• Furthermore, the study examined the effects of cooperating oncoproteins such as Ras or Bcr/Abl, which allowed for both factor-independent proliferation and differentiation, or Bcl-2, which permitted factor-independent survival but not proliferation. (nih.gov)
  • It maintains normal hematopoietic function by interacting with hematopoietic stem cells (HSCs) to control their proliferation and differentiation ( Morrison and Scadden, 2014 ). (techscience.com)
  • All together, the results of the present study demonstrated that hGM-CSF can stimulate the proliferation and differentiation of primitive and definitive erythroid cells independently of EPOR signal if they express hGMR, and the activity is comparable to that of EPO in definitive, but not primitive, erythropoiesis. (elsevierpure.com)
  • It enhances granulocyte proliferation and differentiation [7]. (peertechzpublications.com)
  • It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. (uchicago.edu)
  • The G-CSF receptor is expressed on a variety of hematopoietic cells, including myeloid-committed progenitor cells, neutrophils, granulocytes, and monocytes. (stemcell.com)
  • Secondary endpoints were systemic cytokine concentrations, leukocyte counts, and the phagocytic activity of granulocytes and monocytes. (asahq.org)
  • Fluorescent histograms were derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes (left panel), monocytes (center panel) and granulocytes (right panel). (bdbiosciences.com)
  • Therefore, the present study aimed to evaluate the influence of HMB on the chemotactic activity (MIGRATEST® kit), phagocytic activity (PHAGOTEST® kit) and oxidative burst (BURSTTEST® kit) of monocytes and granulocytes in the peripheral blood of calves by flow cytometry. (researchsquare.com)
  • HMB also had a positive effect on the oxidative metabolism of monocytes and granulocytes stimulated with PMA (4-phorbol-12-β-myristate-13-acetate) and Escherichia coli bacteria, expressed as MFI values and the percentage of oxidative metabolism. (researchsquare.com)
  • The differentiation of Socs3-deficient progenitor cells is skewed toward macrophage production in response to G-CSF or IL-6, whereas wild-type progenitor cells produce mainly neutrophils. (nih.gov)
  • It is a key cytokine involved in the production of neutrophils and the stimulation of granulocyte colony formation from hematopoietic progenitor cells (Metcalf & Nicola). (stemcell.com)
  • Ectopic expression of AML1-MTG8 in L-G murine myeloid progenitor cells inhibits differentiation to mature neutrophils and induces cell proliferation in response to granulocyte colony-stimulating factor (G-CSF). (embl-heidelberg.de)
  • It stimulates the survival, proliferation, differentiation and function of neutrophil granulocyte progenitor cells and mature neutrophils. (allindianpatents.com)
  • describes a novel colony stimulating factor (CSF) that has the ability to promote the differentiation and proliferation of human bone marrow cells to neutrophils, and a method to produce the same from a novel cell line which has been established from tumor cells in patients with oral cancer, The most published studies have used filgrastim as it was the first form of G-CSF to be approved. (allindianpatents.com)
  • We isolated total CD34 + cells, CD34 + ,CD33 + cells, and CD34 + ,CD33 - cells individually from normal human bone marrow cells by fluorescence-activated cell sorter (FACS), and examined the effects of granulocyte colony-stimulating factor (G-CSF), interleukin-3 (IL-3), and IL-5 on in vitro colony formation of these cells. (elsevierpure.com)
  • Transfection of MDS-MSCs with the miR-103-3p mimic improved osteogenic differentiation and decreased adipogenic differentiation in vitro, while inhibition of miR-103-3p showed the opposite results in NOR-MSCs. (techscience.com)
  • To evaluate the leukemogenic role of RUNX1-MT in ASXL1 -mutated cells, we co-expressed RUNX1 -MT (R135T) and ASXL1 -MT (R693X) in different cell lines and performed immunoblot, co-immunoprecipitation, gene expression microarray, quantitative RT-PCR, cell proliferation, differentiation, and clonogenic assays for in vitro functional analyses. (biomedcentral.com)
  • We found that in vitro, this interaction alters the ability of RUNX1 to bind to DNA and to regulate a reporter gene, whereas in vivo, the expression of the isolated 8th zinc finger motif of EVI1 is sufficient to block the granulocyte colony-stimulating factor-induced differentiation of 32Dcl3 cells, leading to cell death. (lincoln.ac.uk)
  • This result is particularly significant since 32D cells do not spontaneously express the CSF-1 receptor, whereas they undergo granulocytic differentiation upon G-CSF stimulation. (mcmaster.ca)
  • Although erythropoietin (EPO) and its receptor (EPOR) are crucial for the proliferation, survival, and terminal differentiation of erythroid progenitors, it remains to be elucidated whether EPOR-unique signaling is required for erythropoiesis. (elsevierpure.com)
  • We demonstrate that the inhibitory receptor LILRB1 (ILT2, LIR1, CD85j) is selectively up-regulated during DC differentiation from monocyte precursors in culture. (ashpublications.org)
