AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation Science
Chromatin Assembly Factor-1ChromatinChromatin Assembly and DisassemblyHistonesNucleosomesChromosomal Proteins, Non-HistoneRetinoblastoma-Binding Protein 4DNA-Binding ProteinsMolecular ChaperonesRetinoblastoma-Binding Protein 7DNA ReplicationSaccharomyces cerevisiae ProteinsHeterochromatinSaccharomyces cerevisiaeNuclear ProteinsProliferating Cell Nuclear AntigenHistone AcetyltransferasesCell Cycle ProteinsNucleosome Assembly Protein 1Protein BindingChromatin ImmunoprecipitationCell NucleusGene SilencingHeLa CellsHistone ChaperonesMolecular Sequence DataMutationS PhaseAcetylationDNA DamageAmino Acid SequenceTranscription, GeneticMicrococcal NucleaseFungal ProteinsApc5 Subunit, Anaphase-Promoting Complex-CyclosomeNucleoplasminsDNATranscription FactorsCentromerePromoter Regions, GeneticDNA, SuperhelicalBase SequenceGene Expression Regulation, FungalProtein Structure, TertiaryRibosome Subunits, Large, EukaryoticRepressor ProteinsCell LineBinding SitesProtein SubunitsMitosisAcetyltransferasesEpigenesis, GeneticCell ExtractsDrosophila ProteinsDeoxyribonuclease IHistone DeacetylasesVirus AssemblyDrosophilaLysineSex ChromatinAdenosine TriphosphatasesModels, BiologicalGene Expression RegulationXenopus laevisTwo-Hybrid System TechniquesChromosomesDNA RepairCell CycleXenopusSequence Homology, Amino AcidMethylationMacromolecular SubstancesYeastsModels, MolecularPlasmidsGene DeletionOocytesElectron Transport Complex IRecombinant ProteinsMultiprotein ComplexesRecombinant Fusion ProteinsCarrier ProteinsTranscriptional ActivationMitochondrial DiseasesDrosophila melanogasterChickensRNA Polymerase IIPhenotypeModels, GeneticHigh Mobility Group Proteins
SubunitHistonesProteinsProteinNucleosomesUnit of chromatinGenesDisassemblyOrganizationASF1ReplicationEpigeneticsNuclearComplexesDynamicsChaperonesRepressionRegulatesCell differentiationCompactionDrosophilaVivoFunctionally distinctNucleusGeneHIRAEukaryoticVitroReorganizationRole of histoneComplexGenome stabilityP1502023StructureBindsBiologicalHistone octamerREMODELINGModificationsConformationLocalizationStepwiseSMRTAcetylationP105Repressive
Subunit10
  • The human subunit (p150) interacts with PCNA, which acts as a sliding clamp, to help the CAF-1 complex interact with the DNA replication fork. (wikipedia.org)
  • A mutation like this in either subunit would result in loss of function for the CAF-1 complex as a whole. (wikipedia.org)
  • The 48 kDa subunit, RETINOBLASTOMA-BINDING PROTEIN 4, is also a component of several other protein complexes involved in chromatin remodeling. (lookformedical.com)
  • Although initially discovered as a retinoblastoma binding protein it has an affinity for core HISTONES and is a subunit of chromatin assembly factor-1 and polycomb repressive complex 2. (lookformedical.com)
  • Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. (chromatine.com)
  • Here, we report on a loss-of-function mutant allele of the FASCIATA1 (FAS1) subunit of the chromatin assembly factor 1 (CAF-1) complex that suppresses the phenotype of WEE1- or ATR-deficient Arabidopsis (Arabidopsis thaliana) plants. (muni.cz)
  • Upregulation of Phosphatase 1 Nuclear-Targeting Subunit (PNUTS) Is an Independent Predictor of Poor Prognosis in Prostate Cancer. (nih.gov)
  • Phosphatase 1 Nuclear Targeting Subunit (PNUTS) Regulates Aurora Kinases and Mitotic Progression. (nih.gov)
  • Biophysical Analysis of the N-Terminal Domain from the Human Protein Phosphatase 1 Nuclear Targeting Subunit PNUTS Suggests an Extended Transcription Factor TFIIS-Like Fold. (nih.gov)
  • We report the crystal structure of an ASF1a-HIRA heterodimer and a biochemical dissection of ASF1a's mutually exclusive interactions with HIRA and the p60 subunit of CAF-1. (omicsdi.org)
Histones21
  • The CAF-1 complex adds the histones to the DNA ahead of the replication fork. (wikipedia.org)
  • ASF1 is required to load histones on the HIRA complex in preparation of paternal chromatin assembly at fertilization. (ens-lyon.fr)
  • The main proteins found in chromatin are called histones. (hstalks.com)
  • The additional aspect that histones can bring in the chromatin organization is a source of information. (hstalks.com)
