• Chronic myeloid leukemia (CML) results from BCR-ABL oncogene, which blocks CML cells differentiation and protects these cells from apoptosis. (jcancer.org)
  • Here, we determined the effect of Jiyuan oridonin A (JOA), an ent -kaurene diterpenoid compound, on the differentiation blockade in imatinib-sensitive, particularly, imatinib-resistant CML cells with BCR-ABL-T315I mutation by cell proliferation assay, apoptosis analysis, cell differentiation analysis, cell cycle analysis and colony formation assay. (jcancer.org)
  • Its roles include regulation of proliferation, promotion of immune system and activation of apoptosis in B cells. (intechopen.com)
  • In these cells, IL-21 does not promote proliferation but enhances apoptosis and chemotaxis. (intechopen.com)
  • Excitingly, 416 effectively overcame ATRA resistance in vitro and in vivo by inducing apoptosis and differentiation. (bvsalud.org)
  • CTCL cell apoptosis, DC differentiation and apoptotic malignant T cell ingestion were measured by immunostaining. (biomedcentral.com)
  • After CD8 T cells were stimulated by DC loaded with malignant cells, they mediated increased apoptosis of residual CTCL cells and TNF-α secretion indicating development of enhanced cytolytic function. (biomedcentral.com)
  • The antitumor effect of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in gliomas has thus far only been thoroughly established in tumor cell lines. (aacrjournals.org)
  • We isolated primary tumor cells from 13 astrocytoma and oligoastrocytoma patients of all four WHO grades of malignancy and compared the levels of TRAIL-induced apoptosis induction, long-term tumor cell survival, caspase, and caspase target cleavage. (aacrjournals.org)
  • Taken together, the current results demonstrate that MAPK1 promotes myeloid cell proliferation and differentiation simultaneously by cell cycle progression while suppresing apoptosis. (prinsesmaximacentrum.nl)
  • Murine PD-1 mRNA expression has been shown to be correlated with activation-induced apoptosis in a mouse T-cell hybridoma cell line and murine thymocytes [ 13 ]. (biomedcentral.com)
  • The MDS cell of origin is a hematopoetic stem cell that proliferates and escapes apoptosis. (standardofcare.com)
  • AA005 induces a unique type of cell death in colorectal adenocarcinoma cells, characterized by lack of caspase-3 activation or apoptotic body formation, sensitivity to poly (ADP-ribose) polymerase inhibitor Olaparib (AZD2281) but not pan-caspase inhibitor Z-VAD.fmk, and dependence on apoptosis-inducing factor (AIF). (biomedcentral.com)
  • The TP53 gene is also capable of stimulating apoptosis of cells containing damaged DNA. (medscape.com)
  • TP53 activates the expression of genes involved in apoptosis, cell cycle regulation (p21), and MDM2. (medscape.com)
  • Several years later, Kerr and co-workers 2 defined the morphological changes in cells undergoing this process, also known as apoptosis. (nature.com)
  • We discuss here the contribution of glycan-lectin interactions to the initiation, execution and resolution of apoptosis and their emerging roles in other cell death programs including autophagy. (nature.com)
  • Apoptosis was determined by cell death assay and western blotting. (biomedcentral.com)
  • Triphala-mediated reduced cell survival correlated with induction of apoptosis, which was associated with reactive oxygen species (ROS) generation. (biomedcentral.com)
  • Triphala-induced apoptosis was linked with phosphorylation of p53 at Ser-15 and ERK at Thr-202/Tyr-204 in Capan-2 cells. (biomedcentral.com)
  • Pretreatment of cells with pifithrin-α or U0126, specific inhibitors of p53 or MEK-1/2, significantly attenuated Triphala-induced apoptosis. (biomedcentral.com)
  • Similarly, Triphala induced apoptosis in another pancreatic cancer cell line BxPC-3 by activating ERK. (biomedcentral.com)
  • On the other hand, Triphala failed to induce apoptosis or activate ERK or p53 in normal human pancreatic ductal epithelial (HPDE-6) cells. (biomedcentral.com)
  • Reduced tumor-growth in Triphala fed mice was due to increased apoptosis in the tumors cells, which was associated with increased activation of p53 and ERK. (biomedcentral.com)
  • Abnormal proliferation, clonal expansion, aberrant differentiation, and diminished apoptosis (programmed cell death) lead to replacement of normal blood elements with malignant cells. (msdmanuals.com)
  • In this chapter, we will focus on IL-21's biological effects and signaling pathways as well as discuss the potential implications and applications of IL-21 in leukemia cells. (intechopen.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Using an AML mouse model driven by expression of AML1/ETO9a (A/E9a), we demonstrated that treatment of mice bearing t(8;21) AML with the HDACi panobinostat caused a robust antileukemic response that did not require functional p53 nor activation of conventional apoptotic pathways. (ashpublications.org)
  • Yet, NOX2 can also be recruited to the plasma membrane of phagocytes leading to the generation of extracellular H 2 O 2 ( Aviello and Knaus, 2018 ), and NOX2-derived ROS participate in major signaling pathways, both within the individual phagocyte and surrounding cells. (frontiersin.org)
