Detection probes nucleic acid hybridization. Medical search. Frequent questions

A method is described for visualizing three nucleic acid sequences simultaneously by in situ hybridization using a new blue immunofluorescent label, amino methyl coumarin acetic acid (AMCA), in combination with green and red fluorescing FITC and TRITC. (nih.gov)
Three-color in situ hybridization was applied to the study of numerical chromosome abnormalities as occur in human solid tumors. (nih.gov)
2017). Detection and discrimination of biofilm populations using locked nucleic acid/2'-O-methyl-RNA fluorescence in situ hybridization (LNA/2'OMe-FISH) . (up.pt)
2016). Application of locked nucleic acid-based probes in fluorescence in situ hybridization . (up.pt)
2015). Erratum to: Mismatch discrimination in fluorescent in situ hybridization using different types of nucleic acids (Applied Microbiology and Biotechnology, 10.1007/s00253-015-6389-4) . (up.pt)
Peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay for specific detection of mycobacterium immunogenum and DNA-FISH assay for analysis of pseudomonads in metalworking fluid s and sputum. (cdc.gov)
Here, we report for the first time a species-specific peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method for Mycobacterium immunogenum, a non-tuberculous Mycobacterium species prevalent in MWF and implicated in occupational lung disease hypersensitivity pneumonitis and pseudo-outbreaks. (cdc.gov)
Although numerous methods are available for gaining information about miRNA signatures, in situ hybridization (ISH) assay is a powerful tool that allows direct assessment of malignant cells. (biogenex.com)
BioGenex is pleased to launch two Super Sensitive TM Nucleic Acid miRNA in situ hybridization (SSNA miRNA ISH) probes and automated systems for differentiation of wild type and BRCA mutated breast cancer. (biogenex.com)
The in situ experimental conditions for hybridization were optimized for both manual and automated systems. (biogenex.com)
We here present a micrometer-scale implementation of fluorescence in situ hybridization that we term μFISH. (springer.com)
A key milestone in ISH-based techniques was fluorescence in situ hybridization (FISH) (Bauman et al. (springer.com)
Therefore, we analyzed the potential of fluorescence in situ hybridization (FISH) for diagnosing WD, using a Tropheryma (T.) whipplei -specific probe. (frontiersin.org)
In Situ Hybridization (ISH) is a powerful technique that allows you to identify and localize nucleic acid targets in formalin-fixed tissue sections and cells. (ahdiagnostics.dk)
Flow cytometry in combination with fluorescent in situ hybridization (flow-FISH) is a powerful technique that can be utilized to rapidly detect nucleic acids at single-cell resolution without the need for homogenization or nucleic acid extraction. (nih.gov)
Cy3 can also be used to label DNA oligos for use as hybridization probes in other applications, such as Fluorescent In-Situ Hybridization (FISH). (genelink.com)
This Double Labeling of Oligonucleotide Probes for Fluorescence In Situ Hybridization (DOPE-FISH 0 strategy may provide an effective solution to the problem of low signal intensity, which is commonly observed when using corresponding singly-labeled FISH probes for microbe identification. (genelink.com)
As an added benefit, Cy3-doubly labeled probes were shown to increase the in situ accessibility of rRNA targets sites in microbes, which allows for greater probe design flexibility. (genelink.com)
The official U.S. patent, named "Nucleic acid beacons for in situ fluorescence hybridization and chip technologies," encompasses a concept for the development and use of new DNA probes for in vitro diagnostics and paves the way for the establishment of novel, rapid and convenient pathogen detection procedures in the U.S. market. (rapidmicromethods.com)
In situ hybridization with a blotin-labeled DNA probe was used to detect wheatrye translocations. (illinois.edu)
AMPIVIEW™ EGFR (AS) Dig RNA Probes are digoxigenin-labeled RNA probes targeting EGFR nucleic acid (DNA/RNA or RNA) for in situ hybridization in tissues and cells. (enzolifesciences.com)
We studied the distribution of bovine respiratory syncytial virus (BRSV) RNA in lungs of experimentally inoculated lambs by in situ hybridization at different times postinoculation. (nebraska.edu)
The probe used for in situ hybridization was prepared by reverse transcription of BRSV RNA, followed by polymerase chain reaction (PCR) amplification of the cDNA. (nebraska.edu)

