Differentiate into cells. Medical search. Frequent questions

But we also have provided the first evidence that BMDCs can contribute simultaneously to different encephalic areas through different mechanisms of plasticity - cell fusion for Purkinje cells - among the largest and most elaborately dendritic neurons in the human brain - and differentiation for olfactory bulb interneurons. (eurekalert.org)
show that teratoma formation can be minimized by transplanting the cells into an engineered niche that promotes efficient differentiation. (nature.com)
Scientists at the University of Pennsylvania School of Veterinary Medicine have found a new wrinkle in the developmental biology dogma that cell differentiation occurs irreversibly as stem cells give rise to increasingly specialized types of offspring cells. (innovations-report.com)
Chimeric antigen receptor T (CART) cell therapy targeting the B cell specific differentiation antigen CD19 has shown clinical efficacy in a subset of relapsed/refractory (r/r) diffuse large B cell lymphoma (DLBCL) patients. (frontiersin.org)
Scientists believed that stem cell differentiation was controlled by multiple, highly specific and complex chemical systems, each capable of producing only a limited variety of cells. (rejuvalife.md)
Prior to this discovery, controlling stem cell differentiation often involved direct genetic modification of the cell, which came with a significant risk of the cell becoming cancerous. (rejuvalife.md)
The differentiation of mesenchymal stem cells towards an osteoblastic fate depends on numerous signaling pathways, including activation of bone morphogenetic protein (BMP) signaling components. (medsci.org)
In our previous study, we demonstrated that an agonist of the perioxisome proliferator-activated receptor γ (PPARγ), a master regulator of adipocyte differentiation, stimulates osteoblastic differentiation of cultured human periosteum-derived cells. (medsci.org)
In addition, the PPARγ agonist clearly increased osteogenic differentiation medium-induced BMP-2 upregulation in the periosteum-derived osteoblastic cells at 2 weeks of culture as determined by quantitative reverse transcriptase polymerase chain reaction (RT-PCR), immunoblotting, and immunocytochemical analyses. (medsci.org)
The human NK cell repertoire is functionally diversified through a tightly regulated differentiation process characterized by an early transition from CD56bright to CD56dim NK cells, followed by coordinated changes in expression of inhibitory receptors, including NKG2A and killer cell immunoglobulin-like receptors (KIR). (confex.com)
The acquisition of self HLA class I binding KIRs during NK cell differentiation tunes the cytotoxic potential of NK cells in a process termed education, characterized by increased loading of granzyme B in dense core granules. (confex.com)
Although NK cell differentiation and education are critical determinants of the functional potential of the cell, little is known about how these events shape the migratory behavior of NK cells. (confex.com)
The present results show that peripheral blood NK cell chemokine receptor profile changes in a coordinated fashion during NK cell differentiation and is further influenced by the expression of self-specific KIR. (confex.com)
Interestingly, receptors which expression declines during NK cell differentiation (CCR5, CCR7, and CXCR3) are commonly associated with adaptive T cell responses to viruses, whereas receptors that are upregulated along the differentiation axis (CXCR1, CXCR2, CX3CR1, CMKLR1) are typical for neutrophils and macrophages as a part of the innate immune response. (confex.com)
BioTek's Cytation™ 5 Cell Imaging Multi-Mode Reader was used to monitor spheroids in the inserts during the differentiation process using the brightfield channel, as well as to capture fluorescent images to verify protein marker expression and chondrocyte differentiation. (selectscience.net)
These observations support the view that the division of cells in advanced stages of differentiation may be important in the growth of certain organs and tissues. (rupress.org)
For the differentiation of dendritic cells from mouse bone marrow. (rndsystems.com)
Cytokines produced by DCs can also promote the differentiation of CD4 + T helper cells as part of immune activation. (rndsystems.com)
The CellXVivo ™ Mouse Dendritic Cell Differentiation Kit contains the media and cytokine components to generate immature and mature dendritic cells from mouse bone marrow. (rndsystems.com)
This kit contains the following optimized proteins and reagents to drive the efficient differentiation of mouse monocytes into dendritic cells. (rndsystems.com)
Mouse PBMCs were differentiated into immature mouse dendritic cells by culturing for 5 days in reagents included in the CellXVivo ™ Mouse Monocyte-derived Dendritic Cells Differentiation Kit. (rndsystems.com)
Immature dendritic cells (left, blue) and TNF-α-treated mature dendritic cells (right, red), derived from bone marrow cells cultured with the CellXVivo ™ Mouse Dendritic Cell Differentiation Kit, were stained for flow cytometry with antibodies for CD11c, B7-2/CD86, MHC class II (I-A/I-E), or CD40 (open histograms). (rndsystems.com)
In addition to osteocyte-like cells, the spheroids consisted of osteoblasts at various differentiation stages surrounded by a rim of cells resembling lining cells. (ntnu.no)
This resemblance to native bone indicates a model system with potential for studying osteocyte-like cell differentiation, cross-talk between bone cells, and the mineralization process in a bonelike structure in vitro without artificial scaffolds. (ntnu.no)
Huang and Chang then looked at the proteins GATA1 and PU.1, transcription factors that normally favor differentiation into red and white blood cells, respectively. (sciencedaily.com)
The key attributes like isolation efficiency, cell yield, colony-forming unit-fibroblast (CFU-F) frequency, phenotypic characteristics, and multi-lineage differentiation capacity were determined for the isolated MSCs from bone marrow specimens. (journalcra.com)
The GATA1 protein is involved in the specialization (differentiation) of immature blood cells. (medlineplus.gov)
Through its activity as a transcription factor and its interactions with other proteins, the GATA1 protein regulates the growth and division (proliferation) of immature red blood cells and platelet-precursor cells (megakaryocytes) and helps with their differentiation. (medlineplus.gov)
The model also predicts that reprogramming the network from a differentiated state, in particular the endoderm state, into a stem cell state, is best achieved by over-expressing Nanog, rather than by suppression of differentiation genes such as Gata-6. (lu.se)