  • First, you have the DNA in the form of a helix, which is wrapped around the core histones to form the nucleosome-the sort of tennis ball that was represented before- which then folds up further to give chromatin fibers, which further fold up and then organize in the nucleus to form different domains. (hstalks.com)
  • Maintaining a proper supply of soluble histones throughout the cell cycle is important to ensure chromatin and genome stability. (biomedcentral.com)
  • Following their synthesis, histones undergo a series of maturation steps to prepare them for deposition onto chromatin. (biomedcentral.com)
  • We find that depletion of JMJD1B increases the protein levels of the histone chaperone tNASP leading to an accumulation of newly synthesized histones H3 and H4 at early steps of the histone maturation cascade, which perturbs chromatin assembly. (biomedcentral.com)
  • The major event of MBT was the activation of a large group of histones and other genes that modify chromatin structure preceding massive gene expression changes. (biomedcentral.com)
  • We find that CAF-1 deficient cells as well as cells affected in newly-synthesized H3-H4 histones deposition during DNA replication exhibit a severe negative growth with ctf4 ∆ mutant. (cell-stress.com)
  • Collectively, our results suggest that the deposition by CAF-1 of newly-synthesized H3-H4 histones during DNA replication creates a chromatin environment that favors sister chromatid cohesion and maintains genome integrity. (cell-stress.com)
  • Here, we identify mutants of histones H3.1 and H3.3 that are unable to interact with human ASF1A and ASF1B isoforms but that are still competent to bind CAF-1 and HIRA, respectively. (omicsdi.org)
  • We show that these mutant histones are inefficiently deposited into chromatin in vivo. (omicsdi.org)
  • Consistent with this, biochemical analysis of wild-type and phospho-mimetic Asf1a shows that phosphorylation enhances binding to histones and the downstream chaperones CAF-1 and HIRA. (omicsdi.org)
  • We thus propose that TLK signalling promotes histone supply in S phase by targeting histone-free Asf1 and stimulating its ability to shuttle histones to sites of chromatin assembly. (omicsdi.org)
  • No, but they have similarly positively charged proteins, like IHF (Integration host factor) What are the four histones making up the core particle making up the histone octamer? (usadellab.org)
  • While canonical histones H3.1 and H3.2 are synthetized and loaded during DNA replication, the histone variant H3.3 is expressed and deposited into the chromatin throughout the cell cycle. (aging-us.com)
  • Among these regulatory processes, core and linker histones are subjected to a large pattern of posttranslational modifications (PTMs) that influence chromatin state and DNA accessibility [ 4 - 7 ]. (aging-us.com)
  • Along with PTMs, the partial or complete disassembly of nucleosomes allows the exchange and degradation of pre-existing histone proteins, with the incorporation of newly synthesised histones onto chromatin that can eventually result in the resetting of previous epigenetic marks. (aging-us.com)
  • Nucleosome assembly proteins (NAPs) are histone chaperones that are crucial for the shuttling and incorporation of histones into nucleosomes. (biomedcentral.com)
  • In addition to playing an important role in histone exchange during nuclear processes, histone chaperones function in nucleocytoplasmic shuttling of histones, in histone storage, in nucleosome assembly and they act as a link between chromatin remodeling factors and histones [ 2 , 3 ]. (biomedcentral.com)
Proteins14
  • A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. (lookformedical.com)
  • This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS. (lookformedical.com)
  • Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. (chromatine.com)
  • The conserved family of Transcription Intermediary Factors (TIF1) proteins consists of key transcriptional regulators that control transcription of target genes by modulating chromatin state. (elifesciences.org)
  • DNA in the nucleus is present in the form of chromatin, and that consists of a complex together with proteins. (hstalks.com)
