• Huang J.C., Lei Z.L., Shi L.H., Miao Y.L., Yang J.W., Ouyang Y.C., Sun Q.Y., Chen D.Y., Comparison of histone modifications in in vivo and in vitro fertilization mouse embryos. (biologie-journal.org)
  • SECM technique may be a valuable tool for accurately assessing the quality of embryos and thereby contribute to improving outcomes associated with assisted reproduction, including human in vitro fertilization. (bioone.org)
  • in vitro fertilization. (who.int)
  • In the last decade, in vitro fertilization and sperm capacitation techniques have significantly advanced. (vetmedmosul.com)
  • This has been shown for intrauterine insemination (IUI), in vitro fertilization (IVF), and ICSI, and increased incidences of chromosomal abnormalities, minor and major birth defects, or early childhood cancers, particularly in the male offspring, have been linked to these procedures [ 8 - 16 ]. (hindawi.com)
  • Despite considerable technological advancements, polyspermy remains a significant challenge in in-vitro fertilization (IVF) procedures in pigs, disrupting normal embryonic development. (e-jarb.org)
  • Usage of melatonin during in vitro fertilization (IVF) procedures has been found to improve oocyte quality, survival, and fecundity. (jrhm.org)
  • In addition, pyruvate (0.33 mmol/L) and lactate (3.3 mmol/L) together were beneficial to early embryonic development in sheep[ 3 ]. (biomedcentral.com)
  • Christians E., Rao V.H., Renard J.P., Sequential acquisition of transcriptional control during early embryonic development in the rabbit. (biologie-journal.org)
  • 2- Melatonin has a beneficial impact on embryonic development. (vetmedmosul.com)
  • Following germinal vesicle break down (GVBD) the oocyte is transcriptionally quiescent until fertilization and activation of the embryonic genome, occurring at the four-cell stage of development in the pig [ 9 ]. (biomedcentral.com)
  • These results suggest that maintaining MPF activity after cryopreservation may have a positive effect on further embryonic development, but is unable to fully overcome cryoinjury. (molcells.org)
  • Paternal sperm DNA damage has negative effects on the integrity of early embryonic development as the percentage of good quality embryos as well as implantation rates was significantly reduced in patients exhibiting high DNA damage [ 27 ] and it appears that the second and third mitoses are the sensitive periods [ 28 ]. (hindawi.com)
  • It is only through understanding embryonic metabolism and development that we can derive and maintain different in vitro stem cell states for disease modeling and therapies. (conditionmed.org)
  • Collectively, these results demonstrate that various fertilization conditions, including modified co-culture period, active sperm separation, and culture medium volume, enhance fertilization efficiency and subsequent embryonic development by decreasing polyspermy occurrence. (e-jarb.org)
  • Our analysis of mouse development from embryonic day 5 to 15 (E5 to E15) has demonstrated that synemin M mRNA is produced at E5 as early as nestin and vimentin mRNA, prior to the appearance of the H isoform. (biomedcentral.com)
  • The respiratory system does not carry out its physiological function (of gas exchange) until after birth, though the respiratory tract, diaphragm and lungs do begin to form early in embryonic development and continue through fetal development, only functionally maturing just before birth. (edu.au)
  • The bovine embryos with higher oxygen consumption are better candidates to further development into good quality embryos and yielded higher pregnancy rates after embryo transfer. (bioone.org)
  • This study was conducted to evaluate in vivo and in vitro development of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection. (bioone.org)
  • In vivo development was assessed after transfer of injected oocytes to the oviducts of recipient mares. (bioone.org)
  • The production of CO2 from glucose by sheep embryos has been determined[ 3 ] and results have indicated that little glucose is utilized by the embryos of sheep and that blastocysts derived from oocytes, matured and fertilized in vitro , oxidized glucose at a lower rate than those developed in vivo . (biomedcentral.com)
  • 1998. Influence of organochlorine pesticides on maturation and post-fertilization development of bovine oocytes in vitro . (cdc.gov)
  • Mammalian ovaries are endowed with a huge number of small oocytes (primordial oocytes) in primordial follicles. (go.jp)
  • oxidative stress is the most prominent one, which will cause damage or alter the genetic material of the sperm and reduce the quality of the oocytes, and has a crucial impact on the possibility of developing embryos even after implantation. (vetmedmosul.com)