BMP signaling also has a critical role in the processes by which mesenchymal stem cells undergo commitment to the adipocyte lineage. (medsci.org)
Human mesenchymal stem cells (hMSCs) have emerged as a leading candidate in cellular therapies due to their unique properties. (selectscience.net)
mesenchymal stem cells are most commonly used because they are easy to obtain and present no ethical problems. (springer.com)
The aims of this article are to review the current knowledge regarding therapeutic mechanisms of mesenchymal stem cells in acute liver failure, to discuss recent advancements in preclinical and clinical studies in the treatment of mesenchymal stem cells, and to summarize the methodological improvement of mesenchymal stem cell transplantation in treating liver failure. (springer.com)
Adult human bone marrow (BM) is the major source of mesenchymal stem cells (MSC) for cell-based therapies. (journalcra.com)
This study focuses to identify, isolate and characterize the mesenchymal stem cells that are believed to be present in BM. (journalcra.com)
And hence, this study suggests that human bone marrow is a potential source of mesenchymal stem cells that could be differentiated in to ccardiomyocytes and used for cell based therapies for treating various cardiovascular diseases. (journalcra.com)

It appears there is at least a small window where terminally differentiated cells vacillate on which identity to adopt. (innovations-report.com)

In vivo engraftment of stem cell-derived cells into damaged or diseased tissue has many challenges. (nature.com)
When dissociated, they can be co-cultured with SC-like adipose-derived stem cells (ASC), providing a valuable model to study in vitro nerve regeneration and myelination, mimicking the in vivo environment at the injury site. (jove.com)
Previous studies have proven that rodent and human MSCs can differentiate into HLCs in vitro and in vivo , which is one of the mechanisms of MSCs in the treatment of liver failure. (springer.com)
Both cell types are pluripotent in vitro, but only ESCs have proven pluripotency in vivo. (wikipedia.org)
Why Differentiate Dendritic Cells ex vivo ? (rndsystems.com)

The fact that the DNA of a fully differentiated (adult) cell could be stimulated to revert to a condition comparable to that of a newly fertilized egg and to repeat the process of embryonic development demonstrates that all the genes in differentiated cells retain their functional capacity, although only a few are active. (who.int)

For one, if pluripotent stem cells are introduced in numbers large enough for tissue regeneration, there is a high risk of teratomas in an immunodeficient mouse (a standard research model for studying engraftment). (nature.com)
Pluripotent cells are implanted in a hydroxyapatite-coated poly- L -lactic acid scaffold that releases bone morphogenic protein 2. (nature.com)
The implants achieve rapid and efficient repair of a calvarial defect with a very low incidence of teratomas (they are seen in 2 of 42 mice) after injection of 1 million human pluripotent cells. (nature.com)
In this experiment, the researchers grew a human embryonic stem cell line and 2 human-induced pluripotent stem cell lines in a culture system that allowed them to produce large numbers of cells. (pharmaceuticalintelligence.com)
The researchers tested more than 150 combinations of over 70 compounds to figure out a method to produce functional human beta cells from the cultured stem cells which when added in exact combinations over a period of several weeks, they transformed human pluripotent stem cells into beta cells that functioned similarly to normal adult beta cells. (pharmaceuticalintelligence.com)

Pushing mature cells into other lineages may offer a new way to replace cells involved in blood diseases and neurodegenerative disorders such as Alzheimer s disease. (innovations-report.com)
MSCs have the capacity of multiple differentiations and self-renewal and have been proven to be able to differentiate into a series of cell lineages, such as adipocytes, osteoblasts, chondroblasts, and hepatocyte-like cells (HLCs). (springer.com)
The findings also help explain why the process of differentiating stem cells into specific lineages in the laboratory has been highly inefficient. (sciencedaily.com)