  • SMRT and NCoR are homologous corepressor proteins that are recruited to many repressive transcription factors. (esrf.fr)
  • Hence the tetrameric nature of the TBL1 suggests that the protein may serve as a scaffold for a multivalent chromatin-targeted repression machine that contains multiple co-repressor proteins and histone deacetylase enzymes. (esrf.fr)
  • All living organisms package their DNA with chromatin proteins. (biologists.com)
  • A hallmark of events observed during segmentation was the induction of multiple transcription factors, including a large group of homeobox proteins in pace with decay of a large fraction of maternal transcripts. (biomedcentral.com)
  • These factors bind cooperatively to the IFN-beta enhancer and recruit coactivators and chromatin-remodeling proteins to the IFN-beta promoter. (rcsb.org)
  • We also discuss normal synthesis of tachykinins and their receptors, yet focus on their regulation by microRNA, messenger RNA stabilizing proteins, and transcription factors like Restrictive Element-1 Silencing Factor (REST). (brainimmune.com)
  • These dedicated proteins are generally known as histone chaperones and effectively control histone supply and chromatin dynamics [ 8 ]. (aging-us.com)
  • The human malaria parasite Plasmodium falciparum contains two nucleosome assembly proteins termed PfNapL and PfNapS. (biomedcentral.com)
  • Specific cloned DNA sequences can be assembled with proteins isolated from cellular extracts to provide details on the binding of each factor and its effect on the overall structure and activity of the complex. (psu.edu)
Protein26
  • Chromatin assembly factor-1 (CAF-1) is a protein complex - including Chaf1a (p150), Chaf1b (p60), and p48 subunits in humans, or Cac1, Cac2, and Cac3, respectively, in yeast- that assembles histone tetramers onto replicating DNA during the S phase of the cell cycle. (wikipedia.org)
  • A retinoblastoma-binding protein that is involved in CHROMATIN REMODELING, histone deacetylation, and repression of GENETIC TRANSCRIPTION. (lookformedical.com)
  • The repeating structural units of chromatin, each consisting of approximately 200 base pairs of DNA wound around a protein core. (lookformedical.com)
  • Chromatin immunoprecipitation sequencing in T helper 17 (T(H)17) cells reveals that IRF4 targets sequences enriched for activating protein 1 (AP-1)-IRF composite elements (AICEs) that are co-bound by BATF, an AP-1 factor required for T(H)17, B, and dendritic cell differentiation. (nih.gov)
  • We have shown that the invasion of DNA strands into a donor template region requires the action of the chromatin remodeling protein Rad54 that enables the recombination machinery to gain access to "closed" regions of DNA. (brandeis.edu)
  • The bromodomain protein Brd4 insulates chromatin from DNA damage signalling. (duke.edu)
  • The ORC-Cdc6 complex (product 1) assembles in step 1 around origin DNA and with the help of another replication initiator protein, Cdt1, it recruits the Mcm2-7 hexamer to the origin in step 2. (nature.com)
  • The conserved "core" of the SMRT/NCoR repression complexes (called repression domain 1 or RD1) recruits HDAC3, TBL1 (and/or its homologue TBLR1) as well as a protein called GPS2 (also known as AMF1). (esrf.fr)
  • Also, a reduction in hPaf1 level resulted in reduced MLL1 expression and a corresponding decrease in the level of CHD1 (Chromohelicase DNA-binding protein 1), an ATPase dependent chromatin remodeling enzyme that specifically binds to H3K4 di and trimethyl marks. (nebraska.edu)
  • Overall, our results suggest that hPaf1/PD2 in association with MLL1 regulates methylation of H3K4 residues, as well as interacts and regulates nuclear shuttling of chromatin remodeling protein CHD1, facilitating its function in pancreatic cancer cells. (nebraska.edu)
  • The transcription of protein-coding genes involves RNA polymerase II and a number of common factors (TFIIA, -B, -D, -E, -F and -H) that form functional preinitiation complexes (on promoters) via an ordered assembly pathway. (rockefeller.edu)