  • In order to determine which elements of cellular responses to cytokines require Socs3, we have examined the differentiative and proliferative capacity of hematopoietic progenitor cells stimulated by G-CSF and IL-6. (nih.gov)
  • Strikingly, synergistic responses to costimulation with stem cell factor and IL-6 (but not G-CSF) are lost at higher concentrations in Socs3-deficient progenitor cells. (nih.gov)
  • CD34 + ,CD33 + cells formed granulocyte colonies in the presence of G-CSF. (elsevierpure.com)
  • Both CD34 + ,CD33 + cells and CD34 + ,CD33 - cells formed granulocyte/macrophage colonies in the presence of IL-3. (elsevierpure.com)
  • Eosinophil (Eo) colonies were only formed by CD34 + ,CD33 - cells in response to IL-3, but scarcely formed by CD34 + cells in the presence of IL-5. (elsevierpure.com)
  • CD34 - ,CD33 + cells derived from CD34 + ,CD33 + cells by preincubation with G-CSF or IL-3 formed Eo colonies in the presence of IL-5 but not IL-3. (elsevierpure.com)
  • CD34 - ,CD33 + cells derived from CD34 + ,CD33 - cells by preincubation with IL-3 also formed Eo colonies by support of IL-5 as well as IL-3. (elsevierpure.com)
  • It was concluded that G-CSF supported the neutrophil differentiation of committed colony-forming cells, IL-3 supported that of both committed and multipotent colony-forming cells. (elsevierpure.com)
  • The differentiation of the cells could be arrested by heterologous oncoproteins including AML1/ETO, PML/RARalpha, PLZF/RARalpha, Nup98/HoxA9, and other Hox proteins. (nih.gov)
  • The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. (techscience.com)
  • Results An analysis of granulocyte and monocyte chemotactic activity and phagocytic activity revealed significantly higher levels of phagocytic activity in calves administered HMB than in the control group, expressed in terms of the percentage of phagocytising cells and mean fluorescence intensity (MFI). (researchsquare.com)
  • Dendritic cells (DCs) orchestrate inflammatory immune response in the airways, but little is known about how organic dust affects differentiation and maturation of monocyte-derived immature and mature DCs (iDCs, mDCs). (cdc.gov)
  • Granulocyte Colony Stimulating Factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. (rndsystems.com)
  • Blood stem cells with low levels of Sca-1 differentiated into red blood cell progenitors seven times more often than cells high in Sca-1 when exposed to erythropoietin, a growth factor that promotes red blood cell production. (sciencedaily.com)
  • Huang and Chang then looked at the proteins GATA1 and PU.1, transcription factors that normally favor differentiation into red and white blood cells, respectively. (sciencedaily.com)
  • Although, a list of contributing factors such as sickle cell anaemia, use of steroids, femoral neck fractures are involved in the loss of osteogenic cells via obstructing blood supply especially in the greater trochanteric region[ 1 , 2 ]. (ijpsonline.com)
  • MSCs have multilineage differentiation potential, allowing them to differentiate, for example, into chondrocytes and osteoblasts, the key cells from the two tissues that constitute the osteochondral unit. (hindawi.com)
  • Here we show that 32D cells transformed with two different oncogenes, v-src or c-fms [S301,F969], both belonging to the CSF-1 transduction pathway, respond to exogenous wt-p53 expression with the same final outcome-monocytic differentiation. (mcmaster.ca)
  • In methylcellulose clonal culture of fetal liver (FL) cells of generated hGMR-expressing EPOR -/- embryos at embryonic day (E) 12.5 of gestation, hGM-CSF stimulated erythroid colony formation under serum-containing and serum-free conditions. (elsevierpure.com)
  • Analysis of globin expression in individual erythrocyte-containing colonies formed from E12.5 FL cells showed that hGM-CSF supports primitive and definitive erythropoiesis even in EPOR -/- embryos. (elsevierpure.com)
  • and induced increase of GATA-1 expression and decrease of erythroid Kruppel-like factor and cMyb expression in the FL cells. (elsevierpure.com)
  • Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells. (cusabio.com)
  • Until recently, differentiation was thought to be the primary function of regenerative cells. (vetstem.com)
  • The expression of RUNX1 mutant in ASXL1 -mutated myeloid cells augmented proliferation, blocked differentiation, and increased self-renewal activity. (biomedcentral.com)
  • Tofacitinib also may inhibit the differentiation of T-helper lymphocytes (type 1 and type 2) and inhibit type 17 T-helper cells. (aad.org)
  • In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)
  • IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. (thermofisher.com)
  • Binds to cell surface receptors on hematopoietic cells and stimulates proliferation, differentiation, and some end-cell functional activation. (hemonc.org)
