Mammalian embryoBlastocystVitroOocytesEmbryogenesisDrosophilaPost-implantationMorulaDifferencesTrophectodermEmbryonicPluripotentVivoFertilization1991ZygoticTranscriptionalGametesPaternalDevelopmentChromosomeMorphologicalDynamicsTime-lapseLineagesPolarityChromatinMouseMethylationEpiblastHumanCellsCellFrameworkProcessSystemPregnancyData
Mammalian embryo3
- By the proper period of its implantation in to the maternal uterus, the mammalian embryo includes three specific cell types. (synanet2020.com)
- These findings can be integrated in a new unified framework that regards the early mammalian embryo as a self-organizing system. (nature.com)
- Haaf T., The battle of the sexes after fertilization: behaviour of paternal and maternal chromosomes in the early mammalian embryo. (biologie-journal.org)
Blastocyst9
- In mouse, a unidirectional demethylation process from the zygote stage to blastocyst stage is observed using either reduced representation bisulfite sequencing (RRBS) or single-base resolution whole-genome bisulfite sequencing (WGBS) method. (nature.com)
- Human embryonic stem cell-derived blastocyst-like spheroids resemble human trophectoderm during early implantation process To study the use of human embryonic stem cell-derived trophoblastic spheroids (BAP-EB). (embies.com)
- At blastocyst stage, the segregation of the three primordial lineages is accompanied by establishment of differential patterns of DNA methylation and post-translational modifications of histones, such as H3K27me3. (sciencegate.app)
- To facilitate a quantitative morphological analysis of early mouse development under controlled conditions, a perfusion culture system capable of supporting embryogenesis to blastocyst stage has been developed. (sciencegate.app)
- 2- to 4-cell and morula- to blastocyst-stage mouse embryos were cultured for 1 h in tritiated leucine at two specific activities and their subsequent development followed in vitro and in vivo (after transfer to recipients), respectively. (sciencegate.app)
- We after that review latest theoretical techniques that formalize the systems underlying destiny decisions in the internal cell mass from the blastocyst stage embryo. (synanet2020.com)
- We conclude by recommending that cell-to-cell conversation provides a system to exploit and buffer inter-cellular variability inside a self-organized procedure that culminates in Cyclosporin C the reproducible development from the adult mammalian blastocyst stage embryo that's prepared for implantation in to the maternal uterus. (synanet2020.com)
- Origin from the three cell types composed of the mammalian blastocyst Through the first couple of days of advancement the mouse embryo undergoes some specific morphological and mobile events to changeover from an individual totipotent cell, the zygote, to a ~200 cell embryo composed of three specific, spatially organized cell types at around embryonic day time (E) 4.5 (discover Fig. 1 for a synopsis of preimplantation advancement and staging strategies). (synanet2020.com)
- The space inside the embryo spreads, and the morula becomes a blastocyst. (nature.com)
Vitro7
- 1989). In Vitro Developmental Toxicity of Five Direct-Acting Alkylating Agents in Rodent Embryos: Structure-Activity Patterns. (wikipedia.org)
- Moreover, the dynamic of H3K27me3 at PCH during in vitro conversion from naïve to primed pluripotent state and during ESCs derivation suggests that the mechanisms underlying the control of this histone mark at PCH are different in embryo and in vitro. (sciencegate.app)
- Currently, the ability to culture in vitro totipotent cells possessing molecular and functional features like those of an early embryo in vivo has been a challenge. (sciencegate.app)
- This study underscores the subtle differences between in vitro derived TBLCs and in vivo mouse early developmental cell stages at the single-cell transcriptomic level. (sciencegate.app)
- Huang J.C., Lei Z.L., Shi L.H., Miao Y.L., Yang J.W., Ouyang Y.C., Sun Q.Y., Chen D.Y., Comparison of histone modifications in in vivo and in vitro fertilization mouse embryos. (biologie-journal.org)
- Since the first in vitro fertilization-embryo transfer (IVF-ET) baby was born in 1978, IVF-ET procedures have made remarkable progress, and various assisted reproductive technologies have been implemented. (ecerm.org)
- Poor in vitro embryo development is a major obstacle in porcine assisted reproduction. (mdpi.com)
Oocytes2
- Bovine NALP5, NALP8, and NALP9 Genes: Assignment to a QTL Region and the Expression in Adult Tissues, Oocytes, and Preimplantation Embryos. (fbn-dummerstorf.de)