  • We therefore asked what role JMJD1B plays in newly synthesized histone maturation by exploiting the knockdown of JMJD1B by treating HeLa cells with siRNA against JMJD1B (siJMJD1B) and observed the reduction of both JMJD1B protein on cytosolic extracts (Fig. 1 b) and mRNA levels (Fig. 1 c) 72 h after the treatment. (biomedcentral.com)
  • This gene encodes a protein phosphatase 1 binding protein. (nih.gov)
  • The encoded protein plays a role in many cellular processes including cell cycle progression, DNA repair and apoptosis by regulating the activity of protein phosphatase 1. (nih.gov)
  • MYC dephosphorylation by the PP1/PNUTS phosphatase complex regulates chromatin binding and protein stability. (nih.gov)
  • The large ~280-kDa U5 snRNP protein PRPF8 is central to the dynamics of spliceosome assembly [ 11 ]. (biomedcentral.com)
  • Structural basis of BAM-mediated outer membrane β-barrel protein assembly. (ucsf.edu)
  • To test our hypothesis that histone H4R3 and H3R17 methylation regulates ferritin transcription, H4R3 and H3R17 protein arginine (R) methyltransferases 1 and 4 (PRMT1 and PRMT4) were investigated. (embl.de)
  • We describe the residue-specific methylation of E2F-1 by the asymmetric dimethylating protein arginine methyltransferase 1 (PRMT1) and symmetric dimethylating PRMT5 and relate the marks to different functional consequences of E2F-1 activity. (embl.de)
  • The Tudor domain protein p100-TSN reads the symmetric methylation mark, and binding of p100-TSN downregulates E2F-1 apoptotic activity. (embl.de)
  • The assembly of PRMT6 protein network allowed us to formulate functional hypotheses which led to the discovery of new molecular partners for the architectural transcription factor HMGA1a, a known substrate for PRMT6, and to provide evidences for a modulatory role of HMGA1a on the methyltransferase activity of PRMT6. (embl.de)
  • Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation. (embl.de)
  • Chromatin target of Prmt1 (Chtop) is a vertebrate-specific chromatin-bound protein that plays an important role in transcriptional regulation. (embl.de)
  • One high-resolution method of mapping DNA-protein contacts that can be used with both reconstituted and cellular assemblies is DNA footprinting. (psu.edu)
  • [ 1 ] His prediction was subsequently supported by the cloning of the retinoblastoma tumor suppressor gene ( RB1 ) and by functional studies of the retinoblastoma protein, Rb. (medscape.com)
  • The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)
Nucleosomes3
Unit of chromatin3
  • The elementary unit of chromatin is a nucleosome in which approximately 147 bp DNA is wrapped 1.67 turns around a histone octamer ( Fierz and Poirier, 2019 ). (molcells.org)
  • The nucleosome is the basic packaging unit of chromatin. (aging-us.com)
  • The repeating unit of chromatin is the nucleosome, formed from two histone H2A-H2B dimers and one histone H3-H4 tetramer around which 147 bp of DNA are wrapped [ 1 ]. (biomedcentral.com)
Genes9
  • They are recruited to cis-regulatory Ets-IRF composite elements by PU.1 or Spi-B. How these IRFs target genes in most T cells is enigmatic given the absence of specific Ets partners. (nih.gov)
  • To this end, our specific objectives are to determine the nature and mechanism of action of both the general transcription initiation factors that are commonly used by all genes and the gene- and cell type-specific factors that directly regulate target genes in response to various growth, developmental and viral stimuli. (rockefeller.edu)
  • Similarly, the transcription of small structural RNA genes (5S RNA, tRNA) by RNA polymerase III involves the assembly of common factors (TFIIIC and TFIIIB) into highly stable complexes via an ordered pathway. (rockefeller.edu)
  • Consistent with this idea, we confirm that CAF-1 synchronously binds two H3-H4 dimers derived from two different histone genes in vivo. (elsevierpure.com)
  • Furthermore, we describe genetic interactions between CAF-1 and essential genes involved in cohesin loading, cohesin stabilization, and cohesin component indicating that CAF-1 is crucial for viability when sister chromatid cohesion is affected. (cell-stress.com)
  • Antioxidant genes such as ferritin are transcriptionally activated in oxidative stress via the antioxidant responsive element (ARE), to which nuclear factor-E2-related factor 2 (Nrf2) binds and activates transcription. (embl.de)
