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EmbryosEmbryonic stemDifferentiationBiologistsGeneSomaticStanford UniversiTherapeuticGenesPluripotent StemTechnique in which the nucleusGeneticsBone marrowGeneticTissueProgenitor cellMiceGerm CellsGenetically identicalNucleiEmbryoSpeciesBiochemistryMammalianEpigeneticImmunologyOocyteRecombinantAnatomicalEuropean Molecular BiCellularApproachesTissuesHuman stem cellsIsolationExpressionEncodeResearchBiologistOrganoidsReproductive biologyLife Sciences1991CardiomyocytesSurrogate light-chainNucleusBiologicalAntigenVitro and in vivoFrontiersNuclear transferReplicationTransgenicFunctional
Embryos12
  • The activated oocytes were then transferred into the oviducts or uterine horns of recipient dogs at times appropriate to the embryos' developmental stages. (nature.com)
  • If this mysterious creature could be captured and grown in the lab, it might change the face of medicine, promising, among other remarkable options, the ability to grow replacement human tissue at will … [but] these cells are found only in embryos or very immature fetuses, and pro-life forces have targeted the researchers who are hunting for ES cells, hoping to stop their science cold. (technologyreview.com)
  • Research shows stem cells in the lab can self-assemble back into "synthetic" embryos, shockingly similar to the real thing. (technologyreview.com)
  • In an unbiased secretion cloning screen of Xenopus gastrula embryos we isolated a novel gene, designated Darmin . (xenbase.org)
  • By secretion cloning of Xenopus cleavage -stage embryos we isolated another novel protein, designated Darmin-related ( Darmin-r ) due to its sequence similarity with Darmin . (xenbase.org)
  • Embryonic stem cell research "uses special cells found in three-to-five day old human embryos to seek cures for a host of chronic disease" (PRC). (ipl.org)
  • The somatic cell and the oocyte is then fused (f) and the embryos is allowed to develop to a blastocyst in vitro (g). (biomedcentral.com)
  • Currently, the efficiency for nuclear transfer is between 0-10%, i.e., 0-10 live births after transfer of 100 cloned embryos. (biomedcentral.com)
  • NBC involves molecular biology approaches and techniques involving mouse embryonic stem cells and mouse embryos that fall within the technical repertoire of laboratories addressing neurobiological and developmental questions. (researchsquare.com)
  • We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)
  • The stem cells suits human needs, does not cause harm and can be obtained from both adult and fetal does not conflict with religious beliefs, it has tissues, umbilical cord and early embryos. (who.int)
  • Unicellular for those cells that are derived from human organisms are primed to replicate (clone) pre-embryos, which seem to have a high themselves by nature. (who.int)
Embryonic stem10
Differentiation15
  • Drs. John B. Gurdon, Irving L. Weissman, and Shinya Yamanaka have been pioneers in studying stem cells and the reprogramming of highly differentiated adult cells into pluripotent cells capable of directing differentiation from a single cell to an adult animal. (brandeis.edu)
  • Professor Campbell was a cell biologist/embryologist with a research career spanning more than 30 years, the majority of which was in the field of cell growth and differentiation. (nottingham.ac.uk)
  • His pioneering studies into cell-cycle control and cellular differentiation led to the programme of work at Roslin that gave birth to the first mammal to be cloned from adult cells - ie. (nottingham.ac.uk)
  • Five stromal-cell-dependent lymphocyte clones are described that correspond to late pre-B or early B-cell stages of differentiation. (elsevierpure.com)
  • They are useful for determining the molecular requirements for pre-B replication, for studying the stromal cells that supply those factors, and for delineating the final sequence of differentiation events as newly formed lymphocytes prepare to exit the bone marrow. (elsevierpure.com)
  • Ly-6 antigens were inducible on 2E8 and 1A9 cells, but the lymphocytes were otherwise arrested in differentiation. (elsevierpure.com)
  • The normal replication and differentiation of pre-B cells is probably regulated by complex interactions with multiple cytokines and matrix components of the marrow microenvironment. (elsevierpure.com)
  • During the differentiation process from hematopoietic progenitor cells, chromosomes in the cell nucleus aggregate and the cell becomes a mature erythrocyte by subsequently losing this nucleus (enucleation). (sciencedaily.com)
