Detection of caspase 3. Medical search. Frequent questions

Cytochrome c release, caspase-3 activation, and apoptotic chromatin condensation were unchanged in all mutant fibroblasts. (nature.com)
3 Pro-apoptotic proteins, such as cytochrome c and second mitochondria-derived activator of caspase (SMAC), are released from mitochondria into the cytosol where they participate in the activation of execution caspases. (nature.com)
4 Although the mechanisms of caspase activation in the cytosol are well characterized, the steps that control cytochrome c release from mitochondria and thereby activate the apoptosis program have remained largely elusive. (nature.com)
Western blotting and reverse transcriptase PCR were used to determine the expression levels of apoptosis-related proteins and genes, including bcl-2 , bax , cytochrome c , and capase-3 . (biomedcentral.com)
Radiation also dramatically decreased the protein and mRNA expression of bcl-2 and increased that of bax, cytochrome c, and capase-3. (biomedcentral.com)
Excessive oxidative stress alters the expression levels of apoptosis-related genes and triggers sperm apoptosis through bcl-2, bax, cytochrome c and caspase-3 signaling pathways. (biomedcentral.com)
Moreover, the detection of the anti-apoptotic protein Bcl-2 and apoptotic proteins BAX, cytochrome c, as well as the activating effector caspase-3 revealed that T. pyogenes induced apoptosis of BEECs, which was also confirmed by DAPI staining to observe the morphological changes of the nuclei of cell apoptosis and by TUNEL staining to locate the cells undergoing apoptosis. (preprints.org)
Mitochondrial regulation of apoptosis further downstream was investigated, showing changes in the mitochondrial membrane potential, cytochrome c release into the cytoplasm, and enhanced activation of cleaved caspase-3 and Poly ADP-ribose polymerase (PARP). (hindawi.com)
Protein and gene expression of apoptotic signaling pathway-related proteins such as caspase-3, cytochrome c, and PARP were determined to provide evidence for the mechanisms of action of Tan IIA in the treatment of psoriasis. (hindawi.com)

Cell apoptosis was measured by flow cytometry, TUNEL assay, and detection of caspase 3 and Bcl-2. (medscimonit.com)
BD Biosciences provides you a choice of flow cytometry-based kits for the detection of early apoptotic cells. (bdbiosciences.com)

For instance, caspase-3 indirectly activates CAD (caspase-activated deoxyribonuclease) while inactivating ICAD (inhibitor of caspase-activated deoxyribonuclease), which contributes to chromatin fragmentation in nucleosome units. (caltagmedsystems.co.uk)
They were divided into blank group (with medium only), 3-MA (autophagy inhibitor) pretreatment group (with 2 ml of 5 mM 3-MA medium), H2O2 Intervention group (add 2ml medium containing 0.05mM H2O2), H2O2+3-MA treatment group (add 2ml medium containing 5mM 3-MA, then add 2ml medium containing 0.05mM H2O2). (researchsquare.com)
Interestingly, while GT1b enhanced caspase-3 activity, DEVD, a caspase-3 inhibitor, failed to rescue DA neuronal death. (biomedcentral.com)
Moreover, a GSK-3β specific inhibitor, L803-mt, attenuated tau phosphorylation and rescued DA neurons from cell death in mesencephalic cultures. (biomedcentral.com)
Apoptosis was partially blocked by the caspase-3 inhibitor Ac-DEVD-CHO. (hindawi.com)

Only those wells that contain Rat CASP14, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. (caslab.com)
The fluorescence intensity (Ex / Em = 400 / 490nm) is directly proportional to the activity of caspase-3. (creative-biolabs.com)
Cell death was determined by Annexin V/PI double staining of fluorescence activated cell sorting, lactate dehydrogenase release and caspase-3 activity. (biomedcentral.com)
upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (lmax = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader. (cephamls.com)

CellEvent Caspase-3/7 Green and CellEvent Caspase-3/7 Red detection reagents are novel fluorogenic substrates for activated caspase-3/7 in live cells that can be fixed and multiplexed with antibody detection workflows such as immunofluorescence (IF) and immunocytochemistry (ICC). (thermofisher.com)
pNA (4-nitroaniline)-derived caspase substrates are widely used for the colorimetric detection of various caspase activities. (eurogentec.com)
Active caspase identifies multiple molecules as substrates and cleaves them, leading to biological events associated with apoptosis. (caltagmedsystems.co.uk)
Caspase also recognises several structural proteins as substrates, leading to cleavage that is associated with distinct morphological changes in apoptotic cells, including chromatin condensation, nucleus fragmentation, and cytoplasmic integrity. (caltagmedsystems.co.uk)
In addition, BD Biosciences offers caspase inhibitors that can be used as controls and substrates. (bdbiosciences.com)