  • Therefore, the aim of the present study was to investigate the gene expression patterns following siRNA‑mediated suppression of RUNX1‑RUNX1T1 and MAPK1 in Kasumi‑1 and SKNO‑1 cells and to determine the differentially expressed genes in enriched biological pathways. (prinsesmaximacentrum.nl)
  • To demonstrate the power of this technology, we will generate a molecular disease fingerprint allowing differentiation between three clinically indistinguishable yet biochemically distinct disease pathways underlying the deadly brain cancer glioblastoma multiforme. (nih.gov)
  • The approval of the anti apoptotic BCL2 antagonist venetoclax has fi nally validated the potential of targeting apoptotic pathways in patients with cancer. (ibecbarcelona.eu)
  • Since it is known that inhibition of cell death mechanisms is a common event in tumour development, this poses problems for many forms of chemotherapy that utilise cell death pathways, leading to drug resistance. (gla.ac.uk)
  • In this review, we highlight that many of the key regulatory pathways of the cell are recruited by motifs and that the ease of motif acquisition has resulted in large networks of co-regulated biomolecules. (biomedcentral.com)
  • 10 Galectins can function inside the cells by modulating signaling pathways, 11 although they also act extracellularly by establishing multivalent interactions with cell surface glycans and delivering signals that lead to disruption of cellular homeostasis. (nature.com)
  • Overall we show that NOX2, through the regulation of NOX4 expression, controls metabolism and mitochondrial function in CML cells. (biomed.news)
  • In addition, hyperpolarized tumor cell membranes and mitochondrial membrane potentially allow selective accumulation of mitochondria-targeted drugs. (biomed.news)
  • Here, we demonstrate that mitochondria and mitochondrial-derived vesicles (MDVs) are secreted from mature osteoblasts to promote differentiation of osteoprogenitors. (biomed.news)
  • In addition, distinct NOX homologs expressed by non-phagocytic cells and mitochondrial ROS are interlinked with phagocytic NOX functions and thus affect the overall redox state of the tissue and the cellular activity in a complex fashion. (frontiersin.org)
  • RUNX1‑RUNX1T1 suppression exerted an anti‑apoptotic effect through the overexpression of BCL2, BIRC3 and CFLAR genes, while MAPK1 suppression induced apopotosis in t(8;21) cells by the apoptotic mitochondrial changes stimulated by the activity of upregulated TP53 and TNFSF10, and downregulated JUN gene. (prinsesmaximacentrum.nl)
  • Annonaceous acetogenin mimic AA005 reportedly inhibits mammalian mitochondrial NADH-ubiquinone reductase (Complex I) and induces gastric cancer cell death. (biomedcentral.com)
  • Additionally, Trx2 right related with UCP3 as a result of a cell cycle activation mitochondrial targeting signaling sequence, was processed inside the intermembrane space, and thereby allowing redox reactions. (aurorapathway.com)
  • All trans-retinoic acid (ATRA) induces acute promyelocytic leukemic NB4 cells along granulocytic differentiation. (bvsalud.org)
  • Ready access to both the DC and apoptotic cells provided by this system will allow extension to other malignancies through the addition of a variety of apoptotic tumor cells and maturation stimuli. (biomedcentral.com)
  • In the ECP treatment, both apoptotic CTCL cells and transitioning DC are re-infused into the patient immediately and association of the DC and apoptotic tumor cells occurs inefficiently in vivo . (biomedcentral.com)
  • The transimmunization procedure was devised as a more effective modification of ECP and named to designate the transfer of tumor antigens to competent antigen presenting cells (APC) that could display the full complement of tumor antigens in the context of co-stimulatory and adhesion molecules. (biomedcentral.com)
  • However, as tumors progress, cancerous cells develop immunosuppressive mechanisms that circumvent NK cell-mediated killing, allowing for tumor escape and proliferation. (frontiersin.org)
  • Therapeutic intervention aims to reverse tumor-induced NK cell suppression and sustain NK cells' tumorlytic capacities. (frontiersin.org)
  • Here, we review tumor-NK cell interactions, discuss the mechanisms by which NK cells generate an antitumor immune response, and discuss NK cell-based therapeutic strategies targeting activating, inhibitory, and co-stimulatory receptors. (frontiersin.org)
  • instead of targeting tumor cells, the goal of immunotherapy is to augment and expand the immune system's intrinsic antitumor response. (frontiersin.org)
  • First described in 1975, NK cells were initially identified as a distinct sub-population of lymphocytes by their capacity to spontaneously lyse tumor cells ( 1 ). (frontiersin.org)
  • NK cells are now accepted to play an important role in both the adaptive and innate immune responses that govern infection, autoimmunity, and tumor immunosurveillance ( 2 , 3 ). (frontiersin.org)
