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Time FactorsMolecular Sequence DataAlgorithmsKineticsAmino Acid SequenceModels, BiologicalGelsChromatography, High Pressure LiquidChromatography, AffinityChromatography, GelChromatographyChromatography, GasChromatography, Ion ExchangeChromatography, Thin LayerChromatography, LiquidChromatography, DEAE-CelluloseMolecular WeightElectrophoresis, Gel, Two-DimensionalChromatography, AgaroseElectrophoresis, Polyacrylamide GelMass SpectrometryGas Chromatography-Mass SpectrometryChromatography, Reverse-PhaseElectrophoresis, Gel, Pulsed-FieldHydrogen-Ion ConcentrationChromatography, PaperElectrophoresis, Agar GelSepharoseIsoelectric FocusingSubstrate SpecificityAmino AcidsEscherichia coliTandem Mass SpectrometryBase SequenceSpectrophotometry, UltravioletChemistryChemical PhenomenaCattleRabbitsChemical FractionationPeptide FragmentsSpectrometry, Mass, Electrospray IonizationTemperatureProtein BindingMethodsTrypsinLiverCarbohydratesElectrophoresisDrug StabilityRecombinant ProteinsCountercurrent DistributionHydrolysisChromatography, Micellar Electrokinetic CapillaryPeptidesMagnetic Resonance SpectroscopyCloning, MolecularCarbohydrate SequenceHydroxyapatitesBacterial ProteinsIndicators and ReagentsBinding SitesIsoelectric PointOligosaccharidesCell LineCentrifugation, Density GradientChemical PrecipitationHot TemperatureSpecies SpecificitySwineMacromolecular SubstancesRadioimmunoassayPolysaccharidesSolubilityDNAReproducibility of ResultsAmmonium SulfateImmunodiffusionUltracentrifugationIsoenzymesMicrochemistryReference StandardsProteinsCalibrationImmunoelectrophoresisLectinsBiological AssayCarrier ProteinsFatty AcidsSequence Homology, Amino AcidGlycoproteinsCarbohydrate ConformationRats, Inbred StrainsStereoisomerismCells, CulturedProteomicsBrain ChemistryAcetonitrilesPhysicochemical PhenomenaCell Membrane
Thin Layer ChromatoHPLCPurificationPermeation chromatography systemSilica gel platesFurther purifiedFiltrationScanning electron mAffinityPrincipleSubstrateStationaryElectrophoresisPolymersRetentionChromatographicSugars1978HydrophilicAnalytical techniqueColumnWater-solublePhasesPoreLiquidLaboratoriesPhysicochemical propertiesProteinsInteractionSpeciesCompoundsCleanupMixturesChemicalLigninSizeDescribesExcipientsTechniqueColumnsInstrumentAnalysisMassStabilityFunctionalStructuralSampleGeneralGuideSeparate plantContent
Thin Layer Chromato4
  • The first one is Thin Layer Chromatography (TLC), which is carried out on an aluminum sheet of silica gel with the help of an eluent. (databasefootball.com)
  • The principle of flash column chromatography and thin-layer chromatography is the same, but it can be used for the separation of preparative substances. (flashcolumns.com)
  • This thin layer chromatography (HPTLC) allows to obtain a qualitative image characteristic of each plant, allowing to detect adulterations in a very short time. (sorianatural.com)
  • A similar phenomenon has been noted in the separation of MAG and DAG (as acetates) by thin layer chromatography on silica gel G plates. (usda.gov)
HPLC8
  • In most laboratories, chromatography is performed using high-performance liquid chromatography (HPLC) or gas chromatography (GC) . (coleparmer.com)
  • High performance liquid chromatography (HPLC), a form of column chromatography, can be performed in one of two ways. (idtdna.com)
  • The type of HPLC selected is dependent on oligo sequence composition and characteristics. (idtdna.com)
  • The PFY-147-EPS was found to be a levan containing a backbone of 2,6-substituted ß-fructoses by high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) and Fourier transform infrared (FT-IR) spectroscopy. (bvsalud.org)
  • Different methods were used to characterise the gels: HPLC, gas chromatography, pH, visual observation and optical microscopy. (bmj.com)
  • During development of a silver ion HPLC method to determine the structural integrity of synthesized monoacyl (MAG) and diacylglycerols (DAG), the retention characteristics of fatty acid methyl esters (FAME) and triacylglycerols (TAG) were found to be influenced not only by the number and/or configuration of the double bonds in the molecules, but also by the number of carbon atoms in the fatty acid (FA) chains. (usda.gov)
  • From gas chromatography and HPLC to ion-exchange, gel permeation and affinity, there are various different types of chromatography to choose from. (chromatographytoday.com)
