• This study investigated the effect of the flavonoid-based compound isorhamnetin (ISO) on maturation and developmental competence in oxidative stress-exposed porcine oocytes in vitro. (bvsalud.org)
  • Treatment with 2 µM ISO (2 ISO) increases the developmental rate of oxidative stress-exposed porcine oocytes during in vitro maturation (IVM). (bvsalud.org)
  • These results demonstrate that 2 ISO improves the quality of porcine oocytes by protecting them against oxidative stress during IVM and enhances subsequent embryo development in vitro. (bvsalud.org)
  • Poor in vitro embryo development is a major obstacle in porcine assisted reproduction. (mdpi.com)
  • Although assisted reproductive techniques (ART) have helped to solve many infertility problems, they cause changes in the expression of miRNA and genes in oocytes and preimplantation embryos, and the effect of these changes on the future of offspring is unknown, and has caused concerns. (bvsalud.org)
  • With the development of new techniques for genomic evaluation of preimplantation embryos, it has been possible to better understand the effects of ART. (bvsalud.org)
  • The low maturation rate of oocytes is an important reason for female infertility and failure of assisted pregnancy. (cambridge.org)
  • This study may be an important supplement for the mechanism of oocyte meiotic resumption and provide new diagnostic targets and treatment clues for infertility patients with oocyte maturation disorder. (cambridge.org)
  • The process involves sucking out the nucleus of a somatic (body) cell and injecting it into an oocyte that has had its nucleus removed Using an approach based on the protocol outlined by Tachibana et al. (wikipedia.org)
  • When ZP3 is knocked down, lamin A will be partially transferred to the nucleus of oocytes. (cambridge.org)
  • Brunetti D., Lagutina I., Perota A., Colleoni S., Duchi R., Lucchini F., Lazzari G., Galli C. "Derivation of bovine fetal fibroblasts harboring OCT4-GFP vector and analysis of GFP expression in cloned embryos. (istitutospallanzani.it)
  • Brunetti D., Lagutina I., Perota A., Colleoni S., Lucchini F., Lazzari G., Galli C. "Screening of RFP expression and reactivation of oct4-promoter in horse cloned embryos. (istitutospallanzani.it)
  • MicroRNAs (miRNAs) are small non-encoding RNAs that actively regulate biological and physiological processes, and play an important role in regulating gene expression in all cells, especially in most animal cells, including oocytes and embryos. (bvsalud.org)
  • They form characteristic cell clusters in suspension culture that express a set of genes associated with pluripotency and can differentiate into endodermal, ectodermal and mesodermal cells both in vitro and in vivo. (wikipedia.org)
  • This literature review study aimed to investigate the effects of oocyte cryopreservation in both pre-maturation and post-maturation stages, the cryopreservation method and the type of cryoprotectants (CPA) used on the expression of some epigenetic-related genes and miRNAs. (bvsalud.org)
  • Previous studies have revealed the miR164 family and the miR164-targeted NAC transcription factor genes in rice ( Oryza sativa ) and Arabidopsis that play versatile roles in developmental processes and stress responses. (chinaagrisci.com)
  • In the current study, we utilized modified Spirulina maxima pectin nanoparticles as a supplement to improve porcine in vitro maturation medium. (mdpi.com)
  • They showed that opposing gradients of bone morphogenetic protein (BMP) and Nodal, two transforming growth factor family members that act as morphogens, are sufficient to induce molecular and cellular mechanisms required to organize, in vivo or in vitro, uncommitted cells of the zebrafish blastula animal pole into a well-developed embryo. (wikipedia.org)
  • Using in vitro and cellular techniques, we present a mVP40 assembly model highlighting two distinct oligomerization interfaces: the (N-terminal domain (NTD) and C-terminal domain (CTD)) in mVP40. (uci.edu)
  • The expression of miRNAs at the right time and place is crucial for the oocyte's maturation and the embryo's subsequent development. (bvsalud.org)
  • Understanding the biological functions of miRNAs in frozen maturated oocytes may provide a better understanding of embryonic development and a comparison of fertility conservation in female mammals. (bvsalud.org)
  • In our previous studies, we found that zona pellucida 3 (ZP3) was strongly concentrated in the nuclear region of germinal vesicle (GV) oocytes and interacted with aryl hydrocarbon receptor-interacting protein-like 1 (AIPL1) and lamin A to promote GVBD. (cambridge.org)
  • The results of various articles have shown that freezing of oocytes and the cryopreservation method are effective for the expression of miRNAs and, in some cases, cause changes in the expression of miRNAs and epigenetic changes in the resulting embryo. (bvsalud.org)
  • One example is the transformation of iris cells to lens cells in the process of maturation and transformation of retinal pigment epithelium cells into the neural retina during regeneration in adult newt eyes. (wikipedia.org)