Hybridization between the capture probe and the target DNA is achieved by melting and annealing complementary target DNA sequences to their capture probes located on the microspheres. (wikipedia.org)
After washing to remove non-specific binding between sequences, only strongly paired probe-targets will remain hybridized. (wikipedia.org)
Detection of the rRNA amplification product sequences is achieved using nucleic acid hybridization (HPA). (cdc.gov)
An RLU measurement less than the Cutoff Value indicates the absence of the specific HPV DNA sequences tested or HPV DNA levels below the detection limit of the assay. (cdc.gov)
The second approach, which attempted to detect AIDS virus-related nucleic acid sequences using dot blot hybridization analysis of the vaccine with an AIDS virus deoxyribonucleic acid (DNA) probe, was done at MSD using as a positive control infected cellular (ribonucleic acid) RNA preparations provided by CDC. (cdc.gov)
Detection of human papillomavirus gene sequences in cell lines derived from laryngeal tumors. (adventube.ro)
A biosynthetic precursor of collagen containing additional amino acid sequences at the amino-terminal and carboxyl-terminal ends of the polypeptide chains. (lookformedical.com)
The detection and quantitation of specific nucleic acid (NA) sequences continue to grow in importance. (dcu.ie)
6). Detection of specific nucleic acid sequences by hybridization a. mini-prep of plasmid DNA b. (ntu.edu.tw)
Molecular diagnostics are used to asses an individual's health condition at the molecular level, analyzing specific genetic sequences in DNA, ribonucleic acid (RNA), or the material or protein that is encoded in these genetic sequences, says Jiang. (medicaldesignbriefs.com)
Primers for RT-LAMP or RT-RPA can be designed for specific viral RNA sequences, and the amplification methods are used before the Cas enzyme detection step, to multiply detection signals from the specific nucleic acid sequences. (nature.com)
High-risk HPV DNA sequences are either absent or the HPV solution DNA levels are below the detection limit of the assay. (medscape.com)

2015). Towards fluorescence in vivo hybridization (FIVH) detection of H. pylori in gastric mucosa using advanced LNA probes . (up.pt)
Advances in fluorescence microscopy, fluorescent dyes, biotechnology, bioinformatics, and research on the human genome project in the late 90's accelerated the development of methods to synthesize and design NA probes for FISH. (springer.com)
Whether you select to visualize your RNA and DNA targets by fluorescence (FISH) or chromogenic (CISH) detection, you can be sure to find the best products and guidance for your workflow with our specialists. (ahdiagnostics.dk)
Time-resolved acquisition of the luminescent signal that occurs upon hybridization of the probe to the target enabled the avoidance of short-lived background fluorescence, markedly enhancing the sensitivity of detection, which was less than 1 pM. (njit.edu)
ECHO probes have a fluorescence-labeled nucleotide in which two molecules of thiazole orange or its derivatives are linked covalently. (ne.jp)
The clear change in fluorescence intensity depending on hybridization is useful for visible gene analysis. (ne.jp)
However, even this newly designed fluorescent probe exhibits a sequence-dependent change in the ratio of the fluorescence intensities for double- and single-stranded states. (ne.jp)
The probe self-dimer must be thermodynamically unstable because it contains many mismatched base pairs, but this plausible self-dimeric structure may inhibit fluorescence quenching because the formation of a 'primer dimer' in PCR experiments significantly decreases amplification efficiency. (ne.jp)
With πCode™ assay, any successful hybridization of probes and their specific targets would emit fluorescence, which is detected and analyzed by fully automated PlexBio™ 100 Fluorescent Analyzer. (affipcr.com)
Within a detection limit of 42 RNA copies per reaction, SHERLOCK was 100% specific and 100% sensitive with a fluorescence readout, and 100% specific and 97% sensitive with a lateral-flow readout. (nature.com)

Modified nucleic acids such as peptide nucleic acid (PNA) offer advantages compared to conventional DNA probes allowing for faster hybridization, better stability and minimal sample preparation for direct detection of pathogens. (psu.edu)

