Developmental physiology in vitro oocyte maturation techniques. Medical search. Frequent questions

This study investigated the effect of the flavonoid-based compound isorhamnetin (ISO) on maturation and developmental competence in oxidative stress-exposed porcine oocytes in vitro. (bvsalud.org)
In conclusion, melatonin could be a promising tool for improving sperm competence for fertilizing oocytes and embryo development in sheep. (vetmedmosul.com)
Currently, its estimation is predominantly based on morphological analysis, which is subjective and does not consistently reflect the developmental competence of the oocytes. (biomedcentral.com)
however, it is not per se sufficient to assure oocyte developmental competence [ 13 ]. (biomedcentral.com)

1998. Influence of organochlorine pesticides on maturation and post-fertilization development of bovine oocytes in vitro . (cdc.gov)
Increased IGFBP-4 and -5 protease along with lower amounts of IGFBP-4 binding activity and greater amounts of free IGF-I are some of the earliest developmental changes documented in bovine growing antral follicles. (bioone.org)
The paper is a very comprehensive and well-illustrated micro-anatomical and ultrastructural developmental study on the altering layout of the bovine forestomachs in the 26-80 days old embryo. (wava-amav.org)
Melatonin supplementation has been shown to promote bovine and porcine oocytes' maturation rate and increase their blastocyst of IVF embryos (16). (vetmedmosul.com)
It could be concluded that the presence of urea at 40 mg/dL in maturation medium reduced the percentage of bovine oocyte maturation in vitro. (sciencegate.app)
ABSTRACT We aimed to assess the effects of melatonin in the in vitro production of bovine embryos. (sciencegate.app)
Our experiment was further divided into two: the first was to assess the effect of different concentrations of melatonin (treatments) on the maturation rate of COCs, and the second was to assess the effects of melatonin treatments on the in vitro production of bovine embryos. (sciencegate.app)

At puberty the number of oocytes decreases even more to reach about 60,000 to 80,000 per ovary, and only about 500 mature oocytes will be produced during a woman's life, the others will undergo atresia(degeneration). (wikipedia.org)
To prove the ability of mature oocytes to develop into embryos, 900 oocytes were processed for fertilization in vitro. (sciencegate.app)
Conclusion: FSH supplementation significantly improves oocyte maturation and yields mature oocytes for future embryo development in vitro. (sciencegate.app)

Relating in vitro to in vivo exposures with physiologically-based tissue dosimetry and tissue response models. (cdc.gov)
This study was conducted to evaluate in vivo and in vitro development of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection. (bioone.org)
In vivo development was assessed after transfer of injected oocytes to the oviducts of recipient mares. (bioone.org)
Hamel JF, Mercier A (2007) In vivo investigation of oocyte transit and maturation in a broadcast-spawning holothurian. (isbweb.org)
This review focuses on the differential responses of cell types to ATZ toxicity, testicular effects of ATZ in both in vitro and in vivo models and chemopreventive strategies, so as to highlight the current state of the art on the toxicological outcomes of ATZ exposure in several experimental model systems. (frontiersin.org)
The synchronization and ovulatory responses of Sangsari cross bred ewes and metabolism of energy substrates in 8-cell stage embryos to hatched blastocysts stage produced in vitro or in vivo were investigated. (biomedcentral.com)
While the pattern of oxidation was similar among in vitro and in vivo derived embryos, a low pyruvate to lactate ratio was the preferred substrate of embryos derived in vitro . (biomedcentral.com)
The production of CO2 from glucose by sheep embryos has been determined[ 3 ] and results have indicated that little glucose is utilized by the embryos of sheep and that blastocysts derived from oocytes, matured and fertilized in vitro , oxidized glucose at a lower rate than those developed in vivo . (biomedcentral.com)

These results demonstrate that 2 ISO improves the quality of porcine oocytes by protecting them against oxidative stress during IVM and enhances subsequent embryo development in vitro. (bvsalud.org)

Oogenesis consists of several sub-processes: oocytogenesis, ootidogenesis, and finally maturation to form an ovum (oogenesis proper). (wikipedia.org)
Second Polar Body (Discarded afterward) + Ovum Oocyte meiosis, important to all animal life cycles yet unlike all other instances of animal cell division, occurs completely without the aid of spindle-coordinating centrosomes. (wikipedia.org)
Diagram showing the reduction in number of the chromosomes in the process of maturation of the ovum . (wikipedia.org)
With ovulation the secondary oocyte (or ovum) is expelled from the follicle on the ovary surface. (pediagenosis.com)