  • Continuous ligation of LILRB1 modulated cellular differentiation, conferred a unique phenotype upon the resultant cells, induced a profound resistance to CD95-mediated cell death, and inhibited secretion of cytokines IL-10, IL-12p70, and TGF-β. (ashpublications.org)
  • Herein, we demonstrate that myostatin propeptide expands primitive CML and normal BM cells, as shown by increased colony-forming capacity. (haematologica.org)
  • function of proliferative responses provoked in the induction cell products favor immediate type hypersensitivity reactions, phase of contact allergy, the murine local lymph node assay serving as growth and differentiation factors for mast cells and (LLNA), has been accepted as a stand-alone method for skin eosinophils, the cellular effectors of the clinical manifestations of sensitization testing (NIH, 1999). (cdc.gov)
  • Trisomy 8+ cells showed a significant positive correlation with apoptotic CD34+ cells and capacity for colony formation. (who.int)
  • The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)
  • Reinforcement effect of histamine on the differentiation of murine myeloblasts and promyelocytes: externalization of granulocyte colony-stimulating factor receptors induced by histamine. (aspetjournals.org)
  • 1 Activation of DCs through toll-like receptors (TLRs) for "infectious nonself" or other "danger" signals normally initiates a process of cellular differentiation resulting in "mature" DCs capable of stimulating T-cell and natural killer (NK)-cell responses. (ashpublications.org)
  • To study the relationship between hematopoietic factors and their responsive hematopoietic progenitors in the differentiation process, both purified factors and enriched progenitors are required. (elsevierpure.com)
  • Granulocyte colony stimulating factor (G-CSF) is a 20 kDa glycoprotein cytokine that stimulates growth and development of granulocyte progenitors in the bone marrow (Nagata et al. (allindianpatents.com)
  • MDSCs recovered from BRQ-treated mice showed signs of greater maturation, supporting the hypothesis that BRQ forces the differentiation of myeloid progenitors. (health.mil)
  • Granulocyte colony-stimulating factor (G-CSF) and interleukin-6 (IL-6) play key roles in regulating emergency granulopoiesis and inflammation, and are both negatively regulated by the inducible intracellular protein suppressor of cytokine signaling-3 (Socs3). (nih.gov)
  • Granulocyte colony-stimulating factor (G-CSF) is a hematopoietic cytokine appears in materno-fetal interface during embryo implantation and early pregnancy suggesting it may have a role in decidua and placental development [6]. (peertechzpublications.com)
  • Organic dust exposure alters monocyte-derived dendritic cell differentiation and maturation. (cdc.gov)
  • Dust exposure alters monocyte differentiation to iDCs and prevents maturation of iDC to mDCs. (cdc.gov)
  • The first 48 h of monocyte differentiation appears to be the susceptible period to exposure. (cdc.gov)
  • Environmental exposures present during early monocyte differentiation may impact the critical balance of DCs in the lung. (cdc.gov)
  • Planned Granulocyte Colony-Stimulating Factor Adversely Impacts Survival after Allogeneic Hematopoietic Cell Transplantation Performed with Thymoglobulin for Myeloid Malignancy. (uchicago.edu)
  • Growth factors and cytokines involved in their migration, development and survival could be absent in this tissue and need further investigations. (bvsalud.org)
  • A major limitation in approaching this problem has been the lack of suitable cell lines that exhibit normal, terminal, and synchronous differentiation in the absence of endogenous oncoproteins and in response to physiologic cytokines, and whose differentiation can be arrested by ectopically expressed human oncoproteins. (nih.gov)
  • This covers the production of several cytokines and chemokines including interleukin 1 (IL-1), interleukin 6 (IL-6), tumor necrosis factor- alpha (TNF-α) and chemokine (C-C motif) ligand 2 (CCL2) [ 4 ]. (biomedcentral.com)
  • Thrombopoiesis takes place primarily in the bone marrow through a series of cell differentiation and is influenced by several cytokines. (lecturio.com)
  • Factor that negatively affected growth in the historic period was effect of Covid-19, low number of drug approvals, and lack of awareness on biosimilars among primary care physicians and specialists. (thebusinessresearchcompany.com)
  • Factors that could hinder the growth of the rituximab biosimilar market in the future include stringent regulations related to biosimilar approvals, and prolonged production and approval time. (thebusinessresearchcompany.com)
  • The additional application of a bone marrow aspirate (BMA) to the procedure of marrow stimulation is thought to enhance cartilage repair as it may provide both an additional cell population capable of chondrogenesis and a source of growth factors stimulating cartilage repair. (hindawi.com)
  • In the orthopaedic field, additional application of a bone marrow aspirate (BMA) to the procedure of marrow stimulation has been recently studied, since the bone marrow itself is both a source of MSCs, providing a cell population capable of chondrogenesis and of various growth factors stimulating cartilage repair [ 7 - 10 ]. (hindawi.com)
