• We identified differentially expressed genes from 14 case-control human breast cancer gene expression datasets and integrated them with drug-protein networks. (sutterhealth.org)
  • Differentially expressed genes (DEGs) may be responsible for phenotypic differences between various biological conditions. (mdpi.com)
  • DEGs, or Differentially Expressed Genes, are genes whose expression levels show significant differences between two or more conditions or experimental groups. (pharmiweb.com)
  • After identifying Differentially Expressed Genes (DEGs), we delve deeper into their functional significance. (pharmiweb.com)
  • Hypergeometric analysis was used to identify canonical pathways and curated networks having statistically significant enrichment of differentially expressed genes. (urotoday.com)
  • The mHTT and wtHTT groups showed the highest number of differentially expressed genes compared to the BACHD mouse model. (lu.se)
  • In BACHD mice, few hypothalamic genes were differentially expressed compared to age-matched WT controls. (lu.se)
  • Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product that enables it to produce end products, proteins or non-coding RNA, and ultimately affect a phenotype. (wikipedia.org)
  • These products are often proteins, but in non-protein-coding genes such as transfer RNA (tRNA) and small nuclear RNA (snRNA), the product is a functional non-coding RNA. (wikipedia.org)
  • Gene expression in living cells is strictly self-regulated to ensure that the correct amounts of proteins are made at the most appropriate timing and location for maintaining cellular homeostasis. (nature.com)
  • Similarly, while your genes are "fixed", the expression of those genes - the amount of proteins they cause to be made, whether or not they are even switched on or off at all - depends on the "environment," the circumstances surrounding those genes . (marksdailyapple.com)
  • Although non-coding RNAs fail to produce functional proteins, researchers believe that in some cases these RNAs may control gene expression. (sciencedaily.com)
  • Cells regulate the expression of their genes with proteins called transcription factors,' said co-corresponding author Dr. Josephine C. Ferreon, assistant professor of pharmacology and chemical biology and member of the Dan L Duncan Comprehensive Cancer Center at Baylor. (news-medical.net)
  • Gene expression is the chemical pathway from genes to the gene products, such as proteins, that organisms can use. (visionlearning.com)
  • EPFL scientists have just found that members of the DUX family of proteins are responsible for igniting the gene expression program of the nascent embryo. (news-medical.net)
  • The study points to DUX4, and by extension the DUX family of proteins, as the master regulator responsible for kick-starting genome expression at the earliest stage of embryonic life in humans, mouse and probably all placental mammals. (news-medical.net)
  • The fact that the Gimap genes are located together in a tight cluster on RNO4 (and in conserved synteny with many other species), combined with their sequence similarities, suggests the possibility that the proteins carry out similar function. (hindawi.com)
  • Applying the same criterium to the process of gene expression, which also involves many 'intermediate steps', would lead us to the conclusion that no genetic information is transmitted from genes to proteins, which is no less difficult to defend. (cyclingforums.com)
  • Analysis of the pattern of proteins or messenger RNAs (mRNAs) in histological tissue sections is a cornerstone in biomedical research and diagnostics.This typically involves the visualization of a few proteins or expressed genes at a time. (lu.se)
  • In genetics and genomics research, gene expression refers to the process by which information encoded in a gene's DNA sequence is converted into functional proteins or RNA molecules. (pharmiweb.com)
  • We have learned that genes in mammalian cells are transcribed into messenger RNAs (mRNAs), which are to be translated into polypeptides (proteins). (intechopen.com)
  • Our project offers a unique opportunity for Master's student to gain hands-on experience focused on transcriptional regulators with importance in expression of genes of outer membrane proteins that are essential in host-pathogen interaction. (lu.se)
  • While transcription of prokaryotic protein-coding genes creates messenger RNA (mRNA) that is ready for translation into protein, transcription of eukaryotic genes leaves a primary transcript of RNA (pre-RNA), which first has to undergo a series of modifications to become a mature RNA. (wikipedia.org)
  • While researchers once believed that only genes were transcribed into messenger RNA (mRNA), investigators have recently discovered that non-coding DNA is copied into mRNA as well. (sciencedaily.com)
  • Pulmonary OPN relative mRNA expression (peaked at 7 d) and protein levels were induced in wild-type mice following MWCNT exposure. (cdc.gov)
  • Pulmonary cytotoxicity, inflammatory cell influx, and relative mRNA expression of genes related to macrophage function and recruitment (Ccl2, Ccl22, and Arg1) were increased as a result of MWCNT exposure but not affected by OPN deficiency. (cdc.gov)
