Detection determination in dried blood. Medical search. Frequent questions

Schroder TH, Quay TA, Lamers Y. Methylmalonic acid quantified in dried blood spots provides a precise, valid, and stable measure of functional vitamin B-12 status in healthy women . (kcl.ac.uk)
Carducci C, Birarelli M, Santagata P, Leuzzi V, Carducci C, Antonozzi I. Automated high-performance liquid chromatographic method for the determination of guanidinoacetic acid in dried blood spots: a tool for early diagnosis of guanidinoacetate methyltransferase deficiency. (gaa-science.com)
Background: Dried blood spots (DBS) collected on filter paper have eased the difficulty of blood collection in resource-limited settings. (blogspot.com)
Although the homologues of PEth could be isolated from whole blood, less invasive dried blood spots (DBS) also provides reliable information. (nofor.org)

blood or urine for establishing exposure to drugs has become The last decade has also seen a signiﬁ cant development in the a signiﬁ cant direction in clinical and forensic toxicology.1 understanding of the target drugs and their pharmacokinetics These alternative specimens include hair, sweat and oral in oral ﬂ uid. (nomedicalcare.com)
Oral ﬂ uid has been seen as a non-invasive alternative to drugs and what concentrations need to be targeted, and also blood but also as an alternative to urine when substitution or how these concentrations may or may not relate to blood adulteration is suspected. (nomedicalcare.com)
ﬂ uid cannot be seen as a substitute for blood or urine drug In addition, the use of initial screening cartridges or devices testing. (nomedicalcare.com)
Purge-and-trap collection is well suited to biological samples such as blood or urine that are readily soluble in water (Ashley et al. (cdc.gov)
Whilst the use of cannabinoids is banned in equine sports, there is limited information available concerning CBD detection times in blood or urine. (researchgate.net)
AbstractMineralization procedures for blood and urine suitable for the determination of arsenic by Hydride Generation Atomic Absorption Spectrometry (HGAAS) are studied on model samples, and the results are utilized in biological monitoring investigations. (archive.org)
Two preparation procedures for urine give As recoveries close to 100% by HGAAS: a) dry ashing at 420°C with Mg(NO3)2 on a hot plate, and b) microwave oven decomposition with (NH4)2S2O8. (archive.org)
For instance, EtG and EtS are the most common used biomarkers for clinical studies and forensic laboratories, although their detection time in both urine and serum is short [3]. (nofor.org)
Gas chromatographic screening for Zopiclone presence in blood and urine. (medline.ru)
The purpose of our study was to identify zopiclone and its alkaline hydrolysis products by TLC, GC-FID and GC-ECD in blood and urine samples. (medline.ru)
The main purpose of our study was to detect native zopiclone, its decomposition products and 2-amino-5-chloropyridine in blood and urine by means thin-layer chromatography and gas chromatography (FID and ECD). (medline.ru)
The isolation of the anylate was performed by liquid/liquid extraction of urine and blood samples (5,0 ml) with dichloromethane-acetone (4:1,v/v) at pH 8,0 (two times with 10,0 ml, extraction time 5 min. (medline.ru)
Aliquots of blood, urine samples and zopiclone hydrolysis products were first analyzed by thin-layer chromatography in solvent system benzene-1,4-dioxane-conc. (medline.ru)

High performance liquid chromatographic methods have been applied for the quantitative determination of the drugs and their metabolites which has enabled most of the methods used/ developed to be sensitive and specific with low limits of detection. (mak.ac.ug)
Chromatographic separations were performed using a gradient program with a mixture of water (0.05% formic acid), as mobile phase A and acetonitrile (0.05% formic acid), as mobile phase B. Analytes quantification was performed by electro-spray ionisation-single quadrupole mass spectrometry using the selected ion recording (SIR) detection mode. (unito.it)
Our technical focus and capabilities include a range of extraction approaches, separation methods (solid and liquid phase), detection techniques, chromatographic techniques, spectroscopy (including accurate mass spectrometry, spectrofluorimetry, surface enhanced Raman spectroscopy and energy dispersive spectroscopy), and chemometric techniques for data handling and optimization. (bournemouth.ac.uk)