  • According to rough calculations, the amount of culture medium needed to produce enough erythrocytes for a normal transfusion pack (containing one trillion units) is 1,000 -- 2,000 liters when using direct differentiation from undifferentiated iPS cells, whereas 50-100 liters is sufficient with the newly developed method. (sciencedaily.com)
  • Despite different origin, all pluripotent lines demonstrate considerable similarity of the major biological properties: active self-renewal and differentiation into various somatic and germ cells in vitro and in vivo, similar gene expression profiles, and similar cell cycle structure. (elsevierpure.com)
  • Hox genes encode DNA-binding proteins that determine the differentiation of cells along the antero-posterior body axis, both in fruit flies and vertebrates. (xenopuswelfare.org)
  • 1. Development of a ML/AI pipeline for the prediction of subtypes and differentiation stage of developing mouse cardiac cells from mesoderm to birth and from in vitro differentiation of human induced pluripotent stem cells (hiPSCs). (stanford.edu)
  • Recent Accomplishments - Starting in 2013, we incorporated Fluidigm-based multiplex single cell PCR equipment HD Biomark to analyze the differentiation of single cardiac progenitor cells in vitro and found that Nkx2.5 expression marks a subpopulation of committed endocardial precursor cells in the mouse heart (Li et al, Development 2015). (stanford.edu)
  • Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
  • Expression increases throughout myelocyte differentiation and peaks in the mature myelomonocytic cells. (lu.se)
Biologists3
  • The method performs favorably compared to other transient reprogramming protocols, and the study will be of interest to developmental biologists as well as researchers who study ageing. (elifesciences.org)
  • Gehring was the renowned developmental biologists of those times. (wepapers.com)
  • From 2002 to 2006 he was President of the International Society of Developmental Biologists. (xenopuswelfare.org)
Gene16
  • cndp2 (CNDP dipeptidase 2 (metallopeptidase M20 family)) gene expression in Xenopus laevis embryo , assayed via in situ hybridization, NF stage 3 (4-cell), dorsal view. (xenbase.org)
  • Throughout this paper I will detail the early career of Dr. Nusslein-Volhard and the ways in which her research helped to revolutionize the field of developmental biology as it pertains to embryonic development and gene analysis/function. (wepapers.com)
  • This pioneering study has helped pave the way for others to develop gene and stem-cell based strategies for therapeutic purposes. (nottingham.ac.uk)
  • The research showed that an increase in the expression of the c-MYC gene led to an enhancement of the replication ability of erythrocyte progenitor cells. (sciencedaily.com)
  • It was also found that the expression of the BCL-XL gene was higher during the maturation process of erythrocyte progenitor cells than during their replication process. (sciencedaily.com)
  • As the c-MYC gene and BCL-XL gene are present within erythrocyte progenitor cells, methods that make use of them actually mimic the body's own system and can therefore be thought of as safer with a view to future clinical application than the previously developed systems for large-scale erythrocyte production using oncogene-derived from Human papillomavirus that are not normally expressed in the human body. (sciencedaily.com)
  • Topics covered will include cell membrane structure and function, metabolism, cell motility and division, genome structure and replication, the regulation of gene expression and protein production, genotype to phenotype relationship, and basic principles of inheritance. (middlebury.edu)
  • Introduction to core biological concepts including cell structure, cellular metabolism, cell division, DNA replication, gene expression and genetics. (vcu.edu)
  • We were the first to link Wnt signaling with adult stem cell biology, when we showed that TCF4 gene disruption leads to the abolition of crypts of the small intestine (8), and that TCF1 gene knockout severely disables the stem cell compartment of the thymus (2). (hubrecht.eu)
  • The Tcf4-driven target gene program in colorectal cancer cells is the malignant counterpart of a physiological gene program in selfrenewing crypts (13, 14). (hubrecht.eu)
  • Amongst the intestinal Wnt target genes (13), we found the Gpr49/Lgr5 gene to be unique in that it marks small cycling cells at crypt bottoms. (hubrecht.eu)
  • The Wnt target gene encoding the transcription factor Achaete scute-like 2 controls intestinal stem cell state (26). (hubrecht.eu)
  • Variants of reference strain A/SA-1 contained clones of Chlamydophila ompA , the gene that encodes MOMP, differentiate geno- abortus . (cdc.gov)
  • immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
