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  • study of biofilm
  • In the study of biofilm diseases ranging from otitis media to prostatitis, it was found that direct microscopy and DNA- and RNA-based molecular methods regularly document the presence of living bacteria in tissues and samples that are culture negative. (springer.com)
  • Pseudomonas
  • A. López-Sánchez, A. Jiménez-Fernández, P. Calero, L. D. Gallego & F. Govantes (2013) New methods for the isolation and characterization of biofilm-persistent mutants in Pseudomonas putida . (cabd.es)
  • This comparison is performed on Pseudomonas Aeruginosa biofilms grown in transparent glass capillaries which allows for the use of both imaging modalities. (imft.fr)
  • form biofilms
  • Indeed, S. aureus is a leading cause of a variety of diseases ranging from skin and soft tissue infections to more serious illnesses, including endocarditis, necrotizing pneumonia, and osteomyelitis ( 3 - 5 ), and its ability to form biofilms is an important determinant of virulence in many of these infections ( 6 ). (asm.org)
  • The ability of the A. hydrophila AH-1 N strain to form biofilms in a continuous-flow chamber was compared with isogenic ahyI and ahyR mutants. (nih.gov)
  • Furthermore, the rbmC bap1 double deletion mutant was unable to form biofilms at the air-liquid interface after 2 days, while the biofilms formed on solid surfaces detached readily. (asm.org)
  • planktonic
  • In addition, the viable count of biofilm, but not planktonic phase ahyI mutants, was significantly lower that the parent or ahyR mutant. (nih.gov)
  • We recently discovered that quorum-sensing can control the balance between a planktonic or biofilm lifestyle, suggesting that modulation of this dispersal mechanism could be an effective therapeutic strategy. (grantome.com)
  • ii) define the significance of nuclease in mouse models of planktonic versus biofilm infection;and (iii) compare the host inflammatory response to nuclease in planktonic versus biofilm infection. (grantome.com)
  • Biologically, we would expect that planktonic bacterial cells would colonize any new surface, including the surface of an agar plate, while the specialized sessile cells of a biofilm community would have no such proclivity. (springer.com)
  • Cruz Olivo EA, Santos D, Elena de Lima M, Dos Santos VL, Sinisterra RD, Cortes ME (2016) Antibacterial effect of synthetic peptide LyeTxI and LyeTxI/betaCD association compound against planktonic and multispecies biofilms of periodontal pathogens. (springer.com)
  • Cells in the mature biofilms can return to the planktonic stage through detachment, thus completing the cycle of biofilm development ( 49 , 63 ). (asm.org)
  • pathogen
  • Aeromonas hydrophila is an opportunistic Gram-negative pathogen that readily attaches to stainless steel to produce a thin biofilm with a complex 3D structure covering 40-50% of the available surface and producing large microcolonies. (nih.gov)
  • maturation
  • Based on these findings, our central hypothesis is that control over extracellular nuclease activity is a critical determinant of biofilm maturation and dispersal. (grantome.com)
  • In the absence of the msaABCR operon, Atl is processed by proteases at a high rate, leading to increased cell death and a defect in biofilm maturation. (usm.edu)
  • dispersal
  • Unlike the previously described dispersal of cells that occurs after tower development, we found that the mechanism controlling this exodus event is dependent on the Sae regulatory system and independent of Agr. (nih.gov)
  • Bacterial signaling molecules that trigger the dispersal of old biofilms hold promise as possible therapeutic agents. (asm.org)
  • ii) determine whether nuclease activity is important for biofilm dispersal;and (iii) modulate biofilm integrity with controlled exposure to nuclease. (grantome.com)
  • iii) characterize the best candidates as dispersal agents in a biofilm infection model;and (iv) compare results to transposon mutants with increased nuclease activity. (grantome.com)
  • bacterium
  • Proteomic studies highlight outer-membrane proteins related to biofilm development in the marine bacterium Pseudoalteromonas sp. (bioinfor.com)
  • The aim of this study is to establish a novel high resolution tracking ability of a specific bacterium in multispecies biofilm. (springer.com)
  • The assay allows quantification of the ratio of a single bacterium within the biofilm, and its viable proportion. (springer.com)
  • proteins