  • Combined with TLC bioautography assay and HPLC comparison, the four antioxidant components separated by silica gel chromatography and further purified by Sephadex LH-20 were determined to be caffeic acid, rosmarinic acid, luteolin and apigenin, respectively. (rsc.org)
Purification12
  • Gel permeation chromatography system working principle , on which the gel purification system works, separates and collects complex samples according to the size of the solute molecules. (antiteck.com)
  • A gel purification system with multiple specifications of stainless steel purification column and glass purification column is available, and the glass purification column has a spiral mouth design, which is convenient to adjust the column length. (antiteck.com)
  • Chromatography can be used in a myriad of applications from analyzing miniscule samples to production-scale use as a purification step. (coleparmer.com)
  • Partial purification of the toad PRL receptor could be achieved by affinity chromatography. (eurekamag.com)
  • And it is a fast and inexpensive technology compared with traditional chromatography, which is widely used in drug research, sample purification, and purification of natural products currently. (flashcolumns.com)
  • The characteristics of low-cost and simplicity result in its irreplaceable in the separation and purification. (flashcolumns.com)
  • 2. Use column chromatography above 100 grams to prepare purification technology. (flashcolumns.com)
  • A nearly 136-fold enzyme purification was obtained from chicken erythrocyte by acetone precipitation, ethanol-chloroform treatment, CM-cellulose and Sephadex G-200 chromatography. (tubitak.gov.tr)
  • The CHO cell line secretes recombinant factor IX into a defined cell culture medium that does not contain hormones, and the recombinant factor IX is purified by a chromatography purification process that does not require a monoclonal antibody step. (globalrph.com)
  • The principle is to use the characteristics of the protein surface to make it adsorbed on the gel column to achieve separation and purification The purpose of protein. (sepuretech.com)
  • An orthogonally (80.3%) preparative two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography method has been established for the isolation and purification of flavonoids from Saxifraga tangutica. (virginia.edu)
  • Then, the coeluted flavonoid fractions were selected for further purification via reversed-phase liquid chromatography with the parent ion peak of quercetin (303), kaempferol (287), or isorhamnetin (317). (virginia.edu)
Permeation chromatography system2
Silica gel plates2
Further purified1
Filtration7
Scanning electron m1
Affinity3
  • The editor today will talk to you about His-Tag affinity chromatography packing material. (sepuretech.com)
  • According to the different binding groups, Ni2 + affinity chromatography columns can be divided into two categories-Ni-IDA and Ni-NTA. (sepuretech.com)
  • For more inquiries about His-Tag affinity chromatography packing, please continue to pay attention to the official website of Suzhou Sepure Instrument Co., Ltd. www.sepuretech.com! (sepuretech.com)
Principle3
  • It is a relatively traditional separation technique, based on the principle of gel permeation chromatography to separate and collect complex samples according to the size of the molecular volume, which can effectively remove the large molecular matrix and small molecules in the sample, improve the sensitivity and accuracy of subsequent analysis, and extend the service life of analytical instruments. (antiteck.com)
  • Today, most applications of chromatography are generally less colorful but the underlying principle of separation remains unchanged. (coleparmer.com)
  • Gel permeation chromatography its principle instrument. (web.app)
Substrate2
  • Besides, there is also the synthesis of the native oligocellulose with the help of the natural substrate with the aim of comparing the characteristics of the synthesized vinyl-based oligocellulose with the natural ones. (databasefootball.com)
  • As a consequence, most methods of flash chromatographic application in the literature are developed using silica gel as the separation substrate. (flashcolumns.com)
Stationary9
  • At its core, chromatography is the interaction between a mobile phase (which carries the mixture being separated) and a stationary phase (which performs the separation). (coleparmer.com)
