• The Centers for Disease Control and Prevention developed, optimized, and rapidly qualified an enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G (IgG) antibodies to Bacillus anthracis protective antigen (PA) in human serum. (cdc.gov)
  • Variations in antigen preparation and purity, assay methods, and endpoint determination between laboratories and the absence of a suitable standard reference serum compound this problem. (cdc.gov)
  • The Centers for Disease Control and Prevention (CDC) had, before the attacks, instituted the development of anthrax serologic assays-particularly enzyme-linked immunosorbent assays (ELISAs)-for use in anthrax vaccine studies in humans and to provide a standard human reference serum. (cdc.gov)
  • In response to the anthrax emergency of 2001, we report the accelerated development and qualification of a quantitative ELISA for detection of anti-protective antigen (PA) specific immunoglobulin (Ig) G in human serum and the development of a competitive inhibition assay to enhance diagnostic specificity. (cdc.gov)
  • Determination of MIF serum levels were performed by enzyme-linked immunosorbent assay and MIF ELISA Kit. (hindawi.com)
  • The IMMUNO-TEK Human IgA ELISA Kit is a rapid, easy to use enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human IgA in serum, plasma, cell culture supernatants or other biological fluids. (zeptometrix.com)
  • The plasma of all the samples were subjected to kaolin clotting time and the serum of forty-three (43) of the samples were run on an enzyme-linked immunosorbent assay (ELISA) for the semi quantitative determination of IgG and IgM anticardiolipin antibodies. (ispub.com)
  • This is an enzyme-linked immunosorbent assay (ELISA) for the semiquatitative determination of IgG and IgM anticardiolipin antibodies in human serum or plasma. (ispub.com)
  • The Wampole Laboratories (Wampole) H. pylori IgG Enzyme-Linked Immunosorbent Assays (ELISA) is intended for the detection and qualitative determination of IgG antibodies to Helicobacter pylori in human serum. (cdc.gov)
  • Exposure to polycyclic aromatic hydrocarbons (PAHs) in foundry workers has been evaluated by determination of benzo(a)pyrene-serum albumin adducts and urinary 1-hydroxypyrene. (bmj.com)
  • Quantitative determination of serum 25-OHD was performed using an enzyme-linked immunosorbent assay (ELISA). (scholarsresearchlibrary.com)
  • Human bocavirus (HBoV) is a recently discovered parvovirus associated with mild to severe lower respiratory tract infections in children, the aim of the work was determination of human bocavirus in nasopharyngeal aspirate (NPA) of infants by qualitative PCR and determination of acute human bocavirus infection by estimation of immunoglobulin M (IgM) antibodies in serum by enzyme linked immunosorbent assay. (scienceopen.com)
  • The IpaD ELISA for serum immunoglobulins G and A correlated well with the water-extract ELISA in that monkeys infected with S. flexneri or S. sonnei responded with high serum antibody titers in both assays. (ku.edu)
  • Mycoplasma pneumoniae IgG ELISA Kit is designed for the detection and quantitative determination of specific IgG antibodies against mycoplasma pneumoniae in serum and plasma. (medicregister.com)
  • In conclusion, IDT and serum IgE determination to glatiramer acetate seem useful for identifying allergic reactions among the common local reactions induced by this drug. (jiaci.org)
  • Serum: Store fresh serum samples immediately after collection at -20°C or lower until assayed. (tecomedical.com)
  • The Perch (Perciformes) Vitellogenin ELISA Kit is a sensitive sandwich enzyme linked immunosorbent assay for the quantitative determination of vitellogenin in serum and mucus of perciformes according to EC regulation Nr. 440/2008 (REACH) from July 10th 2015/ Document D039048/03. (tecomedical.com)
  • The CeliAK EmA human is a quantitative immunoassay for the determination of IgA antibodies against endomysial antigens (EmA) in human serum. (medipan.de)
  • More advanced diagnostic tests such as enzyme linked immunosorbent assays (ELISAs) do possess this capability but have similar drawbacks to microscopy, requiring expensive equipment, long incubation times, and trained staff. (hindawi.com)
  • Two PEDV spike (S) glycoprotein-based indirect enzyme-linked immunosorbent assays (ELISAs) were developed using G2b PEDV-Pintung 52 (PEDV-PT) trimeric full-length S and truncated S 1-501 proteins derived from the human embryonic kidney (HEK)-293 cell expression system. (biomedcentral.com)
  • Enzyme linked immunosorbent assays (ELISA) rely on the ability of biological materials, (i.e. antigens) to adsorb to plastic surfaces such as polystyrene (solid phase). (cdc.gov)
  • To overcome the lack of specificity for individual antigens in the water-extract enzyme-linked immunosorbent assay (ELISA), the ipaD gene from S. flexneri has been cloned, expressed to a high level, and purified for use in a new ELISA for the determination of the levels of antibody against IpaD in monkeys and humans challenged with shigellae. (ku.edu)
