DiscoideumYeast and mammalian cellsLipid dropletProteinsIsoformsDrosophilaCellsPathwaysChemotaxisFungiEnzymeDevelopmentalStrainSpeciesDevelopmentBacteriaEarlyIdentificationKindDomainsSocial amoebaAmoeba Dictyostelium discoideumDiscoideum and mammalianCultured mammalian cellsUnderlying mammalian cellCaenorhabditisMacropinocytosisGeneticChemoattractantMotileAbstractGenesProtein-coupledCellularSpeciesBiologyAssaysTaxonomicRegulationModelModelsStructureHumanNetworkCell cultureShown
Discoideum13
- In the model organism Dictyostelium discoideum , loss of the CLN5 homolog, cln5 , impacts various cellular and developmental processes including cell proliferation, cytokinesis, aggregation, cell adhesion, and terminal differentiation. (frontiersin.org)
- We present a detailed sequence analysis of the 10 formins (ForA to J) identified in the genome of the social amoeba Dictyostelium discoideum . (biomedcentral.com)
- Association of calmodulin and an unconventional myosin with the contractile vacuole complex of Dictyostelium discoideum. (rupress.org)
- Dictyostelium discoideum rp1024. (expasy.org)
- The development of Dictyostelium discoideum to the multicellular stage and formation of fruiting bodies is largely determined by cAMP. (biomedcentral.com)
- These invertebrate systems such as Caenorhabditis elegans , Drosophila melanogaster, Dictyostelium discoideum and Galleria mellonella offer a number of advantages over mammalian vertebrate models, predominantly because they allow the study of strains without the ethical considerations associated with mammalian studies [ 11 - 13 ]. (biomedcentral.com)
- 2013). Functional properties of five Dictyostelium discoideum P2X receptors. (gozmusic.org)
- The major research interest of my laboratory is in the processes of chemotaxis and development in the cellular slime mould Dictyostelium discoideum. (ncku.edu.tw)
- Kim JY, Haastert PV, Devreotes PN (1996) Social senses: G-protein-coupled receptor signaling pathways in Dictyostelium discoideum . (springer.com)
- This project suggests aprocess that uses the social amoeba, Dictyostelium discoideum, to conduct pre-liminary screening of agrochemicals for developmental toxicity. (manchester.ac.uk)
- A novel DNHE1 in Drosophila that binds an ERM ortholog, and a novel DdNHE1 in Dictyostelium discoideum that is necessary for cell polarity and chemotaxis were identified, suggesting that actin anchoring by NHE1 and a requirement for NHE1 in directional cell movement are evolutionarily conserved. (grantome.com)
- In Dictyostelium discoideum , two PPK activities have been originally reported, one with sequence similarity to the bacterial PPK1 that was acquired through horizontal gene transfer [ 7 ], and a novel DdPPK2 activity, that seems to be a complex of three actin-related proteins: Arp1, Arp2, and an unreported Arpx [ 8 ]. (silverchair.com)
- This video is composed of a sequence of films created by John Tyler Bonner in the 1940s to show the life cycle of the cellular slime mold Dictyostelium discoideum. (asu.edu)
Yeast and mammalian cells1
- similar proteins in yeast and mammalian cells have been implicated in vesicle movement. (rupress.org)
Lipid droplet4
- Figure 1A shows that lipid droplet formation in Dictyostelium has some qualities also observed in mammalian cells (34). (acthreceptor.com)
- Due to the fact Dictyostelium Net4 is identified on lipid droplets when the medium is supplemented with fatty acid (Fig. 5D), we also tested the localization for the human NET4 protein and, certainly, discovered this house conserved from amoebae to humans (Fig. 5E and F). Dual localization of lipid droplet proteins. (calcimimeticagent.com)
- Looking at online resources for the expression on the genes we've got confirmed above as lipid droplet components of Dictyostelium, we discover that all of them are expressed in vegetatively expanding cells, i.e., within the absence of fatty acid addition. (calcimimeticagent.com)
- Christoph Thiele (Bonn, Germany) generously offered the lipid droplet-specific probe LD540, and Eric Schirmer (Edinburgh, Uk) created the mammalian NET4 plas. (calcimimeticagent.com)
Proteins1