Dried capillary blood samples are an excellent alternative to venous blood samples and can be analysed subsequently in the lab, e.g. for antibodies against SARS-CoV-2. (euroimmun.de)
Baseline assessments, prior to thiamine administration, include a physical examination, echocardiogram and venous blood draw for the determination of thiamine biomarkers. (bmj.com)
Briefly, the method was consisted of a 4 mL venous blood epileptic seizures, primarily and secondarily generalized tonic-clonic sample collected in heparinized tube previously to the morning LTG seizures, absence seizures and the drop-attacks associated to Lennox- dose ingestion (trough level). (bvs.br)

2008). Thus, the aim high performance liquid chromatography with ultraviolet of the study is to evaluate the association between LTG detection (HPLC-UV). (bvsalud.org)

Detection of antibodies to Bartonella vinsonii subsp. (tamu.edu)
Detection of antibodies to Neospora caninum in canine serum by competitive ELISA. (tamu.edu)
Detection of antibodies to Babesia canis by indirect fluorescent antibody test. (tamu.edu)
Blood samples from the participants were assayed for total and S. haematobium (Sh13)-specific antibodies before and 2 years after annual rounds of treatment. (edctpalumninetwork.org)
In seroconverters from the CAPRISA 004 tenofovir gel trial, we previously reported significantly higher detection and titres of HIV-specific binding antibodies in the plasma and genital tract (GT) that distinguished the tenofovir from the placebo arm. (edctpalumninetwork.org)
Detection of antibodies to Corynebacterium pseudotuberculosis, the causative agent of caseous lymphadenitis, by synergistic hemolysis inhibition. (tamu.edu)

The differential diagnosis for elevated galactose concentrations in blood on a newborn screening result can include other disorders of galactose metabolism, including galactokinase deficiency and galactose epimerase deficiency. (wikipedia.org)
Tetrahydrocannabinol (THC), the major species present from cannabis use, displays similar concentrations in oral ﬂ uid compared to blood in the elimination phase. (nomedicalcare.com)

Biochemical genetic testing and newborn screening are essential laboratory services for the screening, detection, diagnosis, and monitoring of inborn errors of metabolism or inherited metabolic disorders. (cdc.gov)
The aim of this study was to test the use of IP-10 detection in dried plasma from contact studies individuals (contacts of smear positive patients), by comparing it with IP-10 and IFN-γ detection in direct plasma, to establish IP-10 detection in DPS as a useful assay for LTBI diagnosis. (uab.cat)
MSTA is not indicated for the immunization , diagnosis, or treatment of mumps virus infection, or determination of immune status to mumps virus. (rxlist.com)
In the transplant population, antigen assays or PCR is used (sometimes in conjunction with cytopathology) for diagnosis and treatment determinations. (medscape.com)

