• Herein, we present a new strategy for bovine serum albumin (BSA) detection using a novel colour-switch fluorescent probe CPV-ESF ((E)-2-(4-((Z)-1-cyano-2-(4-(diethylamino)phenyl)vinyl)phenyl)ethene-1-sulfonyl fluoride). (edu.au)
  • The assay could detect down to 0.05 μM of HSA, and also exhibited good selectivity for HSA over bovine serum albumin and other biomolecules such as ATP, β-amyloid, peroxidase, thrombin, lysozyme, trypsin, hemoglobin, and the various nucleic acids. (edu.hk)
  • Crude antigenic preparations from Setaria equina were used in ELISA and Western blotting to examine cross-reaction with human sera from areas endemic for bancroftian filariasis. (who.int)
  • Des préparations d'antigènes bruts de Setaria equina ont été utilisées dans le cadre des méthodes ELISA et transfert Western afin d'étudier la réaction croisée avec des sérums humains en provenance de zones endémiques pour la filariose de Bancroft. (who.int)
  • Methods: Enzyme-linked immunosorbentassay (ELISA) was carried out in serum samples collected from 40 patients to detect anti-toxoplasma IgG and IgM antibodies as a part of work up in suspected cases. (eurekaselect.com)
  • A set of 13 serum samples (3 from SCLC and 10 from NSCLC patients) was screened by enzyme-linked immunosorbent assay (ELISA), using a commercially available kit. (astm.org)
  • HA516 trade name] is indicated for the treatment of chronic hepatitis B in adults with: · compensated liver disease and evidence of immune active disease, i.e. active viral replication, persistently elevated serum alanine aminotransferase (ALT) levels and histological evidence of active inflammation and/or fibrosis. (who.int)
  • Due to the low sensitivity of the current diagnosis systems, a new sensitive detection method is needed for its diagnosis. (figshare.com)
  • This method greatly increases the sensitivity and specificity of the biomarker detection. (figshare.com)
  • The sensing mechanism, sensitivity and selectivity of CPV-ESF for BSA detection as well as its application for cell imaging have been investigated. (edu.au)
  • Conclusions: A doubling factor is effective for maintaining the sensitivity and specificity of PSA for prostate cancer detection in men on dutasteride. (wustl.edu)
  • We also compared different blood sample types and suggest a higher sensitivity by serum/plasma over whole blood. (lu.se)
  • Comparison of Two Diagnostic Assays for the Detection of Serum Neutra" by Justin Brown, Korakrit Poonsuk et al. (unl.edu)
  • The majority of antibody detection tests for parasitic diseases in the United States are now performed in several commercial laboratories: Focus Technologies, Mayo Medical Laboratories, Parasitic Disease Consultants, Quest Diagnostics, and Specialty Laboratories. (cdc.gov)
  • Only a few commercial kits are available in the U.S. for antibody detection of parasitic diseases. (cdc.gov)
  • Histoplasma antigen and anti- Histoplasma antibody detection are used to support the diagnosis of histoplasmosis . (bvsalud.org)
  • Our primary hypothesis was that anti- Histoplasma immunoglobulin G ( IgG ) antibody detection using enzyme immunoassay (EIA) will be more sensitive as compared to immunodiffusion ( ID ). (bvsalud.org)
  • CONCLUSION AND CLINICAL IMPORTANCE Antibody detection by EIA can be used to support the diagnosis of histoplasmosis in cats and dogs . (bvsalud.org)
  • Bhattacharyya S, Khurana S, Dubey ML. Anti-Toxoplasma gondii antibody detection in serum and urine samples by enzyme-linked immunosorbent assay in HIV-infected patients. (eurekaselect.com)
  • In PCR-confirmed COVID-19 patients (n = 45), the total antibody detection rate is 92% in hospitalized patients and 79% in non-hospitalized patients. (lu.se)
  • Our study provides a comprehensive validation of the rapid COVID-19 IgM/IgG serology test, and mapped antibody detection patterns in association with disease progress and hospitalization. (lu.se)
  • Serum proteins are classified as albumin or globulins. (medlineplus.gov)
  • Albumin is the most abundant protein in the serum. (medlineplus.gov)
  • A novel cyclometalated iridium(III) complex has been developed as a luminescent probe for human serum albumin (HSA) detection in aqueous solution. (edu.hk)
  • In this system, the initial luminescence of the iridium(III) complex in the absence of human serum albumin is weak. (edu.hk)
  • The next step is to identify the specific biomarkers that make early detection possible. (reading.ac.uk)
  • Moreover, comparing serum Taurine level and specific biomarkers before and after surgical treatment. (scitcentral.com)
  • The detection limit of our nanobiotechnological method is approximately 1.1 pg/mL, about 2 orders of magnitude lower than that of the traditional antigen-based method, opening up a new avenue to virus-based disease diagnosis. (figshare.com)
