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Ambystoma mexicanumRegenerationExtremitiesTurbellariaNotophthalmusAmbystomaNotophthalmus viridescensForelimbSalamandridaeGene Expression Regulation, DevelopmentalAnimal FinsPlanariansCell DifferentiationTriturusSignal TransductionZebrafishTranscription FactorsHomeodomain ProteinsTailIn Situ HybridizationMolecular Sequence DataPhenotypeGene Expression ProfilingCell LineageAmputationMutationReceptors, NotchWnt ProteinsBody PatterningHedgehog ProteinsMorphogenesisGene Expression Regulation, PlantEmbryo, NonmammalianAmino Acid SequenceUrodelaHornsDrosophila ProteinsBase SequencePleurodelesWilms TumorModels, BiologicalPolymorphism, Single NucleotideNerve TissueOligonucleotide Array Sequence AnalysisSoftware DesignGene Expression RegulationPersonality DevelopmentArabidopsisLarvaMesodermReverse Transcriptase Polymerase Chain ReactionGenotypeGene ExpressionFlowersDNA-Binding ProteinsArabidopsis ProteinsAnimals, Genetically ModifiedPhylogenyBiological EvolutionCyclic AMP Response Element-Binding Protein AMetabolic Networks and PathwaysThymus GlandBiosynthetic PathwaysCell ProliferationAdolescent DevelopmentNational Institutes of Health (U.S.)DrosophilaStem CellsGenes, PlantGenes, InsectRNA, MessengerSequence Analysis, DNARepressor ProteinsAnaplasiaCloning, MolecularDrosophila melanogasterSequence Homology, Amino AcidImmunohistochemistrySequence AlignmentZebrafish ProteinsAmputation StumpsDNA, ComplementaryBasic Helix-Loop-Helix Transcription FactorsCaenorhabditis elegansTrans-ActivatorsAllantoinTime FactorsCell DedifferentiationAnimal StructuresPentosan Sulfuric PolyesterPlant ProteinsLymphopoiesisMice, Inbred C57BLDNA PrimersWound HealingCaenorhabditis elegans ProteinsCells, CulturedGenetic Predisposition to DiseaseNuclear ProteinsInsect Proteins
RegenerativeProliferationBiologyRegenerateOntologyDifferentiationAmputationNodalCellularLimbTissuesStagesEmbryonic stemProcessesWoundProteinsHeat shockMutationsRegeneration requiresLineagesZebrafishAxolotlOrganGeneticInhibitionExpressionAnalysesRepresentsDifferentiateOrgansGrowthStructuresSpecificStressForm
Regenerative8
  • Many of the genes that are involved in the original development of tissues are reinitialized during the regenerative process. (wikipedia.org)
  • Using Affymetrix Gene Chip analysis, we have identified genes linked to regenerative success downstream of BMP signalling, including the BMP inhibitor Gremlin and the stress protein Hsp60 ( no blastema in zebrafish). (biomedcentral.com)
  • Extrapolating this to mammals it may be the case that there is more regenerative ability in mammals than has typically been thought to exist and that inducing regeneration in humans may not be as impossible as it may seem. (ehu.eus)
  • The regenerative abilities of mammals is described and it turns out that there are several examples of classical epimorphic regeneration involving a blastema as exemplified by the regenerating Urodele limb that can be seen in mammals. (ehu.eus)
  • therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells. (biomedcentral.com)
  • Consequently, because of the increased rate of RF in terminal stage and few alternative treatments, new investigations focused on regenerative medicine have been developed, including the use of stem cells (SCs) [ 7 ]. (biomedcentral.com)
  • Neural control of growth and size in the axolotl limb regenerate [2] "Upon the completion of the developmental stages of regeneration, when the regenerative organ known as the blastema completes patterning and differentiation, the limb regenerate is proportionally small in size. (edu.au)
  • Members of the free-living genus Planaria are widely interrogated in the realm of stem cell biology due to their remarkable regenerative abilities [ 8 ]. (biomedcentral.com)
Proliferation6
  • In recent years, interest in the possible molecular regulators of cell proliferation and differentiation in a wide range of regeneration models has grown significantly, but the cell kinetics of this process remain largely a mystery. (mdpi.com)
  • Amputation-induced proliferation occurred predominantly within the epidermal and intestinal epithelium, as well as wound-adjacent muscle fibers, where clusters of cells at the same stage of the cell cycle were found. (mdpi.com)