  • The fact that transdetermination (change of the path of differentiation) often occurs for a group of cells rather than single cells shows that it is induced rather than part of maturation. (wikipedia.org)
  • A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
  • Embryos were prepared after in vitro maturation either by parthenogenetic activation or somatic cell nuclear transfer (SCNT) technique. (bvsalud.org)
  • It also discusses the effects of ooplasmic transfer on oocytes and embryos, and mtDNA. (fertiligy.com)
  • Ooplasm donation is a technique where donor oocytes are used to make embryos. (fertiligy.com)
  • The developmental potential of early embryos is mainly dictated by the quality of the oocyte. (elifesciences.org)
  • The direct transfer methods of frozen-thawed, in vivo- derived, and in vitro- produced (IVF) bovine embryos using 1.5 M ethylene glycol (EG) with or without sucrose (SUC) are used widely under on-farm conditions, not only in Japan but also globally. (go.jp)
  • The pregnancy rate with direct transfer of frozen-thawed bovine embryos in either EG or GLY-SUC has been found to not differ from conventional freezing with GLY and traditional dilution techniques. (go.jp)
  • Pregnancy rates following direct transfer of frozen-thawed bovine embryos were affected by the developmental stage of the embryos and the parity of the recipients. (go.jp)
  • We performed mitochondrial DNA Next Generation Sequencing and determined that one mtDNA variant harbored by EPCs was present in oocytes, preimplantation embryos and somatic tissues over three generations in our mini-pig model indicating the potential germline origin of EPCs. (oncotarget.com)
  • The third approach comprises a variety of proposals for engineering "biological artifacts" possessing some of the developmental capacities of natural embryogenesis (but lacking the organismal character of human embryos) and containing cells from which pluripotent stem cell lines can be derived. (georgetown.edu)
  • They showed that opposing gradients of bone morphogenetic protein (BMP) and Nodal, two transforming growth factor family members that act as morphogens, are sufficient to induce molecular and cellular mechanisms required to organize, in vivo or in vitro, uncommitted cells of the zebrafish blastula animal pole into a well-developed embryo. (wikipedia.org)
  • Our proof-of-concept experiments show that replacement of the entire cytoplasm of oocytes from a sensitive mouse strain overcomes massive embryo developmental arrest characteristic of non-manipulated oocytes. (elifesciences.org)
  • Embryo transfer entails many procedures and techniques, of which embryo freezing is an important component in bovine embryo transfer. (go.jp)
  • Embryo freezing techniques have been developed over the last 40 years, allowing practical availability, and have become essential for cattle reproduction management under field conditions. (go.jp)
  • In vitro embryo production. (embrapa.br)
  • The ultrasound determination of ovarian response in embryo donor goats still needs to be proven, especially because the non-invasive technique is mandatory when the embryo collection is done by transcervical route. (embrapa.br)
  • During ovulation, a newly fertilized oocyte carries mtDNA from both parents. (fertiligy.com)
  • The oocyte has two mtDNA haploplasmy states - mitochondrial heteroplasmy in the donor, and mitochondrial homoplasmy in the recipient oocyte. (fertiligy.com)
  • This pre-clinical model demonstrates the high efficiency and potential of the MST technique, not only to prevent the transmission of mtDNA mutations, but also as a new potential treatment for patients with certain forms of infertility refractory to current clinical strategies. (elifesciences.org)
  • The quality of mitochondria and the numbers of copies of its genome, mitochondrial DNA (mtDNA), in oocytes are beginning to be considered, along with other factors, as indicators of oocyte quality, especially in the context of aging [ 5 - 11 ]. (oncotarget.com)
  • The process involves sucking out the nucleus of a somatic (body) cell and injecting it into an oocyte that has had its nucleus removed Using an approach based on the protocol outlined by Tachibana et al. (wikipedia.org)
  • Recently, by interrogating the single-cell transcriptome of human FGCs and gonadal somatic cells, we found that human FGC development is asynchronous in later developmental stages. (nature.com)
  • Fetal germ cells (FGCs) are embryonic precursors of mature gametes, i.e. sperm or oocytes. (nature.com)
  • PACRG is a protein found in the mitochondrial sheath of the oocyte prior to fertilization and at the tail of the sperm after exposure to oocyte extract. (fertiligy.com)
  • Male-factor infertility can be overcome by a technique called intracytoplasmic sperm injection (ICSI), which directly injects a sperm into an oocyte, and is one of the most widely used treatments in the fertility clinic [ 3 ]. (oncotarget.com)