The detection of human papillomaviruses in histological preparations by using dot-blot hybridization. (adventube.ro)
Nucleic acid dot blot hybridization with fragmented digoxygenin labelled probes in human papillomavirus DNA screening. (adventube.ro)
3). Northern blot hybridization a. (ntu.edu.tw)
Northern blot hybridization d. (ntu.edu.tw)

Target amplification assays are based on the ability of complementary oligonucleotide primers to specifically anneal and allow enzymatic amplification of the target nucleic acid strands. (cdc.gov)
A single-stranded chemiluminescent DNA probe, which is complementary to a region of the target amplicon, is labeled with different acridinium ester molecule. (cdc.gov)
Such tests use a nucleic acid hybridization technique that is based on double-stranded complementary pairing of nucleic acids. (iso.org)
Basically, technicians synthesize a single-stranded nucleic acid sequence complementary to the DNA or RNA of a specific pathogen as a probe. (iso.org)
The digene HC2 High-Risk HPV DNA Test detects the presence of 13 high-risk HPV types (16/18/31/33/35/39/45/51/52/56/58/59/68, which are carcinogenic) using full genome probes complementary to HPV DNA, specific antibodies, signal amplification, and chemiluminescent detection. (qiagen.com)
It relies on the sequence-specific hybridization of probes to their complementary targets in individual cells, followed by direct or indirect detection of the labelled probe. (springer.com)
initially the capture NA strand is immobilised onto the electrode surface, this is then hybridised to the complementary target sequence, which is then followed by target hybridisation to a complementary probe strand which is labelled with a nanoparticle. (dcu.ie)
The ratio of quantum yields between after and before addition of the complementary nucleic acid strands showed a strong relationship with the number of possible Watson-Crick base pairs formed within two bases from D 514 in the 'self-dimer' of the probe. (ne.jp)
The target of ECHO probes is only the complementary single-stranded nucleic acid. (ne.jp)
Gillespie and Spiegelman (1) observed that single stranded DNA binds strongly to nitrocellulose membranes in a manner that minimises the two strands reassociating with each other, but allows the hybridisation to complementary RNA. (ddw-online.com)

This test, using Hybrid Capture 2 technology, is a nucleic acid hybridization microplate assay with signal amplification. (cdc.gov)
The digene HC2 High-Risk HPV DNA Test is an in vitro nucleic acid hybridization assay with signal amplification and chemiluminescence for the qualitative detection of 13 types of human papillomavirus (HPV) in cervical specimens. (qiagen.com)

Real-time polymerase chain reaction (PCR) technology has become a prominent tool in many molecular biology applications, including pathogen detection, gene expression analysis, and drug development, which propels the market expansion. (kingsresearch.com)

For 88 cervical samples (96 tests): Probe Diluent, High-Risk HPV Probe, Quality Controls, Calibrator, Capture Microplate, Reagents and Buffers (additional Wash buffer concentrate is available [cat. (qiagen.com)
Based on the knowledge and tools developed while identifying and characterizing new viruses and comparisons to previously-characterized viruses, new virus-specific and broad spectrum polyclonal and/or monoclonal antibody reagents, purification protocols, nucleic acid hybridization probes, PCR primers, isothermal amplification methods, and improved associated protocols will be developed. (usda.gov)
Miacom also holds additional patents in these fields including ID/AST and resistance detection, as well as reagents for respiratory sample preparation that are either currently being developed or already available on the market. (rapidmicromethods.com)
STREPTAMER(R) Best CELLer fluorescent MHC I Streptamer(R) reagents for detection and isolation of antigen-specific CD8+ T cells by flow cytometry select your. (biosave.com)
AMPIVIEW™ EGFR (AS) Dig RNA Probes are optimized for detection of EGFR RNA or RNA/DNA in FFPE tissues or cells with DIGX ® anti-digoxigenin linker, POLYVIEW® PLUS detection reagents combined with HIGHDEF ® chromogens and counterstains (linker and detection solutions not inlcuded). (enzolifesciences.com)

The individual DNA strands are called probes. (medscape.com)
The disc is pre-loaded with miRNA strands and each hybridization step is completed inside the disc by triggering the tabs of chambers to burst to release the contents. (dcu.ie)
DNA microarrays take advantage of the major feature of the DNA double helix, the sequence complementarity of the two-paired strands, by using DNA capture probes which are the complement of the expressed target sequence (mRNA, cRNA or cDNA made from the mRNA). (ddw-online.com)

