• In 2017, the research team used the Tianzhou-1 cargo spacecraft to conduct research on the proliferation and differentiation of embryonic stem cells on a mouse. (pdnews.cn)
  • Furthermore, the study examined the effects of cooperating oncoproteins such as Ras or Bcr/Abl, which allowed for both factor-independent proliferation and differentiation, or Bcl-2, which permitted factor-independent survival but not proliferation. (nih.gov)
  • Interleukin 13: novel role in direct regulation of proliferation and differentiation of primitive hematopoietic progenitor cells. (ox.ac.uk)
  • The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCultâ„¢ ) when supplemented with appropriate cytokines. (stemcell.com)
  • Hematopoiesis, in particular the genetic control of hematopoietic cell proliferation and differentiation is also studied in human cell cultures and transgenic mouse model systems. (lu.se)
  • Another CFU, the colony-forming unit-spleen (CFU-S), was the basis of an in vivo clonal colony formation, which depends on the ability of infused bone marrow cells to give rise to clones of maturing hematopoietic cells in the spleens of irradiated mice after 8 to 12 days. (wikipedia.org)
  • Stem and progenitor cell commitment processes have been subject to intense experimental studies over the last decade with some emphasis on embryonic and hematopoietic stem cells. (lu.se)
  • In vertebrates, the very first definitive HSCs arise from the ventral endothelial wall of the embryonic aorta within the (midgestational) aorta-gonad-mesonephros region, through a process known as endothelial-to-hematopoietic transition. (wikipedia.org)
  • The very first hematopoietic stem cells during (mouse and human) embryonic development are found in aorta-gonad-mesonephros region and the vitelline and umbilical arteries. (wikipedia.org)
  • Among them is the first international research on human pluripotent stem cells in vitro hematopoietic differentiation of human embryonic stem cells under space conditions. (pdnews.cn)
  • From the experimental results, China has successfully achieved the first hematopoietic differentiation of human embryonic stem cells under space conditions, accomplishing its first experimental goal. (pdnews.cn)
  • All-trans retinoic acid (RA) is the most active metabolite of vitamin A. Several studies have described a pivotal role for RA signalling in different biological processes such as cell growth and differentiation, embryonic development and organogenesis. (mdpi.com)
  • Comparison of toxicity of benzene metabolite hydroquinone in hematopoietic stem cells derived from murine embryonic yolk sac and adult bone marrow. (cdc.gov)
  • In the current study, we compared the effects of hydroquinone (HQ), a major metabolite of benzene in humans and animals, on mouse embryonic yolk sac hematopoietic stem cells (YS-HSCs) and adult bone marrow hematopoietic stem cells (BM-HSCs). (cdc.gov)
  • The study established an experimental system for comparison of the hematopoietic toxicity and leukemogenicity of benzene and metabolites during mouse embryonic development and adulthood. (cdc.gov)
  • A potassium inward rectifier (K(it)) current was previously shown by us to be induced in primitive hematopoietic progenitor cells, stimulated with the combination of interleukin-3 (IL-3) and stem cell factor (SCF). (bgu.ac.il)
  • The variable expression of two essential inward rectifying potassium channels early in the course of hematopoietic progenitor cell differentiation may play a potentially important role in potassium homeostasis in these cells. (bgu.ac.il)
  • The hematopoietic tissue contains cells with long-term and short-term regeneration capacities and committed multipotent, oligopotent, and unipotent progenitors. (wikipedia.org)
  • Using Drosophila hematopoietic organ lymph gland, we demonstrate that Fatty Acid Oxidation (FAO) is essential for the differentiation of blood cell progenitors. (bvsalud.org)
  • We examined hematopoietic stem cells at four stages of differentiation, lineage-committed progenitors, and mature myeloid and lymphoid cells. (nih.gov)