  • Based on the high dissemination potential of ZIVK and its similar antigen composition to other arboviruses, new approaches for selective virus detection are urgently needed. (butantan.gov.br)
  • HBV) DNA loss with d detection of antibodies against HBeAG) or - until Hepatitis B surface antigen (HBsAG) seroconversion or - until loss of efficacy (see section 4.4). (who.int)
  • The method is sensitive and rapid enough to screen serum specimens from subjects suspected to be exposed to a number of important toxic elements, or to evaluate environmental or other nonoccupational exposure to these same elements. (cdc.gov)
  • Serum specimens are processed, stored, and shipped to the Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA for analysis. (cdc.gov)
  • Most drug assays are based on total concentrations of bound and unbound proteins in the serum, although only the unbound concentration causes beneficial and adverse events. (elsevierpure.com)
  • Here, we aimed to determine the predictive value of serum glycans to distinguish non-small cell lung cancer (NSCLC) cases from controls in prediagnostic samples using a previously validated predictive protein marker pro-SFTPB, as anchor. (nih.gov)
  • Our previous studies have identified a serum-based 4-microRNA (4-miRNA) signature that may help distinguish patients with lung cancer (LC) from non-cancer controls (NCs). (vumc.org)
  • Comparison of the extraction and determination of serum exosome and miRNA in serum and the detection. (irdrjournal.com)
  • Comparison of the extraction and determination of serum exosome and miRNA in serum and the detection of miR-27a-3p in serum exosome of ALS patients. (irdrjournal.com)
  • The goal of this study is to investigate if it is possible to develop a serological test that, in chicken serum, can differentiate between antibodies against different Chlamydia species, particularly C. psittaci and C. gallinacea , using peptides as antigens. (biomedcentral.com)
  • Here, we have used a recombinant scFv antibody microarray in an attempt to classify sera derived from pancreatic adenocarcinoma patients vs. healthy subjects. (lu.se)
  • Based on analysis of non-fractionated, directly labeled, whole human serum proteomes we have identified a protein signature based on 19 non-redundant analytes, that discriminates between cancer patients and healthy subjects. (lu.se)
  • Previous studies have suggested occurrence of altered serum glycan profiles in patients with lung cancer. (nih.gov)
  • Cross-reaction was found between S. equina antigens and antibodies in the sera of Wuchereria bancrofti-infected patients, with the highest levels observed between sera of chronic infected patients and Setaria spp. (who.int)
  • Therefore, we investigated the effects of patients' sera on endothelia cells (ECs) in vitro. (mdpi.com)
  • The epidemiology of aminoglycoside-associated acute kidney injury (AG-AKI) has not been well described in pediatric patients with cystic fibrosis (CF). We aimed to assess the impact of daily serum creatinine (SCr) measurement on detection of AG-AKI at our institution. (blogspot.com)
  • Daily SCr measurement promoted earlier and increased detection of AG-AKI in patients with CF at our institution. (blogspot.com)
  • Toward this end, here we exploited the capability of genetically displaying two functional peptides, one responsible for recognizing the biomarker for the infection (antisecreted aspartyl proteinase 2 IgG antibody) in the sera of cancer patients and another for binding magnetic nanoparticles (MNPs), on a single filamentous fd phage, a human-safe bacteria-specific virus. (figshare.com)
  • We have searched for the presence of genetic alterations in serum DNA obtained from 44 colorectal cancer patients. (johnshopkins.edu)
  • Comparison of the molecular analysis with clinical diagnosis of these patients revealed that none of the seven Dukes' stage B patients with a K-ras mutation in their tumors demonstrated a mutation in the serum. (johnshopkins.edu)
  • In contrast, five of seven stage B patients with a p53 mutation in the tumor demonstrated a mutation in the paired serum (P = 0.01, Fisher's exact test). (johnshopkins.edu)
  • Taken together, either a K-ras or p53 mutation was detected in the serum in 40% of the 25 patients (95% confidence interval, 21- 61%), whose primary tumors contained a mutation and in 23% of the 44 patients (95% confidence interval, 12-38%) with colorectal cancer. (johnshopkins.edu)
  • The frequent detection of p53 mutation in the serum of patients with early stage tumors suggests a possible use of this approach for clinical prognosis and cancer monitoring of colorectal cancer patients. (johnshopkins.edu)
  • Therefore, we suppose that the expression of miR-27a-3p in the serum exosomes of ALS patients also changes. (irdrjournal.com)