In this web page, recent advances in nucleic acid detection using exciton-controlled hybridization-sensitive fluorescent oligonucleotide (ECHO) probe technologies are introduced to you. (ne.jp)

In bulk fluid assays, the dsPNAs had an overall higher fluorescent signal and better sensitivity and specificity than the PNA beacons for pathogen detection. (psu.edu)
Nucleic acid testing, which operates on this molecular level, has gradually become the mainstream of pathogen detection. (iso.org)
They would then label it using, for example, radioisotopes or enzymes, and subject it to hybridization with the nucleic acids of the pathogen to be tested. (iso.org)
If a probe can be complementarily paired with the nucleic acid of the pathogen to be tested, it is possible to observe the signals of the markers, so that the species of the pathogen to be tested can be confirmed. (iso.org)
and, (3) Characterize genomes of bacteria of major significance to ornamental and nursery crops to develop diagnostic tests for accurate pathogen detection. (usda.gov)
The long-term objective of this project is to develop effective means for the detection and identification of new and emerging plant viral and bacterial diseases of ornamentals, thus allowing growers to select pathogen-free or pathogen-indexed plants (tested for absence of specific pathogens) for propagation. (usda.gov)
This patent provides Miacom with extensive protection of their unique probe design and its application in providing pathogen detection by means of multiplex FISH assays. (rapidmicromethods.com)
A fast detection of the pathogen allows time to respond and then control the spread of the disease and avoid epidemics. (medicaldesignbriefs.com)
The regional market growth is proliferated by expanding applications in cancer research, pathogen detection, and environmental monitoring. (kingsresearch.com)

ISO/TS 5798 mainly focuses on the use of nucleic acid amplification methods for diagnosis and screening of SARS-CoV-2 and proposes requirements and recommendations for the design, development, validation, verification and implementation of testing methods. (iso.org)

It uses chemiluminescence for the qualitative detection of human papillomavirus (HPV) DNA in cervical specimens. (cdc.gov)

Nucleic acid hybridization is at the core of many amplification-free molecular diagnostics and detection probe configuration is key to diagnostic performance. (psu.edu)
3EO Health startup uses the Wyss Institute's user-friendly diagnostic nucleic acid detection technology together with additional components to build a digital platform that may empower patients with self-testing capabilities and access to results, information, and clinical partners. (harvard.edu)
BioGenex miRNA ISH Panel Probes are currently one of the best products for complex diagnostic assays. (biogenex.com)

This allows for the detection of each of the gene variants from the original sample. (wikipedia.org)
313919). Negative controls included Dap-B (dihydrodipicolinate reductase gene from Escherichia coli probe), an unrelated viral probe, and normal porcine brain region-matched sections. (cdc.gov)
Further applications of this method in prenatal diagnosis for the detection of aneuploidy of the most frequently involved autosomes, as well as for the quantification of gene copy number and mRNA expression, are discussed. (nih.gov)
A novel species-specific 14-bp PNA probe was designed for M. immunogenum based on its 16S rRNA gene sequence and was validated for specificity, by testing against a panel of other phylogenetically closely related rapidly growing mycobacteria and representative species of gram-positive, gram-negative, and acid fast organisms. (cdc.gov)
Because the locations of the probes are known, the intensity and pattern of the labeled mRNA can be used to measure the expression of the targeted gene. (medscape.com)
[ 6 ] Both technologies use hybridization of labeled nucleic acid transcripts to measure the gene expression. (medscape.com)
Tools that allow sensitive, quantitative, and real-time detection of specific transcripts, as well as the study of spatiotemporal gene regulation in living cells, are being developed. (ne.jp)