  • Loss of hematopoietic stem cell function was associated with decreased expression of Cdkn1a (encoding the cell cycle inhibitor p21(cdk)), Sfpi1, Hoxb4 and Bmi1 (encoding the transcription factors PU.1, HoxB4 and Bmi-1, respectively) and altered integrin expression in Lin(-)Sca-1(+)c-Kit(+) cells, whereas PU.1 was upregulated in erythroid progenitors. (ox.ac.uk)
  • Recently we have developed an experimental and analytical method, termed massively parallel single-cell RNA-Seq (MARS-Seq), for unbiased classification of individual cells from their native context and successfully applied it for characterization of immune and hematopoietic progenitors. (europa.eu)
  • Exciting preliminary result show that indeed our approach is starting to uncover the complexity of hematopoietic progenitors and the regulatory circuits driving hematopoietic decisions. (europa.eu)
  • We will pursue the following aims: (i) Generate a refined model of hematopoiesis by comprehensive single-cell RNA-Seq profiling of hematopoietic progenitors, (ii) validate the predicted model by in vivo functional developmental assays and then (iii) test candidate transcription and chromatin factors uncovered by our model for their role in controlling progression towards various lineages using single-cell measurements combined with CRISPR screens. (europa.eu)
  • In vertebrates, the vast majority of hematopoiesis occurs in the bone marrow and is derived from a limited number of hematopoietic stem cells that are multipotent and capable of extensive self-renewal. (wikipedia.org)
  • Lei said that in the next phase, the research team needs to conduct comprehensive detection and analysis of this group of hematopoietic stem cells, and screen out the relevant genes affecting the early hematopoiesis differentiation of human pluripotent stem cells in the space environment by comparing with the ground control group. (pdnews.cn)
  • Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. (nih.gov)
  • Wnt signaling increases hematopoietic stem cell self-renewal and is activated in both myeloid and lymphoid malignancies, indicating involvement in both normal and malignant hematopoiesis. (ox.ac.uk)
  • Bone marrow-derived hematopoietic and mesenchymal stem cells as targets for Histoplasma capulatum infection: implications for hematopoiesis, differentiation, and proliferation. (urosario.edu.co)
  • To determine whether the interaction of hematopoietic stem cells (HSCs) and bone marrow-derived mesenchymal stem cells (BMMSCs) with Histoplasma capsulatum affects hematopoiesis, differentiation and proliferation of these precursor cells. (urosario.edu.co)
  • BMMSCs) with Histoplasma capsulatum affects hematopoiesis, differentiation and proliferation of these precursor cells. (urosario.edu.co)
  • Hematopoiesis is an important model for stem cell differentiation with great medical significance. (europa.eu)
  • Together, our study is expected to generate a revised and high-resolution hematopoietic model and decipher the regulatory networks that control hematopoiesis. (europa.eu)
  • Hematopoietic stem cells (HSCs) are the stem cells that give rise to other blood cells. (wikipedia.org)
  • Long-term hematopoietic stem cells (LT-HSCs) maintain hematopoietic output throughout an animal's lifespan. (biorxiv.org)
  • HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
  • HSCs specifically are defined by their ability for self-renewal and to reconstitute the entire hematopoietic system following transplantation. (stemcell.com)
  • It is believed that benzene and its metabolites target hematopoietic stem cells (HSCs) to cause toxicity and cancer in the hematopoietic system. (cdc.gov)
  • HQ reduced the proliferation and the differentiation and colony formation, but increased the apoptosis of both YS-HSCs and BM-HSCs. (cdc.gov)
  • Our research focuses on how hematopoietic stem cells (HSCs) and their immediate offspring are regulated in situations such as infections, stress, aging and leukemia. (lu.se)
  • We have developed and applied FACS-based high-resolution cell fractionation procedures to map the differentiation processes from HSCs. (lu.se)
  • A broader interest of our laboratory is to understand the molecular regulation of HSCs both from the perspectives of self-renewal and differentiation, and how these features become altered upon disease-causing or disease-associated perturbations. (lu.se)