  • At its most elementary level, regeneration is mediated by the molecular processes of gene regulation and involves the cellular processes of cell proliferation, morphogenesis and cell differentiation. (wikipedia.org)
  • Epimorphic regeneration can be thought of as occurring in two phases: wound healing and cell proliferation. (biomedcentral.com)
  • In both processes, rapid proliferation gives rise to new tissue, cell fate has to be specified within that tissue, and distinct positional identities have to be established to generate a properly patterned structure. (frontiersin.org)
  • Inhibition of apoptosis did not affect branchial sac stem cell proliferation but prevented the survival of progenitor cells. (biologists.com)
Biology2
  • In biology, regeneration is the process of renewal, restoration, and tissue growth that makes genomes, cells, organisms, and ecosystems resilient to natural fluctuations or events that cause disturbance or damage. (wikipedia.org)
  • Join us and publish in a specialized journal in Developmental Biology, university-based, with a long and prestigious history. (ehu.eus)
Regenerate5
  • After the limb or tail has been autotomized, cells move into action and the tissues will regenerate. (wikipedia.org)
  • Neural cells, for example, express growth-associated proteins, such as GAP-43, tubulin, actin, an array of novel neuropeptides, and cytokines that induce a cellular physiological response to regenerate from the damage. (wikipedia.org)
  • Xenopus tadpoles can regenerate their tails, limb buds and the lens of the eye, although the ability of the latter two organs to regenerate diminishes with advancing developmental stage. (biomedcentral.com)
  • N1 transgenic hindlimbs, which do not regenerate, do not form an apical epithelial cap or cone shaped blastema following amputation. (biomedcentral.com)
  • In addition, a non-regenerating species has been induced to regenerate by altering only one signaling pathway. (ehu.eus)
Ontology2
Differentiation2
  • In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. (biomedcentral.com)
  • Moreover, FTIR spectroscopy resulted in a non-invasive, rapid and precise technic that together with principal component analysis allows to characterize chemical and structurally both kind of cells and also discriminate and determine different stages along the cell differentiation process. (biomedcentral.com)
Amputation3
  • Loss or amputation of the axolotl limb leads to the regeneration of the lost limb from trunk tissue, thereby repeating a developmental sequence as a repair process. (edu.au)
  • After amputation, granular hemocytes infiltrate the blastema of regenerating cephalic tentacles of the freshwater snail Pomacea canaliculata. (unimore.it)
  • Histological studies focusing on the early cephalic tentacle regeneration in P. canaliculata, have demonstrated that wound closure and blastema formation took place within 24 h post amputation (hpa). (unimore.it)
Nodal1
  • However, the opposing pathway to Nodal signaling during LR axis establishment is not clear. (echinobase.org)
Cellular2
  • mitotically active cells of intact segments do not significantly contribute to the blastemal cellular sources. (mdpi.com)
  • This study provides a new paradigm for SARS-CoV-2 cellular entry through binding of ApoE on the lipoviral particles to host cell receptor(s). (bvsalud.org)
Limb2
Tissues6
  • Strategies include the rearrangement of pre-existing tissue, the use of adult somatic stem cells and the dedifferentiation and/or transdifferentiation of cells, and more than one mode can operate in different tissues of the same animal. (wikipedia.org)
  • 873 During the developmental process, genes are activated that serve to modify the properties of cell as they differentiate into different tissues. (wikipedia.org)
  • Dedifferentiation of cells means that they lose their tissue-specific characteristics as tissues remodel during the regeneration process. (wikipedia.org)
  • Once the wound is healed, the cells of the stump must mobilise under the wound epidermis and begin the process of replacing lost tissues, by forming a proliferating blastema. (biomedcentral.com)
  • Furthermore, we detected strong membrane LDLR expression on SARS-CoV-2 Spike-positive cells in human lung tissues, whereas no or low ACE2 expression was detected. (bvsalud.org)
  • Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)