  • NK cell infiltration into tumor tissue is associated with better disease prognosis in colorectal cancer, clear cell renal cell carcinoma, and lung carcinomas ( 9 - 11 ). (frontiersin.org)
  • In contrast to cell lines, isolated primary tumor cells from all investigated glioma patients were highly TRAIL resistant. (aacrjournals.org)
  • This enhances the direct killing of tumor cells by drug molecules while minimizing the potential toxicity to normal cells. (biomed.news)
  • A novel p53-derived apoptotic peptide de-represses p73 to cause tumor regression in vivo. (gla.ac.uk)
  • Moreover, increasing data have shown that peripheral T-cell tolerance is an essential property of the specific immune response to tumor cells. (biomedcentral.com)
  • These changes can be inherited and are, therefore, found in every cell, but more often, they are somatically acquired and restricted to tumor cells. (medscape.com)
  • Inactivation of tumor suppressor genes, whose products normally provide negative control of cell proliferation, contributes to malignant transformation in various cell types. (medscape.com)
  • Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)
  • Another important class of tumor suppressor genes involved in cell cycle control and in the generation of human cancers is the cyclin-dependent kinase (CDK) inhibitors. (medscape.com)
  • Exosomes, which are 30- to 120-nm vesicles, are released by most types of cells, including tumor cells. (bbrc.in)
  • Recently, it has been reported by many workers that the potential functions of exosomes contributed to various aspects of hematological tumorigenesis, particularly with a focus on the exosome-mediated tumor progression, metastasis, drug resistance and immune escape by altering the function of receiver cells via diverse exosomal cargoes including proteins, DNA, messenger RNAs (mRNAs), and microRNAs) (Whiteside and Boyiadzis, (2017). (bbrc.in)
  • X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. (lonza.com)
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL). (lonza.com)
  • By exogenously modulating its activity, we demonstrate that PP1α is required to maintain CHK1 in a dephosphorylated state and, more importantly, that it is responsible for the accelerated replication fork progression in Spi1/PU.1-overexpressing cells. (oncotarget.com)
  • Although genomic alterations have been extensively characterized in AML [ 1 ], little is known about the determining events that favor the accumulation of mutations and the progression from the pre-leukemic to leukemic stage. (oncotarget.com)
  • We show that negatively regulates T\ALL cell growth and cell cycle progression but has no effect on apoptotic cell death. (thetechnoant.info)
  • We postulate that is epigenetically deregulated in T\ALL and serves as an important regulator of T\ALL cell proliferation and leukemic progression. (thetechnoant.info)
  • Given the roles of in normal T cell development, we hypothesized that SOCS5 is a critical mediator of JAK\STAT signaling and T\ALL progression. (thetechnoant.info)
  • Moreover, T cell function becomes suppressed with disease progression. (biomedcentral.com)
  • A heterogenous group of myeloid disorders characterized by somatically mutated hematopoietic stem cells,the presence of variable peripheral cytopenias, and a broad risk of progression to acute myeloid leukemia (AML). (standardofcare.com)
  • The mutation occurs in hematopoetic stem cells capable of self renewal and additional mutations are associated with clonal progression may occur in progenitor cells conferring a self renewal capability. (standardofcare.com)
  • The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)
  • Chronic myeloid leukemia (CML) is a clonal expansion of the progenitor hematopoietic stem cells arising from the existence of the fusion BCR-ABL oncogene, that represents 15%-20% of the newly diagnosed cases of leukemia patients [ 1 - 2 ]. (jcancer.org)
  • Acute myeloid leukemia (AML) results from the over-proliferation of progenitor cells of the myeloid lineage in the bone marrow. (ghrnet.org)
  • The paradox is that the mutation provides advantage at the stem cell level and progenitor cells, with a disadvantage at the level of hematopoietic precursors. (standardofcare.com)
  • In acute myeloid leukemia (AML), malignant transformation and uncontrolled proliferation of an abnormally differentiated, long-lived myeloid progenitor cell results in high circulating numbers of immature blood cells and replacement of normal marrow by malignant cells. (msdmanuals.com)
  • BCR-ABL oncogene encodes a BCR-ABL tyrosine kinase (TK), which triggers different downstream targets including c-Myc, STAT5 and CrkL, which participated in the control of cell differentiation, proliferation, migration and other cellular behaviors [ 3 - 5 ]. (jcancer.org)
  • In addition to supplying cellular energy, mitochondria are involved in other tasks, such as signaling , cellular differentiation , and cell death , as well as maintaining control of the cell cycle and cell growth . (wikipedia.org)