In vitro, the mineralization ability of human rotator cuff cells from osteoarthritis donors was assessed by alizarin red or Von Kossa staining. (iasp-pain.org)
In vitro, tenocyte-like cells extracted from the rotator cuff were able to mineralize in osteogenic cultures, and expressed , , and , which are hypertrophic chondrocytes markers. (iasp-pain.org)
In vitro , CD3 + CD8 + CD27 - CD28 - compared to CD3 + CD8 + CD27 + CD28 + CART cells displayed similar CD19 + target cell-specific cytotoxicity, but were hypoproliferative and produced less cytotoxic cytokines (IFN-γ and TNF-α). (frontiersin.org)
Overall, Gln supplementation stimulated cell proliferation in muscle tissue and in vitro in myogenic cell culture, whereas muscle growth regulatory genes were barely altered. (nature.com)
Therefore, we complemented our investigations into the cellular development of the skeletal muscle of neonatal piglets with in vitro studies using a primary porcine myogenic cell culture model with Gln supplementation. (nature.com)
Under special conditions in vitro germ cells can acquire properties similar to those of embryonic stem cells (ESCs). (wikipedia.org)

Therefore, we developed an expression signature composed of genes involved in cell cycle progression (CCP) and tested its utility in prostate cancer. (aacrjournals.org)
Cell culture experiments indicated that Gln could promote cell proliferation in a dose dependent manner, but expression of myogenesis regulatory genes was not altered. (nature.com)
The second way is found in mammals, where germ cells are not specified by such determinants but by signals controlled by zygotic genes. (wikipedia.org)
Instead, it supports the idea that cells differentiate through the collective behavior of multiple genes in a network that ultimately leads to just a few endpoints -- just as a marble on a hilltop can travel a nearly infinite number of downward paths, only to arrive in the same valley. (sciencedaily.com)
Again, they found tremendous variability within the apparently uniform cell population: more than 3,900 genes were differentially expressed (turned "on" or "off") between the low- and high-Sca-1 cells. (sciencedaily.com)
Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)

We demonstrate here that lysophosphatidic acid induces process retraction in central glia-4 cells and oligodendrocyte precursors. (nih.gov)
We further show that NP induces binding of endogenous STAT3 to its cognate sequence within the gfap gene promoter in neuroepithelial cells. (elsevierpure.com)

In this study, researchers grafted bone marrow cells into mutant mice suffering from the degeneration of specific neuronal populations at different ages, then compared them to similarly transplanted healthy controls. (eurekalert.org)

Together with other published results, these observations blur the concept of fixed cell types and provide new insights concerning potential uses for adult stem cells. (innovations-report.com)
Instead, germ cells can arise from somatic cells in the adult, such as the floral meristem of flowering plants. (wikipedia.org)
The nucleus of an adult somatic cell (such as a skin cell) is removed and transferred to an enucleated egg, which is then stimulated with electric current or chemicals to activate cell division. (who.int)
if it implants and the pregnancy goes to term, the resulting individual will carry the same nuclear genetic material as the donor of the adult somatic cell. (who.int)

Conclusions: Our results suggest that the developmental program of cochlear differentiating HCs is refractory to Notch reactivation and that Notch is an upstream regulator of Sox2 and Prox1 in cochlear development. (bath.ac.uk)
This chimeric antigen receptor (CAR) T-cell therapy is indicated for the treatment of relapsed or refractory diffuse large B-cell lymphoma (DLBCL), primary mediastinal large B-cell lymphoma (PMBCL), and follicular lymphoma grade 3B (FL3B). (medscape.com)

We have established cell culture conditions that favored the natural 3D orientation of the bone cells and stimulated the cascade of signaling needed for primary human osteoblasts to differentiate into osteocytes with the characteristically phenotypical dendritic network between cells. (ntnu.no)

In the model organism Drosophila, pole cells passively move from the posterior end of the embryo to the posterior midgut because of the infolding of the blastoderm. (wikipedia.org)

It s possible that by targeting this gene with drugs, we may be able to convert malignant B-cell lymphomas into much less harmful histiocytomas, tumors composed of relatively inactive macrophages. (innovations-report.com)
C: Tumor either Stage IA or Stage IB, but with tumor on the surface of one or both ovaries, or with capsule ruptured, or with ascites containing malignant cells or with positive peritoneal washings. (medscape.com)
or ascites present containing malignant cells or with positive peritoneal washings. (medscape.com)
A worldwide database analysis revealed that younger age, smaller tumor size, early stage, and granulosa cell tumor histologic type appear to be independent prognostic factors for improved survival in patients with malignant sex cord-stromal tumors who undergo lymph node dissection. (medscape.com)

Apart from this, it was observed that those MSCs, when induced to differentiate into cardiomyocytes, they successfully differentiated into cardiomyocyte like cells with flat, wide, myotube like structure with sarcomeric organization, which were later confirmed to be cardiomyocytes by qRT-PCR. (journalcra.com)
Thus, this study shows that MSCs were isolated from human bone-marrow and subsequently differentiated into cardiomyocytes with 100% success rate. (journalcra.com)