  • Most chromatography methods have an inert mobile phase that carries the analyte through a long stationary phase housed inside a column. (coleparmer.com)
  • The stationary phase is designed to separate the components of the analyte based on some defined characteristic, which causes some molecules to migrate through the stationary phase more slowly and others to pass through more quickly. (coleparmer.com)
  • For example, in gel permeation chromatography, special inert beads are used as the stationary phase, and the mobile phase carries the analyte past those beads. (coleparmer.com)
  • Bare silica gel is an acceptable stationary phase. (tsijournals.com)
  • The chemical attachment of suitable groups, usually C18 alkyl groups, onto a carrier, primarily silica gel, produces the practically applicable volume of LC stationary phase. (tsijournals.com)
  • An inert gas carries the sample through the column, and the partitioning of methoxychlor between the mobile and stationary phases gives it a characteristic retention time that is used to identify it. (cdc.gov)
  • In size exclusion chromatography, the stationary phase is. (web.app)
  • Gel permeation chromatographysize exclusion chromatography as we have seen, gpcsec is a type of lc and so solid stationary and liquid mobile phases are again used. (web.app)
Electrophoresis6
  • Separation involves techniques such as gel electrophoresis or liquid chromatography to separate proteins based on their size, charge, or other properties. (proprofs.com)
  • SDS-gel electrophoresis results indicated that chicken erythrocyte catalase consists of four apparently identical subunits, with a molecular weight of around 57.5 kDa. (tubitak.gov.tr)
  • Polyacrylamide gel electrophoresis (PAGE) separates full-length product from shorter species based on electric charge. (idtdna.com)
  • The enzymatic oxidation was characterized by Raman, ultraviolet-visible, electron paramagnetic resonance, Fourier transform infrared spectroscopy, transmission electron microscopy, atomic force microscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and gas chromatography-mass spectrometry. (cdc.gov)
  • RIXUBIS is predominantly a single component by sodium dodecyl sulfate-polyacrylamide gel electrophoresis evaluation. (globalrph.com)
  • Subsequently, phosphatidylcholine, lysophosphatidyl¬choline, sphingo-myelin, ceramide, phosphatidylethanolamine, phosphatidylethanolamine-based plasmalogen, cholesterol and cholesteryl ester content of the separated lipoproteins was quantified by electrospray ionization tandem mass spectrometry (ESI-MS/MS). Results: Analysis of FPLC fractions with polyacrylamide gel electrophoresis demonstrated that albumin partially co-elutes with HDL fractions. (uni-regensburg.de)
Polymers5
  • Synthetic polymers are subjected to two-dimensional liquid chromatography, or 2D-LC. (tsijournals.com)
  • In this approach, the existence of surface adsorption and enthalpic partition as two similar but distinct enthalpic retention mechanisms in high-performance liquid chromatography of polymers was indirectly demonstrated. (tsijournals.com)
  • Gel permeation chromatography on branched polymers. (web.app)
  • Gel permeationsize exclusion chromatography 5 chapter 2 gpcsec overview 6 polymers 6 size matters 6 how does gpcsec work 7 who uses gpcsec, what for and why 8 calibrations 8. (web.app)
  • Moore, of the dow chemical company, published his work on the preparation of gel permeation chromatography gpc and changed how scientists studied synthetic polymers and. (web.app)
Retention2
  • Basic information on retention mechanisms in synthetic polymer liquid chromatography is provided. (tsijournals.com)
  • Researchers will know their characteristics, such as retention times and mass spectra, so they can target them specifically. (chromatographytoday.com)
Chromatographic6
  • At 35 °C, two separate liquid chromatographic critical ranges were discovered using non-end-capped silica gel C-18 column packing, poly (methyl methacrylate)s (PMMAs), and mixed eluents including acetonitrile (ACN) and toluene. (tsijournals.com)
  • Flash chromatography is a kind of fast chromatographic separation mode, which separates substances by using optimized pre-packaged medium and low-pressure columns. (flashcolumns.com)