Here, we compared the sensitivity and specificity of double stranded PNA probes (dsPNA) and PNA molecular beacons targeting E. coli and P. aeruginosa for direct detection of bacterial pathogens. (psu.edu)
BioGenex unique SSNA miRNA ISH probes enable detection of single nucleotide mismatch thus allowing high sensitivity and specificity with a clean intense staining. (biogenex.com)
These novel luminescent labels should significantly enhance the sensitivity of all type of nucleic acid hybridization probes, and could dramatically improve the detection limit of other biopolymers and small compounds that are used in a variety of biological applications. (njit.edu)
This new technology also increases probe sensitivity and specificity to unprecedented levels. (rapidmicromethods.com)
Consequently, all samples were reevaluated on media containing cefotaxime (see Methods) to increase sensitivity for detection of ESC-resistant organisms. (cdc.gov)
Here, we report the clinical validation of the specific high-sensitivity enzymatic reporter unlocking (SHERLOCK) assay using the enzyme Cas13a from Leptotrichia wadei for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-the virus that causes coronavirus disease 2019 (COVID-19)-in 154 nasopharyngeal and throat swab samples collected at Siriraj Hospital, Thailand. (nature.com)
The development of new probe chemistries, such as hybridization probes and locked nucleic acid probes, has improved the sensitivity and specificity of real-time PCR assays. (kingsresearch.com)

The most dominant techniques are PCR viral detection and signal amplification DNA-based assays (Hybrid Capture II is the only FDA-approved technique at present). (medscape.com)

ISH was first demonstrated by Gall and Pardue ( 1969 ) using radioactive rRNA probes to visualize extrachromosomal rDNA. (springer.com)

DNA microarray), an appropriate receptor molecule, such as DNA oligonucleotide probes, antibodies, or other proteins, attach themselves to the differently labeled microspheres. (wikipedia.org)

That's partly because it can provide direct detection without prior isolation and culture of pathogens, making it convenient, fast and highly sensitive. (iso.org)
Isolation or detection of Neisseria gonorrhoeae from a disseminated site of infection (e.g., skin, synovial fluid, blood, or cerebrospinal fluid [CSF]) by culture or nucleic acid amplification test (NAAT). (cdc.gov)
Clinical manifestations of DGI without other known causes AND isolation or detection of Neisseria gonorrhoeae from a mucosal site of infection by culture or nucleic acid amplification test (NAAT). (cdc.gov)
A Laboratory Guide for Isolation and Characterization In Practical Nucleic Acid Hybridization in RNA Methodologies (4th Edition), Academic Press, New York. (ugm.ac.id)

2016, Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay. (ugm.ac.id)

The award of this patent by the U.S. Patent and Trademark Office further strengthens our position in in vitro diagnostics as it not only covers probe design but also their application. (rapidmicromethods.com)

BioGenex has developed proprietary Super Sensitive Nucleic Acid (SSNA) miRNA probes that enhance signals from intrinsically low populated miRNAs. (biogenex.com)
BioGenex miRNA probes are dual-end labeled on 3' and 5' with five FAM molecules that amplify the signal, giving intense stains. (biogenex.com)
The high-density fluorophore-labeled SSNA probes combined with the BioGenex Super Sensitive ™ Detection System aid in studying the lowly expressed miRNA populations and miRNA expression profiles to assess the physiological function of miRNA. (biogenex.com)
Over 240 ready-to-use (RTU) miRNA probes are now available, and more are being developed. (biogenex.com)
BioGenex SSNA miRNA probes combined with the automated processing using Xmatrx® greatly increases the reliability of the test results through the elimination of error-prone ISH procedure. (biogenex.com)
Using BioGenex ISH miRNA probes, the expression pattern of distinct miRNAs were successfully differentiated in wild type and BRCA mutated breast carcinomas. (biogenex.com)
Differential expression of miRNA was documented using the BioGenex Xmatrx® automated system and miRNA ISH BRCA breast panel probes. (biogenex.com)
When the hybridization is complete, the target miRNA which is specific to epilepsy, is detected based on the electrocatalytic reduction of hydrogen peroxide in phosphate buffer saline (PBS) at the PtNPs which are functionalised to the probe miRNA strand. (dcu.ie)

During the hybridization step, the sequence specific regions of the capture oligomers bind to specific regions of the target molecules. (cdc.gov)
The problem is exacerbated if non-sequence-specific probes, such as pH-sensitive dyes, are used for the detection 6 . (nature.com)

