• This mild nonionic detergent can be used to solubilize receptors and permeabilize cellular and nuclear membranes. (yunbangpharm.com)
  • Nonionic detergents contain uncharged hydrophilic head groups that consist of either polyoxyethylene moieties as in BRIJ, PEG-sorbitan units as in TWEEN, or glycosidic groups as in octyl-ß-D-glucoside, dodecyl-ß-D-maltoside or digitonin. (serva.de)
  • Photosystem I (PS I) reaction center complexes isolated from the thermophilic cyanobacterium Synechococcus elongatus with nonionic detergents, digitonin or sucrose monolaurate, contained eight small subunit polypeptides. (elsevierpure.com)
  • Digitonin is a steroidal saponin (saraponin) obtained from the foxglove plant Digitalis purpurea. (wikipedia.org)
  • One more detergent utilised in cryoEM structure determination is digitonin (an amphipathic steroidal saponin) [99]. (lrrk2inhibitor.com)
  • 1. Prepare dead cells by 10 minutes incubation in 0.1% saponin or 0.1-0.5% digitonin or by 30 minutes incubation in 70% ethanol. (dojindo.com)
  • Moreover, LAM gene deletion reduced yeast resistance to Rg3 and digitonin, another saponin with glycosylated aglycon in the C-3 position. (bvsalud.org)
  • To extract membrane proteins, detergents that solubilize membrane proteins (membrane protein solubilizers) are used. (fujifilm.com)
  • Five PS I complexes isolated with an anionic detergent, sodium dodecylsulfate, contained zero to four small subunits but were all depleted of the psaF polypeptide. (elsevierpure.com)
  • Treatment with a cationic detergent, cetyltrimethylammonium bromide, resulted in depletion of five small subunits including the psaF gene product. (elsevierpure.com)
  • Compared to ionic detergents, they usually do not denature proteins and are therefore frequently used for the isolation of biologically active membrane proteins. (serva.de)
  • B ) Size exclusion profile of purified wild-type (WT) ABCA4 and the E1087Q/E2096Q mutant (EQ). ( C ) Basal ATPase activity measured in 0.06% digitonin at 28°C. Data points represent the means and standard deviations (SDs) of three measurements. (elifesciences.org)
  • It might be hard to distinguish the protein particle from a detergent by means of a damaging EM stain, as identified in the study of citrate transporter CitS in DDM and DM [96]. (lrrk2inhibitor.com)
  • Given this, option NMR studies on IMPs call for detergent micelles to become as compact (little) as you can but still adequately mimic the membrane environment [103]. (lrrk2inhibitor.com)
  • The lysate was centrifuged (8000 g , 30 min) at 4°C and the membrane fraction containing luciferase was pelleted by ultracentrifugation (150 000 g , 90 min) at 4°C. To select the conditions for luciferase solubilization, the membranes were suspended in 50 mM HEPES-Na buffer (pH 8.0) containing 500 mM NaCl, 20% glycerol (buffer A), and various detergents at 4°C overnight ( Fig. 1 ). (springer.com)
  • The detergent-solubilized membrane fraction was loaded onto a column packed with the TALON sorbent for metal affinity chromatography equilibrated with buffer A with 0.02% DDM, and the column was washed with the same buffer. (springer.com)
  • Triton X-100 is the most commonly used detergent, especially for membrane protein solubilization. (sigmaaldrich.com)
  • The SUIT protocol SUIT-010 was designed as a test to evaluate the optimum digitonin concentration to permeabilize the cell plasma membrane. (mitoglobal.org)
  • A higher level of plasma membrane glucan synthase activities was also measured in detergent extracts of membrane fractions from the transgenic line, while no activation of Golgi-bound glycan synthases was detected. (edu.au)
  • Enzymatic hydrolysis and methylation analysis were performed on the products synthesized in vitro by the plasma membrane enzymes from the wild-type and transgenic lines extracted with digitonin and incubated with radioactive UDP-glucose. (edu.au)
  • Treatment of liver membranes with digitonin also caused a selective loss of hormone re- sponse. (nih.gov)
  • Digitonin is a mild detergent that permeabilizes plasma membranes selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations. (mitoglobal.org)
  • Fractionation of mitochondrial membranes extracted in detergent revealed components between 11 and 25kDa exhibiting ADP/Ca2+-sensitive labelling. (ucl.ac.uk)
  • The mitochondrial protein immunoprecipitation (IP) kit also provides choices of detergents, N -dodecyl-β-D-maltoside, Triton X-100, and digitonin, to achieve different stringency conditions for protein-protein interaction studies. (sigmaaldrich.com)
  • Digitonin is a natural product and thus the effective concentration has to be determined by titrations for every batch. (mitoglobal.org)
  • incubation of ghosts of fat cells with digitonin resulted in loss of response of the adenyl cyclase system to glucagon, adreno- corticotropin, secretin, and epinephrine at very low concen- trations of the detergent. (nih.gov)