- The morphokinetics of embryo development was found to vary between IVF- and ICSI-fertilized oocytes, at least until the 6-cell stage. (ecerm.org)
Embryogenesis4
- However, robust and large-scale genome-wide reprogramming of DNA methylome occurs during two critical developmental processes: (1) development of primordial germ cells and (2) pre-implantation embryogenesis. (nature.com)
- Although the genome-wide DNA demethylation is believed to be a hallmark of mammalian embryogenesis, previous study also indicated that the somatic form of dnmt1 ( dnmt1s ) is actually expressed at each stage of pre-implantation embryos and plays a role in the maintenance of DNA imprinting 8 . (nature.com)
- These results strongly suggest the possibility of DNA remethylation during pre-implantation embryogenesis. (nature.com)
- Using QCANet, we were able to extract several quantitative criteria of embryogenesis from 11 early mouse embryos. (nature.com)
Drosophila3
- Driever, W. & Nüsslein-Volhard, C. A gradient of bicoid protein in Drosophila embryos. (nature.com)
- Driever, W. & Nüsslein-Volhard, C. The bicoid protein determines position in the Drosophila embryo in a concentration-dependent manner. (nature.com)
- Drosophila embryos are easily amenable to imaging because they are more transparent than the embryos of other model organisms, such as mice. (nature.com)
Post-implantation2
- Exposure to NMU during pre-implantation, post-implantation, organogenesis, or by paternal exposure can result in these effects. (wikipedia.org)
- These long-term effects can be traced to pre- and post-implantation development, and in both cases, the effects are usually sex specific. (embies.com)
Morula2
- Primarily, the zygote undergoes successive rounds of cell department (known as cleavages), with the 8-cell stage, cells small and polarize to create NR4A3 the morula (Johnson & Ziomek, 1981). (synanet2020.com)
- At the 8-cell stage, the embryo becomes compact, and the cells form a spherical mass called a morula. (nature.com)
Differences4
- Our comparative embryology approach uncovered intriguing differences as well as similarities in a fundamental developmental process among mammals, reinforcing the importance of cross-species investigations. (bvsalud.org)
- We conclude by discussing differences and commonalities in preimplantation advancement between different mammalian varieties. (synanet2020.com)
- We showed that the extracted criteria could be used to evaluate the differences between individual embryos. (nature.com)
- However, these differences did not affect the clinical outcomes of the embryo. (ecerm.org)
Trophectoderm1
- We have shown that the establishment of cell polarity via aPKC is a conserved event in the initiation of the trophectoderm (TE) placental programme in mouse, cow and human embryos. (bvsalud.org)
Embryonic8
- Furthermore, due to ethical and legal concerns, very limited techniques can be applied to human embryos to validate some of significant conclusions drawn from descriptive studies regarding human embryonic development. (nature.com)
- Developmental plasticity enables the appearance of long-term effects in offspring caused by exposure to environmental stressors during embryonic and foetal life. (embies.com)
- The developmental potential within pluripotent cells in the canonical model is restricted to embryonic tissues, whereas totipotent cells can differentiate into both embryonic and extraembryonic tissues. (sciencegate.app)
- The TBLCs exhibited totipotency transcriptionally and acquired expanded developmental potential with the ability to yield various embryonic and extraembryonic tissues that may be employed as novel mouse developmental cell models. (sciencegate.app)
- To address this question, single-cell RNA sequencing was applied to TBLCs and cells from early mouse embryonic developmental stages and the data were integrated using canonical correlation analyses. (sciencegate.app)
- Differential expression analyses were performed between TBLCs and multi-embryonic cell stages to identify differentially expressed genes. (sciencegate.app)
- Remarkably, a subpopulation within the TBLCs population expressed a high level of the totipotent-related genes Zscan4s and displayed transcriptomic features similar to mouse two-cell stage embryonic cells. (sciencegate.app)
- Observation of embryos in a time-lapse incubator can provide useful information about embryonic developmental events if images are automatically captured [ 7 8 9 10 ]. (ecerm.org)
Pluripotent1