The GEN-PROBE TMA reaction replicates a specific region of the small ribosomal subunit from trichomonas vaginalis via DNA and RNA intermediates and generates RNA amplicon molecules. (cdc.gov)
Some techniques has applied to give high stringency condition avoid cross hybridization reaction but need more procedure to apply. (ugm.ac.id)
πCode™ MicroDiscs tagged with different capture agents are pooled, enabling specific detection of multiple analytes in one-well reaction. (affipcr.com)

31, 32 In this report we used a cDNA micro-array and two BAC clone micro-arrays on 10 previously identified cases containing 16 cryptic chromosome aberrations, to test their performance for the detection of small imbalances. (bmj.com)
Novel amino-reactive derivatives of lanthanide-based luminescent labels of enhanced brightness and metal retention were synthesized and used for the detection of cDNA oligonucleotides by molecular beacons. (njit.edu)

Probes with tethered fluorophore and quencher allow for solution-based assays and eliminate the need for washing steps thereby facilitating integration into microfluidic devices. (psu.edu)
Beyond the bulk fluid assay and droplet devices, use of dsPNA probes may be advantageous in a wide variety of assays that employ homogenous nucleic acid hybridization. (psu.edu)
In recent years, qPCR assays have been developed for various applications, including the detection of infectious diseases, genetic disorders, and cancer. (kingsresearch.com)

In ECHO probes, a hybridization-dependent fluorescent nucleotide regulated by the H-aggregation of thiazole orange organic dyes (D 514 ) is incorporated into specific sequence contexts and serves as fluorescent detection readout for target nucleic acids. (ne.jp)

The labeled DNA probe combines with amplicon to form stable RNA: DNA hybrids. (cdc.gov)

PCR round with Ni-PoAstV-3 specific inner primer pairs for the specific and sensitive detection of small quantities of viral RNA. (cdc.gov)
We applied specific or control probes and then incubated the slides at 40°C for 2 h. (cdc.gov)
Three chromosome-specific repetitive probes labeled with either amino acetyl fluorene (AAF), mercury, or biotin were hybridized simultaneously to metaphase chromosomes prepared from human blood lymphocytes or to interphase tumor nuclei. (nih.gov)
Detection and typing of HPV DNA in vaginal swabs (in conjunction with testing of NHANES sera for HPV antibody) will allow evaluation of trends in prevalence of type-specific HPV infection by age, sexual behavior, and race/ethnicity. (cdc.gov)
Specific and rapid detection and quantification of mycobacteria in contaminated metalworking fluid (MWF) are problematic due to complexity of the matrix and heavy background co-occurring microflora. (cdc.gov)
Currently, a range of probes can be synthesized to locate and quantify specific short RNAs, genes, entire chromosomes, and even cells (Evanko 2007 ). (springer.com)
Whipple's disease (WD) is a rare chronic systemic infection with a wide range of clinical symptoms, routinely diagnosed in biopsies from the small intestine and other tissues by periodic acid-Schiff (PAS) diastase staining and immunohistological analysis with specific antibodies. (frontiersin.org)
In 2010, Stoeckler and co-workers (4) reported that Cy3 double-labeling of FISH probes (at both ends) that were specific to ribsosomal RNA targets in microorganisms at least doubles FISH signal intensity without affecting specificity. (genelink.com)
Each πCode™ MicroDisc has an assigned distinct circular image pattern imprinted on its surface, and a specific capture agent, which can be either nucleic acid probe or antibody, conjugated to its surface. (affipcr.com)

Standard chromosome analysis by G-banding has a limited resolution, but molecular cytogenetic techniques, such as multi-subtelomeric FISH, microdeletion FISH, multicolour FISH and comparative genomic hybridisation (CGH), have played an important role for the diagnosis of MR during the past decade. (bmj.com)
The nucleic acid test is the 'gold standard' for pathogenic diagnosis, faster and more accurate than the other methods. (iso.org)
When using this method of diagnosis, specimen collection must occur prior to acetic acid or iodine use if colposcopy is to follow. (medscape.com)

Cy3 plays a particularly important role in real-time PCR applications, being used as the reporter moiety in TaqMan probes (1), Scorpion primers (2) and Molecular Beacons (3). (genelink.com)