When the inability of male transgenic rats to transmit the transgene is not associated with mosaicism, microinsemination techniques such as intracytoplasmic sperm injection (ICSI) and round spermatid injection (ROSI) can be used for passage of the transgenes. (go.jp)

These microinsemination techniques can be combined with cryopreservation of sperm and spermatid cells, leading to successful transmission of transgenes carried in male gametes. (go.jp)
The results of various articles have shown that freezing of oocytes and the cryopreservation method are effective for the expression of miRNAs and, in some cases, cause changes in the expression of miRNAs and epigenetic changes in the resulting embryo. (bvsalud.org)
This literature review study aimed to investigate the effects of oocyte cryopreservation in both pre-maturation and post-maturation stages, the cryopreservation method and the type of cryoprotectants (CPA) used on the expression of some epigenetic-related genes and miRNAs. (bvsalud.org)

Oogonia enter meiosis during embryonic development, becoming oocytes. (wikipedia.org)
Understanding the biological functions of miRNAs in frozen maturated oocytes may provide a better understanding of embryonic development and a comparison of fertility conservation in female mammals. (bvsalud.org)
The aim of this study was to determine the in vitro maturation rate of Bligon goat oocytes supplemented with follicle-stimulating hormone (FSH), and their ability for further embryonic development after in vitro fertilization. (sciencegate.app)

Further, melatonin and its receptors play a significant role in antioxidant defense mechanism of ovary for follicular growth and maturation. (jrhm.org)
The aim of this study is to investigate the role of activated ovarian endothelial cells in early in-vitro follicular development. (biomedcentral.com)
Beginning on day 4 of culture, we observed significantly higher follicular and oocyte growth rates in follicles co-cultured with AOECs compared with follicles on gel or matrigel. (biomedcentral.com)
This model is a novel platform for the in vitro maturation of early follicles and for the future exploration of endothelial-follicular communication. (biomedcentral.com)
Duplicating the complex interaction of growth factors, cellular and hormonal signals required for follicular development and oocyte maturation in vitro is challenging. (biomedcentral.com)
Oocytes, sperms, and embryos are shielded from oxidative stress in their natural environment by free radical scavengers found in oviductal and follicular fluids. (vetmedmosul.com)

Using integrative physiology to improve the sustainability of fish aquaculture. (exeter.ac.uk)
Male European seabass, already predominant (~ 70%) in cultured stocks, show a high incidence (20-30%) of precocious sexual maturation under current aquaculture practices, leading to important economic losses for the industry. (biomedcentral.com)
In seabass aquaculture, the high incidence (20 to 30%) of precocious sexual maturation in males during the first year of life places a critical restraint to production parameters. (biomedcentral.com)

It is developed from the primary oocyte by maturation. (wikipedia.org)
The succeeding phase of ootidogenesis occurs when the primary oocyte develops into an ootid. (wikipedia.org)

ROS are free radicals that are continuously produced by oxidative metabolic reactions and increase in vitro conditions, causing aging of both the oocyte (7) and sperm (8), as well as perhaps impairing fertilization and embryo development. (vetmedmosul.com)

As follicles grow, they acquire receptors for luteinizing hormone, a pituitary hormone that reinitiates meiosis in the oocyte and causes ovulation of a fertilizable egg. (wikipedia.org)
These protease-induced decreases in IGFBP-4 and -5 likely cause increased levels of bioavailable (or free) IGFs that stimulate steroidogenesis and mitogenesis in developing dominant follicles, which ultimately prepare the follicle(s) and oocyte(s) for successful ovulation and fertilization. (bioone.org)
Synchronization of estrus followed by induction of ovulation are basic techniques in all embryo transfer programs. (biomedcentral.com)

Oocytes were collected from slaughterhouse-derived ovaries, matured in vitro, and injected with frozen-thawed stallion sperm. (bioone.org)

Maintenance of meiotic arrest also depends on the presence of a multilayered complex of cells, known as a follicle, that surrounds the oocyte. (wikipedia.org)
Removal of the oocyte from the follicle causes meiosis to progress in the oocyte. (wikipedia.org)
The oocyte is surrounded by cumulus cells (also termed the corona radiata) from the follicle and spermatozoa must break through this outer layer to reach the oocyte itself (Figure 10.3). (pediagenosis.com)
Cumulus oocyte complexes (COCs) of cows derived from follicle aspiration were divided into three groups. (sciencegate.app)

Treatment with 2 µM ISO (2 ISO) increases the developmental rate of oxidative stress-exposed porcine oocytes during in vitro maturation (IVM). (bvsalud.org)

Next, 600 oocytes were then stained with 1% aceto-orcein to examine maturation based on changes in the configuration of chromosomes and nuclear membrane breakdown. (sciencegate.app)

