Differentiation hematopoiesis. Medical search. Frequent questions

Using previous findings in healthy cells as a reference, we analyzed scRNA-seq data obtained from patients with myelodysplastic syndrome (MDS) and detected specific alterations of the expression dynamics of genes involved in erythroid differentiation in all patients with MDS such as TRIB2. (cun.es)
HBS1L encodes a protein with apparent GTP binding activity and is included in different cellular processes while MYB encodes a transcription factor for erythroid differentiation in hematopoiesis [10]. (scirp.org)

A long-term multi-organ co-culture system coupled with quantitative imaging to visualize blood cell cycle progression, analyze polarized blood cell mitosis, and track blood cell differentiation kinetics during Drosophila hematopoiesis in homeostatic condition and following infection. (elifesciences.org)
Induced p53 deficiency, as well as overexpression of the anti-apoptotic proteins Bcl-2 and E1B-19K, significantly relieves the decreased blood cells numbers caused by zTERT knockdown, but not the impaired blood cell differentiation. (harvard.edu)
In contrast with this model, this study shows that genetic ablation of the PSC does not cause an increase in blood cell differentiation or a loss of blood cell progenitors. (sdbonline.org)
Further, premature blood cell differentiation when PSC specification or signaling was impaired, led to assigning the PSC a role equivalent to the vertebrate hematopoietic niche. (sdbonline.org)
The PSC contributes to lymph gland homeostasis by regulating blood cell differentiation, rather than by maintaining core progenitors. (sdbonline.org)

MYB is an essential regulator of primitive human hematopoiesis in human pluripotent stem cell differentiation cultures. (nih.gov)
Our data indicate that in contrast to its previously attributed exclusive role in definitive hematopoiesis, MYB is indispensable for primitive human hematopoiesis. (nih.gov)
Genetic studies have also suggested a role for Tropomyosin 1 ( TPM1 ) in human platelet trait variation [ 6 ], though no prior studies have elucidated if or how TPM1 impacts human hematopoiesis. (biomedcentral.com)

DNMT3A is a critical regulator of hematopoietic stem cell (HSC) differentiation and somatic DNMT3A mutations are frequent in hematologic malignancies and clonal hematopoiesis. (haematologica.org)
Although clonal hematopoiesis of indeterminate potential has been linked to a higher risk for myeloid neoplasms and inflammatory diseases, not much is known about the individual risk of developing blood-related cancers in patients 80 years of age and older. (medpagetoday.com)
Single-cell transcriptome analyses reveal distinct gene expression signatures of severe COVID-19 in the presence of clonal hematopoiesis. (cdc.gov)

The Paralkar Lab has identified that key hematopoietic and leukemic transcription factors bind to rDNA and regulate rRNA transcription, and we are interested in understanding how the binding of cell-type-specific transcription factors regulates the activity of Polymerase I and the transcription of rRNA in normal hematopoiesis, and how this regulation is co-opted in leukemia to drive abundant ribosome biogenesis. (upenn.edu)
Normal hematopoiesis requires an intricate balance in the bone marrow between the ability of stem cells to maintain themselves for decades of life while producing billions of mature blood cells every day. (upenn.edu)
It can also infiltrate other non-hematopoietic tissues and organs, inhibiting normal hematopoiesis in severe cases. (frontiersin.org)
Together these molecules constitute a ligand/receptor pair which functions to maintain normal hematopoiesis in the adult. (southernbiotech.com)

