• Samaridou E, Heyes J, Lutwyche P. Lipid nanoparticles for nucleic acid delivery: Current perspectives [J]. Advanced Drug Delivery Reviews , 2020. (cd-bioparticles.net)
  • Conformationally rigid nucleoside probes help understand the role of sugar pucker and nucleobase orientation in the thrombin-binding aptamer. (ub.es)
  • The use of conformationally rigid nucleoside probes to study the role of sugar pucker and nucleobase orientation in the thrombin binding aptamer. (irbbarcelona.org)
  • Specific loop modifications of the thrombin-binding aptamer trigger the formation of parallel structures. (irbbarcelona.org)
  • the creation of a large number of compounds (usually proteins or peptides) through technologies such as phage display . (genomicglossaries.com)
  • Here we discuss two approaches currently used to investigate the global dynamics of phosphorylation based on the isolation of phosphorylated proteins or peptides. (acs.org)
  • Together with advanced preparation technologies for various liposomes, scientists in CD Bioparticles are proficient in producing LNPs for various types of drug loading, especially for nucleic acid drug (eg siRNA, miRNA, mRNA and plasmid DNA, et al. (cd-bioparticles.net)
  • acetylation acetyl-CoA A molecule that participates in many biochemical reactions in protein, carbohydrate, and lipid metabolism, notably the citric acid cycle. (wikipedia.org)
  • In this study, using a combination of the bioinformatics design and molecular genetics, the protein A signal peptide from Staphylococcus aureus was modified, redesigned and then fused to the mature hGH coding region. (biomedcentral.com)
  • Since the native signal protein peptide of S. aureus protein A was not able to deliver hGH to the extracellular space, it was modified using bioinformatics tools and fused to the n-terminal region of hGh to show that the redesigned signal peptide was functional. (biomedcentral.com)
  • They are isolated via SELEX (Systematic Evolution of Ligands by Exponential Enrichment), an evolutionary process that involves iterative rounds of selection and amplification before sequencing and aptamer characterization. (genomicglossaries.com)
  • Aptamers generated in vitro can be systematically isolated from a large pool of 1012-1015 synthetic oligo sequences using an iterative process called systematic evolution of ligands by exponential enrichment (SELEX). (encyclopedia.pub)
  • This paper critically reviews the progression of pesticide aptasensors throughout the development process, including the selection, characterization and modification of aptamers, the conceptual frameworks of integrating aptamers and biosensors, the ASSURED (affordable, sensitive, specific, user-friendly, rapid and robust, equipment-free and deliverable to end users) criteria of different platforms and the future outlook. (encyclopedia.pub)
  • The use of tailor-designed biomolecules, such as aptamers and molecularly imprinted polymers, is reviewed. (scirp.org)
  • Aptamers are short nucleic acid sequences capable of specific, high-affinity molecular binding. (genomicglossaries.com)
  • somewhat similar sequences of about 110 amino acids each. (wikipedia.org)
  • We analyzed certified reference materials containing different amounts of genetically modified DNA by using a detection method which combines the nanoparticle-enhanced surface plasmon resonance imaging (SPRI) biosensing to the peptide nucleic acids (PNAs) improved selectivity and sensitivity in targeting complementary DNA sequences. (cnr.it)
  • Labeling of Trypanosoma brucei cell cultures with 50% uniformly 13C-labeled glucose demonstrated incorporation of glucose-derived carbon into 187 of 588 putatively identified metabolites in diverse pathways including carbohydrate, nucleotide, lipid, and amino acid metabolism. (acs.org)
  • The use of ionizable lipid resulted in the high encapsulation efficiency of nucleic acids because of the electrostatic interactions. (cd-bioparticles.net)
  • Aptamer Bioinformatics. (genomicglossaries.com)
  • Recent advances in peptide-based (bottom-up) quantitative proteomics and bioinformatics have opened unprecedented opportunities for extensive investigation of cellular proteomes and their dynamics. (acs.org)
  • Bioinformatic algorithms allowing predicting of biomolecular folding for proteins, peptides, and RNAs, even though sometimes successful, have all their limitations. (biosyn.com)
  • RNA molecules in solution may adopt secondary structures that are only partially determined by thermodynamics since RNA molecules can undergo conformational changes during interaction with other RNAs, RNA binding proteins or RNA binding peptides. (biosyn.com)