Background: Notch signaling is active in mouse cochlear prosensory progenitors but declines in differentiating sensory hair cells (HCs). (bath.ac.uk)
Led by Sui Huang, MD, PhD, a Visiting Associate Professor in the Children's Hospital Boston Vascular Biology Program (now also on the faculty of the University of Calgary), and Hannah Chang, an MD/PhD student in Children's Vascular Biology Program, the researchers examined how blood stem cells "decide" to become white blood cell progenitors or red blood cell progenitors. (sciencedaily.com)
Blood stem cells with low levels of Sca-1 differentiated into red blood cell progenitors seven times more often than cells high in Sca-1 when exposed to erythropoietin, a growth factor that promotes red blood cell production. (sciencedaily.com)

These fibrocytes differentiate into myofibroblasts or adipocytes. (medscape.com)

These changed cells are then called embryonic germ cells. (wikipedia.org)
Background: Recent studies have associated the transcription factors, Oct4, Sox2 and Nanog as parts of a self-regulating network which is responsible for maintaining embryonic stem cell properties: self renewal and pluripotency. (lu.se)
At the core of the network reside Oct4, Sox2 and Nanog, into embryonic stem cells [1,2,3,4,5], have made major inroads which form a self-organized core of the TFs maintaining into stem cell biology. (lu.se)

Although still in its early phases, stem cell research is producing some of the most promising advances in modern medicine, and the potential it may have for disease treatment in the future is simply astonishing. (rejuvalife.md)
A research team from the Genome Institute of Singapore, in collaboration with the Cancer Science Institute of Singapore, discovered that the type of cell that a stem cell will eventually turn into is actually controlled by a single communication system. (rejuvalife.md)
We decided to use the three-dimensional culture of Schwann cells differentiated from endometrial stem cell in fibrin gel. (scirp.org)
Stem cell technology offers a potential technique to repair the ruptured AF by enabling new matrix synthesis at the defect site. (utwente.nl)
How does a stem cell decide what specialized identity to adopt -- or simply to remain a stem cell? (sciencedaily.com)
Further, when those MSCs were analyzed for their specific marker expression using flowcytometry, they were found to be positive for CD29, CD73, CD90, and CD105 and negative for CD34 and CD45 stem cell markers respectively. (journalcra.com)
It provides a framework to explore strategies of reprogramming a cell from a differentiated state to a stem cell state through directed perturbations. (lu.se)
Such an approach is highly relevant to regenerative medicine since it allows for a rapid search over the host of possibilities for reprogramming to a stem cell state. (lu.se)
Citation: Chickarmane V, Peterson C (2008) A Computational Model for Understanding Stem Cell, Trophectoderm and Endoderm Lineage Determination. (lu.se)
A computational model of master regulators that are required for successful reprogramming the dynamics of this core network has revealed that it functions as of a differentiated cell into a cell exhibiting stem cell like a bistable switch, which in the on state, corresponds to all these properties. (lu.se)

Germ cells produce gametes and are the only cells that can undergo meiosis as well as mitosis. (wikipedia.org)
Somatic cells are all the other cells that form the building blocks of the body and they only divide by mitosis. (wikipedia.org)
ii: Differentiate Mitosis and Meiosis, give one example for each type of cell division. (hawkessays.com)

Multicellular eukaryotes are made of two fundamental cell types: germ and somatic. (wikipedia.org)
surrounding somatic cells flatten the 16-cell differentiating cyst, resulting in an increase of the membrane tension of germ cells inside the cyst. (glasp.co)
Somatic-cell nuclear transfer, the technique by which Dolly was created, was first used 40 years ago in research with tadpoles and frogs. (who.int)

This study shows a potential new contribution of bone marrow derived cells following transplantation into the brain, making these cells highly versatile, in their ability to both differentiate into and fuse with endogenous neurons" said Dr. Paul R. Sanberg , coeditor-in-chief of CELL TRANSPLANTATION and distinguished professor of Neuroscience at the Center of Excellence for Aging and Brain Repair, University of South Florida. (eurekalert.org)

Diffuse large B cell lymphoma (DLBCL) represents the most frequent form of non-Hodgkin's lymphoma (NHL). (frontiersin.org)

