoblasts, and chondrocytes [ 16 - 18 ], suggesting that a broader source of stem cells is available for application in tissue engineering. (hindawi.com)
  • Alkaline phosphatase (ALP), osteocalcin (OCN), osteopontin (OPN), and runt-related transcription factor 2 (Runx2) are commonly used to evaluate differentiation and maturation of osteoblasts. (frontiersin.org)
  • Nevertheless, many intriguing questions remain to be answered regarding the regulation of Th17-mediated responses as well as their interactions with the other T-cell subsets. (nih.gov)
  • To examine the mechanism underlying DES-mediated regulation of Fas and FasL, we performed luciferase assays using T cells transfected with luciferase reporter constructs containing full-length Fas or FasL promoters. (aspetjournals.org)
  • Upon activation, naïve CD4+ T-cells can differentiate into one out of few possible lineages, each invoking a specific immune response. (weizmann.ac.il)
  • To directly differentiate to pancreatic cells, use our pancreatic progenitor kit to generate functional PDX1 + /NKX6.1 + cells that can be matured into insulin-producing beta cells. (stemcell.com)
  • Why Differentiate Dendritic Cells ex vivo ? (rndsystems.com)
  • The progenitor cells of the developing liver can differentiate toward both hepatocyte and biliary cell fates. (elifesciences.org)
  • At the Stem Cell Differentiation and Cytogenetics Group, we are interested in the effects of ionizing radiation on organs such as the brain, the heart, and the lung trying to elucidate the risks for patients treated with ionizing radiation as well as for astronauts, who are inevitably exposed to ionizing radiation on their missions into space. (gsi.de)
  • This is an important, unmet need in the field and development of such a substrate would aid in moving stem cell therapies to the clinic. (ca.gov)
  • Among these, adipose-derived stem cells (ADSCs) are a type of mesenchymal stem cell isolated from adipose tissue, which has the advantages of abundant storage in vivo , easy acquisition, and expansion [ 13 - 15 ]. (hindawi.com)
  • The metaphor of a potential epigenetic differentiation landscape broadly suggests that during differentiation a stem cell approaches a stable equili- brium state from a higher free energy towards a stable equilibrium state which represents the final cell type. (lu.se)
  • Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. (tocris.com)
  • This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. (tocris.com)
  • Our guide highlights the use of small molecules in stem cell research and cell therapy and lists relevant products. (tocris.com)
  • Our Stem Cells review gives an overview of the use of small molecules in the control of stem cell growth & differentiation and somatic cell reprogramming. (tocris.com)
  • Developed in-house, this poster summarizes how small molecules have been used in protocols across the stem cell workflow. (tocris.com)
  • Wu, Y. and Zhao, R.C. (2012) The Role of Chemokines in Mesenchymal Stem Cell Homing to the Myocardium. (scirp.org)
  • Stem Cell Reviews, 8, 243-250. (scirp.org)
  • Fehrer, C. and Lepperdinger, G. (2005) Mesenchymal Stem Cell Aging. (scirp.org)
  • Mesenchymal stem cell medium was used in the control groups. (scielo.br)
  • Aptamers are single-stranded pieces of DNA that bind to target proteins and are able to modulate signaling cascades during cell differentiation when a stem cell commits to a specific functional role or phenotype. (shinshu-u.ac.jp)
  • Treatment selection takes into account patient age, fitness, and whether autologous stem cell transplantation (ASCT) is planned. (medscape.com)
  • old DPSCs (oDPSCs), 60+ years old) were characterized for mesenchymal stem cell (MSC) markers. (bvsalud.org)
  • All SHEDs, yDPSCs, and oDPSCs were found to be expressing mesenchymal stem cell markers. (bvsalud.org)
  • For this purpose, we use human stem cells that play a pivotal role in tissue development, maintenance and function. (gsi.de)
  • Ando, W., Heard, B., Nakamura, N., Frank, C.B. and Hart, D.A. (2012) Ovine Synovial Membrane-Derived Mescenchymal Progenitor Cells Retain the Phenotype of the Original Tissue That Was Exposed to in Vivo Inflammation: Evidence for a Suppressed Chondrogenic Differentiation Potential of the Cells. (scirp.org)
  • Hard tissue formation in a porous HA/TCP ceramic scaffold loaded with stromal cells derived from dental pulp and bone marrow. (scielo.br)
  • Mesenchymal cells derived from the dermomyotome condense within connective tissue (mesenchymal) scaffolds to form 2 common muscle masses. (medscape.com)
  • First, we characterized the cell surface receptors for matrix proteins on stem cells and identified specific proteins that will support adhesion and growth of the cells. (ca.gov)
  • Second, using an interdisciplinary approach, we developed a novel method to screen peptides from these proteins for their ability to support adhesion and proliferation of the stem cells. (ca.gov)
  • Based on our characterization of cell surface receptors for matrix proteins on stem cells completed in the first year, we went on to test specific, purified proteins that will support adhesion and growth of the cells. (ca.gov)