  • Hox genes encode transcription factors that specify segmental identities along the Antero-Posterior body axis. (biorxiv.org)
  • In Drosophila , the BX-C cluster contains the three most posterior Hox genes, where their collinear activation incorporates progressive replacement of histone modifications, reorganization of 3D chromatin architecture and sequential activation of boundary elements and cis -regulatory regions. (biorxiv.org)
  • The collinear activation of the Hox genes and their cis -regulatory elements is thought to rely on the progressive opening of the chromatin within the BX-C along the A-P axis. (biorxiv.org)
Disassembly1
  • Histone chaperones have been implicated in nucleosome assembly and disassembly as well as histone modification. (omicsdi.org)
Organization5
  • CAF-1 is required for the spatial organization and epigenetic marking of heterochromatin domains in pluripotent embryonic cells, creating a cellular memory of somatic cell identity during cellular differentiation. (wikipedia.org)
  • Chromatin organization is dynamically altered for diverse DNA metabolic transactions such as replication, transcription, and repair. (molcells.org)
  • Trans -acting splicing factors, pre-mRNA secondary structure and chromatin organization are now known to directly affect alternative splicing decisions [ 6 ]. (biomedcentral.com)
  • To dissect functional hierarchies, we compared chromatin organization in larvae and in cell lines, with a focus on the Abd-B gene. (biorxiv.org)
  • This chromatin organization differentially instructed alternative Abd-B promoter use, thereby expanding the possibilities to regulate transcriptional output. (biorxiv.org)
ASF18
  • BACKGROUND: Anti-Silencing Factor 1 (ASF1) is a conserved H3-H4 histone chaperone involved in both Replication-Coupled and Replication-Independent (RI) nucleosomeassembly pathways. (ens-lyon.fr)
  • At DNA replication forks, ASF1 plays an important role in regulating the supply of H3.1/2 and H4 to the CAF-1 chromatin assembly complex. (ens-lyon.fr)
  • ASF1 also provides H3.3-H4 dimers to HIRA and DAXX chaperones for RI nucleosome assembly. (ens-lyon.fr)
  • In asf1 KD embryos, HIRA localizes to the male nucleus but is only capable of limited and insufficient chromatin assembly. (ens-lyon.fr)
  • This separation of tasks could optimize the rapid assembly of paternal chromatin within the gigantic volume of the egg cell.In contrast, ASF1 is surprisingly dispensable for the amplification of cleavage nuclei, although chromatin integrity is likely compromised in KD embryos. (ens-lyon.fr)
  • Following acetylation, newly synthesized H3-H4 is directly transferred from the histone chaperone anti-silencing factor 1 (Asf1) to chromatin assembly factor 1 (CAF-1), another histone chaperone that is critical for the deposition of H3-H4 onto replicating DNA. (elsevierpure.com)
  • In vivo study of the nucleosome assembly functions of ASF1 histone chaperones in human cells. (omicsdi.org)
  • ASF1 is a highly conserved histone H3/H4 chaperone that synergizes in vitro with two other histone chaperones, chromatin assembly factor 1 (CAF-1) and histone repression A factor (HIRA), in DNA synthesis-coupled and DNA synthesis-independent nucleosome assembly. (omicsdi.org)
Replication12
  • CAF-1 functions as a histone chaperone that mediates the first step in nucleosome formation by tetramerizing and depositing newly synthesized histone H3/H4 onto DNA rapidly behind replication forks. (wikipedia.org)
  • Several studies have shown that the interaction between CAF-1 and PCNA (proliferating cell nuclear antigen, which stabilizes CAF-1 at replication forks, is important for CAF-1's role in nucleosome assembly The three subunits work together to make the complex function. (wikipedia.org)
  • 1 Thecurrently available treatments use a combination of an HCV protease inhibitor withribavirin and PEGylated alpha interferon to disrupt virus replication, but the therapyis effective in only half of the people infected with HCV genotype 1 and even in thosepatients the efficacy is limited. (nature.com)