- Aim 2 addresses how the two functions of NHE1 in ion translocation and actin anchoring coordinately remodel cell-substrate adhesions in mammalian fibroblasts by asking whether actin anchoring by NHE1 is necessary for integrin activation or for subsequent steps in the assembly of focal adhesion-associated proteins, and whether ion translocation by NHE1 promotes focal adhesion disassembly through a pH dependent disruption of the integrin-cytoskeleton linkage. (grantome.com)
Isoforms2
- This enzyme catalyses the following chemical reaction (1) ATP + 1D-myo-inositol hexakisphosphate(Phytic acid) ⇌ {\displaystyle \rightleftharpoons } ADP + 1D-myo-inositol 5-diphosphate 1,2,3,4,6-pentakisphosphate (2) ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate ⇌ {\displaystyle \rightleftharpoons } ADP + 1D-myo-inositol diphosphate tetrakisphosphate (isomeric configuration unknown) Three mammalian isoforms are known to exist. (wikipedia.org)
- Our analysis allows some preliminary insight into the functionality of Dictyostelium formins: all isoforms might display actin nucleation activity and, with the exception of ForI, might also be susceptible to autoinhibition and to regulation by Rho GTPases. (biomedcentral.com)
Drosophila1
- GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). (cellsignal.com)
Cells13
- The mechanisms underlying the appearance of asymmetry between cells in the early embryo and consequently the specification of distinct cell lineages during mammalian development remain elusive. (nature.com)
- We use cell biological, biochemical, biophysical and genetic approaches and diverse model systems, such as yeast, Dictyostelium, and mammalian cells, to elucidate the molecular principles underlying the organization of the cytoplasm. (tu-dresden.de)
- mAbs specific for calmodulin were used to examine the distribution of calmodulin in vegetative Dictyostelium cells. (rupress.org)
- When membranes from Dictyostelium cells were fractionated on equilibrium sucrose density gradients, calmodulin cofractionated with alkaline phosphatase, a cytochemical marker for contractile vacuole membranes, at a density of 1.156 g/ml. (rupress.org)
- During early differentiation of Dictyostelium the attractant cAMP is released periodically to induce aggregation of the cells. (biomedcentral.com)
- In mammalian cells, as many as twenty types of protein have been reported to bind to activated Cdc42 [ 2 ]. (biomedcentral.com)
- About ten years ago, Miura and coworkers had fused the sequence from the Dictyostelium homologue of perilipin (Plin) to GFP and expressed the construct in mammalian CHO cells, exactly where it localized to lipid droplets (35). (acthreceptor.com)
- In order to receive a Aurora B Inhibitor Species marker for biochemical preparation of lipid droplets, we constructed a GFP-Plin fusion for the expression in Dictyostelium cells. (acthreceptor.com)
- The phenotypic effects of the drugs duringdevelopment were observed by growing Dictyostelium cells on agar to inducedevelopment. (manchester.ac.uk)
- Phosphatidylinositol 4,5 bisphosphate (PIP2) is the most abundant phosphoinositide in mammalian cells. (mechanobio.info)
- Studies in Aim 1 will determine how NHE1 regulates membrane protrusions and actin polymerization in mammalian fibroblasts by asking how localized H+ fluxes by NHE1 promote cell protrusion, the formation of actin free barbed ends, and the spatially restricted activation of Rac and Cdc42 at the leading edge of migrating cells. (grantome.com)
- Secondly, the low sensitivity and specificity of available polyP detection methods make it difficult to confidently ascertain polyP presence in mammalian cells, since in higher eukaryotes, polyP exists in lower amounts than in yeast or bacteria. (silverchair.com)
- Scientists have applied somatic cell nuclear transfer to clone human and mammalian embryos as a means to produce stem cells for laboratory and medical use. (asu.edu)
Pathways1
- To address the possibility that activation of phosphatidylinositol-3-kinase (PI3K) and the mammalian target of rapamycin (mTOR/FRAP), represents one of these pathways, we have examined the effect of simultaneous inhibition of the Ras-MAPK and PI3K-mTOR pathways on transformation of CEF by v-Src. (embl.de)
Chemotaxis1