  • By transporting "cargos" such as proteins, RNAs, DNAs, and lipids [ 26 ], exosomes regulate the eventual fate of recipient cells. (hindawi.com)
  • This kit contains the following optimized proteins and reagents to drive the efficient differentiation of mouse monocytes into dendritic cells. (rndsystems.com)
  • There was shift in mobility of probes (ERE or NF-κB2) of both Fas and FasL in the presence of nuclear proteins from DES-treated cells, and the shift was specific to DES because these probes failed to shift their mobility in the presence of nuclear proteins from vehicle-treated cells. (aspetjournals.org)
  • Bone morphogenetic proteins (BMP), known as active components of odontogenic differentiation and tooth development, have been used as a growth factor in an appropriate medium. (scielo.br)
  • Particularly, human embryonic stem cells (hES cells), which can give rise to all tissues of an organism (Figure A), are used to generate mature cells of the heart (Figure B) allowing us to study radiation/microgravity-induced cardiovascular alterations, which may occur during long-term space missions. (gsi.de)
  • Stem cells have potential as a source of cells and tissues for research and treatment of disease. (tocris.com)
  • Mesenchymal stem/progenitor cells (MSC/MPC) are found in many tissues and fluids including bone marrow, adipose tissues, muscle, synovial membranes, synovial fluid, and blood. (scirp.org)
  • The pituitary gland produces thyroid stimulating hormone (TSH) that goes into the blood stream to activate thyroid cells, which then secrete T3 and T4 into the peripheral tissues. (cdc.gov)
  • There has been much progress in the past year, leading to identification of the molecular mechanisms that drive differentiation of Th17 T cells. (nih.gov)
  • hES cells also serve as a basis for cerebral organoid models (Figure C) to better understand e.g. the mechanisms of cognitive impairment and radiation necrosis as severe side effects of radiation therapies. (gsi.de)
  • The authors sought to identify mechanisms driving the differentiation of mucus cells during the bronchiolisation process. (bmj.com)
  • However, the molecular mechanisms of how ES cells are differentiated remain much less understood. (utexas.edu)
  • Understanding the mechanisms underlying embryonic beta cell development will be a prerequisite for the development of a universally available cell replacement therapy in diabetes. (lu.se)
  • Impact Understanding the mechanisms underlying beta cell development and function will be a prerequisite for cell replacement therapy in diabetes. (lu.se)
  • Our signature-based screen established several categories of genes based on their comparative functions during the differentiation of ES cells. (utexas.edu)
  • K. Kokame, H. Kato and T. Miyata, "Homocysteine-Respondent Genes in Vascular Endothelial Cells Identified by Differential Display Analysis. (scirp.org)
  • This decision is influenced by the spectrum of cytokines that the cells sense. (weizmann.ac.il)
  • The differentiating cells also actively alter these signals by producing relevant cytokines, thus influencing the differentiation process through extracellular feedbacks. (weizmann.ac.il)
  • While differentiation is usually studied under polarizing conditions, applying only one signal at a time, cells in vivo are expected to sense a more complex environment, in which they may be simultaneously exposed to combinations of cytokines. (weizmann.ac.il)
  • We explore differentiation under mixed input conditions by exposing cells to varied combinations of cytokines, experimentally mapping the "decision space" of the differentiation process at the single cell level. (weizmann.ac.il)
  • Cytokines produced by DCs can also promote the differentiation of CD4 + T helper cells as part of immune activation. (rndsystems.com)
  • Both categories of cells resulting from atypical PDC differentiation are thought to represent two ends of a spectrum of maturation. (news-medical.net)
  • However, these cells have limitations, such as rapid maturation and a short lifespan in vivo . (newswise.com)
  • See More Use our definitive endoderm (DE) differentiation kit to obtain functional DE cells that are capable of downstream differentiation to hepatic, intestinal, pancreatic and pulmonary cells. (stemcell.com)
  • Therefore, a central goal of diabetes research is to regenerate pancreatic beta cells or produce large numbers of functional beta cells for transplantation into diabetic patients. (lu.se)
  • In this work, to elucidate the functional role of miRNA 210 during TDI sensitization, BALB/c mice were dermally exposed to TDI (4% v/v) or vehicle and endpoints were evaluated via RT-PCR, magnetic cell separation, and flow cytometry. (cdc.gov)
  • This data indicates that miRNA 210 has a functional role in TDI sensitization which may be related to regulatory T cell differentiation and function. (cdc.gov)
  • However, the pathway downstream NDRG1, involving p21 and c8/18, regulating cell cycle progression and DNA synthesis is unfunctional. (scirp.org)