  • Expression of exogenous wt-p53 in different tumor cell lines can induce growth arrest, apoptosis, or differentiation. (mcmaster.ca)
  • The utilization of Platelets and other growth factors found in your blood, trigger new collagen production. (jointhealthusa.com)
  • IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. (thermofisher.com)
  • PGRN has been introduced among the secreted growth factors as one related to wound healing, inflammation, and human embryonic development, as well as a wide variety of autoimmune diseases. (biomedcentral.com)
  • Retroviral transduction of a dominant negative CREB mutant reduced C/EBPβ mRNA levels and significantly impaired the proliferation/differentiation of granulocyte precursors, while a constitutively active form of CREB facilitated C/EBPβ transcription. (harvard.edu)
  • These myeloid cell lines provide tools for examining the biochemical and genetic pathways that accompany normal differentiation as well as a system in which to dissect how other leukemic oncoproteins interfere with these pathways. (nih.gov)
  • Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. (uchicago.edu)
  • In contrast to the definitive role of the transcription factor, CCAAT/Enhancer binding protein α (C/EBPα), in steady-state granulopoiesis, previous findings have suggested that granulopoiesis during emergency situations, such as infection, is dependent on C/EBPβ. (harvard.edu)
  • The transcription initiation TFIID complex is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). (embl-heidelberg.de)
  • The AML1-CBFbeta transcription factor complex is essential for the definitive hematopoiesis of all lineages and is the most frequent target of chromosomal rearrangements in human leukemia. (embl-heidelberg.de)
  • Previously, we observed that transcription factor RUNX1 mutations ( RUNX1 -MT) coexisted with ASXL1 -MT in CMML and at myeloid blast phase of chronic myeloid leukemia. (biomedcentral.com)
  • Control iDCs exposed to ODE during the final 2 days of iDC maturation (days 7 and 8) did not differ from control (no ODE) iDCs in surface marker expression and phagocytic ability, but exhibited enhanced lymphocyte-stimulating capacity. (cdc.gov)
  • Clones were identified that proliferated as immortalized blasts in the presence of estrogen, and that exhibited granulocytic, monocytic, or bipotential (granulocytic and monocytic) differentiation on estrogen withdrawal. (nih.gov)
  • The authors studied the effects of mild hypothermia on the outcome in a rat model of intraabdominal sepsis and tested whether granulocyte colony-stimulating factor (G-CSF) augments the host response and improves outcome during mild hypothermia. (asahq.org)
  • Differentiation was normal and terminal as evidenced by morphology, cell surface markers, gene expression, and functional assays. (nih.gov)
  • Additionally, IFN-γ signaling inhibition by the blockade of JAK1 decreases the production of tumor necrosis factor and IL-6. (aad.org)
  • Hematopoiesis depends on a tightly controlled balance of self-renewal, proliferation, cell death and differentiation. (nature.com)
  • Conclusion HMB stimulates non-specific cell-mediated immunity, which is a very important consideration in newborn calves that are exposed to adverse environmental factors in the first weeks of their life. (researchsquare.com)
  • Granulocyte Colony-Stimulating Factor Use after Autologous Peripheral Blood Stem Cell Transplantation: Comparison of Two Practices. (uchicago.edu)
  • In myocarditis, T cell differentiation can crucially contribute to aggravating or alleviating such conditions. (biomedcentral.com)
  • The molecular pathways of normal myeloid differentiation, as well as the mechanisms by which oncogenes disrupt this process, remain poorly understood. (nih.gov)
  • Thus, the expression of miR-103-3p decreases in MDS BM plasma and MDS-MSCs, significantly impacting MDS-MSCs differentiation. (techscience.com)
  • The miR-103-3p mimics may boost MDS-MSCs osteogenic differentiation while weakening lipid differentiation, thereby providing possible target for the treatment of MDS pathogenesis. (techscience.com)
  • Granulocyte Colony-Stimulating Factor" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (uchicago.edu)
  • On the other hand, in E8.0 yolk sac erythropoiesis, both substances had a similar effect on erythroid colony formation, but hGM-CSF induced an increase of β-major globin expression, while EPO did not. (elsevierpure.com)
  • It looks that the clinical pregnancy rates were significantly higher by intracavitary infusion of granulocyte colony stimulating factor. (peertechzpublications.com)
  • 8+ avaient une corrélation positive importante avec les cellules CD34+ apoptotiques et la capacité à stimuler la formation de colonies. (who.int)
  • Evidence suggest that a patient's genetic predisposition together with environmental factors, such as exposure to pathogens that exhibit molecular mimicry, disturb immune tolerance ( 4 ). (frontiersin.org)