  • Regulation of gene expression gives control over the timing, location, and amount of a given gene product (protein or ncRNA) present in a cell and can have a profound effect on the cellular structure and function. (wikipedia.org)
  • Regulation of gene expression is the basis for cellular differentiation, development, morphogenesis and the versatility and adaptability of any organism. (wikipedia.org)
  • Gene regulation may therefore serve as a substrate for evolutionary change. (wikipedia.org)
  • Gene regulation can occur at any point in gene expression, from the start of the transcription phase to the translation phase. (nature.com)
  • Due to the complexity of gene regulation, unveiling the complete mechanism of gene regulation has been a long-standing quest. (nature.com)
  • To elucidate the complex mechanism of gene regulation in living cellular systems, it is necessary to closely investigate each phase of gene expression and also to shed light on the cooperative effects between transcription and translation. (nature.com)
  • The aforementioned complex factors have made it difficult to investigate the impact of the DNA length on gene regulation, which has been an unexplored topic. (nature.com)
  • Instead, the non-coding RNA was suspected to play a role in hGH gene regulation. (sciencedaily.com)
  • SCIENCE TRANSLATIONAL MEDICINE Scientists at Massachusetts General Hospital and Harvard Medical School have-for the first time-imaged epigenetic regulation of gene expression in the living human brain. (the-scientist.com)
  • In our proposed project, you would investigate expression regulation in clinically significant bacterium non-typeable Haemophilus influenzae (NTHi). (lu.se)
  • 2022 ) Gene Expression Regulation in Airway Pathogens: Importance for Otitis Media. (lu.se)
  • Recent studies support a role for biotin in cell proliferation, DNA repair, epigenetic gene regulation as well as normal immune function. (medscape.com)
  • Improved access to bioinformatics tools enables scientists to process, manage, and store raw data, annotate sequences to define genes, and quantitate RNA sequence data to get insight into gene expression patterns. (genengnews.com)
  • To identify differing patterns of gene expression, they used RNA sequencing (RNAseq), a technique that reflects how strongly genes are being expressed in a given tissue. (eurekalert.org)
  • Environmental conditions experienced by parents can influence gene expression patterns in their offspring. (umassmed.edu)
  • Once they determined the larger trends in gene expression following the damage, the UMSOM scientists then searched a database of FDA-approved drugs to find those that are known to produce opposite patterns of those caused by the noise. (newswise.com)
  • EHRs provide real-world treatment and outcome patterns, while complementary biomolecular data, including disease-specific gene expression and drug-protein interactions, provide mechanistic understanding. (sutterhealth.org)
  • An alternative approach is gene coexpression analysis, which detects groups of genes with similar expression patterns across unrelated sets of transcriptomic data from the same organism. (mdpi.com)
  • Alternatively, gene expression patterns can be resolved using laser capture microdissection (LCM), which samples tissues on a grid. (scienceboard.net)
  • And next-generation sequencing (NGS) libraries can be used to quantify spatial gene expression patterns that delineate between cell populations. (scienceboard.net)
  • 3D reconstructions of gene expression patterns are done using an iterative proportional fitting (IFP) algorithm. (scienceboard.net)
  • The Tomographer framework was benchmarked for the ability to reconstruct transcriptome-wide spatial expression patterns against the Allen Adult Mouse Brain in situ hybridization atlas. (scienceboard.net)
  • By comparing gene expression patterns during embryogenesis or tissue differentiation, we unravel the molecular events that shape organisms, advancing our understanding of developmental biology. (pharmiweb.com)
  • Characterisation of gene expression patterns in bladder cancer allows the identification of pathways involved in its pathogenesis, and may stimulate the development of novel therapies targeting these pathways. (urotoday.com)
  • Some cause the expression of traits to deviate from the patterns predicted by Mendelian inheritance. (msdmanuals.com)
  • To analyze microarray datasets (34760 variables) and obtain functional implications of differential expression patterns, we used Linear Models for Microarray Data (limma) followed by Gene Set Enrichment Analysis (GSEA) using ClusterProfiler. (lu.se)
  • Research in gene expression is exploding right now and is examining both the impact of environmental factors and the promise of epigenetic therapies. (marksdailyapple.com)
  • Similarly, the neural circuits 'translate' the external stimulus, which is 'senseless' to genes, into a specific epigenetic information for their activation/inactivation. (cyclingforums.com)
  • New technologies such as in situ hybridization can be used to map the expression of multiple genes on the same tissue sample and have accelerated the generation of new atlases. (scienceboard.net)
  • In situ hybridization allows for a target gene to be tagged ("hybridized") with a fluorescent marker within sections of a tissue ("in situ") and visualized under a specialized microscope. (scienceboard.net)