  • Extracellular feedback occurs when a cell expresses receptors to a cytokine that it secretes. (weizmann.ac.il)
  • We study extracellular feedback by the cytokine IL-2 in the context of T-cell activation. (weizmann.ac.il)
  • We proposed to develop novel, well-defined, synthetic extracellular matrices that support survival and proliferation of human embryonic stem cells. (ca.gov)
  • We propose to develop novel, well-defined, synthetic extracellular matrices for growth and differentiation of hESC. (ca.gov)
  • We present here a microfluidic device that generates sub-millimetric hollow hydrogel spheres, encapsulating cells and coated internally with a layer of reconstituted extracellular matrix (ECM) of a few microns thick. (rsc.org)
  • This project aims to develop continuous cell lines from carp and characterize their adipogenic and myogenic differentiation. (gfi.org)
  • Our data therefore suggest that TCR signal strength during thymic differentiation may influence not only IFN-γ production, but also the protective function of iNKT cells in arthritis. (nih.gov)
  • Finally, using primary lung stem cells or lymphocytes from different donors, we study the effects of radon on patients, who are treated in radon spas or baths to alleviate rheumatic diseases. (gsi.de)
  • however, due to constitutive mTORC1 activation, these cells retained a terminally differentiated effector phenotype and were incapable of transitioning into a memory state. (jci.org)
  • These findings support a model by which decision under mixed conditions follows a fuzzy logic, where cells project their internal state onto the output phenotype stochastically, with probabilities which depend on the system's input. (weizmann.ac.il)
  • Various subsets of invariant natural killer T (iNKT) cells with different cytokine productions develop in the mouse thymus, but the factors driving their differentiation remain unclear. (nih.gov)
  • The effects of erythropoietin (EPO) on the behaviors of bone marrow mesenchymal stem cells (BMSCs) subjected to mechanical stretch remain unclear. (hindawi.com)
  • A) Colonies of human embryonic stem cells, bright field microscopy, scale bar: 100µm. (gsi.de)
  • D) Karyotype of a human cell after heavy ion exposure revealed by mFISH technique. (gsi.de)
  • Finally, we identified a cyclic RGD peptide that supports growth of human embryonic stem cells. (ca.gov)
  • Opened or reconstituted Mouse Dendritic Cell Base Media, Recombinant Mouse GM-CSF, Recombinant Mouse IL-4, or Recombinant Human TNF-alpha should be stored at 2-8 ° C under sterile conditions for up to 30 days or at -20 ° C to -70 ° C in a manual defrost freezer for up to 3 months. (rndsystems.com)
  • The molecular study of fat cell development in the human body is essential for our understanding of obesity and related diseases. (mdpi.com)
  • Gimble, J.M., Bunnell, B.A. and Guilak, F. (2012) Human Adipose-Derived Cells: An Update on the Transition to Clinical Translation. (scirp.org)
  • editors Culture of human stem cells. (scielo.br)
  • 5. In 2001, France and Germany requested the United Nations General Assembly to develop international conventions on human reproductive cloning, therapeutic cloning and research on stem cells. (who.int)
  • Unfortunately, regenerative medicine is not yet feasible outside a laboratory setting as available protocols to generate target cells are complicated and expensive. (shinshu-u.ac.jp)
  • To use this knowledge to improve beta cell differentiation protocols. (lu.se)
  • The group analyzed seven MN patients who presented with PDC proliferations comprising 5-26% of that blood cell or bone marrow. (news-medical.net)
  • Borlongan, C.V., Glover, L.E., Tajiri, N., Kaneko, Y. and Freeman, T.B. (2011) The Great Migration of Bone Marrow-Derived Stem Cells towards the Ischemic Brain: Therapeutic Implications for Stroke and Other Neurological Disorders. (scirp.org)
  • 2012). In an additional work, performed in collaboration with Yuval Hart and Uri Alon from the department of molecular cell biology, we show that IL-2 pleiotropic activity can result in homeostasis of cell numbers, where the final cell concentration is independent of initial concentration over a large range (Hart et. (weizmann.ac.il)
  • Dr. Goswami has a strong background in molecular biology and experience in the development and characterization of fish cell lines. (gfi.org)
  • Using various assays like RNA sequencing, cell staining and imaging, and molecular interaction and pathway analysis, the researchers investigated iSN04's effect on murine ESCs and iPSCs. (shinshu-u.ac.jp)
  • The fluorochrome-labeled cells are analyzed by flow cytometry, which categorizes individual cells according to size, granularity, fluorochrome, and fluorochrome intensity. (cdc.gov)
  • This has catalyzed research in both early embryogenesis as a model system for mammalian development as well as regenerative medicine as a renewable source of unspecialized cells which can be converted into nearly any cell type required by a patient. (utexas.edu)