Proliferation and differentiationProgenitor cellsVitroSurvivalAntigensSelf-renewalHematopoietic StemBone MarrowHSCTSolid organ transplantationPhenotypeChondrogenic differentiationProtocolsAllogeneicTherapeuticOrganEmbryonicCellNeuronsMSCsProteinsHepatocytePatientsHSCsInducesComplicationsNSCsHigh-doseLiverRegenerativeCardiomyocytesVivoAcuteCellularRejectionTissueCellsHumanImmuneSyndrome
Proliferation and differentiation3
- Human bmMSCs were described in the late nineties as well [ 6 ] and at the same time a breakthrough study investigated the expression of typical cell surface markers and the proliferation and differentiation properties of human MSCs in more detail [ 7 ]. (hindawi.com)
- The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
- Hence, in this review, we further focused on the potential bioapplications of graphene-based nanomaterials for the proliferation and differentiation of NSCs. (rsc.org)
Progenitor cells2
- They display differentiation capacities and therefore qualify as multipotent progenitor cells (Figure 1 ). (hindawi.com)
- Transplantation of neural progenitor cells (NPC) is a promising therapeutic strategy for replacing neurons lost after spinal cord injury, but significant challenges remain regarding neuronal integration and functional connectivity. (jneurosci.org)
Vitro9
- Changes in the differentiation potential of MSCs after in vitro expansion were noted and chondrogenic clones especially disappeared early on [ 12 ]. (hindawi.com)
- Although liver failure can be treated via hepatocyte transplantation, it also faces multiple problems comprising the shortage of high-quality hepatocytes sources, rejection of allogeneic transplants, difficulty to expand, and losing hepatic characteristics in vitro [ 7 , 8 ]. (springer.com)
- Different groups have established several protocols to induce the differentiation of MSCs into HLCs in vitro . (springer.com)
- Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
- Reduction of in vitro culture time to prevent differentiation of the basal keratinocytes in the in vitro phase. (technovelgy.com)
- This study demonstrated that SN-iPSCs-imDCs have potential applications in vitro and in vivo for induction of immunotolerance following organ transplantation. (newswise.com)
- This study focused on exploring sinomenine (SN) to promote differentiation of induced pluripotent stem cells (iPSCs) into imDCs (SN-iPSCs-imDCs), and found SN-iPSCs-imDCs with worse donor-specific T-cell stimulatory function, and higher regulatory T-cell proliferative function in vitro and in vivo to induce high immune tolerance. (newswise.com)
- Of the different cells isolated from the submandibular gland, this specific population, Lin - CD24 + c-Kit + Sca1 + , possessed the highest capacity for proliferation, self renewal, and differentiation during serial passage in vitro. (jci.org)
- Since there has been no in vivo application of graphene in neuronal regenerative medicine, we hope that this review can excite further and concentrated investigations on in vivo (and even in vitro ) neural proliferation, stimulation and differentiation of stem cells on biocompatible graphene scaffolds having the potential of degradability for the generation of implantable neuronal networks. (rsc.org)
Survival7
- Moshayedi P, Nih LR, Llorente IL, Berg AR, Cinkornpumin J, Lowry WE, Segura T, Carmichael ST. Systematic optimization of an engineered hydrogel allows for selective control of human neural stem cell survival and differentiation after transplantation in the stroke brain. (duke.edu)
- however, for those with ESRD attributed to diabetes, kidney transplantation affords better survival and rehabilitation than either hemodialysis or peritoneal dialysis. (medscape.com)
- Timing of intra-arterial neural stem cell transplantation after hypoxia-ischemia influences cell engraftment, survival, and differentiation. (anaspec.com)
- We have found a suitable substrate that can be used to enhance neuronal survival after cell transplantation and we expect to publish a research paper in this new method of cell transplantation. (ca.gov)
- Histological measures of NSC survival, migration, and differentiation were taken at 6 and 8 weeks post-transplant. (iospress.com)
- BACKGROUND: This study aimed to assess the outcome of stem cell transplantation (SCT), including overall survival (OS), failure-free survival (FFS) and graft-versus-host disease (GvHD)-free/failure-free survival (GFFS), and to analyze prognostic. (koreamed.org)
- Long-term survival following organ transplantation has improved considerably. (medscape.com)
Antigens1
- Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
Self-renewal5
- Overview on self-renewal or differentiation of stem cells in their respective stem cell niche. (hindawi.com)
- MSCs have the capacity of multiple differentiations and self-renewal and have been proven to be able to differentiate into a series of cell lineages, such as adipocytes, osteoblasts, chondroblasts, and hepatocyte-like cells (HLCs). (springer.com)
- HSCs specifically are defined by their ability for self-renewal and to reconstitute the entire hematopoietic system following transplantation. (stemcell.com)