Our ICSI procedure includes the use of a piezo-driven small-sized injection pipette (2-4 μm in diameter), isolated sperm heads, and freshly ovulated oocytes. (go.jp)
In addition, other corrective measures include sperm separation for assisted reproductive techniques. (hindawi.com)
The acrosomal cap of the head of the sperm breaks down, releasing enzymes that dissolve the zona pellucida locally allowing the spermatozoon to enter the oocyte (Figure 10.4). (pediagenosis.com)
oxidative stress is the most prominent one, which will cause damage or alter the genetic material of the sperm and reduce the quality of the oocytes, and has a crucial impact on the possibility of developing embryos even after implantation. (vetmedmosul.com)
For this purpose, we have incubated ram sperm using four different treatments in terms of antioxidants: melatonin, cysteamine, vitamin C, and vitamin E. They were incubated by the standard methods of maturation and capacitation of sperm. (vetmedmosul.com)
In the last decade, in vitro fertilization and sperm capacitation techniques have significantly advanced. (vetmedmosul.com)
To fertilize the oocytes, sperm must undergo a series of physiological and biochemical alterations in vitro (3). (vetmedmosul.com)
Oxidative stress is one of the key factors contributing to a decrease in sperm quality during the maturation and capacitation of the sperm (5). (vetmedmosul.com)
thereby, oocytes and sperm are highly vulnerable to reactive oxygen species (ROS) attacks, affecting their quality and viability (6). (vetmedmosul.com)
Dr Upasna Sharma and colleagues in the Department of Biochemistry and Molecular Pharmacology at the University of Massachusetts Chan Medical School, USA, report intriguing findings regarding the influence of paternal diet on the constituents of sperm during the maturation process. (scientia.global)
After leaving the testis, sperm complete their maturation whilst travelling through the epididymis, a long, convoluted, tubular organ in which the concentration of tRFs increases between the entry point (caput) and exit point (cauda). (scientia.global)
This prompted Dr Sharma and the team to speculate that epididymosomes transfer tRFs into the sperm during the maturation process. (scientia.global)

The two cells that result from this division are the definitive oocyte and the second polar body. (pediagenosis.com)
The second polar body receives little cytoplasm, allowing the definitive oocyte to maintain its size. (pediagenosis.com)
Aceto-orcein staining was conducted to evaluate the maturation of oocytes based on the achievement of metaphase II phase that is indicated by the presence of metaphase plate and/or first polar body. (sciencegate.app)

Mammalian oocytes are maintained in meiotic prophase arrest for a very long time-months in mice, years in humans. (wikipedia.org)
However, as the oocyte grows, these proteins are synthesized, and meiotic arrest becomes dependent on cyclic AMP. (wikipedia.org)
In the oocyte, cyclic GMP prevents the breakdown of cyclic AMP by the phosphodiesterase PDE3, and thus maintains meiotic arrest. (wikipedia.org)

In June 2017 a group of experts in anthropology, biology, kinesiology, neuroscience, physiology, and psychology convened in Canterbury, UK, to address questions relating to the placebo effect in sport and exercise. (canterbury.ac.uk)

Several physiological parameters, such as viability, mitochondrial activity, and reactive oxygen species (ROS) levels have been also reported as useful markers of oocyte quality in marine invertebrates. (biomedcentral.com)

One thousand five hundred cumulus-oocyte complexes were matured for 24 h in tissue culture medium 199 supplemented with 50 IU/L FSH or without FSH (control). (sciencegate.app)
First, matured oocytes were evaluated its morphology based on the expansion of cumulus cells and PB1 extrusion. (sciencegate.app)
The cumulus-oocyte complexes (COCs) were distributed among treatments at concentrations of 0, 10-1, 10-3 and 10-5 µMol/L melatonin. (sciencegate.app)

1974. Pesticidal chemicals affecting some energy-linked functions of rat liver mitochondria in vitro . (cdc.gov)
In the oocyte, mitochondria play a central role in cellular metabolism providing energy for successful cytoplasm and nucleus maturation, fertilization, embryo development, calcium homeostasis, and apoptosis initiation. (biomedcentral.com)

The secondary oocyte was paused partway through meiosis II (see Chapter 9). (pediagenosis.com)

The blastocyst stage of oocyte developed embryos was reached by 12.43±2.15% and 22.28±4.86% of the control and treatment groups, respectively. (sciencegate.app)