  • Then through comparison of the expression of miR-27a-3p in the serum exosomes of 10 ALS patients and healthy subjects, we found that in the ALS patients miR-27a-3p was down-regulated, and may be involved in the development of ALS, and therefore has potential as a reference for the diagnosis of ALS in the clinic. (irdrjournal.com)
  • The level of cyclo(His-Pro) in sera of individuals with hypothyroidism, hyperthyroidism, or alcoholic cirrhosis was in the normal range, while patients with renal failure had approximately 3-fold elevated levels of the peptide. (elsevierpure.com)
  • This finding prompted us to look for semenogelin expression in the serum of lung cancer patients. (astm.org)
  • In this study, we investigated the role of serum HE4 in comparison to CA125 in the detection of recurrent disease in patients during follow-up.615 ovarian cancer patients treated at Shandong Provincial Tumor Hospital between 2008 and 2014 were included in the retrospective study. (journalcra.com)
  • Investigate the probability of using serum Taurine level as a pre- early biomarker for colorectal carcinoma, especially in the precancerous condition in Egyptian patients. (scitcentral.com)
  • Toxin detection may help identify patients who would benefit from antibiotic treatment and could lower the unnecessary use of antibiotics. (mlo-online.com)
  • Test specificity was determined to be 97% on 69 sera/plasma samples collected between 2016-2018. (lu.se)
  • A novel strategy for quantitative profiling of serum or plasma proteomes is described. (nist.gov)
  • Circulating neutralizing antibodies levels in sow sera may help determine if a detectable immune response could confer protection to piglets. (unl.edu)
  • Iga Serum Laboratories manufactures the detectable serum iga reagents distributed by Genprice. (zebov-detection.com)
  • The Detectable Serum Iga reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (zebov-detection.com)
  • Serum Glycans as Risk Markers for Non-Small Cell Lung Cancer. (nih.gov)
  • Validation of a serum 4-microRNA signature for the detection of lung cancer. (vumc.org)
  • We next used an oligonucleotide- mediated mismatch ligation assay to detect tumor specific gene mutations in the serum. (johnshopkins.edu)
  • Furthermore, the application of the assay for the detection of HSA in diluted urine samples and for monitoring the conformational changes of HSA induced by exogenous protein unfolding agents was also demonstrated. (edu.hk)
  • Serum HE4 and CA125 were analyzed during follow-up using the ARCHITECT assay. (journalcra.com)
  • A nested case control study was undertaken on stored serial serum samples from women who developed ovarian cancer and healthy controls (matched for serum processing and storage conditions as well as attributes such as age) in a pilot randomized controlled trial of ovarian cancer screening. (reading.ac.uk)
  • This paper demonstrates that mass spectra from the low molecular weight serum proteome carry information useful for early detection of ovarian cancer. (reading.ac.uk)
  • These data suggest HE4 is a more sensitive and specific predictor of recurrent disease and should therefore be incorporated with CA125 in detection of recurrent ovarian cancer. (journalcra.com)
  • Serum from 'non-immune' Europeans did not agglutinate infected erythrocytes, however, in the immunofluorescence test with anti-Ig and anti-F(ab')2 secondary reagents we could detect the binding of IgG antibody from 'non-immune' European serum to a small proportion of infected cells. (ox.ac.uk)
  • Among the 16 cases with a K-ras gene mutation in the tumor, the same mutation was detected in three paired serum samples. (johnshopkins.edu)
  • In the 10 cases with a p53 mutation in the tumor, the identical mutation was detected in seven corresponding serum samples. (johnshopkins.edu)
  • Could it better to use Serum Taurine Level as a Biomarker for Colorectal Carcinoma Early Detection instead of the Specific Tumor Marker? (scitcentral.com)
  • it is more valuable and more accurate early biomarker for early detection of any malignant change which may led to CRC by other means it is the most sensitive and more specific tumor marker for CRC. (scitcentral.com)
  • HER-2/neu in serum is a specific tumor marker and only slight elevations may be found in the absence of malignancy, mainly in association with liver diseases. (degruyter.com)
  • Likewise, in most of the studies published, a relationship has been found between serum HER-2/neu levels (either pretreatment or at follow-up) with tumor response. (degruyter.com)
  • Herein, we report an aptamer-based field effect transistor (aptaFET) biosensor, developed by using an extended gate field effect transistor with inter-digitated gold microelectrodes (IDμE) for the detection of the malaria biomarker Plasmodium falciparum glutamate dehydrogenase (PfGDH) in serum samples. (bath.ac.uk)
  • This biosensor exhibited a sensitive response in broad dynamic range of 100 fM - 10 nM with limits of detection of 16.7 pM and 48.6 pM in spiked buffer and serum samples, respectively. (bath.ac.uk)