The proposed method was evaluated by carrying out replicate ethylene glycol determinations on fortified whole blood samples at the levels of 12.5 mM, 20.0 mM, 31.2 mM, 100 mM and 200 mM comparable to commonly reported blood levels in intoxications. (rsc.org)
This methodology enables detection of putative metabolites from biological samples and simultaneous quantification of the pattern and extent of isotope labeling. (acs.org)
EUROIMMUN provides a CE-marked blood collection card , which can be used for the collection and shipment of dried capillary blood samples. (euroimmun.de)
According to the US Department of Transportation (DOT), the International Air Transporter Association (IATA) and the World Health Organisation (WHO), dried blood samples are exempt medical samples that can be sent by mail with the packaging materials included in the product. (euroimmun.de)
Whole blood samples were collected from 80 subjects: 12 with active tuberculosis (TB), and 68 from contact studies. (uab.cat)
Brief summaries of the methods available for extraction and detection of these compounds in biological and environmental samples are provided below. (cdc.gov)
Methods: A total of 182 blood samples from C. dromedarius from nomadic and dairy farms were evaluated using STDM, serological (CATT/T. evansi) and molecular (ITS1-PCR) methods. (researchgate.net)
Stool samples from an outbreak that are submitted to CDC for detection of viral pathology should be collected in bulk from 10 ill persons during their first 48 hours of illness, while feces are still liquid, and should be stored at 4 C (not frozen). (cdc.gov)
This thesis deals with the development and validation of analytical methods that are field-adapted and their application in the determination of antimalarial drug levels in biological samples. (mak.ac.ug)
The determination of antimalarial drugs in biological samples from Ugandan healthy volunteers and patients is discussed. (mak.ac.ug)
For blood samples, As recoveries by HGAAS range between 95 and 108% for all species when using dry ashing after a pretreatment of samples with HNO3 and H2O2 in a microwave oven. (archive.org)
For other As species in spiked blood samples, recoveries of less than 20% As are found. (archive.org)
Labsystems Diagnostics' Neonatal IRT FEIA is a fluorometric enzyme immunoassay for the quantitative determination of human immuno reactive trypsinogen (IRT) from blood samples dried on filter paper as a primary test for screening of babies for cystic fibrosis (CF). (trivitronhealthcare.co.in)
Rapid Detection of Amitriptyline in Dried Blood and Dried Saliva Samples with Surface-Enhanced Raman Spectroscopy. (bournemouth.ac.uk)
During the hospital stay, maternal blood and breast-milk samples and diet, health, anthropometric and socio-demographic information will be collected for mother-child pairs. (bmj.com)

Detection of tetrahydrocannabinol (THC), cannabidiol (CBD), their major metabolites, and a variety of synthetic cannabinoids by liquid chromatography/mass spectrometry. (tamu.edu)
Field adapted analytical methods for determining therapeutic levels of amodiaquine, lumefantrine and their metabolites from whole blood, dried on filter papers have been developed and validated. (mak.ac.ug)

A number of devices are suitable for detection and quantification of dibromochloro methane and bromoform as they emerge from the GC, including flame ionization detection (GC/FID), halogen-sensitive detection (GC/HSD) or electron-capture detection (GC/ECD). (cdc.gov)
A HPLC-MS method for the simultaneous quantification of fourteen antiretroviral agents in peripheral blood mononuclear cell of HIV infected patients optimized using medium corpuscular volume evaluation. (unito.it)

Conﬁ rmatory techniques are largely based on mass spectrometry (MS) with an emphasis on Liquid Chromatography-Mass Spectrometry (LC-MS), due to low sample volumes and the low detection limits required. (nomedicalcare.com)
Additionally, analytical methods are included that modify previously used methods to obtain lower detection limits and/or to improve accuracy and precision. (cdc.gov)
Precision and detection limits obtained by both techniques are evaluated as well. (archive.org)

The method, based on reversed-phase (RP)-HPLC, precolumn derivatisation with benzoin and fluorescence detection, has shown good precision and sensitivity and requires only minimal sample handling. (gaa-science.com)

DBS collected on M-TFN had the highest genotyping efficiency (100%) compared to W-903 and A-226 (91.7%), and appear more sensitive in detecting major HIVDR mutations.Conclusions: DBS collected on A-226 and M-TFN filter papers performed similarly to W-903 for quantitative VL analysis and HIVDR detection. (blogspot.com)