  • The IpaD assay required less antigen per well, had much lower background levels, and did not require correction with antigens from an avirulent organism. (ku.edu)
  • It has been shown conclusively that Beta2GP1 is indeed the antigen to which many anticardiolipin antibody patients are actually binding and furthermore showed that the phospholipid merely serves to link the Beta2GP1 to the solid phase. (rapidtest.com)
  • After adding enzyme conjugate, it binds to the antibody-antigen complex. (rapidtest.com)
  • This event necessitated the simultaneous development and application of qualified laboratory assays-including serologic assays-to evaluate patients suspected of having anthrax. (cdc.gov)
  • The major obstacle to serologic analysis of human anthrax has been the lack of assay standardization. (cdc.gov)
  • The assays were applied to diagnosis of cutaneous and inhalational anthrax to evaluate serologic responses in persons considered at risk from anthrax spore exposure and enhance anthrax serologic tests with standardized techniques for distribution to public health and clinical laboratories. (cdc.gov)
  • Determination of IgG and IgA to deamidated gliadin by ELISA as well as the detection of Human endomysial autoantigens (EmA) IgA has been considered as the main serological parameters for celiac disease so far. (medipan.de)
  • A secondary antibody conjugated with the enzyme peroxidase detects the generated immune complex. (medipan.de)
  • A reliable serological assay for evaluating the PEDV-specific humoral and mucosal immune response is important for disease survey, monitoring the efficacy of immunization, and designing strategies for the prevention and control of PED. (biomedcentral.com)
  • 1997). Commercial Assays for the Serological Diagnosis of Dengue Infection. (ncnsd.org)
  • Sensitivity from equilibrium and from non-equilibrium assays was 41 and 2.3 ng/ml respectively. (alr-journal.org)
  • Kit includes 96-well microplate, 12 mL detector antibody, 7 mL human IgA standard, 100 mL assay diluent, 125 mL plate wash buffer, 12 mL substrate, 12 mL stop solution, 10 plate sealers, and one plastic storage bag. (zeptometrix.com)
  • A two step competitive assay was developed in which VTG could be quantitated by its capacity to inhibit the binding of antibody to the VTG previously adsorbed onto a solid phase. (alr-journal.org)
  • Thermal stability of the helical structure of type IV collagen within basement membranes in situ: determination with a conformation-dependent monoclonal antibody. (rupress.org)
  • We previously observed by competition enzyme-linked immunosorbent assay that, in neutral solution, the helical epitope to which this antibody binds undergoes thermal denaturation over the range of 37-40 degrees C. In the present study, we have reacted unfixed cryostat tissue sections with this antibody at successively higher temperatures. (rupress.org)
  • The enzyme-linked immunosorbent assay (ELISA) is the most popular in many clinical laboratories. (medscape.com)
  • These revised guidelines were developed by CDC for laboratories performing lymphocyte immunophenotyping assays in human immunodeficiency virus-infected persons. (cdc.gov)
  • Although flow cytometric immunophenotyping is a highly complex technology, methodology for performing CD4+ T-cell determinations has become more standardized between laboratories. (cdc.gov)
  • 1997 Revised Guidelines for Performing CD4+ T-Cell Determinations. (cdc.gov)
  • Enzyme-linked immunosorbent assay was used for measuring collagen type I and III production. (thieme-connect.com)
  • An investigation was conducted by applying the enzyme linked immunosorbent assay method to the determination of the contents and distribution of Type-I and Type-III collagen in female Wistar-rats who had been exposed to either quartz (14808607) dust or welding fume dust. (cdc.gov)
  • It has been suggested that endogenous factors might contribute to the rapid progression of periodontal breakdown such as inappropriate regulation of enzymes, lipid mediators, collagen biosynthesis or T cell immunodeficiency. (bvsalud.org)
  • This assay is intended for use as an aid in the diagnosis of H. pylori infection in persons with gastrointestinal symptoms. (cdc.gov)
  • The results of the assay are to be used as an aid in the diagnosis of certain autoimmune disease thrombotic disorders, anti-phospholipid syndrome, SLE or lupus-like disorders. (rapidtest.com)
  • Enzyme activity in the wells is then quantified using tetramethyl benzidine as a substrate. (zeptometrix.com)
  • Excess enzyme conjugate is washed off, and TMB Chromogenic substrate is added. (rapidtest.com)
