Detection of nucleic. Medical search. Frequent questions

Detecting gamma-irradiated SARS-CoV-2 virions in an extraction buffer, the current amplified HCR lateral flow assay achieves a limit of detection of 200 copies/µL using nucleic acid probes to target the SARS-CoV-2 RNA genome. (caltech.edu)
By swapping out nucleic acid probes to target different pathogens, amplified HCR lateral flow assays offer a platform for adaptable and sensitive at-home testing for emergent diseases. (caltech.edu)
Modified nucleic acids such as peptide nucleic acid (PNA) offer advantages compared to conventional DNA probes allowing for faster hybridization, better stability and minimal sample preparation for direct detection of pathogens. (psu.edu)
Here, we compared the sensitivity and specificity of double stranded PNA probes (dsPNA) and PNA molecular beacons targeting E. coli and P. aeruginosa for direct detection of bacterial pathogens. (psu.edu)
We further designed and tested an expanded panel of dsPNA probes for detection of a wide variety of pathogenic bacteria including probes for universal detection of eubacteria, Enterobacteriaceae family, and P. mirablis. (psu.edu)
Beyond the bulk fluid assay and droplet devices, use of dsPNA probes may be advantageous in a wide variety of assays that employ homogenous nucleic acid hybridization. (psu.edu)
The problem is exacerbated if non-sequence-specific probes, such as pH-sensitive dyes, are used for the detection 6 . (nature.com)
In the process of PCR extension, the exonuclease activity of Taq enzyme will cut off the 5'end fluorescent groups from the probes, freeing them in the reaction system, thus breaking away from the shielding of the 3'end fluorescence quenching groups, which can receive light stimulation and emit fluorescence that can be detected by the instrument, so as to achieve the automatic detection of HSV II nucleic acid in closed reaction system. (mmtegypt.com)
The primers and probes are designed according to the internal control template, the internal control adopts VIC channel, so as to realize the detection process monitoring in closed reaction system, which can effectively monitor the occurrence of false negative results. (mmtegypt.com)
These novel luminescent labels should significantly enhance the sensitivity of all type of nucleic acid hybridization probes, and could dramatically improve the detection limit of other biopolymers and small compounds that are used in a variety of biological applications. (njit.edu)
ABSTRACT: Nucleic acid sequence based amplification (NASBA) is an isothermal nucleic acid amplification procedure based on target-specific primers and probes, and the co-ordinated activity of 3 enzymes: AMV reverse transcriptase, RNase H, and T7 RNA polymerase. (int-res.com)
Several methods have been devised recently for very sensitive detection of variola virus DNA and to distinguish this DNA from that of other orthopoxviruses, the most promising being analysis by polymerase chain reaction (PCR) of restriction fragment length polymorphisms, multiplex PCR and real-time PCR with fluorigenic probes. (who.int)

The detection and quantitation of specific nucleic acid (NA) sequences continue to grow in importance. (dcu.ie)
Primers for RT-LAMP or RT-RPA can be designed for specific viral RNA sequences, and the amplification methods are used before the Cas enzyme detection step, to multiply detection signals from the specific nucleic acid sequences. (nature.com)

But that was still not enough for detection, so we moved the whole assay from a fluorescence plate reader, which is quite large and not available in low-resource settings, to an electrochemical sensor, which has higher sensitivity and can be used for point-of-care diagnostics," she said. (technologynetworks.com)
The detection limit achieves 0.26 attomole, and the multiplexed assay takes only 40 min. (ufluidix.com)
Here, we report the clinical validation of the specific high-sensitivity enzymatic reporter unlocking (SHERLOCK) assay using the enzyme Cas13a from Leptotrichia wadei for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-the virus that causes coronavirus disease 2019 (COVID-19)-in 154 nasopharyngeal and throat swab samples collected at Siriraj Hospital, Thailand. (nature.com)
The assay, which we show is amenable to multiplexed detection in a single lateral-flow strip incorporating an internal control for ribonuclease contamination, should facilitate SARS-CoV-2 detection in settings with limited resources. (nature.com)
In the third study, phoB was further validated as a diagnostic target for the species specific detection of H. influenzae by targeting the phoB mRNA transcript in a duplex real-time NASBA diagnostic assay. (nuigalway.ie)
The purpose of this study was to develop a real time polymerase chain reaction ( PCR ) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories .Sanger sequencing and a newly developed locked nucleic- acid , real-time PCR assay were used to detect the JAK2V617F mutation . (bvsalud.org)
FIND) has partnered with Cepheid, Inc. (Sunnyvale, CA) and the University of Medicine and Dentistry of New Jersey (UMDNJ, Newark, NY) to develop a TB-specific automated, cartridge-based nucleic amplification assay (Xpert MTB/RIF) based on the GeneXpert multi-disease platform, currently unique in its simplification of molecular testing with fully integrated and automated sample preparation, amplification and detection required for real-time polymerase chain reaction. (bvsalud.org)