  • Gel permeation chromatography gpc is a chromatographic technique in which the separation obtained is a function of molecular size. (web.app)
  • Evaluation of gel permeation chromatographic techniques. (web.app)
  • Gel permeation chromatography gpc is a column separation technique based on a nonionic molecularsieve effect and, in contrast to other chromatographic. (web.app)
  • Initially, flavonoids were enriched by means of a middle-pressure chromatographic tower (containing middle chromatogram isolated gel). (virginia.edu)
Sugars1
  • Next, the component sugars of sample P were investigated by gas chromatography, and the results are shown in Figure 2 . (scirp.org)
19781
Hydrophilic1
  • These results established that the off-line two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography technique was efficient for the isolation of flavonoids from Saxifraga tangutica. (virginia.edu)
Analytical technique1
  • Instrumentation available waters gpc viscotek gpc max gel permeation chromatography gpc from eag website gel permeation chromatography gpc is an analytical technique for the. (web.app)
Column11
  • The column packing gel has pores of different sizes, and when component molecules of different sizes enter the gel with the mobile phase, the smaller the molecules are the deeper they enter the micropores and the longer they are retained in the gel, so they can be eluted in order of molecular size. (antiteck.com)
  • Flash column chromatography, also known as a flash column, is a fast separation method, but it does not sacrifice resolution as others have said. (flashcolumns.com)
  • In the general column chromatography process, it is usually accompanied by lateral diffusion, which will greatly reduce the resolution, so when walking the column, you should try to shorten the column chromatography time, especially to avoid the column overnight. (flashcolumns.com)
  • Flash column chromatography is a fast and usually easy method to separate complex mixtures. (flashcolumns.com)
  • Because we use compressed air to push the solvent through the column, it is called flash column chromatography. (flashcolumns.com)
  • Similarly, VLC (Vacuum Column Chromatography) is also a very good column chromatography technique. (flashcolumns.com)
  • In addition to the transformation of the synthetic route of the process, efficient column chromatography amplification technology is also a very critical step. (flashcolumns.com)
  • Theory of separation a column is made up of swollen gel particles and the solvent used to swell the gel in a suitable tubular container. (web.app)
  • How Do You Prepare Column Chromatography? (chromatographytoday.com)
  • How Does Column Diameter Affect Column Chromatography? (chromatographytoday.com)
  • In the first dimension, a XION preparative column was used to separate the flavonoid fractions under the guidance of characteristic ultraviolet absorption spectra of flavonoids and nine flavonoid fractions were obtained. (virginia.edu)
Water-soluble1
  • To screen and characterize the analgesic components from Marasmius androsaceus , the water-soluble extracts from the mycelium of M. androsaceus were isolated by ethanol precipitation followed by macroporous resin chromatography and activity-based fractionation. (scirp.org)
Phases1
  • Regardless of how it is used, the overall effectiveness of chromatography largely depends on choosing the right technique and phases to use within that technique. (coleparmer.com)
Pore1
  • Composed of semipermeable, porous polymer gel beads with well defined range of pore sizes. (web.app)
Liquid6
Laboratories1
Physicochemical properties2
  • Next, the physicochemical properties and characteristics of these purified glycopeptides were studied. (scirp.org)
  • Toward this end, as we reported herein, we separated and screened the water extract from the mycelium of M. androsaceus for the effective components, and subsequently, purified the active components to study their physicochemical properties and chemical characteristics. (scirp.org)
Proteins1
  • These three steps are essential in understanding the composition and characteristics of proteins within a sample. (proprofs.com)
Interaction1
  • This report explores the use of high performance gel permeation chromatography hpgpc as a means of studying asphalt composition and their intermolecular interaction. (web.app)
Species2