The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate IETD-pNA. (sigmaaldrich.com)
The Caspase-8 substrate and pNA standard are especially light sensitive. (sigmaaldrich.com)
Enhanced apoptosis was identified following a 48‑h incubation with 6‑OAP (0‑10 µM) that induced a dose‑dependent decrease in pro‑casp‑3 and the cleavage of its substrate, anti‑poly (ADP-ribose) polymerase (PARP). (spandidos-publications.com)
MBLI 's APOPCYTO Caspase-3,8,9 Colorimetric Assay Kit detects caspase activity in cell extract with LEHD-pNA as a substrate, in which LEHD sequence is recognised by each active caspase selectively. (caltagmedsystems.co.uk)
Immunoblot analysis revealed that GT1b inactivates Akt through dephosphorylation at both Ser473 and Thr308, subsequent dephosphorylation of GSK-3β, a substrate of Akt, and hyperphosphorylation of tau, downstream of GSK-3β. (biomedcentral.com)
In the assay, a specific substrate (N-Ac-DEVD-AFC) is cleaved by active caspase-3 to form a highly fluorescent product. (creative-biolabs.com)
The assay is based on detection of cleavage of substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). (cephamls.com)

XPO1, the main nuclear export protein, transports cargo proteins containing nuclear localization signals out of the nucleus ( 1-3 ). (aacrjournals.org)
E-cadherin immunofluorescent double labeling can also be used with activated caspase 3 or â-catenin immunofluorescence to localize the expression of these proteins in damaged airways. (cdc.gov)
The HA tag is not suitable for detection or purification of proteins from apoptotic cells since it is cleaved by Caspase-3 and / or Caspase-7 after its sequence DVPD, causing it to lose its immunoreactivity. (eurogentec.com)
C1QBP significantly increased ROS level, apoptosis, and the expression of apoptotic proteins such as cleaved caspase-3 and bax/bcl2 via regulating XDH. (medsci.org)

Our data provide novel evidence that a Akt/GSK-3β/tau-dependent, but not caspase-3 signaling pathway plays a pivotal role in GT1b-mediated neurotoxic actions on mesencephalic DA neurons. (biomedcentral.com)
There was also no translocation of apoptosis-inducing factor (AIF) from mitochondria to the nucleus in apoptotic keratinocytes, indicating Tan IIA-induced apoptosis occurs mainly through the caspase pathway. (hindawi.com)

Toxicol Lett 159(3):261-271. (cdc.gov)

Immunohistochemical analysis was performed for detection of WNVCp-DJY protein expression in mouse brain injected with pcDNA3.1 or pcWNV-Cp-DJY as detected with horseradish peroxidase (HRP) (e,f, respectively). (cdc.gov)
Immunohistochemical studies were performed for detection of WNV-Cp-DJY protein expression in mouse brain injected with pcWNV-Cp-DJY or pcDNA3.1 as detected by fluorescein isothiocynate stain (h, i, and j, k, respectively). (cdc.gov)
The aim of the presented study was to perform the immunohistochemical detection of endothelial (eNOS) and inducible (iNOS) isoform of nitric oxide synthase in the adenomatous and hyperplastic parathyroid gland in relation to the apoptotic process. (nel.edu)
Immunohistochemical analysis of paraffin-embedded Jurkat cell pellets, untreated (left) or etoposide-treated (right), using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb on SignalSlide ® Cleaved Caspase-3 (Asp175) IHC Controls #8104. (cellsignal.jp)

Activation of caspase-3 is an essential event during apoptosis, making these optimized reagents for analysis of apoptotic cells. (thermofisher.com)
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. (sigmaaldrich.com)
Whereas in the acute phase of the disease immunoregulatory processes prevail in the hippocampus and the cortex, we observed a strong activation of neurogenic processes in the hippocampal dentate gyrus, both by gene expression and immunohistology starting as early as 3 days after infection. (biomedcentral.com)
In this study, we demonstrate for the first time that GT1b neurotoxicity is accompanied by inactivation of Akt, activation of GSK-3β and increase in tau phosphorylation in mesencephalic DA neurons, both in vivo and in vitro . (biomedcentral.com)
Organic solvent-induced proximal tubular cell toxicity via caspase-3 activation. (cdc.gov)
43] Additionally, knockdown of slingshot 2 (SSH2) serine phosphatase was demonstrated to induce caspase 3/7 activation that can trigger apoptosis in human carcinoma cells with a FLCN mutation. (medscape.com)