Nucleic acid detection by isothermal amplification and the collateral cleavage of reporter molecules by CRISPR-associated enzymes is a promising alternative to quantitative PCR. (nature.com)
RT-RPA and RT-LAMP are highly sensitive methods, but can suffer from nonspecific amplification under isothermal conditions, leading to false-positive results when used for viral RNA detections. (nature.com)
Quantitative real- time reverse transcriptase - polymerase chain reaction (RT-qPCR) kits have been reliably used for detection of SARS-CoV-2 RNA since the beginning of the COVID-19 outbreak, whereas isothermal nucleic acid amplification-based point-of-care automated kits have also been considered as a simpler and rapid alternative. (bvsalud.org)

Fast, inexpensive, and multiplexed detection of multiple nucleic acids is of great importance to human health, yet it still represents a significant challenge. (ufluidix.com)

An integrated nucleic acid detection method and detection reagent tube (ZL201810669579.9) patent certificate_USTAR BIOTECHNOLOGIES (HANGZHOU) LTD. (bioustar.com)

Here we describe how to detect nucleic acid-protein interactions in plant leaves by using a fluorescence resonance energy transfer (FRET) approach coupled to fluorescence lifetime imaging microscopy (FLIM). (nature.com)
This phenomenon has been efficiently harnessed by SHERLOCK (Specific High-sensitivity Enzymatic Reporter unLOCKing) and DETECTR (DNA Endonuclease Targeted CRISPR Trans Reporter) to reliably detect nucleic acids. (biorxiv.org)
papers communication the author, the Broadbent Institute core member Feng Zhang, Professor,"said SHERLOCK to provide an inexpensive, easy-to-use and sensitive diagnostic method to detect nucleic acid material-this can mean viruses, tumor DNA, and many other targets. (crisprinsider.com)

Various experimental approaches exist for the study of nucleic acid-protein interactions in vitro and in vivo , but the detection of such interactions at the subcellular level remains challenging. (nature.com)
By comparison, five commercial unamplified lateral flow assays that use proprietary antibodies to target the viral nucleocapsid protein exhibit limits of detection of 500 copies/µL, 1000 copies/µL, 2000 copies/µL, 2000 copies/µL, and 20,000 copies/µL. (caltech.edu)
Nucleic acid programmable protein array a just-in-time multiplexed protein expression and purification platform. (nih.gov)
We have developed an innovative in situ protein expression and capture system, namely NAPPA (nucleic acid programmable protein array), where C-terminal tagged proteins are expressed using an in vitro expression system and efficiently captured/purified by antitag antibodies coprinted at each spot. (nih.gov)
Detection of multiple autoantibodies in patients with ankylosing spondylitis using nucleic acid programmable protein arrays. (ox.ac.uk)
Two high density nucleic acid programmable protein arrays expressing a total of 3498 proteins were screened with plasma from 25 patients with AS, 17 with RA, and 25 healthy controls. (ox.ac.uk)
Nucleic acid programmable protein arrays constitute a powerful tool to study autoimmune diseases. (ox.ac.uk)
Module A contained serum samples spiked with cultured dengue virus (DENV) or chikungunya virus (CHIKV) for the detection of nucleic acid and DENV non-structural protein 1 (NS1) antigen. (who.int)

Currently, there are three basic types of tests to determine if an individual has been infected with SARS-CoV-2: viral nucleic acid (RNA) detection, viral antigen detection, and detection of antibodies to the virus. (medscape.com)
Viral tests (nucleic acid or antigen detection tests) are used to assess acute infection, whereas antibody tests provide evidence of prior infection with SARS-CoV-2. (medscape.com)

Comparative evaluation of six nucleic acid amplification kits for SARS-CoV-2 RNA detection. (bvsalud.org)

Relative to proteins, complex nucleic acid-based systems can be designed more readily due to the countable nature of base-pairing interactions and readily available physical models. (caltech.edu)

A method is described for visualizing three nucleic acid sequences simultaneously by in situ hybridization using a new blue immunofluorescent label, amino methyl coumarin acetic acid (AMCA), in combination with green and red fluorescing FITC and TRITC. (nih.gov)
Detection of the rRNA amplification product sequences is achieved using nucleic acid hybridization (HPA). (cdc.gov)

