• Dictyostelium is the simplest organism known to utilize STATs, although the STATs are probably activated differently than in other organisms because Dictyostelium does not have classical receptor-tyrosine kinases. (highveld.com)
  • The goal is to establish Dictyostelium as a new model organism to study the functional role of prion-like proteins and their interaction with the proteostasis machinery. (mpi-cbg.de)
  • The haploid social amoeba Dictyostelium discoideum is a well-defined model organism which is amenable to diverse biochemical, cell biological, and molecular genetic approaches. (asmscience.org)
  • Studies using social amoeba Dictyostelium discoideum revealed that a complex signal transduction network of parallel pathways amplifies the response to chemoattractants, and leads to biased actin polymerization and protrusion of a pseudopod in the direction of a gradient. (jove.com)
  • dictyBase curators, in collaboration with Jeff Williams from the University of Dundee, have developed an ontology to describe Dictyostelium, the Dictyostelium anatomy ontology , where we define the structural makeup of Dictyostelium and its composing parts including the different cell types, throughout its life cycle. (dictybase.org)
  • Familiarity with one or more of the following would be advantageous: Dictyostelium, yeast, protein purification, actin or tubulin biochemistry, microscopy. (bio.net)
  • A ribosomal protein gene cluster is encoded in the mitochondrial DNA of Dictyostelium discoideum: UGA termination codons and similarity of gene order to Acanthamoeba castellanii. (nih.gov)
  • Here, using a tractable model system, Dictyostelium discoideum , we show that diacylglycerol kinase activity might contribute to the cellular mechanism of action of the epilepsy and bipolar disorder treatment, valproic acid. (biologists.org)
  • Glycogen synthase kinase 3 (GSK3) is a central regulator of metazoan development and the Dictyostelium GSK3 homologue, GskA, also controls cellular differentiation. (biologists.org)
  • These observations show that GskA functions throughout Dictyostelium development, to regulate several key aspects of cellular patterning. (biologists.org)
  • The intrinsic features of Dictyostelium and a set of well-established molecular tools enabled the authors to prove that Dictyostelium is a representative cellular model for Legionella infection. (asmscience.org)
  • Dictyostelium transformants that overproduce the extracellular form of cyclic nucleotide phosphodiesterase and so accumulate a reduced amount of cAMP are blocked in development after aggregation in the form of a tight mound, prior to formation of the apical tip. (pnas.org)
  • Dictyostelium cells are generally thought to move under buffer using actin-driven pseudopods, but a single report also describes them as using small, transient blebs as well as pseudopods ( Yoshida and Soldati, 2006 ), and uniform stimulation with cyclic-AMP can induce blebbing, suggesting that blebs are under chemotactic control ( Langridge and Kay, 2006 ). (rupress.org)
  • Dictyostelium Transcriptional Activators and a short description of their functions. (highveld.com)
  • The research areas presented in this chapter include the analysis of the transcriptional host cell response to Legionella infection and the use of custom tailored Dictyostelium mutant cells to identify determinants of susceptibility and resistance. (asmscience.org)
  • When we compared Dictyostelium to other sequenced organisms, we found it had more homology to animals than to either plants or fungi,' says Angelika A. Noegel, who led the sequencing at the University of Cologne, Germany. (genomenewsnetwork.org)
  • We propose that mechanical resistance induces bleb-driven movement in Dictyostelium , which is chemotactic and controlled through PI3-kinase. (rupress.org)
  • The paper we have published ( Gaudet, Williams, Fey and Chisholm, 2008 ) ( full text from BioMed Central ) gives a description of the ontology and definitions of the terms, in the context of the life cycle of Dictyostelium. (dictybase.org)
  • We review here two commonly used methods for transformation of Dictyostelium cells: calcium phosphate precipitation and electroporation. (dictybase.org)
  • Sutoh, K. (1993) A transformation vector for Dictyostelium discoideum with a new selectable marker bsr. (scirp.org)
  • Grow Dictyostelium cells in HL5 medium at 21 to 23°C (20) to a density of 1-2x 10 6 cells /ml. (dictybase.org)
  • We show that Dictyostelium cells switch from using predominantly pseudopods to blebs when migrating under agarose overlays of increasing stiffness. (rupress.org)
  • Yoshida, M., Ishida, S. and Iizuka, Y. (1991) Mutants of Dictyostelium discoideum with altered carbohydrate moieties of contact site A. Cell Structure Function, 16, 383-390. (scirp.org)
  • First, Dictyostelium microarrays were used to study the systemic host response to Legionella infection. (asmscience.org)