- Aim 3 focuses on why DdNHE1 is necessary for Dictyostelium chemotaxis by asking whether it generates localized H+ efflux, whether it maintains polarity by acting on signals at leading or trailing edges of the cell, and whether genetic screens identify DdNHE1 effectors mediating polarity and chemotaxis. (grantome.com)
Fungi1
- Krishnan A, Almen MS, Fredriksson R et al (2012) The origin of GPCRs: identification of mammalian like Rhodopsin, Adhesion, Glutamate and Frizzled GPCRs in fungi. (springer.com)
Enzyme2
- Previous work in mammalian models reported altered lysosomal enzyme activity due to mutation or loss of CLN5 . (frontiersin.org)
- Firstly, the mammalian enzyme responsible for the synthesis of this linear biopolymer is unknown. (silverchair.com)
Developmental2
- This new method of screeningnew agrochemicals for developmental toxicity could decrease the number ofunsuitable compounds that reach mammalian testing, saving both time andmoney. (manchester.ac.uk)
- This theme can be modified for specific developmental programs such as the unequal cleavages of mammalian female meiosis. (biomedcentral.com)
Strain1
- Both the growth experiment in media and the development ex-periment were also conducted using a mutant strain of Dictyostelium as a proofof principle experiment. (manchester.ac.uk)
Species1
- The all-natural food sources of Dictyostelium, i.e., numerous species of bacteria, contain fatty acids largely in esterified kind. (axlinhibitor.com)
Development1
- Figure 2: The self-organization theory of early mammalian development. (nature.com)
Bacteria1
- The effects these compounds haveon Dictyostelium cell growth was studied in axenic media and on SM agarplates in the presence of bacteria. (manchester.ac.uk)
Early1
- These findings can be integrated in a new unified framework that regards the early mammalian embryo as a self-organizing system. (nature.com)
Identification1
- Construction of simple and efficient siRNA validation systems for screening and identification of effective RNAi-targeted sequences from mammalian genes. (ncku.edu.tw)
Kind1
- The function ascribed to mammalian NET4 so far is based on modest interfering RNA (siRNA) research, which in-dicate that loss of NET4 slows down the cell cycle, even top to premature senescence, based on the cell kind studied (24). (calcimimeticagent.com)
Domains1
- To establish the relationship between formins of Dictyostelium and other organisms we constructed a phylogenetic tree based on the alignment of FH2 domains. (biomedcentral.com)
Social amoeba2
- The social amoeba or cellular slime mould Dictyostelium discoideum is a "professional" phagocyte that has long been recognized for its value as a biomedical model organism, particularly in studying the actomyosin cytoskeleton and chemotactic motility in non-muscle cells. (nih.gov)
- Subsequently, he carried out two postdoctoral stays, first at the University of San Diego (USA) and then at the IIBM (Spain), studying the developmental mechanisms of the social amoeba Dictyostelium discoideum (1995-2006). (autourdubpan.fr)
Amoeba Dictyostelium discoideum2
- The unicellular amoeba Dictyostelium discoideum embarks on a developmental program upon starvation. (tmc.edu)
- This group proposes to use the amoeba Dictyostelium discoideum, as a biological model to study the biosynthesis of Iron-Sulfur Clusters ([Fe-S]) at the molecular, cellular and organism levels. (curefa.org)
Discoideum and mammalian1
- One of Dr. Xu's primary areas of research focuses on chemoattractant GPCR-mediated chemotaxis using both the model organism D. discoideum and mammalian neutrophils. (nih.gov)
Cultured mammalian cells1
- In 2007 he obtained a permanent position at the IIBM (Spain) and focused his interest on the mechanisms of autophagy, using Dictyostelium and cultured mammalian cells as biomedical models of rare diseases, specifically chorea-acanthocytosis and BPAN . (autourdubpan.fr)
Underlying mammalian cell1
- The processes underlying mammalian cell migration are believed to be consistent with those of (non- spermatozooic ) locomotion . (wikipedia.org)
Caenorhabditis1
- [ 29 , 53 ] Random mutagenesis of the A. baumannii ATCC 17978 strain led to identification of several mutants in six different pathogenicity islands, which presented attenuated virulence toward the non-mammalian models Caenorhabditis elegans and Dictyostelium discoideum . (medscape.com)