  • In combination with transgenic organisms, such enzyme-actuated antisense tools will enable gene silencing in specific cell types, including tissues that are not amenable to optical targeting. (cdc.gov)
  • 11 , 12 Light-dependent gene silencing can be achieved through whole-organism irradiation or the targeted illumination of specific cell populations. (cdc.gov)
Somatic14
  • Successful somatic-cell nuclear transfer (SCNT) depends on the quality, availability and maturation of the animal's unfertilized oocytes. (nature.com)
  • Figure 1: Dog cloned by somatic-cell nuclear transfer. (nature.com)
  • a , Snuppy, the first cloned dog, at 67 days after birth (right), with the three-year-old male Afghan hound (left) whose somatic skin cells were used to clone him. (nature.com)
  • Recent work has demonstrated that the epigenome is already rejuvenated by the maturation phase of somatic cell reprogramming, which suggests full reprogramming is not required to reverse ageing of somatic cells. (elifesciences.org)
  • Dolly the Sheep, the first mammal to be cloned from an adult derived somatic cell, was born in 1996. (nottingham.ac.uk)
  • Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. (biomedcentral.com)
  • However, somatic cloning has been inefficient in all species in which live clones have been produced. (biomedcentral.com)
  • These developmental defects have been attributed to incomplete reprogramming of the somatic nuclei by the cloning process. (biomedcentral.com)
  • Because cattle are a species widely used for nuclear transfer studies, and more laboratories have succeeded in cloning cattle than any other specie, this review will be focused on somatic cell cloning of cattle. (biomedcentral.com)
  • Somatic cell cloning (cloning or nuclear transfer) is a technique in which the nucleus (DNA) of a somatic cell is transferred into an enucleated metaphase-II oocyte for the generation of a new individual, genetically identical to the somatic cell donor (Figure 1 ). (biomedcentral.com)
  • Schematic diagram of the somatic cloning process. (biomedcentral.com)
  • Developmental defects, including abnormalities in cloned fetuses and placentas, in addition to high rates of pregnancy loss and neonatal death have been encountered by every research team studying somatic cloning. (biomedcentral.com)
  • Permanent lines of pluripotent stem cells can be obtained from humans and monkeys using different techniques and from different sources-inner cell mass of the blastocyst, primary germ cells, parthenogenetic oocytes, and mature spermatogonia-as well as by transgenic modification of various adult somatic cells. (elsevierpure.com)
  • This cell then has therapeutic cloning: the global the capacity to divide and grow into an exact replica of the original from whom the debate somatic cell was taken. (who.int)
Stanford Universi1
Therapeutic6
  • He was the first to appreciate the therapeutic potential of stem cell therapy and has pioneered its development. (brandeis.edu)
  • Sinevici N, Harte N, O'Grady I, Xie Y, Min S, Hun Mok K, O'Sullivan J., The novel therapeutic potential of bovine "-lactalbumin made lethal to tumour cells and oleic acid in oral squamous cell carcinoma. (tcd.ie)
  • We are pleased to announce an opening for Post-Doctoral position in the field of molecular biology and therapeutic applications in breast cancer and melanoma progression. (abcd-it.org)
  • Therapeutic cloning possesses enormous potential for revolutionizing medical and thera- peutic techniques. (who.int)
  • This is therapeutic cloning. (who.int)
  • While progress has been made in recent years in the characterisation of pulmonary carcinoids, little is known about the underlying biology or developmental origins of these molecular groups, hampering efforts to identify predictive markers and suitable therapeutic options. (who.int)
Genes8
  • His demonstration that the expression of four master regulatory genes was sufficient to cause the reprogramming of adult cells has opened up many possibilities for human stem cell therapies. (brandeis.edu)
  • Applying MPTR to dermal fibroblasts from middle-aged donors, we found that cells temporarily lose and then reacquire their fibroblast identity, possibly as a result of epigenetic memory at enhancers and/or persistent expression of some fibroblast genes. (elifesciences.org)
  • Overall, when she began working in this lab with Wieschaus she discovered new ways of working on recombinant DNA and molecular biology with the focus to clone developmentally interesting genes. (wepapers.com)
  • The 2E8 cells had rearranged and expressed κ light-chain genes but displayed them on the surface along with surrogate light chains and μ heavy chains. (elsevierpure.com)