The materials were then returned to CDC and SUNY for detection of residual viral infectivity. (cdc.gov)
Quantitative real-time PCR with reverse transcription (RT-qPCR), which measures the quantity of viral RNAs, remains the gold-standard technique for SARS-CoV-2 virus detection 1 . (nature.com)
RT-RPA and RT-LAMP are highly sensitive methods, but can suffer from nonspecific amplification under isothermal conditions, leading to false-positive results when used for viral RNA detections. (nature.com)

Among others, we have CE-IVD approved probes for the clinical laboratory, nick translation kits, and kits that makes it possible to study mRNA in solid tissue, and we are always ready to guide you to the best setup. (ahdiagnostics.dk)
You can get customized mRNA probes and our vendors have more than 6,500 mRNA off-the-shelf probes. (ahdiagnostics.dk)

But it also covers the steps of the overall process of pre-examination, examination and post-examination for the analysis of nucleic acid amplification method for the detection of SARS-CoV-2, and specifies the methods and comprehensive assessment indicators including accuracy, limit of detection, inclusivity, and specificity, etc. (iso.org)
J. (2010) Fast Molecular Beacon Hybridization in Organic Solvents with Improved Target Specificity, J. Phys. (ugm.ac.id)

3- 16 However, these probes only reveal chromosome rearrangements located in the subtelomeric region. (bmj.com)

Genetic analysis includes procedures for genotyping, SNP (single nucleotide polymorphism) detection, strain identification and various other procedures. (ddw-online.com)

AMPIVIEW™ NSP Dig RNA probes (no signal) detected in prostate hyperplasia tissue specimen with DIGX® rabbit anti-digoxigenin linker (ENZ-ABS303), POLYVIEW® PLUS AP (anti-rabbit) (ENZ-ACC110), combined with HIGHDEF® Red Chromogen/Substrate and Hematoxylin counterstain (ENZ-ACC106). (enzolifesciences.com)

This is the optimized probe length as it minimizes the melting temperature variation among different probes during probe-target hybridization. (wikipedia.org)
Early studies of DNA melting and reformation were carried out in aqueous solutions and yielded important information about the dependence of melting temperature (Tm) on the G+C composition and salt concentration, as well as information on the dependence of the rate of reassociation on the sequence complexity of the nucleic acid. (ddw-online.com)

There are currently three methods for detecting SARS-CoV-2: nucleic acid tests, antigen tests and antibody tests. (iso.org)
NALF based on nucleic acid hybridizationis more economical then immunochromatography assay which use antibody-antigen recognition. (ugm.ac.id)

We have more than 250 CE-IVD approved probes for solid tumors, hematology, and pathogens. (ahdiagnostics.dk)
Improved detection and differentiation methods for these pathogens will enable state and federal regulatory officials to make timely and appropriate recommendations in safeguarding the movement of horticultural and agricultural products into the United States. (usda.gov)

We show that FISH not only detect active WD but also be helpful as an indicator for the efficiency of antibiotic treatment and for detection of recurrence of disease when the signal of PAS diastase and immunohistochemistry lags behind the recurrence of disease, especially if the clinical course of the patient and antimicrobial treatment is considered. (frontiersin.org)

The direct DNA hybridization approach is the simplest suspension array assay whereby 15 to 20 bp DNA oligonucleotides attached to microspheres are amplified using PCR. (wikipedia.org)

We report the detection of HPV 52 in a sample taken from a human papillomavirus infection lesion patient with squamous cell carcinoma of the conjunctiva of the left eye. (bethlen-foundation.ro)

B.D. (1985) Quantitative filter hybridization. (ugm.ac.id)
Nucleic acid detection by isothermal amplification and the collateral cleavage of reporter molecules by CRISPR-associated enzymes is a promising alternative to quantitative PCR. (nature.com)

As a positive control for nucleic acid integrity in the tissue sections, we used a probe that hybridizes to a conserved region of the highly expressed PPIB of M. musculus (Cat. (cdc.gov)
The initial and important step of FISH, CISH and ISH is to prepare your tissue for hybridization, before adding probe. (ahdiagnostics.dk)
Positive hybridization signals were identified in interstitial mononuclear cells and in bronchi-associated lymphoid tissue from 3 to 11 PID. (nebraska.edu)

This temperature reduction allows hybridization to occur between the deoxyadenosine region on the capture oligomer and the poly-deoxythymidine molecules that are covalently attached to the magnetic particles. (cdc.gov)