Calcifications were amorphous areas surrounded by a fibrocartilaginous metaplasia containing hypertrophic chondrocyte-like cells that expressed tissue non-specific alkaline phosphatase (TNAP) and ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), which are two key enzymes of the mineralization process. (iasp-pain.org)
Using this system, called the Nodal/Activin pathway, scientists will be able to control the eventual type of tissue the stem cells will develop into, simply by adjusting the cell's chemical environment. (rejuvalife.md)
Dr. Kian Leong Lee, who led the study, says this finding "paves the way for advanced studies in cell regeneration and tissue repair, which could ultimately lead to its use in personalized medicine, where stem cells from the same patient could be manipulated to make other types of cells that are genetically matched to the donor. (rejuvalife.md)
Critical to the hypothesis is the experimental fact that human endometrial-derived stem cells (hEnSCs) as multipotent accessible source of cells are known as useful cell candidates in the field of nerve tissue engineering. (scirp.org)
These novel differentiated cells in fibrin gel might open new opportunities for the management of cell survival and neurotrophic potential in tissue engineering approaches for nerve repair. (scirp.org)
Surgeons can now detect and differentiate between cancerous cells and normal healthy cells with MasSpec Pen rather than performing a cryosection (frozen section analysis of removed tissue done by pathologist) which can be time consuming while patient has already been cut open. (veritalife.com)
The pen uses mass spectrometry to diagnose cancer cells, it is placed on tissue and secretes a drop of water to moisturise it three seconds. (veritalife.com)
The pen simply recognise and distinguish between normal cell or cancerous cells causing no harm to any tissue but it in fact speeds up the surgical procedures. (veritalife.com)
The Fragility of Cryopreserved Insulin-producing Cells Differentiated from Adipose-tissue-derived Stem Cells. (bvsalud.org)
The aim of our study is to determine whether insulin -producing cells (IPCs) differentiated from adipose- tissue -derived stem cells (ADSCs) can be cryopreserved. (bvsalud.org)

Immature dendritic cells have the phenotype of CD11c + , with intermediate expression of B7-2/CD86, MHC class II, and little to no CD40. (rndsystems.com)
Mature dendritic cells have the phenotype of CD11c + , with increased expression of B7-2/CD86, MHC class II, and CD40. (rndsystems.com)
Previous studies have shown that human adipose stem cells (hASCs) are able to differentiate towards AF phenotype when treated with suitable growth factors. (utwente.nl)
qRT-PCR confirmed the similarity of the differentiated hASCs with AF phenotype. (utwente.nl)
Several markers for AF phenotype (aggrecan, collagen type I and glypican-3) were expressed in both hAFCs and differentiated hASCs. (utwente.nl)
This is the first study that demonstrated that hASCs can be differentiated towards AF phenotype using serum free chondrogenic medium without growth factors. (utwente.nl)

Primordial germ cells, germ cells that still have to reach the gonads (also known as PGCs, precursor germ cells or gonocytes) divide repeatedly on their migratory route through the gut and into the developing gonads. (wikipedia.org)

Specification of primordial germ cells in the laboratory mouse is initiated by high levels of bone morphogenetic protein (BMP) signaling, which activates expression of the transcription factors Blimp-1/Prdm1 and Prdm14. (wikipedia.org)
These cells can grow indefinitely in the laboratory and can differentiate, into any cell type found in the body. (pharmaceuticalintelligence.com)

Dendritic cells (DCs) are key mediators of both innate and adaptive immune responses. (rndsystems.com)
Upon activation, immature dendritic cells mature and increase the expression of class II MHC and co-stimulatory molecules important for effective antigen presentation to naïve T cells. (rndsystems.com)
Opened or reconstituted Mouse Dendritic Cell Base Media, Recombinant Mouse GM-CSF, Recombinant Mouse IL-4, or Recombinant Human TNF-alpha should be stored at 2-8 ° C under sterile conditions for up to 30 days or at -20 ° C to -70 ° C in a manual defrost freezer for up to 3 months. (rndsystems.com)
Morphology of Mouse Dendritic Cells Derived from PBMCs. (rndsystems.com)
Immature dendritic cells exhibit characteristic dendritic cell morphology. (rndsystems.com)
Phenotypic Analysis of Cultured Immature and Mature Mouse Dendritic Cells. (rndsystems.com)
Kit-derived Mature Mouse Dendritic Cells Induce Proliferation of Allogeneic T Cells. (rndsystems.com)
Serial dilutions of TNF-α-treated mature dendritic cells were incubated with allogeneic mouse T cells for 3 days. (rndsystems.com)

Scientists also say this approach offers the potential for converting lymphoma cells that are resistant to treatment into more manageable forms of cancer. (innovations-report.com)
While scientists have been able to change stem cells into insulin-producing cells, these cells don't have markers that indicate they are beta cells, and they aren't responsive to glucose. (pharmaceuticalintelligence.com)

High-resolution cell tracking, particle image velocimetry of flagellar driven flows, and high-speed imaging of flagella on micropipette-held colonies show how, in the context of a recently introduced model for Chlamydomonas phototaxis, an adaptive response of the peripheral cells alone leads to photo-reorientation of the entire colony. (biorxiv.org)

The work also suggests a much more efficient way of differentiating stem cells for therapeutic purposes. (sciencedaily.com)

Wunen proteins are chemorepellents that lead the germ cells away from the endoderm and into the mesoderm. (wikipedia.org)