CellEvent Caspase-3/7 Red Detection Reagent can also be used to detect apoptosis in GFP expressing cells. (thermofisher.com)
Identification and quantification of caspase-3 activity in apoptotic cells. (bdbiosciences.com)
Flow cytometric enumeration of damaged cells through the detection of micronuclei within each cell. (bdbiosciences.com)
BD Biosciences provides a variety of reagents for the identification and quantification of caspase-3 activity in apoptotic cells. (bdbiosciences.com)
Under experimental normoxic condition (21 % O 2 ), deferasirox (DFX) used at 10 μM for 3 days reduced proliferation, led cell cycle arrest in S and G2-M phases and induced cytotoxicity and apoptosis in U251 and U87 GBM cells. (biomedcentral.com)
Immunofluorescent double-labeling with e-cadherin can be used with immunofluorescent detection of podoplanin, a lymphatic endothelial cell marker that is also expressed by alveolar type I cells in the lung. (cdc.gov)
Lead-induced apoptosis in PC 12 cells: involvement of p53, Bcl-2 family and caspase-3. (who.int)

In order to quantitatively determine the apoptotic target caspase-3/7 activity, and use HTS to screen apoptosis inducers and inhibitors. (creative-biolabs.com)

The mechanism of 6‑OAP cytotoxicity in all cell lines was associated with the induction of apoptosis with the presence of cleaved caspase‑3 and PARP. (spandidos-publications.com)

Description: A polyclonal antibody for detection of Caspase-1 from Human, Mouse, Rat. (bd-ibr.org)
This Caspase-1 antibody is for WB, IHC-P, ELISA. (bd-ibr.org)
Then a biotinylated detection antibody specific for Rat CASP14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. (caslab.com)

Our data further confirm that caspase-3 is not a major effector of DA neuronal death in GT1b neurotoxicity. (biomedcentral.com)

The CPP32/Caspase-3 Fluorometric Protease Assay Kit provides a simple and convenient means for assaying the DEVD-dependent caspase activity. (cephamls.com)

Caspase 8 Colorimetric Activity Assay Kit, IETD Caspase-8 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence IETD. (sigmaaldrich.com)
Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-8 activity. (sigmaaldrich.com)
The detection of caspase activity is crucial for apoptosis research. (caltagmedsystems.co.uk)
Creative Biolabs' Caspase-3/7 Activity Assay Kit provides a convenient method to measure caspase-3 activity in biological samples. (creative-biolabs.com)
Here, we present caspase 3/7 activity as a read-out for monitoring the replication of SARS-CoV-2 isolates from different variants, including a remdesivir-resistant strain, and of other coronaviruses in a broad range of cell culture models, independently of cytopathogenic effect formation. (biorxiv.org)
Evidence of apoptosis at the cellular level will be determined by measuring caspase-3/7 activity and DNA fragmentation. (georgiasouthern.edu)
Data collection is on-going for caspase-3/7 activity and RT-qPCR results. (georgiasouthern.edu)

Cleavage of pNA peptides by caspases generates pNA that is monitored colorimetrically at ~405 nm. (eurogentec.com)

The inhibition of the tumor growth was accompanied with changes in proliferation and apoptosis as determined by reduced Ki-67 expression, increased expression of p21WAF/Cip1, cleaved caspase 3 and caspase-cleaved keratin 18. (lu.se)

Caspase belongs to the cysteine aspartic acid-specific protease family, which gets activated by various signals such as death receptor ligation, DNA damage, serum starvation, and stresses. (caltagmedsystems.co.uk)
The aim of this study was to evaluate serum levels of caspase-3, caspase-8, and caspase-9 in women with ovarian cancer. (mdpi.com)

3' of this transgene is a WPRE sequence and an hGH polyA signal sequence (pA). (cdc.gov)

Recent evidence has indicated that the vascular endothelial injury induced by hyperglycemia is associated with the enhanced nitrooxidative stress both in vivo and in vitro [ 3 - 6 ]. (hindawi.com)

up-regulated Beclin1, mTOR, LC3A/B and Cleaved caspase-3 protein expression, and down-regulated p-mTOR Protein expression level. (researchsquare.com)
up-regulated the protein expression of Beclin1, mTOR, LC3A/B and Cleaved caspase-3 Down-regulate the expression of p-mTOR protein. (researchsquare.com)