  • By transducing two genes (c-MYC and BCL-XL) into iPS cells and ES cells, a research team has succeeded in producing erythrocyte progenitor cells with almost unlimited ability to replicate in vitro, which they then differentiated successfully into mature erythrocytes. (sciencedaily.com)
  • He has cloned several genes that code for secreted antagonists of growth factors that are used by embryonic cells to form morphogen gradients. (xenopuswelfare.org)
  • Two other Wnt target genes, RNF43 and ZNRF3, encode stem cell-specific E3 ligases that downregulate Wnt receptors in a negative feedback loop (35). (hubrecht.eu)
  • To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
Pluripotent Stem5
Technique in which the nucleus1
  • The Wakayama team used a modified version of a cloning technique in which the nucleus of a mouse cell - in this case a cell from dead tissue that has been frozen and then thawed - is injected into a mouse egg that has had its nucleus removed. (newscientist.com)
Genetics6
  • She graduated with a degree in Biochemistry in 1968 and received her Doctorate of Biology/Genetics in 1973 from Eberhard-Karl University of Tubingen. (wepapers.com)
  • After graduating, she soon became clear that her future research work will be to study the developmental genetics of Drosophila. (wepapers.com)
  • After losing interest in molecular biology she decided to apply her knowledge of genetics to cellular biology, more specifically pattern formation and regeneration in the hydra, a genus of freshwater polyps. (wepapers.com)
  • The real experts to ask about the accurate scientific facts of human embryology are the scientific experts in human embryology who are academically credentialed Ph.D. human embryologists - not the "experts" in cell biology, genetics, doctors, nurses, theologians, lawyers or politicians, secretaries, news journalists, etc. (lifeissues.net)
  • The breadth of course topics available to you from VCU's Department of Biology stems directly from faculty expertise in molecular and cellular biology, terrestrial and aquatic ecology, evolutionary and conservation biology, physiology, genetics, bioinformatics, forensic science and other disciplines within the field of biology. (vcu.edu)
  • Fundamentals of human biology, including the structure, function and disorders of human body systems, principles of human genetics and inheritance, human evolution, and the interaction of humans with the environment. (vcu.edu)
Bone marrow2
  • B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)
  • Inspired from developmental processes, human mesenchymal cell lines can be programmed to form cartilage, bone and bone marrow tissues in vitro and in vivo. (lu.se)
Genetic8
  • In 1962 he made the stunning observation that it was possible to take a differentiated adult cell from a frog and to re-set its genetic program so that the reprogrammed nucleus could be implanted in an enucleated egg and direct the development of tadpoles. (brandeis.edu)
  • Globally, there are several cloning programmes that aim to increase the size of rapidly-dwindling populations of endangered species such as African wildcats, and maintain genetic diversity through one-off clonings of individuals that haven't bred . (newscientist.com)
  • To make matters worse, human ES cells could conceivably provide a vehicle for the genetic engineering of people, and the ethical dilemmas surrounding human cloning threaten to spill over onto this field. (technologyreview.com)
  • There he continued his research on the cloning and genetic modification of livestock. (nottingham.ac.uk)
  • In this introduction to modern cellular, genetic, and molecular biology we will explore life science concepts with an emphasis on their integral nature and evolutionary relationships. (middlebury.edu)
  • In the real world, there is no such thing scientifically as a "pre-embryo", or "just a genetic individual" as opposed to a "developmental individual. (lifeissues.net)
  • As part of its charge, the committee was asked to prepare a subreport evaluating methods for detecting potential unintended compositional changes across the spectrum of messenger ribonucleic acid (mRNA), proteins, metabolites and nutrients that may occur in food derived from cloned animals that have not been genetically modified via genetic engineering methods. (nationalacademies.org)
  • For cellular life to exist, genetic material must be copied and passed on to newly-divided cells. (xenopuswelfare.org)
Tissue10
  • In a surprise finding, the Wakayama team discovered that it was easiest to create clones from brain tissue, even though clones have never been created from living brain cells. (newscientist.com)
  • Wakayama speculates that freezing and thawing the tissue somehow makes it easier to "reprogram" the brain cell nucleus. (newscientist.com)