  • We assayed telomerase activity in single cells of the hematopoietic and immune systems. (nih.gov)
  • GS-2 iPSCs showed the capacity to differentiate into cells of the hematopoietic lineage. (biomedcentral.com)
  • Hematopoietic stem/progenitor cells (HSPC) are known to be functionally diverse in terms of their self-renewal potential and lineage output. (lu.se)
  • Beck's team further determined that the differentiation of PDC's may occur in the presence of monocytic or myeloid DC differentiation, or as a differentiation lineage possessing a predominant myoblast population. (news-medical.net)
  • Lack of expression of lineage markers is used in combination with detection of several positive cell-surface markers to isolate hematopoietic stem cells. (wikipedia.org)
  • Heterogeneity within hematopoietic progenitor populations has considerably limited characterization and molecular understanding of lineage commitment in both health and disease. (europa.eu)
  • Also making up a part of the hematopoietic system are short-term repopulating progenitor cells, which give rise to lineage-specific cell types. (stemcell.com)
  • Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
  • Lineage promiscuity in hematopoietic differentiation and leukemia. (bdbiosciences.com)
  • The molecular pathways of normal myeloid differentiation, as well as the mechanisms by which oncogenes disrupt this process, remain poorly understood. (nih.gov)
  • Expression of MLL-fusions enhances proliferation and blocks myeloid differentiation of hematopoietic progenitor cells, leading to their pathological accumulation. (nature.com)
  • In this study, I have tried to address the role of the nucleolus in cellular differentiation. (nii.ac.jp)
  • These results suggest that the downregulation of rRNA transcription accelerates cellular differentiation at least in hematopoietic cells. (nii.ac.jp)
  • Our results indicate that DLVs represent recombinants between a common 'vector' related to a chicken endogenous virus and one of three types of cellular gene possibly involved in haematopoietic differentiation. (nature.com)
  • Our study thus reveals how the cellular signaling machinery integrates with the metabolic cue to facilitate the differentiation program. (bvsalud.org)
  • Well-illustrated and clinically focused, it details the basic science and clinical practice of hematology and hematopoietic cellular therapy-covering virtually all aspects of hematology in one definitive resource. (elsevierhealth.com)
  • Hematopoietic Stem Cell and Leukaemic Stem Cell environmental stress responses (In vitro biomimicry of hypoxia, normoxia, hypothermia, hyperthermia, hypoglycemia and hyperglycemia): Effect on cellular growth, cell cycle distribution, apoptosis and cellular metabolism. (imperial.ac.uk)
  • It is used both by hematopoietic researchers and clinical labs to assess the potency of transplantation units of cord blood (CB) and hematopoietic cellular therapy products. (stemcell.com)
  • Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
  • Studies on the pathogenesis of bone marrow failure syndromes have helped to elucidate the regulation of cellular growth and differentiation in the hematopoietic environment. (medscape.com)
  • ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)
  • Consequently, investigation of molecular heterogeneity in the differentiation potential of HSPC is vital to identify novel regulators that affect generation of specific cell types, especially RBC. (lu.se)
  • One sentence summary Murine hematopoietic stem cells display transcriptional heterogeneity that is quantitatively altered with age and leads to the age-dependent myeloid bias evident after inflammatory challenge. (biorxiv.org)
  • A reduction of GATA-2 expression or activity is required for the differentiation of precursors to hematopoietic cells. (elsevierpure.com)
  • Leukemic myoblast differentiation into PDCs occurs as a result of sharing a common hematopoietic precursor to dendritic cells, monocytes, and myeloid cells. (news-medical.net)
  • These results demonstrated that GATA-2 is expressed in the precursor of hematopoietic cells and works as a gatekeeper to preserve their immaturity. (elsevierpure.com)
  • erythroid cells after in vitro differentiation, despite having similar frequencies of phenotypic HSC initially. (lu.se)