Analytical methods for the determination of bromoform and dibromochloromethane in biological materials are summarized in Table 7-1. (cdc.gov)
The purpose of this review is to provide a complete update of the various analytical methods for virus detection, such as molecular, immunological, sensor-based and other detection assays, along with the advantages and limitations of these strategies. (frontiersin.org)
BU ASRG is the hub of analytical science at the university for both research and commercial projects, providing state-of-the-art analytical instrumentation and technical expertise for sample extraction, separation, detection and characterisation. (bournemouth.ac.uk)

bone marrow in EDTA, 1 mL peripheral blood in EDTA, air-dried slides. (tamu.edu)
Submission of a fresh peripheral blood smear is also recommended. (tamu.edu)
A sensitive and accurate high performance liquid chromatography-mass spectrometric (HPLC-MS) method for the intracellular determination of 14 antiretroviral drugs in peripheral blood mononuclear cells (PBMCs) for HIV+ patients was validated. (unito.it)

Health-care facilities involved in the detection of rotavirus or the other viral agents of diarrhea can participate. (cdc.gov)
Comparison of Ahlstrom Grade 226, Munktell TFN, and Whatman 903 filter papers for Dried Blood Spot (DBS) specimen collection and subsequent HIV-1 viral load and drug-resistance genotyping analysis. (blogspot.com)
This situation has created an alarming public health emergency of international concern requiring the detection of this life-threatening viral candidate due to increased cases of newborn microcephaly associated with ZIKV infection. (frontiersin.org)
Trevor Shoemaker is a senior epidemiologists with the CDC's Viral Special Pathogens Branch, he currently focuses on enhancing surveillance and outbreak detection for viral hemorrhagic fevers internationally. (cdc.gov)

Mature miRNAs can be detected in blood plasma, serum and also in a wide variety of biological fluids. (degruyter.com)

Detection of arsenic salts by the qualitative Reinsch test. (tamu.edu)

Detection of Dermatophilus congolensis, the causative agent of dermatophilus, by Giemsa stain. (tamu.edu)
These tests are useful in detection of difficult-to-culture pathogens such as norovirus (causative agent for gastroenteritis), and mycobacterium tuberculosis (TB) in standard conventional diagnostics testing. (clpmag.com)

Although these biosensors are currently among the less popular tools for human disease detection, they have become useful tools for the screening and detection of pathogenic bacteria, fungi, and viruses because of their versatile advantageous features compared to other sensing devices. (frontiersin.org)

This review summarizes the currently available methods employed for the rapid detection of ZIKV and suggests an innovative approach involving the application of a liposome-based hypothesis for the development of new strategies for ZIKV detection and their use as effective biomedicinal tools. (frontiersin.org)
Considering the calamity of ZIKV infection, there is an urgent need to develop rapid detection methods for ZIKV along with DENV, which shares common clinical symptoms with ZIKV. (frontiersin.org)

We hypothesized that higher HIV-specific antibody titres and detection reflected corresponding increased antibody-dependent neutrophil-mediated phagocytosis (ADNP) and NK-cell-activated antibody-dependent cellular cytotoxic (ADCC) activities. (edctpalumninetwork.org)
IRT is eluted from dried blood disks simultaneously forming a sandwich between the solid phase, coated with an antibody that recognizes Trypsinogen-1 (IRT-1) and a second antibody, which is labeled with horseradish peroxidase (HRP). (trivitronhealthcare.co.in)

Protoporphyrin is extracted from EDTA whole blood into a 2:1 (v/v) mixture of ethyl acetate acetic acid, then back extracted into diluted hydrochloric acid. (cdc.gov)

Enzyme assays are commonly done using fluorometric detection or older radioactively labeled substrates. (wikipedia.org)

After a correction for the individual hematocrit is made, the final concentration of protoporphyrin in a specimen is expressed as micrograms per deciliter (ug/dL) of packed red blood cells (RBC). (cdc.gov)

It has several advantages over conventional chromogenic reagents in the colorimetric determination of hydrogen peroxide activity. (whdsbio.cn)

In particular, kits are now designed for on-site drug detection without the need for sophisticated laboratory The introduction of LC-MS as a routine laboratory technique screening equipment. (nomedicalcare.com)
For dried plasma IP-10 determination, two 25 µl plasma drops were dried in Whatman903 filter paper and sent by mail to the laboratory. (uab.cat)