- Fulka H., St John J.C., Fulka J., Hozak P., Chromatin in early mammalian embryos: achieving the pluripotent state. (biologie-journal.org)
Vivo2
- Intrafallopian transfer of gametes and early stage embryos for in vivo culture in cattle. (fbn-dummerstorf.de)
- However, it is disputed whether TBLCs are 'true' totipotent stem cells equivalent to in vivo two-cell stage embryos. (sciencegate.app)
Fertilization1
- Adenot P.G., Szollosi M.S., Geze M., Renard J.P., Debey P., Dynamics of paternal chromatin changes in live one-cell mouse embryo after natural fertilization. (biologie-journal.org)
19911
- 1991). Induction of Fetal Malformations After Treatment of Mouse Embryos with Methylnitrosourea at the Preimplantation Stages. (wikipedia.org)
Zygotic1
- Bellier S., Chastant S., Adenot P., Vincent M., Renard J.P., Bensaude O., Nuclear translocation , and carboxyl-terminal domain phosphorylation of RNA polymerase II delineate the two phases of zygotic gene activation in mammalian embryos. (biologie-journal.org)
Transcriptional4
- During preimplantation development, male and female embryos exhibit an extensive transcriptional dimorphism mainly driven by incomplete X chromosome inactivation. (embies.com)
- Genetic and pharmacological tests have offered insights in to the transcriptional and signaling systems managing lineage decisions in the Cyclosporin C preimplantation embryo. (synanet2020.com)
- Adenot P.G., Mercier Y., Renard J.P., Thompson E.M., Differential H4 acetylation of paternal, maternal chromatin precedes DNA replication, and differential transcriptional activity in pronuclei of 1-cell mouse embryos. (biologie-journal.org)
- Aoki F., Worrad D.M., Schultz R.M., Regulation of transcriptional activity during the first , and second cell cycles in the preimplantation mouse embryo. (biologie-journal.org)
Gametes1
- Here we report genome-wide composition, patterning, and stage-specific dynamics of DNA methylation in pre-implantation rhesus monkey embryos as well as male and female gametes studied using an optimized tagmentation-based whole-genome bisulfite sequencing method. (nature.com)
Paternal1
- Howlett S.K., Reik W., Methylation levels of maternal , and paternal genomes during preimplantation development. (biologie-journal.org)
Development7
- Unlike in rodents, X chromosome inactivation is not observed during monkey pre-implantation development. (nature.com)
- These early developmental stages are crucial for the establishment of epigenetic marks that will be conserved throughout development, making it a particularly susceptible period for the appearance of long-term epigenetic-based phenotypes. (embies.com)
- Through the definition of a coordinate system based on mesh structure and the development of a special sectioning procedure, sections can be localized within the intact embryo and three-dimensional coordinates given to any element of embryo volume. (sciencegate.app)
- The mechanisms underlying the appearance of asymmetry between cells in the early embryo and consequently the specification of distinct cell lineages during mammalian development remain elusive. (nature.com)
- Graham, C. F. & Lehtonen, E. Formation and consequences of cell patterns in preimplantation mouse development. (nature.com)
- Braude P., Bolton V., Moore S., Human gene expression first occurs between the four- , and eight-cell stages of preimplantation development. (biologie-journal.org)
- Thus, embryo development is highly dynamic. (nature.com)
Chromosome1
- A cage of stable microtubule loops drives the late stages of chromosome clustering. (bvsalud.org)
Morphological1
- Embryo selection based on a morphological assessment at a few points in time has several limitations for single ET. (ecerm.org)
Dynamics1
- However, the timing and localisation of molecular markers differ across species, with rat embryos more closely recapitulating human and cow developmental dynamics, compared with the mouse. (bvsalud.org)
Time-lapse5
- For time-lapse observation of early-stage D rosophila embryos, Keller et al. (nature.com)
- A total of 1,830 normal fertilized embryos were obtained from 272 IVF and ICSI cycles that underwent ovum retrieval culture using a time-lapse system (Embryoscope) from June 2013 to March 2015. (ecerm.org)
- All embryos were investigated by a detailed time-lapse analysis that measured the developmental events in the hours after IVF or ICSI insemination. (ecerm.org)
- Several methods have been proposed for culturing embryos using a time-lapse incubator to maintain a stable environment. (ecerm.org)