  • In vitro differentiation of GS-2 iPSCs into hematopoietic stem and progenitor cells was done using Op9 feeder layers and specified media. (biomedcentral.com)
  • SETD2 loss synergizes with pharmacologic inhibition of the H3K79 methyltransferase DOT1L to induce DNA damage, growth arrest, differentiation, and apoptosis. (nature.com)
  • Constitutive activation of canonical Wnt signaling therefore causes multilineage differentiation block and compromised hematopoietic stem cell maintenance. (ox.ac.uk)
  • MYBBP1A attenuated transcriptional activity of c-Myc and promoted megakaryocytic differentiation. (nii.ac.jp)
  • In this review, we focus on the impact of ncRNA post-transcriptional regulatory mechanisms, especially those of microRNAs and lncRNAs, in RA signalling pathways during differentiation and disease. (mdpi.com)
  • Differentiation was normal and terminal as evidenced by morphology, cell surface markers, gene expression, and functional assays. (nih.gov)
  • Hematopoietic stem cells constitute 1:10,000 of cells in myeloid tissue. (wikipedia.org)
  • Experts said that the unique space microgravity environment may be a new way to maintain undifferentiated proliferation of stem cells, enhance the efficiency of induced differentiation, and improve the level of three-dimensional tissue construction. (pdnews.cn)
  • Differentiation of cells present in hematopoietic tissue. (studystack.com)
  • The use of ASCs in regenerative medicine and tissue engineering research has important advantages in comparison with ESCs, since there are no ethical complications and the process of differentiation of these cells is better controlled. (bvsalud.org)
  • This will provide key insights on how ubiquitin signaling plays a vital role in stem cell differentiation and how perturbations in these networks can lead to disorders such as cancer. (lu.se)
  • In the first evaluation experiment, expansion ability of T cells activated by Cluster of Differentiation (CD) CD3/CD28 magnetic beads is less than 5 times. (who.int)
  • I found that rRNA transcription was downregulated during hematopoietic differentiation in both human leukemia cell line and mouse hematopoietic stem cells. (nii.ac.jp)
  • A major limitation in approaching this problem has been the lack of suitable cell lines that exhibit normal, terminal, and synchronous differentiation in the absence of endogenous oncoproteins and in response to physiologic cytokines, and whose differentiation can be arrested by ectopically expressed human oncoproteins. (nih.gov)
  • Our methods and models may provide a platform for future medical advancements including a large-scale European collaborative project to discover a comprehensive human hematopoietic tree. (europa.eu)
  • An odontogenic medium modified by the addition of recombinant human bone morphogenetic protein 2 was used in the experimental groups to induce differentiation. (scielo.br)
  • The odontogenic differentiation of human dental pulp stem cells on nanofibrous poly(L-lactic acid) scaffolds in vitro and in vivo. (scielo.br)
  • 8,9 Because dental stem cells (DSCs) are easy to obtain and present a great potential of differentiation, there has been a growing interest in their use in regenerative medicine for treatment of various human diseases. (bvsalud.org)
  • The differentiation of the cells could be arrested by heterologous oncoproteins including AML1/ETO, PML/RARalpha, PLZF/RARalpha, Nup98/HoxA9, and other Hox proteins. (nih.gov)
  • Bone morphogenetic proteins (BMP), known as active components of odontogenic differentiation and tooth development, have been used as a growth factor in an appropriate medium. (scielo.br)
  • Since each ubiquitin ligase can recognize its target proteins through a specific degron, we are interested in delineating ubiquitin ligase-target protein interactions that are triggered by differentiation stimulus in stem cells. (lu.se)
  • Clones were identified that proliferated as immortalized blasts in the presence of estrogen, and that exhibited granulocytic, monocytic, or bipotential (granulocytic and monocytic) differentiation on estrogen withdrawal. (nih.gov)