  • We designed a protein biosensor that uses thermodynamic coupling for sensitive and rapid detection of neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in serum. (nature.com)
  • The biosensor is a switchable, caged luciferase-receptor-binding domain (RBD) construct that detects serum-antibody interference with the binding of virus RBD to angiotensin-converting enzyme 2 (ACE-2) as a proxy for neutralization. (nature.com)
  • However, detection of specific antibodies in a person native to an area where the parasite is endemic may reflect only a past infection unrelated to current clinical status. (cdc.gov)
  • We offer a comprehensive range of 4000+ clinical laboratory tests and profiles, which are used for prediction, early detection, diagnostic screening, confirmation and/or monitoring of the disease. (metropolisindia.com)
  • Purpose: We determined whether the decrease in serum PSA seen with 5α-reductase inhibitors affects the clinical usefulness of PSA for prostate cancer screening using data from 2 dutasteride benign prostatic hyperplasia studies. (wustl.edu)
  • This work reports the development of an electrochemical immunoassay for detection of anti-ZIKV antibodies, using magnetic beads functionalized with recombinant protein derived from the non-structural protein 1 (ΔNS1-ZIKV) and anti-IgG antibodies labeled with horseradish peroxidase (HRP) enzyme. (butantan.gov.br)
  • The magneto-immunoassay uses disposable microfluidic devices for detection of anti-ZIKV in serum samples. (butantan.gov.br)
  • The external domain of this protein is released by the cell and can be studied in serum by immunoassay. (degruyter.com)
  • Serum beta-D glucan assays are nonspecific but can support the diagnosis. (msdmanuals.com)
  • We have previously developed the hiVNT system which detects SARS-CoV-2 neutralizing antibodies in sera in less than three hours. (medrxiv.org)
  • Serum glycan profiles were determined by mass spectrometry. (nih.gov)
  • Additionally, in the ultrafiltered human serum, the obtained values of unbound doxorubicin concentration showed good agreement with those quantified by conventional liquid chromatography-mass spectrometry. (elsevierpure.com)
  • Is the Subject Area "Mutation detection" applicable to this article? (plos.org)
  • In the presence of active S. equina infection, antigens from the adult and microfilaraemic stages showed the highest degree of cross-reaction with human sera. (who.int)
  • Analysis of human sera drawn at 3-h intervals over a 24-h period suggested a circadian rhythm of cyclo(His-Pro) levels. (elsevierpure.com)
  • Our goal in this study was to develop and validate a methodology that uses machine learning (ML) to accurately predict colorectal cancer status based on oncRNA profiles detected in patient sera. (exai.bio)
  • Parasite-infected-cell-agglutination and indirect immunofluorescence assays for detection of human serum antibodies bound to antigens on Plasmodium falciparum-infected erythrocytes. (ox.ac.uk)
  • Two methods are described for detecting the binding of serum antibodies from adults in an endemic malarious area (The Gambia) to surface antigens on Plasmodium falciparum-infected erythrocytes. (ox.ac.uk)
  • Additionally, analytical methods are included that modify previously used methods to obtain lower detection limits, and/or to improve accuracy and precision. (cdc.gov)
  • As the use of serological methods increases the window of detection, the goal of this study was to investigate if it is possible to develop a differentiating serological test for antibodies against Chlamydia species in chicken sera. (biomedcentral.com)
  • In this study, we used healthy human serum as a sample to find out the conditions and methods for extraction and detection. (irdrjournal.com)
  • Detection of antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which causes COVID-19 by serological methods is important to diagnose a current or resolved infection. (lu.se)
  • There are numerous research laboratories, both public and private, that are developing different early-detection tests for cancer. (medscape.com)
  • This project attempts to reveal whether within individual serum testosterone concentrations might be sufficiently stable biologically in the normal individual to provide a useful marker to add to the athlete biological passport to evidence anabolic steroid administration. (wada-ama.org)
  • Sera from normal subjects from non-endemic areas were included as negative controls. (who.int)
  • There has been a continuous strive to develop portable, stable, sensitive and low cost detection system for malaria to meet the demand of effective screening actions in developing countries where the disease is most endemic. (bath.ac.uk)
  • Increases in serum PSA in men receiving dutasteride should be considered suspicious and serial PSA measurements should be used to evaluate changes from nadir. (wustl.edu)