We further demonstrated spatially multiplexed μFISH, enabling the use of spectrally equivalent probes for detailed and real-time analysis of a cell monolayer, which paves the way towards rapid and automated multiplexed FISH on standard cytological supports. (springer.com)
μFISH enables a rapid nucleic acid analysis, and we demonstrate its usefulness for spatially multiplexed FISH, Fig. 1 . (springer.com)

Nucleic acid amplification tests (NAATs) are the recommended test method. (cdc.gov)
The common nucleic acid detection method includes direct DNA hybridization. (wikipedia.org)
This method is compatible with the standard workflow of conventional FISH, allows re-budgeting of the sample for various tests, and results in a ~ 15-fold reduction in probe consumption. (springer.com)
Here, we describe a microfluidic-based method which enables the detection of microRNAs or miRNAs in single intact cells by flow-FISH using locked nucleic acid (LNA)-containing probes. (nih.gov)
For demonstration of our method, this chapter details the detection of miR155 and CD69 protein in PMA and ionomycin-stimulated Jurkat cells. (nih.gov)
Electrochemical Impedance Spectroscopy (EIS) was also performed on the modified electrode as another method of NA detection. (dcu.ie)
Lateral flow assay (LFD) based nucleic acid lateral flow (NALF) method has been developed recently. (ugm.ac.id)

Test fragments and probes on the microsphere beads are allowed to hybridize to each other. (wikipedia.org)
Detection of polynucleotide fragments using hybridization with probes labelled with digoxigenin. (adventube.ro)

Microspheres can be sorted based using both their unique optical properties and level of hybridization to the target sequence. (wikipedia.org)

Probe-target hybridization is usually detected by optically labeled targets, which determines the relative abundance of each target in the sample. (wikipedia.org)
After amplifying one DNA oligoprobe of interest, it can be used to create 100 different probes on 100 different sets of microspheres, each with the capability of capturing 100 potential targets (if using a 100-plex array). (wikipedia.org)
Multiplexed FISH is often used to locate multiple targets in a cell nucleus simultaneously using probes of different colors, in order to study sparse cytological substrates (Lichter 1997 ). (springer.com)
Optimal probe length varies for targets with high sequence variation: implications for probe library design for resequencing highly variable genes. (ugm.ac.id)

The probe containing a 120-bp repetitive DNA sequence from rye, hybridized to the entire length of all rye chromosomes, but only to a few sites in 14 wheat chromosomes. (illinois.edu)
The overall distribution of this DNA probe in the rye chromosomes has not been detected previously with the use of radioactively labeled probes. (illinois.edu)
As a result of the formation of a brown precipitate over sites of hybridization in this technique, the rye chromosomes were entirely brown in color, whereas the wheat chromosomes appeared blue. (illinois.edu)

The extended culture approach enables the detection of slower-growing organisms such as anaerobic bacteria like lactobacillus and enterococcus. (uk.com)
In: Nucleic Acid Hybridization, a Practical Approach. (ugm.ac.id)

Anatomy1

Organisms4

Chemicals and Drugs28

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Phenomena and Processes17

Disciplines and Occupations1

Information Science4

Health Care6

Situ23

Sequences12

Fluorescence10

Peptide Nucl1

Blot hybridization4

Complementary10

Assay with signal amplification2

Polymerase Chain Re1

Reagents5

Strands3

Fluorescent oligonucleotide1

Pathogen9

Amplification methods1

Chemiluminescence for the qualitative detection1

Diagnostic3

Gene7

Sensitivity7

Signal amplification1

RRNA1

Oligonucleotide probes1

Isolation4

20161

Vitro1

MiRNA8

Sequence specific2

Reaction3

CDNA2

Assays3

Readout1

Amplicon1

Specific9

Diagnosis3

Primers1

Viral3

MRNA2

Specificity2

Chromosome1

Single nucleotide1

Specimen1

Melting temperature2

Antibody2

Pathogens2

Antibiotic1

Oligonucleotides1

Infection1

Quantitative2

Tissue3

Molecules1

Analysis2

Method7

Fragments2

Target sequence1

Targets4

Chromosomes3

Approach2