In bulk fluid assays, the dsPNAs had an overall higher fluorescent signal and better sensitivity and specificity than the PNA beacons for pathogen detection. (psu.edu)
This contractor will provide limited coverage for Gastrointestinal Pathogen (GIP) molecular assays identified by multiplex nucleic acid amplification tests (NAATs). (cms.gov)
DESTINA is a diagnostics company, offering innovative Nucleic Acid Testing and molecular diagnostics assays solutions, with a particular emphasis on RNA detection and quantification. (destinagenomics.com)
Target amplification assays are based on the ability of complementary oligonucleotide primers to specifically anneal and allow enzymatic amplification of the target nucleic acid strands. (cdc.gov)

Herein, we propose a nucleic acid testing platform, named MiCaR, which couples a microfluidic device with CRISPR-Cas12a and multiplex recombinase polymerase amplification. (ufluidix.com)
The information in this article contains billing, coding or other guidelines that complement the Local Coverage Determination (LCD) for Foodborne Gastrointestinal Panels Identified by Multiplex Nucleic Acid Amplification (NAATs) (L37364). (cms.gov)

Nucleic acid amplification testing (NAAT, e.g. (cdc.gov)
The "gold standard" for COVID-19 detection is nucleic acid amplification for use of Ag-RDT testing (NAAT) where viral genomes are detected indicating the presence of the virus in an individual. (who.int)

After great efforts and strong determination, Bioteke developed the new product-BioTeke 2019-nCoV variants nucleic acid detection reagents! (bioteke.cn)

Here, we developed a platform with engineered crRNAs and optimized conditions that enabled us to detect DNA, DNA/RNA heteroduplex and methylated DNA with higher sensitivity, achieving a limit of detection of in femtomolar range without any target pre-amplification step. (biorxiv.org)
Time-resolved acquisition of the luminescent signal that occurs upon hybridization of the probe to the target enabled the avoidance of short-lived background fluorescence, markedly enhancing the sensitivity of detection, which was less than 1 pM. (njit.edu)
This is the first comparative study to evaluate CPA and RT-qPCR kits' specificity and sensitivity for SARS-CoV-2 detection , and could serve as a reference for clinical laboratories , thus informing testing protocols amid the rapidly progressing COVID-19 pandemic. (bvsalud.org)
We tested 3 different cell lines with known KRAS mutations and then examined the sensitivity of mutation detection with the cell lines using 189 bp and 92 bp amplicons spanning codons 12 and 13. (nih.gov)
With high sensitivity and precision, Tianlong's SARS-CoV-2 Nucleic Acid Detection Kits can help fast diagnosis and help in pandemic control and prevention. (medtl.net)
Sensitivity for detection exceeds 90%, and specificity is 99% or higher. (medscape.com)

Further applications of this method in prenatal diagnosis for the detection of aneuploidy of the most frequently involved autosomes, as well as for the quantification of gene copy number and mRNA expression, are discussed. (nih.gov)
This method enables detection and quantification of sub-nanomolar cytosolic siRNA release amounts from individual release events with measures of quantitation confidence for each event. (lu.se)
Accurate quantification of cytosolic siRNA, and the establishment of the intracellular dose-response relationships, will aid the development and characterization of novel delivery strategies for nucleic acid therapeutics. (lu.se)

These features of nucleic acids enable us to design novel interaction pathways and functions by providing well-behaved molecular mechanisms. (caltech.edu)
This thesis will first demonstrate the use of these nucleic acid molecular mechanisms in closed-loop genetic circuits and infectious disease testing using cgRNAs and HCR, respectively, then provide updated tools for the nucleic acid design community to exploit the programmable nature of nucleic acids. (caltech.edu)
NUPACK is a growing software suite for the analysis and design of nucleic acid structures, devices, and systems serving the needs of researchers in the fields of nucleic acid nanotechnology, molecular programming, synthetic biology, and across the life sciences. (caltech.edu)
Nucleic acid hybridization is at the core of many amplification-free molecular diagnostics and detection probe configuration is key to diagnostic performance. (psu.edu)
Novel amino-reactive derivatives of lanthanide-based luminescent labels of enhanced brightness and metal retention were synthesized and used for the detection of cDNA oligonucleotides by molecular beacons. (njit.edu)
Detection of acute/recent DENV infection by both molecular (RT-PCR) and serological methods (IgM) was available in 19/24 (79.2%) participating laboratories. (who.int)