  • Nevertheless, results of the suspension array with field sera are supported by published data on the occurrence of C. gallinacea in Dutch layers, thereby demonstrating the proof of concept of multiplex serology for Chlamydial species in poultry. (biomedcentral.com)
  • Here, we have developed a rapid electrochemical method to determine the unbound concentration in ultrafiltered human serum using boron-doped diamond (BDD) electrodes. (elsevierpure.com)
  • When the anticancer drug doxorubicin was used as the test drug, the catalytic doxorubicin-mediated reduction of dissolved oxygen provided a sensitive electrochemical signal, with a detection limit of 0.14 nM. (elsevierpure.com)
  • In contrast to the results with freshly collected isolates, antibodies from sera of Gambian adults did not bind to the surface of infected cells from five different culture-adapted isolates of P. falciparum. (ox.ac.uk)
  • Blinded prediagnostic serum samples were obtained from the Carotene and Retinol Efficacy Trial (CARET), and included a discovery set of 100 NSCLC cases and 199 healthy controls. (nih.gov)
  • Four glycan variables exhibited significant incremental value when combined with pro-SFTPB compared with pro-SFTPB alone with AUCs of 0.73, 0.72, 0.72, and 0.72 in the test set, indicating that serum glycan signatures have relevance to risk assessment for NSCLC. (nih.gov)
  • For the majority of these peptides, species-specific seroreactivity in mammalian sera has been reported in literature. (biomedcentral.com)
  • In field sera, signals were difficult to interpret as insufficient sera from experimentally infected chickens were available for evaluating the seroreactivity of all peptides. (biomedcentral.com)
  • Since C. gallinacea and C. psittaci can infect chickens and are likely to differ in epidemiology and zoonotic potential, it is important to have diagnostic tools available that allow detection of, and distinction between, these two species, both from a public health perspective as from an economic point of view. (biomedcentral.com)
  • HA516 trade name] is indicated for the treatment of chronic hepatitis B in adolescents 12 to 18 years of age and weighing at least 35 kg with: · compensated liver disease and evidence of immune active disease, i.e. active viral replication, persistently elevated serum ALT levels and histological evidence of active inflammation and/or fibrosis. (who.int)
  • Serum ALT and HBV DNA levels should be followed regularly after treatment discontinuation to detect any late virological relapse. (who.int)
  • What Is the Future for Multicancer Early Detection Tests? (medscape.com)
  • PARIS - Multicancer early detection blood (MCED) tests are the focus of intensive development. (medscape.com)
  • Early detection and effective treatment of cervical precancerous lesions are the key to preventing cervical cancer. (hindawi.com)
  • 1995). The maximum detection limits (MCLs) for Aroclors vary in the range of 0.054-0.90 µ g/L in water and 57-70 µg/kg in soils (EPA 1995c). (cdc.gov)
  • Molecular diagnostics and expanding access to these tools may remedy chronic under-detection and under-reporting in affected areas. (medscape.com)
  • Serum sample were collected from each gilt prior to and following challenge with porcine epidemic diarrhea virus. (unl.edu)
  • The average detection time for each serum sample is only about 6 h, much shorter than the clinically used gold standard method, which takes about 1 week. (figshare.com)
  • This lab test measures the types of protein in the fluid (serum) part of a blood sample. (medlineplus.gov)
  • Detection of antibodies can be very useful as an indicator that an individual has been infected with a specific parasite. (cdc.gov)
  • The detection of specific IgM and IgA antibodies may be of value in determining the approximate time of initial infection with Toxoplasma gondii , but is not recommended for any other parasitic disease. (cdc.gov)
  • A specific antiserum directed against cyclo(His-Pro) was used to detect immunoreactive material in human serum. (elsevierpure.com)
  • Semenogelins, the major protein constituents of semen coagulum, are the most effective markers for semen detection. (astm.org)
  • A procedure for estimation of the content of free cyclo(His-Pro) in human serum was developed. (elsevierpure.com)
  • Objective: The Working Group on Uric Acid and Cardiovascular Risk of the Italian Society of Hypertension conceived and designed an ad-hoc study aimed at searching for prognostic cut-off values of serum uric acid (SUA) in predicting fatal myocardial infaction (MI) in women and men. (unina.it)
  • The available differentiating tests for Chlamydia are based on detection of genetic material and only give information about the actual infection status, but reveal nothing of past infections. (biomedcentral.com)