  • [20] This finding has led to general acceptance of the endosymbiotic hypothesis - that free-living prokaryotic ancestors of modern mitochondria permanently fused with eukaryotic cells in the distant past, evolving such that modern animals, plants, fungi, and other eukaryotes are able to respire to generate cellular energy . (wikipedia.org)
  • 2021). The antiandrogen enzalutamide downregulates TMPRSS2 and reduces cellular entry of SARS-CoV-2 in human lung cells . (essex.ac.uk)
  • The defining functional feature of NK cells remains their intrinsic ability to conduct "natural killing" of cellular targets without prior sensitization. (frontiersin.org)
  • Mitochondria is the central site of cellular energy production and plays an important role in cell survival and death. (biomed.news)
  • Yet, NOX participates in cellular signaling in a cell-intrinsic and -extrinsic manner, e.g., via the release of ROS into the extracellular space. (frontiersin.org)
  • There is clonal proliferation of malignant hematopoietic stem cells, dysregulated cellular differentiation, and compromised tissue function. (standardofcare.com)
  • At that time, glycobiology, which is the study of carbohydrates and their recognition by motif-specific carbohydrate-binding proteins or lectins, lagged far behind the studies that defined the structural and cellular biology of cell death. (nature.com)
  • Our preclinical studies demonstrate that Triphala is effective in inhibiting the growth of human pancreatic cancer cells in both cellular and in vivo model. (biomedcentral.com)
  • Upon an electrochemical switch, growth factor presentation was reversed, which initiated cellular differentiation along the neuronal lineages. (5dok.org)
  • Although all-trans retinoic acid (ATRA)-induced differentiation has transformed acute promyelocytic leukemia (APL) from the most fatal to the most curable hematological disease, resistance to ATRA in high-risk APL patients remains a clinical challenge. (bvsalud.org)
  • Acute myeloid leukemia (AML) is a malignant disease that affects the myeloid lineage and progresses from normal to pre-leukemic and leukemic stages due to the accumulation of mutations over time. (oncotarget.com)
  • Further flow cytometric analysis demonstrates that apoptotic ATRA-NB4 cells release CD14(+)apo-MP in an idarubicin dosage-dependent manner. (bvsalud.org)
  • Applications Tested: This 61D3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
  • However, the role of CD14(+) microparticles (MPs) derived from apoptotic neutrophils (apo-MP) during the phagocytic process is not clear. (bvsalud.org)
  • In this study, we investigated the role of CD14(+)apo-MP in the cell-cell interaction during the phagocytic process of apoptotic cells by viable ATRA-NB4 cells. (bvsalud.org)
  • We firstly demonstrate that CD14 expression and phagocytic activity of NB4 cells were upregulated simultaneously after ATRA treatment in a time-dependent manner, and both were significantly enhanced via concurrent lipopolysaccharide treatment. (bvsalud.org)
  • The phagocytic activity of ATRA-NB4 cells and lipopolysaccharide-treated ATRA-NB4 cells were both significantly attenuated by pre-treating cells with an antibody specific to either CD14 or TLR4. (bvsalud.org)
  • Both CD14 expression and the phagocytic activity of viable ATRA-NB4 cells were significantly enhanced after incubation with apo-MP harvested from apoptotic ATRA-NB4 cells, and the apo-MP-enhanced phagocytic activity was significantly attenuated by pre-treating apo-MP with an anti-CD14 antibody before incubation with viable cells. (bvsalud.org)
  • We conclude that CD14(+)apo-MP derived from apoptotic ATRA-NB4 cells promotes the phagocytic activity of viable ATRA-NB4 cells in engulfing apoptotic cells. (bvsalud.org)
  • Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (blue histogram) or Anti-Human CD14 PerCP-eFluor® 710 (purple histogram). (thermofisher.com)
  • CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. (thermofisher.com)
  • Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. (thermofisher.com)
  • Mechanistically, the cell differentiation mediated by JOA may be originated from the inhibition of BCR-ABL/c-MYC signaling in CML cells expressing wild-type BCR-ABL and BCR-ABL-T315I. (jcancer.org)
  • Expression of VASP, p-VASP, Zyxin and p-Zyxin increases during megakaryocyte differentiation and VASP inhibition affects this differentiation through reduced CD61 expression in VASP depleted cells. (unicamp.br)
  • The non-linear relationship between cytotoxicity and dosage points toward proliferation arrest and differentiation in response to LSD1 inhibition. (nature.com)
  • These results revealed a potent proliferation block and induction of differentiation in non-DS-AMKL and ML-DS samples, however, the therapeutic efficacy of LSD1 inhibition may be limited by its non-linear dose-response relationship. (nature.com)
  • Accordingly, pre-treatment with 350 nM LSD1 inhibitor for 3 days followed by exposure to serial dilutions of ruxolitinib led to synergistic growth inhibition in non-DS-AMKL and ML-DS cell lines (Supplementary Fig. 4 ), as well as in all ML-DS patient samples (Fig. 1A ). (nature.com)