Nucleic Acids Res. (nature.com)

In this proof-of-concept study, we report a PCR-free and label-free detection method that has a limit of detection (3 standard deviations) of 15 fmoles of microRNA122, by integrating a dynamic chemical approach for "Single Nucleobase Labelling" with a bead-based platform (Luminex ® ) thereby, in principle, demonstrating the exciting prospect of rapid and accurate profiling of any microRNAs related to diseases and toxicology. (destinagenomics.com)

Zhang added,"this technique shows it in many health care applications potential, including the diagnosis of patient infection and detection to impart drug resistance or cancer-causing mutation, but it can also be used for industrial and agricultural applications, in these applications, the supply chain in the monitoring step to reduce waste and improve safety. (crisprinsider.com)

Nucleic acid nanotechnologies have provided a platform where biologically relevant molecules can be engineered to perform programmable functions. (caltech.edu)

With only one fluorescence probe, MiCaR can simultaneously test up to 30 nucleic acid targets through microfluidic space coding. (ufluidix.com)
In the first study, RNA transcripts encoded for by the ssrA (tmRNA) and lepA (lepA mRNA) genes were evaluated using real-time Nucleic Acid Sequence Based Amplification (NASBA) as potential diagnostic targets for species specific identification of H. influenzae, N. meningitidis and S. pneumoniae. (nuigalway.ie)

Nucleic acid-based diagnostics. (who.int)

The kit is used for qualitative detection of HSV II nucleic acid in male urethral swab samples or female cervical swab samples. (mmtegypt.com)

Diagnosing tuberculosis outside of the lungs High-risk populations for MTB/NTM infection detection include those in intimate contact with a smear-positive patient, those with HIV, those with diabetes, and those taking biologics/immunosuppressants. (capitalbiotechnology.com)
Earlier and improved tuberculosis (TB) case detection - including smear-negative disease, often associated with HIV co-infection - as well as expanded capacity to diagnose multidrug-resistant tuberculosis (MDR-TB) are global priorities for TB control. (bvsalud.org)

Gram stains, cultures, and nucleic acid amplification tests (NAATs) of genital, rectal, conjunctival, pharyngeal secretions and/or other extragenital sites, as applicable, should also be obtained when DGI is suspected. (medscape.com)

The SpectraMax ® QuickDrop ™ Spectrophotometer is a versatile ultraviolet-visible (UV-Vis) micro-volume spectrophotometer exceptional at analyzing nucleic acid samples. (moleculardevices.com)
In this application note, we demonstrate how the QuickDrop spectrophotometer can both quantitatively (concentration) and qualitatively (sample purity) analyze nucleic acid samples with great accuracy and consistency. (moleculardevices.com)
This kit is suitable for the specific detection of pathogenic Helicobacter pylori (Hp) in cat/dog feces samples. (biokeyqpcr.com)
In this new study, they use SHERLOCK to detect lung cancer patient blood samples in a cell-free tumor DNA, while the detection of synthetic zika virus infection and dengue virus, as well as providing other show. (crisprinsider.com)
Module B contained human serum samples for the detection of anti-DENV antibodies. (who.int)

Wooden shafts or calcium alginate swabs may contain substances that inhibit some viruses and nucleic acid detection reactions. (medscape.com)
The researchers are also investigating improved detection of the Zika, dengue and Ebola viruses and predictive biomarkers for cardiovascular disease. (technologynetworks.com)

AnaSpec offers twelve nucleic acid labeling and fluorescent nucleotides. (anaspec.com)

Of these substances, nucleic acids are one of the most routinely measured in life science laboratories. (moleculardevices.com)

La proportion d'infections positives au VRS était plus élevée chez les patients hospitalisés pour une infection respiratoire aiguë que chez les patients en consultation externe souffrant de légers symptômes. (who.int)
The highly infectious phase of acute human immunodeficiency virus (HIV) infection, defined as the interval between the appearance of HIV RNA in plasma and the detection of HIV-1-specific antibodies, contributes disproportionately to HIV transmission ( 1 ). (cdc.gov)
characterized by detection of HCV RNA greater than or equal to 6 months after newly acquired infection. (cdc.gov)

The overall aim of this study was to design, develop, optimise and validate robust, internally controlled nucleic acid based in-vitro amplification methods for the rapid and accurate identification of H. influenzae, N. meningitidis and S. pneumoniae. (nuigalway.ie)