- Stem cells are defined by their capacity for self-renewal and multilineage differentiation, making them uniquely situated to treat a broad spectrum of human diseases. (articlecity.com)
- A broader interest of our laboratory is to understand the molecular regulation of HSCs both from the perspectives of self-renewal and differentiation, and how these features become altered upon disease-causing or disease-associated perturbations. (lu.se)
Hematopoietic Stem2
- Today, more than 50 years after the first successful bone marrow transplantation, clinical application of hematopoietic stem cells is a routine procedure, saving the lives of many every day. (hindawi.com)
- For example, because hematopoietic stem cells can reconstitute the entire blood system, bone marrow transplantation has long been used in the clinic to treat various diseases. (articlecity.com)
Bone Marrow4
- In experimental and clinical studies unselected mononuclear bone marrow cells as well as specific subpopulations have been used for transplantation. (escardio.org)
- A new study challenges long-held theories of why a common virus -- cytomegalovirus, or CMV -- can reactivate and become a life-threatening infection in people with a compromised immune system, including blood cancer patients undergoing bone marrow transplantation. (sciencedaily.com)
- This is a big deal for the bone marrow transplantation field," said Dr. Geoffrey Hill, the paper's senior co-author and director of Hematopoietic Stem Cell Transplantation at Fred Hutchinson Cancer Research Center. (sciencedaily.com)
- CMV infection is the most common complication of bone marrow transplantation. (sciencedaily.com)
HSCT6
- 30,000 patients with blood-related malignancies receive HDC, which, if the response is satisfactory, could subsequently be followed by hematopoietic stem cell transplantation (HSCT). (bdbiosciences.com)
- BACKGROUND: Hematopoietic stem cell transplantation (HSCT) is a well-established treatment modality for a variety of diseases. (koreamed.org)
- PURPOSE: To identify the symptoms and signs of patients with dry eye disease (DED) after allogenic hematopoietic stem cell transplantation (HSCT) and analyze the correlations between these signs and symptoms. (koreamed.org)
- BACKGROUND AND OBJECTIVES: Cells of innate immunity normally recover in the first weeks to months after allogenenic hematopoietic stem cell transplantation (allo-HSCT). (koreamed.org)
- BACKGROUND: Allogeneic hematopoietic stem cell transplantation (allo-HSCT) can cure leukaemia. (koreamed.org)
- 1 line of systemic therapy in case of participants ineligible for high-dose chemotherapy and autologous Hematopoietic stem cell transplantation (HSCT). (who.int)
Solid organ transplantation5
- Solid organ transplantation is an important therapeutic option for children with a variety of end stage diseases. (stanford.edu)
- High-dimensional profiling of pediatric immune responses to solid organ transplantation. (stanford.edu)
- Low bone mass is extremely common among patients awaiting solid organ transplantation. (medscape.com)
- A large and rapid decrease in bone mineral density (BMD) occurs within the first year following virtually all forms of solid organ transplantation. (medscape.com)
- In addition to these traditional risk factors, other risk factors are associated with solid organ transplantation. (medscape.com)
Phenotype1
- EE selectively increased cell differentiation into MSN phenotype and promoted host-to-graft connectivity. (lu.se)
Chondrogenic differentiation2
- Promotes osteogenic, adipogenic, and chondrogenic differentiation of human mesenchymal cells (Jaiswal et al. (stemcell.com)
- Promotes osteogenic, adipogenic, and chondrogenic differentiation of mouse mesenchymal cells (Tropel et al. (stemcell.com)
Protocols3
- An accurate measurement of CD34 is critical for dose requirement protocols in stem cell transplantation. (bdbiosciences.com)
- Protocols for hepatocyte differentiation from iPS cells have been studied. (spandidos-publications.com)
- To use this knowledge to improve beta cell differentiation protocols. (lu.se)
Allogeneic4
- Cytomegalovirus reactivation is still a critical concern following allogeneic hematopoietic cell transplantation, and cellular immune reconstitution of cytomegalovirus-specific cytotoxic T-cells is necessary for the long-term control of cytomegalovirus reactivation after allogeneic hematopoietic cell transplantation. (nature.com)
- 3 Autologous stem cell transplantation almost exclusively uses peripheral blood, while for allogeneic SCT, T-lymphocytes in the peripheral blood could pose some danger. (bdbiosciences.com)
- The difference between TNF-α values induced by syngeneic versus allogeneic grafts at day 11 after transplantation reached statistical significance. (jci.org)
- C ) 2D-DIGE of lysates obtained from nontransplanted and allogeneic skin transplants (day 7 after transplantation) and labeled with cy2 and cy5 N-Hydroxysuccinimide ester dyes, respectively. (jci.org)
Therapeutic3