Extensive research has unraveled a niche for melatonin that is of great significance for the female reproductive physiology. (jrhm.org)
Usage of melatonin during in vitro fertilization (IVF) procedures has been found to improve oocyte quality, survival, and fecundity. (jrhm.org)
Therefore, in future, melatonin can be implicated as preferable therapeutic especially in IVF and assisted reproductive techniques. (jrhm.org)
The oocytes were fertilized by spermatozoa that had been capacitated with two groups of fertilization media, the first group containing melatonin and the second group containing cysteamine. (vetmedmosul.com)

This has been shown for intrauterine insemination (IUI), in vitro fertilization (IVF), and ICSI, and increased incidences of chromosomal abnormalities, minor and major birth defects, or early childhood cancers, particularly in the male offspring, have been linked to these procedures [ 8 - 16 ]. (hindawi.com)

Results: The results indicated that FSH supplementation significantly increased oocyte maturation rate (65.21±7.26 vs. 43.25±6.23%) as indicated by extrusion of PB1 and homologous chromosome pairing and lined in the equator. (sciencegate.app)

Because the granulosa cells and oocyte are connected by gap junctions, cyclic GMP also decreases in the oocyte, causing meiosis to resume. (wikipedia.org)
With the fusion of the spermatozoon cell membrane the oocyte is triggered to continue meiosis. (pediagenosis.com)
Oocytes are highly specialized cells that undergo the unique processes of meiosis and fertilization and execute a molecular program for development [ 1 ]. (biomedcentral.com)

Among those, several candidate genes have been shown to control the early patterning and/or the late synaptic maturation of specific neuronal subpopulations controlling the balance between excitation and inhibition in the developing cortex and cerebellum. (neurotransmitter.net)
Here we used two-electrode voltage clamp electrophysiology to compare the actions of CBD with those of the major central endocannabinoid, 2-arachidonoyl glycerol (2-AG) on human recombinant GABAA receptors (synaptic α1-6βg2 and extrasynaptic α4β2δ) expressed on Xenopus oocytes. (researchgate.net)

Toward a developmental neurobiology of autism. (neurotransmitter.net)
In the present article, we review our current understanding of the developmental mechanisms patterning the balance between excitation and inhibition in the context of the neurobiology of autism. (neurotransmitter.net)

This review describes the available information regarding the role of integrins in human reproductive physiology and discusses their clinical implications. (go.jp)

The expression of miRNAs at the right time and place is crucial for the oocyte's maturation and the embryo's subsequent development. (bvsalud.org)

A growing body of evidence suggests that parental environment can affect the metabolic health and physiology of their offspring in a phenomenon known as the intergenerational inheritance of acquired traits, or epigenetic inheritance, although the mechanisms by which this occurs are poorly understood. (scientia.global)

Throughout a female's reproductive lifespan, the ovaries undergo continual structural changes that are crucial for the maturation of ovarian follicles and the production of sex steroid hormones. (mdpi.com)

American Journal of Physiology - Endocrinology and Metabolism. (edu.au)
Surgical embryo recovery was performed on Days 4 and 6 after onset of estrus (Day 0) and recovered embryos were subjected to comparative metabolism studies with in vitro derived embryos at the same stage of development. (biomedcentral.com)
They began by determining whether paternal diet could indeed influence the metabolism in offspring generated by in vitro fertilisation. (scientia.global)

The granulosa cells produce a small molecule, cyclic GMP, that diffuses into the oocyte through the gap junctions. (wikipedia.org)

Embryologically we talk about developmental processes occurring a number of days after fertilisation. (pediagenosis.com)

Materials and Methods: Experiment was conducted at the Laboratory of Animal Physiology and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, using Bligon goat ovaries obtained from local slaughterhouse around Yogyakarta. (sciencegate.app)
Despite several recent studies investigating molecular markers related to oocyte quality, methods currently available for their determination pose various technical challenges and limitations. (biomedcentral.com)

Many investigations have been designed to gain new insights into the mechanisms underlying oocyte quality based on a differential analysis applied to molecular, biochemical, transcriptomic, or proteomic data. (biomedcentral.com)
Nevertheless, the complexity of the techniques applied to assess gene expression and protein profile is a major bottleneck for the use of molecular markers in the oocyte quality assessment [ 12 ]. (biomedcentral.com)

In vitro development of early follicles necessitates a complex interplay of growth factors and signals required for development. (biomedcentral.com)
These drawbacks could be overcome by the ability to mature early follicles in vitro. (biomedcentral.com)

Addressing those 'big questions' of high societal relevance have the merit of creating high visibility and also funding opportunities, however, classic comparative physiology and biochemistry is running the risk of becoming marginalized in this process, if the underlying physiological and biochemical processes cannot be visualized accordingly. (easychair.org)