  • Brain tissue is also high in sugars, which can protect cells when they freeze. (newscientist.com)
  • But this is the first time animals have been cloned from lumps of tissue frozen without the use of chemicals that might protect the cells from damage. (newscientist.com)
  • 8 Jun, 2007 04:13 pm Stem cells provide the starting material for the development and repair of every organ and tissue in the body and they are present in all stages of life. (scitizen.com)
  • The Research Assistant will assist this researcher with a broad range of cell and molecular biology techniques, including plasmid DNA preparation, PCR, cloning, isolation and preparation of RNA for RNA-seq, RT-PCR/qPCR, cell culture, and tissue culture. (academickeys.com)
  • and altering cell and tissue characteristics for biomedical research and manufacturing. (nationalacademies.org)
  • The Organoid group, previously Clevers group, studies the molecular mechanisms of tissue development and cancer of various organs using organoids made from adult Lgr5 stem cells. (hubrecht.eu)
  • These cells represent the epithelial stem cells of the small intestine and colon (23), the hair follicle (24), the stomach (28) and many other tissue stem cell types. (hubrecht.eu)
  • One of the greatest controversies triggered tissue, a stem cell encoding for heart tissue by the rapid pace of evolution in biology, will eventually develop into heart tissue particularly in genomics and biotechnology, and so on. (who.int)
Progenitor cell2
  • We were interested in the developmental potential of single cardiac progenitor cell into cardiomyocytes, smooth muscle cells, and endothelial cells. (stanford.edu)
  • We know that innate immune cells are required for blastema progenitor cell formation, but are lacking an understanding of the immune cell processes and functions that are required for inducing and maintaining these progenitors cells. (lu.se)
Mice6
  • In 1988 he first identified and isolated the blood-forming stem cells from mice and went on to define the stages of development between the stem cells and differentiated cells of the immune system. (brandeis.edu)
  • Healthy mice have been cloned from cells from dead mice that had been frozen for 16 years, raising the possibility that endangered species could be cloned from old carcasses that have been tossed in freezers, rather than from living cells frozen using elaborate techniques. (newscientist.com)
  • Other teams have already cloned mice from previously frozen dead cells . (newscientist.com)
  • Other recent studies verified the presence of PAPP-A mRNA in granulosa cells of humans, monkeys, cattle, mice, and pigs. (bioone.org)
  • Grear and his Penn State colleagues Sarah E. Perkins, postdoctoral fellow, and Peter J. Hudson, the Verne M. Willaman chair in biology and director of the Huck Institutes of Life Sciences at Penn State, investigated the effects of increased testosterone on mice behavior. (vetscite.org)
  • Most of the lymphocyte clones were not tumorigenic in immunodeficient mice. (elsevierpure.com)
Germ Cells1
  • This paper outlines the debates prompted through a reproduction mechanism involv- by progress in cloning research, with special ing male and female germ cells. (who.int)
Genetically identical2
  • We tested whether the cloned dogs were genetically identical by microsatellite analysis of genomic DNA from the donor Afghan, the cloned dogs and the surrogates (see supplementary information ). (nature.com)
  • Analysis of eight canine-specific microsatellite loci confirmed that the cloned dogs were genetically identical to their donor dog. (nature.com)
Nuclei1
  • Later he showed that differentiated cell nuclei could give rise to fertile adult frogs. (brandeis.edu)
Embryo7
  • In addition, the federal government has barred federal dollars for human embryo research, pushing it out of the mainstream of developmental biology. (technologyreview.com)
  • C) Abundant maternal transcripts of Darmin -related ( Darmin -r) in 4-cell stage embryo . (xenbase.org)
  • In fact to get a embryonic stem cell a human embryo has to be disassembled. (ipl.org)
  • People who believe that an embryo should not be destroyed tend to say that embryonic stem cell research should not be conducted. (ipl.org)
  • Although the efficiency of nuclear transfer has been dramatically improved from the initial success rate of one live clone born from 277 embryo transfers [ 1 ], none of the aforementioned efforts abolished the common problems associated with nuclear transfer. (biomedcentral.com)
  • Nor is the embryo just a "fertilized egg", or just a "clump of cells", or appear only when the zygote is formed, or appear later after the zygote is formed, or appear after implantation - or even a week after that at 14-days. (lifeissues.net)