  • The oocytes were fertilized by spermatozoa that had been capacitated with two groups of fertilization media, the first group containing melatonin and the second group containing cysteamine. (vetmedmosul.com)
  • In conclusion, melatonin could be a promising tool for improving sperm competence for fertilizing oocytes and embryo development in sheep. (vetmedmosul.com)
  • Oocytes, sperms, and embryos are shielded from oxidative stress in their natural environment by free radical scavengers found in oviductal and follicular fluids. (vetmedmosul.com)
  • Oocytes and embryos are not provided with this level of protection when in vitro production (9). (vetmedmosul.com)
  • Melatonin supplementation has been shown to promote bovine and porcine oocytes' maturation rate and increase their blastocyst of IVF embryos (16). (vetmedmosul.com)
  • Collected MII oocytes were vitrified and divided into four groups: untreated, 10 mM caffeine (CA), 10 μM MG132 (MG), and 10 mM caffeine +10 μM MG132 (CA+MG). After warming, the MPF activity of oocytes and their blastocyst formation and implantation rates in the CA, MG, and CA+MG groups were much higher than those in the untreated group. (molcells.org)
  • Cryopreservation of mammalian oocytes has been successfully applied for long-term storage of surplus eggs in mammalian assisted reproductive technology (ART), including in humans. (molcells.org)
  • On the one hand, reactive oxygen species (ROS) are mandatory mediators for essential cellular functions including the function of germ cells (oocytes and spermatozoa) and thereby the fertilization process. (hindawi.com)
  • These protease-induced decreases in IGFBP-4 and -5 likely cause increased levels of bioavailable (or free) IGFs that stimulate steroidogenesis and mitogenesis in developing dominant follicles, which ultimately prepare the follicle(s) and oocyte(s) for successful ovulation and fertilization. (bioone.org)
  • Oocyte quality is of utmost importance for the early embryo to develop to term for both fertilized and cloned embryos. (engineering.org.cn)
  • ROS are free radicals that are continuously produced by oxidative metabolic reactions and increase in vitro conditions, causing aging of both the oocyte (7) and sperm (8), as well as perhaps impairing fertilization and embryo development. (vetmedmosul.com)
  • Maturation of the mammalian oocyte is a complex process involving internal checkpoints and bidirectional communication with the surrounding cumulus cells. (biomedcentral.com)
  • The oocyte must maintain arrest at the diplotene stage until meiotic resumption occurs. (biomedcentral.com)
  • Oocyte arrest at the diplotene stage is maintained in part through the activity of phosphodiesterase enzymes that catalyze the hydrolysis of cyclic adenosine monophosphate (cAMP) into AMP [ 1 , 2 ]. (biomedcentral.com)
  • The inability to transcribe mRNA during this stage of development and the probable necessity for post-transcriptional gene regulation (PTGR) suggests an important role for non-coding RNA in the maturing oocyte. (biomedcentral.com)
  • Another drawback of oocyte vitrification is diminished developmental potential because of the decreased quality of embryos after warming. (molcells.org)
  • In this paper, we describe a scanning electrochemical microscopy (SECM) technique that is a non-invasive and sensitive method for measuring oxygen consumption by individual embryos. (bioone.org)
  • We showed that the extracted criteria could be used to evaluate the differences between individual embryos. (nature.com)
  • Abdalla H., Yoshizawa Y., Hochi S., Active demethylation of paternal genome in mammalian zygotes. (biologie-journal.org)
  • The mammalian genome comprises nuclear DNA (nDNA) derived from both parents and mitochondrial DNA (mtDNA) that is maternally inherited and encodes essential proteins required for oxidative phosphorylation. (regenerativemedicine.net)
  • Thus, we investigated male mouse (m) PRL protein and mRNA expression in spermatozoa at various differentiation stages in the testes. (go.jp)
  • The mammalian ovary has all the molecular machinery required for the biosynthesis of melatonin and interestingly concentration of melatonin in the follicular fluid of pre-ovulatory follicles is much higher than circulatory melatonin even in humans. (jrhm.org)
  • Respiration activity correlates with the embryo quality. (bioone.org)
  • and examining the effect of the co-culture medium ( Abeydeera and Day, 1997 ) on embryo production. (e-jarb.org)
