Determined by flow cytometry determines cell cycle. Medical search. Frequent questions

Moreover, we found that knockdown of Annexin A2 significantly suppressed cell proliferation and invasion of NSCLCs cells. (jcancer.org)
Knockdown of Annexin A2 significantly suppressed cell proliferation and invasion of NSCLCs cells, and the biological effect of Annexin A2 on proliferation and invasion was mediated by the inhibition of p53, which in turn, promoted cell cycle progression and EMT. (jcancer.org)
In response to mito- pendence between the key mecha- gens, cell proliferation is triggered by nistic characteristics. (who.int)
H - Ras cell proliferation are also linked with haematopoietic cel s. (who.int)

Description: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. (thermofisher.com)

DeMarini, and Chapter 20, by Rice and cell death determine the size protein in several signal ing path- and Herceg). (who.int)

Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells. (thermofisher.com)
It could be divided into two categories, small cell lung cancers (SCLCs) and non-small cell lung cancers (NSCLCs). (jcancer.org)

Applications Reported: PK136 has been reported for use in flow cytometric analysis, depletion of NK cells, and in vitro functional studies. (thermofisher.com)
Mechanistically, our studies showed that knockdown of Annexin A2 increased the expression of p53, which in turn, induced cell cycle G2 arrest and inhibited epithelial-to-mesenchymal transition (EMT). (jcancer.org)

Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. (thermofisher.com)
However, the exact function and mechanism of Annexin A2 in tumor progression of NSCLCs need to be further determined. (jcancer.org)

In the present study, we found that Annexin A2 was overexpressed in human NSCLCs cell lines and NSCLCs tissues. (jcancer.org)

Applications Tested: The PK136 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes and can be used at less than or equal to 0.5 µg per test. (thermofisher.com)

0.2 × 10 9 /L), the inflammatory response may be muted, and the usual inflammatory findings of leukocytosis or white blood cells in the urine or at the site of infection may not occur. (msdmanuals.com)

Here we report that treatment of the 5637 human bladder carcinoma cells with 2% cis-UCA induces both apoptotic and necrotic cell death. (biomedcentral.com)
Treatment of 5637 bladder carcinoma cells with 2% cis-UCA induced a combination of apoptotic and necrotic cell death. (biomedcentral.com)
Within the scope of the protodynamic therapy concept, our study suggests a novel mechanism for cis-UCA-induced inhibition of cancer cell survival, based upon compromised signalling and metabolic functions and cell cycle arrest leading to apoptotic and necrotic cell death. (biomedcentral.com)

Phosphatase activity in cis-UCA-treated cells was determined by assay kits measuring absorbance resulting from the dephosphorylation of an artificial substrate. (biomedcentral.com)

In addition, metabolic activity of the 5637 cells is rapidly impaired, and the cells arrest in cell cycle in response to cis-UCA. (biomedcentral.com)
In addition, metabolic activity of the 5637 cells was rapidly impaired, and cell cycle arrest was induced at 1-3% cis-UCA. (biomedcentral.com)
Additionally, G15 decreased cyclin D1, cyclin B1, CDK1, and p-CDK1 expression, resulting in cell cycle arrest in the G2/M phase via a down-regulated phosphorylation of Erk1/2 and Akt compared with estrogen alone. (researchsquare.com)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

Cell death was assessed by measuring caspase-3 activity, mitochondrial membrane polarization, formation and release of cytoplasmic histone-associated DNA fragments, and cellular permeabilization. (biomedcentral.com)

The current study was aimed at characterizing the molecular mechanisms underlying cis-UCA-mediated cytotoxicity in cultured cancer cells. (biomedcentral.com)
In the current study, we aimed at characterizing the molecular mechanisms underlying cis-UCA-mediated cytotoxicity to cultured cancer cells. (biomedcentral.com)

The use of cis-UCA as an anticancer agent against non-muscle-invasive bladder cancer has previously been evaluated in cultured moderately and poorly differentiated human transitional bladder cancer cells using a short term (up to 2 h) pulse treatment model in vitro , which mimics the clinical intravesical chemotherapy regimen [ 3 ]. (biomedcentral.com)

Furthermore, MEK inhibitor and PI3K inhibitor decreased the expression of cyclin D1, cyclin B1, CDK1, and p-CDK1, and repressed estrogen-induced and G1-driven promotion of cell growth. (researchsquare.com)

Our analyses suggest that CAMK2N1 plays a tumor suppressive role in prostate cancer cells. (oncotarget.com)

Cell viability and metabolic activity were monitored by colorimetric assays. (biomedcentral.com)
During lactation, maintaining a high proliferative activity in goat mammary epithelial cells (GMECs) is significant to improve the yield and composition of goat milk. (researchsquare.com)