  • Together, the data illustrate a clear mechanism for CAF-1- associated H3-H4 chaperone activity in the context of de novo nucleosome (re)assembly following DNA replication. (elsevierpure.com)
  • Chromatin assembly and the establishment of sister chromatid cohesion are intimately connected to the progression of DNA replication forks. (cell-stress.com)
  • Here we examined the genetic interaction between the heterotrimeric chromatin assembly factor-1 (CAF-1), a central component of chromatin assembly during replication, and the core replisome component Ctf4. (cell-stress.com)
  • In the absence of CTF4 , CAF-1 is essential for viability in cells experiencing replication problems, in cells lacking functional S-phase checkpoint or functional spindle checkpoint, and in cells lacking DNA repair pathways involving homologous recombination. (cell-stress.com)
  • From a phosphoproteomic mass spectrometry screen, we identified and validated that ZL-2201 and PRKDC siRNA decreased Ser108 phosphorylation of MCM2, a key DNA replication factor. (bvsalud.org)
  • Our current research now focuses on two basic, interrelated questions: (1) How do mammalian cells decide where and when to initiate DNA replication? (nih.gov)
  • Human HIRA, ASF1a, ASF1b and CAF-1 are evolutionally conserved histone chaperones that form multiple functionally distinct chromatin-assembly complexes, with roles linked to diverse nuclear process, such as DNA replication and formation of heterochromatin in senescent cells. (omicsdi.org)
  • The dynamic nature of chromatin establishes the access to the genetic material and, as a consequence, influences a large number of biological processes, such as DNA replication, repair and transcription [ 1 , 2 ]. (aging-us.com)
  • Chromatin is highly dynamic, a characteristic that is vital in regulating nuclear processes such as transcription and replication which require access to DNA. (biomedcentral.com)
Epigenetics1
Nuclear10
  • Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro. (fishersci.com)
  • We investigated its cellular localization and found it in both cytosolic and nuclear extracts derived from HeLa cells (Fig. 1 a). (biomedcentral.com)
  • BAF nuclear assembly factor 1 [Sourc. (gsea-msigdb.org)
  • Previous studies have demonstrated that fractal characteristics of nuclear chromatin are of prognostic importance in neoplasias. (biomedcentral.com)
  • We have therefore investigated whether the fractal dimension of nuclear chromatin measured in routine histological preparations of malignant melanomas could be a prognostic factor for survival. (biomedcentral.com)
  • Nuclear area, form factor and fractal dimension of chromatin texture were obtained from digitalized images of hematoxylin-eosin stained tissue micro array sections. (biomedcentral.com)
  • Tumor thickness, Clark's level, mitotic rate, nuclear area and fractal dimension were significant risk factors in univariate Cox regressions. (biomedcentral.com)
  • In the multivariate Cox regression, stratified for the presence or absence of metastases at diagnosis, only the Clark level and fractal dimension of the nuclear chromatin were included as independent prognostic factors in the final regression model. (biomedcentral.com)
  • The increased nuclear fractal dimension found in the more aggressive melanomas is the mathematical equivalent of a higher complexity of the chromatin architecture. (biomedcentral.com)
  • So, there is strong evidence that the fractal dimension of the nuclear chromatin texture is a new and promising variable in prognostic models of malignant melanomas. (biomedcentral.com)
Complexes3
  • Chromatin dynamics is regulated by many factors, including histone tail modifiers, remodeling complexes, and histone chaperones ( Peterson and Almouzni, 2013 ). (molcells.org)
  • The core spliceosome component PRPF8 is essential for spliceosome assembly through its participation in ribonucleoprotein (RNP) complexes for splice-site recognition, branch-point formation and catalysis. (biomedcentral.com)
  • Together, these studies begin to define the molecular determinants of assembly of functionally diverse macromolecular histone chaperone complexes. (omicsdi.org)
Dynamics3