  • Fulka H., St John J.C., Fulka J., Hozak P., Chromatin in early mammalian embryos: achieving the pluripotent state. (biologie-journal.org)
  • The developing embryo, from which pluripotent stem cells originate, undergoes a series of dynamic metabolic transitions synchronized to its molecular development. (conditionmed.org)
  • Rieger[ 4 ] inferred that the pre-implantation bovine embryo undergoes an intensified period of cellular synthetic processes which require appropriate substrates for production of energy and reducing equivalents in the form of nicotinamide-adenine dinucleotide phosphate. (biomedcentral.com)
  • Within the uterus, such signals support implantation [ 2 ] while in the periphery they induce and/or maintain tolerance without allowing for deleterious immune suppression to occur [ 3 ]. (biomedcentral.com)
  • Mares were killed 7.5-8.5 days after transfer and the uterus and oviducts flushed for embryo recovery. (bioone.org)
  • In single bovine IVF-derived embryos, PIF levels in medium at day 3 of culture were higher than non-cleaving embryos (control) (P = 0.01) and at day 7 were higher than day 3 (P = 0.03). (biomedcentral.com)
  • Test FITC-PIF uptake by cultured bovine blastocysts using fluorescent microscopy. (biomedcentral.com)
  • FITC-PIF was taken-up by cultured bovine blastocysts, but not by scrambled FITC-PIF (control). (biomedcentral.com)
  • The synchronization and ovulatory responses of Sangsari cross bred ewes and metabolism of energy substrates in 8-cell stage embryos to hatched blastocysts stage produced in vitro or in vivo were investigated. (biomedcentral.com)
  • Chromosomal mosaicism in human blastocysts : the ultimate challenge of preimplantation genetic testing? (ugent.be)
  • In some species (e.g., bovine), evidence indicates that decreases in IGFBP-4 and -5 levels in estrogen-dominant preovulatory follicles are likely due, in part, to increased protease activity, whereas lower levels of IGFBP-2 are not due to increased proteolysis. (bioone.org)
  • Increased IGFBP-4 and -5 protease along with lower amounts of IGFBP-4 binding activity and greater amounts of free IGF-I are some of the earliest developmental changes documented in bovine growing antral follicles. (bioone.org)
  • Increases in the amount of PAPP-A mRNA in granulosa cells during follicular development occurs in some but not all species, indicating that other proteases or protease inhibitors may be involved in IGFBP degradation. (bioone.org)
  • In 1984, researchers identified a site that was specifically methylated-the 3′ untranslated region (UTR) of bovine prolactin mRNA. (pharmaceuticalintelligence.com)
  • This review summarizes evidence for the role of proteolytic enzymes that degrade and inactivate insulin-like growth factor binding proteins (IGFBP) during follicular development in mammals. (bioone.org)
  • PIF levels in mouse embryo culture medium significantly increased from the morula to the blastocyst stage (ANOVA, P = 0.01). (biomedcentral.com)
  • The first marked increase in the metabolism of glucose by ovine embryos was detected in compact morula stage, but there was no significant increase in the oxidation of glucose after the morula stage. (biomedcentral.com)
  • At the 8-cell stage, the embryo becomes compact, and the cells form a spherical mass called a morula. (nature.com)
  • The space inside the embryo spreads, and the morula becomes a blastocyst. (nature.com)
  • Giraldo A.M., Hylan D.A., Ballard C.B., Purpera M.N., Vaught T.D., Lynn J.W., Godke R.A., Bondioli K.R., Effect of epigenetic modifications of donor somatic cells on the subsequent chromatin remodeling of cloned bovine embryos. (biologie-journal.org)
  • Microarray-based global gene expression analyses on bovine placenta and trophoblast cells suggest that the expression profiles of specific genes depend on the cells and tissues in which they are expressed as well as the time of the gestation period. (bioone.org)
  • After intracytoplasmic sperm injection (ICSI), 48 embryos were evaluated on day 3 of their development, according to their cell number. (who.int)
  • Respiration is useful parameter for evaluating embryo quality as it provides important information about metabolic activity. (bioone.org)
  • 2019). Akin to the dynamic nutrient requirements of the developing embryo, discrete in vitro cell states have distinct metabolic profiles (Zhou et al. (conditionmed.org)
  • PIF is an early embryo viability marker that has a direct supportive role on embryo development in culture. (biomedcentral.com)
  • Meanwhile, early embryo development is significant for somatic cell nuclear transfer in cattle as a large proportion of cloned embryos fail to develop beyond peri-implantation stage. (engineering.org.cn)