Furthermore, we revealed enhanced focal adhesion formation and osteogenic differentiation of MSCs cultured on positively charged protein fibers. (nature.com)
This study was therefore aimed at establishing the dose-response effect of EPO stimulation on rat BMSCs and investigating the effects of mechanical stretch combined with EPO on the proliferation and osteogenic differentiation of BMSCs. (hindawi.com)
The proliferation and osteogenic differentiation of rat BMSCs were examined and compared using EPO with different concentrations. (hindawi.com)
The osteogenic differentiation of BMSCs was detected by alkaline phosphatase (ALP) staining, and the mRNA expression levels of ALP, OCN, COL, and Runx2 were detected by qPCR. (hindawi.com)
Mechanical stretch combined with 20 IU/ml EPO promoted proliferation and osteogenic differentiation of BMSCs. (hindawi.com)
EPO was able to promote the proliferation and osteogenic differentiation of BMSCs, and these effects were enhanced when combined with mechanical stretch. (hindawi.com)
Notably, it promotes the osteogenic differentiation of bone mesenchymal stem cells (BMSCs) [ 15 - 17 ]. (hindawi.com)
This study was therefore aimed at addressing three key issues: First, is there a dose-dependent effect of EPO on the proliferation and osteogenic differentiation of BMSCs? (hindawi.com)
Transfection of MDS-MSCs with the miR-103-3p mimic improved osteogenic differentiation and decreased adipogenic differentiation in vitro, while inhibition of miR-103-3p showed the opposite results in NOR-MSCs. (techscience.com)
The miR-103-3p mimics may boost MDS-MSCs osteogenic differentiation while weakening lipid differentiation, thereby providing possible target for the treatment of MDS pathogenesis. (techscience.com)

The blood cell counts in TERT-depleted zebrafish embryos are markedly decreased and hematopoietic cell differentiation is impaired, whereas other somatic lineages remain morphologically unaffected. (harvard.edu)
These results suggest that TERT non-canonically functions in hematopoietic cell differentiation and survival in vertebrates, independently of its role in telomere homeostasis. (harvard.edu)

In summary, we demonstrate that the combination of single cell technologies with computational analysis tools enable the study of a variety of cellular mechanisms involved in complex biological systems such as early hematopoiesis and can be used to dissect perturbed differentiation trajectories associated with perturbations such as aging and malignant transformation. (cun.es)

Both C/EBPs command and control molecular programs of proliferation and differentiation into innate immune cells , including various types of myeloid cells. (mdc-berlin.de)
It maintains normal hematopoietic function by interacting with hematopoietic stem cells (HSCs) to control their proliferation and differentiation ( Morrison and Scadden, 2014 ). (techscience.com)
A variety of cytokines act synergistically with SCF to stimulate proliferation and differentiation of bone marrow progenitor cells. (southernbiotech.com)

They can also play a role in adult processes such as embryo implantation, hematopoiesis, and endothelial differentiation. (intechopen.com)
Since endothelial cell differentiation is one of the main steps to initiate vasculogenesis and angiogenesis, we analyzed the role of several Hox genes in the regulation of these two processes. (intechopen.com)
B lymphopoiesis is the result of several cell-commitment, lineage-choice, and differentiation processes. (eur.nl)
To date, the central role of specific transcription factors in positively regulating these distinct differentiation processes to acquire a B cell-specific genetic program is well established. (eur.nl)
Specifically, the processes by which the normal differentiation of cells is disrupted in cancer are of great interest. (uclahealth.org)
All-trans retinoic acid (RA) is the most active metabolite of vitamin A. Several studies have described a pivotal role for RA signalling in different biological processes such as cell growth and differentiation, embryonic development and organogenesis. (mdpi.com)
Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. (joplink.net)
We have developed and applied FACS-based high-resolution cell fractionation procedures to map the differentiation processes from HSCs. (lu.se)
SHP-2 is a critical component in several signalling pathways involved in the control of developmental processes, hematopoiesis, and metabolism. (lu.se)

Bone marrow-derived hematopoietic and mesenchymal stem cells as targets for Histoplasma capulatum infection: implications for hematopoiesis, differentiation, and proliferation. (urosario.edu.co)
To determine whether the interaction of hematopoietic stem cells (HSCs) and bone marrow-derived mesenchymal stem cells (BMMSCs) with Histoplasma capsulatum affects hematopoiesis, differentiation and proliferation of these precursor cells. (urosario.edu.co)
Hematopoiesis is an important model for stem cell differentiation with great medical significance. (europa.eu)
These genes play a central role during embryonic development by controlling body organization, organogenesis, and stem cell differentiation. (intechopen.com)
The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. (techscience.com)
MYB is well recognized to be a key regulator of definitive hematopoiesis that plays an important role in the maintenance and multilineage differentiation of hematopoietic stem cells (HSCs). (nih.gov)
To explore the role of MYB in human hematopoietic development we have inactivated the gene by bi-allelic TALEN-supported gene targeting in several lines of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), and subjected these cells to hematopoietic differentiation in well-defined cell culture conditions. (nih.gov)
To address this knowledge gap, we developed an in vivo hematopoietic stem cell (HSC)-based large-scale CRISPR knockout screening platform to enable the genetic interrogation of hematopoiesis and broad aspects of immune cell function in vivo. (biorxiv.org)
At the Italian NIH, he began to study stem cell differentiation and the homeostatic regulation of hematopoiesis. (nfcr.org)
Early hematopoiesis is a continuous process in which hematopoietic stem and progenitor cells (HSPCs) gradually differentiate toward specific lineages. (cun.es)
Here we review kinetic modeling efforts applied to stem cell developmental systems with focus on hematopoiesis. (lu.se)
The ontogeny of hematopoietic stem cells (HSCs) and T-cell differentiation are driven by a series of gene expression programs over time. (chop.edu)
Hierarchical differentiation competence in response to retinoic acid ensures stem cell maintenance during mouse spermatogenesis. (southernbiotech.com)