- The aims of this article are to review the current knowledge regarding therapeutic mechanisms of mesenchymal stem cells in acute liver failure, to discuss recent advancements in preclinical and clinical studies in the treatment of mesenchymal stem cells, and to summarize the methodological improvement of mesenchymal stem cell transplantation in treating liver failure. (springer.com)
- Summary of Research Progress: Our research aims to identify the optimal culture conditions and the best hESC lines for the derivation of nerve lineage cells in therapeutic cell transplantation. (ca.gov)
- Plasma cell biology: Foundations for targeted therapeutic development in transplantation. (cincinnatichildrens.org)
Organ5
- The ideal treatment option for terminally ill patients is organ transplantation. (frontiersin.org)
- To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
- Organ transplantation is the ultimate treatment option for patients suffering from refractory diseases. (frontiersin.org)
- Depending on the patient's medical condition, a refractory disease patient also requires an on-time selective option, such as less invasive cellular therapy options or curative organ transplantation that can function immediately after transplantation. (frontiersin.org)
- Even before transplantation, bone homeostasis may be adversely influenced by the disease process or diseased organ itself (eg, liver, lung, or kidney failure). (medscape.com)
Embryonic1
- Promotes differentiation of mature hepatocytes from mouse and human embryonic stem (ES) cells (Cai et al. (stemcell.com)
Cell29
- These methods and results can help us to better understand immune reconstitution following hematopoietic cell transplantation, leading to future studies on the clinical application of adoptive T-cell therapies. (nature.com)
- Gurdon's recent research has focused on analysing intercellular signalling factors involved in cell differentiation, and on elucidating the mechanisms involved in reprogramming the nucleus in transplantation experiments, including the role of histone variants, and demethylation of the transplanted DNA. (wikipedia.org)
- Toward this goal, we propose to compare the behavior of nerve cell differentiation in multiple lines of hESCs in one laboratory setting. (ca.gov)
- We will further characterize molecular changes during directed cell differentiation and identify the cells that exhibit a pattern of DNA modification, namely DNA methylation, similar to primary neural cells in human brain. (ca.gov)
- Finally, cell transplantation in a mouse stroke model will be used to study the mechanisms and efficacy of different types of hESC-derived neural cells in neural repair. (ca.gov)
- We are now ready to compare the property of different lines of nerve cells such as the efficiency of nerve cell differentiation and the preferential production of specific nerve cells in culture. (ca.gov)
- By engineering stem cells carrying different levels of methylation, we aim to find the optimal levels of DNA methylation for efficient nerve cell differentiation. (ca.gov)
- Finally, we also made excellent progress on the procedure of cell transplantation. (ca.gov)
- Stem cell transplantation is performed after high-dose chemotherapy (HDC) to restore a cancer patient's blood and immune cell production capacity. (bdbiosciences.com)
- 1 Improvements in transplantation techniques, including the wider use of cell selection, have contributed to a significant reduction in the morbidity and mortality associated with conventional transplantation. (bdbiosciences.com)
- Because the beta cells in the pancreatic islets are selectively destroyed by an autoimmune process in type 1 diabetes , clinicians and researchers are actively pursuing islet transplantation as a means of restoring physiological beta cell function, which would offer an alternative to a complete pancreas transplant or artificial pancreas . (wikipedia.org)
- A correlation exists between MRD and the risk of relapse in patients with de novo AML after induction therapy and before hematopoietic cell transplantation. (medpagetoday.com)
- The idea of myocardial regeneration with stem cell transplantation after myocardial infarction receives tremendous interest. (escardio.org)
- Several preliminary reports have demonstrated that local stem cell transplantation in patients with acute myocardial infarction is safe and may lead to improved myocardial function and perfusion. (escardio.org)
- Scholars@Duke publication: Differentiation characteristics of newly established medulloblastoma cell lines (D384 Med, D425 Med, and D458 Med) and their transplantable xenografts. (duke.edu)
- It was hypothesized that the addition of glucose and galactose promoted hepatocyte differentiation of iPS cell. (spandidos-publications.com)
- Serial transplantations of this stem cell population into the submandibular gland of irradiated mice successfully restored saliva secretion and increased the number of functional acini. (jci.org)
- This involves placing stem cell clusters into a special medium that induces the spontaneous formation of undeveloped neurons, followed by their differentiation into retinal cells. (labmanager.com)