  • Chromatin dynamics is essential for maintaining genomic integrity and regulating gene expression. (molcells.org)
  • Interestingly, we detected a non-canonical inversion of collinear chromatin dynamics at the Abd-B gene, with the active histone domain decreasing in size. (biorxiv.org)
  • Incorporation of histone variants confers variability to the chromatin and expands the repertoire of epigenetic marks in a functional alphabet that controls genome plasticity and dynamics [ 4 ]. (aging-us.com)
Chaperones1
Repression3
  • We show that Bonus associates with the histone methyltransferase SetDB1 and the chromatin remodeler NuRD and depletion of either component releases Bonus-induced repression. (elifesciences.org)
  • Fig. 103: Schematic of the overall arrangement of the SMRT repression complex, a multivalent chromatin deacetylating machine. (esrf.fr)
  • Taken together, the crystal structure of the TBL1-NTD tetramer, the NMR structure of the SMRT:GPS2 heterodimer, along with mutagenesis and docking experiments give us considerable insight into the assembly of the core SMRT/NCoR:TBL1:GPS2:HDAC3 repression complex. (esrf.fr)
Regulates1
  • Sequential assembly of the human spliceosome on RNA transcripts regulates splicing across the human transcriptome. (biomedcentral.com)
Cell differentiation1
  • Interferon regulatory factor 4 (IRF4) and IRF8 regulate B, T, macrophage, and dendritic cell differentiation. (nih.gov)
Compaction1
  • Chromatin compaction protects the genome and maintains genomic integrity. (molcells.org)
Drosophila1
  • The early Drosophila embryo is an attractive system to study chromatinassembly in a developmental context. (ens-lyon.fr)
Vivo1
  • We provide the first direct determination that ASF1A and ASF1B play a role in the efficiency of nucleosome assembly in vivo in human cells. (omicsdi.org)
Functionally distinct1
  • Finally, our data indicate that the CAF-1-dependent pathway required for cohesion is functionally distinct from the Rtt101-Mms1-Mms22 pathway which functions in replicated chromatin assembly. (cell-stress.com)
Nucleus1
  • Eukaryotic cells compact their large genome into highly ordered chromatin structures within the nucleus. (aging-us.com)
Gene5
  • Change in gene expression associated with pancreatic cancer could be attributed to the variation in histone posttranslational modifications leading to subsequent remodeling of the chromatin template during transcription. (nebraska.edu)
  • Since the ubiquitous general transcription factors represent the ultimate targets of the various gene-specific factors, their characterization is essential. (rockefeller.edu)
  • Some of the general factors have been shown to be direct targets for specific regulatory factors, while more recent studies have implicated a number of general and gene-specific cofactors in the function of gene-specific activators. (rockefeller.edu)
  • These various classes of cofactors, as well as others (p300/CBP, STAGA) implicated in chromatin template modifications, offer important new mechanisms for gene regulation. (rockefeller.edu)
  • Transcriptional activation of the interferon-beta (IFN-beta) gene requires assembly of an enhanceosome containing ATF-2/c-Jun, IRF-3/IRF-7, and NFkappaB. (rcsb.org)
HIRA2
  • Structure of a human ASF1a-HIRA complex and insights into specificity of histone chaperone complex assembly. (omicsdi.org)
  • CAF-1 p60 also uses B domain-like motifs for binding to ASF1a, thereby competing with HIRA. (omicsdi.org)
Eukaryotic1
Vitro2
  • Cells resembling 2-cell-stage mouse embryos (totipotent cells) can be induced in vitro through downregulation of the chromatin-assembly activity of CAF-1 in embryonic stem cells. (wikipedia.org)
  • Here, we show that CAF-1 binds recombinant H3-H4 with 10- to 20-fold higher affinity than H2A-H2B in vitro, and H3K56Ac increases the binding affinity of CAF-1 toward H3-H4 2-fold. (elsevierpure.com)
Reorganization1
  • During the process of classification of the human agent, phylogenetic studies showed taxonomic disarray among organisms broadly referred to as ehrlichiae, and a careful reorganization now places those bacteria previously classified as E . phagocytophila , E . equi , and the HGE agent into a different genus as a single species, A . phagocytophilum ( Figure 2 ) ( 1 , 3 ). (cdc.gov)