We study the development of blood cells and the emergence of leukemia by focusing on the molecular genetics of self-renewal and its connection to differentiation into functional cells of the innate immune system, like macrophages, granulocytes, and dendritic cells. (mdc-berlin.de)
A broader interest of our laboratory is to understand the molecular regulation of HSCs both from the perspectives of self-renewal and differentiation, and how these features become altered upon disease-causing or disease-associated perturbations. (lu.se)

New treatment strategies have pursued the idea of using mesenchymal stromal cells (MSCs) in combination with osteoinductive materials to guide differentiation of MSCs into bone cells ensuring complete bone regeneration. (nature.com)
This pure protein-based and chemically modifiable, fibrous ECM model allows the investigation of MSC behavior on biomimetic materials to unfold new vistas how to direct cells' differentiation for the development of new bone regenerating strategies. (nature.com)
Therefore, it is important to understand how MSC differentiation into bone cells is controlled and how this process can be guided or improved by externally added factors. (nature.com)
Interaction of MSCs with bone morphogenic proteins (BMPs) in an alginate scaffold mimicking the extracellular matrix (ECM) in vivo enhances their differentiation into bone cells 9 . (nature.com)
Furthermore, mechanical stretch may induce the differentiation of BMSCs into mature osteoblasts and enhance the deposition of the bone matrix [ 18 - 20 ]. (hindawi.com)

During embryonic development, hematopoiesis occurs through primitive and definitive waves, giving rise to distinct blood lineages. (lu.se)

Thus, conserved endocytic functions may control blood cell progenitor quiescence and differentiation. (nih.gov)
Here, we propose to uncover the hierarchy and regulatory mechanisms controlling hematopoiesis by combining comprehensive single-cell RNA-Seq analyses, modelling approaches, advanced functional assays, single-cell CRISPR screens, knockout models and epigenetic profiling. (europa.eu)
We will pursue the following aims: (i) Generate a refined model of hematopoiesis by comprehensive single-cell RNA-Seq profiling of hematopoietic progenitors, (ii) validate the predicted model by in vivo functional developmental assays and then (iii) test candidate transcription and chromatin factors uncovered by our model for their role in controlling progression towards various lineages using single-cell measurements combined with CRISPR screens. (europa.eu)
Histamine and its receptors represent a complex system of immunoregulation with distinct effects mediated by four GPCRs (G protein-coupled receptors HRs 1-4) and their differential expression, which changes according to the stage of cell differentiation and microenvironmental influences. (hindawi.com)
In this chapter, we summarized the evidence to support the function of Hox genes in adult tissues, specifically in endothelial cell differentiation, by studying their mechanism of action and how their target genes regulate vasculogenesis and angiogenesis. (intechopen.com)
Together with the associated research group of Dr. Alexander Mildner we perform ATACseq, ChIPseq and single cell analysis to disclose fundamental epigenetic mechanisms in hematopoiesis, homeostasis, autoimmune diseases, and in leukemogenesis. (mdc-berlin.de)
Hematopoietic transcription factors (= gene regulatory proteins) concertedly control cell proliferation, lineage commitment, and cell differentiation. (mdc-berlin.de)
The ability to perform lineage tracing at the single-cell level is critical to reconstructing dynamic transitions during cell differentiation. (biorxiv.org)
Trajectory inference revealed that altered gene expression dynamics accompanied cell differentiation, which could explain age-associated changes in hematopoiesis. (cun.es)
His laboratory is using the integrative approach to dissect the gene regulatory networks controlling embryonic hematopoiesis, T cell differentiation, and oncogenesis. (chop.edu)
and single-cell analysis of embryonic hematopoiesis and tumor microenvironment. (chop.edu)
For example, SCF plus IL-7 can stimulate the combined myeloid and B cell differentiation of uncommitted hematopoietic progenitor cells. (southernbiotech.com)
Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)
The activating PTPN11 variations play a broad role in cancer, because SHP-2 acts as a signal-enhancing signalling component in pathways that regulate cell growth, transformation, differentiation, and migration. (lu.se)
These data demonstrated that in older adults, COVID-19 mRNA vaccination elicited a lower frequency of Spike-specific CD4+ and CD8+ T cells producing cytokines involved in T cell differentiation and proliferation. (cdc.gov)
Abnormal proliferation, clonal expansion, aberrant differentiation, and diminished apoptosis (programmed cell death) lead to replacement of normal blood elements with malignant cells. (msdmanuals.com)
These malignancies are further characterized by the maturity and differentiation of the individual cell types and are divided into acute leukemias such as acute myeloid leukemia and acute lymphoblastic leukemia and chronic leukemias such as chronic myeloid leukemia and chronic lymphocytic leukemia. (medscape.com)
Different characteristics of the nuclei and cytoplasm of the cell allow differentiation by instrumentation and microscopy. (medscape.com)