Stages5
  • Expression patterns of germline marker genes at the LG (J and K) and EPL (L-N) stages. (echinobase.org)
  • The complete integumentary organ system (IOS) regeneration and scarless wound healing mechanism, which occurs in specific species, body sites and developmental stages, clearly shows that mechanical stress signals and immune responses play important roles in determining the wound healing mode. (mdpi.com)
  • Therefore, the expres sion of each gene was measured by RT PCR during dif ferent stages of S. flexneri growth in Luria Bertani at pH 7. (gp120-inhibitors.com)
  • Regarding vibrational spectroscopy analysis by FTIR, bands related with biomolecules were shown on mPSCs and DKCs spectra, observing distinct differences between cell lineages and maturation stages. (biomedcentral.com)
  • The sequence of axolotl embryonic developmental stages was characterised in the late 1980's. (edu.au)
Embryonic stem1
Processes2
  • These genes, acquired at conception or mutated during life, make the affected person prone to most cancers by altering or impairing several processes: & Production of nuclear transcription factors that control cell growth. (ehd.org)
  • This Special Issue intends to provide examples of current exciting work on RA signaling, with a special focus on developmental processes in the embryo, highlighting intriguing results and exciting perspectives for the future of RA research. (mdpi.com)
Wound3
  • We have used this line to show that BMP function is not only required for appendage regeneration but that it is specifically needed to generate a proliferating blastema while being dispensable for wound healing [ 12 ]. (biomedcentral.com)
  • Advances in tissue engineering technology have led to the production of novel human skin equivalents and organoids that reproduce cell-cell interactions with tissue-scale tensional homeostasis, and enable us to evaluate skin tissue morphology, functionality, drug response and wound healing. (mdpi.com)
  • After bisection across the mid-body, apoptosis occurred only in the regenerating basal fragments, although both fragments contained a part of the branchial sac, suggesting that apoptosis is unilateral at the wound site and the presence of branchial sac stem cells is insufficient for regeneration. (biologists.com)
Proteins1
  • ADPKD is due to mutations in the genes PKD1 and PKD2, which encode polycystin proteins. (medscape.com)
Heat shock1
  • We have developed a heat shock inducible transgenic line ( N1 ) of Xenopus in which the BMP antagonist Noggin can be induced at a specific time during either development or regeneration, repressing BMP signalling [ 10 , 12 ]. (biomedcentral.com)
Mutations4
  • Mutations in these genes can be inherited in autosomal dominant or recessive forms, with varying levels of penetrance. (medscape.com)
  • ARPKD is due to mutations in PKHD1, a large gene that encodes fibrocystin/polyductin, which plays critical roles in collecting-tubule and biliary development. (medscape.com)
  • TS is caused by mutations in the suppressor genes TSC1 and TSC2, which encode hamartin and tuberin, respectively. (medscape.com)
  • VHLS is due to mutations in the VHL gene, which increases the risk for malignancy, including RCC. (medscape.com)
Regeneration requires1
  • A pharmacological screen and siRNA-mediated gene knockdown indicated that regeneration requires canonical Wnt signaling. (biologists.com)
Lineages1
  • Embryonic or adult SCs by definition are characterized by their self-renewal and potentiality, peculiarities that allow them to give rise to more SCs and differentiate into various cell lineages under appropriate conditions. (biomedcentral.com)
Zebrafish1
Axolotl1
  • With the recent completion of the axolotl genome assembly and established methods to manipulate gene functions, the axolotl is poised to provide new insights about paedomorphosis and the role of thyroid hormone in development and evolution. (edu.au)
Organ3
  • Muted multi-organ transcriptional reprogramming and metabolism rewiring suggest that a KD initiates and mitigates systemic changes induced by the virus. (bvsalud.org)
  • The kidney is a complex organ composed of several cell types, and it is conformed approximately by 11,000 nephrons. (biomedcentral.com)