  • Because a limited amount of EPO products was available, we were restricted to the common assays used for quality control: high performance size exclusion chromatography (HP-SEC) to assess monomer and aggregate content, ELISA to determine EPO protein content, SDS-PAGE under non-reducing conditions to detect possible fragmentation and covalent protein aggregates, capillary zone electrophoresis (CZE) for isoform profiling and a normocythemic mouse assay to test for potency. (springer.com)
  • Benzo(a)pyrene binding to albumin and 1-hydroxypyrene were quantitatively measured by enzyme linked immunosorbent assay (ELISA) and reverse phase high performance liquid chromatography (HPLC), respectively. (bmj.com)
  • Aldo-keto reductase family 1, member B1 (AKR1B1), also known as aldose reductase, is an enzyme that is encoded by the AKR1B1 gene in humans. (wikipedia.org)
  • All samples were run using the conditions indicated in TaqMan Gene Expression Assay protocol. (hindawi.com)
  • The way it binds NADPH differs from other nucleotide adenine dinucleotide-dependent enzymes. (wikipedia.org)
  • The assay contains ready-to-use reagents and takes less than two hours to perform. (zeptometrix.com)
  • In September 2019, Gyros Protein Technologies AB, a provider of automated nanoliter-scale immunoassays, peptide synthesizers, and reagents, introduced Gyrolab p24 Kit for lentivirus titer determination in lentivirus vector manufacturing (2). (biopharminternational.com)
  • The HMW agents that were considered most relevant were flour dusts, enzymes (both plant and animal derived), gums, foods and tobacco, rubber-derived proteins, animal- and insect-derived allergens, and fish/ seafood-derived allergens ( Table 1 ). (cdc.gov)
  • Enzymes are proteins used as biocatalysts to reduce or replace the use of chemi- cals in a variety of processes.9 As catalysts, enzymes are used in a variety of indus- tries, including cleaning, food processing, animal feed, fuel alcohol, textile, paper, and pharmaceuticals. (cdc.gov)
  • The CDC guidelines concerning CD4+ T-cell determinations (33) were first published in the MMWR in 1992 to provide laboratorians with the most complete information about how to measure CD4+ T-lymphocytes in blood from HIV-infected persons by using flow cytometry. (cdc.gov)
  • The household questionnaire and phlebotomy files may be linked to the laboratory data file using the unique survey participant identifier SEQN. (cdc.gov)
  • Its features include automatic performance test with optional test plate, two point calibrations using stored curve or master curve, multiple assays per plate and PC connection for data output to laboratory information management systems. (medicregister.com)
  • This test is a determination of total beta-hCG levels. (medscape.com)
  • Upregulation of NOS was assessed by measuring enzyme levels in lung homogenates by Western blot analysis and enhancement of the COX-related pathway was judged from perfusate concentrations of 6‐ketoprostaglandin F 1α . (ersjournals.com)
  • This product is not intended to replace the manufacturer's controls provided with the assay. (zeptometrix.com)
  • Le test enzymatique immuno-adsorbant (ELISA) développé dans cette étude a été validé physiologiquement en détectant des variations de VTG chez des crevettes femelles nourries avec des extraits de calmar. (alr-journal.org)
  • This publication describes the synthesis, silane functionalization and use of magnetic Iron nanoparticles in combination with enzyme-linked immunosorbent assay (ELISA) to facilitate the hybrid technique of 'magnetic nanoparticles - ELISA' for use in the capture of progesterone in numerous types of milk. (syrris.com)
  • Plasmodium aldolase is a malaria biomarker measured using enzyme linked immunosorbent assay (ELISA) techniques. (hindawi.com)
  • Microplate Stacker is a higher capacity device that interfaces to a variety of peripheral microplate assay products. (medicregister.com)
  • Estimates of within-assay and between-assay variabilities of standard curves were 4.2 and 9.6% respectively. (alr-journal.org)
  • Un essai compétitif en deux étapes a été développé par lequel la VTG peut être quantifiée en fonction de sa capacité d'inhiber la liaison de l'anticorps à la VTG préalablement adsorbée à une phase solide. (alr-journal.org)
  • Linking to a non-federal website does not constitute an endorsement by CDC or any of its employees of the sponsors or the information and products presented on the website. (cdc.gov)
  • Citric acid-Na 2 HPO 4 -buffered stock solution for use in the determination of fecal urease activity. (sigmaaldrich.com)
  • When an assay on a range of mixture of normal and patient's plasma is done, the presence of Lupus anticoagulant deficiency is indicated by the different patterns of response obtained. (ispub.com)
  • Water samples were assayed for potential respiratory viruses (adenoviruses and enteroviruses), but none were detected. (cdc.gov)
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