Role of histone1
  • In such a crowded molecular picture, recent exciting insights have uncovered the role of histone variants as key regulators of the chromatin structure. (aging-us.com)
Complex6
  • p48 has roles outside of CAF-1, but when involved with the complex, it binds to H4. (wikipedia.org)
  • However, loss of function in p48 would alter how well the complex is able to chaperone chromatin, but would not stop it as a whole. (wikipedia.org)
  • CAF-1 forms a deadenylase complex with CCR4-Not, which should not be confused with the unrelated CCR4. (wikipedia.org)
  • The CAF-1/CCR4-Not complex cooperates with the release factor eRF3 and PABPC1 to shorten the poly(A) tail of mRNA during translation. (wikipedia.org)
  • In S. cerevisiae , origin licensing occurs by the assembly during G1 phase of a pre-replicative complex (pre-RC) at each potential origin. (nature.com)
  • In general, a more aggressive behaviour is usually found in genetically unstable neoplasias with a higher number of genetic or epigenetic changes, which on the other hand, provoke a more complex chromatin rearrangement. (biomedcentral.com)
Genome stability1
  • We dissected the role of CAF-1 in the maintenance of genome stability in ctf4 ∆ yeast cells. (cell-stress.com)
P1501
  • The CAF-1 p150 antobody was raised against full-length CAF-1 purified from human 293 cells. (fishersci.com)
20233
Structure3
  • Micrococcal nuclease digestion showed an altered chromatin structure in hPaf1/PD2-KD cells. (nebraska.edu)
  • Cryo-EM structure of the nucleocapsid-like assembly of respiratory syncytial virus. (ucsf.edu)
  • In the present study, the structural basis of nucleosome assembly activity in P. falciparum was addressed by determining and analysing the crystal structure of smaller NAP in the parasite called PfNapS. (biomedcentral.com)
Binds3
  • However, it is unknown how CAF-1 binds and delivers H3-H4 to the DNA. (elsevierpure.com)
  • A single CAF-1 molecule binds a cross-linked (H3-H4) 2 tetramer, or two H3-H4 dimers that contain mutations at the (H3-H4) 2 tetramerization interface. (elsevierpure.com)
  • These results suggest that CAF-1 binds to two H3-H4 dimers in a manner that promotes formation of a (H3-H4) 2 tetramer. (elsevierpure.com)
Biological4
  • Our studies are also directed toward the mechanisms by which these factors in turn are regulated in the various biological responses. (rockefeller.edu)
  • Biological assembly 1 assigned by authors. (rcsb.org)
  • The mechanisms that underlie and dictate the different biological outcomes of E2F-1 activity have yet to be elucidated. (embl.de)
  • Our results define an exquisite level of precision in the reader-writer interplay that governs the biological outcome of E2F-1 activity. (embl.de)
Histone octamer1
  • A histone chaperone that facilitates nucleosome assembly by mediating the formation of the histone octamer and its transfer to DNA. (lookformedical.com)
REMODELING2
  • Chromatin Remodeling of Colorectal Cancer Liver Metastasis is Mediated by an HGF-PU.1-DPP4 Axis. (duke.edu)
  • Here it is shown that metastatic cells undergo specific chromatin remodeling in the liver. (duke.edu)
Modifications1
  • Histone modifications regulate pioneer transcription factor cooperativity. (ucsf.edu)
Conformation1
  • The mechanisms effecting establishment, maintenance, and modification of that specific physical conformation of CHROMATIN determining the transcriptional accessibility or inaccessibility of the DNA. (lookformedical.com)
Localization1
  • SUMOylation influences Bonus's subnuclear localization, its association with chromatin and interaction with SetDB1. (elifesciences.org)
Stepwise1
  • Stepwise sequential assembly of spliceosome components on these pre-mRNA motifs executes splicing reactions. (biomedcentral.com)
SMRT1
  • In this study we sought to understand the mode of assembly of SMRT with TBL1 and GPS2. (esrf.fr)
Acetylation1
P1051
  • chromatin assembly factor-1 p105 subu. (nig.ac.jp)
Repressive1
  • Recruitment of Bonus to chromatin leads to silencing associated with accumulation of the repressive H3K9me3 mark. (elifesciences.org)