  • The robust efficiency of genomic editing tools also presents an alternative approach to the functional annotation of genes critical to early development. (engineering.org.cn)
  • Haaf T., The battle of the sexes after fertilization: behaviour of paternal and maternal chromosomes in the early mammalian embryo. (biologie-journal.org)
  • 3- Spermatozoa may have a more significant impact on determining early embryos' fate. (vetmedmosul.com)
  • Using QCANet, we were able to extract several quantitative criteria of embryogenesis from 11 early mouse embryos. (nature.com)
  • In early embryos, cells are loosely connected to each other. (nature.com)
  • For time-lapse observation of early-stage D rosophila embryos, Keller et al. (nature.com)
  • Here, we consider the metabolism of the early embryo through development, and look at the nutrient milieu within the developing stem cell niche. (conditionmed.org)
  • Furthermore, sperm DNA damage appears to be linked to the most important checkpoints of fertility such as reduced fertilization rates, lower embryo quality and pregnancy rates, higher miscarriage rates, malformations, and childhood diseases [ 22 ]. (hindawi.com)
  • SECM measuring system can non-invasively measure respiration activity by single embryos of several species including human. (bioone.org)
  • Postnatal development of respiration. (edu.au)
  • The search to identify embryo-specific markers which reflect viability of cultured embryos by assessing the medium or by performing an embryo biopsy (beyond morphological evaluation) has been ongoing. (biomedcentral.com)
  • Due to its clinical applications success of IVF results is the morphological and the expansion of non-invasive treatment quality of the embryo. (who.int)
  • Our study shows a developmental stage specific regulation of synemin isoforms in ES cells and its neural derivatives. (biomedcentral.com)
  • Determine PIF levels in culture medium of multiple mouse and single bovine embryos cultured up to the blastocyst stage using PIF-ELISA. (biomedcentral.com)
  • To determine the role of PIF in post-fertilization embryo development, we measured the peptide's concentration in the culture medium and tested endogenous PIF's potential trophic effects and direct interaction with the embryo. (biomedcentral.com)
  • PIF-ELISA use to assess IVF embryo quality prior to transfer is warranted. (biomedcentral.com)
  • Understanding the association between Cell-free DNA levels in embryo CM and the quality of embryo cleavage could help improve the quality of IVF techniques. (who.int)
  • This prospective study was conducted with 96 spent CM from patients undergoing IVF cycle, in order to determine relationships of Cell-free DNA levels in embryo CM with embryo cleavage quality on day 3. (who.int)
  • In vivo and in organized cells, and proper symmetry are healthy individuals, macrophages can characteristics of higher-quality embryos, which phagocytize DNA that has been passively point to healthy development and higher rates of released into the blood from apoptotic or necrotic implantation. (who.int)
  • Along with polyspermy, the suboptimal quality of IVF-derived pig embryos poses another challenge that needs to be addressed for in vitro production systems. (e-jarb.org)
  • Superovulation ensures recovery of multiple embryos during one estrous cycle, thus allowing us to maximally exploit the female germ pool. (biomedcentral.com)
  • However, these techniques have to be carried out very carefully as they, if applied wrongly, bear risks of generating ROS damaging the germ cells and preventing fertilization. (hindawi.com)
  • Given the current state of research, comprehensive information on the impact of different conditions on IVF and embryo development would greatly contribute to our understanding of how processing methods influence IVF outcomes. (e-jarb.org)
  • This study contributes to the development of fundamental approaches for assessing embryogenesis on the basis of extracted quantitative criteria. (nature.com)
  • Musculoskeletal development - contributes the mechanical elements of ribs, intercostals and diaphragm required for breathing. (edu.au)
  • Measurement of free radicals in the culture medium of developing embryos has been reported as an index for embryo viability. (biomedcentral.com)
  • Compared with other groups, sperms treated with melatonin demonstrated hyperactivity, and the fertilization rate was significantly increased. (vetmedmosul.com)
  • The in vitro embryo production (IVEP) is very stressful for gametes. (vetmedmosul.com)