  • Spatial transcriptomics provides quantitative gene expression data and visualization of the distribution of mRNAs within tissue sections and enables novel types of bioinformatics analyses, valuable in research and diagnostics. (lu.se)
  • With RNA-seq , we can capture and quantify the abundance of transcripts, revealing the dynamic landscape of gene expression within a cell or tissue. (pharmiweb.com)
  • With the advent of microarrays allowing the expression levels of increasing numbers of genes to be quantified, gene expression profiling, microarray analysis and transcriptomics have become frequently used terms associated with sampling RNA from multiple different tissue types. (videolectures.net)
  • Cell-free gene expression systems have been valuable tools for understanding how transcription/translation can be regulated in living cells. (nature.com)
  • PAR-2 and ERGIC-53 genes in HaCaT cells were up-regulated by 3.5 mW/cm2 millimeter wave exposure for 4 times. (nih.gov)
  • Gene expression in groups of ear cells after noise damage. (newswise.com)
  • After making their study known to other researchers in their field, the team heard from scientists at Decibel Therapeutics (led by Joe Burns, PhD) and the Karolinska Institute (led by Barbara Canlon, PhD), who had the gene expression data from the inner ear's neurons, side wall and immune cells from before and after noise damage. (newswise.com)
  • In another example, the researchers found that only one out of the four types of immune cells detected showed major differences in gene expression. (newswise.com)
  • The information stored in genes must be used to make products, such as enzymes, that cells need to perform different functions. (visionlearning.com)
  • De Iaco also found that when DUX4 is forcibly produced in muscle cells, it turns on a whole set of genes that are expressed during zygotic genome activation. (news-medical.net)
  • Next, they looked at mouse embryonic stem cells, which contain the mouse version of the DUX4 gene (called simply DUX). (news-medical.net)
  • When in culture, a small fraction of these cells exhibit at any given time the gene expression pattern of 2-cell stage embryos, before cycling back to the features of more advanced embryonic cells. (news-medical.net)
  • After many years of concerted effort to systematically analyze the expression of genes in all human cells and tissues, RIKEN and the FANTOM consortium publish the findings today in two landmark Nature reports, and 16 related articles in ten other scholarly journals (ref.1,2,3). (riken.jp)
  • Using a highly sensitive technique called Cap Analysis of Gene Expression (CAGE), developed at RIKEN (ref. 4, 5), the researchers monitored the activity of promoters and enhancers across over 180 human primary cells. (riken.jp)
  • The difference between all these cells is what parts of the genome they use - for instance, brain cells use different genes than liver cells, and therefore they work very differently. (riken.jp)
  • Unlike other large-scale genomics projects, FANTOM5 focused on identifying gene expression on normal primary cells rather than cell lines derived from cancers. (riken.jp)
  • Spatially resolved molecular atlases help scientists understand where different types of cells are located in the body and map their gene expression in specific locations in tissues and organs. (scienceboard.net)
  • Light entering the eye causes genes to be expressed in specific cells. (cyclingforums.com)
  • Synaptic transmitter binding to receptors on melatonin secreting cells in the pineal activate genes in the cell nucleus, but that involves a whole series of intermediate steps. (cyclingforums.com)
  • Now researchers from North Carolina State University have found a way to use naturally occurring hydrogen peroxide inside cells to switch on gene expression. (phys.org)
  • They designed a molecule that was sensitive to hydrogen peroxide and enabled expression of the firefly luciferase gene in live mammalian cells. (phys.org)
  • The exciting aspects of this synthetic gene switch are that you can use it not only as a way to detect the presence of hydrogen peroxide within cells, in this case, by making cells containing hydrogen peroxide glow, but also to express any gene of interest in response to the presence of hydrogen peroxide," Deiters says. (phys.org)
  • Hydrogen Peroxide Induced Activation of Gene Expression in Mammalian Cells using Boronate Estrone Derivatives" Jeane M. Govan, Andrew L. McIver, Chad Riggsbee and Alexander Deiters, North Carolina State University, Angewandte Chemie , 2012. (phys.org)
  • When studying gene expression, researchers often compare gene expression profiles between different biological samples or experimental conditions, such as healthy and diseased tissues, treated and untreated cells, or different developmental stages. (pharmiweb.com)
  • The locus of the psl polysacchide genes encode the cells to adhere to a surface and thus maintaining the biofilm structure. (ukessays.com)
  • Gene expression must be appropriately maintained to regulate development, differentiation, and proliferation of cells. (intechopen.com)
  • We used the paradigmatic GATA-PU.1 axis to explore, at the systems level, dynamic relationships between transcription factor (TF) binding and global gene expression programs as multipotent cells differentiate. (lu.se)