Although it is hard to detect the ethanol in biological matrices after 10-12 hours after consumption [3], other biomarkers produced in the body might be more convenient for detection of alcohol. (nofor.org)

In hemorrhagic infarction, the extravasated red blood cells promote active inflammation and participate in the formation of fat deposition within the infarction zone. (nature.com)

However, for specific substrates, testing different types of new Trinder 's reagents is necessary to develop the best detection system. (whdsbio.cn)
Fast Black K /1 N NaOH and Dragendorff/Jodplatinate reagents can be used for zopiclone and its alkaline hydrolysis products detection. (medline.ru)

Although it is found in some parts of the body, antemortem forensic cases require different matrices for detection of PEth. (nofor.org)

The attributable fraction (AF) of influenza virus detection to illness has not been described for patients in different age groups or with different HIV infection statuses. (cdc.gov)
found the relative risk at -70 °C) were transferred in dry ice to the of developing liver failure in HCV infection laboratories of Sorin Biomedica, Saluggia, increased 21-fold after HIV infection [ 7 ]. (who.int)

In most regions, galactosemia is diagnosed as a result of newborn screening, most commonly by determining the concentration of galactose in a dried blood spot. (wikipedia.org)
Dried blood has been used as a sample material for in vitro diagnostic analysis since the mid 1960s, especially in newborn screening and, over the past decades, has become a standard in this field. (euroimmun.de)

Detection of anabolic steroids by liquid chromatography/mass spectrometry. (tamu.edu)
Detection of performance enhancing beta-agonist drugs by liquid chromatography / mass spectrometry. (tamu.edu)

A simple and rapid method involving thermal desorption gas chromatography (TD-GC) with flame ionisation detection has been successfully developed for the determination of ethylene glycol in whole blood. (rsc.org)
A new automated method for the assay of guanidinoacetic acid (GAA) in dried blood spot (DBS) on filter paper is reported. (gaa-science.com)
The validity of the method was demonstrated by analysing the neonatal blood spot of a patient affected by guanidinoacetate methyltransferase (GAMT) deficiency. (gaa-science.com)
In conclusion, IP-10 detection in dried plasma is a simple and safe method that would help improve LTBI management. (uab.cat)

On initial screening, which often involves measuring the concentration of galactose in blood, classic galactosemia may be indistinguishable from other inborn errors of galactose metabolism, including galactokinase deficiency and galactose epimerase deficiency. (wikipedia.org)
A blood sample was drawn on Day 2 to measure 5-FU concentration. (oncotarget.com)
Considering that blood alcohol concentration (BAC) may not be reliable, alternative metabolic products of alcohol has arisen after alcohol consumption. (nofor.org)

Porphyrins and heme components are extracted from whole blood into a 4:1 mixture of ethyl acetate-acetic acid. (cdc.gov)
Detection of cryptosporidium oocysts by acid fast stain. (tamu.edu)

A 1 μL aliquot of the sample was then introduced to the thermal desorption unit, dried, and thermally desorbed directly to the gas chromatograph. (rsc.org)
3. Add the appropriate unit of oxidase to the sample solution, and then add an equal volume of detection solution. (whdsbio.cn)
Once the sample is extracted, the next steps are in amplification and detection. (clpmag.com)
The sample was hydrolyzed at 100 C in dry-block for 25 min, then cooled to room temperature and extracted with dichloromethane-acetone (4:1,v/v) as described above. (medline.ru)

Nonetheless, establishing a clinical association between pathogen detection and illness remains challenging. (cdc.gov)
Full urinalysis (gross examination, specific gravity via refractometer, biochemical analysis with confirmatory testing, and microscopic evaluation of a wet mount) as well as air-dried slide examination by a clinical pathologist. (tamu.edu)

Its synthesis and secretion is, in turn, controlled by plasma osmolality, as well as by blood pressure and volume ( Sladek, 2000 ). (jneurosci.org)