  • He discovered Chordin, a protein secreted by dorsal cells that binds Bone Morphogenetic Protein (BMP) growth factors, facilitating their transport to the ventral side of the embryo, where Chordin is digested by the Tolloid protease, releasing BMPs for signaling. (xenopuswelfare.org)
Species5
  • What's more, most conservationists agree that cloning should be considered only as a last resort for species such as the northern white rhino, where all other attempts at conservation have failed, says Paul Bartels, manager of BioBankSA at the National Zoological Gardens of South Africa in Pretoria. (newscientist.com)
  • Increases in the amount of PAPP-A mRNA in granulosa cells during follicular development occurs in some but not all species, indicating that other proteases or protease inhibitors may be involved in IGFBP degradation. (bioone.org)
  • However, even though nearly 30 years have passed, in fish species, the distribution of GnRH-Rs in cells and tissues, their regulation, and their functions remain elusive. (biomedcentral.com)
  • Professor Campbell was instrumental in the creation of Dolly the Sheep, the first cloned mammal, a breakthrough which paved the way for the successful cloning of many other mammal species. (nottingham.ac.uk)
  • Cisplatin induces autophagy-associated apoptosis in human oral squamous cell carcinoma (OSCC) mediated in part through reactive oxygen species. (tcd.ie)
Biochemistry1
Mammalian1
  • His research blossomed after he came to Roslin Institute where in a series of papers he put the intellectual framework into the method of mammalian cloning that ultimately led to the birth of Dolly in 1996. (nottingham.ac.uk)
Epigenetic2
  • This new type of epigenetic control has significant implications for developmental biology, evolution, unconventional computation, and synthetic bioengineering. (academickeys.com)
  • Activating mutations upstream may also underlie some epigenetic or within the ERK1/2 cascade are events that change cell signalling. (who.int)
Immunology2
  • Developmental Immunology , 1 (3), 149-161. (elsevierpure.com)
  • Recently, we have begun to address the pathogenic immune response in patients with myocarditis from treatment with immune checkpoint inhibitors (ICIs) for cancer therapy using single cell immunology tools from 10x Genomics. (stanford.edu)
Oocyte5
  • For SCNT, the chromosomes of the unfertilized canine oocytes were removed by micromanipulation, and a single donor cell was transferred into each enucleated oocyte. (nature.com)
  • Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila. (lu.se)
  • Using mosaic analysis and RNAi in the model organism Drosophila melanogaster, we show that Dystroglycan is required cell-autonomously for cellular polarity in two different cell types, the epithelial cells (apicobasal polarity) and the oocyte (anteroposterior polarity). (lu.se)
  • In Dystroglycan germline clones early oocyte polarity markers fail to be localized to the posterior, and oocyte cortical F-actin organization is abnormal. (lu.se)
  • A matured oocyte (c) is then enucleated (d) and a donor cell is transferred into the enucleated oocyte (e). (biomedcentral.com)
Recombinant2
  • Cell models, recombinant DNA technology and steroid quantification using liquid chromatography mass spectrometry have greatly facilitated investigations in the field of steroidogenesis. (bvsalud.org)
  • i) human cell models either transiently transfected with recombinant DNA or expressing endogenous steroidogenic enzymes and (ii) steroid identification and quantification using high resolution mass spectrometry. (bvsalud.org)
Anatomical2
  • Novel technology is being developed to modulate the bioelectric communication that enables cells to coordinate their activities toward specific anatomical and functional outcomes. (academickeys.com)
  • We subsequently became interested in developing a developing heart expression atlas in order to identify the cell type, developmental stage, and anatomical location of each single cardiac cells during mouse heart development using a random forest-based computational algorithm that we termed ATLAS-seq. (stanford.edu)
European Molecular Bi2
Cellular1
  • It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application. (bdbiosciences.com)
Approaches1
  • This will be coupled with next generation sequencing based approaches to understanding the consequences of perturbing immune cell function. (lu.se)
Tissues4
  • 27 Jun, 2007 06:08 pm Stem cells have the potential to become all the cells and tissues in the human body. (scitizen.com)
  • We also use human stem cells as a test-tube model to study the process of heart formation and to create new human heart tissues and organs from these stem cells. (stanford.edu)
