Loading...
  • genome
  • Comparative genome sequence analysis of three cultured representatives suggests that cellular requirements for nitrogen are largely provided by regenerated ammonium and organic compounds (polyamines, allophanate, and urea), while typical sources of carbon include amino acids, glyoxylate, and aromatic metabolites. (asm.org)
  • citrate
  • Moreover, preliminary RNA-Seq analysis of an itaconic acid producing AB1.13 CAD strain has led to the identification of the putative cytosolic citrate synthase citB which was induced in an IA producing strain. (biomedcentral.com)
  • Expression
  • We have previously shown that expression of cadA from A. terreus results in itaconic acid production in A. niger AB1.13, albeit at low levels. (biomedcentral.com)
  • Baker's yeast has a single heterodimeric peroxisomal ABC transporter, Pxa1p/Pxa2p ( Figure 1 A), required for utilization of fatty acids [ 7 , 8 ] and the pxa1/2Δ mutant has proved to be a useful tool for expression and analysis of ABCD proteins from other organisms [ 9 - 12 ]. (biochemsoctrans.org)
  • Proteins
  • We discuss a speculative alternating access model for ABCD proteins based on the mammalian ABC transporter associated with antigen processing (TAP) and compare it to the novel mechanism suggested by the recent PglK crystal structures and biochemical data. (biochemsoctrans.org)
  • organic
  • The industrially relevant filamentous fungus Aspergillus niger is widely used in industry for its secretion capabilities of enzymes and organic acids. (biomedcentral.com)
  • Biotechnologically produced organic acids promise to be an attractive alternative for the chemical industry to replace petrochemicals. (biomedcentral.com)
  • Bio-based organic acid production promises to be an attractive alternative for the chemicals industry to replace petrochemicals and for the food industry as an food additive. (biomedcentral.com)
  • More recently, filamentous fungi have stepped forward as potent producers of organic acids and the industrial producers of enzymes. (biomedcentral.com)
  • pathway
  • Import of β-oxidation substrates into peroxisomes is mediated by ATP binding cassette (ABC) transporters belonging to subfamily D. In order to enter the β-oxidation pathway, fatty acids are activated by conversion to fatty acyl-CoA esters, a reaction which is catalysed by acyl-CoA synthetases (ACSs). (biochemsoctrans.org)
  • A distinctive function of peroxisomes is the β-oxidation pathway in which fatty acids (FA) are degraded with the concomitant generation of acetyl-CoA [ 1 ]. (biochemsoctrans.org)
  • production
  • This low-level production is boosted fivefold by the overexpression of mttA and mfsA in itaconic acid producing AB1.13 CAD background strains. (biomedcentral.com)
  • Controlled batch cultivations with AB1.13 CAD + MFS + MTT strains showed increased production of itaconic acid compared with AB1.13 CAD strain. (biomedcentral.com)
  • We have overexpressed citB in a AB1.13 CAD + MFS + MTT strain and by doing so hypothesize to have targeted itaconic acid production to the cytosolic compartment. (biomedcentral.com)
  • 3-Hydroxypropionic acid (3-HP) is an important platform chemical, serving as a precursor for a wide range of industrial applications such as the production of acrylic acid and 1,3-propanediol. (biomedcentral.com)
  • Remove
  • Eric Gouaux studies the molecular mechanisms of communication between nerve cells by studying the receptors and transporters that detect and remove neurotransmitters from synapses, the sites of nerve cell communication. (hhmi.org)