- We implemented automated liquid change during retinal differentiation and showed it had no negative effect on cell specialization," commented Evgenii Kegeles of the Genome Engineering Lab at MIPT. (labmanager.com)
- One of our goals in this research has been to scale up cell differentiation to enable a high-throughput tissue production for drug tests and cell transplantation experiments. (labmanager.com)
- Numbers matter: The automation empowered us to produce trillions of retinal neurons for transplantation and we are excited to see the translation of our approach into routine cell manufacture," commented Petr Baranov from the Schepens Eye Research Institute of Massachusetts Eye and Ear. (labmanager.com)
- Although graphene/stem cell-based tissue engineering has recently emerged and has promisingly and progressively been utilized for developing one of the most effective regenerative nanomedicines, it suffers from low differentiation efficiency, low hybridization after transplantation and lack of appropriate scaffolds required in implantations without any degrading in functionality of the cells. (rsc.org)
- Then, various stimulation techniques (including electrical, pulsed laser, flash photo, near infrared (NIR), chemical and morphological stimuli) which have recently been implemented in graphene-based stem cell differentiations were reviewed. (rsc.org)
- CONCLUSIONS: Sox9 is a master regulator of mucous neck cell differentiation during gastric development. (bvsalud.org)
- Gastric mucosal injuries include focal and diffused injuries, which do and do not change the cell differentiation pattern. (bvsalud.org)
- Recent news of an impending clinical cell transplantation trial in Parkinson's disease using parthenogenetic stem cells as a source of donor tissue have raised hopes in the patient community and sparked discussion in the research community. (lu.se)
- Based on discussions held by a global collaborative initiative on translation of stem cell therapy in Parkinson's disease, we have identified a set of key questions that we believe should be addressed ahead of every clinical stem cell-based transplantation trial in this disorder. (lu.se)
- During extracellular matrix formation, fibronectin-1 helps individual cells expand (spread) and move (migrate) to cover more space, and it also influences cell shape and maturation (differentiation). (medlineplus.gov)
- We have developed and applied FACS-based high-resolution cell fractionation procedures to map the differentiation processes from HSCs. (lu.se)
Neurons2
- This study optimized conditions for the differentiation of hONPs towards dopaminergic neurons. (scirp.org)
- With this knowledge at hand, we become better at controlling the differentiation of human pluripotent cells to subtype-specific neurons which can be used for disease modeling, drug screening, and transplantation, leading to new treatments for several types of brain disorders. (lu.se)
MSCs2
- Therefore, MSCs may help resolve issues restricting the application of hepatocyte transplantation. (springer.com)
- The differentiation, proliferation, prognosis, and safety of MSCs for in vivo therapy remain controversial. (molvis.org)
Proteins1
- Analysis of transplanted lysates identified haptoglobin as one of the proteins upregulated during transplantation. (jci.org)
Hepatocyte2
- After two days of culture in HSM, the expression levels of α-fetoprotein (AFP) and albumin, which are hepatocyte markers, were upregulated, suggesting that hepatocyte differentiation was initiated ( 19 ). (spandidos-publications.com)
- The HSM was modified into a hepatocyte differentiation inducer (HDI). (spandidos-publications.com)
Patients13
- Although kidney transplantation has become a mainstay in the long-term management of many patients with renal failure , the procedure can be associated with complications such as neurologic, immunologic, infectious, vascular, and urologic. (medscape.com)
- The need for renal transplantation has grown as the number of patients with diabetes has increased. (medscape.com)
- Ploussard et al studied the use of systematic aortofemoral color Doppler ultrasound (DUS) in recipients of a first renal transplantation and found that of 84 patients presenting with a normal preoperative physical arterial examination, 12 (14.3%) had an abnormal DUS, revealing atherosclerosis but not stenosis. (medscape.com)
- Similarly, the transplantation of other tissue-specific stem cells, such as stem cells isolated from epithelial and neural tissues, can treat mouse disease models and human patients in which epithelial and neural cells are damaged. (articlecity.com)
- Hepatocytes derived from iPS cells are suitable for transplantation into patients with liver insufficiency and for use in toxicity tests ( 2 ). (spandidos-publications.com)
- RÉSUMÉ La présente étude a examiné les connaissances et la compréhension actuelles des patients en matière de substitution par des génériques. (who.int)
- À notre avis, la substitution par des génériques ne doit pas être mise en œuvre de manière aléatoire en raison de l'incertitude et des faibles connaissances des patients. (who.int)