  • Sleeping Elegance is far more susceptible to more than expression inhibition than piggyBac and Tol2, the cargo capacity of Sleeping Attractiveness is constrained, and contrary to Tol2 and piggyBac that Inhibitors,Modulators,Libraries are lively in all mamma lian cell kinds tested, Sleeping Elegance display cell form dependent exercise. (p450inhibitors.com)
  • Inhibition of IRAK4-L abrogates leukaemic growth, particularly in AML cells with higher expression of the IRAK4-L isoform. (cancer-genetics.org)
  • Based on their functional outcome, co-signaling molecules can be divided as co-stimulators and co-inhibitors, which positively and negatively control the priming, growth, differentiation and functional maturation of a T-cell response. (biomedcentral.com)
  • M cell maturation and cDC activation determine the onset of adaptive immune priming in the neonatal Peyer's patch. (costalab.org)
  • Abstract: VASP (vasodilator-stimulated phosphoprotein) and Zyxin are actin regulatory proteins that control cell-cell adhesion. (unicamp.br)
  • gene rearrangementsMBDmethyl\CpG\binding site proteinMeCP2methyl CpG binding proteins\2NSGNOD.Cg\PrkdcscidIl2rgtm1Wjl/SzJqRT\PCRquantitative genuine\time PCRSOCSsuppressor of cytokine signalingT\ALLT\cell lineage severe lymphoblastic leukemiaThT\helperTSATrichostatin A 1. (thetechnoant.info)
  • We are investigating both known cell death regulators as well as searching for novel proteins that control cell death and chemosensitivity. (gla.ac.uk)
  • A substantial portion of the regulatory interactions in the higher eukaryotic cell are mediated by simple sequence motifs in the regulatory segments of genes and (pre-)mRNAs, and in the intrinsically disordered regions of proteins. (biomedcentral.com)
  • Cell deaths were determined by Trypan blue assay and flow cytometry, and related proteins were characterized by western blot. (biomedcentral.com)
  • Glycans, either alone or complexed with glycan-binding proteins, can deliver intracellular signals or control extracellular processes that promote initiation, execution and resolution of cell death programs. (nature.com)
  • Herein, we review the role of glycans and glycan-binding proteins as essential components of the cell death machinery during physiologic and pathologic settings. (nature.com)
  • However, we know less about the biological effects of IL-21 and its signaling mechanisms in leukemia cells and monocytes. (intechopen.com)
  • We have demonstrated that adherence and release of monocytes from a plastic surface drives their differentiation into immature dendritic cells (DC,) that can mature further during overnight incubation in the presence of apoptotic malignant T cells. (biomedcentral.com)
  • It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines. (lonza.com)
  • However, in mice, in vivo cell fusion between MG and adult stem cells forms hybrids that can partially regenerate ablated neurons.We used organotypic cultures of human retina and preparations of dissociated cells to test the hypothesis that cell fusion between human MG and adult stem cells can induce neuronal regeneration in human systems. (ibecbarcelona.eu)
  • Using biologically relevant elastic substrates to study cell function in vitro has proven beneficial, as the in vivo microenvironment usually is much softer than rigid plastic dishes. (5dok.org)
  • X-VIVO 10 - designed to support the generation of LAK cells in a serum-free environment. (lonza.com)
  • [1] pl. mitochondria ) is an organelle found in the cells of most eukaryotes , such as animals , plants and fungi . (wikipedia.org)
  • Mitochondria have a double membrane structure and use aerobic respiration to generate adenosine triphosphate (ATP), which is used throughout the cell as a source of chemical energy . (wikipedia.org)
  • Some cells in some multicellular organisms lack mitochondria (for example, mature mammalian red blood cells ). (wikipedia.org)
  • The number of mitochondria in a cell can vary widely by organism , tissue , and cell type. (wikipedia.org)
  • A mature red blood cell has no mitochondria, [17] whereas a liver cell can have more than 2000. (wikipedia.org)
  • In response to apoptotic stimulus, permeabilized mitochondria release cytochrome c into the cytoplasm, where cytochrome c forms an apoptosome with Apaf-1 and caspase-9 and triggers the caspase cascade. (biomedcentral.com)
  • Interleukin-21 (IL-21) is produced by activated T cells and it plays many diverse roles by regulating the functions of normal and abnormal cells. (intechopen.com)
  • Such immune dysfunction may be due to a disorder in thymic output function (in particular in young patients), which results in a lower level of naive T-cells in the peripheral blood available for an immune response to the proliferation and abnormal expression of the T cell receptor (TCR) repertoire. (biomedcentral.com)
  • The clonal outgrowth of mutant stem cells favors and abnormal hematopoietic stem cell niche. (standardofcare.com)
  • A leukapheresis harvest containing the clonal, leukemic expansion of malignant CD4 + T cells was obtained from the blood of patients with cutaneous T cell lymphoma (CTCL). (biomedcentral.com)