  • They include coding mRNAs and non-coding (nc) RNAs among them antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), micro-RNAs (miRNAs), small activating RNAs (saRNAs), RNA aptamers and RNA guides. (frontiersin.org)
  • There are currently several products containing RNA on the market, and many are under development, among them mRNA, antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), small activating RNAs (saRNAs), micro-RNAs (mi-RNAs), RNA aptamers and RNA guides. (frontiersin.org)
  • It utilizes aptamers, short single-stranded DNAs or RNAs, as pesticide recognition elements to integrate with various innovative biosensing technologies for specific and sensitive detection of pesticide residues. (encyclopedia.pub)
  • Molecular basis of substrate-induced permeation by an amino acid antiporter. (ub.es)
  • As aptamers are genetic in nature, bioinformatic approaches have been used to improve both aptamers and their selection. (genomicglossaries.com)
  • Design of peptide-membrane interactions to modulate single-file water transport through modified gramicidin channels. (ub.es)
  • Molecular modeling indicates the three-dimensional structure of the peptide spine of aggregates is essentially constituted by extended β-sheet motifs stabilized by hydrogen bonds and hydrophobic interactions. (cnr.it)
  • Here, we show that mammalian cells critically depended on the editing of mitochondrial threonyl-tRNA synthetase (mtThrRS, encoded by ), disruption of which accumulated Ser-tRNA and generated a large abundance of Thr-to-Ser misincorporated peptides in vivo. (cnrs.fr)
  • In this work, a novel, simple and reproducible poly(l-proline)/functionalized multi-walled carbon nanotube composite on glassy carbon electrode (poly(PRO)-MWCNTs/GCE) was developed as an electrochemical sensor for the simultaneous determination of gallic acid (GA), theophylline (TP) and caffeine (CAF) by differential pulse voltammetry (DPV). (bvsalud.org)
  • The antimicrobial wasp Polybia-MP1 peptide selectively kills bacterial cells! (biosyn.com)
  • On a molecular level, lead can alter or interfere with the activity of proteins and nucleic acids, ultimately resulting in changes in cell metabolism and physiology leading to pathologic states. (edu.pl)
  • Molecular Therapy - Nucleic Acids , 33 , 543-544. (au.dk)
  • C. La Rosa, F. Pariente, L. Hernandez and E. Lorenzo, "Determination of Organophosphorus and Carbamic Pes- ticides with an Acetylcholinesterase Amperometric Bio-sensor Using 4-Aminophenyl Acetate as Substrate," Analytica Chimica Acta, Vol. 295, No. 3, 1994, pp. 273-282. (scirp.org)
  • An aptamer requires either single-stranded DNA or RNA with a length of approximately 15-100 nucleotides to maintain its specific recognition and discrimination of the cognate target in a heterogeneous milieu [ 1 ] . (encyclopedia.pub)
  • Naturally, aptamers can be found in living organisms in the form of riboswitches, which function by binding with their ligands for subsequent regulation of specific gene expression [ 3 ] . (encyclopedia.pub)
  • Nucleic acids research. (elsevierpure.com)
  • This research provides critical new insights regarding SERS signal variation and offers a simple methodology for reliable determination of the SERS enhancement factors. (acs.org)
  • One of the most promising technologies is an aptamer-based biosensor or so-called aptasensor. (encyclopedia.pub)
  • 08:00 00571 Use of Magnetic Microbeads for Miniaturized Sample Extraction in Mass Spectral Analysis of Proteins and Peptides * $ Yeung K.K.C. , Jurcic K., Wang J., Thompson A., Strybosch K. (csc2013.ca)
  • Since the natural growth hormone signal peptide is of eukaryotic origin and is not detectable by any of the Escherichia coli secretory systems, including Sec and Tat, and is therefore unable to secrete hGH in the prokaryotic systems, designing a new and efficient signal peptide is essential to direct hGh to the extracellular space. (biomedcentral.com)
  • The peptide conjugate Gd-DOTA-L(6)-(2Nal)(2) is able to self-assemble in long fibrillary nanostructures in water solution (up to 1.0 mg/mL). (cnr.it)
  • This paper reports a validated Liquid Chromatography with tandem mass spectrometry (LC-MS/MS) method to simultaneously quantify TRP, L-kynurenine (KYN), xanthurenic acid (XA), 3-hydroxykynurenine (3OHKYN), kynurenic acid (KA), 3-hydroxyanthranilic acid (3OHAA), anthranilic acid (AA), 5-hydroxytryptamine (serotonin, 5HT) and tryptamine (TRYP) in Dulbecco's Modified Eagle and Eagle's Minimum Essential Media (DMEM and EMEM, respectively). (bvsalud.org)