Macropinocytosis2
- Here, existing techniques have been adapted to allow high-throughput, single-cell resolution measurement of macropinocytosis in the macropinocytosis model organism Dictyostelium discoideum using flow cytometry. (jove.com)
- This method can help answer key questions in the Dictyostelium and macropinocytosis fields such as whether a particular gene, protein or molecular pathway is involved in macropinocytosis. (jove.com)
Genetic1
- His studies of the slime mold Dictyostelium provided genetic proof that myosin is required for cell morphogenesis and cytokinesis. (nih.gov)
Chemoattractant2
- Both Dictyostelium and neutrophils sense chemoattractant gradients over a huge concentration range through a cellular phenomenon known as adaptation. (nih.gov)
- In Dictyostelium, binding of the chemoattractant cAMP to its G protein coupled receptor activates various effectors including the adenylyl cyclase, ACA, which converts ATP into cAMP. (nih.gov)
Motile1
- However, Dictyostelium combines these with a unique life style, with motile unicellular and multicellular stages, and multiple cell types that offer for study an unparalleled variety of phenotypes and associated signalling pathways. (nih.gov)
Abstract1
- abstract = "Differentiation-inducing factors (DIFs) are morphogens which induce cell differentiation in Dictyostelium. (elsevierpure.com)
Genes2
- however, the role of these genes in mammalian virulence were not assessed. (medscape.com)
- First, they have explored the D. discoideum genome looking for genes corresponding to the subunits that constitute the molecular machinery for Fe-S cluster assembly and, based on the structure of the mammalian supercomplex and amino acid conservation profiles, inferred the full functionality of the amoeba machinery. (curefa.org)
Protein-coupled1
- Both Dictyostelium and neutrophil use G protein-coupled receptors (GPCRs) to sense and move toward higher concentrations of extracellular chemoattractants. (nih.gov)
Cellular1
- The phenotypic richness and tractability of Dictyostelium should provide a fertile arena for future exploration of NDPK's cellular roles. (nih.gov)
Species1
- The phylogenetic analysis performed in our study also clearly demonstrates that the emergence of the family of seven P2X channels in human and other mammalian species was a relatively recent evolutionary event that occurred subsequent to the split between vertebrates and invertebrates. (biomedcentral.com)
Biology1
- Mammalian Biology , 78 (1), 1-6. (au.dk)
Assays1
- To study mammalian muscle contraction, he developed in vitro assays for ATP-dependent movement of purified myosin on actin filaments. (nih.gov)
Taxonomic1
- Taxonomic patterns in the zoonotic potential of mammalian viruses. (alexwashburne.com)
Regulation2
- GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). (cellsignal.com)
- Structure, mechanism, and regulation of mammalian adenylyl cyclase. (embl.de)
Model1
- She further expanded her research from the model organism to mammalian neutrophils and breast cancer. (nih.gov)
Models1
- In certain instances, we extend studies to mammalian models. (nih.gov)
Structure1
- Dictyostelium has also contributed significantly to our understanding of NDP kinase, as it was the Dictyostelium enzyme whose structure was first determined and related to enzymatic activity. (nih.gov)
Human1
- Combining computational simulation and experimental verification, Dr. Xu's research proposed and identified locally recruited Ras inhibitors, C2GAP1 and CAPRI in Dictyostelium and human neutrophils, respectively, as essential players in GPCR-mediated Ras adaptation, gradient sensing, and cell sensitivity. (nih.gov)
Network1
- History The contractile vacuole (CV) network of Dictyostelium is composed of pipes and bladders. (ampkpathway.com)
Cell culture1
- Expertise in cell culture and husbandry of mammalian and dicty cell cultures. (nih.gov)
Shown1
- We have also previously shown that the collection and release of ACA exosomes is essential for cell streaming and chemotactic signal relay during Dictyostelium cell aggregation. (nih.gov)