  • Cutaneous T cell lymphoma (CTCL) is a malignant expansion of mature, clonal CD4 T cells with an affinity for epidermal localization [ 1 ]. (biomedcentral.com)
  • Two decades later, pioneering studies suggested that lectin-like molecules constitutively expressed on the surface of macrophages can selectively recognize changes on glycans decorating the surface of apoptotic thymocytes, 4 , 5 although these studies likewise did not provide substantial insight into the mechanisms by which lectin-glycan interactions regulate cell death. (nature.com)
  • The monocyte fraction was simultaneously activated by column passage, re-added to the apoptotic CTCL cells and co-cultured overnight. (biomedcentral.com)
  • Cells in the monocyte gate were used for analysis. (thermofisher.com)
  • Aberrant expression of a third marker such as Thy-1/CD90 negativity, expression of CLL-1 and IL-3R (CD123), intermediate levels of aldehyde dehydrogenase and co-expression of common chromosomal translocation may be used to distinguish from normal hematopoietic stem cells. (ghrnet.org)
  • JOA displayed the activity of inhibiting the BCR-ABL and promoted differentiation of not only imatinib -sensitive but also imatinib -resistant cells with BCR-ABL mutation, which could become a potent lead compound to overcome the imatinib -resistant induced by inhibitors of BCR-ABL tyrosine kinase in CML therapy. (jcancer.org)
  • 416, a potent DHODH inhibitor previously obtained in our group, inhibited the occurrence of APL in cells and model mice. (bvsalud.org)
  • HDACi-mediated differentiation therapy is a potent and molecularly rational treatment strategy in t(8;21) AML. (ashpublications.org)
  • Drugs targeting diseased tissues or cells can significantly improve the efficacy of drugs. (biomed.news)
  • Due to the different sources and potential paracrine effects, studying the effect of ROS on certain cell types and tissues is rather complex. (frontiersin.org)
  • How do C-type lectins tailor adaptive immunity following phagocytosis of apoptotic cells? (nature.com)
  • Furthermore, there are several ways by which exosomes are taken up by recipient cells: receptor- or lipiddraft-mediated endocytosis, phagocytosis, macropinocytosis, or fusion with the plasma membrane of a target cell, For transportation, exosomes mainly originate from multivesicular bodies (MVB's) in the cells which are produced by the invagination of endosomal limiting membrane (Pfrieger et al . (bbrc.in)
  • Hypoxia-selective macroautophagy and cell survival signaled by autocrine PDGFR activity. (gla.ac.uk)
  • Understanding the function of lectin-glycan recognition systems in cell death will facilitate the implementation of novel therapeutic strategies aimed at controlling unbalanced cell proliferation and survival in several pathologic conditions. (nature.com)
  • Exposure of Capan-2 cells to the aqueous extract of Triphala for 24 h resulted in the significant decrease in the survival of cells in a dose-dependent manner with an IC50 of about 50 μg/ml. (biomedcentral.com)
  • Hematological malignancies, which include lymphoid, myeloid, histiocytic, and mast cell neoplasms, are a heterogeneous group of diseases of diverse incidence, pathogenesis and prognosis (Kornblau et al . (bbrc.in)
  • There is compelling clinical and experimental evidence to suggest that natural killer (NK) cells play a critical role in the recognition and eradication of tumors. (frontiersin.org)
  • However, a class of important immune-modulators is conspicuously absent: agents that utilize the power of innate immune cells to eradicate tumors. (frontiersin.org)
  • A myelodysplastic syndrome (MDS) is one of a group of cancers in which immature blood cells in the bone marrow do not mature, and as a result, do not develop into healthy blood cells. (wikipedia.org)
  • Some types of MDS cause an increase in the production of immature blood cells (called blasts), in the bone marrow or blood. (wikipedia.org)
  • The different types of MDS are identified based on the specific characteristics of the changes in the blood cells and bone marrow. (wikipedia.org)
  • The prognosis depends on the type of cells affected, the number of blasts in the bone marrow or blood, and the changes present in the chromosomes of the affected cells. (wikipedia.org)
  • We describe here the absence of p-VASP ser157 in CML bone marrow cells, in contrast to p-VASP ser157 expression in healthy donors. (unicamp.br)
  • Individual bone marrow Compact disc34+ cells had been bought from Stemcell Technology? (thetechnoant.info)
  • Criteria for diagnosis of MDS consist of anemia, thrombocytopenia, or neutropenia that persist for six months or longer, dysplasia greater than 10% in at least one bone marrow cell lineage, and MDS associated clonal cytogenetic abnormalities or molecular markers. (standardofcare.com)
  • When the bone marrow hematopoietic cells are mostly clonally derived cells, the disease is clinically manifested as cytopenia and morphologic dysplasia. (standardofcare.com)
  • The outcome of this decision-making process is cell state dependent and, consequently, requires the integration of vast amounts of information that is encoded in the local abundance and functional state of a multitude of biomolecules acting as cell state sensors and transmitters. (biomedcentral.com)