MicroRNAs (miRNAs) are small non-encoding RNAs that actively regulate biological and physiological processes, and play an important role in regulating gene expression in all cells, especially in most animal cells, including oocytes and embryos. (bvsalud.org)

These findings are linked to melatonin's ability to reduce ROS and its adverse effects on oocytes, such as aging, apoptosis, and its capacity to bind to specific DNA sequences and interact with cytosolic molecules to regulate antioxidant genes (17). (vetmedmosul.com)

Oogenesis starts with the process of developing primary oocytes, which occurs via the transformation of oogonia into primary oocytes, a process called oocytogenesis. (wikipedia.org)

It is commonly believed that, when oocytogenesis is complete, no additional primary oocytes are created, in contrast to the male process of spermatogenesis, where gametocytes are continuously created. (wikipedia.org)

Moreover, the in vitro experiment demonstrated high glucose amounts' deleterious effect on embryo development [ 3 ]. (ejast.org)

The paper "Formation of Genetically Mosaic Mouse Embryos and Early Development of Lethal (t12/t12)-Normal Mosaics," by Beatrice Mintz, describes a technique to fuse two mouse embryos into a single embryo. (asu.edu)
Influence of organochlorine pesticides on development of mouse embryos in vitro . (cdc.gov)
Further knowledge of the integrin-mediated regulation of cell growth and cell survival may facilitate our understanding of many key aspects of development and physiology in reproduction. (go.jp)
With the development of new techniques for genomic evaluation of preimplantation embryos, it has been possible to better understand the effects of ART. (bvsalud.org)
In view of the known modulation of reproductive development by swimming exercise in other teleost species, we aimed at investigating the effects of sustained swimming on reproductive development in seabass males during the first year of life in order to determine if swimming could potentially reduce precocious sexual maturation. (biomedcentral.com)
Scientists attempting to discover the basic requirements for successful embryo development have a hurdle when using in vitro embryo manufacturing strategies (2). (vetmedmosul.com)

A common feature shared by all juvenile vertebrates is the quiescent state of activity of the brain-pituitary-gonad (BPG) axis that characterizes the post-embryonic developmental period until the onset of puberty. (biomedcentral.com)

This paper was applauded for using state-of-the-art imaging and computational morphometric analysis of the pre- and postnatal developmental changes in the shape and composition of the murine femur, providing new insights in the ossification and growth patterns of developing bone. (wava-amav.org)

In vitro maturation allows female goat (does) contributes toward reproduction despite the fact that the animal has been slaughtered. (sciencegate.app)

Two publications have challenged the belief that a finite number of oocytes are set around the time of birth. (wikipedia.org)
Over the past few decades, our understanding of how our adult health can be shaped by events occurring before birth has developed into a well-supported concept, the Developmental Origins of Health and Disease (DOHaD). (mdpi.com)

Quality control in human in vitro fertilization. (shengsci.com)
The implementation of suitable quality control (QC) is not only required for the accreditation of a human in vitro fertilization (IVF) laboratory, but is also fundamental to its success. (shengsci.com)
In this study, we reported boric acid's protective effects on the quality of nonylphenol (NP)-exposed oocytes. (bvsalud.org)
Oocyte quality has been defined as the ability of oocytes to be fertilized and produce viable progeny. (biomedcentral.com)
Hence, to date, an effective and simple proxy of oocyte quality parameters has not been established yet making difficult for an accurate assessment of the female gamete quality before fertilization. (biomedcentral.com)

This study aimed to determine the influence of antioxidants on the achievement of In vitro culture (IVC) and sperm's ability to adhere to and penetrate further into In vitro maturated oocytes. (vetmedmosul.com)

Anatomy2

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes2

Disciplines and Occupations1

Competence4

Bovine7

Mature oocytes3

Vivo8

Embryo development in vitro1

Ovum4

Intracytoplasmic Sperm Inj1

Cryopreservation3

Embryonic development3

Follicular6

Aquaculture3

Primary oocyte2

Fertilization and embryo development1

Ovulation3

Frozen-thawed1

Follicle4

Porcine1

Chromosomes1

Sperm12

Polar Body3

Meiotic3

Biology1

Viability1

Cumulus3

Mitochondria2

Secondary1

Blastocyst1

Melatonin4

ICSI1

Significantly1

Meiosis3

Synaptic2

Neurobiology2

Reproductive physiology1

Oocyte's1

Metabolic1

Undergo1

Metabolism3

Granulosa1

Fertilisation1

Methods2

Molecular2

Follicles2

Biochemical1

Physiological1

Apoptosis1

Oogenesis1

Oocytogenesis1

Glucose1

Development6

Puberty1

Patterns1

Reproduction1

Birth2

Quality5

Achievement1