  • In this study, we aimed to isolate EVs from the porcine oviductal epithelial cells (POECs) that were primed with steroid hormones including estradiol (E2) and progesterone (P4), mimicking the in vivo conditions of the reproductive cycle and studied their effects on in vitro produced embryonic development. (bvsalud.org)
  • Actin networks are responsible for driving ooplasm segregation in zebrafish oocytes. (fertiligy.com)
  • Epigenomic reprogramming of FGCs is important for resetting the developmental potential, erasing epigenetic memory, and establishing unipotency in human germ cells. (nature.com)
  • To address these questions, we cultured 7-week male FGCs from testis in vitro with or without its antagonist LDN-193189 2HCl (LDN), and analyzed the transcriptional and epigenetic differences at single-cell level between these two conditions. (nature.com)
  • Because ooplasms are genetically diverse, the oocytes can impose epigenetic modifications on the parental genomes. (fertiligy.com)
  • Medical procedures known as in vitro fertilization (IVF) help many individuals experiencing infertility to have children. (elifesciences.org)
  • On the other hand, treatment for female-factor infertility is often restricted to in vitro fertilization, since ICSI does not appear to be an effective treatment for poor quality oocytes [ 4 ]. (oncotarget.com)
  • Female-factor infertility can be caused by poor oocyte quality and depleted ovarian reserves. (oncotarget.com)
  • The aim of this study was to investigate the effect of different progestagens during ovarian stimulation on follicular population and oocyte morphological quality in Santa Inês ewes. (embrapa.br)
  • In conclusion, the source of progestagens used during the ovarian stimulation protocol does not affect the follicular population, nor the oocyte quality. (embrapa.br)
  • They form characteristic cell clusters in suspension culture that express a set of genes associated with pluripotency and can differentiate into endodermal, ectodermal and mesodermal cells both in vitro and in vivo. (wikipedia.org)
  • Here, we explore the utility of the maternal spindle transfer (MST) technique as a reproductive approach to enhance oocyte developmental competence. (elifesciences.org)
  • An increasing number of women are delaying childbirth, and, since oocyte quality declines dramatically after 35 years of age [ 1 , 2 ], more women are requiring assisted reproductive treatments. (oncotarget.com)
  • Similarly, the oocytes' actin networks generate pulling forces near the ooplasm-yellow granule interface. (fertiligy.com)
  • Using this technique, it is possible to generate human egg cells for IVF that have the genetic material from the intended mother without the defects in the cytoplasm that may be responsible for infertility. (elifesciences.org)
  • Through bioinformatic analysis, we identified several potential target genes of the miRNAs present in royal jelly, including those involved in developmental processes and cell differentiation. (bvsalud.org)
  • Ooplasm controls many processes during the oocyte's maturation and activation. (fertiligy.com)
  • applied MST to infertile female mice and found that the technique could permanently correct deficiencies in the cytoplasms of poor quality egg cells, allowing the mice to give birth to healthy offspring. (elifesciences.org)
  • In attempt to develop a hormonal protocol more suitable to produce good quality oocytes for use in biotechnologies, an earlier study demonstrated that FSH applied in multiple decreasing doses produced better quality oocytes. (embrapa.br)
  • These findings open up the possibility of developing new treatments for infertility caused by problems with egg cells, so experiments involving human egg cells are now being performed to evaluate the safety and effectiveness of the technique. (elifesciences.org)
  • Expression of the pluripotent markers Sox2 and Oct4 , and the primitive germ cell markers Blimp1 and Stella were not detected. (oncotarget.com)
  • Gene expression profiles of adult renal corpusle isolated using sieving techniques. (nih.gov)
  • In conclusion, the cervical relaxation protocol based on the administration of estradiol benzoate, d-cloprostenol and oxytocin may temporarily alter the expression of NANOG and OCT4 genes in surgically. (embrapa.br)
  • They are classified as either totipotent (iTC), pluripotent (iPSC) or progenitor (multipotent - iMSC, also called an induced multipotent progenitor cell - iMPC) or unipotent - (iUSC) according to their developmental potential and degree of dedifferentiation. (wikipedia.org)
  • The presence of Zp1 , Zp2 , Zp3 and Nobox were not detected, indicating that porcine EPCs are not indicative of mature primordial oocytes. (oncotarget.com)
  • Finally, using an in vitro culture system of human FGCs, we showed that the BMP signaling pathway promoted the cell proliferation of FGCs, and regulated the WNT signaling pathway by orchestrating the chromatin accessibility of its ligand genes. (nature.com)