Here we report that mutation in a conserved endocytic protein Asrij affects signal transmission and causes aberrant lymph gland hematopoiesis. (nih.gov)
Upon zTERT knockdown, zebrafish embryos show reduced telomerase activity and are viable, but develop pancytopenia resulting from aberrant hematopoiesis. (harvard.edu)

This has allowed for direct assessments of the molecular properties that associate with distinct developmental stages of hematopoiesis, and represents a powerful experimental paradigm when for instance applied to the study of experimental transgenic models. (lu.se)

The Paralkar Lab is interested in gaining a detailed mechanistic understanding of how chromatin proteins regulate the stemness-differentiation balance, and how mutations in them produce malignancy. (upenn.edu)
Mutations in these key transcription factors dysregulate hematopoiesis and cause diseases, such as leukemia and immune defects. (mdc-berlin.de)

Our findings reveal that during EHT, metabolism is a major regulator of primitive versus definitive hematopoietic differentiation. (lu.se)

With this biomimetic in vitro model we identified the fibrous structure as well as the charge of the material to be responsible for its effects on MSC differentiation. (nature.com)
Nevertheless, MSCs can lose their differentiation potential after expansion in vitro and transplantation into the site of injury 7 . (nature.com)
Our findings may help explain human genetic associations and identify a novel genetic strategy to enhance in vitro hematopoiesis. (biomedcentral.com)
Thus, our framework stratified relevant functional loci and helped identify TPM1 manipulation as a novel strategy to enhance in vitro hematopoiesis. (biomedcentral.com)

Histopathologic examination showed diffused liver fibrosis with cystic dilatation of the bile ducts and focal extramedullary hematopoiesis. (cdc.gov)

The RUNX1 protein turns on (activates) genes that help control the development of blood cells (hematopoiesis). (medlineplus.gov)

In the adult, HSC quiescence, maintenance, and differentiation are closely linked to changes in metabolism. (lu.se)
In contrast, OXPHOS-mediated differentiation of HE toward definitive hematopoiesis is dependent on cholesterol metabolism. (lu.se)

This change in gene activity blocks the maturation (differentiation) of blood cells and leads to the production of abnormal, immature white blood cells called myeloid blasts. (medlineplus.gov)

It regulates Th2 differentiation and lineage-specific hematopoiesis. (thermofisher.com)