The age differences in total DNA and DNA concentration indicated that there was a rapid rate of proliferation of parenchymal cells until 25 postnatal days, after which the rate declined rapidly, and that there was a rapid increase in cell size between 18 and 25 days. (rupress.org)
Proliferating, BrdU-positive cells in muscle sections were detected with immunohistochemistry indicating different cell types and decreasing proliferation with age. (nature.com)

In affected individuals, immature red blood cells are unusually shaped and cannot develop into functional mature cells, leading to a shortage of healthy red blood cells. (medlineplus.gov)
To function properly, these immature cells must differentiate into specific types of mature blood cells. (medlineplus.gov)
An abnormal GATA1 protein causes immature red blood cells to undergo a form of programmed cell death called apoptosis. (medlineplus.gov)
A lack of immature red blood cells results in decreased amounts of specialized, mature red blood cells, leading to anemia . (medlineplus.gov)

Blood cell and serum markers, along with clinical data of DLBCL patients who were scheduled for CART cell therapy were evaluated to search for biomarkers predicting CART cell responsiveness. (frontiersin.org)
In this study, we investigate the expression of differentiated Schwann cell markers by exposing of endometrial stem cells with induction media including FGF2/FSK/HRG/RA. (scirp.org)
Using immunocytochemistry, we show that differentiated cells express S100 and P75 markers. (scirp.org)
These cells expressed late osteoblast and osteocyte markers such as osteocalcin (OCN), podoplanin (E11), dentin matrix acidic phosphoprotein 1 (DMP1), and sclerostin (SOST). (ntnu.no)
The cultured beta cells had specific markers that were found on normal beta cells which displayed changes in calcium levels when exposed to glucose and packaged insulin into granules. (pharmaceuticalintelligence.com)

Sex cord-stromal tumors are groups of tumors composed of granulosa cells, theca cells, Sertoli cells, Leydig cells, and fibroblasts of stromal origin, singly or in various combinations. (medscape.com)
A subset of orbital fibroblasts in the orbit can differentiate into mature, lipid-accumulating cells or de novo adipogenesis. (medscape.com)

The objectives of this study were to better characterize the cells and mechanisms involved in depositing apatite crystals in human tendons. (iasp-pain.org)
In this study, we used dorsomorphin, a selective small molecule inhibitor of BMP signaling, to investigate whether BMP signaling is involved in the positive effects of PPARγ agonists on osteogenic phenotypes of cultured human periosteum-derived cells. (medsci.org)
Although further study will be needed to clarify the mechanisms of PPARγ-regulated osteogenesis, our results suggest that the positive effects of a PPARγ agonist on the osteogenic phenotypes of cultured human periosteum-derived cells seem to be dependent on BMP signaling. (medsci.org)
These results show that for the first time, human endometrial stem cells can be differentiated into Schwann cells in 2D and 3D culture. (scirp.org)
Transplanted human pancreatic cells from deceased donors have been successfully used to treat people with type 1 diabetes. (pharmaceuticalintelligence.com)
Human annulus fibrosus cell (hAFCs) cultured in commercial AF growth medium were used as positive control. (utwente.nl)
Human ADSCs were differentiated into IPCs using our two-step protocol encompassing a three-dimensional culture and xenoantigen -free method . (bvsalud.org)
Accurate and reliable measures of CD4+ T-lymphocytes (CD4+ T-cells) are essential to the assessment of the immune system of human immunodeficiency virus (HIV)-infected persons (1-3). (cdc.gov)
Produced by the National Institute of Allergy and Infectious Diseases (NIAID), this illustration depicts the Life Cycle of the Malaria Parasite , differentiating between the parasites' development inside the mosquito vector, and development while inside the human host, specifically, inside the liver hepatocytes, and red blood cells (RBCs) circulating in the blood. (cdc.gov)

To quantify the nuclear region of every cell from a time-series 3D fluorescence microscopic image of living cells, we developed QCANet, a convolutional neural network-based segmentation algorithm for 3D fluorescence bioimages. (nature.com)

We found that the levels of B cells, T cells, and NK cells were similar, IC-expressing CD4 + and CD4 + CD8 + double positive T cells were higher, while CD8 + T cells and CD4 − CD8 − double negative T cells were significantly lower in CRC tumors. (mdpi.com)

In other words, unfaithful cells that have strayed from their original identity can come to their senses and return to the original lineage," Thomas-Tikhonenko said. (innovations-report.com)
The lineage of germ cells is called the germline. (wikipedia.org)
There are two mechanisms to establish the germ cell lineage in the embryo. (wikipedia.org)
Since the germ cell lineage is not established right away by induction, there is a higher chance for mutation to occur before the cells are specified. (wikipedia.org)

Collected lavage cells were differentiated to assess inflammation. (cdc.gov)

ABSTRACT Erythrocyte indices used to differentiate between iron deficiency anaemia (IDA) and thalassaemias have been studied mainly in countries with a high prevalence of IDA or beta-thalassaemias. (who.int)