  • In mammals, physiological Wnt signaling is intimately involved with the biology of adult stem cells and self-renewing tissues (18,19). (hubrecht.eu)
  • These epithelial organoid cultures are genetically and phenotypically extremely stable, allowing transplantation of the cultured offspring of a single stem cell, as well as disease modeling by growing organoids directly from diseased patient tissues (32, 47, 53). (hubrecht.eu)
Human stem cells3
  • His laboratory has also identified human stem cells including human leukemia stem cells that have helped to form the concept of cancer stem cells. (brandeis.edu)
  • 6 Sep, 2007 12:57 pm British authorities decided yesterday to permit research that uses animal eggs to create human stem cells because of the limited supply of human eggs. (scitizen.com)
  • Embryonic stem cell transplants have been an ethical, social, and legal controversy since the first successful transplant of human stem cells in 1998. (ipl.org)
Isolation1
  • Additional experience in isolation and culture of primary cells, mass spectrometry and bioinformatic analyses would be considered advantageous. (abcd-it.org)
Expression2
  • Thus, expression of authentic light chain need not coincide with termination of surrogate light-chain utilization in newly formed B cells. (elsevierpure.com)
  • Expression detected in several myeloid leukemic, colon cancer, and prostate cancer cell lines. (lu.se)
Encode1
  • When the iv mouse mutation was cloned, it was found to encode a molecular motor protein, an axonemal dynein, and was named lrd , for left-right dynein (human homolog is DNAH11 / DNAHC11 , Dynein heavy chain 11 , axonemal ). (medscape.com)
Research20
  • The 2009 Rosenstiel Award for Distinguished Work in Basic Medical Science will be awarded to three pioneers in stem cell research. (brandeis.edu)
  • The finding also raises hopes of one day being able to resurrect extinct animals frozen in permafrost, such as the woolly mammoth, says Teruhiko Wakayama of the RIKEN Center for Developmental Biology in Kobe, Japan, who led the research. (newscientist.com)
  • Now research on ES cells is paid for by the US government. (technologyreview.com)
  • So when I was recently contacted by an earnest and amiable member of a local school board who was concerned about the questionable manner in which the issue of "stem cell" research - both human embryonic and adult - was presented to the high school students in his district in a currently-used science textbook, I agreed to evaluate that section in the text for him. (lifeissues.net)
  • My edited analysis of the section on "stem cell research" in this science textbook is copied below. (lifeissues.net)
  • In my opinion there is no question but that the scientific information on stem cell research included in this science text book being used in Illinois schools incorporates some inaccurate scientific facts, and seems to be very partial to the use of human embryonic "stem cell" research. (lifeissues.net)
  • 8 Feb, 2008 06:09 pm Six years ago, biomedical engineer Michael King was exploring the strange rolling motion of white blood cells when his research took a radical turn. (scitizen.com)
  • 27 Nov, 2007 10:41 am Tony Maciulis talks with Dr. Jon LaPook about a breakthrough in stem cell research. (scitizen.com)
  • 21 Nov, 2007 10:54 am A huge advancement in stem cell research--and a stake in the heart of human cloning--was announced yesterday. (scitizen.com)
  • 25 Jun, 2007 04:43 pm On June 7, the House of Representatives voted 247-176 to pass a bill (S 5) that would allow federal funding for research using stem cells derived from. (scitizen.com)
  • Inevitably most people will remember him for Dolly the sheep although his recent work was focused on fundamental and applied stem cell research as a tool for the study of human disease. (nottingham.ac.uk)
  • STEM CELL RESEARCH is a very controversial topic in today's time. (ipl.org)
  • Stem cell research is not worth supporting. (ipl.org)
  • Advocates of stem cell research believe that the cells are not equivalent to human life because it is inside the womb even facing the fact that the start of a human life is in the moment of conception. (ipl.org)
  • While many people say the use of the cell research is a way to advance medical knowledge and expand treatments, there is no guarantee that the treatments will work. (ipl.org)
  • In recent years, several competing viewpoints have emerged about embryonic stem cell research. (ipl.org)
  • All of this debate raises an important question, Should embryonic stem cell research be conducted for treatment of present and future diseases? (ipl.org)