  • T-cell anergy is defined as the state in which T-cells fail to respond to previously encountered antigenic stimulation by functional APCs. (biomedcentral.com)
  • In addition, PD-1/PD-L1 interactions contribute to functional T-cell impairment, which fails to elicit minimal residual disease and may be related to leukemia relapse. (biomedcentral.com)
  • The emergence of functional studies on animal lectins during the 1990s has provided the appropriate framework to better understand their roles in cell death. (nature.com)
  • In addition, the low cytotoxicity of T-cells may be related to the high expression level of inhibitory molecules including programmed death-1 (PD-1), LAG-3 and NKG2A in CD8+T cells [ 11 ]. (biomedcentral.com)
  • BeadChip microarray and gene ontology analysis revealed that RUNX1‑RUNX1T1 and MAPK1 suppression reduced the proliferation rate of the t(8;21) cells with deregulated expression of several classical positive regulator genes that are otherwise known to enhance cell proliferation. (prinsesmaximacentrum.nl)
  • RUNX1‑RUNX1T1 suppression supported myeloid differentiation by the differential expression of CEBPA, CEBPE, ID2, JMJD6, IKZF1, CBFB, KIT and CDK6, while MAPK1 depletion inhibited the differentiation of t(8;21) cells by elevated expression of ADA and downregulation of JUN. (prinsesmaximacentrum.nl)
  • The role of PD-1 and PD-L1 in T-cell immune suppression and the potential for immunotherapy via blocking PD-1 and PD-L1 in hematological malignancies are also reviewed. (biomedcentral.com)
  • Based on these results, we sought to develop a clinically, practical, rapid means for producing DC loaded with malignant cells. (biomedcentral.com)
  • CTCL cells were purified with a CD3-magnetic bead column where CD3 engagement rendered the malignant T cells apoptotic. (biomedcentral.com)
  • Apoptotic malignant T cells were avidly engulfed by the phagocytic transitioning DC. (biomedcentral.com)
  • The addition of supportive cytokines further enhanced the number of DC that contained apoptotic malignant T cells. (biomedcentral.com)
  • We report a simple one-step procedure where maturing DC containing apoptotic malignant T cells can be prepared rapidly for potential use in vaccine immunotherapy. (biomedcentral.com)
  • Malignant transformation usually occurs at the pluripotent stem cell level, although it sometimes involves a committed stem cell with more limited capacity for self-renewal. (msdmanuals.com)
  • Building on these observations, we propose that treatment-induced changes in apoptotic dependency, rather than pretreatment dependencies, will need to be recognized and targeted to realize the precise deployment of these new pharmacologic agents. (ibecbarcelona.eu)
  • Here, we focus on the development of NOX in phagocytic cells, where the so-called respiratory burst in phagolysosomes contributes to the elimination of ingested microorganisms. (frontiersin.org)
  • A hallmark of cancer is cell death evasion, underlying suboptimal responses to chemotherapy, targeted agents, and immunotherapies. (ibecbarcelona.eu)
  • Is there a hallmark 'glycosylation signature' that characterizes the initiation, execution and resolution of cell death programs in physiologic and pathologic settings? (nature.com)
  • We found that JOA at lower concentration significantly inhibited the proliferation of CML cells expressing mutant BCR-ABL (T315I mutation included) and wild-type BCR-ABL, which was due to that JOA induced the cell differentiation and the cell cycle arrest at G0/G1 phase. (jcancer.org)
  • RUNX1‑RUNX1T1 and MAPK1 depletion induced cell cycle arrest at the G0/G1 phase. (prinsesmaximacentrum.nl)
  • Activation of p21 or p16 therefore causes cell cycle arrest. (medscape.com)
  • The p19ARF protein, which is encoded by the same locus as p16, also leads to cell cycle arrest by inhibiting the ability of MDM2 to inactivate TP53. (medscape.com)
  • Moreover, we established a microinjection system for transplanting human retinal organoids to demonstrate hybrid differentiation.We first found that cell fusion occurs between MG and adult stem cells, in organotypic cultures of human retina as well as in cell cultures. (ibecbarcelona.eu)
  • Co-Treatment of Prostate Cancer Cells with Mscs Expressing Trail and Iap Inhibitors Reduce Trail Resistance and Production of Trail-Induced Cytokines. (essex.ac.uk)
  • In the early phases of cancer immune surveillance, NK cells directly identify and lyse cancer cells. (frontiersin.org)
  • Cancer cells are characterised by an elevated metabolic plasticity and enhanced production of reactive oxygen species (ROS), two features acknowledged as hallmarks in cancer, with a high translational potential to the therapeutic setting. (biomed.news)
  • The aim of our group is to understand the factors regulating programmed cell death in cancer cells. (gla.ac.uk)
  • Feodoroff M, Mikkonen P, Turunen L, Hassinen A, Paasonen L, Paavolainen L, Potdar S, Murumägi A, Kallioniemi O, Pietiäinen V. Comparison of two supporting matrices for patient-derived cancer cells in 3D drug sensitivity and resistance testing assay (3D-DSRT). (helsinki.fi)