  • This technique can also be used to identify the different plant species by searching for their characteristic DNA, and to find dangerous microorganisms in the products by searching for their DNA, etc. (sorianatural.com)
  • VLDL, LDL and HDL displayed characteristic lipid class and species pattern. (uni-regensburg.de)
Compounds2
  • A variety of chromatography equipment is also available that can provide an accurate identification of known compounds or in-depth data about unknown compounds. (coleparmer.com)
  • These UNIPLATES are impregnated with between 5% and 20% silver nitrate which modifies the separation characteristics of the adsorbent layer to permit increased discrimination of certain compounds, particularly those containing carbon-carbon double bonds. (milesscientific.com)
Cleanup1
  • The traditional pretreatment process is time-consuming and solvent consumption is high, so the large-scale application of gel cleanup system is an inevitable trend. (antiteck.com)
Mixtures1
  • Chromatography is a technique for separating mixtures into their constituent parts. (coleparmer.com)
Chemical2
  • The chemical composition of the extracted polysaccharide contains fructose and glucose with protein-free identified by gas chromatography-mass spectrometer (GC-MS) method. (bvsalud.org)
  • Moore, of the dow chemical company, published his work on the preparation of gel permeation chromatography. (web.app)
Lignin4
  • Deep eutectic-like solvent-assisted isolation of lignin from Pinus densiflora and its characteristics ," BioResources 17(4), 5600-5611. (ncsu.edu)
  • Three types of DESs were used, and the effects of treatment temperature (100, 110, and 130 °C) and time (6, 12, and 24 h) on lignin yield and its characteristics were studied. (ncsu.edu)
  • This information was then correlated with the polymeric properties of the materials (glass transition temperature ( T g), molecular weight characteristics, and thermal stability) for a series of selectively and progressively derivatized softwood kraft lignin samples. (ncsu.edu)
  • Softwood kraft lignin was highly susceptible to thermally induced reactions that caused its molecular characteristics to be severely altered with the concomitant formation of irreversible cross-linking. (ncsu.edu)
Size6
Describes2
  • This test guideline describes the gel permeation chromatography gpc. (web.app)
  • Conclusions This study describes a new gel formulation to improve skin penetration using various excipients to promote skin tolerance. (bmj.com)
Excipients1
  • Analyzing pharmaceutical tablet and capsule excipients by gel permeation. (web.app)
Technique1
  • The technique of gel permeation chromatography gpc was utilized in analysis of paraffinic wax samples generated via the fischertropsch synthesis in a slurry reactor. (web.app)
Columns2
  • The most used common filler for flash columns is silica gel. (flashcolumns.com)
  • Shimpack gpc 80m mixed gel columns 80mc, 80md mixed gel columns that produce linear calibration curves over a wide range of molecular weights. (web.app)
Instrument1
Analysis1
Mass1
  • However, in the presence of a fluctuating second or even third molecular characteristic, GPC/SEC cannot provide information regarding the polymer molar mass. (tsijournals.com)
Stability2
  • This study provides, for the first time, detailed stability data for a hydro-alcoholic rapamycin gel. (bmj.com)
  • Stability of serum obtained with tubes containing separating gel: 48 hours at 2-8°C (note the data provided by the tube manufacturer). (cdc.gov)
Functional2
  • Functional characteristics of GPC system. (antiteck.com)
  • Its amino acid sequence is identical to that of the Ala-148 allelic form of plasma derived factor IX, and its structural and functional characteristics are similar to those of plasma derived factor IX. (globalrph.com)
Structural1
  • Maca (Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2% were obtained to investigate their structural characteristics and antifatigue effect in vivo. (nih.gov)
Sample1
General1
  • In general, the characteristics of implanted and in vitro cycled sacs were similar to their control sacs. (psu.edu)
Guide1
Separate plant1
Content1
  • It is proposed that the fingerprint with the four characteristic peaks as common peaks and total phenolic content determination were necessary for the quality control of this plant and should be adopted in its quality standards. (rsc.org)