  • In addition, we enable manipulation of the cellular gene expression program by providing different genome-wide collections of ORFs. (epfl.ch)
  • Reverse-transcription polymerase chain reaction (RT-PCR) was performed to confirm the differential expression of genes obtained from Genechip analysis. (nih.gov)
  • Genomic imprinting is the differential expression of genetic material depending on whether it has been inherited from the father or mother. (msdmanuals.com)
  • In contrast, culturing on Laminin-421 induced the expression of β-cell genes, including Ins1 , MafA , and Glut2 , and significantly improved glucose-stimulated insulin secretion. (nature.com)
  • We identified 24 common genes that were significantly altered in both PMNs and PBMCs, including genes involved in IFN signaling that were downregulated after treatment in both cell populations, and IL1R1 that was upregulated. (frontiersin.org)
  • Differential Gene Expression analysis is a fundamental tool for identifying genes whose expression levels significantly differ between experimental conditions or biological samples. (pharmiweb.com)
  • We identified 66 genes significantly associated with total highest lifetime CAPS score (FDR-corrected p (cdc.gov)
  • In particular, we identified 82 genes significantly associated with lifetime anxious arousal symptoms. (cdc.gov)
  • Several genes significantly associated with multiple PTSD symptom dimensions and total lifetime CAPS score (SERPINA1, RPS6KA1, and STAT3) have been previously associated with PTSD. (cdc.gov)
  • Gene expression" refers to the process by which a DNA sequence (i.e. a gene) is used to make a specific RNA or protein, while "epigenetics" refers to the ways phenotypes are impacted by factors other than genetic sequence (e.g. gene repression by chromatin packing). (umassmed.edu)
  • How do parental life experiences impact offspring phenotypes without affecting their gene sequences? (umassmed.edu)
  • In addition to environmental factors, phenotypes can also respond to genetic perturbations in a plastic or homeostatic manner, which characterizes the potential of an organism to express phenotypes when genes mutate. (nature.com)
  • Future studies should be clear about methods used to analyze PTSD status, as phenotypes based on PTSD symptom dimensions may yield different gene sets than combined CAPS score analysis. (cdc.gov)
  • Amy Radunz, a nutritionist from the department of animal sciences at the University of Wisconsin in River Falls, has partnered with molecular biologist Hasan Khatib, an associate professor at University of Wisconsin-Madison, to ask how differences in maternal diet during pregnancy induce gene expression and DNA methylation changes in fetal tissues in sheep. (genengnews.com)
  • The early findings suggest space travel boosts methylation, the process of switching genes on and off. (upi.com)
  • Formation of this biomolecular condensate is strongly enhanced by a DNA modification called CpG methylation, a change that influences gene expression,' said co-corresponding author Dr. Kevin MacKenzie, associate professor of pathology and immunology and of pharmacology and chemical biology at Baylor. (news-medical.net)
  • With this study, we've seen thousands and thousands of genes change how they are turned on and turned off. (upi.com)
  • By analyzing the expression levels of thousands of genes simultaneously, researchers can identify genes that are upregulated (higher expression) or downregulated (lower expression) in one condition compared to another. (pharmiweb.com)
  • RNA polymerase II (Pol II) transcribes all protein-coding genes but also some non-coding RNAs (e.g., snRNAs, snoRNAs or long non-coding RNAs). (wikipedia.org)
  • Transcriptomics has also expanded from sampling protein coding genes to non-coding transcripts such as miRNAs, which have the potential not only to act as more stable biomarkers, but also to regulate translation, and in themselves be used a therapeutic agents against specific targets. (videolectures.net)
  • These findings will help in the identification of genes involved in disease and the development of personalized and regenerative medicine. (riken.jp)
  • DGE analysis facilitates the identification of genes responsive to specific drug treatments. (pharmiweb.com)
  • It is not the external stimulus per se , but the chemical output (=information) generated by processing of the stimulusin the neural circuit that via the respective signal transduction pathways reaches the genes responsible for the synthesis of melatonin, which is intelligible to (i.e. contains informatio for) activating those genes. (cyclingforums.com)
  • By subjecting DEGs to gene ontology enrichment analysis, pathway analysis, or functional annotation, we gain insights into the biological processes, molecular functions, and pathways associated with the observed gene expression changes. (pharmiweb.com)
  • In AAV groups, from the combined limma with GSEA using ClusterProfiler, we found both shared and unique gene sets and pathways for mice with wtHTT overexpression compared to mice with mHTT overexpression. (lu.se)
  • Multiple biosynthetic pathways such as sterol synthesis were among the top shared processes, where both unique and shared genes constituted leading-edge subsets. (lu.se)