  • Feodoroff M, Mikkonen P, Arjama M, Murumägi A, Kallioniemi O, Potdar S, Turunen L, Pietiäinen V. Protocol for 3D Drug Sensitivity and Resistance Testing of Patient-Derived Cancer Cells in 384-Well Plates. (helsinki.fi)
  • This approach set the basis for therapeutic strategies aimed at eliminating aberrantly glycosylated cancer cells. (nature.com)
  • In the present study, we demonstrate that Triphala significantly inhibit the proliferation of Capan-2 and BxPC-3 human pancreatic cancer cells. (biomedcentral.com)
  • We have therefore investigated the role of matrix stiffness in Notch signaling in breast cancer cells. (5dok.org)
  • The transfected cells had been subjected to a chromosomal transposition assay to deter mine their transposition action. (p450inhibitors.com)
  • Growth-inhibitory effects of Triphala were evaluated in Capan-2, BxPC-3 and HPDE-6 cells by Sulphoradamine-B assay. (biomedcentral.com)
  • The signaling mechanisms of IL-21 in different cell types have been identified. (intechopen.com)
  • While much is known about biochemical signaling, many of the mechanisms that drive cell outcome in response to biophysical influences remain to be uncovered. (5dok.org)
  • This signaling pathway is a highly conserved cell-to-cell communication system that regulates cell fate in development and disease. (5dok.org)
  • Eph/ephrin signaling is a cell-to-cell communication pathway, which regulates cell migration and proliferation. (5dok.org)
  • Treatment includes induction chemotherapy to achieve remission and postremission chemotherapy (with or without stem cell transplantation) to avoid relapse. (msdmanuals.com)
  • Some people have a history of exposure to chemotherapy (especially alkylating agents such as melphalan, cyclophosphamide, busulfan, and chlorambucil) or radiation (therapeutic or accidental), or both (e.g., at the time of stem cell transplantation for another disease). (wikipedia.org)
  • In the present study, we investigated the therapeutic potential of TRAIL in primary human glioma cells. (aacrjournals.org)
  • I am especially interested in the role of mesenchymal stem cells (MSCs) in this process. (essex.ac.uk)
  • Next, we showed that the resulting hybrids can differentiate and acquire a proto-neural electrophysiology profile when the Wnt/beta-catenin pathway is activated in the adult stem cells prior fusion. (ibecbarcelona.eu)
  • Finally, we demonstrated the engraftment and differentiation of these hybrids into human retinal organoids.We show fusion between human MG and adult stem cells, and demonstrate that the resulting hybrid cells can differentiate towards neural fate in human model systems. (ibecbarcelona.eu)
  • Leukaemia stem cells (LSC) are identified by high CD34 and negative CD38 expression. (ghrnet.org)
  • On the cover: Human induced pluripotent stem cell-derived neuroepithelial-like stem cells stained for actin (green) and DNA (blue). (5dok.org)
  • Additionally, a 11-year prospective cohort study of Japanese inhabitants linked low peripheral-blood NK cell cytotoxicity with increased cancer risk ( 12 ). (frontiersin.org)
  • Peripheral bloodstream mononuclear cells had been isolated from buffy jackets of regular donors (United Bloodstream Providers, Albuquerque, NM, USA) by centrifugation within a Ficoll\Paque (GE Health care, Pittsburgh, PA, USA) thickness gradient. (thetechnoant.info)
  • Clonal hematopoietic stem cell disorders characterized by ineffective hematopoiesis and peripheral cytopenias. (standardofcare.com)
  • Histopathology of peripheral nerve and sympathetic ganglion from a patient with autonomic failure, oat-cell carcinoma of the lung, and positive anti-HU antibody titer. (medscape.com)
  • The 8-MOP intercalates in the DNA of nucleated cells and is cross-linked to adjacent pyrimidine bases by UVA light activation. (biomedcentral.com)
  • NK cell activation and the triggering of effector functions is governed by a complex set of activating and inhibitory receptors. (frontiersin.org)
  • Nascent transformed cells elicit NK cell activation and are eliminated. (frontiersin.org)
  • T-cell activation and dysfunction relies on direct and modulated receptors. (biomedcentral.com)
  • Moreover, blocking activation of RIP-1 by necroptosis inhibitor necrotatin-1 inhibits AIF translocation and partially suppresses AA005-induced cell death in SW620 cells demonstrating that RIP-1 protein may be essential for cell death. (biomedcentral.com)
  • Interleukin-1 receptor-associated kinase 4 (IRAK4) was the dominant alternatively spliced isoform in MDS and AML and is characterized by a longer isoform that retains exon 4, which encodes IRAK4-long (IRAK4-L), a protein that assembles with the myddosome, results in maximal activation of nuclear factor kappa-light-chain-enhancer of B cells (NF-κB) and is essential for leukaemic cell function. (cancer-genetics.org)
  • Our experiments revealed that HUVEC-induced angiogenesis by cocultured NB4 cells was suppressed when melatonin alone or in combination with ATRA was added to the incubation medium. (bvsalud.org)