AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation ScienceHealth Care
AntimetabolitesTetrahydrofolate DehydrogenaseAntimetabolites, AntineoplasticGene Expression ProfilingGene ExpressionFolic Acid AntagonistsTetrahydrouridineFloxuridineMethotrexateTrabeculectomyGene Expression RegulationInosine MonophosphateDrug ResistancePhosphoribosyl PyrophosphateTrimethoprim ResistanceFluorouracilGene AmplificationMitomycinCell LineLeukemia L12106-MercaptopurinePyrimethamineRNA, MessengerCricetinaeOligonucleotide Array Sequence AnalysisCytarabineTranscription, GeneticBase SequenceDihydropteroate SynthasePurinesCricetulusPromoter Regions, GeneticAntineoplastic AgentsMolecular Sequence DataGenesDrug SynergismSulfadoxineTumor Cells, CulturedGene Expression Regulation, DevelopmentalDNACell SurvivalCHO CellsDrug Screening Assays, AntitumorDeoxycytidinePyrimidine DimersCell CycleDrug Evaluation, PreclinicalNucleic Acid HybridizationAntimalarialsReverse Transcriptase Polymerase Chain ReactionGene Expression Regulation, NeoplasticCell DivisionNeoplasmsTranscription FactorsPolymerase Chain ReactionGene Expression Regulation, EnzymologicPlasmodium falciparumMutationGene Expression Regulation, PlantKineticsGene Expression Regulation, BacterialOvaryRetinoblastoma-Binding Protein 1Drug CombinationsCloning, MolecularGene Expression Regulation, ViralMalaria, FalciparumDNA-Binding ProteinsBlotting, SouthernCells, CulturedUltraviolet RaysSignal TransductionRestriction MappingCluster AnalysisDNA RepairTranscriptomeGene Expression Regulation, FungalDNA Restriction EnzymesGenes, ReporterTransfectionDNA PrimersBlotting, NorthernMicroarray AnalysisUp-RegulationDNA DamageRNACell DifferentiationAmino Acid SequenceCell Line, TumorTranscriptional ActivationNuclear ProteinsDown-RegulationDNA, ComplementaryIn Situ HybridizationTrans-ActivatorsGene Regulatory NetworksRepressor ProteinsBinding SitesTime FactorsPlasmids
Inhibits dihydrofolate reductaseInhibitorMultipleCellDihydrofolate reductaseMethotrexateTumorChemotherapyMRNA expressionInhibitionPathwaysCells
Inhibits dihydrofolate reductase2
  • Methotrexate inhibits dihydrofolate reductase (DHFR), causing a block in the reduction of dihydrofolate to tetrahydrofolate. (medscape.com)
  • Pralatrexate is a folate analog metabolic inhibitor that competitively inhibits dihydrofolate reductase (DHFR) selectively in cancer cells overexpressing the reduced folate carrier protein-1 (RFC-1). (drugbank.com)
Inhibitor1
  • WP1130 (Degrasyn) is a novel selective small molecular deubiquitinase inhibitor and a Bcr/Abl destruction pathway activator that specifically and rapidly down-regulates both wild-type and mutant Bcr/Abl protein without affecting bcr/abl gene expression in chronic myelogenous leukemia (CML) cells. (adooq.com)
Multiple1
  • Ethyl 2,4,6-trihydroxybenzoate, a natural product isolated and purified from the herbs of Celtis biondii, suppresses cellular cholesterol accumulation in a dose-dependent manner and induces the transcriptional activation of LXR-α/-β-responsive genes, and is a dual-LXR modulator that regulates the expression of key genes in cholesterol homeostasis in multiple cells without inducing lipid accumulation in HepG2 cells. (csnpharm.com)
Cell1
  • 13 As well, the study dose far exceeded the target dose for patients with peripheral T-cell lymphoma and pralatrexate does not inhibit the human ether-a-go-go-related gene (hERG) K + channel 13 . (drugbank.com)
Dihydrofolate reductase3
  • Methotrexate in pediatric osteosarcoma: response and toxicity in relation to genetic polymorphisms and dihydrofolate reductase and reduced folate carrier 1 expression. (cdc.gov)
  • Background: Methotrexate (MTX) is an inhibitor of dihydrofolate reductase (DHFR), an enzyme involved in nucleotide synthesis, and is widely used in therapy, but its effi cacy is often compromised by the development of resistance in cancer cells. (medscimonit.com)
  • Methotrexate inhibits dihydrofolate reductase (DHFR), causing a block in the reduction of dihydrofolate to tetrahydrofolate. (medscape.com)
Methotrexate2
Tumor1
  • The growth of normal and cancerous cells is genetically controlled by the balance or imbalance of oncogene and tumor suppressor gene protein products. (basicmedicalkey.com)
Chemotherapy2
  • ATIC Gene Polymorphism and Histologic Response to Chemotherapy in Pediatric Osteosarcoma. (cdc.gov)
  • To identify potential targets as modulators in MTX chemotherapy, we determined gene expression profi les upon MTX treatment. (medscimonit.com)
MRNA expression1
  • FOXM1 mRNA expression was quantitated using real-time PCR. (bvsalud.org)
Inhibition2
  • The reviewed research data implies that these co-therapies exhibited a synergistic effect on various cancer cells, where apigenin sensitized the chemo drug through different pathways including a significant reduction in overexpressed genes, AKT phosphorylation, NFκB, inhibition of Nrf2, overexpression of caspases, up-regulation of p53 and MAPK, compared to the monotherapies. (biomedcentral.com)
  • It has been shown to induce an anti-inflammatory effect, most likely by inhibition of COX-2 expression and via IL-6 signaling. (adooq.com)
Pathways1
  • Muramyl dipeptide directly enhances osteoblast differentiation by up-regulating Runx2 gene expression through MAPK pathways. (csnpharm.cn)
Cells5
  • Material/Methods: Expression profi les in human erythroleukemia K562 cells were determined after short treatment with MTX or once resistance to MTX was established. (medscimonit.com)
  • Results: Differentially expressed genes were selected considering a factor of 2 with respect to their expression in control cells. (medscimonit.com)
  • Eight genes were found to be overexpressed and 14 underexpressed both in MTX-treated and resistant cells. (medscimonit.com)
  • Conclusions: Expression of IMPDH2 is increased in K562 cells upon short treatment with MTX and once resistance to MTX is established. (medscimonit.com)
  • FOXM1 and TS expression were increased in the 5-FU treated cells but were suppressed in the SioA treated cells. (bvsalud.org)