  • Here we report the effect of the length of linearized reporter DNAs encoding the firefly luciferase gene so as to exclude the influence of supercoiling. (nature.com)
  • Researchers recently discovered a novel mechanism that works over an extensive genomic distance and controls the expression of human growth hormone (hGH) in the pituitary gland. (sciencedaily.com)
  • Researchers at the University of Pennsylvania School of Medicine recently discovered a novel mechanism that works over an extensive genomic distance and controls the expression of human growth hormone (hGH) in the pituitary gland. (sciencedaily.com)
  • While mutations to the hGH gene often lead to abnormal growth in children and adults, these mutations have provided researchers with key clues regarding the genomic areas that appear to control expression of the hGH gene. (sciencedaily.com)
  • At the genomic level, a 14,000 base-pair separation is equal to the size of 10 growth hormone genes lined end to end. (sciencedaily.com)
  • This alignment process enables the determination of the origin of each read, allowing us to associate it with specific genes or genomic regions. (pharmiweb.com)
  • Gene expression is summarized in the central dogma of molecular biology first formulated by Francis Crick in 1958, further developed in his 1970 article, and expanded by the subsequent discoveries of reverse transcription and RNA replication. (wikipedia.org)
  • Expressing a particular gene requires that the gene expression molecular machinery has access to that stretch of DNA. (news-medical.net)
  • The first 2 pairs were also enriched among pairs discovered using gene expression data and are supported by molecular interactions in drug-protein networks and preclinical and epidemiologic evidence. (sutterhealth.org)
  • The purpose of this study was to develop a robust, standardized method for comparative expression profiling and molecular characterization of Leishmania donovani clinical isolates. (cambridge.org)
  • Intracellular amastigote protocols need further refinement, but we describe a first generation tool for high-throughput comparative molecular characterization of patients' isolates, based on the changing expression profiles of promastigotes during in vitro differentiation. (cambridge.org)
  • There will be involved gene engineering and molecular biology methods such as PCR, SDS-PAGE, qPCR, western blot, DNA-protein interaction assays, flow cytometry etc. (lu.se)
  • Gene expression quantification of the sections is implemented using spatial transcriptomics by reoriented projections and sequencing (STRP-seq), a method that combines the sampling strategy presented above with a customized, low-input RNA-seq protocol based on single-cell tagged reverse transcription sequencing (STRT-seq) chemistry. (scienceboard.net)
  • And we can influence gene expression to a far greater degree than anyone ever thought possible. (marksdailyapple.com)
  • Diet, exercise, exposure to toxic chemicals (or fresh air), medicines, even the thoughts you think (which generate actual chemical signals) all influence gene expression - positively and/or negatively, depending on the choice. (marksdailyapple.com)
  • The study, published in the journal Nature Communications, reveals that the binding of KLF4 can cause DNA to condense into a separate liquid phase in a process called biomolecular condensation, which recruits other factors that influence gene expression. (news-medical.net)
  • Then, they compared 923 reconstructed genes to the in situ hybridization data from the mouse brain atlas using Pearson's correlation coefficient and found that the Tomographer workflow was more than twice as accurate as IPF. (scienceboard.net)
  • Once the reads are mapped, we quantify the expression level of each gene. (pharmiweb.com)
  • In this research project we try to quantify the gene expression of the above genes pilB, pslA, lecA, ureB using rpoD as the house keeping gene. (ukessays.com)
  • It is expected that the enhancement of gene expression efficiency with a shrunken DNA conformation would also be a rather general mechanism in living cellular environments. (nature.com)
  • Small and Large Ribosomal Subunit Deficiencies Lead to Distinct Gene Expression Signatures that Reflect Cellular Growth Rate. (broadinstitute.org)
  • With RNA-sequencing now becoming more affordable and with the advantages of identifying novel transcripts and detecting a greater range of expression levels, the future for gene expression profiling includes focusing on transcripts in specific cellular compartments, being an integral part of clinical trials and perhaps even a standard part of diagnostic procedures and therapeutic decisions. (videolectures.net)
  • Scientists armed with whole genome sequence data can now ask questions about genome structure and organization that impact gene expression. (genengnews.com)
  • The cell reads the sequence of the gene in groups of three bases. (lupusresearch.org)
  • Our aim was to clone and sequence the cDNA of the BB diabetes prone (DP) and diabetes resistant (DR) alleles of all seven Gimap genes in the congenic DR. lyp rat line with 2 Mb of BB DP DNA introgressed onto the DR genetic background. (hindawi.com)
  • W. B. Wang, S. Y. Zhu, L. Q. Wang, F. Yu, Shen W. D. (2005) Cloning and sequence analysis of the Antheraea pernyi nucleo-polyhedrovirus gp64 gene. (scirp.org)