The researchers have shown that certain mouse cells retain an ability to oscillate between very distinct blood cell types - B-cells and macrophages - long after what has been commonly regarded as the point of no return. (innovations-report.com)
Until recently, it was believed that the various cell types of the blood are generated from stem cells in a controlled but irreversible fashion," said James Hagman, an immunology researcher at the National Jewish Medical and Research Center in Denver who was not involved in this research. (innovations-report.com)
A machine learning algorithm has been trained to distinguish between four cancer cell types. (spie.org)
The other approach is to develop functioning beta cells from stem cells which have the potential to transform into many different cell types. (pharmaceuticalintelligence.com)

Cell Transplant;29: 963689720954798, 2020. (bvsalud.org)

A corner-stone of Weismann's theory is his assumption of nuclear divisions which are differentiating. (dictionary.com)
However, an animal created through this technique would not be a precise genetic copy of the source of its nuclear DNA because each clone derives a small amount of its DNA from the mitochondria of the egg (which lie outside the nucleus) rather than from the donor of cell nucleus. (who.int)

Conversely, when stem cells were exposed to granulocyte--macrophage colony-stimulating factor, which stimulates white blood cell formation, those that were highest in Sca-1 were the most likely to become white cells. (sciencedaily.com)

B-cells arise from a lymphoid progenitor, while macrophages come from a myeloid progenitor. (innovations-report.com)

At Rejuvalife Vitality Institute, Dr. Berger uses a patient's own fat-derived stem cells in fat transfer procedures to promote the viability and longevity of fat grafts. (rejuvalife.md)
Cell counting kit-8 ( CCK-8 ) assay showed that cell viability was decreased in all groups after cryopreservation (P (bvsalud.org)

In order to delineate the homing patterns of distinct NK cell subsets, we used high-dimensional flow cytometry combined with functional assays to map the NK cell chemokine receptor expression and migratory behavior. (confex.com)

In many animals, the germ cells originate in the primitive streak and migrate via the gut of an embryo to the developing gonads. (wikipedia.org)
Unlike animals, plants do not have germ cells designated in early development. (wikipedia.org)
After transport, involving passive movements and active migration, germ cells arrive at the developing gonads. (wikipedia.org)
Recent studies have demonstrated that it is possible to give rise to primordial germ cells from ESCs. (wikipedia.org)
The first way is called preformistic and involves that the cells destined to become germ cells inherit the specific germ cell determinants present in the germ plasm (specific area of the cytoplasm) of the egg (ovum). (wikipedia.org)
In mammals, a few cells of the early embryo are induced by signals of neighboring cells to become primordial germ cells. (wikipedia.org)
This means that they can differentiate in any cell type in the body and thus germ cells. (wikipedia.org)
As germ cells are quiescent and therefore not dividing, they are not susceptible to mutation. (wikipedia.org)
However, more mutation rate data will need to be collected across several taxa, particularly data collected both before and after the specification of primordial germ cells before this hypothesis on the evolution of germ plasm can be backed by strong evidence. (wikipedia.org)
After splitting into two populations, the germ cells continue migrating laterally and in parallel until they reach the gonads. (wikipedia.org)

We therefore examined the roles of oxidative stress and proteasomal inhibition on protein aggregates induction in naïve and neuronally differentiated neuroblastoma SH-SY5Y cells. (unboundmedicine.com)
In contrast, dorsomorphin markedly decreased ALP activity, alizarin red S staining and calcium content in both the cells treated with PPARγ agonist and the cells cultured in osteogenic induction media without PPARγ agonist during the culture period. (medsci.org)
It is speculated that induction was the ancestral mechanism, and that the preformistic, or inheritance, mechanism of germ cell establishment arose from convergent evolution. (wikipedia.org)
Induction of SRY box transcription factor 9 (SOX9) has been shown to occur in response to kidney injury in rodents, where SOX9-positive cells proliferate and regenerate the proximal tubules of injured kidneys. (lu.se)

However, it is not known whether reactivation of NICD in differentiating HCs also interrupts their developmental program and reactivates its downstream targets. (bath.ac.uk)

We suspect that this phenomenon is not limited to B-cells and macrophages in mice. (innovations-report.com)
RBP-J loxp/loxp mice, we demonstrated that ectopic NICD in differentiating HCs caused reactivation of Sox2 and Prox1 in an RBP-J-dependent manner. (bath.ac.uk)
However, when transplanted into mice these cells secreted insulin in response to glucose. (pharmaceuticalintelligence.com)
However, when the cells were transplanted into diabetic mice, abnormally high blood glucose levels lowered. (pharmaceuticalintelligence.com)

Univariate and multivariate regression analyses showed that low levels of differentiated CD3 + CD27 - CD28 - T cells (23.3 ± 19.3% versus 35.1 ± 18.0%) were independently associated with OR. (frontiersin.org)