Initial direct viral detection is typically performed using an upper respiratory tract (URT) specimen. (medscape.com)
Quantitative real-time PCR with reverse transcription (RT-qPCR), which measures the quantity of viral RNAs, remains the gold-standard technique for SARS-CoV-2 virus detection 1 . (nature.com)
Le VRS demeure un agent étiologique viral important au Maroc, responsable de syndromes de type grippal et d'infections respiratoires aiguës sévères, en particulier chez les nourrissons. (who.int)

Guangzhou Conducts Nucleic Acid Detection on Personnel from. (referchina.com)
Since April 5, the southern Chinese city of Guangzhou has started conducting nucleic acid detection on all persons who entered the city from high-risk countries, local authorities said Friday. (referchina.com)
As of Thursday, Guangzhou had performed nucleic acid tests on about 4,355 personnel from high-risk countries and put them under health management, the Guangdong provincial health commission told a press briefing. (referchina.com)
The shelter is used with Suzhou Antai Airtech Biosafety cabinet, it has been used for the first-line anti-epidemic in Guangzhou and Foshan districts to conduct nucleic acid testing. (airtechsat.com)
It's Report that the 'Huoyan' laboratory in Guangzhou has a daily nucleic acid detection volume of up to 150,000 tubes, It is the highest daily detection the monomer laboratory in China, Suzhou Antai Airtech Biosafety Cabinet also used in the laboratory. (airtechsat.com)

We have developed a real-time NASBA procedure for detection of piscine nodaviruses, which have emerged as major pathogens of marine fish. (int-res.com)

Based on the detection of cell culture-derived nodavirus, and a synthetic RNA target, the real-time NASBA procedure was approximately 100-fold more sensitive than single-tube RT-PCR. (int-res.com)

SARS-CoV-2 detection was simultaneously performed on each sample using six nucleic acid amplification kits. (bvsalud.org)

Electrochemical Impedance Spectroscopy (EIS) was also performed on the modified electrode as another method of NA detection. (dcu.ie)
Inductively-coupled plasma mass spectrometry (ICP-MS) based method that allows for the comprehensive in-line characterization of EINPs with a single technique and at single-particle level of detection. (epfl.ch)
Tianlong provides integrated solution of SARS-CoV-2 Nucleic Acid Amplification Tests based on the PCR method for diagnosing COVID-19 patients. (medtl.net)

Totally 4 sets of 'Falcon' air-film shelter laboratories daily nucleic acid detection capacity can exceed 120,000 tubes. (airtechsat.com)

The CRISPR/Cas12a RNA-guided complexes have a tremendous potential for nucleic acid detection due to its ability to indiscriminately cleave ssDNA once bound to a target DNA. (biorxiv.org)
However, the current CRISPR/Cas12a systems are limited to detecting DNA in a picomolar detection limit without an amplification step. (biorxiv.org)
Microfluidic space coding for multiplexed nucleic acid detection via CRISPR-Cas12a and recombinase polymerase amplification. (ufluidix.com)

Perform a culture or nonculture detection test for N gonorrhoeae on endocervical, urethral, pharyngeal, or rectal discharge. (medscape.com)

Direct probe labeling in a 10 min reaction, 1 h from hybridization to detection with ECL Direct, Hybond N+, and Hyperfilm ECL. (fishersci.com)
We then used the hybridization chain reaction mechanism to develop an adaptable and sensitive test for the detection of SARS-CoV-2. (caltech.edu)
Within a detection limit of 42 RNA copies per reaction, SHERLOCK was 100% specific and 100% sensitive with a fluorescence readout, and 100% specific and 97% sensitive with a lateral-flow readout. (nature.com)

Men who have sex with men were invited to complete a short questionnaire on mouthwash use and they were also tested for pharyngeal gonorrhoea by nucleic acid amplification test. (nih.gov)
Although the proportion of daily use of mouthwash increased with age, and pharyngeal gonorrhoea detection decreased with age, the association between self-reported mouthwash use and pharyngeal gonorrhoea detection by nucleic acid amplification test was not statistically significant. (nih.gov)
4) Nucleic acid amplification test. (mmtegypt.com)

Presently, culture remains the gold standard for the diagnosis of bacterial meningitis, however, this is limited in its ability to rapidly and accurately diagnose and nucleic acid based diagnostic methods are more sensitive and specific for the diagnosis of this fatal disease. (nuigalway.ie)

PCR) is preferred for adenovirus detection and may be performed on respiratory specimens, stool or rectal swabs, or blood. (cdc.gov)

Anatomy12

Organisms12

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Assay7

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