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  • quantitative
  • Saliva has shown significant potential for use in non-invasive diagnostics, but the development of off-the-shelf saliva diagnostic kits requires best practices for sample preparation and quantitative insight on the availability of biomarkers and the dynamics of immunoassay in saliva. (biomedcentral.com)
  • Here, we report the development of two immuno-disaggregation bioassays using functionalized microparticles for the quantitative analysis of the cell secretome. (thno.org)
  • ligands
  • Total internal reflection from a planar side wall of a well of a multiwell plate is used to create an evanescent field in the plane of a pattern of ligands immobilized on the wall. (google.com)
  • analyte
  • The enzyme catalyzes the conversion of the inactive reductive species to an active reducing agent, which in turn reduces the metal ion to a metal atom thereby providing an enhanced means of detecting the analyte via metal deposition. (google.co.uk)
  • 4. The kit of claim 1 wherein the specific binding member for the analyte of interest is an antibody. (google.co.uk)
  • The invention concerns a method for the detection of an analyte in a sample liquid by an enzyme-immunoassay in which an enzyme-labelled compound is partitioned between a solid and a liquid phase and the amount of enzyme label in the liquid phase outside the solid phase is determined as a measure of the. (google.co.uk)
  • substrates
  • Recently, imaging ellipsometry has been demonstrated, using a light source to illuminate an entire surface and employing a two-dimensional array for detection, thus measuring the surface properties for each point of the entire surface in parallel(G. Jin, R. Janson and H. Arwin, "Imaging Ellipsometry Revisited: Developments for Visualization of Thin Transparent Layers on Silicon Substrates," Review of Scientific Instruments, 67(8), 2930-2936, 1996). (google.com)
  • wherein
  • 7. A method according to claim 5 wherein said binding moiety is an antibody or an antibody fragment. (google.com)
  • 22 . The method as in claim 20 , wherein the receptive material is an antibody. (google.com.au)
  • 2. The kit of claim 1 wherein the specific binding member is immobilized on a solid support. (google.co.uk)
  • 6. The kit of claim 1 wherein the enzyme label is a phosphatase. (google.co.uk)
  • 15. The kit of claim 14 , wherein the second member of the specific binding pair is an antibody. (google.co.uk)
  • 16. The kit of claim 14 , wherein the enzyme is selected from the group consisting of alkaline phosphatase and acid phosphatase and the redox-inactive reductive species is selected from the group consisting of hydroquinone mono- and di-phosphates, naphthohydroquinone mono- and di-phosphates, and anthrahydroquinone mono- and di-phosphates. (google.co.uk)
  • 17. The kit of claim 14 , wherein the enzyme is selected from the group consisting of alkaline phosphatase and acid phosphatase and the redox-inactive reductive species is selected from the group consisting of sesamol phosphate, eugenol phosphate and alpha-tocopherol phosphate. (google.co.uk)
  • 7. The method of claim 1 wherein the enzyme-labelled immunologically active compound of step (b) is provided as a separate liquid reagent. (google.co.uk)
  • 11. The method of claim 1 wherein the contacting step (c) comprises first contacting the liquid sample with the enzyme-labelled immunologically active compound provided as a separate liquid reagent to form a first liquid phase, and contacting the first liquid phase with the porous solid phase. (google.co.uk)
  • Lysozyme
  • In a recent series of experiments, Ratner and his colleagues used a new radiofrequency-plasma glow-discharge process to imprint a polysaccharide-like film with nanometer-sized pits in the shape of such biologically useful protein molecules as albumin (the most common blood protein), fibrinogen (a clotting protein), lysozyme and ribonuclease (two important enzymes), and immunoglobulin (antibodies). (biology-online.org)
  • protein
  • The covalent attachment of ubiquitin to a target protein proceeds through a multi-enzyme cascade. (aacrjournals.org)
  • The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. (thermofisher.com)
  • We evaluated the feasibility of our developed immuno-disaggregation bioassays using antibody-conjugated MPs and protein-conjugated MPs for the detection of target cell secretome protein. (thno.org)
  • Additionally, the immuno-disaggregation bioassays using antibody-conjugated MPs and protein-conjugated MPs have a wide detection range from 0.01 ng/mL to 100 ng/mL and 0.5 ng/mL to 100 ng/mL, respectively. (thno.org)
  • The sensitivity of the bioassay using antibody-conjugated MPs was approximately one order of magnitude higher than the bioassay using protein-conjugated MPs. (thno.org)
  • This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells transfected with Myc-tagged protein. (cellsignal.com)
  • rabbit
  • From there we will identify the main differences between the most popular types of antibodies, including mouse and rabbit monoclonals, and rabbit polyclonals. (brighttalk.com)
  • Chloramphenicol standard or sample, enzyme-marked chloramphenicol and rabbit anti-chloramphenicol antibodies are added to microwells. (medicpdf.com)
  • amplification
  • It is broken down into different techniques, with the most prevalent being: LAMP (loop-mediated isothermal amplification), NEAR (nicking enzyme amplification reaction), SDA (strand displacement amplification) and HDA (helicase-dependent amplification). (foodsafetynews.com)
  • Bacillus
  • The linker can be cleaved by a proteinase enzyme manufactured by Pseudomonas aeruginosa, a Gram-negative bacillus that causes inflammation and urinary tract infection, folliculitis, and otitis externa in humans. (biology-online.org)
  • detection of target
  • Accordingly, the present invention provides compositions comprising supports comprising surfaces to which the binding moieties (e.g. antibodies) are attached for the detection of target analytes (e.g. pathogens) as well as methods and compositions relating to the attachment of such binding moieties. (google.com)
  • biological
  • The RESIST Group at the Welsh School of Pharmacy at Cardiff University and others have looked at how molecularly imprinted polymers could be medically useful in clinical applications such as controlled drug release, drug monitoring devices, and biological and antibody receptor mimics. (biology-online.org)
  • species
  • This antibody is expected to exhibit the same species cross-reactivity as the unconjugated IRF-3 (D9J5Q) Mouse mAb #10949. (cellsignal.com)
  • Kits
  • Combined with diagnostic kits development team, we are able to develop the kits with clinical diagnostic level, and make the quality in the leading place worldwide. (cusabio.com)