  • E. A. van Strien, D. Zuidema, R.W. Goldbach, Vlak, J. M. (1992) Nucleotide sequence and transcriptional analysis of the polyhedrin gene of Spodoptera exigua nuclear polyhedrosis vi-rus. (scirp.org)
  • The account expands to include a brief discussion of other regulatory mechanisms that govern the expression of lambda's genome. (the-scientist.com)
  • The results of these studies have been used to investigate disease mechanisms, monitor progression of disease and identify specific genes or gene signatures which could be used as diagnostic or prognostic biomarkers. (videolectures.net)
  • Gene expression profiling has been a key methodology used in Sheffield and we initially used it to investigate disease mechanisms associated with mutant SOD1. (videolectures.net)
  • Although the majority of expression quantitative trait loci (eQTLs) for the gene expression traits in the two environments are shared and have similar effects, analysis of the environment-specific eQTLs reveals enrichment of binding sites for two transcription factors. (nature.com)
  • Lastly, we determined whether synergistic drug pairs found in the EHRs were enriched among synergistic drug pairs from gene-expression data using a method similar to gene set enrichment analysis. (sutterhealth.org)
  • Gene Set Enrichment Analysis (GSEA) with leading-edge analysis showed that suppressed sterol- and cholesterol metabolism were shared between hypothalamic wtHTT and mHTT overexpression. (lu.se)
  • In this study, we investigated whether hypothalamic HTT expression causes transcriptional changes. (lu.se)
  • The study also looked at the impact of paternally expressed genes: IGF2, PEG1, PEG3, DLK1, and DIO3 for transcriptomic and epigenomic alterations of the imprinted genes in fetal tissues. (genengnews.com)
  • The papers published in Nature describe maps of promoters and enhancers - short regions of DNA that influence the activity of genes - encoded in the human genome, and their activity across the vast wealth of human cell types and tissues of the human body. (riken.jp)
  • April 21, 2021 -- A new framework called Tomographer, which uses sequencing data from tissues cut into thin strips in a way that allows them to be reconstructed, has been developed to spatially resolve gene expression data without the need for a microscope. (scienceboard.net)
  • Using a lentiviral vector containing either H-Ras oncogene and a hairpin targeting p53 or both tumor suppressor genes, NF-1 and p53, Dr. Friedmann-Morvinski can induce the formation of a glioma tumor in mice injected in the brain. (genengnews.com)
  • To test whether the transcription of the locus control region and CD79b played a significant role in hGH expression in transgenic mice, Ho then inserted a special piece of DNA into the locus control region. (sciencedaily.com)
  • BACHD mice with ubiquitous expression of full-length mutant HTT (mHTT) and wild-type mice with targeted hypothalamic overexpression of either wild-type HTT (wtHTT) or mHTT fragments. (lu.se)
  • This study involves a comprehensive, multi-modal, and integrative assessment of biomarkers implicated in the pathophysiology of PTSD, including measuring differences in whole-blood gene expression and other blood biomarkers of key neurobiological systems, an approach critical to informing risk and resilience prediction algorithms for PTSD, and to develop novel psychopharmacologic approaches for the treatment of this disabling condition in disaster responders and other trauma survivors. (cdc.gov)
  • Analysis of gene expression from these individuals can help in identifying biomarkers of PTSD. (cdc.gov)
  • For example, steroid and thyroid hormones directly bind to nuclear receptors, which induce expression of specific genes. (intechopen.com)
  • RNA polymerase III transcribes 5S rRNA, transfer RNA (tRNA) genes, and some small non-coding RNAs (e.g., 7SK). (wikipedia.org)
  • This mechanism involves a newly discovered set of "non-coding RNAs" expressed in the vicinity of the hGH gene. (sciencedaily.com)
  • By examining the relationship between these non-coding RNAs and the hGH gene, researchers hope to understand how these remote regions impact hGH gene expression and dysfunction. (sciencedaily.com)
  • When you change to a diet low in sugars and rich in healthy fats, those or other genes are directed to reduce inflammatory expression, down-regulate insulin-producing metabolic machinery, up-regulate insulin receptors and rebuild cell membranes to reflect the presence of better building materials (omega 3 fatty acids, etc. (marksdailyapple.com)
  • FANTOM, a large international consortium led by RIKEN releases today the first comprehensive map of gene activity across the human body, and provides the first holistic view of the complex networks that regulate gene expression across the wide variety of cell types that make up a human being. (riken.jp)
  • A key discovery on the way was that by employing CAGE, the technology used to find active genes, the team could identify the additional DNA regions that regulate the activity of genes in every cell type, called enhancers. (riken.jp)
  • Genetic variance in expression increases at 18 °C relative to 25 °C for most genes that have a change in genetic variance. (nature.com)
  • A Genetic Switch: Gene Control and Phage A. Mark Ptashne. (the-scientist.com)