  • Consequently, PLDL and PDL seemed to be promising scaffold candidates for odontogenic regeneration at least as HA-TCP, when they were applied with the DPSC induced for odontogenic differentiation. (scielo.br)
  • Is the Subject Area "Hematopoietic stem cell transplantation" applicable to this article? (plos.org)
  • The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)
  • Interestingly, acetate supplementation rescues both histone acetylation and the differentiation defects. (bvsalud.org)
  • In this study we investigated for the first time the potential role of IL-13 in the regulation of the growth of hematopoietic progenitor cells. (ox.ac.uk)
  • and positive regulation of cell differentiation. (mcw.edu)
  • The CD45 antigen, a marker of hematopoietic cells, was expressed in a small fraction of transgene (TG)-derived GFP + cells. (elsevierpure.com)
  • The remaining TG-GFP + /CD45 - cells were adherent to plastic and produced CD45 + hematopoietic cells abundantly when cultured in vitro. (elsevierpure.com)
  • Exogenous expression of GATA-2 in TG-GFP + /CD45 - cells from the AGM region inhibited their differentiation into CD45 + cells. (elsevierpure.com)
  • Six of the seven cases exhibited intermediate subpopulations, which evidence differentiation from a population of leukemic myeloblasts. (news-medical.net)
  • These myeloid cell lines provide tools for examining the biochemical and genetic pathways that accompany normal differentiation as well as a system in which to dissect how other leukemic oncoproteins interfere with these pathways. (nih.gov)
  • We report here activated canonical Wnt signaling in the hematopoietic system through conditional expression of a stable form of beta-catenin. (ox.ac.uk)
  • Physiologic aging in both humans and mice leads to permanent changes in LT-HSC function, such as myeloid-biased hematopoietic output ( Akunuru and Geiger, 2016 ). (biorxiv.org)
  • In response to the inhibition of rRNA transcription, nucleolar protein MYBBP1A was translocated from the nucleolus to the nucleoplasm and interacted with c-Myc, which is one of the master regulator of hematopoietic differentiation. (nii.ac.jp)
  • In early blood cells, called hematopoietic stem cells, the methylation patterns established by DNA methyltransferase 3 alpha promote maturation (differentiation) into different blood cell types. (medlineplus.gov)
  • As in cytogenetically normal acute myeloid leukemia (described above), the mutations disrupt the normal pattern of methylation in cells, which blocks differentiation. (medlineplus.gov)
  • These diseases are intrinsic disorders of the bone marrow involving disruption in the homeostasis and function of hematopoietic stem cells, resulting in inadequate production of either a single or multiple cell lines (erythroid for red cells, myeloid for white blood cells, megakaryocytic for platelets). (medscape.com)
  • Collectively, these are referred to as hematopoietic stem and progenitor cells (HSPCs). (stemcell.com)
  • Hematopoietic stem cells can replenish all blood cell types (i.e., are multipotent) and self-renew. (wikipedia.org)
  • The group concluded by stating that acute leukemia with PDC differentiation and typical cases of acute myeloid leukemia, NOS, or acute myelomonocytic leukemia are indistinguishable. (news-medical.net)
  • Researchers speculate that the altered gene activity prevents hematopoietic stem cells from differentiating normally, which leads to the overproduction of abnormal, immature white blood cells characteristic of acute myeloid leukemia. (medlineplus.gov)
  • By studying murine transgenic leukemia models developed in our laboratory, we are currently exploring the relationships between hematopoietic progenitor cell identities and the development and progression of acute leukemia. (lu.se)
  • These factors may regulate myeloid versus lymphoid balance with age, and can potentially mitigate the long-term deleterious effects of inflammation that lead to hematopoietic pathologies. (biorxiv.org)
  • This study was designed to determine the in vivo performance of three different materials as scaffolds for dental pulp stem cells (DPSC) undergoing induced odontogenic differentiation. (scielo.br)
  • The department focuses on research that aims to develop gene therapy for hematopoietic cells using viral vectors. (lu.se)