For a previously unpublished BMP6 study, GAGE predicted novel regulatory mechanisms for BMP6 induced osteoblast differentiation, including the canonical BMP-TGF beta signaling, JAK-STAT signaling, Wnt signaling, and estrogen signaling pathways-all of which are supported by the experimental literature. (biomedcentral.com)
In this review, we focus on the impact of ncRNA post-transcriptional regulatory mechanisms, especially those of microRNAs and lncRNAs, in RA signalling pathways during differentiation and disease. (mdpi.com)
This highlights the flexibility and adaptability of the control mechanisms that govern hematopoiesis. (lu.se)

In the vertebrate developmental context, MYB is widely regarded dispensable for primitive hematopoiesis but critically required for the development of definitive hematopoiesis. (nih.gov)
Although both primitive and definitive hematopoiesis is disrupted by zTERT knockdown, the telomere lengths are not significantly altered throughout early development. (harvard.edu)

We apply targeted mouse genetics in combination with experimental hematopoiesis, genetic engineering of hematopoietic cells, human iPSC hematopoietic differentiation, proteomics and biochemistry. (mdc-berlin.de)
In this study, we used an enrichment of scRNA-seq mitochondrial reads and a robust computational method to identify clonally relevant mitochondrial variants as endogenous genetic barcodes for clonal tracking of early embryonic hematopoiesis from hPSC. (biorxiv.org)
Every differentiation step is characterized by the activation of a new, lineage-specific, genetic program and the extinction of the previous one. (eur.nl)

Venus gene reporter was inserted into the knock-in allele to monitor MYB expression during the course of the hESC/iPSC differentiation. (nih.gov)

BMMSCs) with Histoplasma capsulatum affects hematopoiesis, differentiation and proliferation of these precursor cells. (urosario.edu.co)
Dysregulation of these master switches may affect differentiation, which can lead to "confused" cells as the potential progenitors of leukemia. (mdc-berlin.de)
Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. (joplink.net)
A basic helix-loop-helix transcription factor that plays a critical role in HEMATOPOIESIS and as a positive regulator in the differentiation of ERYTHROID CELLS. (bvsalud.org)

We demonstrate that glycolysis-mediated differentiation of HE toward primitive erythroid hematopoiesis is dependent on the epigenetic regulator LSD1. (lu.se)

In this context, he is studying the role of non-coding RNA and post-transcriptional gene expression regulation in hematopoiesis and malignancy. (uclahealth.org)

Due to the hierarchical structure of hematopoiesis, we have suggested that many of these changes can be associated with an altered HSC function with age. (lu.se)

This is therefore the first demonstration of a non-canonical catalytic activity of TERT, which is different from 'authentic'' telomerase activity, is required for during vertebrate hematopoiesis. (harvard.edu)

Blocking TNF reversed this defect in emergency hematopoiesis but, surprisingly, Tnfr1 deficiency did not prevent inflammation in Ripk1(-/-) neonates. (nih.gov)
On the other hand, zTERT deficiency induced a defect in hematopoiesis through a potent and specific effect on the gene expression of key regulators in the absence of telomere dysfunction. (harvard.edu)

Here, we show that the emergence of blood is regulated by multiple metabolic pathways that induce or modulate the differentiation toward specific hematopoietic lineages during human EHT. (lu.se)

Loss of Tada2b or Taf5l inhibited hematopoiesis in vivo and was associated with upregulation of interferon response gene expression. (biorxiv.org)

Surprisingly, only the reverse transcriptase motifs of zTERT are crucial, but the telomerase RNA-binding domain of zTERT is not required, for rescuing complete hematopoiesis. (harvard.edu)

Anatomy61

Organisms13

Diseases13

Chemicals and Drugs83

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes58

Disciplines and Occupations1

Information Science3

Erythroid Differentiation2

Blood cell differentiation5

Human hematopoiesis3

Clonal hematopoiesis3

Normal hematopoiesis4

Osteogenic differentiation10

Hematopoietic cell differentiation2

Perturbed differentiation trajectories1

Proliferation and differentiation3

Processes9

Stem13

Self-Renewal2

Bone5

Embryonic development1

Cell18

Aberrant2

Molecular1

Mutations2

Regulator of primitive1

Vitro4

Extramedullary1

Genes1

Metabolism2

Myeloid1

Regulates1

Mechanisms3

Definitive2

Genetic3

IPSC1

Cells4

Primitive1

Gene Expression1

Hierarchical1

Vertebrate1

Defect2

Induce1

Vivo1

Surprisingly1