  • Keeping the boron out of the ER: A genetic switch was engineered that activates gene expression in the presence of H2O2. (phys.org)
  • In other words, expression of specific genes might have relevance to pathogenesis of diseases. (intechopen.com)
  • Recent global analyses of gene transcripts revealed that specific transcription factors (TFs) and their networking systems physiologically correspond to the onset of human diseases, including cancer. (intechopen.com)
  • Our more granular analyses of PTSD symptom dimensions identified additional genes that did not reach statistical significance in our analyses with total CAPS scores. (cdc.gov)
  • Positional cloning of lymphopenia ( lyp ) in the BB rat revealed a frameshift mutation in Gimap5 , a member of at least seven related GTPase Immune Associated Protein genes located on rat chromosome 4q24. (hindawi.com)
  • Gimap5 is a member of at least seven related GTPase Immune Associated Protein ( Gimap ) genes located within 150 Kilobases (Kb) on rat chromosome (RNO) 4 [ 2 , 3 ]. (hindawi.com)
  • In the current study, we focused on master transcription factor KLF4, which is known to selectively mediate gene expression and reprogramming that determines cell fate. (news-medical.net)
  • Dr. Hertzano says it was so important that they looked at a cell specific level, rather than looking at the entire ear because they found that most of the gene expression changes were specific to only one or two cell types. (newswise.com)
  • But when the EPFL researchers deleted the DUX gene, this process stopped, the appearance of the 2-cell stage-like subpopulation was suppressed. (news-medical.net)
  • In FANTOM5, we have for the first time systematically investigated exactly what genes are used in virtually all cell types across the human body, and the regions which determine where the genes are read from the genome," explains Dr. Alistair Forrest, scientific coordinator of FANTOM5. (riken.jp)
  • At this time, there is a paucity of information as to the expression of the Gimap genes in specific cell types. (hindawi.com)
  • A very similar set of genes is induced in unlesioned animals by administration of the psychostimulant cocaine or the antipsychotic eticlopride, although in distinct striatal cell populations. (jneurosci.org)
  • When hydrogen peroxide was present, the luciferase gene was expressed, causing the cell to glow. (phys.org)
  • While the CD79b gene normally codes for a protein in blood lymphocytes, researchers discovered that CD79b appears to play a very different role in the pituitary gland. (sciencedaily.com)
  • A team led by researchers at Baylor College of Medicine has discovered a mechanism by which transcription factor KLF4 can help to organize chromatin, thus influencing gene expression. (news-medical.net)
  • The team added their newest data on noise-induced hearing loss to gEAR - Gene Expression Analysis Resource - a tool developed by her laboratory that allows researchers not trained in informatics to browse gene expression data ( published earlier this summer ). (newswise.com)
  • The way that genes translate into physical traits has to do with the particular enzyme that each type of gene makes, a discovery that was made by two scientists by way of the mutant bread mold they created, winning them the Nobel Prize in 1958. (visionlearning.com)
  • It wasn't until 1941 that George Beadle and Edward Tatum figured out the mechanism by which genes are translated into physical traits . (visionlearning.com)
  • In contrast, the ubiquitous expression of full-length mHTT in the BACHD hypothalamus moderately affects the transcriptomic profile. (lu.se)
  • When KLF4 interacts with specific chromatin regions, it forms a condensate - a separate liquid phase - that preferentially recruits other molecules that help open the chromatin and mediate gene transcription. (news-medical.net)
  • Transcription factors such as KLF4 are involved in reorganizing the chromatin to provide access and facilitate gene transcription, but it is not clear how this is accomplished. (news-medical.net)
  • The most common approach in transcriptomics (RNA-seq and microarrays) is differential gene expression analysis (DGEA). (mdpi.com)
  • A classic example is the heat shock protein system, particularly Hsp90 , whose expression is environmentally plastic and increases under thermal stress, but buffers phenotypic changes induced by mutations to maintain homeostasis 2 , a process termed canalization 3 . (nature.com)
  • This analysis enables us to pinpoint genes that play pivotal roles in phenotypic variation, disease development, or response to treatments. (pharmiweb.com)
  • The expression level from luc 25.7k is 1000-times higher than that with luc 1.7k, indicating that the protein yield per target gene is increased by 1000-times of magnitude for a longer template, luc 25.7k (Fig. 1 d). (nature.com)
  • Sex-limited inheritance, perhaps more correctly called sex-influenced inheritance, refers to special cases in which sex hormones and other physiologic differences between males and females alter the expressivity and penetrance of a gene. (msdmanuals.com)
  • The process of gene expression is used by all known life-eukaryotes (including multicellular organisms), prokaryotes (bacteria and archaea), and utilized by viruses-to generate the macromolecular machinery for life. (wikipedia.org)