  • Taken together, these results indicate that vps intergenic region genes encode proteins that are involved in biofilm matrix production and maintenance of biofilm structure and stability. (asm.org)
  • biomass
  • Furthermore, our data show that the wild-type biofilm biomass, as well as the material inside the domes, stains with a fucose-binding lectin. (ku.edu)
  • There has been an increasing interest and emphasis on the sessile bacterial lifestyle biofilms since it was discovered that the vast majority of the total microbial biomass exists as biofilms. (springer.com)
  • It has been shown that the development of these structures depends on several factors, including biomass growth rate, twitching motility, signaling molecules, and exopolysaccharide production ( 15 , 49 , 51 ). (asm.org)
  • Root Canals
  • Concerted efforts to study root canal biofilms have been made in the past decade, resulting in the publication of observational and experimental studies that detail the morphology and biology of these structures in infected root canals. (springer.com)
  • In addition, a pilot ex vivo test was conducted to examine the bactericidal effect of the plasma against saliva-derived biofilms cultivated in human root canals. (springer.com)
  • vitro
  • Fontana CR, Song X, Polymeri A, Goodson JM, Wang X, Soukos NS (2015) The effect of blue light on periodontal biofilm growth in vitro. (springer.com)
  • Guggenheim B, Giertsen E, Schupbach P, Shapiro S (2001) Validation of an in vitro biofilm model of supragingival plaque. (springer.com)
  • Guggenheim B, Gmur R, Galicia JC, Stathopoulou PG, Benakanakere MR, Meier A, Thurnheer T, Kinane DF (2009) In vitro modeling of host-parasite interactions: the 'subgingival' biofilm challenge of primary human epithelial cells. (springer.com)
  • Sanchez MC, Marin MJ, Figuero E, Llama-Palacios A, Leon R, Blanc V, Herrera D, Sanz M (2014) Quantitative real-time PCR combined with propidium monoazide for the selective quantification of viable periodontal pathogens in an in vitro subgingival biofilm model. (springer.com)
  • We report here an in vitro study of the antimicrobial effect of the room temperature plasma jet against monolayer Enterococcus faecalis biofilms on bovine dentins. (springer.com)
  • porous media
  • The multi-physical framework of biofilms in porous media is highly complex where the mechanical, chemical and biological aspects interacting at different scales are poorly understood and very partially controlled. (imft.fr)
  • The main objective of this thesis is to propose robust and reproducible experimental methodologies for the investigation of biofilms in porous media. (imft.fr)
  • To our knowledge, the scientific approach followed, along with the experimental apparatus, form the most complete methodology, at this time, for the study of biofilms in porous media. (imft.fr)
  • It is a challenge to upscale solute transport in porous media for multispecies bio-kinetic reactions because of incomplete mixing within the elementary volume and because biofilm growth can change porosity and affect pore-scale flow and diffusion. (utexas.edu)
  • mutants
  • Although the colony morphology of rbmDEF mutants was similar to that of the rugose variant, their biofilm structure and cell aggregation phenotypes were different than those of the rugose variant. (asm.org)
  • Bacillus
  • Branda SS, Chu F, Kearns DB, Losick R, Kolter R (2006) A major protein component of the Bacillus subtilis biofilm matrix. (springer.com)
  • A natural signal for biofilm disassembly in Bacillus subtilis is certain d -amino acids, which are incorporated into the peptidoglycan and trigger the release of the protein component of the matrix. (asm.org)
  • quorum
  • The regulation of biofilm development by quorum sensing in Aeromonas hydrophila. (nih.gov)
  • As A. hydrophila possesses an N-acylhomoserine lactone (AHL)-dependent quorum-sensing system based on the ahyRI locus, the presence of the AhyI protein and C4-HSL within the biofilm phase was first established by Western blot and AHL biosensor analysis respectively. (nih.gov)
  • These data support a role for AHL-dependent quorum sensing in A. hydrophila biofilm development. (nih.gov)
  • In this study, we characterized B. thailandensis biofilm development and sought to determine if quorum sensing (QS) contributes to this process. (ku.edu)
  • composition
  • In the present study, we examined early biofilm development and identified two new stages (designated "multiplication" and "exodus") that were associated with changes in matrix composition and a distinct reorganization of the cells as the biofilm matured. (nih.gov)