  • Day 2 and day 3 CM corresponding to each one of the embryos was analyzed, by quantitative PCR, for estimation of Cell-free DNA levels. (who.int)
  • Surfactant development - allows lung inflation and decreases the work of breathing and also related to immunology of the lungs. (edu.au)
  • Examine the inhibitory effects of anti-PIF-monoclonal antibody (mAb) added to medium on cultured mouse embryos development. (biomedcentral.com)
  • Detectable - though low - PIF levels were secreted already by 2-cell stage mouse embryos. (biomedcentral.com)
  • Adenot P.G., Szollosi M.S., Geze M., Renard J.P., Debey P., Dynamics of paternal chromatin changes in live one-cell mouse embryo after natural fertilization. (biologie-journal.org)
  • Adenot P.G., Mercier Y., Renard J.P., Thompson E.M., Differential H4 acetylation of paternal, maternal chromatin precedes DNA replication, and differential transcriptional activity in pronuclei of 1-cell mouse embryos. (biologie-journal.org)
  • Aoki F., Worrad D.M., Schultz R.M., Regulation of transcriptional activity during the first , and second cell cycles in the preimplantation mouse embryo. (biologie-journal.org)
  • Bouniol C., Nguyen E., Debey P., Endogenous transcription occurs at the 1-cell stage in the mouse embryo. (biologie-journal.org)
  • Influence of organochlorine pesticides on development of mouse embryos in vitro . (cdc.gov)
  • We showed that QCANet can be applied not only to developing mouse embryos but also to developing embryos of two other model species. (nature.com)
  • In the mouse, a transporting epithelium is established around the 8-16-cell stage through a process known as compaction where cell definition is lost and the outer cells of the embryo form tight junctions, giving rise to the blastocyst. (conditionmed.org)
  • Because the cells in embryos are considerably crowded, an algorithm to segment individual cells in detail and accurately is needed. (nature.com)
  • Kopecny V., Fléchon J.E., Camous S., Fulka J. Jr., Nucleologenesis , and the onset of transcription in the eight-cell bovine embryo: fine-structural autoradiographic study. (biologie-journal.org)
  • While the pattern of oxidation was similar among in vitro and in vivo derived embryos, a low pyruvate to lactate ratio was the preferred substrate of embryos derived in vitro . (biomedcentral.com)
  • Surgical embryo recovery was performed on Days 4 and 6 after onset of estrus (Day 0) and recovered embryos were subjected to comparative metabolism studies with in vitro derived embryos at the same stage of development. (biomedcentral.com)
  • 2020). This places metabolism at the forefront of development and cell state decisions. (conditionmed.org)
  • In this essay I will discuss recent developments in postgenomic molecular biology from the perspective of the interconnective cosmology of Greek mythology. (demul.nl)
  • A current project in the Massachusetts Experiment Station studied organic and conventional fertilization of vegetable crops in relation to productivity and elemental nutrient composition for human nutrition. (umass.edu)
  • Braude P., Bolton V., Moore S., Human gene expression first occurs between the four- , and eight-cell stages of preimplantation development. (biologie-journal.org)
  • The development of the human blood-CSF-brain barrier. (cdc.gov)
  • The purpose of this review is to summarize science-based new treatments for human reproductive failure and future developments. (infertile.com)
  • and human spermatogenic stem cell culture to treat azoospermia, and to preserve fertility in pre-pubertal boys undergoing cancer treatment. (infertile.com)
  • Couldrey C., Lee R.S., DNA methylation patterns in tissues from mid-gestation bovine foetuses produced by somatic cell nuclear transfer show subtle abnormalities in nuclear reprogramming. (biologie-journal.org)
  • Bellier S., Chastant S., Adenot P., Vincent M., Renard J.P., Bensaude O., Nuclear translocation , and carboxyl-terminal domain phosphorylation of RNA polymerase II delineate the two phases of zygotic gene activation in mammalian embryos. (biologie-journal.org)
  • However, only a tiny percentage of Assisted reproductive technology (ART)- produced embryos develop fully (1). (vetmedmosul.com)
  • In non-cleaving embryos culture medium was similar to medium alone (control). (biomedcentral.com)
  • As a result, developing a culture medium capable of effectively reducing ROS in vitro embryo production (IVEP) protocols is of tremendous importance (10). (vetmedmosul.com)
  • As for the IVF medium containing melatonin, it was superior to cysteamine in embryo development rates. (vetmedmosul.com)