- 11 ] developed the first time-lapse system for studying morphokinetics in human embryos. (ecerm.org)
Lineages1
- The next cell fate decision bifurcates the ICM in to the Epi and PrE lineages. (synanet2020.com)
Polarity1
- However, the mechanisms transducing cell polarity into cell fate in cow and human embryos are unknown. (bvsalud.org)
Chromatin1
- Giraldo A.M., Hylan D.A., Ballard C.B., Purpera M.N., Vaught T.D., Lynn J.W., Godke R.A., Bondioli K.R., Effect of epigenetic modifications of donor somatic cells on the subsequent chromatin remodeling of cloned bovine embryos. (biologie-journal.org)
Mouse7
- Throughout this review we concentrate on the decision between your Epi as well as the PrE destiny, and focus on the mouse as the utmost studied magic size program for preimplantation advancement extensively. (synanet2020.com)
- Figure 1: Classic models for lineage segregation in the early mouse embryo. (nature.com)
- Louvet-Vallee, S., Vinot, S. & Maro, B. Mitotic spindles and cleavage planes are oriented randomly in the two-cell mouse embryo. (nature.com)
- Rossant, J. & Vijh, K. M. Ability of outside cells from preimplantation mouse embryos to form inner cell mass derivatives. (nature.com)
- Piotrowska, K. & Zernicka-Goetz, M. Role for sperm in spatial patterning of the early mouse embryo. (nature.com)
- Bouniol C., Nguyen E., Debey P., Endogenous transcription occurs at the 1-cell stage in the mouse embryo. (biologie-journal.org)
- We showed that QCANet can be applied not only to developing mouse embryos but also to developing embryos of two other model species. (nature.com)
Methylation1
- By the 8-cell stage, remethylation becomes more pronounced than demethylation, resulting in an increase in global DNA methylation. (nature.com)
Epiblast1
- We show that this histone modification is first enriched at PCH in the whole embryo and evolves into a diffuse distribution in epiblast during its specification and maturation. (sciencegate.app)
Human3
- In contexts with limited single-cell samples, such as the early human embryo inference of transcription factor-gene regulatory network (GRN) interactions is especially difficult. (bvsalud.org)
- Here, we assessed application of different linear or non-linear GRN predictions to single-cell simulated and human embryo transcriptome datasets. (bvsalud.org)
- MICA predicted co-localisation of the AP-1 transcription factor subunit proto-oncogene JUND and the TFAP2C transcription factor AP-2γ in early human embryos. (bvsalud.org)
Cells2
- Because the cells in embryos are considerably crowded, an algorithm to segment individual cells in detail and accurately is needed. (nature.com)
- In early embryos, cells are loosely connected to each other. (nature.com)
Cell6
- Promoters of genes associated with oxidative phosphorylation are preferentially remethylated at the 8-cell stage, suggesting that this mode of energy metabolism may not be favored. (nature.com)
- In 1-cell and 2-cell embryos Dnmt1s is derived from the oocyte, whereas from the 2-cell stage onward the embryo starts to synthesize its own Dnmt1s 8 . (nature.com)
- Quantitative evaluation of tissue-processing procedures has permitted selection of conditions which reduce changes in linear dimensions to −1·6 ± 1·8 % in two-cell embryos. (sciencegate.app)
- advancement in minimal moderate, the preimplantation embryo is a tractable system for analysis and manipulation in the single-cell level highly. (synanet2020.com)
- Dealing with this relevant query needs calculating cell-to-cell variability in the embryo, understanding its source, and identifying its practical relevance for following destiny decisions. (synanet2020.com)
- Kopecny V., Fléchon J.E., Camous S., Fulka J. Jr., Nucleologenesis , and the onset of transcription in the eight-cell bovine embryo: fine-structural autoradiographic study. (biologie-journal.org)
Framework1
- We talk about the meaning of the results in the framework of developmental mobile potential as well as the hereditary control of destiny decisions in this technique. (synanet2020.com)
Process1
- Therefore, embryo selection is a very important process before ET. (ecerm.org)
System1
- The use of a mesh system allows identification of individual embryos by position, and control of their orientation during culture and preparation for light and electron microscopy. (sciencegate.app)
Pregnancy1
- thus, most IVF centers induce a higher pregnancy rate by implanting more than one embryo. (ecerm.org)
Data1
- Images of the embryo provide more accurate data to guide embryo selection [ 5 6 ]. (ecerm.org)