  • The described method is reproducible and of high resolution, and allows the examination of microbes' composition and viability within a biofilm structure. (springer.com)
  • grown
  • S. aureus UAMS-1 (wild-type) biofilms were grown in a BioFlux microfluidics system, and bright-field images were captured throughout an 18-h time course experiment. (nih.gov)
  • A single cell suspension of P. gingivalis was grown in broth and biofilm to identify the location of these events on side scatter and forward scatter. (springer.com)
  • multicellular
  • In addition, we revealed that the gene encoding the secreted staphylococcal nuclease was expressed in only a subpopulation of cells, consistent with a model in which biofilms exhibit multicellular characteristics, including the presence of specialized cells and a division of labor that imparts functional consequences to the remainder of the population. (nih.gov)
  • spatial distribution
  • Results provided insight into the extent and spatial distribution of biofilm within the GAC bed. (upc.edu)
  • An experimental workbench under controlled physical and biological conditions is proposed along with a validated 3D imaging protocol based on X-ray micro-tomography (XR MT) using a novel contrast agent (barium sulfate and agarose gel) to quantify the spatial distribution of the biofilm. (imft.fr)
  • detachment
  • The experimental workbench that we have designed enables to monitor in real-time the evolution of transport properties (effective permeability), O2 concentrations and biofilm detachment by spectrophotometry, all under controlled hydrodynamical conditions. (imft.fr)
  • Detachment of mature biofilms may occur as a result of nutritional and oxygen availability or the presence of other environmental stresses ( 11 , 22 , 49 , 56 , 59 ). (asm.org)
  • exopolysaccharide production
  • Using protein- and polysaccharide-specific stains, we have shown that d -amino acids inhibited the accumulation of the protein component of the matrix but had little effect on exopolysaccharide production and localization within the biofilm. (asm.org)
  • structures
  • In contrast to the existing model for tower development in S. aureus, the results of this study suggest the presence of a Sae-controlled nuclease-mediated exodus of biofilm cells that is required for the development of tower structures. (nih.gov)
  • An AHL-negative strain produced biofilms consisting of cell aggregates but lacking the matrix-filled dome structures. (ku.edu)
  • Of the three B. thailandensis QS systems, we show that QS-1 is required for proper biofilm development, since a btaR1 mutant, which is defective in QS-1 regulation, forms biofilms without these dome structures. (ku.edu)
  • Our work shows that B. thailandensis produces biofilms with unusual dome structures under flow conditions. (ku.edu)
  • Our findings suggest that these dome structures are filled with a QS-regulated, fucose-containing exopolysaccharide that may be involved in the resilience of B. thailandensis biofilms against changes in the nutritional environment. (ku.edu)
  • growth
  • Interestingly, after 6 h of growth, an exodus of the biofilm population that followed the transition of the biofilm to DNase I sensitivity was demonstrated. (nih.gov)
  • The biofilm mode of growth is predominant in nature (for e.g. the slimy matter forming on rocks at river bottoms, the viscous deposit in water pipes or even dental plaque) and confers a suitable environment for the development of the micro-organisms. (imft.fr)
  • In 1993, the American Society for Microbiology (ASM) recognized that the biofilm mode of growth was relevant to microbiology. (springer.com)
  • While the pore-scale subdomains involving significant biofilm growth and reaction are simulated using pore-scale equations, the other subdomains are simulated using continuum-scale equations to save computational time. (utexas.edu)
  • We present results for a simplified two-dimensional system, neglect advection, and use dual Monod kinetics for solute utilization and biofilm growth. (utexas.edu)
  • We conclude that the msaABCR operon plays a key role in maintaining the balance between autolysis and growth within the staphylococcal biofilm. (usm.edu)
  • cells
  • An effective strategy for S. aureus to survive in the host is to attach to a surface and develop into an encased community of cells called a biofilm. (grantome.com)
  • disruption
  • Furthermore, disruption of the gene encoding staphylococcal nuclease (nuc) abrogated this exodus event, causing hyperproliferation of the biofilm and disrupting normal tower development. (nih.gov)