  • On the other hand, people who believe that embryonic stem cell research creates means of curing diseases reply that the research should be conducted. (ipl.org)
  • Research experience in molecular biology (including molecular cloning, DNA, RNA and protein manipulations) as well as an extensive experience in cell biology and cell imaging techniques are required. (abcd-it.org)
  • At International Society for Stem Cell Research-the big stem cell meeting-Yamanaka revealed one more factor. (medscape.com)
Biologist1
  • I had heard whispers that a very accomplished developmental biologist, Douglas Melton, was actually starting to launch into the area. (medscape.com)
Organoids2
  • Long-term clonal culturing of organoids from Lgr5 stem cells. (hubrecht.eu)
  • Based on these combined insights, we have established Lgr5/R-spondin-based culture systems that allow the outgrowth of single mouse or human Lgr5 stem cells into ever-expanding organoids. (hubrecht.eu)
Reproductive biology1
Life Sciences2
19911
  • In 1991, we reported the cloning of a T cell specific transcription factor that we termed TCF1 (1). (hubrecht.eu)
Cardiomyocytes1
  • 16 , 17 In particular, transgenic zebrafish lines that express nfsB in β cells, cardiomyocytes, or other cell types have been established, and their exposure to the metronidazole leads to the targeted loss of these cells. (cdc.gov)
Surrogate light-chain1
  • We now show that one member of this family (approximately 33 kD) was associated with the μ+surrogate light-chain complex on the 1A9 pre-B-cell clone. (elsevierpure.com)
Nucleus3
  • When the nucleus of a stem cell has been the technique of cloning. (who.int)
  • The con- is removed and replaced by a nucleus of cept of human cloning has long been in the another cell type, the stem cell will then imagination of many scientists, scholars and be reprogrammed to produce the product fiction writers [ 1 ]. (who.int)
  • The basic techniques of of the implanted nucleus, when it fully cloning have been known for some time, and develops. (who.int)
Biological4
  • The Levin Lab at Tufts (see http://allencenter.tufts.edu/ ) is dedicated to the advancement of basic biology and biomedicine, and to using an integrated, multidisciplinary approach to control biological structure and function. (academickeys.com)
  • However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. (bdbiosciences.com)
  • As the analysis of these data is computationally intensive, we have expanded our technology platform to incorporate biocomputational work including machine learning/artificial intelligence (ML/AI) to enhance our understand of the biological meaning from our single cell RNA seq data. (stanford.edu)
  • 1974. Biological handbooks: Biology data book. (cdc.gov)
Antigen1
  • When compared to mature B lymphomas, fewer bands coprecipitated with the surface-labeled Ig isolated from pre-B- and early B-cell lines, suggesting that components of the antigen receptor are sequentially acquired during development. (elsevierpure.com)
Vitro and in vivo1
  • This project will delve into innate immune cell function, establishing in vitro and in vivo systems test innate immune cell function in regeneration. (lu.se)
Frontiers1
Nuclear transfer3
  • Here we describe the cloning of two Afghan hounds by nuclear transfer from adult skin cells into oocytes that had matured in vivo . (nature.com)
  • Various strategies have been employed to modify donor cells and the nuclear transfer procedure in attempts to improve the efficiency of nuclear transfer. (biomedcentral.com)
  • The advent of techniques to propagate animals by nuclear transfer, also known as cloning, potentially offers many important applications to animal agriculture, including reproducing highly desired elite sires and dams. (nationalacademies.org)
Replication2
  • Hematopoietic progenitor cells, however, still have self-replication ability, and if the factors involved in this self-replication could be identified, it might become possible to induce virtually unlimited replication of progenitor cells. (sciencedaily.com)
  • However, it appears that the ability of the In its simplest form, cloning is defined stem cells to transform is limited, except as the exact replication of cells. (who.int)
Transgenic2
  • It is, however, important to distinguish the use of bST from other biotechnologies, such as transgenic or cloned animals. (nationalacademies.org)
  • Transgenic biology provides a means of altering animal genomes to achieve desired production and health outcomes of commercial value and societal importance. (nationalacademies.org)
Functional1
  • The efficiency of lymphocyte growth at limiting dilution varied substantially on different stromal-cell clones and may reflect functional heterogeneity of stromal cells. (elsevierpure.com)