  • Regeneration involves the activity of adult stem cells in the branchial sac, which proliferate and produce migratory progenitor cells for tissue and organ replacement. (biologists.com)
Genetic1
  • Pattern formation in the morphogenesis of an animal is regulated by genetic induction factors that put cells to work after damage has occurred. (wikipedia.org)
Inhibition1
  • We find that BMP signaling is required for proper expression of various patterning genes and that its inhibition causes major defects in the regenerated limbs. (edu.au)
Expression9
  • In the current study, we have taken advantage of the N1 transgenic line to directly compare morphology and gene expression in same stage regenerating vs. BMP signalling deficient non-regenerating hindlimb buds. (biomedcentral.com)
  • Comparison of gene expression in stage matched N1 vs. wild type hindlimb buds has revealed several new targets for regeneration research. (biomedcentral.com)
  • By perturbing BMP activities, we demonstrated that BMP signaling is required for activating the expression of the left-sided genes and the formation of the left-sided structures. (echinobase.org)
  • Expression patterns of bmp genes and pSmad staining during sea urchin development. (echinobase.org)
  • BMP is required for left-sided structure formation and gene expression in the aboral veg2 descendants.ISH of bmp2/4 and pSmad immunostaining in control and DM-treated embryos at the LG (A, treated from 24 to 48 hpf) or EPL stage (B, treated from 42 to 48 hpf). (echinobase.org)
  • The arrows in (H) indicate right-sided gene expression. (echinobase.org)
  • Conclusions In this work we have shown that the expression of gluQ rs, a gene codifying an enzyme involved in the formation of GluQ present on the tRNAAsp, is under the control of http://www.selleckchem.com/products/mek162.html the dksA promoter. (gp120-inhibitors.com)
  • We showed previously that cytoplasmic release of mtDNA activates the cGAS STING TBK1 pathway resulting in interferon-stimulated gene (ISG) expression that promotes antiviral immunity4. (regenerativemedicine.net)
  • Here, we find that persistent mtDNA stress is not associated with basally activated NF-κB signalling or interferon gene expression typical of an acute antiviral response. (regenerativemedicine.net)
Analyses1
  • F-H) ISH analyses of LR marker genes in BMP signaling-perturbed embryos. (echinobase.org)
Represents1
  • The plasmid containing a fragment from the dksA promoters to the beginning of the gluQ rs gene, with the first five amino acids of GluQ RS, named pVCPDT, represents the full length dksA gene with its native promoters. (gp120-inhibitors.com)
Differentiate2
  • This should not be confused with the transdifferentiation of cells which is when they lose their tissue-specific characteristics during the regeneration process, and then re-differentiate to a different kind of cell. (wikipedia.org)
  • ESCs are obtained from inner cell mass, characterized by unlimited self-renewal and pluripotentiality, which allow them to differentiate into various specialized cells types in a morphologically and functionally way, characteristics that make them attractive for developing differentiated kidney cells (DKCs) [ 9 ]. (biomedcentral.com)
Organs1
  • Once wounded, their cells become activated and restore the organs back to their pre-existing state. (wikipedia.org)
Growth2
  • It is well-established that early embryonic signaling pathways are critical for growth and patterning of new tissue during regeneration. (frontiersin.org)
  • Branchial sac-derived stem cells also replenish recycling cells lining the pharyngeal fissures during homeostatic growth. (biologists.com)
Structures1
  • Thousands of copies of the circular mtDNA are present in most cell types that are packaged by TFAM into higher-order structures called nucleoids1. (regenerativemedicine.net)
Specific2
  • The cDNA was amplified using specific PCR primers for each gene of interest. (gp120-inhibitors.com)
  • After the injection of clodronate liposomes, a specific computer-assisted image analysis protocol still evidenced the presence of granular hemocytes in the tentacle blastema. (unimore.it)
Stress1
  • In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)
Form1
  • Furthermore, the underlying mesenchyme remains rounded and does not expand to form a cone shaped blastema, a normal feature of successful regeneration. (biomedcentral.com)