- Because patients often wait 2 or more years before transplantation, this represents an opportunity to prevent further bone loss and to help restore what may already have been lost. (medscape.com)
- Osteoporosis is very common among patients awaiting lung transplantation. (medscape.com)
- [ 7 ] Prior to transplantation, BMD was decreased at all sites measured, and 35% of patients awaiting transplant had established osteoporosis, as defined by the World Health Organization (WHO). (medscape.com)
- Moreover, following lung transplantation, nearly three quarters (73%) of patients were at or below the fracture threshold. (medscape.com)
- Therefore, a central goal of diabetes research is to regenerate pancreatic beta cells or produce large numbers of functional beta cells for transplantation into diabetic patients. (lu.se)
- 481 pour 1 000 patients en 2005. (who.int)
HSCs2
- This pharmacologically induced egress of HSCs into peripheral blood, called mobilization, is utilized as the preferred strategy for generating HSCs for transplantation. (bdbiosciences.com)
- In experimental settings, the ability of human HSCs to reconstitute hematopoiesis is measured by transplantation into genetically immunocompromised mice (e.g. (stemcell.com)
Induces2
- After transplantation, MyD88 induces DC maturation as well as the production of inflammatory mediators, such as IL-6 and TNF-α. (jci.org)
- BACKGROUND & AIMS: Acute and chronic gastric injury induces alterations in differentiation within the corpus of the stomach called pyloric metaplasia. (bvsalud.org)
Complications2
- The only curative treatment for ALF is liver transplantation, but there are many restrictions on the application of liver transplantation because of financial considerations, a shortage of donor livers, and immunosuppression-related complications [ 3 ]. (springer.com)
- The use of ASCs in regenerative medicine and tissue engineering research has important advantages in comparison with ESCs, since there are no ethical complications and the process of differentiation of these cells is better controlled. (bvsalud.org)
NSCs1
- Among various stem cells, neural stem cells (NSCs) and their neural differentiation on graphene have attracted a lot of interest, because graphene-based neuronal tissue engineering can promisingly realize the regenerative therapy of various incurable neurological diseases/disorders and the fabrication of neuronal networks. (rsc.org)
High-dose1
- After transplantation, high-dose steroid therapy and immunosuppression further promote bone loss and fracture development. (medscape.com)
Liver2
- The only curative treatment for acute liver failure is liver transplantation, but there are many restrictions on the application of liver transplantation. (springer.com)
- noma and liver transplantation. (who.int)
Regenerative1
- 8,9 Because dental stem cells (DSCs) are easy to obtain and present a great potential of differentiation, there has been a growing interest in their use in regenerative medicine for treatment of various human diseases. (bvsalud.org)
Cardiomyocytes1
- Moreover, it seems that paracrine action of skeletal myoblasts facilitate neighboring cardiomyocytes to maintain their replicative potential and/or stimulate differentiation of native cardiac stem cells (8,9). (escardio.org)
Vivo1
- These cells can be tested in in-vivo transplantation studies into NOD mice. (lifemapsc.com)
Acute1
- Here, we show that haptoglobin, an acute phase protein, is an initiator of this MyD88-dependent inflammatory process in a mouse model of skin transplantation. (jci.org)
Cellular1
- It is used both by hematopoietic researchers and clinical labs to assess the potency of transplantation units of cord blood (CB) and hematopoietic cellular therapy products. (stemcell.com)
Rejection2
- Beyond GWAS—Could Genetic Differentiation within the Allograft Rejection Pathway Shape Natural Immunity to COVID-19? (mdpi.com)
- However, tissue rejection following ESCs derivatives transplantation greatly hinders its application. (benthamscience.com)
Tissue1
- By semi-automating tissue differentiation, researchers from the Moscow Institute of Physics and Technology (MIPT) and Harvard have made the process nearly four times faster, without compromising on quality. (labmanager.com)
Cells2
- The number of viable CD45+/CD34+ cells will determine the quality of the harvested specimen for transplantation. (bdbiosciences.com)
- Stem cells are not specialized and the process of their specialization is called differentiation. (benthamscience.com)
Human1
- Results: Human striatal progenitors survived up to 6 months after transplantation and showed morphological and neurochemical features typical of human MSNs. (lu.se)
Immune1
- A key molecule in this immune pathway is the intracellular TLR signal adaptor known as myeloid differentiation primary response gene 88 (MyD88). (jci.org)
Syndrome1
- Dr. Lai, of the University of Pennsylvania, believes steroids are "very effective" for both the treatment and prevention of differentiation syndrome. (medpagetoday.com)