Determination of Detection steroids cause a lot makes it so mild, then we can truly begin to appreciate. (sideload.com)

The agreement among in vitro tests was substantial and IP-10 levels in direct and dried plasma were strongly correlated (r = 0.897). (uab.cat)

Confirmatory testing would include measurement of enzyme activity in red blood cells, determination of Gal-1-P levels in the blood, and mutation testing. (wikipedia.org)

This review offers an insight on the methods used in the metabolomics analysis (LC-MS, GC-MS, HPLC-DAD, NMR) of several natural matrices with protective health potential, with special emphasis on the determination of phenolics profiles, once these represent the most abundant and widely spread class of plant natural compounds, additionally exhibiting interesting biological activities. (eurekaselect.com)
Previous studies on detection of Trypanosoma species in Somali camels have only been performed during the 1990s using standard trypanosome detection methods (STDM). (researchgate.net)
during the 1990s using standard trypanosome detection methods (STDM). (researchgate.net)
Hence, this review reports possible methods and strategies for the fast and reliable detection of ZIKV with particular emphasis on current updates, knowledge, and new hypotheses that might be helpful for medical professionals in poor and developing countries that urgently need to address this problem. (frontiersin.org)

Epi reduced blood pressure (BP), which persisted for two weeks post treatment. (mdpi.com)
Blood collected from pediatric malaria patients on treatment form amodiaquine plus artesunate (AQ=AS) has been analysed for drug levels. (mak.ac.ug)

IP-10 levels were determined in direct and dried plasma by an in-house ELISA. (uab.cat)
Regarding TB patients, 100.0%, 91.7% and 75.0% were positive for IFN-γ detection and IP-10 detection in direct and dried plasma, respectively. (uab.cat)
In contacts, 69.1%, 60.3% and 48.5% had positive results after IFN-γ and IP-10 in direct and dried plasma, respectively. (uab.cat)

Molecular testing focuses on PCR amplification, with end-point, PCR-based multiplex testing as well as real-time PCR, where detection and amplification takes place in a single tube, reducing detection to hours (in some cases, to minutes). (clpmag.com)

No interferences were recorded from endogenous blood components or a number of commonly occurring alcohols. (rsc.org)

Highly Sensitive and Selective Detection of the Antidepressant Amitriptyline Using a Functionalised Graphene-Based Sensor. (bournemouth.ac.uk)

The collection with drug testing in oral ﬂ uid,8 and detection times and time is typically one to three minutes, however this can vary as pharmacokinetics of selected drugs.9,10 discussed earlier. (nomedicalcare.com)
Capillary blood is collected by a prick in the finger, dropped onto the EUROIMMUN blood collection card and air-dried. (euroimmun.de)

This research provides critical new insights regarding SERS signal variation and offers a simple methodology for reliable determination of the SERS enhancement factors. (acs.org)

Anatomy10

Organisms7

Diseases3

Chemicals and Drugs56

Analytical, Diagnostic and Therapeutic Techniques and Equipment126

Psychiatry and Psychology2

Phenomena and Processes34

Disciplines and Occupations12

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture6

Information Science13

Named Groups1

Health Care22

Spots4

Urine13

Chromatographic3

Venous blood3

HPLC-UV1

Antibodies6

Concentrations2

Diagnosis4

Samples15

Metabolites2

Quantification2

Limits3

Fluorescence1

Quantitative1

Analytical3

Peripheral blood3

Viral4

Serum1

Qualitative1

Causative agent2

Pathogenic1

Rapid detection2

Antibody2

EDTA1

Assays1

Specimen1

Colorimetric1

Laboratory2

Biomarkers1

Hemorrhagic1

Reagents2

Forensic cases1

Infection2

Newborn2

Mass spectrometry2

Method4

Concentration3

Acid2

Sample4

Clinical2

20001

Mild1

Vitro1

Enzyme1

Methods4

Treatment2

Plasma3

Molecular1

Commonly1

Sensitive1

Collection2

Reliable1