APOPCYTO Caspase-3,8,9 Colorimetric Assay Kits are composed of reagents necessary for the detection, which includes Cell Lysis buffer, Reaction buffer and so on. (caltagmedsystems.co.uk)
A nitric oxide (NO) detection kit was used to determine the concentration of NO. Western blotting analysis was performed to determine the levels of intercellular cell adhesion molecule (ICAM)‑1, vascular cell adhesion molecule (VCAM)‑1, endothelin‑1, caspase‑3, Bax, Bcl‑2, Toll‑like receptor 4 (TLR4), p65 and p‑p65. (spandidos-publications.com)
Compared with the ox‑LDL group, S/V treatment significantly increased the cell viability, NO concentration and Bcl‑2 expression, decreased the levels of IL‑1β, IL‑6 and TNF‑α and reduced the expressions of MALAT1, ICAM‑1, VCAM‑1, cleaved‑caspase‑3, Bax, TLR4 and p‑p65. (spandidos-publications.com)

With the development of research, the currently recognized mechanisms include lipid metabolism disorder, inflammatory response, oxidative stress and so on ( 3 , 4 ). (spandidos-publications.com)
Iron chelation therapy has already had a significant clinical impact on diseases other than cancer, primarily to treat iron-overload diseases but also to treat oxidative stress in neurodegenerative diseases [ 3 , 4 ]. (biomedcentral.com)

In conclusion, 6‑OAP‑induced apoptosis is caspase‑dependent. (spandidos-publications.com)

Reagents for western blot- and immunohistochemistry-based methods for apoptosis detection. (bdbiosciences.com)

In line with this theory, applying iron chelators that bind very tightly to iron thereby promoting its excretion and subsequent depletion in biological systems should be of major interest [ 3 ]. (biomedcentral.com)

The major events in the regulation of the host response on a transcriptional level occur within the first 3 days after infection. (biomedcentral.com)

the expression of specific CNS enzyme (enolase), proinflammatory cytokines MIF and apoptotic marker caspase-3 in the umbilical blood of infants delivered for Covid positive mothers. (who.int)

An impaired tumor angiogenesis was also demonstrated by detection of increased levels of VEGF and reduced vessel size. (lu.se)

AnaSpec offers labeling and detection products such as biotin and streptavidin derivatives, fluorescent and non-fluorescent dyes. (anaspec.com)

Primers used to detect integration of the 5' arm (F1, R1) and the 3' arm (F3, R2) only detect homologously recombined insertion events into the genome. (cdc.gov)

Study of methanol extract of aerial parts yielded 3,4-dihydroverbenalin and daucosterol. (stuartxchange.org)

The onset of RCC is hidden, and 16% of patients have metastasized at first diagnosis with a 5-year survival of 9-12%[ 3 ]. (medsci.org)
In China, patients with psoriasis often turn to alternative and complementary treatments, which are considered effective and safe [ 3 ]. (hindawi.com)

The hypoxia-sensitive striped bass ( Morone saxatilis) and the hypoxia-tolerant Nile tilapia ( Oreochromis niloticus) were sampled at four key time points: 1) prior to hypoxia exposure (normoxic control), 2) after four hours at the species' specific Pcrit (hypoxia), 3) immediately upon return to normoxia (reperfusion), and 4) after four hours at normoxia (recovery). (georgiasouthern.edu)
This suggests both species of fish did not experience hypoxia long enough to result in the detection of the final stages of apoptosis. (georgiasouthern.edu)

Anatomy18

Organisms8

Diseases5

Chemicals and Drugs105

Analytical, Diagnostic and Therapeutic Techniques and Equipment70

Psychiatry and Psychology2

Phenomena and Processes42

Disciplines and Occupations6

Technology, Industry, Agriculture2

Information Science9

Health Care11

Cytochrome9

Flow cytometry2

Inhibitor5

Fluorescence4

Substrates5

Substrate7

Proteins4

Pathway2

Toxicol Lett1

Immunohistochemical4

Activation6

Cells7

Apoptosis inducers1

PARP1

Antibody3

Effector1

DEVD1

Activity7

Cleavage1

Proliferation1

Serum2

Sequence1

Endothelial1

Protein2

Cell3

Oxidative stress2

Conclusion1

Reagents1

Biological1

Regulation1

Marker1

Tumor1

Fluorescent1

Detect1

Extract1

Patients2

Species2