  • The FANTOM project (for Functional Annotation of the Mammalian genome) is a RIKEN initiative launched in 2000 to build a complete library of human genes using the capabilities offered by new, state-of-the-art cDNA technologies. (riken.jp)
  • Work in our department has developed a rapid evolution computational protocol whereby the fitness of thousands of mutations of any given gene can be assessed simultaneously. (umassmed.edu)
  • AFM observation of the DNA conformation indicates that longer DNA takes shrunken conformation with a higher segment density in the reaction mixture for gene expression, in contrast to the stiff conformation of shorter DNA. (nature.com)
  • In contrast to some previously described early genes, most of the novel genes are not induced in cortex by apomorphine, indicating specificity of induction. (jneurosci.org)
  • Although these factors have been studied extensively, little is known about how the length of the DNA template influences gene expression. (nature.com)
  • In line with this lead, the scientists could show that the DUX4 protein binds to the regulatory region of genes that are induced during zygotic genome activation, stimulating their expression. (news-medical.net)
  • The final piece of evidence came when the EPFL scientists removed the DUX gene from fertilized mouse oocytes using CRISPR/Cas9 genome editing. (news-medical.net)
  • In the current study, scientists at the Swiss Federal Institute of Technology Lausanne (EPFL) School of Life Sciences described how they have created a computational algorithm called Tomographer that can transform gene-sequencing data into spatially resolved data such as images, without using a microscope. (scienceboard.net)
  • Nucleus-specific expression in the multinuclear mushroom-forming fungus reveals different nuclear regulatory programs. (broadinstitute.org)
  • and (4) how dynamic modeling of DNA-binding and gene expression data can be used to infer regulatory logic of TF circuitry. (lu.se)
  • CONCLUSIONS: This is a proof-of-concept study demonstrating that a combination of complementary data sources, such as EHRs and gene expression, can corroborate discoveries and provide mechanistic insight into drug synergism for repurposing. (sutterhealth.org)
  • Our data substantiates the Gimap5 frameshift mutation as the primary defect with only limited contributions to lymphopenia from the remaining Gimap genes. (hindawi.com)
  • Because TNF-alpha expression is upregulated in hepatic inflammation, the data suggest TNF-alpha-mediated increases in tTG expression may play an important role in the process of hepatic fibrogenesis. (nih.gov)
  • Using a genetically modified mouse model, Nancy E. Cooke, MD, Stephen A. Liebhaber, MD, Professors of Genetics and Medicine, and colleagues, demonstrated a critical role of two non-coding regions on the activation of the hGH gene. (sciencedaily.com)
  • By carefully analyzing the 14,000 base pairs separating the hGH gene and its locus control region, co-authors Yugong Ho, PhD, an Instructor of Genetics at Penn and a Cooke/Liebhaber lab member, and Felice Elefant, PhD, Assistant Professor at Drexel University and former member of the Cooke/Liebhaber lab, found that the locus control region was copied into RNA, and discovered a gene called CD79b within this region. (sciencedaily.com)
  • Overview of Genetics A gene, the basic unit of heredity, is a segment of DNA containing all the information necessary to synthesize a polypeptide (protein) or a functional RNA molecule. (msdmanuals.com)
  • We expected the subset of neurons typically sensitive to noise and aging, to have "bad" changes in genes, so that we could counter them with drugs, but there was no such thing," said Dr. Hertzano. (newswise.com)
  • On the contrary, we found that the subset of neurons that are resistant to noise trauma, turn on a program that protects them while the very sensitive neurons had little change in gene expression. (newswise.com)
  • The specific expression and distribution of the different BM components in the islets were yet to be fully characterized. (nature.com)
  • The external stimulus per se is neutral to any known pathway in metazoans, it can't activate any specific gene. (cyclingforums.com)
  • this is particularly a delicate task for intracellular amastigotes and for specific 'sensory' genes. (cambridge.org)
  • We chose to use expression-curves rather than single, specific, time-point measurements to capture this biological variation. (cambridge.org)
  • Previous work in the laboratories of Cooke and Liebhaber found that the hGH gene is controlled by a non-coding DNA region, or locus control region. (sciencedaily.com)
  • The effects of the locus control region on human growth hormone expression is as if you turn a key in the lock of a house at one end of your street and find that this action opens the lock and door of a house a block away," notes Liebhaber. (sciencedaily.com)
  • RNAseq of the dorsolateral prefrontal cortex and the nucleus accumbens, two brain areas strongly associated with addiction pathophysiology, showed that certain genes were expressed differently in people with OUD compared to controls. (eurekalert.org)
  • We combined global ChIP-seq of GATA1, GATA2, and PU.1 with expression profiling during differentiation to erythroid and neutrophil line- ages. (lu.se)