Conversely, CXCR3 was expressed at lower levels on educated NK cells (C). No difference was observed for CXCR2 expression. (confex.com)

a. 3) Differentiate between the function of the seminiferous tubules and the interstitial cells a. 4) List and locate the glands that contributes to the formation of semen. (newessayhelp.com)
Immunologic tests can detect beryllium sensitization and help clinicians differentiate between chronic beryllium disease and other interstitial lung diseases. (cdc.gov)

The results show that lysophosphatidic acid signalling pathways influence retraction of processes in oligodendrocyte precursors but that this effect changes as oligodendrocytes differentiate. (nih.gov)

Naïve and transfected cells were exposed to oxidative stress induced by rotenone, SIN-I, FeCl(2,) and to proteasomal inhibition by lactacystin. (unboundmedicine.com)
Proteasomal inhibition induced significantly increased alpha-synuclein aggregation in cells expressing mutant alpha-synuclein. (unboundmedicine.com)
Lev N, Melamed E, Offen D. Proteasomal inhibition hypersensitizes differentiated neuroblastoma cells to oxidative damage. (unboundmedicine.com)
The differences in strengths and numbers of synapses between PV+ basket cells and either superficial sublayer or deep sublayer pyramidal cells enables a routing of inhibition from superficial to deep pyramidal cells. (yale.edu)

Moreover, the cells could continue oscillating between B-cells and macrophages indefinitely, as many times as they were relocated. (innovations-report.com)

Inoculated cells were further incubated for 1 hour at 37/C before addition of overlay containing 0.6% agarose (Seakem ME) and 10% heated fetal bovine serum (FBS). (cdc.gov)

The CDC guidelines concerning CD4+ T-cell determinations (33) were first published in the MMWR in 1992 to provide laboratorians with the most complete information about how to measure CD4+ T-lymphocytes in blood from HIV-infected persons by using flow cytometry. (cdc.gov)

Interestingly, the contribution of BMDCs occurred through these two different plasticity mechanisms, which strongly suggests that plasticity mechanisms may be modulated by region and cell type-specific factors," he said. (eurekalert.org)
A robust and accurate algorithm to acquire the 3D positions of the cells would help to reveal the mechanisms of embryogenesis. (nature.com)

A solid tumour is usually removed through primary surgery where the surgeons risks removing some of the attached healthy tissues or causing some damage to non cancerous cells. (veritalife.com)
of cells or tissues) to change from relatively generalized to specialized kinds, during development. (dictionary.com)
In type 2 diabetes, the most common form, tissues in the body lose their sensitivity to insulin, and pancreatic beta cells can't make enough insulin to keep glucose levels in check. (pharmaceuticalintelligence.com)
We investigated the expression of immune checkpoints (ICs) on lymphoid populations within the CRC TME and compared with cells from normal colon tissues using samples from 50 patients with varying disease stages. (mdpi.com)
A decrease in hemoglobin can lead to a shortage of oxygen in cells and tissues throughout the body. (medlineplus.gov)

Tampa, Fla. (Dec, 19 2011) - Bone marrow-derived stem cells (BMDCs) have been recognized as a source for transplantation because they can contribute to different cell populations in a variety of organs under both normal and pathological conditions. (eurekalert.org)
The findings, published in the May 22 issue of Nature, give a glimpse into how that collective behavior works, and show that cell populations maintain a built-in variability that nature can harness for change under the right conditions. (sciencedaily.com)
They began by examining populations of seemingly identical blood stem cells, and found that a cell marker of "stemness," a protein called Sca-1, was actually present in highly variable amounts from cell to cell -- in fact, they found a 1,000-fold range. (sciencedaily.com)

Schwann cells (SCs) have important roles in development, myelination and regeneration in the peripheral nervous system. (scirp.org)
SOX9 expression was found to colocalize with a proportion of so-called scattered tubular cells in the uninjured kidney, a cell population previously shown to be involved in kidney injury and regeneration. (lu.se)

Differentiation29

Mesenchymal stem7

Terminally differentiated1

Vivo5

Fully differentiated1

Pluripotent5

Lineages3

Vitro6

Genes6

Induces2

Neuronal1

Adult4

Refractory2

Osteoblasts1

Drosophila1

Malignant4

Cardiomyocytes2

Progenitors3

Adipocytes1

Embryonic3

Stem cell10

Mitosis3

Somatic3

Neurons1

Diffuse large B1

Tissue10

Phenotype6

Precursor1

Laboratory2

Dendritic8

Scientists2

Particle1

Therapeutic1

Endoderm1

Proliferation2

Immature4

Markers5

Fibroblasts2

Human9

Neural1

Tumors1

Lineage4

Inflammation1

Abstract1

Types4

20201

Nuclear2

Stimulates1

Progenitor1

Viability2

Functional1

Germ cells10

Induction4

Developmental1

Mice4

Versus1

Conversely1

Interstitial2

Oligodendrocyte1

Inhibition4

Indefinitely1

Serum1

Flow cytometry1

Mechanisms2

Tissues5

Populations3

Regeneration2